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Article

B-Cell Epitope Mapping of the Vibrio cholera Toxins A, B, and P and an ELISA Assay

by
Salvatore G. De-Simone
1,2,3,*,
Paloma Napoleão-Pêgo
1,2,
Priscilla S. Gonçalves
1,2,3,
Guilherme C. Lechuga
1,2,
Sergian V. Cardoso
4,
David W. Provance, Jr.
1,2,
Carlos M. Morel
1 and
Flavio R. da Silva
1,2
1
Center for Technological Development in Health (CDTS)/National Institute of Science and Technology for Innovation in Neglected Diseases Populations (INCT-IDPN), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, RJ, Brazil
2
Epidemiology and Molecular Systematics Laboratory (LEMS), Oswaldo Cruz Institute, Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, RJ, Brazil
3
Program of Post-Graduation on Science and Biotechnology, Molecular and Cellular Biology Department, Biology Institute, Federal Fluminense University, Niterói 24020-036, RJ, Brazil
4
Department of Health, Graduate Program in Translational Biomedicine (BIOTRANS), University of Grande Rio (UNIGRANRIO), Caxias 25071-202, RJ, Brazil
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2023, 24(1), 531; https://doi.org/10.3390/ijms24010531
Submission received: 20 October 2022 / Revised: 20 December 2022 / Accepted: 21 December 2022 / Published: 28 December 2022
(This article belongs to the Special Issue Recent Advances of Biochemistry and Molecular Biology of Infectious Diseases)
Browse Figures
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Abstract

:
Oral immunization with the choleric toxin (CT) elicits a high level of protection against its enterotoxin activities and can control cholera in endemic settings. However, the complete B-cell epitope map of the CT that is responsible for protection remains to be clarified. A library of one-hundred, twenty-two 15-mer peptides covering the entire sequence of the three chains of the CT protein (CTP) was prepared by SPOT synthesis. The immunoreactivity of membrane-bound peptides with sera from mice vaccinated with an oral inactivated vaccine (Schankol™) allowed the mapping of continuous B-cell epitopes, topological studies, multi-antigen peptide (MAP) synthesis, and Enzyme-Linked Immunosorbent Assay (ELISA) development. Eighteen IgG epitopes were identified; eight in the CTA, three in the CTB, and seven in the protein P. Three V. cholera specific epitopes, Vc/TxA-3, Vc/TxB-11, and Vc/TxP-16, were synthesized as MAP4 and used to coat ELISA plates in order to screen immunized mouse sera. Sensitivities and specificities of 100% were obtained with the MAP4s of Vc/TxA-3 and Vc/TxB-11. The results revealed a set of peptides whose immunoreactivity reflects the immune response to vaccination. The array of peptide data can be applied to develop improved serological tests in order to detect cholera toxin exposure, as well as next generation vaccines to induce more specific antibodies against the cholera toxin.

1. Introduction

The Gram-negative bacterium Vibrio cholera causes cholera, a life-threatening diarrheal disease that is spread by ingesting contaminated food or water. This pathogen continues to be of global importance, with epidemics occurring largely in developing countries that lack sufficient infrastructure to treat sewage and provide clean water, such as Southeast Asia, Latin America, and parts of Africa. Worldwide, 1.3–4 million cases of cholera and 20,000–140,000 cholera-related deaths are recorded yearly. Approximately 50% of those affected are children aged five years or younger [1,2]. In addition, climate change and warming temperatures have spread V. cholerae to new geographical areas, highlighting the urgent need for further research to improve our understanding of this pathogen [3,4].
The strains of V. cholerae that are known to cause disease and pandemics are categorized in the O1 or O139 serogroups. Although most V. cholerae are “non-O1/O139” environmental strains, they may or may not cause some form of gastroenteritis [5,6]. The disease is highly contagious and characterized by symptoms such as the classical profuse watery diarrhea, which often leads to extreme dehydration, shock, and eventual death [6]. According to WHO reports, more than 80% of cholera patients can be successfully treated via oral rehydration therapy [7].
The complete toxin is a hexamer with an 83 kDa protein that belongs to the AB class of bacterial toxins, denoted by AB5. The single heterodimeric holotoxin A subunit (CTA) has a molecular weight of 28 kDa and is responsible for the toxicity of the bacteria, whereas the homopentameric B (five identical 11.0 kDa polypeptides) subunit (CTB5) has a molecular weight of 55 kDa. It binds the holotoxin to the eukaryotic cell receptor in order to transport the bacteria into the cell. The A subunit has a specific enzymatic function that acts intracellularly [8]. The intracellular target of CT is adenyl cyclase, which is one of the most important regulatory systems in eukaryotic cells. This enzyme mediates the transformation of ATP into cyclic AMP (cAMP), a crucial intracellular messenger in various cellular processes. In addition, CT catalyzes the transfer of ADP-ribose from NAD (Nicotinamide Adenine Dinucleotide) to a specific arginine residue in the target (Gsa) protein [8].
Consequently, this results in the activation of adenyl cyclase and, therefore, an increase in the intracellular level of cAMP which activates cAMP-dependent protein kinase. This leads to protein phosphorylation, altered ion transport, and diarrhea with massive fluid loss [9]. Therefore, public health interventions are critical in order to limit the dissemination of cholera, which can otherwise easily become epidemic, particularly in areas without proper sanitation and clean water.
Immunization with cholera vaccines is an important strategy in order to control and prevent cholera epidemics or pandemics. V. cholerae virulence factors, including CT [10,11], toxin-coregulated pili [12], lipopolysaccharide (LPS), [13] and outer membrane proteins (Omps) [14,15,16], are ideal candidates for designing a cholera vaccine. Today, two types of oral vaccines are available: attenuated oral cholera vaccines (aOCVs) and killed oral vaccines (kOCVs). The aOCVs consist of attenuated, whole V. cholerae cells that display direct efficacy in protecting populations in endemic regions [17,18]. These vaccines were recently deployed during outbreaks in non-endemic areas as part of “reactive” vaccination programs in order to block the spread of cholera [19,20]. kOCVs are effective in inducing heard protection for at least three years and are effective tools for cholera control. In addition, one dose provides short-term protection at least, which has important implications for outbreak management. However, the optimal efficacy of kOCVs requires two doses administered 14 days apart, and the vaccine needs refrigeration [21]. These features may limit the capacity of kOCVs to rapidly constrain ongoing outbreaks in destabilized or resource-limited settings. Nevertheless, single-dose live aOCVs have shown satisfactory efficacy in challenge studies [22] and early-phase clinical trials in endemic regions [23]. The design of reactive vaccination programs with a live aOCV may have the best chance of decreasing the incidence of cholera during outbreaks [24,25].
Oral cholera vaccination is a rapid approach to preventing outbreaks in at-risk settings and controlling cholera in endemic settings [26,27]. However, the benefits of vaccine-derived immunity may be short-lived due to human mobility combined with waning vaccine efficacy. As the supply and utilization of oral cholera vaccines expand, critical questions must be addressed concerning the coverage and vaccine application schedule necessary to generate herd immunity [28]. Serological diagnostic assays have the potential to be employed for large population studies [29] to provide information on the duration of the immune response, which could be improved by the identification of epitopes in CT that are recognized by the antibodies produced by a vaccine. This would advance our understandings of response continuance and herd protection.
An efficient, high-content screening method for epitope mapping involves screening peptide libraries that represent target coding sequences. By synthesizing peptides directly onto cellulose membranes, it is possible to generate refined maps of the complete epitome in viruses and the hundreds of epitopes possible in a large protein [30,31]. Commonly referred to as SPOT synthesis analysis, this strategy was applied here in order to identify the linear B-cell epitopes in three toxins from V. cholerae: enterotoxins A (8), B (3), and P (7). A total of 18 linear B-cell epitopes were identified. Three were chosen to perform peptide ELISAs and evaluated using sera from mice that were vaccinated with oral cholera. Two specifically reactive sequences were observed, further validating the systematic definition of pathogen epitomes.

