New Triterpenes from Maytenus robusta: Structural Elucidation Based on NMR Experimental Data and Theoretical Calculations

Abstract

Leaves of Maytenus robusta (Celastraceae) were subjected to phytochemical investigation mainly directed at the isolation of pentacyclic triterpenes. The compounds friedelin (1), β-friedelinol (2), 3-oxo-21β-H-hop-22(29)-ene (7), 3,4-seco-friedelan-3,11β-olide (8), 3β-hydroxy-21β-H-hop-22(29)-ene (9), 3,4-seco-21β-H-hop-22(29)-en-3-oic acid (10), 3,4-seco-friedelan-3-oic acid (11), and sitosterol were identified in the hexane extract of M. robusta leaves. Compounds 8 and 9 are described herein for the first time. The structure and stereochemistry of both compounds were experimentally established by IR, HRLC-MS, and 1D (¹H, ¹³C, and DEPT 135) and 2D (HSQC, HMBC and COSY) NMR data and supported by correlations with carbon chemical shifts calculated using the DFT method (BLYP/6-31G* level). Compounds 7 and 10 are also described for the first time, and their chemical structures were established by comparison with NMR data of similar structures described in the literature and correlations with BLYP/6-31G* calculated carbon chemical shifts. Compound 9, a mixture of 11 and sitosterol, and 3β,11β-dihydroxyfriedelane (4) were evaluated by the Ellman’s method and all these compounds showed acethylcholinesterase inhibitory properties.

1. Introduction

Secondary metabolites are isolated from plants and animals, and many of them have been used as sources of derivatives with a large spectrum of biological activities [1], including effects in the treatment of Alzheimer’s disease (AD). AD is a progressive neurodegenerative disorder characterized by a decline in memory and cognitive abilities. About 34 million people around the World have AD, being the major cause of dementia in elderly people [2]. Acetylcholinesterase (AChE) inhibitors are a group of drugs frequently investigated for the symptomatic treatment of AD [3]. Alternatively, the literature also describes some relationships between pentacyclic triterpenes and treatments for AD [4,5,6,7].

Some biologically active alkaloids, phenolic compounds, and terpenes have been isolated from some species of Maytenus (Celastraceae) [8,9,10,11,12,13,14]. The triterpenes friedelin (3-oxofriedelane; 1), β-friedelinol (3β-hydroxyfriedelane; 2), and 3,15-dioxo-21α-hydroxyfriedelane (3) were isolated from the leaves of Maytenus robusta [15,16,17] (Figure 1). Moreover, we recently studied a white precipitate obtained from the hexane extract of the leaves of M. robusta, resulting in isolation and identification of a new triterpene 3β,11β-dihydroxyfriedelane (4) and the known triterpenes 1, 2, 3-oxo-29-hydroxyfriedelane (5), and 3-oxo-11β-hydroxyfriedelane (6) [18] (Figure 1).

Figure 1. Chemical structure of the triterpenes 1 to 6 previously isolated from the leaves of M. robusta.

Figure 1. Chemical structure of the triterpenes 1 to 6 previously isolated from the leaves of M. robusta.

Species of the genus Maytenus are used in the traditional Brazilian medicine for the treatment of gastric ulcers [19], inflammations, and diarrhea [20], as antimicrobial [21,22], antitumor [23,24], insecticidal agents [25], and for other purposes [14,20]. The antiulcerogenic and antinociceptive activities of M. robusta were previously investigated [16,17], but the AChE inhibitory activity of the triterpenes isolated from the species of Maytenus has not been tested to date.

Therefore, the present work describes a phytochemical study of the leaves of M. robusta that was directed to the isolation of triterpenes and analysis of their AChE inhibitory activity. The leaf hexane extract of M. robusta provided the triterpenes 1, 2, 3-oxo-21β-H-hop-22(29)-ene (7), 3,4-seco-friedelan-3,11β-olide (8), 3β-hydroxy-21β-H-hop-22(29)-ene (9), 3,4-seco-21β-H-hop-22(29)-en-3-oic acid (10), and 3,4-seco-friedelan-3-oic acid (11) and the steroid sitosterol (Figure 2).

Figure 2. Chemical structure of triterpenes isolated from the leaves of M. robusta (compounds 7 to 11), including similar chemical structures of triterpenes (compounds 12 to 15) with NMR data described in the literature.

Figure 2. Chemical structure of triterpenes isolated from the leaves of M. robusta (compounds 7 to 11), including similar chemical structures of triterpenes (compounds 12 to 15) with NMR data described in the literature.

Triterpenes 8 and 9 are described for the first time in the literature. Their structure and stereochemistry were deduced from experimental IR, HRLC-MS, and 1D (¹H, ¹³C, and DEPT 135) and 2D (HSQC, HMBC, and COSY) NMR analyses and theoretical methodology based on carbon chemical shifts calculated using the BLYP/6-31G* level of theory.

Compounds 7 and 10 are also new in the literature and were isolated as binary mixtures with 1 and 11, respectively (Figure 2). The chemical structure of 7 and 10 were established based on 1D NMR analyses, comparison with the NMR spectral data of the terpenes 3α-hydroxy-21β-H-hop-22(29)-ene (12) [26], 3-oxo-21α-H-hop-22(29)-ene (13) [27], 3,4-seco-lup-20(29)-en-3-oic acid (14) [28], and 21β-H-hop-22(29)-ene (15) [26] (Figure 2), and correlations with carbon chemical shifts calculated using BLYP/6-31G*. The chemical structure of compounds 1, 2, 11 and sitosterol were based on comparisons with the NMR data available in the literature. The triterpenes 4, 9, and 11 were submitted to the Ellman’s bioassay [29,30] and exhibited AChE inhibitory properties.

