The First Report on Saprolegnia parasitica and Neoparamoeba perurans Isolated from Atlantic Salmon (Salmo salar) Reared in Korea
Round 1
Reviewer 1 Report
Comments and Suggestions for Authors
Case Report
The first report on Saprolegnia parasitica and Neoparamoeba perurans isolated from Atlantic salmon (Salmo salar), reared in Korea
It's a very interesting paper. I learned a lot of things about fungal and amoebal infections of fish.
This study represents the report of S. parasitica and N. perurans detected in Atlantic salmon, a species in which aquaculture technology research is actively progressing in Korea. Based on this study, it is crucial to promptly isolate the pathogens and quarantine infected fish by identifying the morphological characteristics of S. parasitica and N. perurans and the external symptoms of Atlantic salmon infected with these pathogens. In addition to these efforts, it is necessary to develop disease control measures to establish the domestic Atlantic salmon aquaculture industry in Korea successfully.
The part M and M
Must be written in details with all available information, please,
International literature
It seems to me that a lot of information are not taken in consideration. Looking in the pubmed, I found a lot of reviews with 2 very significant papers.
A review of the current status of Cryptosporidium in fish.
Cryptosporidium in fish: Implications for aquaculture and beyond.
References
Pl check and edit based on the journal’s guidelines
Language: although well written, I strongly recommend to improve the language style, expression and edits for transparency and clarity of the language.
Further issues:
Summary:
Comment 1. P.1 L.27. “fuse” – “fused”
Introduction:
Comment 2. P.1, L. 40. Please add the scientific name of Atlantic salmon for the first time.
Commen 3. P. 2, L.44. “disease” – “diseases”
Comment 4. P. 2, L. 50. “(bacteria, viruses, parasites, and fungal)” – “(bacterial, viral, parasitic, and fungal)
Comment 5. P.2, L.65. “condition” – “conditions”
Comment 6. P.2, L.70. Please add the scientific name of this species: rainbow trout, and coho salmon.
Comment 7. Please add the classification of Neoparamoeba perurans. Because of there are classification of Saprolegnia parasitica.
Materials and Methods:
Comment 8. P. 2 and 3. In March 2023 and in August 2023, freshwater and seawater temperature was 13°C, was the same temperature of water in March and August? In our country, it will not be real. About Korea I can’t say nothing.
Comment 9. P.3, L.114. “fungal disease” – “the fungal disease”
Comment 10. P. 3, L.122. “parasitic disease” – “the parasitic disease”
Commen 11. P. 3, L. 133. “primers and specific for Neoparamoeba species” – “primers specific for Neoparamoeba species”
Comment 12. P.3, L. 136. “alignment” – “alignments”
Histopathological analysis
Comment 13. P3. L. 141-145. No need to repeat this sentence twice: They were then fixed in 10% neutral-buffered formalin solution (Sigma, USA) for 24 hours.
You can write:
For fungal diseases, samples were collected from the gills, gill covers, fins, brain, liver, intestine, muscle, spleen, kidney, and clinically symptomatic areas of Atlantic salmon. For parasitic diseases, samples were collected from the gills and clinically symptomatic areas of Atlantic salmon. They were then fixed in 10% neutral-buffered formalin solution (Sigma, USA) for 24 hours.
Comment 14. P.4, L. 160. “fungal disease” – “the fungal disease”
Comment 15. P. 4, L.175. “identify” – “identification”
Comment 16. P.7, L. 224. “together” – “to each other”
Discussion:
Comment 17. P.7, L.228-231. I think this phrase is belonging to introduction.
Comment 18. P.8, L.308-313. I think this phrase is belonging to conclusions.
Not matching reference links:
Comment 19.P.8, L.292. Please check the 49-th reference:
S. parasitica has been reported to penetrate the epithelium, causing epithelial damage and cell necrosis and to penetrate the muscle, leading to hemorrhage and inflammation [48,49].
49. Kulatunga, D.C.; Dananjaya, S.H.; Park, B.K.; Kim, C.H.; Lee, J.; DeZoysa, M. First report of Fusarium oxysporum species complex infection in zebrafish culturing system. J. Fish. Dis. 2017, 40, 485-494. https://doi.org/10.1111/jfd.12529
Comment 20.P.8, L.292. Please check the 67-th reference:
The morphological characteristics of the isolated N. perurans observed under the microscope showed a lack of surface structures, with round or hexagonal trophozoites of white color and the presence of a nucleus and parasome (Figure 4B, 5D), which were similar to previous research findings [35,67,68]
67. Page, F.C. The classification of the ‘naked’ amoebae of the (Phylum Rhizopoda). Arch. Protistenkd. 1987, 133, 199-217. https://doi.org/10.1016/S0003-9365(87)80053-2
Comments on the Quality of English LanguageGood
Improvements are highly recommended
Author Response
Comment 1. P.1 L.27. “fuse” – “fused”
-> I have made modifications
Comment 2. P.1, L. 40. Please add the scientific name of Atlantic salmon for the first time.