2. Results

2.1. Identification of the Immunodominant IgG Epitopes in Cholera Toxin

Epitopes in the three components of CT (CTA (258aa), CTB (124aa), and CTP (221aa)) were identified based on the recognition of representative peptides. These were synthesized as a library and detected using mice antibodies immunized with an oral V. cholera vaccine (15 days post vaccination; see Section 4). The data in Figure 1A,B present the position of each peptide and the measured intensity, respectively, from the chemiluminescent detection of mouse IgG antibodies present in sera pooled from mice vaccinated with aOCV. The overlapping of the spots with signal strengths above 30% defined the minimum sequence of the epitopes. A list of the synthetic peptides and their positions on the membrane is presented in Supplementary Figure S1. The sequences constituting the reactive peptides defined 18 IgG epitopes of sizes ranging from 4 to 14 aa that were generated by vaccination with aOCV (Table 1).

2.2. Localization of the Major B Epitopes within the Three Enterotoxins

The 18 linear B epitopes identified by the SPOT synthesis analysis were distributed throughout Vc/TxA, Vc/TxB, and Vc/TxP (Figure 2). The enterotoxin chain A1 contains six epitopes (Vc/TxA-1 to Vc/TxA-6), chain A2 houses two epitopes (Vc/TxA-7 and Vc/TxA-8), enterotoxin B has three (Vc/TxB-9 to Vc/TxB-11), and seven epitopes are present in enterotoxin P (Vc/TxP-12 to Vc/TxP-18) (Table 1).
Table
Table 1. List of B-cell linear IgG epitopes identified in TxA, TxB, and P deduced from the overlap of consecutive positive peptides with signal intensities greater than 30%.
Table 1. List of B-cell linear IgG epitopes identified in TxA, TxB, and P deduced from the overlap of consecutive positive peptides with signal intensities greater than 30%.
Protein CodeaaSequence2nd Structure *CodePeptide Search **
P0155526–30ADSRPCVc/TxA-1/miGVarious bacteria
37–45SGGLMPRGQCVc/TxA-2/miGSp
66–77TQTGFVRHDDGYCVc/TxA-3/miGSp
108–115TAPNMFNVCVc/TxA-4/miGE. coli
176–180AADGYCVc/TxA-5/miGVarious bacteria
191–204AWREEPWIHHAPPGCVc/TxA-6/miGSp
244–245YQSDCVc/TxA-7/miGVarious bacteria
250–258THNRIKDELCVc/TxA-8/miGSp
P0155620–25HGTDQNCVc/TxB-9/miGSp
53–56AGKRCVc/TxB-10/miGSp
64–77KNGAIFQVEVPGSQC + S + CVc/TxB-11/miGSp
P2948517–21NECTNCVc/TxP-12/miGVarious bacteria, virus
26–40AQDPMKPERLIGTPSC + S + CVc/TxP-13/miGSp
53–59YHPAPCPCVc/TxP-14/miGSp
68–77WPHGFISSESLC + HVc/TxP-15/miGSp
90–95NDEHKTCVc/TxP-16/miGSp
112–121VIVSENVVDES + CVc/TxP-17/miGSp
174–177GITHCVc/TxP-18/miGVarious bacteria
Sp, specific epitopes; C, coil; H, helix; S, strand; * based on an I-TASSER analysis; ** UNIPROT.

2.3. Spatial Location of the Reactive Epitopes of Enterotoxin A, B, and P

The eighteen linear B epitopes identified by the SPOT synthesis analysis were distributed throughout the V. cholera toxin proteins (Figure 2). The V. cholera protein gene contains three well-defined segments: a short signal peptide in the N-terminal extension (aa 1–18), the A1 subunit chain segment (aa 19–212), the A2 subunit chain segment (aa 213–258), the B subunit (aa 1–124), and the P protein (aa 1–221).
The structures of the enterotoxin A, B, and P proteins were obtained from the protein data bank (PDB) or were predicted using AlphaFold DB to access the location of the epitopes. Alternatively, the tertiary structure was deduced using the I-TASSER server (http://zhanggroup.org/I-TASSER/, accessed on 20 March 2022). The predicted structural models of the enterotoxin A, B, and P proteins were obtained (Figure 2A–C) and displayed the possible spatial localization of the eight reactive epitopes in CTA, the three epitopes in CTB, and the seven epitopes in CTP. Most of the identified epitopes were in loop/coil structures, which were present on the protein surface and accessible to the solvent (Figure 2).

2.4. Specific and Cross-Immune IgG Epitopes

To investigate possible cross-immunity conferred by the CT proteins, the sequences of CTA, CTB, and CTP were used as templates in a multi-peptide match that searched for peptides deposited in the UniProtKB data bank. The search criteria were for four or more consecutive amino acids that were identical. This analysis suggested that twelve epitopes from V. cholerae are expected to be specific (Table 1), whereas five (Vc/TxA-1, Vc/TxA-5, Vc/TxA-7, Vc/TxP-12, Vc/TxP-18) were expected to display cross-reactivity with various bacteria. The V. cholera specific epitopes in CTA were Vc/TxA-2, Vc/TxA-3, Vc/TxA-6, and Vc/TxA-8). In CTB, the specific epitopes were Vc/TxB-9, Vc/TxB-10, and Vc/TxB-11. The epitopes Vc/TxP-13, Vc/TxP-14, Vc/TxP-15, Vc/TxP-16, and Vc/TxP-17 were determined to be specific in CTP (Table 1).