2. Results and Discussion

2.1. Structural Analysis

2.1.1. Compounds 1 and 7

The hexane extract (HE) fractions eluted with 9:1 hexane-chloroform provided a white solid. The IR spectrum of this solid shows two intense absorptions at 1,714 and 1,701 cm⁻¹ which are attributed to carbonyl groups. The ¹³C-NMR spectrum shows two groups of 30 signals, each with significant differences in intensity. The ¹³C-NMR data of the group of high-intensity signals (named triterpene 1) present a signal at δ_C 213.2 which is characteristic of a carbonyl carbon. The NMR data are similar to the corresponding ones described in the literature for the triterpene friedelin [31]. In turn, the ¹³C-NMR data of the group of low-intensity signals (named triterpene 7) present a signal at δ_C 218.2 which is also characteristic of a carbonyl carbon. Two other signals at δ_C 148.6 and 110.1 (non-hydrogenated and methylene carbon atoms, respectively) are characteristic of alkenyl carbon atoms. The ¹H-NMR data show a signal at δ_H 4.78 (integrated for two hydrogen atoms) which is also characteristic of an alkenyl group. These signals are in agreement with a hopane-type skeleton containing a carbonyl carbon. The ¹³C-NMR data of 7 were compared with the hopane-type skeleton data compiled in the literature for 12 and 13 (see Figure 2 and Table 1). Triterpene 7 only differs in relation to the substituent at C-3 of the ring A of 12 and the stereochemistry of the C-21 in the ring E of 13. In fact, the NMR data of C-1 to C-15 of 13 are very similar to the corresponding data of 7. On the other hand, the NMR data of C-14 to C-30 (except for C-23 and C-24) of 12 are very similar to the corresponding data of 7. As result, it can be proposed that the chemical structure of 7 is a combination of the rings A–C of 13 and rings C–E of 12. The chemical structure of 7 is thus in agreement with that of the compound 3-oxo-21β-H-hop-22(29)-ene, a triterpene which was not yet described in the literature. Moreover, the intensity and integration of the carbonyl carbon atom signals based on quantitative ¹³C-NMR analysis indicates a mixture 2:1 of compounds 1 and 7, respectively. BLYP/6-31G* geometry optimization calculations were carried out for 7 with a starting geometry based on the stereochemistry proposed to 3-oxo-21β-H-hop-22(29)-ene (see Figure 2). The most stable optimized geometry of 7 (E = −1246.52767272 a.u.) presents rings A–D in the chair conformation and ring E in the envelope one. Moreover, the C-29 of the allyl group is positioned close to the methyl group at C-28. Carbon chemical shift calculations were carried out on the optimized geometry of 7 at the same level of theory. Correlations between calculated and experimental ¹³C-NMR carbon chemical shift values of the data of 7 (Table 1) provided high correlation coefficient (R² = 0.99330) and slope of the R² curve (α = 0.91728). These theoretical results are also in agreement with the stereochemistry of 3-oxo-21β-H-hop-22(29)-ene proposed for 7.

Table 1. ¹³C-NMR data of triterpene 7, compared with the corresponding data described in the literature for 12 [26] and 13 [27], and ¹³C-NMR data of triterpene 10, compared with the corresponding data described in the literature for 14 [28] and 15 [26].

Carbon	Compound/ δC
	7	12	13	10	14	15
1	39.6	33.2	39.6	33.8	33.8	40.4
2	34.2	25.4	34.2	28.4	28.3	18.7
3	218.2	76.3	217.9	180.1	180.6	42.1
4	47.4	37.2	47.4	25.4	25.9	33.3
5	54.9	50.1	54.9	40.7	40.7	56.1
6	19.7	18.3	19.8	18.3	25.0	18.7
7	33.7	33.2	32.7	32.0	29.6	33.3
8	41.6	41.9	41.6	40.0	40.5	42.1
9	49.6	49.5	49.7	47.2	47.1	50.4
10	36.8	37.5	36.8	41.5	39.1	37.5
11	21.6	20.9	21.6	21.7	21.5	20.9
12	23.9	23.9	23.9	24.0	24.9	24.0
13	49.6	48.9	48.8	49.6	38.0	49.5
14	42.1	42.1	42.3	42.5	42.9	41.9
15	32.6	33.6	32.7	32.7	27.3	33.6
16	21.6	21.6	20.8	21.8	35.4	21.6
17	54.9	54.9	53.9	54.9	43.1	54.9
18	44.7	44.7	44.2	44.8	48.1	44.8
19	41.9	41.9	40.2	42.0	47.8	41.9
20	27.3	27.4	27.8	27.4	150.6	27.4
21	46.4	46.5	47.9	46.5	29.7	46.5
22	148.6	148.7	148.0	148.7	39.8	148.7
23	26.6	28.6	26.6	19.4	19.5	33.4
24	21.1	22.5	21.1	18.8	18.7	21.6
25	15.7	15.7	15.7	16.5	15.8	15.9
26	16.4	16.6	16.5	24.8	20.0	16.7
27	16.6	16.8	16.5	16.5	14.3	16.7
28	16.1	16.1	15.2	16.2	17.9	16.1
29	110.1	110.1	109.5	110.1	109.3	110.1
30	25.0	25.3	19.7	25.0	19.2	25.0

Table 1. ¹³C-NMR data of triterpene 7, compared with the corresponding data described in the literature for 12 [26] and 13 [27], and ¹³C-NMR data of triterpene 10, compared with the corresponding data described in the literature for 14 [28] and 15 [26].

Carbon	Compound/ δC
	7	12	13	10	14	15
1	39.6	33.2	39.6	33.8	33.8	40.4
2	34.2	25.4	34.2	28.4	28.3	18.7
3	218.2	76.3	217.9	180.1	180.6	42.1
4	47.4	37.2	47.4	25.4	25.9	33.3
5	54.9	50.1	54.9	40.7	40.7	56.1
6	19.7	18.3	19.8	18.3	25.0	18.7
7	33.7	33.2	32.7	32.0	29.6	33.3
8	41.6	41.9	41.6	40.0	40.5	42.1
9	49.6	49.5	49.7	47.2	47.1	50.4
10	36.8	37.5	36.8	41.5	39.1	37.5
11	21.6	20.9	21.6	21.7	21.5	20.9
12	23.9	23.9	23.9	24.0	24.9	24.0
13	49.6	48.9	48.8	49.6	38.0	49.5
14	42.1	42.1	42.3	42.5	42.9	41.9
15	32.6	33.6	32.7	32.7	27.3	33.6
16	21.6	21.6	20.8	21.8	35.4	21.6
17	54.9	54.9	53.9	54.9	43.1	54.9
18	44.7	44.7	44.2	44.8	48.1	44.8
19	41.9	41.9	40.2	42.0	47.8	41.9
20	27.3	27.4	27.8	27.4	150.6	27.4
21	46.4	46.5	47.9	46.5	29.7	46.5
22	148.6	148.7	148.0	148.7	39.8	148.7
23	26.6	28.6	26.6	19.4	19.5	33.4
24	21.1	22.5	21.1	18.8	18.7	21.6
25	15.7	15.7	15.7	16.5	15.8	15.9
26	16.4	16.6	16.5	24.8	20.0	16.7
27	16.6	16.8	16.5	16.5	14.3	16.7
28	16.1	16.1	15.2	16.2	17.9	16.1
29	110.1	110.1	109.5	110.1	109.3	110.1
30	25.0	25.3	19.7	25.0	19.2	25.0

2.1.2. Compound 2

The HE fractions eluted with 7:3 hexane-chloroform provided a white solid (named triterpene 2). The IR spectrum shows an absorption at 3471 cm⁻¹, which is attributed to a hydroxyl group. The absorptions at 1384 and 1172 cm⁻¹ can be attributed to the asymmetric and symmetric C–O stretches, respectively. The ¹H-NMR and ¹³C-NMR spectra shows a large signal at δ_H 3.74 and a signal at δ_C 72.8 which are characteristic of a carbinolic carbon. The NMR data are similar to the corresponding ones described in the literature for the triterpene β-friedelinol [32].

