Phosphorus Mobilization in Plant–Soil Environments and Inspired Strategies for Managing Phosphorus: A Review
, Sardar Khan 5, Dong-Xing Guan 6 and Gang Li 1,7,8,*
Round 1
Reviewer 1 Report
My suggestions for authors are:
1 - when they want to write a review, it should be tackle a problem or issue in new ways.
2- At review, the hypotheses and objectives should be cleared and matched.
3- It should be showing data that matched with the review written purpose.
Author Response
Point 1: When they want to write a review, it should be tackle a problem or issue in new ways
Response 1: Thank you for your good comments. We have revised the manuscript and hopefully, the quality is improved now.
Point 2: At review, the hypotheses and objectives should be cleared and matched
Response 2: We have revised the manuscript and hopefully, there is a clear hypothesis and objectives.
Point 3: It should be showing data that matched with the review written purpose.
Response 3: We have rearranged the data and hope it has a clear purpose now.
Reviewer 2 Report
a comprehensive work, please note the comments in the file;
For the results in Table 3, a description of the experimental setup and soil properties, P content before application (possibly CK) is require
Comments for author File: 
Comments.pdf
Author Response
Point 1: For the results in Table 3, a description of the experimental setup and soil properties, P content before application (possibly CK) is require.
Response 1: The tested soil had a pH of 6.52 units and a total P concentration of 5.59 mg kg-1. The Incubation experiment was conducted at a lab scale in a complete randomized block design (CRBD) in four replicates. Each carrier was amended separately at the rate of 3 % to the tested soil. After one month of incubation carrier RSB and SSB, amendment showed significantly increased concentration of 0.5 M NaHCO3 Extractable (Pi and Po), 0.1M NaOH Extractable (Pi and Po), 1MHCl (Pi), Residual P, and total P in applied soil as compared with RHB, PNB, WB, CCB and CK after sequential extraction.
Point 2: Do you mean Mg and not mg (Megagramm and not milligramm)
Response 2: I mean Mg (Megagramm).
Point 3: Is this correct?
Response 3: No it was not correct we delete it.
Point 4: Is there a test design or literature source for this?.
Response 4: The Incubation experiment was conducted at a lab scale in a complete randomized block design (CRBD) in four replicates. Each carrier was amended separately at the rate of 3 % to the tested soil.
Point 5: Table 2?
Response 5: No it is Table 3.
Point 6: The techniques are the same! You can omit the table and just mention the soils and references in the text, also the DGT technique should be described briefly.
Response 6: We delete the techniques DGT column and briefly described DGT technique.
Point 6: Not in the reference list.
Response 7: Citation references are now included in the reference list
Reviewer 3 Report
Thanks much for invitation to review the manuscript “Phosphorus Mobilization Mechanism in Plant Soil Environment and Inspired Strategies for Managing Phosphorus in Agricultural Soil. A Review” It could be consider in the present journal after the incorporation the following suggestion.
The suggestion are as under
## The title need to be concise Phosphorus Mobilization Mechanism in Plant Soil Environment and Inspired Strategies for Managing Phosphorus in Agricultural Soil. A Review
##rearrange the sentence clear meaning This article systemically reviewed the mechanism of P uptake and P use efficiency by plants in starvation conditions. In addition, recent progress made in imaging techniques and different strategies used for improving plants available was identified. P mobilization and imaging techniques in a plant-soil environment were critically discussed. P use efficiency was reviewed from the aspect of how plants fulfill P requirement from tissues stored P during the status of P starvation. Following this understanding, different strategies to improve plant available P in agricultural soil were reviewed.
## Basic chemistry of P is missing phosphorus (P) is a fundamental macronutrient required for optimum plant growth and development in the agricultural sector [1]. P non-availability is a serious issue and is considered the main limiting factor for decreased crop yield in the modern agriculture ecosystem, particularly in developing countries [2]. In acidic soil, P is mostly immobi- lized in two forms. It may be trapped with mineral compounds of iron (Fe) or aluminum(Al) hydroxides or can be incorporated into the rocks rich mineral oxides such as hema- tite, goethite, and gibbsite [3].
## Colour the different component Fig 1: Mechanism of P uptake from soil to plant and associated biochemical process in the plant- 20
soil environment.
## very limited information has been discussed 4.5. Phosphate solubilizing microorganism application
## Add more information Table 1. Different amended/inoculation methods used and concentration of available and total P 7
(mg kg-1) in.agricultural soil.
## Citationstyle not properTable 2. Imaging techniques used in the measurement of P in different plants rhizosphere.
# Fig 3: Inspired strategies for managing Phosphorus and its fractionation Put the fig at their own place not at the bottem
Author Response
Point 1: Thanks much for invitation to review the manuscript “Phosphorus Mobilization Mechanism in Plant Soil Environment and Inspired Strategies for Managing Phosphorus in Agricultural Soil. A Review” It could be consider in the present journal after the incorporation the following suggestion.
