Effect of Flavonoids on MCP-1 Expression in Human Coronary Artery Endothelial Cells and Impact on MCP-1-Dependent Migration of Human Monocytes
Abstract
:1. Introduction
2. Results
2.1. Flavonoids Do Not Impair the Viability of HCAEC at Low Concentrations
2.2. Flavonoids Do Not Impair the Cell Number of HCAEC at Low Concentrations
2.3. Quercetin, Kaempferol, and Luteolin Inhibit IL-1β-Induced MCP-1 mRNA Expression in a Concentration-Dependent Manner, Whereas Cannflavin A Increases MCP-1 mRNA Levels in HCAEC
2.4. Flavonoids Decrease IL-1β-Stimulated MCP-1 Secretion of HCAEC
2.5. Migration of Monocytes toward CM of IL-1β-Stimulated HCAEC Is Prevented by Treatment of HCAEC with Quercetin, Kaempferol, and Luteolin but Not with Cannflavin A
2.6. MCP-1 Is an Important Mediator of Monocyte Migration
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Cell Culture
4.3. Cellular Viability Assays
4.4. Quantitative Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR)
4.5. MCP-1 ELISA
4.6. Monocyte Migration Assay
4.7. Statistics
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Brüser, L.; Teichmann, E.; Hinz, B. Effect of Flavonoids on MCP-1 Expression in Human Coronary Artery Endothelial Cells and Impact on MCP-1-Dependent Migration of Human Monocytes. Int. J. Mol. Sci. 2023, 24, 16047. https://doi.org/10.3390/ijms242216047
Brüser L, Teichmann E, Hinz B. Effect of Flavonoids on MCP-1 Expression in Human Coronary Artery Endothelial Cells and Impact on MCP-1-Dependent Migration of Human Monocytes. International Journal of Molecular Sciences. 2023; 24(22):16047. https://doi.org/10.3390/ijms242216047
Chicago/Turabian StyleBrüser, Lea, Elisa Teichmann, and Burkhard Hinz. 2023. "Effect of Flavonoids on MCP-1 Expression in Human Coronary Artery Endothelial Cells and Impact on MCP-1-Dependent Migration of Human Monocytes" International Journal of Molecular Sciences 24, no. 22: 16047. https://doi.org/10.3390/ijms242216047
APA StyleBrüser, L., Teichmann, E., & Hinz, B. (2023). Effect of Flavonoids on MCP-1 Expression in Human Coronary Artery Endothelial Cells and Impact on MCP-1-Dependent Migration of Human Monocytes. International Journal of Molecular Sciences, 24(22), 16047. https://doi.org/10.3390/ijms242216047