2.1.3. Compound 8

The HE fractions eluted with 3:2 hexane-chloroform provided a white solid with molecular formula C₃₀H₅₀O₂ as deduced from HR-APCIMS (m/z 443.3936 [M+H]⁺, calc. 443.3922), named triterpene 8. The IR spectrum of 8 shows absorptions at 1726, 1288, and 1024 cm⁻¹ (attributed to the C=O and asymmetric and symmetric C–O stretches, respectively) which are characteristic of a lactone group. The ¹H-NMR spectrum of 8 shows a double-doublet signal at δ_H 4.25 (J = 11.2 and 5.2 Hz, integrating for one hydrogen atom) which is characteristic of a hydrogen bonded to an oxygenated sp³ carbon and neighboring a methylene carbon in a cyclic system. The multiplet signal at δ_H 2.65–2.49 (integrating for two hydrogen atoms) can be attributed to the diastereotopic hydrogen atoms of a methylene group that is bonded to a carbonyl group and methylene group in a cyclic system. The seven singlet signals at δ_H 1.18, 1.09, 1.04, 0.99, 0.97, 0.95, and 0.79 can be attributed to methyl groups bonded to non-hydrogenated carbon atoms. The triplet signal at δ_H 0.78 (J = 7.4 Hz) can be attributed to a methyl group bonded to a methylene carbon. The ¹³C-NMR spectrum of 8 shows a non-hydrogenated carbon signal at δ_C 175.6 which is attributed to the carbonyl carbon of a lactone group. The signal at δ_C 84.1 can be attributed to a methynic sp³ carbon bonded to the oxygen of the lactone group. The ¹³C-NMR spectrum also shows six non-hydrogenated (δ_C 42.9, 40.7, 37.9, 36.8, 30.0, and 28.1), three methynic (δ_C 58.2, 52.6, and 42.6), 11 methylenic (δ_C 39.2, 38.8, 37.6, 36.1, 35.9, 35.3, 34.5, 32.7, 32.1, 19.1, and 18.0), and eight methylic (δ_C 34.9, 31.7, 32.0, 22.1, 19.9, 19.3, 13.6, and 7.7) carbon atoms. The COSY contour map of 8 shows correlations of the signal at δ_H 2.62 (H-2β) with the signal at δ_H 1.73 (H-1α); the signal at δ_H 4.25 (H-11) with the signals at δ_H 1.67 (H-12β) and 1.61 (H-12α); the signal at δ_H 1.48 (H-21α) with the signal at δ_H 0.97 (H-22β); and the signal at δ_H 0.78 (H-23) with the signals at δ_H 1.32 (H-4β) and 1.18 (H-4α). The HMBC contour map shows correlations of the hydrogen signals at δ_H 1.73 (H-1α) and 1.58 (H-1β) with the carbon signals at δ_C 175.6 (C-3), 58.2 (C-10), and 34.5 (C-2). The hydrogen signals at δ_H 2.62 (H-2β) and 2.52 (H-2α) correlate with the carbon signals at δ_C 175.6 (C-3), 58.2 (C-10), and 19.1 (C-1). The hydrogen signal at δ_H 4.25 (H-11) correlates with the carbon signals at δ_C 58.2 (C-10), 37.6 (C-12), and 13.6 (C-25). The hydrogen signals at δ_H 1.67 (H-12β) and 1.61 (H-12α) correlate with the carbon signal at δ_C 84.1 (C-11). The hydrogen signal at δ_H 0.78 (H-23) correlates with the carbon signals at δ_C 36.8 (C-5) and 36.1 (C-4). The hydrogen signal at δ_H 0.79 (H-24) correlates with the carbon signals at δ_C 58.2 (C-10), 38.8 (C-6), 36.8 (C-5), and 36.1 (C-4). The hydrogen signal at δ_H 0.97 (H-25) correlates with the carbon signals at δ_C 84.1 (C-11), 58.2 (C-10), 52.6 (C-8), and 42.9 (C-9). The NMR data of 8 are in agreement with the data of the triterpene 3,4-seco-friedelan-3,11β-olide. In fact, the ¹³C-NMR data of 8 were compared with the seco-friedelane-type skeleton data compiled in the literature for 11 [33], which only presented significant differences in the functionalities at C-3 and C-11. The NMR data of C-14 to C-23 and C-26 to C-30 of 8 are very similar to the corresponding data of 11 (Table 2). BLYP/6-31G* geometry optimization calculations were carried out for 8 with a starting geometry based on the stereochemistry proposed for 3,4-seco-friedelan-3,11-olide (see Figure 2). Two stereochemistry possibilities were considered for carbon C-11: H-11α or H-11β.

Table 2. NMR data of 8 and corresponding data described in the literature for 11 [33].

