Long-Read Sequencing Unlocks New Insights into the Amphidinium carterae Microbiome
Abstract
:1. Introduction
2. Results
2.1. Bacterial Growth
2.2. Bacterial Identification
NCBI Sample Title 1 | Length | AT% |
---|---|---|
Labrenzia sp. strain ac12 | 6,053,707 | 40.9 |
Hoeflea alexandrii strain ac4 | 4,829,646 | 38.4 |
Algihabitans albus strain ac2 | 4,739,774 | 34.3 |
Ahrensia marina strain ac1 | 4,424,055 | 42.8 |
Seohaeicola saemankumensis strain ac14 | 4,275,009 | 36.6 |
Roseovarius mucosus strain ac13 | 3,969,289 | 38.9 |
Rhodophyticola porphyridii strain ac11 | 3,902,216 | 35.8 |
Oceaniradius stylonematis strain ac10 | 3,664,187 | 35.3 |
Oceanicaulis alexandrii strain ac9 | 2,992,841 | 37.7 |
Nitratireductor sp. strain ac15 | 2,917,504 | 39.8 |
Marinobacter adhaerens strain ac5 | 4,424,055 | 42.9 |
Cyclobacterium xiamenense strain ac3 | 5,806,256 | 51.6 |
Marivirga tractuosa strain ac6 | 4,787,102 | 65.3 |
Muricauda sp. strain ac8 | 4,366,883 | 58.0 |
Phycisphaeraceae SM1A02 strain ac7 | 3,415,114 | 34.9 |
2.3. Bacterial Abundance
2.4. Bacterial Metabolic Pathways
2.5. Secondary Metabolite Synthesis
2.6. Antibiotic Resistance
3. Discussion
4. Materials and Methods
4.1. Cell Culturing
4.2. DNA Extraction and Long-Read Sequencing
4.3. Genome Assembly
4.4. Phylogenetic Analysis
4.5. Read Abundance Mapping
4.6. Metabolic and Secondary Metabolites Analysis
4.7. Antimicrobial Resistance Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Judd, M.; Wira, J.; Place, A.R.; Bachvaroff, T. Long-Read Sequencing Unlocks New Insights into the Amphidinium carterae Microbiome. Mar. Drugs 2024, 22, 342. https://doi.org/10.3390/md22080342
Judd M, Wira J, Place AR, Bachvaroff T. Long-Read Sequencing Unlocks New Insights into the Amphidinium carterae Microbiome. Marine Drugs. 2024; 22(8):342. https://doi.org/10.3390/md22080342
Chicago/Turabian StyleJudd, Miranda, Jens Wira, Allen R. Place, and Tsvetan Bachvaroff. 2024. "Long-Read Sequencing Unlocks New Insights into the Amphidinium carterae Microbiome" Marine Drugs 22, no. 8: 342. https://doi.org/10.3390/md22080342
APA StyleJudd, M., Wira, J., Place, A. R., & Bachvaroff, T. (2024). Long-Read Sequencing Unlocks New Insights into the Amphidinium carterae Microbiome. Marine Drugs, 22(8), 342. https://doi.org/10.3390/md22080342