-> I have made modifications
Commen 3. P. 2, L.44. “disease” – “diseases”
-> I have made modifications
Comment 4. P. 2, L. 50. “(bacteria, viruses, parasites, and fungal)” – “(bacterial, viral, parasitic, and fungal)
-> I have made modifications
Comment 5. P.2, L.65. “condition” – “conditions”
-> I have made modifications
Comment 6. P.2, L.70. Please add the scientific name of this species: rainbow trout, and coho salmon.
->I have added the scientific name
Comment 7. Please add the classification of Neoparamoeba perurans. Because of there are classification of Saprolegnia parasitica.
->I have added the classification for N. perurans.
Comment 8. P. 2 and 3. In March 2023 and in August 2023, freshwater and seawater temperature was 13°C, was the same temperature of water in March and August? In our country, it will not be real. About Korea I can’t say nothing.
->Using a chiller, the water temperature is maintained at 13°C.
Comment 9. P.3, L.114. “fungal disease” – “the fungal disease”
-> I have made modifications
Comment 10. P. 3, L.122. “parasitic disease” – “the parasitic disease”
-> I have made modifications
Commen 11. P. 3, L. 133. “primers and specific for Neoparamoeba species” – “primers specific for Neoparamoeba species”
-> I have made modifications
Comment 12. P.3, L. 136. “alignment” – “alignments”
-> I have made modifications
Comment 13. P3. L. 141-145. No need to repeat this sentence twice: They were then fixed in 10% neutral-buffered formalin solution (Sigma, USA) for 24 hours.
-> I have made modifications
Comment 14. P.4, L. 160. “fungal disease” – “the fungal disease”
-> I have made modifications
Comment 15. P. 4, L.175. “identify” – “identification”
-> I have made modifications
Comment 16. P.7, L. 224. “together” – “to each other”
-> I have made modifications
Comment 17. P.7, L.228-231. I think this phrase is belonging to introduction.
-> I have deleted the content.
Comment 18. P.8, L.308-313. I think this phrase is belonging to conclusions.
-> I have deleted the content.
Not matching reference links:
Comment 19.P.8, L.292. Please check the 49-th reference:
-> I have made modifications
Comment 20.P.8, L.292. Please check the 67-th reference:
-> I have made modifications
Author Response File: 
Author Response.pdf
Reviewer 2 Report
Comments and Suggestions for AuthorsThe paper titled The first report on Saprolegnia parasitica and Neoparamoeba perurans isolated from Atlantic salmon (Salmo salar), reared in Korea gives novel information on the presence of fungus and parasite infections in salmon in Korea. The paper is worth publishing after major revision of the following:
1- English Editing
2- Correction of Italics like Scientific names and in vitro and others
3- Is there any relation between the two infections in the salmon the author noted or in previous studies found any where in the world if yes please clarify in the introduction and discussion, if not no problem.
4- Add the approval number of the study in M&M.
5- References need keen and strong check with journal guidelines and journal names many are not correct or not correctly abbreviated please check—also the titles of the references. Why did the author add a dot after a non-abbreviated name please, remove these dots.
6-I noted some mistakes in yellow in the pdf attached. Please, check all the paper not only these yellow ones.
Comments for author File: Comments.pdf
English editing is required.
Author Response
1- English Editing
-> I have made some modifications
2- Correction of Italics like Scientific names and in vitro and others
-> I have made some modifications
3- Is there any relation between the two infections in the salmon the author noted or in previous studies found any where in the world if yes please clarify in the introduction and discussion, if not no problem.
-> Research on the issues arising between these two infections has not yet been conducted, and it has not been found in existing studies either. Additionally, S. parasitica was isolated from freshwater, while N. perurans was isolated from seawater, suggesting that each infection occurs separately.
4- Add the approval number of the study in M&M.
-> I have added the approval number
5- References need keen and strong check with journal guidelines and journal names many are not correct or not correctly abbreviated please check—also the titles of the references. Why did the author add a dot after a non-abbreviated name please, remove these dots.
-> I have made some modifications
6-I noted some mistakes in yellow in the pdf attached. Please, check all the paper not only these yellow ones.
-> I have revised and confirmed the sentence.
Author Response File: Author Response.pdf
Round 2
Reviewer 1 Report
Comments and Suggestions for Authorsmy comments have been answered
Comments on the Quality of English LanguageAll is fine now.