Triterpene 8						Compound 11
Atom	Type	δC	δH	HMBC	COSY	δC
1	CH2	19.1	1.73 (H α); 1.58 (Hβ)	H-2 α; H-2β		33.2
2	CH2	34.5	2.52 (H α); J = 13.2; t 2.62 (H β); J = 13.8 and 6.6 Hz; dd	H-1 α; H-1β	H-1 α	25.4
3	C	175.6		H-1α; H-1 β; H-2β		76.3
4	CH2	36.1	1.18 (Ha); 1.32 (Hb)	H-23; H-24		37.2
5	C	36.8		H-23; H-24		50.1
6	CH2	38.8	1.38 (H α ); 1.59 (H β)	H-24		18.3
7	CH2	18.0	1.51 (H α and Hβ)			33.2
8	CH	52.6	1.34 (H α)	H-25; H-26		41.9
9	C	42.9		H-25		49.5
10	CH	58.2	1.25 (H α)	H-1 α; H-1β; H-2α; H-2β; H-11; H-24; H-25		37.5
11	CH	84.1	4.25 (H α); J = 5.2 and 11.2 Hz; dd	H-12 α; H-12β; H-25	H-12	20.9
12	CH2	37.6	1.61 (H α ); 1.67 (H β)	H-11; H-27		23.9
13	C	40.7		H-26; H-27		48.9
14	C	37.9		H-26		42.1
15	CH2	32.1	1.54 (H α and Hβ)	H-26		33.6
16	CH2	35.9	1.39 (H β ); 1.56 (H α)	H-28		21.6
17	C	30.0				54.9
18	CH	42.6	1.61 (H β)	H-27; H-28		44.7
19	CH2	35.3	1.39 (H α ); 1.24 (H β)	H-29; H-30		41.9
20	C	28.1		H-29; H-30		27.4
21	CH2	32.7	1.48 (H α);	H-29; H-30	H-22 β	46.5
22	CH2	39.2	1.49 (H α ); 0.97 (H β)	H-28		148.7
23	CH3	7.7	0.78; J = 7.4 Hz; t		H-4	28.6
24	CH3	22.1	0.79; s			22.5
25	CH3	13.6	0.97; s	H-11		15.7
26	CH3	19.9	1.04; s			16.6
27	CH3	19.3	1.09; s			16.8
28	CH3	32.0	1.18; s			16.1
29	CH3	34.9	0.95; s	H-30		110.1
30	CH3	31.7	0.99; s	H-29		25.3

Table 2. NMR data of 8 and corresponding data described in the literature for 11 [33].

Triterpene 8						Compound 11
Atom	Type	δC	δH	HMBC	COSY	δC
1	CH2	19.1	1.73 (H α); 1.58 (Hβ)	H-2 α; H-2β		33.2
2	CH2	34.5	2.52 (H α); J = 13.2; t 2.62 (H β); J = 13.8 and 6.6 Hz; dd	H-1 α; H-1β	H-1 α	25.4
3	C	175.6		H-1α; H-1 β; H-2β		76.3
4	CH2	36.1	1.18 (Ha); 1.32 (Hb)	H-23; H-24		37.2
5	C	36.8		H-23; H-24		50.1
6	CH2	38.8	1.38 (H α ); 1.59 (H β)	H-24		18.3
7	CH2	18.0	1.51 (H α and Hβ)			33.2
8	CH	52.6	1.34 (H α)	H-25; H-26		41.9
9	C	42.9		H-25		49.5
10	CH	58.2	1.25 (H α)	H-1 α; H-1β; H-2α; H-2β; H-11; H-24; H-25		37.5
11	CH	84.1	4.25 (H α); J = 5.2 and 11.2 Hz; dd	H-12 α; H-12β; H-25	H-12	20.9
12	CH2	37.6	1.61 (H α ); 1.67 (H β)	H-11; H-27		23.9
13	C	40.7		H-26; H-27		48.9
14	C	37.9		H-26		42.1
15	CH2	32.1	1.54 (H α and Hβ)	H-26		33.6
16	CH2	35.9	1.39 (H β ); 1.56 (H α)	H-28		21.6
17	C	30.0				54.9
18	CH	42.6	1.61 (H β)	H-27; H-28		44.7
19	CH2	35.3	1.39 (H α ); 1.24 (H β)	H-29; H-30		41.9
20	C	28.1		H-29; H-30		27.4
21	CH2	32.7	1.48 (H α);	H-29; H-30	H-22 β	46.5
22	CH2	39.2	1.49 (H α ); 0.97 (H β)	H-28		148.7
23	CH3	7.7	0.78; J = 7.4 Hz; t		H-4	28.6
24	CH3	22.1	0.79; s			22.5
25	CH3	13.6	0.97; s	H-11		15.7
26	CH3	19.9	1.04; s			16.6
27	CH3	19.3	1.09; s			16.8
28	CH3	32.0	1.18; s			16.1
29	CH3	34.9	0.95; s	H-30		110.1
30	CH3	31.7	0.99; s	H-29		25.3

The optimized geometry of 3,4-seco-friedelan-3,11β-olide shows lower energy than the optimized geometry of 3,4-seco-friedelan-3,11α-olide (E^{electr.−nucl.} = −1322.96006656 and −1322.94516689 a.u., respectively), corresponding to ΔE^{electr.−nucl.} = 9.34 kcal/mol. The geometry of 3,4-seco-friedelan-3,11β-olide, which does not have the ring A, presents rings B and C in the chair conformation and rings D and E in the boat conformation. Carbon chemical shift calculations were carried out for both the optimized geometries at the same level of theory (BLYP/6-31G*). Correlations between values of calculated carbon chemical shifts and experimental ¹³C-NMR data of 8 (Table 2) provided a higher correlation coefficient and slope (R² = 0.98055 and α = 0.90931) for 3,4-seco-friedelan-3,11β-olide than the corresponding values for 3,4-seco-friedelan-3,11α-olide (R² = 0.97441 and α = 0.89006). These theoretical results are in agreement with the stereochemistry of 3,4-seco-friedelan-3,11β-olide proposed for 8, a triterpene not yet described in the literature.