2.5. Epitope Reactivity by ELISA

The potential for serological asays based on bacterial proteins to display cross-reactivity is a well-known limitation and was a major impetus for identifying the individual linear B-cell epitopes in the cholerae enterotoxins involved in the vaccination process. We reasoned that by restricting the antigens used in an assay to capture reactive antibodies to those definitively specific to V. cholorea by sequence analysis, a diagnostic ELISA could be developed that eliminated the potential for false positive results. To that end, three epitopes were chosen: one from the major antigenic region of chain A (TQTGFVRHDDGYGGG; Vc/TxA-3), one from chain B (KNGAIFQVEVPGSQG; Vc/TxB-11), and one from the protein P (WPHGFISSESLGGGG; Vc/TxP-15). These epitopes were chosen to generate multi-antigen peptides (MAPs) by solid-phase synthesis using the F-moc strategy. The schematic structures of the dendrimers are shown in Figure 3.
The sera from each immunized mouse reacted with all three synthetic peptides that were derived from V. cholera without reacting to the negative control peptide QEVRKYFCV (Vaccinia virus; Figure 1A G9, G17). Importantly, no reactivity was observed with the sera from non-vaccinated healthy animals. These results are shown in Figure 4A and were calculated to be significant (p < 0.001).
Based on a Receiver Operating Characteristics (ROC) curve analysis (Figure 4B), the area under the curve (AUC) for the epitope Vc/TxB-11 was 0.9987 (p < 0.0001), as detected by ELISA with a confidence interval of 95%, demonstrating high diagnostic accuracy for the peptide. The Vc/TxB-A3 peptide presented a reactivity lower than the peptide Vc/TxB-11 epitope, but both demonstrated 100% sensitivity and specificity. The values of the ROC curve were determined as the reactivity to Vc/TxP-15 peptide was much weaker.

3. Discussion

Vaccine production is usually a laborious and costly experimental process. Nevertheless, these immunobiological molecules are the most potent countermeasures used to prevent infectious diseases. Today, two different types of cholera vaccines exist that are commercially available or under development: inactivated vaccines, such as Dukoral® (Crucell, Netherlands, 1990, denatured vCh-), mORCAX™ (VaBiotech, Hoan Kiem District, Hanoi, Vietnam) and Sanchol™ (Shantha, Biotechnics-Sanofi Pasteur, Muppiriddipalli, Telangana, India), Euvichol or EuvicholPlus (EuBiologics Co., Seoul, Republic of Soul Korea), and Dukoral (Valneva, Kirkland, QC, Canada), and live attenuated vaccines, such as CVD103-HgR and Peru-15 or CholeraGarde and Vaxchora (Emergent BioSolutions, Rockville, MD, USA) [29,32,33]. Although it has been proven that both types are protective for cholera, the immunological mechanisms dependent on B-cell and/or T-cell immunity are unknown [29]. It is recognized that the most important stimulators of innate immunity and the subsequent adaptive immune response are the LPS O antigen and CT [6,10], which activate the NF-κB and IL-1 systems which are critical factors for promoting long-term mucosal protection [8,13,34,35]. Thus, perhaps the most important question remains how to create more efficacious cholera vaccines, as current vaccine efficacies are only ~60% [27,36]. Therefore, the identification of immunodominant epitopes that interact with the antibodies generated in response to vaccination can contribute to the understanding and selection of better antigens.
In this work, we experimentally identified all the linear B epitopes in the CT recognized by IgG antibodies from mice vaccinated with the inactivated Sanchol™ vaccine using a peptide microarray analysis. CT is the primary virulence factor of V. cholerae, and ingestion of as little as 5 g produces the symptoms of cholera [37]. The reactivity of the serum from vaccinated animals against a subset of regions in CT showed that the vaccine has a high antigenic potential with only a single dose. Eight B linear epitopes were readily identified in CTA, three in CTB, and seven in protein CTP. All eighteen epitopes were exposed on the molecular surface and were accessible to the immune system (Figure 3).
The CTA subunit is synthesized as a single polypeptide with a signal peptide (aa 1–18). Enzymatic activity is initiated after proteolytic cleavage, “nicking” at residue Arg-192, which produces two functional domains: A1 (~21.8 kDa, aa 19–204) and A2 (~5.4 kDa, aa 206–258). The two fragments remain covalently associated by a disulfide bond. The B subunit contains five chains that form a pentameric ring around a central pore structure. CTA1 is responsible for ADP-ribosyltransferase activity, and the alpha-helical CTA2 has an α–helix tail that moors the CTA1 and CTB5 subunits together [38,39,40,41]. CTB is a non-toxic subunit that produces target cells that block antibodies and is a potent mucosal immunostimulatory adjuvant that can incite potent mucosal and immune system responses [42]. Because of the strong adjuvanticity of CTB, it is applied in different peptide and DNA vaccines, such as microbial and cancer vaccines [43,44]. In addition to playing a role as an immunostimulatory adjuvant, CTB is also a powerful protective antigen.
CTA and CTB seem to have the greatest protective importance of the three chains that constitute the cholera toxin. Initially, CTA was not considered to have immunological importance, despite having the toxic portion of the molecule [34]. However, this assumption has been revised due to recent studies that showed that anti-CTA antibodies are capable of toxin neutralization at very low concentrations in persons living in areas where cholera is endemic [9]. This ability has been attributed to the cross-reactivity of CTA epitopes with the enterotoxigenic Escherichia coli (ETEC) heat-labile toxin (LT) [45] together with the frequent exposure of people living in endemic areas to ETEC LT toxin [46]. Our study mapped three epitopes in CTA that present an extensive potential to cross-react with bacterial toxins (Table 1), more so than the other subunits.
Studies describing the identification of CT epitopes are rare in the literature. Nevertheless, previous cross-reaction studies using polyclonal rabbit sera and B-toxin synthetic peptides neutralization have identified the presence of three immunogenic peptides, aa 8–20, aa 50–64 (anti-CP3) [47], and aa 69–85, in the CTB [48]. However, our studies also identified similar immunogenic peptides using sera from mice that were orally vaccinated (Table 1). They were identified as Vc/TxB-9miG (aa 20–25), Vc/TxB-10/miG (aa 53–56), and Vc/TxB-11/miG (aa 64–77). In another study in mice, it was demonstrated that the anti-CTB specific immune response was dominant and protective [49]. Yet, circulating levels of CTB-IgG antibodies and CTB-specific memory B-cells wane within months after natural infection [50,51], as well as in human challenge studies [42,52]. Together, CTB provides only a short-term immunity boost [49]. More concerningly, overall protection with the CTB-containing vaccine appeared to drop to lower levels than the non-CTB-containing vaccine within two years after vaccination [42]. Therefore, the three epitopes (Vc/TxB-9/miG, Vc/TxB-10/miG, and Vc/TxB-11/miG) identified in the B subunit should be of interest for vaccine development despite descriptions against lasting protection conferred by the B subunit. Concerning CTP, no detailed studies on its immunogenic importance were found. However, six of the seven identified epitopes are unique and can potentially be used in developing chimeric polyproteins for diagnostic purposes.
Concerning serological diagnoses, the ELISA results using the MAP4-epitope peptides revealed that two of the peptide (Vc/TxA-3 and Vc/TxB-11) epitopes appropriately discriminate between negative and positive samples (p < 0.0001). The peptide TxP-15 presented a low immune response 15 days after vaccination; however, their response index increased 30 days post vaccination (Figure 4). The weak immune response induced by the entire peptide P has been characterized [53]. Although they reflect the mouse immune response, these results are important since these epitopes are eligible for phase IIA studies, which involves an estimate of their accuracy (sensitivity and specificity) for the index test in discriminating between diseased and non-diseased people in a clinically relevant population [54]. The performance of the selected epitopes strongly supports the continued use of these epitopes within a chimeric multi-epitope protein [55] as a target in more sensitive and fast diagnostic tests [56].

