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Article

Is the Survivability of Silver Fir under Condition of Strong Ungulate Pressure Related to Mycobiota of Bark-Stripping Wounds?

by
Wojciech Pusz
1,
Anna Baturo-Cieśniewska
2,
Agata Kaczmarek-Pieńczewska
1,*,
Katarzyna Patejuk
1 and
Paweł Czarnota
3,4
1
Department of Plant Protection, Division of Plant Pathology and Mycology, Wrocław University of Environmental and Life Sciences, Plac Grunwaldzki 24a, 50-363 Wroclaw, Poland
2
Laboratory of Molecular Mycology, Phytopathology and Entomology, Department of Biology and Plant Protection, UTP University of Science and Technology, Aleja Kaliskiego 7, 85-796 Bydgoszcz, Poland
3
Department of Ecology and Environmental Protection, University of Rzeszów, Zelwerowicza 4 Street, 35-601 Rzeszów, Poland
4
Scientific Laboratory of the Gorce National Park, Poręba Wielka 590, 34-735 Niedźwiedź, Poland
*
Author to whom correspondence should be addressed.
Forests 2021, 12(8), 976; https://doi.org/10.3390/f12080976
Submission received: 10 June 2021 / Revised: 16 July 2021 / Accepted: 19 July 2021 / Published: 23 July 2021
(This article belongs to the Special Issue Forest Health Management: Interaction between Forest Trees, Pathogens and Environment)
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Abstract

:
The aim of the research was to check whether the healing of bark-stripping wounds of the silver fir tree trunks reduces the share of wood-decomposing fungi, which may be the result of inter-species interactions. The study carried out in Gorce National Park in Polish Western Carpathians analyzed drill holes of sapwood from three types of wounds (fresh, healed and old) on fir trunks with a diameter at breast height (DBH) of 4.0–16.9 cm as a result of bark-stripping by red deer (Cervus elaphus). In the wood of fresh wounds Alternaria alternata (Fr.) Keissl. and Arthrinium arundinis (Corda) Dyko & B. Sutton had the largest share in mycobiota. Phompsis spp. and the species Sydowia polyspora (Bref. & Tavel) E. Müll. and Epicoccum nigrum Link were also isolated. The dominants in old wounds were Eutypa spp., Phomopsis spp. and Cylindrobasidium evolvens (Fr.) Jülich. Healed wounds were dominated by Trichoderma atroviride P. Karst, a fungus antagonistic to many fungal pathogens. Such properties are shared by A. arundinis, especially common in fresh wound wood. It seems that these fungi support the process of wounded tree regeneration (healing of wounds) and limit the activity of wood-decaying fungi in old age, which makes fir survival very high. Thus, even a strong red deer pressure cannot be considered the basic factor determining the dynamics of fir in this part of the Carpathians.