2.1.4. Compound 9

The HE fractions eluted with 3:2 hexane-chloroform also provided a white solid with molecular formula C₃₀H₅₀O as deduced from HR-APCIMS (m/z 409.3855 [M+H−18]⁺, calc. 409.3834), named triterpene 9. The IR spectrum shows an absorption at 3488 cm⁻¹ which is characteristic of a hydroxyl group. The weak absorption at 1640 cm⁻¹ can be attributed to an alkenyl group. The absorptions at 1372 and 1050 cm⁻¹ can be attributed to the asymmetric and symmetric C–O stretches, respectively. The ¹H-NMR spectrum shows a signal at δ_H 4.79 (integrating for two hydrogen atoms) which is characteristic of the alkenyl hydrogen atoms of a methylenic carbon. Then, the other alkenyl carbon is non-hydrogenated. The multiplet at δ_H 3.25–3.21 can be attributed to a carbinolic hydrogen. The multiplet at δ_H 2.71–2.64 corresponds to a hydrogen neighboring an alkenyl group. The singlets at δ_H 1.75, 1.02, 0.97, 0.94, 0.83, 0.81, and 0.73 can be attributed to methylic hydrogen atoms. The ¹³C-NMR spectrum shows signals at δ_C 148.6 (non-hydrogenated carbon) and 110.2 (methylenic carbon) which are characteristic of an alkenyl group. The signal at δ_C 78.4 is characteristic of a carbinolic carbon. The ¹³C-NMR spectrum also shows other signals which are attributed to five non-hydrogenated (δ_C 44.8, 42.1, 41.7, 39.0, and 37.2), five methynic (δ_C 55.3, 54.9, 50.4, 49.5, and 46.5), 10 methylenic (δ_C 41.9, 38.9, 33.7, 33.4, 27.5, 27.4, 24.0, 21.7, 21.1, and 18.5), and seven methylic (δ_C 28.2, 25.0, 16.7, 16.6, 16.1, 15.9, and 15.7) carbon atoms (see Table 3). The COSY contour map of 9 shows correlations of the signal at δ_H 3.23 (H-3) with the signal at δ_H 1.65 (H-2); the signals at δ_H 1.53 (H-6β) and 1.40 (H-6α) with the signal at δ_H 0.71 (H-5); the signals at δ_H 1.97 (H-20β) and 1.86 (H-20α) with the signals at δ_H 1.61 (H-19α) and 1.04 (H-19β); the signal at δ_H 2.67 (H-21) with the signals at δ_H 1.39 (H-17), 1.97 (H-20β), and 1.86 (H-20α); the signal at δ_H 4.79 (H-29) with the signals at δ_H 2.67 (H-21) and 1.75 (H-30). The HMBC contour map shows correlations of the hydrogen signal at δ_H 3.23 (H-3) with the carbon signals at δ_C 39.0 (C-4), 28.2 (C-23), and 15.7 (C-24); the hydrogen signal at δ_H 2.67 (H-21) with the carbon signals at 148.6 (C-22), 110.2 (C-29), δ_C 54.9 (C-17), 44.8 (C-18), 27.4 (C-20), and 25.0 (C-30); the hydrogen signal at δ_H 4.79 (H-29) with the carbon signals at 148.6 (C-22), δ_C 46.5 (C-21), and 25.0 (C-30); the hydrogen signal at δ_H 1.75 (H-30) with the carbon signals at 148.6 (C-22), 110.2 (C-29), and δ_C 46.5 (C-21).

Table 3. NMR data of triterpene 9 and corresponding data described in the literature for 12 [26].

Triterpene 9						Compound 12
Atom	Type	δC	δH	HMBC	COSY	δC
1	CH2	38.9	0.94 (H α);1.70 (Hβ)	H-25		33.2
2	CH2	27.5	1.63 (H α and Hβ)			25.4
3	CH	78.4	3.23 (H α); m	H-23; H-24	H-2	76.3
4	C	39.0		H-3; H-23; H-24		37.2
5	CH	55.3	0.69 (H α)	H-23; H-24; H-25		50.1
6	CH2	18.5	1.40 (H α); 1.53 (Hβ)		H-5	18.3
7	CH2	33.4	1.47 (H α); 1.62 (Hβ)	H-26		33.2
8	C	41.7		H-26; H-27		41.9
9	CH	50.4	1.24 (H α)	H-25; H-26		49.5
10	C	37.2		H-5; H-25		37.5
11	CH2	21.1	1.51 (H α); 1.32 (Hβ)			20.9
12	CH2	24.0	1.43 (H α); 1.49 (Hβ)			23.9
13	CH	49.5	1.37 (H β)	H-27; H-28		48.9
14	C	42.1		H-26; H-27		42.1
15	CH2	33.7	1.42 (H α); 1.24 (Hβ)	H-27		33.6
16	CH2	21.7	1.74 (H α); 1.65 (Hβ)			21.6
17	CH	54.9	1.39 (H β)	H-21; H-28		54.9
18	C	44.8		H-21; H-28		44.7
19	CH2	41.9	1.60 (H α); 1.04 (Hβ)	H-28		41.9
20	CH2	27.4	1.84 (H α); 1.97 (Hβ)	H-21	H-19	27.4
21	CH	46.5	2.67 (H β); J = 16.6 and 9.0 Hz; dd	H-29; H-30	H-17; H-20	46.5
22	C	148.6		H-21; H-29; H-30		148.7
23	CH3	28.2	1.02; s	H-3; H-24		28.6
24	CH3	15.7	0.81; s	H-3; H-23		22.5
25	CH3	16.7	0.83; s			15.7
26	CH3	15.9	0.94; s			16.6
27	CH3	16.7	0.97; s			16.8
28	CH3	16.1	0.73; s			16.1
29	CH2	110.2	4.79; s	H-21; H-30	H-30; H-21	110.1
30	CH3	25.0	1.75; s	H-21; H-29		25.3

Table 3. NMR data of triterpene 9 and corresponding data described in the literature for 12 [26].

Triterpene 9						Compound 12
Atom	Type	δC	δH	HMBC	COSY	δC
1	CH2	38.9	0.94 (H α);1.70 (Hβ)	H-25		33.2
2	CH2	27.5	1.63 (H α and Hβ)			25.4
3	CH	78.4	3.23 (H α); m	H-23; H-24	H-2	76.3
4	C	39.0		H-3; H-23; H-24		37.2
5	CH	55.3	0.69 (H α)	H-23; H-24; H-25		50.1
6	CH2	18.5	1.40 (H α); 1.53 (Hβ)		H-5	18.3
7	CH2	33.4	1.47 (H α); 1.62 (Hβ)	H-26		33.2
8	C	41.7		H-26; H-27		41.9
9	CH	50.4	1.24 (H α)	H-25; H-26		49.5
10	C	37.2		H-5; H-25		37.5
11	CH2	21.1	1.51 (H α); 1.32 (Hβ)			20.9
12	CH2	24.0	1.43 (H α); 1.49 (Hβ)			23.9
13	CH	49.5	1.37 (H β)	H-27; H-28		48.9
14	C	42.1		H-26; H-27		42.1
15	CH2	33.7	1.42 (H α); 1.24 (Hβ)	H-27		33.6
16	CH2	21.7	1.74 (H α); 1.65 (Hβ)			21.6
17	CH	54.9	1.39 (H β)	H-21; H-28		54.9
18	C	44.8		H-21; H-28		44.7
19	CH2	41.9	1.60 (H α); 1.04 (Hβ)	H-28		41.9
20	CH2	27.4	1.84 (H α); 1.97 (Hβ)	H-21	H-19	27.4
21	CH	46.5	2.67 (H β); J = 16.6 and 9.0 Hz; dd	H-29; H-30	H-17; H-20	46.5
22	C	148.6		H-21; H-29; H-30		148.7
23	CH3	28.2	1.02; s	H-3; H-24		28.6
24	CH3	15.7	0.81; s	H-3; H-23		22.5
25	CH3	16.7	0.83; s			15.7
26	CH3	15.9	0.94; s			16.6
27	CH3	16.7	0.97; s			16.8
28	CH3	16.1	0.73; s			16.1
29	CH2	110.2	4.79; s	H-21; H-30	H-30; H-21	110.1
30	CH3	25.0	1.75; s	H-21; H-29		25.3