4. Materials and Methods

4.1. Immunization of Mice

Thirty Balb C mice (15–21 g) were immunized orally with 20 µL of whole attenuated SchancolTM cholera vaccine (lot SCN021A15) produced by Shantha Biotechnics Ltd. (Muppiriddipalli, Telangana, India). Fifteen days after vaccination, blood samples were obtained, followed by a full bleed at 30 days. For the unvaccinated controls, blood samples were collected from 30 healthy mice. Each group of serum was collected separately, distributed in aliquots of 0.5 mL in Eppendorf tubes, and stored at −20 °C.

4.2. Synthesis of the Cellulose Membrane-Bound Peptide Array

A library of one hundred, twenty-two peptides that spanned the entire coding sequence of cholera enterotoxin A (P01555, 258 aa), enterotoxin B (P01556, 124 aa), and Toxin P (P29485, 221 aa) of serotype O1 was synthesized onto a cellulose membrane according to standard SPOT synthesis protocols using an Auto-Spot Robot ASP-222 (Intavis Bioanalytical Instruments AG, Köln, Germany) [57]. Each peptide contained 15 amino acids of coding sequence that overlapped the previous and next peptide by 10 residues. To optimize the library, a GSGSG spacer sequence was inserted at the amino and carboxy termini of each protein (points A1, D5, D6, C4, C5, and F2). Library construction and program execution were conducted with the MultiPep program (Intavis). The peptide QEVRKYFCV (Vaccinia virus, spots G9 and G17) was included as a negative control, and the peptides KEVPALTAVETGATN (Poliovirus, spots G3 and G11), GYPKDGN AFNNLDRI (Clostridium tetani, spots G5 and G13), and YDYDVP DYA GYPYDV (hemagglutinin of Influenza virus, spots G7, G15, and G24) were included as positive controls.
Coupling reactions were followed a blocking step by acetylation with acetic anhydride (4%, v/v) in N, N-dimethylformamide. Next, the fluorenylmethyloxycarbonyl (F-moc) protecting group was removed from the N-terminus of the peptide by adding a 20% solution of piperidine in DMF. This cycle of coupling, blocking, and deprotection was repeated in order to add each successive amino acid until the desired peptide was generated. After adding the last amino acid, the side chains of the amino acids were deprotected using a solution of dichloromethane-trifluoroacetic acid-triisopropylsilane (1:1:0.05, v/v/v) and washed with ethanol as described previously [58]. Membranes containing the synthetic peptides were probed immediately.

4.3. Screening of SPOT Membranes

The SPOT membranes were washed for 10 min with TBS-T (50 mM Tris, 136 mM NaCl, 2 mM KCl, and 0.05% Tween® 20, pH 7.4) and then blocked with TBS-T containing 1.5% BSA for 90 min at 8 °C under agitation. After extensive washing with TBS-T, the membranes were incubated for 12 h with a pool (n = 10) of vaccinated mouse sera diluted (1:150 for IgG detection) in TBS-T with 0.75% BSA and then washed again with TBS-T. After this, the membranes were incubated with sheep anti-mouse IgG alkaline phosphatase (diluted 1:5000), prepared in TBS-T with 0.75% BSA for 1 h, and then washed with TBS-T and CBS (50 mM citrate-buffered saline). Finally, the chemiluminescent substrate Nitro-Block II was added to complete the reaction. The integrity of the library synthesis was confirmed with the reactivity of the control peptides to human sera.

4.4. Scanning and Measurement of Spot Signal Intensities

Chemiluminescent signals were detected on an Odyssey FC (LI-COR Bioscience, Lincoln, NE, USA) using the same conditions described previously [59] with minor modifications. Briefly, a digital image file was generated at a resolution of 5 MP, and the signal intensities were quantified using the TotalLab TL100 (v 2009, Nonlinear Dynamics, Newcastle-Upon-Tyne, UK) software using its automated grid search for 384 spots. The signal intensities (SI) were exported to Excel (Microsoft Corp., Redmond, WA, USA) in order to remove background signals (signal at the negative control) and normalized to a percent of the highest signal measured. An epitope was defined in the sequences of two or three positive contiguous spots that presented a normalized SI greater than or equal to 30% [60]. For three or more contiguous spots ≥ 30%, epitopes were identified visually when three or more contiguous spots were positive.

4.5. Preparation of the Multi-Antigen Peptides

For the preparation of the dendrimer multi-antigen peptides (MAPs), a solid phase synthesis protocol was performed with tetrameric (F-moc) 4-Lys2-Lys-β-Ala Wang resin (CEM Corp, Charlotte, NC, USA) as previously described [59]. The peptides possessed the sequence: (A) Vc/TxA-3 (TQTGFV RHDDGYGGG), (B) Vc/TxB-11 (KNGAIFQVEVP GSQG), and (C) Vc/TxP-15 (WPHGFISSESLGGGG). The constructs were prepared in an automated peptide synthesizer (MultiPep-1, CEM Corp, Charlotte, NC, USA), and the side chains of tetrafunctional F-moc amino acids were protected with TFA-labile protecting groups as required. Once sequence assembly was completed, the F-moc groups were removed, and the peptide resin was cleaved and fully deprotected with TFA/H2O/EDT/TIS (94/2.5/2.5/1.0 v/v, 90 min). The peptides were precipitated by adding chilled diethyl ether, centrifuged (30,000× g, 10 min at 4 °C), and the pellet was dissolved in aqueous AcOH (10% v/v), dried, and stored as a lyophilized powder. When necessary, the MAP was dissolved in water, centrifuged (10,000× g, 60 min at 15 °C), and the supernatant was filtered by a Centricon™ (Merck Millipore, Burlington, MA, USA) ten filter. Purification of the MAPs was performed at BIOMANGUINHOS-FIOCRUZ, Rio de Janeiro, Brazil. An XBridge BEH C18 (2.7 μ, 5 cm × 4.6 mm) column coupled to a water autopurify HPLC system (Water Corporation, Newcastle, NS, Australia) at a flow rate of 1.2 mL min−1 using mobile phases A [0.05% formic acid in water (18 MΩ × cm)] and B [(0.05% formic acid in ACN (acetonitrile acid)] (v/v) in water and a gradient of 0–97 B in 40 min. Detection at 200–300 nm using a diode array was used during purification.
For ESI-TOF, the peptides were solubilized in deionized water to a final concentration of 10 µg/mL and then formic acid was added to a final concentration of 0.1%. The mass spectrometer used was the Water UPLC model Acquity-I Class (Water Corp., Newcastle, Australia), and the samples were injected at 1 µL/min. The range used for ion detection was from 1000 to 11,500 m/z.

4.6. ELISA

Peptide-based ELISA assays were performed as described previously [60]. Briefly, wells of 4HB NUNC plates for ELISAs were coated with 50 ng of each peptide in 100 µL of coating buffer (Na2CO3–NaHCO3, pH 9.6) overnight at 4 °C. Next, the plates were washed three times using PBS-T washing buffer (PBS with 0.1% Tween® 20, pH 7.2) and blocked using PBS-T with 2.5% BSA over 2 h at 37 °C. Next, diluted mouse sera (100 µL) in coating buffer was applied and incubated for 2 h at 37 °C. Following several washes with PBS-T, the plates were incubated for 2 h at 37 °C with 100 µL of goat anti-mouse IgG conjugated to alkaline phosphatase (Sigma, St Louis, MO, USA) diluted in coating buffer, washed, and exposed to PNPP substrate (Sigma, St Louis, MO, USA). Absorbance was measured at 405 nm on a FlexStation 3 Microplate Reader (Molecular Devices, Sunnyvale, CA, USA). Initially, the optimal serum dilution value of 1:100 was determined by a titration series of vaccinated mouse sera and by constructing the receiver operating characteristic (ROC) curve.