1. Introduction

The bark stripping by ungulates is the result of their need for food and specific nutrients contained in the bark, including different fiber fractions [1] in the hard-to-survive winter period [2], but also in summer [3]. This method of a diet supplementing is typical for forest representatives of Cervidae including genera Alces, Capreolus, Cervus, Dama, Odocoileus, Rangifer [1] and, unless it causes direct, serious economic damage to forests, is generally acceptable and these animals are treated as key factors of natural ecological processes shaping inter-species relationships and the diversity and structure of forest plant communities [4,5]. Open wounds, however, allow the infection of wood-inhabiting fungi, the presence of which is already most often perceived negatively in commercial forests.
The problem of young tree bark-stripping and then wound colonization, which in turn may lead to infection of tree tissues by pathogenic fungi, has long been noticed by researchers. The first Polish reports about the significant impact of bark-stripping by red deer (Cervus elaphus) on the infection of trees by fungi come from the Karkonosze Mountains in the 1960s. Domański [6] found that open wounds on spruce trunks facilitate infection by saprophytes and pathogens. Among the potential perpetrators of wood decay, he mentions fungi of the genus Stereum, but also species currently included in the so-called Deuteromycota fungi. His results were later confirmed by many researchers, who isolated many dangerous pathogens from bark-stripped spruce trunks, such as Heterobasidion spp. [7]. The study also confirmed the presence of the species of fungi that were then classified as Deuteromycota, and which are responsible for the so-called gray or mildew rot of wood [8]; their role may be much more important in the process of wood decomposition than previously thought [7,8].
Arhipova et al. [9] studied, among others, the species composition of fungi occurring in Pinus contorta Douglas ex Loudon stem wounds, resulting from elk and deer bark-stripping. Researchers isolated a total of 28 taxa of fungi found in more than half of the wood samples tested. Ascomycota anamorphs was the dominating species. However, studies by many authors reveal different sensitivity of individual tree species to bark-stripping and, consequently, to fungal infections and the appearance of wood rot. In the case of fir, the species composition of fungi inhabiting wounds has not been studied so far [10].
Silver fir (Abies alba Mill.) is the sixth most productive forest-forming species in Europe [11]. Its native distribution ranges from the Pyrenees in the west to the Carpathians in the east and from the Polish lowlands in the north to southern Italy in the south [12]. In the Carpathians, it is one of the three main forest-forming species, next to beech and spruce. It is most numerous in the Abieti-Piceetum montanum fir-spruce forests and in the fir variant of the Carpathian beech forest Dentario glandulosae-Fagetum abietetosum [13]. The situation is similar in Gorce, in the Polish Western Carpathians [14,15], where Gorce National Park was established in 1981 on an area of just over 7000 ha. Its strategic goal is to preserve or restore natural features to forests through strict protection of spontaneous natural processes in the ever-larger territory of the park, currently implemented on an area of 4009 ha. One such process is red deer pressure on forests, which results in damaging the young generation of silver fir by browsing and bark-stripping [16,17]. This phenomenon is generally perceived negatively by many people, especially by foresters, hunters and locals sharing space with wild herbivorous [18,19]. The pressure of these animals on forests is also controversial for nature protection services themselves, which often identify their mission with the need to protect the forest using management methods and to treat fir as an endangered species that requires special care [20]. This view is largely the result of the phenomenon of mass extinction of fir in Europe in the second half of the last century [21,22], and the unique food preference of this forest-forming species by deer [20,23,24]. It is also supported by the usually locally poor regeneration capacity of fir stands, which, due to climatic, geomorphological and lighting conditions, can be highly variable and irregular in time and space [25,26,27], depending on the occurrence of the ‘rare windows of opportunity’ for fir [23].
The conviction about the poor condition of the fir, despite the noble cause of wildlife preservation, is the reason for the so-called active protection measures in Polish Carpathian national parks, consisting in securing treetops with plastic spirals, and trunks of young firs with repellents [20]. Even if data from various regions of the Carpathians indicate that fir is currently in expansion [25,28]; that the condition of fir trees which underwent bark-stripping, expressed in terms of growth rate and tree vitality, does not significantly differ from the “normal” ones [10]; and that the survival rate of the young generation of this species, despite intensive gnawing, is very high [20,29], there are still worries about its health. The perspective of future forests, including natural ones, in which bark-stripped fir trees will not grow old due to the destructive fungi developing in the wood, raises concerns. These fears, as well as the desire to learn about the diversity of fungi inhabiting the wood of fallen firs in different thickness classes, their phytopathological significance and, finally, possible inter-species interactions in the communities of these fungi, became the reason for conducting this study.
The working hypotheses that we wanted to verify are: (1) the type of bark stripping wound and DBH of trunk determine wood inhabiting fungal diversity and the share of wood-decomposing fungi, and (2) the natural reduction in the share of wood-decomposing fungi in healing silver fir trunks is due to fungal species interactions inhabiting wounds, which results in high regenerative capacities of this forest-forming species. The last hypothesis was based on the results of the study by Pach [10], who found that with age, there is a significant decrease in the rate of decay and spread of wood discoloration of bark-stripped fir trees.
The obtained results may provide the answer to the following pragmatic question: whether in forest habitats dominated by fir (or in the reconstruction phase), particularly in national parks, it is necessary to apply cost-intensive, mechanical (plastic spirals for apical shoots) and chemical (repellents) protection of this forest-forming species against red deer. It is all the more important as, in the time of climate change and the progressive decay of mountain spruce stands, fir seems to be a species of the future [13,25].

2. Materials and Methods

2.1. Fieldwork

The research was carried out in the Gorce National Park (Western Carpathians, Southern Poland) within the active protection zone covered with young (early optimal growth stage) fir-spruce forest of the lower belt Abieti-Piceetum montanum (Figure 1) extending on acid brown soil. Wood samples were taken with a Pressler drill from a sapwood part of silver fir trunks, referring to three categories of wounds: fresh wounds marked with the symbol “F” (Figure 2A), healed wounds marked with the symbol “H” (Figure 2B) and old, and open wounds marked with the symbol “O” (Figure 2C). The drill was decontaminated with Aerodesin 2000 before each drilling.
The study covered trees of various age classes, corresponding to the following values of a diameter at breast height (DBH): 4.0–6.9 cm, 7.0–9.9 cm, 10.0–12.9 cm, 13.0–16.9 cm. The sole criterion for selecting the sample trees was that they had achieved the established thickness class DBH and wound type, so that each wound type in each thickness class was represented by ± 10–12 trees. This choice was firstly determined by the shortest distance between the fir tree and the linear temporal transect in each of the four heavily bark stripped regions, and in the absence of trees meeting the criteria, they were selected randomly from the immediate vicinity. A total of 141 wood samples from bark-stripping wounds were collected (Table 1). Additionally, wood samples were collected in the same way from 11 firs with DBH between 30.0–40.0 cm with no visual signs of bark-stripping, growing in the vicinity of damaged trees. These trees were taken as a reference sample, assuming that in the past they were also subjected to similar pressure of red deer. It was assumed that the healing of wounds and further growth of these trees is the result of high regenerative abilities of this tree species, resulting from the natural mechanism allowing them to neutralize pathogenic fungi inhabiting wounds sustained at younger age.

