Functions of COP1/SPA E3 Ubiquitin Ligase Mediated by MpCRY in the Liverwort Marchantia polymorpha under Blue Light

Round 1

Reviewer 1 Report

This is a solid paper on a subject the authors have considerable experience handling.  A few minor comments:

What wavelength (or range) is meant by "blue" light?

Line 355: I recommend writing out what PPK1 is for novice readers.

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Reviewer 2 Report

I checked your manuscript and described comments below.

Superior point

1. This article suggest that COP1/SPA1 ubiquitinating HY5 is conserved in Arabidopsis.
2. The data in this paper clarified MpCRY is not an inhibitor of this process under blue light.

Minor problems.

Gene symbols etc. must be capitalized in references titles.

I wrote below about the parts that need to be fixed.

Ref. 4 and Ref. 5, cop1/spa e3 ubiquitin ligase -> CPO1/SPA3 E3 ligase

Ref. 8, e3 ubiquitin ligase cop1/spa -> E3 ubiquitin ligase COP1/SPA

Ref. 8, pap1 and pap2 ->PAP1 and PAP2

Ref.9, Cop1 -> COP1

Ref. 10, cop1 -> COP1

Ref. 11, e3 ubiquitin ligase cop1/spa -> E3 ubiquitin ligase COP1/SPA

Ref. 12, cop1 -> COP1

Ref. 13, cop1 -> COP1

Ref. 13, spa2 ->SPA2

Ref. 14, spa1 to suppress cop1 -> SPA1 to suppress COP1

Ref. 15, f-box protein fkf1 -> F-box protein FKF1

Ref. 15, cop1 -> COP1

Ref. 16, cop1 -> COP1

Ref. 17 cop1/spa -> COP1/SPA

Ref. 18, gus-cop1 -> GUS-COP1

Ref. 19, cry2 with spa1 regulates cop1 -> CRY2 with SPA1 regulates COP1

Ref. 20, spa -> SPA

Ref. 25, uvr8 -> UVR8

Ref. 27, Spa1, a wd-repeat protein -> SPA1, a WD-repeat protein

Ref. 29, cry2 -> CRY2

Ref. 30, cry2 -> CRY2

Ref. 31, hek239t -> HEK239T

Ref. 34, cop1 -> COP1

Ref. 34, spa -> SPA

Ref. 35, e3 ligases -> E3 ligases

Ref. 36, Laf1 ->LAF1

Ref. 36, cop1 -> COP1

Ref. 37, mpef1α -> MPEF1α

Ref. 37, camv35 -> CAMV35

Ref. 40, spa1 -> SPA1

Ref. 43, cry2 -> CRY2

Ref. 51, e3 ligases -> E3 ligases

Ref. 51. uv-b -> UV-B

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Reviewer 3 Report

Paper "Functions of COP1/SPA E3 ubiquitin ligase mediated by MpCRY in the liverwort Marchantia polymorpha under blue light" is very interesting.

Authors obtained Mpspa1 knockout mutants, and elaborated the function of MpCOP1/MpSPA1 complex and the effect of blue light receptor MpCRY on its function through the phenotype under blue light and specific biochemical evidence.

Quality of all Figures is good.

Paper needs statistical analysis. Authors presented large number of results but lack of testing of significant these data.

Paper needs major revision.

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Round 2

Reviewer 3 Report

Thank you for your corrections. Now, is ok.