4.7. Structural Localization of the IgG Epitopes and Bioinformatics Tools

To ascertain the location of the epitope within the 3D molecular structure of enterotoxin A, enterotoxin B, and toxin coregulated pilus biosynthesis protein P (Toxin P) from V. cholerae, we constructed in silico protein models using the I-TASSER server (http://zhanglab.ccmb, accessed on 25 March 2022). Models were chosen according to best C-score and TM-score (topological evaluation value) [61]. The resulting 3D structural models were also analyzed in the AlphaFold database [62]. Data bank searches for V. cholerae sequence homologies were performed using previously identified sequences and annotated proteins in other organisms in the database UniProt (http://www.uniprot.org/, accessed on 20 April 2022) and multiple peptide match (https://research.bioinformatics.udel.edu/peptide match/index.jsp, accessed on 10 May 2022).

4.8. Statistical Analysis

Data were analyzed using the program R (version 3.6.0) and R Studio. The paired t-test was applied in order to compare the statistical significance between the two samples. Statistical significance was considered at p ≤ 0.05. GraphPad Prism version 5.0 was used to analyze the receiver operating characteristic curve (ROC).

5. Conclusions

A high-throughput immune response profile using a peptide array that was directly generated on a cellulose membrane facilitated the identification of the major antigenic determinants in enterotoxins A, B, and P, which were recognized by antibodies from mice that were orally vaccinated with a single dose of the aOCV. Eighteen IgG epitopes that were distributed throughout the bacterial CT proteins were identified. Six epitopes were identified in the A1 chain of the enterotoxin, two in the A2 chain, three in enterotoxin B, and seven in enterotoxin P. Six of the epitopes showed a level of similarity to the proteins in other pathogens that suggested a high potential for cross-reactivity. Twelve V. cholera specific epitopes were defined, and their spatial localizations within protein structural models were revealed using bioinformatics. The performance of three of these epitopes (Vc/TxA-3, Vc/TxB-11, and Vc/TxP-15) to detect antibodies generated in mice as a response to vaccination was empirically confirmed by an indirect ELISA. The molecular characterization of linear B-cell IgG epitopes in cholera enterotoxins can be used to construct a polyepitope chimeric protein for use in next generation rapid diagnostic tests and highlights the utility of epitope mapping to better understand the immune response to current and future vaccines in order to improve the production of specific neutralizing antibodies.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/ijms24010531/s1.

Author Contributions

Conceptualization, S.G.D.-S. and F.R.d.S.; methodology, P.N.-P., F.R.d.S., P.S.G., S.V.C. and G.C.L.; validation, S.G.D.-S. and C.M.M.; writing—original draft, S.G.D.-S.; writing—review and editing, D.W.P.J. and S.G.D.-S.; funding acquisition, S.G.D.-S. and C.M.M. All authors have read and agreed to the published version of the manuscript.

Funding

This work was supported by the Brazilian Council for Scientific Research (CNPq #30515-2020-5) and Carlos Chagas Filho Foundation of Research Support of the State of Rio de Janeiro (FAPERJ #200.960-2022). This support was given to S.G.D.-S. Funding was also provided by FAPERJ (#210.003/2018) through the National Institutes of Science and Technology Program (INCT) to C.M.M. (INCT-IDPN).

Institutional Review Board Statement

The study was approved by the UNIGRANRIO (CEUA 052/2021) study center ethics committee and conducted under good clinical practice and all applicable regulatory requirements, including the Declaration of Helsinki.

Informed Consent Statement

Not applicable.

Data Availability Statement

The data presented in this study are available on request from the corresponding author.

