Intelligent De Novo Design of Novel Antimicrobial Peptides against Antibiotic-Resistant Bacteria Strains
Abstract
:1. Introduction
2. Results and Discussion
2.1. Evaluating GAN-Designed Peptides In Silico
2.2. Evaluating GAN-Designed Peptides In Vitro
3. Materials and Methods
3.1. Collecting AMPs to Train the Model
3.2. The Architecture of the Proposed GAN
3.3. Mechanism of AMP Production
3.4. Training Process
3.5. Evaluation of GAN-Designed Sequences
3.6. GAN-Designed Sequence Selection for Experimental Validation
3.7. Strains and Reagents
3.8. Antimicrobial Assays
4. Conclusions and Future Work
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Bacteria Species | E. coli | S. aureus | P. aeruginosa | ||
---|---|---|---|---|---|
Strain | SG13009 | MSSA S01-10-0202 ※ | MRSA N07-10-0043 ※※ | S07-10-0059 * | M06-06-0213 ** |
polyphemusin I | 0.7 | >50 | >50 | >50 | >50 |
GAN-pep 1 | >50 | >50 | >50 | >50 | >50 |
GAN-pep 2 | 2 | >50 | >50 | 50 | 5 |
GAN-pep 3 | 2 | 6 | 45 | 3 | 3 |
GAN-pep 4 | 2 | >50 | >50 | 50 | 35 |
GAN-pep 5 | 22.5 | >50 | >50 | >50 | >50 |
GAN-pep 6 | >50 | >50 | >50 | >50 | >50 |
GAN-pep 7 | >50 | >50 | >50 | >50 | >50 |
GAN-pep 8 | 15 | 15 | 45 | >50 | >50 |
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Lin, T.-T.; Yang, L.-Y.; Lin, C.-Y.; Wang, C.-T.; Lai, C.-W.; Ko, C.-F.; Shih, Y.-H.; Chen, S.-H. Intelligent De Novo Design of Novel Antimicrobial Peptides against Antibiotic-Resistant Bacteria Strains. Int. J. Mol. Sci. 2023, 24, 6788. https://doi.org/10.3390/ijms24076788
Lin T-T, Yang L-Y, Lin C-Y, Wang C-T, Lai C-W, Ko C-F, Shih Y-H, Chen S-H. Intelligent De Novo Design of Novel Antimicrobial Peptides against Antibiotic-Resistant Bacteria Strains. International Journal of Molecular Sciences. 2023; 24(7):6788. https://doi.org/10.3390/ijms24076788
Chicago/Turabian StyleLin, Tzu-Tang, Li-Yen Yang, Chung-Yen Lin, Ching-Tien Wang, Chia-Wen Lai, Chi-Fong Ko, Yang-Hsin Shih, and Shu-Hwa Chen. 2023. "Intelligent De Novo Design of Novel Antimicrobial Peptides against Antibiotic-Resistant Bacteria Strains" International Journal of Molecular Sciences 24, no. 7: 6788. https://doi.org/10.3390/ijms24076788
APA StyleLin, T. -T., Yang, L. -Y., Lin, C. -Y., Wang, C. -T., Lai, C. -W., Ko, C. -F., Shih, Y. -H., & Chen, S. -H. (2023). Intelligent De Novo Design of Novel Antimicrobial Peptides against Antibiotic-Resistant Bacteria Strains. International Journal of Molecular Sciences, 24(7), 6788. https://doi.org/10.3390/ijms24076788