The NMR analyses of 9 are in agreement with the data of the triterpene 3β-hydroxy-21β-H-hop-22(29)-ene. In fact, the ¹³C-NMR data of 9 were compared with the hopane-type skeleton data compiled in the literature for 12 [26], and seen to only present a significant difference in the stereochemistry at C-3. The NMR data of C-6 to C-8, C-10 to C12, C-14 to C-23, and C-27 to C-30 of 9 are very similar to the corresponding data of 12 (Table 3). BLYP/6-31G* geometry optimization calculations were carried out for 9 with a starting geometry based on the stereochemistry proposed to 3β-hydroxy-21β-H-hop-22(29)-ene (Figure 2). The most stable optimized geometry (E = −1247.70633974 a.u.) presents the rings A, B, C, and D in the chair conformation and the ring E in the envelope one. Moreover, the C-29 of the allyl group is positioned close to the methyl group at C-28. Carbon chemical shift calculations were carried out to the optimized geometry of 9 at the same level of theory (BLYP/6-31G*). Correlations between values of calculated carbon chemical shifts and experimental ¹³C-NMR data of 9 (Table 3) provided a high correlation coefficient (R² = 0.98817) and slope of the R² curve (α = 0.93702). These theoretical results are in agreement with the stereochemistry of 3β-hydroxy-21β-H-hop-22(29)-ene for 9, a triterpene not yet described in the literature.

2.1.5. Compounds 10 and 11

The HE fractions eluted with 1:1 hexane-chloroform provided a white solid. The IR spectrum of the solid shows a large absorption at 3250–2700 cm⁻¹ and an intense absorption at 1701 cm⁻¹ which are characteristic of a carboxylic acid group. Moreover, the absorptions at 1284 and 1049 cm⁻¹can be attributed to the asymmetric and symmetric C–O stretches, respectively. The ¹H-NMR spectrum shows a broad signal at δ_H 4.78 (integrating for two hydrogen atoms) which is characteristic of an alkenyl group. The ¹³C-NMR spectrum shows two groups of 30 signals, each with significant differences in intensity. The ¹³C-NMR data of the group of low-intensity signals (named triterpene 10) present a signal at δ_C 180.1 which is characteristic of a carboxylic carbon. The signals at δ_C 148.7 and 110.1 (non-hydrogenated and methylenic carbon atoms, respectively) are characteristic of an alkenyl group. The ¹³C-NMR data of 10 were compared with the corresponding data compiled in the literature for 14 and 15 (see Table 1). Triterpene 10 only differs in the position of the allyl group and the opening of the ring A in relation to 14 and 15, respectively (see Figure 2). The NMR data of C-1 to C-5, C-8, C-9, and C-11 of 14 are very similar to the corresponding data of 10. On the other hand, the NMR data of the C-12 to C-22 of 10 are very similar to the corresponding data of 15. As result, it can be proposed that the chemical structure of 10 is in agreement with the structure of the triterpene 3,4-seco-21β-H-hop-22(29)-en-3-oic acid, a triterpene which was not yet described in the literature. BLYP/6-31G* geometry optimization calculations were carried out for 10 with starting geometry based on the stereochemistry proposed for 3,4-seco-21β-H-hop-22(29)-en-3-oic acid (see Figure 2). The most stable optimized geometry (E = −1322.93835876 a.u.), which does not have the ring A, presents the rings B, C, and D in the chair conformation and ring E in the envelope one. Carbon chemical shift calculations were carried out to the optimized geometry of 10 at the same level of theory (BLYP/6-31G*). Correlations between values of calculated carbon chemical shifts and experimental ¹³C-NMR data of 10 (Table 1) provided high correlation coefficient (R² = 0.97833) and slope of the R² curve (α = 0.87424). These theoretical results are in agreement with the stereochemistry of 3,4-seco-21β-H-hop-22(29)-en-3-oic acid proposed for 10, a triterpene not yet described in the literature. In turn, the ¹³C-NMR data of the group of high intensity signals (named triterpene 11) also presents a signal characteristic of carboxyl carbon (at δ_C 178.2). The ¹³C-NMR data of 11 are similar to the corresponding data described in the literature for 3,4-seco-friedelan-3-oic acid [33]. Moreover, the intensity and integration of the carbonyl carbon atom signals based on quantitative ¹³C-NMR analysis indicates a mixture 2:3 of compounds 10 and 11, respectively.

2.2. In Vitro AChE Inhibitory Activity

The AChE activity was measured for the triterpenes 4, 9, and mixture of 11 and sitosterol which were previously obtained from the leaves of M. robusta. The calorimetric method of Ellman was adapted for 96-well microplates in the assays at 25 °C [30]. The triterpenes 4 and 9 showed (64 ± 3)% and (76 ± 1)% of inhibition, respectively. The mixture of triterpene 11 and sitosterol exhibited very significant results, i.e., (94 ± 1)% of inhibition.

3. Experimental

3.1. General Procedures

Uncorrected melting points were determined using a Microquímica apparatus, model MQAPF-302. Optical rotations were measured on a Perkin-Elmer Model 341 polarimeter using a 100 mm, 1.0 mL capacity cell. The IR spectra were taken on a Perkin Elmer-Spectrum One (ATR) spectrometer. The ¹H and ¹³C-NMR spectra at 400.129 and 100.613 MHz, respectively, as well as the COSY, HSQC, and HMBC experiments were performed on a Brüker DRX400 AVANCE spectrometer, using CDCl₃ or a mixture of CDCl₃/pyridine-d₅ as solvent, with direct or inverse probes and a field gradient. The chemical shifts were registered in ppm (δ) relative to TMS as the internal standard. The coupling constants (J) were registered in Hertz. HR-APCIMS spectra were acquired on a Shimadzu LCMS-IT-TOF system. Analyses were carried out using manual injection. The samples were dissolved in CHCl₃ and then diluted with MeOH. Column chromatography (CC) processes were carried out using silica gel 60 (70–230 Mesh). Thin layer chromatography (TLC) processes were carried out using precoated silica gel plates.

3.2. Phytochemical Methodology

3.2.1. Plant Material

Leaves of M. robusta were collected in June 2010 at the Parque Estadual do Itacolomi, in the City of Ouro Preto, State of Minas Gerais, Brazil. After botanical identification, the voucher specimen of M. robusta was deposited in the Herbário Professor José Badini, Universidade Federal de Ouro Preto, under the code OUPR: 25,559.