2.2. Laboratory Analysis

The pieces of wood were placed on MEA medium (Malt Extract Agar, Difco, La Vista, NE, USA) in accordance with the methodology proposed by Arhipova et al. [9] and PDA medium (Potato Dextrose Agar, Difco, Lawrence, KS, USA). Growing colonies of fungi were passaged and then clean mycelium was obtained by the method of monospore cultures, meeting the requirements of the molecular method of determining species affiliation.
Isolates grown in Petri dishes with PDA, showing morphological diversity, and representing various taxonomic units with high probability, were used for molecular analyses based on the sequence of ITS rDNA regions. The subject of molecular identification were representatives separated on the basis of microscopic morphological features suggesting their belonging to different species/genera. The aim of the analysis of ITS regions, in addition to the identification of the analyzed isolates, was to graphically depict the genetic connections between isolates and enrich the NCBI international database with their sequences. From pure cultures of individual isolates grown on PDA, three mycelial discs 5 mm in diameter were cut and transferred to Petri dishes with PDB glucose-potato liquid medium (Potato Dextrose Broth, A&A Biotechnology, Gdańsk, Poland), and protected with parafilm. The mycelium obtained after 5–7, and in the case of the slowest growing specimens—after 10 days, was rinsed with sterile water, filtered using a filtering set consisting of filter paper, a Büchner funnel and a vacuum pump, and then lyophilized for 2 days in a Cool Safe freeze dryer (Scanvac, Lynge, Denmark). DNA isolation was performed according to the modified method of Doyle and Doyle [30]. Thirty milligrams of mycelium of each isolate homogenized in a Magna Lyser homogenizer (Roche, Basel, Switzerland) using quartz beads and quartz sand was placed in a 2 mL tube and covered with 900 µL of extraction buffer containing CTAB 5.0%, EDTA 0.5 M, NaCl 5.0 M, Tris -HCl (pH 8.0) 1.0 M, 2-mercaptoethanol and PVP 2.0%. After incubation at 65 °C, phenol, chloroform and isoamyl alcohol were used to remove proteins and carbohydrates. In the following stages, 95% and 70% ethyl alcohol were used. The obtained DNA was suspended in 200 µL of ddH2O and cleaned with an anti-inhibitor kit (A&A Biotechnology, Gdańsk, Poland).
DNA was measured fluorometrically on a Quantus device (Promega, WI, USA) and diluted in ddH2O to a concentration of 10 ng·μL−1 for further analysis. The PCR reaction aimed to amplify the ITS regions was performed in a volume of 37.5 μL containing the PCR Core Kit reagents (QIAGEN, Germantown, MD, USA): 1x buffer, 1x Q solution, 1 mM MgCl2, 0.2 mMdNTP, 0.6 pM of each of the two primers (ITS1: 5′-TCCGTAGGTGAACCTGCGG-3′ and ITS4: 5′-TCCTCCGCTTATTGATATGC-3′, White et al. [31] and DNA with a concentration of 10 10 ng·μL−1. DNA was amplified in an Eppendorf EP Mastercycler according to the reaction protocol: pre-denaturation 3 min at 95 °C—3 min, 35 cycles (95 °C—1 min, 55 °C—45 s, 72 °C—1 min) and final elongation at 72 °C—10 min. The presence of reaction products was verified after electrophoretic separation in TBE buffer, carried out on a 1.2% agarose gel (Pronadisa, Spain) with the addition of Simply Safe (EURx, Poland) dye, applying 2 μL of the post-reaction mixture.
The amplification products were purified and sequenced by Genomed (Poland). FinchTV 1.4 (Geospiza Inc., WA, USA) was used to analyze the sequences obtained. ClustalW analysis was performed on Mega7 Toolbar [32] (accessed on 10 October 2020). The Basic Local Alignment Search Tool (BLAST) in the NCBI database (The National Center for Biotechnology Information) [33] (accessed on 10 October 2020) was used to identify species based on the ITS sequence. The Mega7 Toolbar tools [32] were used to determine the sequence differentiation (overall mean distance) and dendrogram construction based on the ITS rDNA sequence using the maximum likelihood algorithm (MLE) based on the Kimura 2-parameter model [34,35].
The nomenclature of the identified taxa is in accordance with the Catalogue of Life [36] (accessed on 10 October 2020) and the Index Fungorum [37] (accessed on 10 October 2020).

2.3. Statistical Analysis

Based on the achieved results the differences in the abundance of wood decaying fungi inhabiting three types of bark-stripping wounds (plus control) have been shown on a box-plot graph. The same type of graph was used to show differences in the fungal diversity of these wounds. For this purpose, the three α-diversity indexes were calculated: Shannon-Wiener (H), Pielou (J) and Simpson (D), based on mathematical equation presented in Patejuk and Pusz [38]. β-biodiversity Sørensen index was calculated separately. Moreover, the linear regression between fungal colony abundance on types of bark wounds and the number of annual rings and DBH was calculated. Graphical representations and basic statistics were made in Tableau (2020.2.4 Professional Edition).

3. Results

3.1. Taxonomy and Frequency of Fungal Colonies

A total of 41 taxa of fungi were isolated and assigned to a species or higher systematic rank (Table 2, Figure 3). Trichoderma atroviride P. Karst and the fungi of the genus Eutypa were dominant among all the isolated fungal colonies, reaching the frequency of nearly 24% and 13%, respectively. There were also relatively many colonies of such taxa as: Phomopsis spp. (10.19%), Cylindrobasidium evolvens (Fr.) Jülich (8.57%), Paraphaeosphaeria neglecta Verkley, Riccioni & Stielow (8.29%), Arthrinium arundinis (Corda) Dyko & B. Sutton (7.71%), and Alternaria alternata (Fr.) Keissl. (6.67%). The level of remaining taxa colonizing bark-stripping wounds ranged from 3.4 to 0.1% (Figure 4).