Acknowledgments

We thank the National Institute of Quality Control (INCQs) from FIOCRUZ for their support with the vaccine and with animal immunization, and the Ulisses Cesar de Araujo from the Multiuser Research Laboratory in Mass Spectrometry applied to Biomolecules (MultiMasses/IBRAG/UERJ). P.N.-P. and G.C.L. are Postdocs fellow from CAPES-FIOCRUZ program. P.S.G. is a CAPES fellow from the Post-Graduation Program on Science and Biotechnology, Federal Fluminense University.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Ali, M.; Nelson, A.R.; Lopez, A.L.; Sack, D.A. Updated global burden of cholera in endemic countries. PLoS Negl. Trop. Dis. 2015, 9, e0003832. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  2. Yuki, Y.; Nojima, M.; Hosono, O.; Tanaka, H.; Kimura, Y.; Satoh, T.; Imoto, S.; Uematsu, S.; Kurokawa, S.; Kashima, K.; et al. Oral MucoRice-CTB vaccine for safety and microbiota-dependent immunogenicity in humans: A phase 1 randomized trial. Lancet Microbe 2021, 2, e429–e440. [Google Scholar] [CrossRef] [PubMed]
  3. Vezzulli, L.; Grande, C.; Reid, P.C.; Hélaouët, P.; Edwards, M.; Höfle, M.G.; Brettar, I.; Colwell, R.R.; Pruzzo, C. Climate influence on Vibrio and associated human diseases during the past half-century in the coastal North Atlantic. Proc. Natl. Acad. Sci. USA 2016, 113, E5062–E5071. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  4. Deeb, R.; Tufford, D.; Scott, G.I.; Moore, J.G.; Dow, K. Impact of climate change on Vibrio vulnificus abundance and exposure risk. Estuaries Coasts 2018, 41, 2289–2303. [Google Scholar] [CrossRef] [PubMed]
  5. Baker-Austin, C.; Oliver, J.D.; Alam, M.; Ali, A.; Waldor, M.K.; Qadri, F.; Martinez-Urtaza, J.; Martinez-Urtaza, J. Vibrio spp. Infections. Nat. Rev. Dis. Prim. 2018, 4, 1–19. [Google Scholar] [CrossRef]
  6. Legros, D.; Partners of the Global Task Force on Cholera Control. Global cholera epidemiology: Opportunities to reduce the burden of cholera by 2030. J. Infect. Dis. 2018, 218, S137–S140, Erratum in J. Infect. Dis. 2019, 219, 509. [Google Scholar] [CrossRef]
  7. WHO. Cholera; World Health Organization: Geneva, Switzerland, 2022. [Google Scholar]
  8. Banerjee, T.; Grabon, A.; Taylor, M.; Teter, K. cAMP-Independent activation of the unfolded protein response by cholera toxin. Infect. Immun. 2021, 89, e00447-e20. [Google Scholar] [CrossRef]
  9. Clemens, J.D.; Nair, G.B.; Ahmed, T.; Qadri, F.; Holmgren, J. Cholera. Lancet 2017, 390, 1539–1549. [Google Scholar] [CrossRef]
  10. Bourque, D.L.; Bhuiyan, T.R.; Genereux, D.P.; Rashu, R.; Ellis, C.N.; Chowdhury, F.; Khan, A.I.; Alam, N.H.; Paul, A.; Hossain, L.; et al. Analysis of the human mucosal response to cholera reveals sustained activation of innate immune signaling pathways. Infect. Immun. 2018, 86, e00594-17. [Google Scholar] [CrossRef] [Green Version]
  11. Sánchez, J.; Holmgren, J. Cholera toxin-a foe & a friend. Indian J. Med. Res. 2011, 133, 153. [Google Scholar]
  12. Taylor, R.K.; Miller, V.L.; Furlong, D.B.; Mekalanos, J.J. Use of phoA gene fusions to identify a pilus colonization factor coordinately regulated with cholera toxin. Proc. Natl. Acad. Sci. USA 1987, 84, 2833–2837. [Google Scholar] [CrossRef] [PubMed]
  13. Waldor, M.K.; Colwell, R.; Mekalanos, J.J. The Vibrio cholerae O139 serogroup antigen includes an O-antigen capsule and lipopolysaccharide virulence determinants. Proc. Natl. Acad. Sci. USA 1994, 91, 11388–11392. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  14. Sperandio, V.; Giron, J.A.; Silveira, W.D.; Kaper, J.B. The OmpU outer membrane protein, a potential adherence factor of Vibrio cholerae. Infect. Immun. 1995, 63, 4433–4438. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  15. Sengupta, D.K.; Sengupta, T.K.; Ghose, A.C. Major outer membrane proteins of Vibrio cholerae and their role in induction of protective immunity through inhibition of intestinal colonization. Infect. Immun. 1992, 60, 4848–4855. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  16. Nandi, B.; Nandy, R.K.; Sarkar, A.; Ghose, A.C. Structural features, properties and regulation of the outer-membrane protein W (OmpW) of Vibrio cholerae. Microbiology 2005, 151, 2975–2986. [Google Scholar] [CrossRef] [Green Version]
  17. Qadri, F.; Ali, M.; Chowdhury, F.; Khan, A.I.; Saha, A.; Khan, I.A.; Begum, Y.A.; Bhuiyan, T.R.; Chowdhury, M.I.; Uddin, M.J.; et al. Feasibility and effectiveness of oral cholera vaccine in an urban endemic setting in Bangladesh: A cluster randomized open-label trial. Lancet 2015, 386, 1362–1371. [Google Scholar] [CrossRef] [PubMed]
  18. Bi, Q.; Ferreras, E.; Pezzoli, L.; Legros, D.; Ivers, L.C.; Date, K.; Qadri, F.; Digilio, L.; Sack, D.A.; Ali, M.; et al. Protection against cholera from killed whole-cell oral cholera vaccines: A systematic review and meta-analysis. Oral cholera vaccine working group of the global task force on cholera control. Lancet Infect. Dis. 2017, 17, 1080–1088. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  19. Peak, C.M.; Reilly, A.L.; Azman, A.S.; Buckee, C.O. Prolonging herd immunity to cholera via vaccination: Accounting for human mobility and waning vaccine effects. PLoS Negl. Trop. Dis. 2018, 12, e0006257. [Google Scholar] [CrossRef] [Green Version]
  20. Royal, J.M.; Reeves, M.A.; Matoba, N. Repeated oral administration of a KDEL-tagged recombinant cholera toxin B subunit effectively mitigates DSS colitis despite a robust immunogenic response. Toxins 2019, 11, 678. [Google Scholar] [CrossRef] [Green Version]
  21. Kabir, S. Critical analysis of compositions and protective efficacies of oral killed cholera vaccines. Clin. Vaccine Immunol. 2014, 21, 1195–1205. [Google Scholar] [CrossRef]
  22. Chen, W.H.; Cohen, M.B.; Kirkpatrick, B.D.; Brady, R.C.; Galloway, D.; Gurwith, M.; Hall, R.H.; Kessler, R.A.; Lock, M.; Haney, D.; et al. Single-dose live oral cholera vaccine CVD 103-HgR protects against human experimental infection with Vibrio cholerae El Tor. Clin. Infect. Dis. 2016, 62, 1329–1335. [Google Scholar] [CrossRef] [PubMed]
  23. Holmgren, J.J. Modern history of cholera vaccines and the pivotal role of ICDDR. Infect. Dis. 2021, 224, S742–S748. [Google Scholar] [CrossRef] [PubMed]
  24. Wierzba, T.F. Oral cholera vaccines and their impact on the global burden of disease. Hum. Vaccines Immunother. 2019, 15, 1294–1301. [Google Scholar] [CrossRef] [Green Version]
  25. Qadri, F.; Chowdhury, M.I.; Faruque, S.M.; Salam, M.A.; Ahmed, T.; Begum, Y.A.; Saha, A.; Al Tarique, A.; Seidlein, L.V.; Park, E.; et al. PXV Study Group, Peru-15, a live attenuated oral cholera vaccine, is safe and immunogenic in Bangladeshi toddlers and infants. Vaccine 2007, 25, 231–238. [Google Scholar] [CrossRef] [PubMed]
  26. Calain, P.; Chaine, J.P.; Johnson, E.; Hawley, M.L.; O’Leary, M.J.; Oshitani, H.; Chaignat, C.L. Can oral cholera vaccination play a role in controlling a cholera outbreak? Vaccine 2004, 22, 2444–2451. [Google Scholar] [CrossRef] [PubMed]
  27. Song, K.R.; Lim, J.K.; Park, S.E.; Saluja, T.; Cho, S.I.; Wartel, T.A.; Lynch, J. Oral cholera vaccine efficacy and effectiveness. Vaccines 2021, 9, 1482. [Google Scholar] [CrossRef]
  28. Khatib, A.M.; Ali, M.; von Seidlein, L.; Kim, D.R.; Hashim, R.; Reyburn, R.; Ley, B.; Thriemer, K.; Enwere, G.; Hutubessy, R.; et al. Effectiveness of an oral cholera vaccine in Zanzibar: Findings from a mass vaccination campaign and observational cohort study. Lancet Infect. Dis. 2012, 1, 837–844. [Google Scholar] [CrossRef]
  29. Kanungo, S.; Azman, A.S.; Ramamurthy, T.; Deen, J.; Dutta, S. Cholera. Lancet 2022, 399, 1429–1440. [Google Scholar] [CrossRef]
  30. Ali, M.; Sur, D.; You, Y.A.; Kanungo, S.; Sah, B.; Manna, B.; Puri, M.; Wierzba, T.F.; Donner, A.; Nair, G.B.; et al. Herd protection by a bivalent killed whole-cell oral cholera vaccine in the slums of Kolkata, India. Clin. Infect. Dis. 2013, 56, 1123–1131. [Google Scholar] [CrossRef] [Green Version]
  31. De Groot, A.S.; Moise, L.; McMurry, J.A.; Martin, W. Epitope-based immunome-derived vaccines: A strategy for improved design and safety. In Clinical Applications of Immunomics; Springer: Heidelberg, Germany, 2009; pp. 39–69. [Google Scholar]
  32. Winkler, D.F.H. SPOT Synthesis: The solid-phase peptide synthesis on planar surfaces. Methods Mol. Biol. 2020, 2103, 151–173. [Google Scholar]
  33. Pastor, M.; Pedraz, J.; Esquisabel, A. The state-of-the-art of approved and under-development cholera vaccines. Vaccine 2013, 31, 4069–4078. [Google Scholar] [CrossRef] [PubMed]
  34. Chiappelli, F.; Khakshooy, A.; Balenton, N. Clinical immunology of Cholera—Current trends and directions for future advancement. Bioinformation 2017, 13, 352–355. [Google Scholar] [CrossRef] [PubMed]
  35. Holmgren, J. An update on cholera immunity and current and future cholera vaccines. Trop. Med. Infect. Dis. 2021, 6, 64. [Google Scholar] [CrossRef]
  36. Shaikh, H.; Lynch, J.; Kim, J.; Excler, J.L. Current and future cholera vaccines. Vaccine 2020, 38, A118–A126. [Google Scholar] [CrossRef]
  37. Pinkhasov, J.; Alvarez, M.L.; Pathangey, L.B.; Tinder, T.L.; Mason, H.S.; Walmsley, A.M.; Gendler, S.J.; Mukherjee, P. Analysis of a cholera toxin B subunit (CTB) and human mucin 1 (MUC1) conjugate protein in a MUC1-tolerant mouse model. Cancer Immunol. Immunother. 2010, 59, 1801–1811. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  38. Mekalanos, J.; Collier, R.; Romig, W. Enzymic activity of cholera toxin. II. Relationships to proteolytic processing, disulfide bond reduction, and subunit composition. J. Biol. Chem. 1979, 254, 5855–5861. [Google Scholar] [CrossRef] [PubMed]
