The Importance of Poly(ethylene glycol) and Lipid Structure in Targeted Gene Delivery to Lymph Nodes by Lipid Nanoparticles
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Methods
2.2.1. Formulation of LNPs Containing pDNA
2.2.2. LNP Characterization
2.2.3. Analysis of Particle Stability
2.2.4. In vivo Gene Expression nLuc by LNPs
2.2.5. Ex vivo Whole-Organ Bioluminescence Imaging
2.2.6. NanoLuciferase (nLuc) Assay
2.2.7. Statistical Analysis
3. Results and Discussion
3.1. Synthesis of LNPs Encapsulating Plasmid DNA
3.2. Characterization of LNPs
3.3. Encapsulation Efficiency and Stability of LNPs
3.4. In Vivo Transfection of LNPs Stabilized by PEG-DSPE
3.5. In Vivo Transfection of LNPs Stabilized by Tween 80 and Tween 20
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Zukancic, D.; Suys, E.J.A.; Pilkington, E.H.; Algarni, A.; Al-Wassiti, H.; Truong, N.P. The Importance of Poly(ethylene glycol) and Lipid Structure in Targeted Gene Delivery to Lymph Nodes by Lipid Nanoparticles. Pharmaceutics 2020, 12, 1068. https://doi.org/10.3390/pharmaceutics12111068
Zukancic D, Suys EJA, Pilkington EH, Algarni A, Al-Wassiti H, Truong NP. The Importance of Poly(ethylene glycol) and Lipid Structure in Targeted Gene Delivery to Lymph Nodes by Lipid Nanoparticles. Pharmaceutics. 2020; 12(11):1068. https://doi.org/10.3390/pharmaceutics12111068
Chicago/Turabian StyleZukancic, Danijela, Estelle J. A. Suys, Emily H. Pilkington, Azizah Algarni, Hareth Al-Wassiti, and Nghia P. Truong. 2020. "The Importance of Poly(ethylene glycol) and Lipid Structure in Targeted Gene Delivery to Lymph Nodes by Lipid Nanoparticles" Pharmaceutics 12, no. 11: 1068. https://doi.org/10.3390/pharmaceutics12111068
APA StyleZukancic, D., Suys, E. J. A., Pilkington, E. H., Algarni, A., Al-Wassiti, H., & Truong, N. P. (2020). The Importance of Poly(ethylene glycol) and Lipid Structure in Targeted Gene Delivery to Lymph Nodes by Lipid Nanoparticles. Pharmaceutics, 12(11), 1068. https://doi.org/10.3390/pharmaceutics12111068