3.2. Fungi-Wounds Relationship

When analyzing the obtained results, it can be concluded that the most fungal colonies were isolated from old wounds (434 colonies and 17 taxa). For fresh and scarred wounds, this number was 249 (18 taxa) and 212 (24 taxa), respectively, compared to the total number of 154 (nine taxa) colonies obtained from the control drills (Table 3). In the case of fresh wounds, species such as A. alternata and A. arundinis had the highest share. Fungi belonging to the genus Phomopsis were also isolated, as well as the species Sydowia polyspora (Bref. & Tavel) E. Müll. and Epicoccum nigrum Link. In the case of fresh wounds, T. atroviride was a distinct dominant, which also dominated in the mycobiota of wood collected from control trees. On the other hand, in the case of old wounds, the fungi of the genera Eutypa and Phomopsis, as well as the fungus C. evolvens, were dominant.
The number of colonies isolated from the samples was highly variable depending on the sample, but the median for the examined wounds was 0 for fresh wounds, 1 for healed wounds and 7 for old wounds (Figure 5). Control samples had the highest number of colonies, and in this case the distribution of the number of colonies isolated from individual samples had the features of normal distribution.
When analyzing wounds on fir trunks in terms of their DBH, it was found that the largest number of colonies occurs in thinner trees (DBH 4.0–6.9 cm). In their case, 306 colonies belonging to 25 taxa were obtained. In trees thicker than 7.0 cm, the number of colonies ranged from 113 to 271, with 32 taxa. The fungi of the genus Eutypa and Phomopsis had the highest share (Table 4).
The impact of the measured parameters of trees, i.e., the number of annual rings and DBH, on the number of fungal colonies isolated from sapwood drillholes from individual types of wounds is interesting (Figure 6). The number of isolates from old wounds was not significantly influenced by either the number of annual rings (age of the trees) or the DBH of firs. A very weak positive correlation at the level of R2 = 0.01229 was found between the number of colonies inhabiting scarred wounds and the age of the trees, while the DBH of the sampled firs was not significant. Significant differences were found in the case of fresh wounds, where the number of isolated colonies decreased with the increasing number of annual rings and DBH. The detected correlation was average and depending on the parameter was R2 = 0.2015 for annual rings and R2 = 0.1205 for DBH.

3.3. Wounds-Fungal Diversity Relationship

On the basis of the obtained results for samples with all variables taken into account (location, type of wound and part of wood), α- and β-biodiversity indexes were calculated, describing the differences between the studied populations. Detailed results are presented in Table 5 and Table 6. If no isolates were identified from a given sample, they were not included in the biodiversity analysis (Table 5). Samples of very poor biodiversity (1–2 taxa in the sample), which do not meet the requirements for calculations, are marked with “0” values (Table 5).
The most common values of the Shannon-Wiener and Simpson diversity indices for all trials with healed wounds were lower than the values of these indices for fresh and old wounds (Figure 7). The range of the Pielou evenness index values was much wider for the mycobiota of wounds compared to the control and for the other types of wounds, indicating that these wounds are inhabited by fungal communities, often dominated by a few species, among which T. atroviride plays a major role (Figure 2).
On the basis of the Sørensen’s β-biodiversity index, it was estimated that the most unique mycobiota were found in healed wounds that were least similar to controls (0.34) or old wounds (0.38) (Table 6). Fresh and old wounds showed the highest group similarity index (0.63).