  39. Sánchez, J.; Holmgren, J. Cholera toxin structure, gene regulation, and pathophysiological and immunological aspects. Cell. Mol. Life Sci. 2008, 65, 1347–1360. [Google Scholar] [CrossRef] [PubMed]
  40. Bharati, K.; Ganguly, N.K. Cholera toxin: A paradigm of a multifunctional protein. Indian J. Med. Res. 2011, 133, 179. [Google Scholar]
  41. Sikora, A.E. Proteins secreted via the type II secretion system: Smart strategies of Vibrio cholerae to maintain fitness in different ecological niches. PLoS Pathog. 2013, 9, e1003126. [Google Scholar] [CrossRef]
  42. Mayo, S.; Royo, F.; Hau, J. Correlation between adjuvanticity and immunogenicity of cholera toxin B subunit in orally immunized young chickens. APMIS 2005, 113, 284–287. [Google Scholar] [CrossRef]
  43. Kim, M.S.; Yi, E.J.; Kim, Y.I.; Kim, S.H.; Jung, Y.S.; Kim, S.R.; Iwawaki, T.; Ko, H.J.; Chang, S.Y. ERdj5 in innate immune cells is a crucial factor for the mucosal adjuvanticity of cholera toxin. Front. Immunol. 2019, 10, 1249. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  44. Hou, J.; Liu, Y.; Tao, R.; Hsi, J.; Shao, Y.; Wang, H. Cholera Toxin B subunit acts as a potent systemic adjuvant for HIV-1 DNA vaccination intramuscularly in mice. Hum. Vaccines Immunother. 2014, 10, 1274–1283. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  45. Price, G.A.; Holmes, R.K. Evaluation of TcpF-A2-CTB chimera and evidence of additive protective efficacy of immunizing with TcpF and CTB in the suckling mouse model of cholera. PLoS ONE 2012, 7, e42434. [Google Scholar] [CrossRef] [PubMed]
  46. Zareitaher, T.; Sadat, T.; Seyed, A.S.; Gargari, L.M. Immunogenic efficacy of DNA and protein-based vaccine from a chimeric gene consisting OmpW, TcpA and CtxB, of Vibrio cholerae. Immunobiology 2022, 227, 152190. [Google Scholar] [CrossRef]
  47. Jacob, C.O.; Harari, I.; Arnon, R.; Sela, M. Antibodies to cholera toxin synthetic peptides of increasing size and their reactivity with related toxins. Vaccine 1986, 4, 95–98. [Google Scholar] [CrossRef]
  48. Jacob, C.O.; Sela, M.; Arnon, R. Antibodies against synthetic peptides of the B subunit of cholera toxin: Cross-reaction and neutralization of the toxin. Proc. Natl. Acad. Sci. USA 1983, 80, 7611–7615. [Google Scholar] [CrossRef] [Green Version]
  49. Sela, M.; Arnon, R.; Jacob, C.O. Synthetic peptides with antigenic specificity for bacterial toxins. In Ciba Foundation Symposium 119-Synthetic Peptides as Antigens: Synthetic Peptides as Antigens; John Wiley & Sons, Ltd.: Chichester, UK, 1986; Volume 119, pp. 184–199. [Google Scholar] [CrossRef]
  50. Provenzano, D.; Kovác, P.; Wade, W.F. The ABCS (antibody, B cells, and carbohydrate epitopes) of cholera immunity: Considerations for an improved vaccine. Microbiol. Immunol. 2006, 50, 899–927. [Google Scholar] [CrossRef]
  51. Ali, M.; Lopez, A.L.; You, Y.A.; Kim, Y.E.; Sah, B.; Maskery, B.; Clemens, J. The global burden of cholera. Bull. WHO 2012, 90, 209–218. [Google Scholar] [CrossRef]
  52. Jeon, S.; Kelly, M.; Yun, J.; Lee, B.; Park, M.; Whang, Y.; Lee, C.; Halvorsen, Y.D.; Verma, S.; Charles, R.C.; et al. Scalable production and immunogenicity of a cholera conjugate vaccine. Vaccine 2021, 39, 6936–6946. [Google Scholar] [CrossRef]
  53. Leung, D.T.; Chowdhury, F.C.; Calderwood, S.B.; Qadri, F.; Ryan, E.T. Immune responses to cholera in children. Expert Rev. Anti-Infect. Ther. 2012, 4, 435–444. [Google Scholar] [CrossRef] [Green Version]
  54. Glenny, A.T.; Hopkins, B.E. Diphtheria toxoid as an immunizing agent. Br. J. Exp. Pathol. 1923, 4, 283. [Google Scholar]
  55. Napoleão-Pêgo, P.; Carneiro, F.R.G.; Durans, A.M.; Gomes, L.R.; Morel, C.M.; Provance, D.W., Jr.; De-Simone, S.G. Performance assessment of multi-epitope chimeric antigen for serodiagnosis of acute phase of Mayaro fever. Sci. Rep. 2021, 11, 15374. [Google Scholar] [CrossRef]
  56. Colli, A.; Fraquelli, M.; Casazza, G.; Conte, D.; Nikolova, D.; Duca, P.; Thorlund, K.; Gluud, C. The Architecture of diagnostic research: From bench to bedside-research guidelines using liver stiffness as an example. Hepatology 2014, 60, 408–418. [Google Scholar] [CrossRef]
  57. De Simone, S.G.; Pêgo, P.N.; De-Simone, T.S. Spot synthesis: A healthy and sensitive peptide microarray assay to detect IgE antibodies. In Peptide Microarrays: Methods and Protocols, 2nd ed.; Cretich, M., Chiari, M., Eds.; Methods in Molecular Biology; Human Press: New York, NY, USA, 2016; Volume 1352, pp. 263–277. [Google Scholar]
  58. De-Simone, S.G.; Gomes, L.R.; Napoleão-Pêgo, P.; Lechuga, G.C.; Pina, J.C.; Silva, F.R. Identification of linear B epitopes liable for the protective immunity of diphtheria toxin. Vaccines 2021, 9, 313. [Google Scholar] [CrossRef]
  59. Silva, F.R.; Napoleão-Pêgo, P.; De-Simone, S.G. Identification of linear B epitopes of pertactin of Bordetella pertussis induced by immunization with whole and acellular vaccine. Vaccine 2014, 32, 6251–6258. [Google Scholar] [CrossRef] [Green Version]
  60. De-Simone, S.; Souza, A.L.A.; Melgarejo, A.R.; Aguiar, A.S.; Provance, D.W., Jr. Development of elisa assay to detect specific human IgE anti-therapeutic horse sera. Toxicon 2017, 138, 37–42. [Google Scholar] [CrossRef] [PubMed]
  61. Roy, A.; Kucukural, A.; Zhang, Y. I-TASSER: A unified platform for automated protein structure and function prediction. Nat. Protoc. 2010, 5, 725–738. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  62. Jumper, J.; Evansm, R.; Pritzel, A.; Green, T.; Figurnov, M.; Ronneberger, O.; Tunyasuvunakool, K.; Bates, R.; Žídek, A.; Potapenko, A.; et al. Highly accurate protein structure prediction with AlphaFold. Nature 2021, 596, 583–589. [Google Scholar] [CrossRef]
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Figure 1. Fine epitope mapping of Vibrio cholerae toxin A (P01555), B (P01556), and P (P29485). A membrane-bound peptide library representing the three chains of the toxin was probed with a pool of vaccinated mouse sera (n = 15), and reactivity was detected using goat anti-mouse IgG alkaline phosphatase alkaline labeled secondary antibody and chemiluminescence substrate. Panel (A) presents an image of the peptide array showing reactivity as dark circles. The panels in (B) graphically present the percent signal after normalizing the signals to the positive and negative controls. The sequences of peptides in each position are listed in Supplementary Figure S1.
Figure 1. Fine epitope mapping of Vibrio cholerae toxin A (P01555), B (P01556), and P (P29485). A membrane-bound peptide library representing the three chains of the toxin was probed with a pool of vaccinated mouse sera (n = 15), and reactivity was detected using goat anti-mouse IgG alkaline phosphatase alkaline labeled secondary antibody and chemiluminescence substrate. Panel (A) presents an image of the peptide array showing reactivity as dark circles. The panels in (B) graphically present the percent signal after normalizing the signals to the positive and negative controls. The sequences of peptides in each position are listed in Supplementary Figure S1.
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Figure 2. Epitope localization in three-dimensional structures of V. cholerae toxins A (A), B (B), and P (C). Epitopes are presented in orange within models constructed using the crystal structure of toxin A (PDB: 1xtc) and the AlphaFoldDB predicted protein structures of toxins B and P. Images were created using PyMOL.
Figure 2. Epitope localization in three-dimensional structures of V. cholerae toxins A (A), B (B), and P (C). Epitopes are presented in orange within models constructed using the crystal structure of toxin A (PDB: 1xtc) and the AlphaFoldDB predicted protein structures of toxins B and P. Images were created using PyMOL.
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Figure 3. Structure of the three MAP4s synthesized for peptide ELISAs. The Wang resin used for F-moc peptide synthesis generated a dendrimer with four copies of the epitope (A) TxA-3, (B) TxB-11, and (C) TxP-15.
Figure 3. Structure of the three MAP4s synthesized for peptide ELISAs. The Wang resin used for F-moc peptide synthesis generated a dendrimer with four copies of the epitope (A) TxA-3, (B) TxB-11, and (C) TxP-15.
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Figure 4. Reactivity of vaccinated mice sera (n = 12) 15- and 30-days post vaccination against the MAP4 peptides Vc/TxA-3, Vc/TxB-11, and Vc/TxP-15 by ELISA. (A) Unvaccinated mice were used as a control (n = 30). The relative index was calculated using the cut-off of each MAP to normalize the measured value from the sera of 12 vaccinated mice. (B) The receiver operating characteristic curve (ROC) was used to determine the cut-off of peptide B (Vc/TxB-11; 0.053), reactivity (100%), specificity (100%), and AUC (0.9987). (****) Statistical significance at p < 0.0001.
Figure 4. Reactivity of vaccinated mice sera (n = 12) 15- and 30-days post vaccination against the MAP4 peptides Vc/TxA-3, Vc/TxB-11, and Vc/TxP-15 by ELISA. (A) Unvaccinated mice were used as a control (n = 30). The relative index was calculated using the cut-off of each MAP to normalize the measured value from the sera of 12 vaccinated mice. (B) The receiver operating characteristic curve (ROC) was used to determine the cut-off of peptide B (Vc/TxB-11; 0.053), reactivity (100%), specificity (100%), and AUC (0.9987). (****) Statistical significance at p < 0.0001.
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De-Simone, S.G.; Napoleão-Pêgo, P.; Gonçalves, P.S.; Lechuga, G.C.; Cardoso, S.V.; Provance, D.W., Jr.; Morel, C.M.; da Silva, F.R. B-Cell Epitope Mapping of the Vibrio cholera Toxins A, B, and P and an ELISA Assay. Int. J. Mol. Sci. 2023, 24, 531. https://doi.org/10.3390/ijms24010531