3.2.2. Extraction and Isolation of Constituents

Leaves of M. robusta were dried at room temperature until a constant weight was achieved (about one week) and finally powdered. A sample of this material (864.4 g) was submitted to extraction with hexane (3 L, 5 days, room temperature). A solid material (SM; 4.51 g) precipitated during solvent evaporation, being separated by filtration under reduced pressure. The SM was submitted to column chromatography using silica gel as the stationary phase (CCS) eluted with hexane, chloroform, ethyl acetate, and methanol in increasing polarity order. The triterpenes 1–6 (Figure 1) were obtained, as previously reported [18].

The rest of the hexane extract provided a viscous crude oil (HE; 32.0 g) after complete solvent evaporation. A part of HE (31.43 g) was submitted to CCS eluted with hexane, chloroform, ethyl acetate, and methanol in increasing polarity order. The HE fractions eluted with hexane-chloroform (9:1) were again submitted to CCS eluted with hexane and chloroform in increasing polarity order. The fractions eluted with hexane-chloroform (1:1) provided a white solid (13.5 mg) which was identified as a mixture of the triterpenes 1 and 7. The HE fractions eluted with hexane-chloroform (4:1) provided a white solid (624.0 mg) which was identified as triterpene 1. The HE fractions eluted with hexane-chloroform (7:3) provided a solid (566.1 mg) which was identified as triterpene 2.

The HE fractions eluted with hexane-chloroform (3:2) were again submitted to CCS eluted with hexane, chloroform, ethyl acetate, and methanol in increasing polarity order. The fractions hexane-chloroform (3:7) provided a white solid (14.1 mg) which was identified as triterpene 8. The fractions eluted with chloroform (289.0 mg) were submitted to CCS eluted with chloroform, providing a white solid (103.0 mg) which was identified as triterpene 9.

The HE fractions eluted with hexane-chloroform (1:1) were again submitted to CCS eluted with hexane, chloroform, ethyl acetate, and methanol in increasing polarity order. The fractions eluted with hexane-chloroform (3:7) provided a white solid (59.5 mg) which was identified as a mixture of the triterpenes 10 and 11. The fractions eluted with hexane-chloroform (1:9) provided a white solid (83.8 mg) which was identified as a mixture of 11 and the steroid sitosterol.

Friedelin (1): white solid (624.0 mg); m.p. 251–254 °C; IR (ATR; cm⁻¹) ν 2972, 2926, 2868, 1711, 1461, 1389, 1299, 1189, 1073, 1002, 982, and 924; ¹H-NMR (400 MHz; CDCl₃; ppm) δ_H 2.42–2.40 (multiplet; 1H), 2.38–2.37 (multiplet; 2H), 1.97–1.94 (multiplet; 1H), 1.77–1.34 (superposed signals; 21H), 1.29 (s; 3H), 1.18 (s; 3H), 1.05 (s; 3H), 1.01 (s; 3H), 0.95 (s; 3H), 0.89 (d, J = 6.4 Hz; 3H), 0.87 (s; 3H), and 0.73 (s; 3H); ¹³C-NMR (100 MHz; CDCl₃; ppm) δ_C 213.2 (C-3), 59.5 (C-10), 58.2 (C-4), 53.1 (C-8), 42.8 (C-18), 42.2 (C-5), 41.5 (C-2), 41.3 (C-6), 39.7 (C-14), 39.3 (C-22), 38.3 (C-13), 37.5 (C-9), 36.0 (C-16), 35.6 (C-11), 35.4 (C-19), 35.0 (C-29), 32.8 (C-21), 32.4 (C-15), 32.1 (C-28), 31.8 (C-30), 30.5 (C-12), 30.0 (C-17), 28.2 (C-20), 22.3 (C-1), 20.3 (C-26), 18.7 (C-27), 18.3 (C-7), 18.0 (C-25), 14.7 (C-24), and 6.8 (C-23). HR-APCIMS (m/z 427.3969 [M+H]⁺, calc. 427.3934).

β-Friedelinol (2): white solid (566.1 mg); m.p. 271–276 °C; IR (ATR; cm⁻¹) ν 3619, 3471, 2915, 2869, 1448, 1384, 1360, 1172, 1089, 1020, 1000, 979, and 920; ¹H-NMR (200 MHz; CDCl₃; ppm) δ_H3.74 (ls; H-3; 1H) and 2.50–0.80 (superposed signals); ¹³C-NMR (50 MHz; CDCl₃; ppm) δ_C 72.8 (C-3), 61.3 (C-10), 53.2 (C-8), 49.1 (C-4), 42.8 (C-18), 41.7 (C-6), 39.7 (C-14), 39.3 (C-22), 38.4 (C-13), 37.8 (C-5), 37.1 (C-9), 36.1 (C-2), 35.5 (C-16), 35.3 (C-11), 35.2 (C-19), 35.0 (C-29), 32.8 (C-21), 32.3 (C-15), 32.1 (C-28), 31.8 (C-30), 30.6 (C-12), 30.0 (C-17), 28.2 (C-20), 20.1 (C-26), 18.6 (C-27), 18.2 (C-25), 17.5 (C-7), 16.4 (C-24), 15.8 (C-1), and 11.6 (C-23); HR-APCIMS (m/z 411.3966 [M+H−18]⁺, calc. 411.3985).

3-Oxo-21β-H-hop-22(29)-ene (7): white solid (13.5 mg) obtained in mixture with 1; ¹H-NMR (200 MHz; CDCl₃; ppm) δ_H 4.78 (ls; 2H) and 2.43–0.73 (superposed signals). The ¹³C-NMR data of 7 are shown in Table 1.

3,4-seco-Friedelan-3,11β-olide (8): white solid (14.1 mg); m.p. 184–187 °C; IR (ATR; cm⁻¹) 2962, 2850, 1726, 1458, 1386, 1288, and 1024; ¹H (400 MHz; CDCl₃; ppm) δ_H 4.25 (dd, J = 11.2 and 5.2 Hz; H-11α), 2.62 (dd, J = 13.8 and 6.6 Hz; H-2β), 2.52 (t, J = 13.2 Hz; H-2α), 1.73 (H-1α), 1.67 (H-12β), 1.61 (H-12α and H-18β), 1.59 (H-6β), 1.58 (H-1β), 1.56 (H-16α), 1.54 (H-15α and H-15β), 1.51 (H-7α and H-7β), 1.49 (H-22α), 1.48 (H-21α), 1.39 (H-16β, H-17β, and H-19α), 1.38 (H-6α), 1.34 (H-8α), 1.32 (H-4b), 1.30 (H-21β), 1.25 (H-10α), 1.24 (H-19β), 1.18 (H-4a and H-28), 1.09 (H-27), 1.04 (H-26), 0.99 (H-30), 0.97 (H-22β and H-25), 0.95 (H-29), 0.79 (H-24), and 0.78 (t, J = 7.4 Hz; H-23); the ¹³C-NMR data of 8 are shown in Table 2; HR-APCIMS (m/z 443.3936 [M+H]⁺, calc. 443.3922).