4. Discussion

In the case of fresh wounds, species such as A. alternata and A. arundinis had the highest share. Fungi belonging to the genus Phomopsis as well as S. polyspora and E. nigrum were also isolated. These fungi, considered to be secondary pathogens, can also act as saprotrophs. They were also isolated by other researchers in wood at the initial stages of decomposition [39,40]. Particularly noteworthy is the large share of A. arundinis fungus in fresh wounds, the anti-fungal activity of which has been documented [41]. This fungus was also isolated in research on the mycobiota of fallen spruces [6]. Scar wounds were most frequently inhabited by T. atroviride, which was also dominant in the mycobiota of wood collected from control trees. As shown by recent molecular studies, this species may be a widespread endophyte [42,43], showing anti-fungal properties, which have been recently used to protect trees against wood-decaying fungi [44,45,46,47]. In Gorce, T. atroviride also numerously inhabited the wood of bark-stripped firs with a DBH above 13.0 cm. Perhaps the remarkable natural regenerative abilities of silver fir is the consequence of T. atroviride domination in the mycobiota of healed wounds of thicker trees and in the wood of visually undamaged control trees with DBH > 30.0 cm. As shown by the research on the dynamics of stands carried out in the Gorce National Park on permanent monitoring plots [14], despite the persistent strong pressure of red deer for years, expressed in the share of bark-stripped firs with DBH < 17.0 cm at the level of 80% of their numbers [15,48], in the last 25 years, the density of this forest-forming species in the tree layer has increased almost threefold, from 78 trees/ha in 1992 to 216 trees/ha in 2017, and the percentage share of fir in the stand increased from 16% to 36% [48]. The survival rate of this species, estimated on the basis of the number of trees reaching DBH 7.0–16.9 cm in 1992 that survived until 2017, ranges from 93% to 100%, depending on the thickness class (Table 7).
In turn, Pach [29], while examining the influence of red deer on the cultivation values of fir stands in the Beskid Sądecki, another range of the Polish Carpathians, came to the assumption that the occurrence of damage as a result of bark-stripping in fir regeneration in the undergrowth stage, is probably not dangerous for trees, as compared to the damage in later development stages. In light of the above, the results of the studies by Niemtur et al. [13] who, while assessing the health of old fir trunks by using acoustic tomography method, found that in a random sample (mean age 99 years; N = 30) in the Gorce National Park, almost 80% of trees did not have butt rot. The share of healthy wood in this part of measured trunks reached 94%, despite the fact that the dimensions of the Gorce fir trees in the entire collection of 450 trees in the entire fir range in Poland were the highest.
The fungi of the genus Eutypa had a relatively large share in the mycobiota of fir trees that underwent bark-stripping. They dominated in the case of thinner trees and were isolated from old wounds. They are dangerous pathogens for many crops that can also infect wood and occur together with other species of wood-decaying fungi [49,50,51]. The conducted research recorded the presence—along with Eutypa spp.—of the fungus C. evolvens in old wounds, which may participate in wood decomposition [52,53]. A similar situation occurs in the case of the Phomopsis genus, which very often colonize the wounds of fir [54,55] and are known carcinogenic fungi [56].
The actual role of inter-species competition of fungi in fir bark-stripped by red deer and the assessment of the importance of fungi showing inhibitory properties in the natural defense mechanism of this forest-forming species against destructive fungi will be the subject of a separate study.

5. Conclusions

  • The greatest number of fungal colonies inhabiting the silver fir wood was obtained from old open wounds, with a smaller number of taxa compared to fresh and healed wounds.
  • The age and DBH of bark-stripped trees do not seem to be of importance for the degree of infection of wounds expressed by the number of colonies; nevertheless, species diversity of wood decay fungi assessed by the α-diversity Shannon-Wiener (H) and Simpson (D) indices seems to be lower in healed wounds.
  • The strong presence of Arthrinium arundinis and Trichoderma atroviride in firs wounds, which are often antagonistic to pathogenic fungi, may indicate the natural defense mechanisms of fir, aimed at inhibiting disease processes and, subsequently, the decomposition of wood.
  • The high survival rate of the young generation of fir indicates that despite the strong pressure of red deer, the fir regeneration mechanism based on inter-species competition of fungi may be an effective tool in the fight for the survival of this species in the Carpathians.

Author Contributions

Conceptualization—W.P. and P.C.; methodology—W.P., A.B.-C., A.K.-P. and P.C.; investigation—W.P., A.B.-C., A.K.-P. and P.C.; formal analysis—K.P.; writing—original draft preparation—W.P., A.B.-C., A.K.-P., K.P. and P.C.; writing—review and editing—W.P., A.K.-P. and P.C.; supervision—W.P. All authors have read and agreed to the published version of the manuscript.

Funding

The research was funded by the Forest Fund under the agreement concluded between the State Forests National Forest Holding and the Gorce National Park in 2020 (agreement no. EZ.0290.1.6.2020, entitled “Activities of nature protection using forest management methods and other activities carried out in the Gorce National Park in 2020”).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Data sharing not applicable.

Acknowledgments

The authors would like to thank the Forest Fund for funding and financial support.

Conflicts of Interest

The authors declare that they have no conflict of interest.

Ethics

The samples in the study were collected on the area that belonged to the Gorce National Park and the authors received full permission to conduct the study. The experimental materials did not involve any humans or animals.