AMA Style

De-Simone SG, Napoleão-Pêgo P, Gonçalves PS, Lechuga GC, Cardoso SV, Provance DW Jr., Morel CM, da Silva FR. B-Cell Epitope Mapping of the Vibrio cholera Toxins A, B, and P and an ELISA Assay. International Journal of Molecular Sciences. 2023; 24(1):531. https://doi.org/10.3390/ijms24010531

Chicago/Turabian Style

De-Simone, Salvatore G., Paloma Napoleão-Pêgo, Priscilla S. Gonçalves, Guilherme C. Lechuga, Sergian V. Cardoso, David W. Provance, Jr., Carlos M. Morel, and Flavio R. da Silva. 2023. "B-Cell Epitope Mapping of the Vibrio cholera Toxins A, B, and P and an ELISA Assay" International Journal of Molecular Sciences 24, no. 1: 531. https://doi.org/10.3390/ijms24010531

APA Style

De-Simone, S. G., Napoleão-Pêgo, P., Gonçalves, P. S., Lechuga, G. C., Cardoso, S. V., Provance, D. W., Jr., Morel, C. M., & da Silva, F. R. (2023). B-Cell Epitope Mapping of the Vibrio cholera Toxins A, B, and P and an ELISA Assay. International Journal of Molecular Sciences, 24(1), 531. https://doi.org/10.3390/ijms24010531

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