The Development of a Portable SPR Bioanalyzer for Sensitive Detection of Escherichia coli O157:H7
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Methods
2.3. Immobilization of the Escherichia coli O157:H7 Antibody on the Au Film Surface
3. Results and Discussion
3.1. Evaluation of the Response Units from the SPR Biosensor for the Biomolecular Recognition Membrane
3.2. Samples Analysis of E. coli O157:H7
3.3. Comparison with the E. coli O157:H7ELISA Kit
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Diluted Factors | Original Positive Sample | 10 | 20 | 40 | 80 | 160 |
---|---|---|---|---|---|---|
SPR bioanalyzer | + | + | + | + | + | + |
ELISA kit | + | + | + | + | − | − |
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Wang, S.; Xie, J.; Jiang, M.; Chang, K.; Chen, R.; Ma, L.; Zhu, J.; Guo, Q.; Sun, H.; Hu, J. The Development of a Portable SPR Bioanalyzer for Sensitive Detection of Escherichia coli O157:H7. Sensors 2016, 16, 1856. https://doi.org/10.3390/s16111856
Wang S, Xie J, Jiang M, Chang K, Chen R, Ma L, Zhu J, Guo Q, Sun H, Hu J. The Development of a Portable SPR Bioanalyzer for Sensitive Detection of Escherichia coli O157:H7. Sensors. 2016; 16(11):1856. https://doi.org/10.3390/s16111856
Chicago/Turabian StyleWang, Shun, Jiufeng Xie, Min Jiang, Keke Chang, Ruipeng Chen, Liuzheng Ma, Juanhua Zhu, Qingqian Guo, Haifeng Sun, and Jiandong Hu. 2016. "The Development of a Portable SPR Bioanalyzer for Sensitive Detection of Escherichia coli O157:H7" Sensors 16, no. 11: 1856. https://doi.org/10.3390/s16111856
APA StyleWang, S., Xie, J., Jiang, M., Chang, K., Chen, R., Ma, L., Zhu, J., Guo, Q., Sun, H., & Hu, J. (2016). The Development of a Portable SPR Bioanalyzer for Sensitive Detection of Escherichia coli O157:H7. Sensors, 16(11), 1856. https://doi.org/10.3390/s16111856