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Forests 12 00976 g001 550
Figure 1. Localities of sampled forest area within fir-spruce mountain forest Abieti-Piceetum montanum in the Gorce National Park (W Carpathians, S Poland). Abbreviations: J—Suhora forest district, Jasionów forest area, K—Kudłoń forest district, Za Palacem forest area, P—Kudłoń forest district, Pasieka forest area, S—Suhora forest district, Czarny Groń forest area.
Figure 1. Localities of sampled forest area within fir-spruce mountain forest Abieti-Piceetum montanum in the Gorce National Park (W Carpathians, S Poland). Abbreviations: J—Suhora forest district, Jasionów forest area, K—Kudłoń forest district, Za Palacem forest area, P—Kudłoń forest district, Pasieka forest area, S—Suhora forest district, Czarny Groń forest area.
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Forests 12 00976 g002 550
Figure 2. (A–C) Types of studied fir wounds caused by red deer (Cervus elaphus): (A)—fresh wound “F”, (B)—healed wound “H”, (C)—old wound “O”.
Figure 2. (A–C) Types of studied fir wounds caused by red deer (Cervus elaphus): (A)—fresh wound “F”, (B)—healed wound “H”, (C)—old wound “O”.
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Forests 12 00976 g003 550
Figure 3. A dendrogram based on the sequencing of ITS regions in MEGA7 software, depicting the level of genetic similarity between 66 isolates obtained from silver fir wood in the Gorce National Park in 2020.
Figure 3. A dendrogram based on the sequencing of ITS regions in MEGA7 software, depicting the level of genetic similarity between 66 isolates obtained from silver fir wood in the Gorce National Park in 2020.
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Forests 12 00976 g004 550
Figure 4. Percentage share of fungal taxa inhabiting wood of three types of wounds caused by red dear on silver fir trunks and wood of control fir trees (species constituting less than 5% in total of fungal colonies were grouped to “Other”).
Figure 4. Percentage share of fungal taxa inhabiting wood of three types of wounds caused by red dear on silver fir trunks and wood of control fir trees (species constituting less than 5% in total of fungal colonies were grouped to “Other”).
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Forests 12 00976 g005 550
Figure 5. Box-plot representation of the abundance of fungal colonies in the wood sample, according to the type of wound on silver fir trunk.
Figure 5. Box-plot representation of the abundance of fungal colonies in the wood sample, according to the type of wound on silver fir trunk.
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Forests 12 00976 g006 550
Figure 6. Linear regression of fungal colony abundance on three types of bark wounds against the number of annual rings and DBH of investigated silver fir trunks.
Figure 6. Linear regression of fungal colony abundance on three types of bark wounds against the number of annual rings and DBH of investigated silver fir trunks.
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Forests 12 00976 g007 550
Figure 7. Box-plot representation of the biodiversity in three types of wounds of fir trunk, based on α-diversity indexes: Shannon-Wiener diversity index (H), Pielou evenness index (J) and Simpson diversity index (D).
Figure 7. Box-plot representation of the biodiversity in three types of wounds of fir trunk, based on α-diversity indexes: Shannon-Wiener diversity index (H), Pielou evenness index (J) and Simpson diversity index (D).
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Table
Table 1. The number of collected drill samples of sapwood in reference to the category of bark-stripping wounds of Abies alba stems.
Table 1. The number of collected drill samples of sapwood in reference to the category of bark-stripping wounds of Abies alba stems.
DBH [cm]Fresh WoundsHealed WoundsOld Wounds
4.0–6.9131312
7.0–9.9111212
10.0–12.9121512
13.0–16.951014
Sum415050
Control > 30.011
In total152
Table
Table 2. Fungi isolated from sapwood of bark-stripped Abies alba. Molecular identification based on ITS rDNA region sequences using BLAST method and maximum likelihood ITS phylogeny.
Table 2. Fungi isolated from sapwood of bark-stripped Abies alba. Molecular identification based on ITS rDNA region sequences using BLAST method and maximum likelihood ITS phylogeny.
Fungal TaxaNumberFrequency [%]Accession Number in NCBIIdentity with NCBI Isolates (Accession Number)
Alternaria alternata (Fr.) Keissl.706.67MW090865MT644140, MT487778
Arthrinium arundinis (Corda) Dyko & B. Sutton817.71MW090861 MW090870 MW113167 MW090903 MW113168 MW113226
MW113227
MW113228		MT582801, MT446201
Aureobasidium pullulans (de Bary & Löwenthal) G. Arnaud40.38MW090923MT363099, MN922125
Botrytis cinerea Pers.80.76MW090881MT573470, MN448502
Cadophora sp.232.19MW090880 MW090922MF782737, MF188972
Chaetomium sp.20.19MW090810MH171491, KC963908
Coniochaeta sp.10.10MW090815MH859071, MG905629
Coprinellus micaceus (Bull.) Vilgalys, Hopple & Jacq. Johnson30.29MW090910MH179313, GU227721
Cucurbitariaceae sp.10.10MW090913KC963916, MK460387
Cylindrobasidium evolvens (Fr.) Jülich908.57MW113169MN947592, MH854673
Cystobasidium larynges (Reiersöl) Yurkov, Kachalkin, H.M. Daniel, M. Groenew., Libkind, V. de García, Zalar, Gouliam., Boekhout & Begerow10.10MW090823MH047192, KY103134
Cytospora sp.191.81MW090820 MW090909KY051899, KU516449
Epicoccum nigrum Link343.24MW090813MT548679, LC543647
Eutypa sp.12912.29MW090805 MW090860 MW090912 MW090911AY620998, KF453561
Fusarium acuminatum Ellis & Everh.60.57MW090924MT649858, MT635295
Fusarium avenaceum (Fr.) Sacc.10.10MW090925MT446118, MT276139
Fusarium tricinctum (Corda) Sacc.151.43MW090875 MW090907 MW090918MK934343, KC311496
Helotiales sp.10.10MW090817DQ317330, MF494618
Heterobasidion annosum (Fr.) Bref.10.10MW090819MK395162, KU727784
Neonectria neomacrospora (C. Booth & Samuels) Mantiri & Samuels50.48MW090919MH580206, MG049669
Nigrospora oryzae (Berk. & Broome) Petch90.86MW090868MT556421, MG661721
Paraphaeosphaeria neglecta Verkley, Riccioni & Stielow878.29MW090864 MW090874 MW090905 MW090906MK646057, MN244542
Penicillium chrysogenum Thom171.62MW090830MT524448, MK762610
Peniophoraceae sp.60.57MW090901MH010048, MH857634
Phomopsis sp.10710.19MW090829 MW090876MN538335, MT877030
Preussia minima (Auersw.) Arx30.29MW090811MG457827, MT645911
Rhizosphaera macrospora Gourb. & M. Morelet40.38MW090806AM884745, MN538337
Rhizosphaera oudemansii Maubl.10.10MW090827KU516578, EU700366
Sarea difformis (Fr.) Fr.50.48MW090809MN699648, FR837921
Schizophyllum commune Fr.90.86MW113225MT647523, MT601951
Sordaria fimicola (Roberge ex Desm.) Ces. & De Not.10.10MW090812MK965099, KX986578