3β-Hydroxy-21β-H-hop-22(29)-ene (9): white solid (103.0 mg); m.p. 217–221 °C; [α]²°_D= +46 (c = 2.22 × 10⁻³ M; CHCl₃); IR (ATR; cm⁻¹) ν 3488, 2931, 2870, 1640, 1445, 1372, 896, and 886; ¹H-NMR (400 MHz; CDCl₃/pyridine-d₅; ppm) δ_H 4.79 (s; H-29), 3.23 (m; H-3α), 2.67 (dd, J = 16.6 and 9.0 Hz; H-21), 1.97 (H-20β), 1.84 (H-20α), 1.75 (H-30), 1.74 (H-16α), 1.70 (H-1β), 1.65 (H-16β), 1.63 (H-2α and H-2β), 1.62 (H-7β), 1.60 (H-19α), 1.53 (H-6β), 1.51 (H-11α), 1.49 (H-12β), 1.47 (H-7α), 1.43 (H-12α), 1.42 (H-15α), 1.40 (H-6α), 1.39 (H-17β), 1.37 (H-13β), 1.32 (H-11β), 1.24 (H-9α and H-15β), 1.04 (H-19β), 1.02 (H-23), 0.97 (H-27), 0.94 (H-1α and H-26), 0.83 (H-25), 0.81 (H-24), 0.73 (H-28), and 0.69 (H-5α); the ¹³C-NMR data of 9 are shown in Table 3; HR-APCIMS (m/z 409.3855 [M+H−18]⁺, calc. 409.3834).

3,4-seco-21β-H-Hop-22(29)-en-3-oic acid (10): white solid obtained as a mixture with 11 (59.5 mg); ¹H-NMR (400 MHz; CDCl₃; ppm) δ_H 4.78 (ls; 2H), 2.67 (dd, J = 16.4 and 9.6 Hz), 2.38 (t, J = 8.7 Hz), 1.75–0.73 (superposed signals). The ¹³C-NMR data of 10 are shown in Table 1.

3,4-seco-Friedelan-3-oic acid (11) [33]: white solid obtained in mixture with 10 (59.5 mg); ¹H-NMR (400 MHz; CDCl₃; ppm) δ_H 2.38 (t, J = 8.7 Hz) and 1.75–0.73 (superposed signals). The ¹³C-NMR data of 11 are shown in Table 2.

3.3. Theoretical Methodology

Theoretical studies were carried out using the Gaussian 03 software package [34]. The geometries obtained from PM3 semi-empirical calculations were used as initial models in geometry optimizations employing DFT calculations with the Pople’s split valence basis set 6-31G*. BLYP exchange-correlation functional was used in DFT calculations. The optimized geometries were characterized as true minima on the potential energy surface (PES) when all harmonic frequencies were real. The electronic-nuclear energy (E) of the optimized geometries was given in atomic unit (Hartree). This theoretical methodology has been efficiently employed in the study of different organic compounds, including terpenes [35,36,37,38].

The optimized geometries were used to calculate carbon chemical shifts at the same levels of theory. Values of calculated carbon chemical shift (σ_C) were determined in relation to the corresponding calculated value for tetramethylsilane (σ_C 187.97). Correlations between σ_C values and experimental carbon chemical shifts (δ_C) were obtained using software package Origin™ Standard 7.5. The σ_C and δ_C values were plotted on the x and y axes, respectively. The σ_C/δ_C correlation curves were given as linear fits with correlation coefficients (R²) and slope of the R² curve (α) furnished by the program. The BLYP/6-31G* calculations usually give satisfactory results of carbon chemical shifts, as have been obtained in previous works [39,40,41].

3.4. In Vitro AChE Inhibitory Activity

The buffers A (50 mM Tris–HCl, pH 8, containing 0.1 M NaCl and 0.02 M MgCl₂.6H₂O), B (50 mM Tris–HCl, pH 8, containing 0.1% bovine serum albumin), and C (50 mM Tris–HCl, pH 8) were prepared to study the in vitro AChE inhibitory activity. This activity was measured using a 96-well microplate reader based on an adapted Ellman’s method [29,30]. The enzyme hydrolyzes the substrate acethylthiocholine. The obtained product, thiocholine, decomposes the Ellman’s reagent, 5,5-dithiobis-(2-nitrobenzoic acid) (DTNB), providing 2-nitrobenzoate-5-mercaptothiocholine and 5-thio-2-nitrobenzoate, which can be detected at 405 nm.

Volumes of acetylthiocholine iodide (25 μL, 15 mM in water), DTNB (125 μL, 3 mM in buffer A), buffer B (50 μL), and sample (25 μL, 10 mg/mL in MeOH diluted 10-fold with buffer C, resulting in a concentration of 1 mg/mL) were added into each well of a 96-well microplate. Instead of adding the sample solution, a volume of 25 μL of buffer C was employed to prepare the blank sample. The positive control was prepared under the same conditions, using physostigmine (eserine) as standard. Tests were carried out in quintuplicate. The absorbance was measured at 405 nm every 60 s by eight times using a Elisa Thermoplate microplate reader. After addition of 25 μL of acetylcholinesterase solution (0.226 U/mL in buffer B), the absorbance was again read every 60 s for ten times. The increase in absorbance relative to substrate spontaneous hydrolysis was corrected by reaction rate variation before and after addition of the enzyme. The inhibition percentage was calculated by comparing the rates of the sample with the blank.

4. Conclusions

The hexane extract of the leaves of M. robusta provided seven triterpenes. The triterpenes 1, 2, and 11 were also isolated in a previous phytochemical investigation. The triterpenes 8 and 9 are described for the first time in the literature. The triterpenes 7 and 10 are also new compounds, but both compounds were obtained as a mixture. Hopane and seco-hopane triterpenoids are not usual in species of the family Celastraceae. The combination of experimental NMR analyses with carbon chemical shift calculations was a useful procedure for the structural determination of these hopane and friedelane triterpenes. Compounds 4, 9, and the mixture of 11 and sitosterol showed acetylcholinesterase inhibitory properties. These compounds present hopane- and friedelane-type skeletons, suggesting biological potential of their derivatives for Alzheimer’s desease.