The E6 Oncoprotein of HPV16 AA-c Variant Regulates Cell Migration through the MINCR/miR-28-5p/RAP1B Axis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Screening of Differentially Expressed lncRNAs
2.2. Bioinformatic Analysis
2.3. Cell Culture and Transfection of siRNAs
2.4. RNA Extraction
2.5. RT-qPCR
2.6. Western Blot
2.7. Cell Migration Assays
2.8. Statistical Analysis
3. Results
3.1. The E6 Oncoprotein of the HPV16 AA-c Variant Increases the Expression Levels of lncRNA MINCR in Cells Derived from Cervical Cancer
3.2. lncRNA MINCR Expression Levels Correlate with miR-28-5p Levels
3.3. The Expression of miR-28-5p and RAP1B mRNA Is Altered in C33-A/AA-c Cells
3.4. The MINCR/miR-28-5p/RAP1B Axis Regulates Cell Migration in C33-A/AA-c Cells
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Perez-Bacho, E.G.; Beltrán-Anaya, F.O.; Arechaga-Ocampo, E.; Hernández-Sotelo, D.; Garibay-Cerdenares, O.L.; Illades-Aguiar, B.; Alarcón-Romero, L.D.C.; Del Moral-Hernández, O. The E6 Oncoprotein of HPV16 AA-c Variant Regulates Cell Migration through the MINCR/miR-28-5p/RAP1B Axis. Viruses 2022, 14, 963. https://doi.org/10.3390/v14050963
Perez-Bacho EG, Beltrán-Anaya FO, Arechaga-Ocampo E, Hernández-Sotelo D, Garibay-Cerdenares OL, Illades-Aguiar B, Alarcón-Romero LDC, Del Moral-Hernández O. The E6 Oncoprotein of HPV16 AA-c Variant Regulates Cell Migration through the MINCR/miR-28-5p/RAP1B Axis. Viruses. 2022; 14(5):963. https://doi.org/10.3390/v14050963
Chicago/Turabian StylePerez-Bacho, Eduardo Gil, Fredy Omar Beltrán-Anaya, Elena Arechaga-Ocampo, Daniel Hernández-Sotelo, Olga Lilia Garibay-Cerdenares, Berenice Illades-Aguiar, Luz Del Carmen Alarcón-Romero, and Oscar Del Moral-Hernández. 2022. "The E6 Oncoprotein of HPV16 AA-c Variant Regulates Cell Migration through the MINCR/miR-28-5p/RAP1B Axis" Viruses 14, no. 5: 963. https://doi.org/10.3390/v14050963
APA StylePerez-Bacho, E. G., Beltrán-Anaya, F. O., Arechaga-Ocampo, E., Hernández-Sotelo, D., Garibay-Cerdenares, O. L., Illades-Aguiar, B., Alarcón-Romero, L. D. C., & Del Moral-Hernández, O. (2022). The E6 Oncoprotein of HPV16 AA-c Variant Regulates Cell Migration through the MINCR/miR-28-5p/RAP1B Axis. Viruses, 14(5), 963. https://doi.org/10.3390/v14050963