Stereum sanguinolentum (Alb. & Schwein.) Fr.60.57MW090831AY618670, AF533962
Sydowia polyspora (Bref. & Tavel) E. Müll.282.67MW090808 MW090828 MW090873KY659505, KU837235
Tolypocladium sp.20.19MW090807MN096582, MH730171
Trametes versicolor (L.) Lloyd20.19MW090818 MW090825KJ995921, EU661891
Trametes sp.30.29MW090814MK343672, MK269115
Trichoderma atroviride P. Karst24823.62MW090869MT341775, MT023026
Truncatella angustata (Pers.) S. Hughes50.48MW090908MT514378, MK647988
Valsa sp.10.10MW090826HQ654894, KY051908
Xylariales sp.100.95MW090804 MW090816
MW090821
MW090822
MW090878		KC774617, KF415082
Zalerion sp.10.10MW090824AY465470, AF169308
Table
Table 3. Fungi colonies isolated from sapwood of bark-stripped Abies alba firs depending on wound type and habitation depth (O—outside part of wood; M—middle part of wood; H—the part near heartwood).
Table 3. Fungi colonies isolated from sapwood of bark-stripped Abies alba firs depending on wound type and habitation depth (O—outside part of wood; M—middle part of wood; H—the part near heartwood).
Fungal TaxaWoundControl
FreshHealedOld
OMHOMHOMHOMH
Alternaria alternata14696611112 5
Arthrinium arundinis1912943131476 3
Aureobasidium pullulans 1 3
Botrytis cinerea31 31
Cadophora sp. 6116
Chaetomium sp. 2
Coniochaeta sp. 1
Coprinellus micaceus 3
Cucurbitariaceae sp. 1
Cylindrobasidium evolvens766 232424
Cystobasidium laryngis 1
Cytospora sp.6661
Epicoccum nigrum9611 5 3
Eutypa sp. 514236
Fusarium acuminatum 6
Fusarium avenaceum 1
Fusarium tricinctum 36 12 3
Helotiales sp. 1
Heterobasidion annosum 1
Neonectria neomacrospora 5
Nigrospora oryzae 63
Paraphaeosphaeria neglecta266 611218121212
Penicillium chrysogenum 146 6
Peniophoraceae sp.
Phomopsis sp.71015 31372012 2
Preussia minima2 6
Rhizosphaera macrospora 4
Rhizosphaera oudemansii 1
Sarea difformis 5
Schizophyllum commune 126
Sordaria fimicola 1
Stereum sanguinolentum 6
Sydowia polyspora8485111
Tolypocladium sp. 11
Trametes versicolor 1 1
Trametes sp. 3
Trichoderma atroviride667424243687242730
Truncatella angustata2 3
Valsa sp. 1
Xylariales sp.1 111411
Zalerion sp. 1
In total:867093706379159157118435556
Table
Table 4. The number of isolated fungi colonies inhabiting wounds depending on the DBH of bark-stripped firs.
Table 4. The number of isolated fungi colonies inhabiting wounds depending on the DBH of bark-stripped firs.
Fungal TaxaDBH [cm]
4.0–6.97.0–9.910.0–12.913.0–16.9Control
Alternaria alternata4364125
Arthrinium arundinis23 19309
Aureobasidium pullulans 3 1
Botrytis cinerea4211
Cadophora sp. 1211
Chaetomium sp. 2
Coniochaeta sp.1
Coprinellus micaceus3
Cucurbitariaceae sp. 1
Cylindrobasidium evolvens3333186
Cystobasidium laryngis 1
Cytospora sp.18 1
Epicoccum nigrum7 6183
Eutypa sp.42243330
Fusarium acuminatum 6
Fusarium avenaceum 1
Fusarium tricinctum1 293
Helotiales sp.1
Heterobasidion annosum 1
Neonectria neomacrospora 5
Nigrospora oryzae9
Paraphaeosphaeria neglecta20 23648
Penicillium chrysogenum1 10 6
Peniophoraceae sp. 6
Phomopsis sp.171941282
Preussia minima 26
Rhizosphaera macrospora2 2
Rhizosphaera oudemansii1
Sarea difformis5
Schizophyllum commune 9
Sordaria fimicola1
Stereum sanguinolentum 6
Sydowia polyspora121015
Tolypocladium sp. 2
Trametes versicolor11
Trametes sp. 3
Trichoderma atroviride541339181
Truncatella angustata5
Valsa sp.1
Xylariales sp.12 7
Zalerion sp. 1
In total:306113215271172
Table
Table 5. α-diversity indexes values calculated per every type of sample. Abbreviations: J—Suhora forest district, Jasionów forest area, K—Kudłoń forest district, Za Palacem forest area, P—Kudłoń forest district, Pasieka forest area, S—Suhora forest district, Czarny Groń forest area.
Table 5. α-diversity indexes values calculated per every type of sample. Abbreviations: J—Suhora forest district, Jasionów forest area, K—Kudłoń forest district, Za Palacem forest area, P—Kudłoń forest district, Pasieka forest area, S—Suhora forest district, Czarny Groń forest area.
LocationType of WoundPart of Woodα-Diversity Indexes
Shannon-Wiener (H)Pielou (J)Simpson (D)
Joldoutside0.0000 0.0000
Jfreshinside0.41010.59170.2449
Jfreshoutside0.69020.99570.4970
Jfreshmiddle0.41010.59170.2449
Kcontrolinside0.98310.70910.5139
Kcontroloutside0.70780.64430.4224
Kcontrolmiddle1.06930.77130.5730
Koldinside1.67710.86180.7800
Koldoutside1.88210.85660.8180
Koldmiddle1.78730.85950.7956
Khealedinside0.62290.44930.3033
Khealedoutside0.42580.38760.2037
Khealedmiddle0.34880.50330.1975
Kfreshinside1.64080.91570.7846
Kfreshoutside1.45940.81450.7160
Kfreshmiddle0.69311.00000.5000
Poldinside0.0000 0.0000
Poldoutside0.20620.29750.0997
Poldmiddle0.0000 0.0000
Phealedinside2.27450.91530.8698
Phealedoutside1.90730.91720.8194
Phealedmiddle1.24250.89620.6667
Pfreshinside0.0000 0.0000
Pfreshoutside1.08890.78550.5800
Scontrolinside1.23090.88790.6746
Scontroloutside0.56230.81130.3750
Scontrolmiddle0.86990.79180.5128
Soldinside1.66270.85450.7756
Soldoutside1.72930.83160.7686
Soldmiddle1.89430.86210.8278
Shealedinside0.73560.66960.4063
Shealedoutside0.67300.97100.4800
Shealedmiddle0.63650.91830.4444
Sfreshinside2.05770.93650.8549
Sfreshoutside1.95680.89060.8375
Sfreshmiddle1.94370.93470.8443
Table
Table 6. Sørensen’s β-biodiversity index comparing the similarity of mycobiota inhabiting different types of bark-stripping wounds caused by red deer on silver fir in the Gorce National Park.
Table 6. Sørensen’s β-biodiversity index comparing the similarity of mycobiota inhabiting different types of bark-stripping wounds caused by red deer on silver fir in the Gorce National Park.
ControlFreshHealedOld
0.430.38
0.42
0.34
0.63
0.59
Table
Table 7. Survivability of young silver fir trees in the area of the Gorce National Park in a period 1992–2017 assessed on a net of 433 permanent monitoring plots (0.05 ha each) distributed in the whole area of the park (for methodology see e.g., Chwistek 2010). Abbreviations: C—man-cut trees, BW—broken and windthrown trees.
Table 7. Survivability of young silver fir trees in the area of the Gorce National Park in a period 1992–2017 assessed on a net of 433 permanent monitoring plots (0.05 ha each) distributed in the whole area of the park (for methodology see e.g., Chwistek 2010). Abbreviations: C—man-cut trees, BW—broken and windthrown trees.
DBH [d1,3; cm] in 1992Number of Silver Fir Trees in 1992Number of the Same Silver Fir Trees in 2017Mortality [N]Survival Rate [%]
7.0–7.916415311 (1C)93.3
8.0–8.91161106 (2C)94.8
9.0–9.91131103 (1C)97.3
10.0–10.971692 (1C)97.2
11.0–11.97170198.6
12.0–12.982802 (1BW)97.6
13.0–13.946451 (1BW)97.8
14.0–14.94039197.5
15.0–15.944440100
16.0–16.938380100
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MDPI and ACS Style

Pusz, W.; Baturo-Cieśniewska, A.; Kaczmarek-Pieńczewska, A.; Patejuk, K.; Czarnota, P. Is the Survivability of Silver Fir under Condition of Strong Ungulate Pressure Related to Mycobiota of Bark-Stripping Wounds? Forests 2021, 12, 976. https://doi.org/10.3390/f12080976

AMA Style

Pusz W, Baturo-Cieśniewska A, Kaczmarek-Pieńczewska A, Patejuk K, Czarnota P. Is the Survivability of Silver Fir under Condition of Strong Ungulate Pressure Related to Mycobiota of Bark-Stripping Wounds? Forests. 2021; 12(8):976. https://doi.org/10.3390/f12080976

Chicago/Turabian Style

Pusz, Wojciech, Anna Baturo-Cieśniewska, Agata Kaczmarek-Pieńczewska, Katarzyna Patejuk, and Paweł Czarnota. 2021. "Is the Survivability of Silver Fir under Condition of Strong Ungulate Pressure Related to Mycobiota of Bark-Stripping Wounds?" Forests 12, no. 8: 976. https://doi.org/10.3390/f12080976

APA Style

Pusz, W., Baturo-Cieśniewska, A., Kaczmarek-Pieńczewska, A., Patejuk, K., & Czarnota, P. (2021). Is the Survivability of Silver Fir under Condition of Strong Ungulate Pressure Related to Mycobiota of Bark-Stripping Wounds? Forests, 12(8), 976. https://doi.org/10.3390/f12080976

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