Response 1: Dear Reviewer: We thank you for your valuable comments and the time you spent on our paper. We have tried our best to revise the paper according to your comments and hopefully, the paper quality is improved now.
Point 2: The title need to be concise Phosphorus Mobilization Mechanism in Plant Soil Environment and Inspired Strategies for Managing Phosphorus in Agricultural Soil. A Review.
Response 2: We have revised the title “Phosphorus Mobilization in Plant Soil Environment and Inspired Strategies for Managing Phosphorus. A Review.
Point 3: Rearrange the sentence clear meaning. This article systemically reviewed the mechanism of P uptake and P use efficiency by plants in starvation conditions. In addition, recent progress made in imaging techniques and different strategies used for improving plants available was identified. P mobilization and imaging techniques in a plant-soil environment were critically discussed. P use efficiency was reviewed from the aspect of how plants fulfill P requirement from tissues stored P during the status of P starvation. Following this understanding, different strategies to improve plant available P in agricultural soil were reviewed.
Response 3: The sentences are rearranged and hopefully it has a clear meaning now.
The mechanism of P uptake and P use efficiency by plants in starvation conditions was reviewed systemically. Furthermore, recent advances in imaging techniques and various strategies for increasing plant available P were identified. P use efficiency was examined in terms of how plants fulfill P requirements from tissues stored P during P starvation. Following this understanding, various strategies for increasing plant-available P in agricultural soil were reviewed. Finally, an update on the novel carrier used to improve the P content of agricultural soil was provided.
Point 4: Basic chemistry of P is missing phosphorus (P) is a fundamental macronutrient required for optimum plant growth and development in the agricultural sector [1]. P non-availability is a serious issue and is considered the main limiting factor for decreased crop yield in the modern agriculture ecosystem, particularly in developing countries [2]. In acidic soil, P is mostly immobi- lized in two forms. It may be trapped with mineral compounds of iron (Fe) or aluminum(Al) hydroxides or can be incorporated into the rocks rich mineral oxides such as hema- tite, goethite, and gibbsite [3].
Response 4: Basic chemistry of P is provided now and hopefully the quality is improved now.
As a non-metallic element in the periodic table's nitrogen group family, P was discovered in 1669 by Hennig Brandt, a German merchant. It is one of the 12 most abundant elements in the earth's crust. The only isotope that occurs naturally is 31P.
Point 5: Colore the different component Fig 1: Mechanism of P uptake from soil to plant and associated biochemical process in the plant- 20 soil environment.
Response 5: We have colored different components of Fig 1. Hopefully, the quality is improved now.
Point 6: Very limited information has been discussed 4.5. Phosphate solubilizing microorganism application.
Response 6: More information has been provided about phosphate solubilizing microorganism application.
Phosphorus solubilization is also influenced by soil texture. Aluminum (Al) and iron (Fe) chemical reactions with P in acidic or low pH soil result in P reduction. However, tricalcium phosphate (Ca3PO4)3 is formed in alkaline or high soil pH, reducing plant available P in soil [100]. Thus, soil alkalinity causes P unavailability, a process known as P fixation. Sorghum yield increased significantly after PSM inoculation [101]. To increase P availability, bacterial species such as Bacillus spp., Pseudomonas spp., and Agrobacterium spp. are used as soil inoculants [102]. Phosphorus solubilization, which involves local acidification or alkalization, has been observed in some Pseudomonas, Cyanobacteria, and Bacillus species isolated from plant rhizospheres [103]. Organic phosphate is the most abundant source of soil phosphate, but its compounds are complex (nucleic acids, phospholipids, etc.) and must be transformed by microorganisms before they can be absorbed by plants [104].
Point 7: Add more information Table 1. Different amended/inoculation methods used and concentration of available and total P 7 (mg kg-1) in.agricultural soil.
Response 7: We have added more information in Table 1. Now it is Table 2.
Point 8: Citation style not proper Table 2. Imaging techniques used in the measurement of P in different plants rhizosphere.
Response 8: We have arranged citations in proper style in Table 2 and now it is Table 1 Imaging techniques used in the measurement of P in different plant’s rhizosphere.
Point 9: Fig 3: Inspired strategies for managing Phosphorus and its fractionation Put the fig at their own place not at the bottem.
Response 9: Fig 3 is changed to Fig 2 and is placed in the proper place.
Round 2
Reviewer 1 Report
I have read this review, it is the second time. However, there is a misleading on this review writing. My suggestions for authors are adding more results for each section that been worked by other researchers. Also, delete so many sentences or facts that been repeated on this review. Some sections look like a repeated work with no any ideas or information in. Otherwise on that, this review is good for a book chapter after adding many sentences. For now, it is not a review paper and it needs some work.
I attached a copy that has all my comments and suggestions.
Comments for author File: Comments.pdf
Author Response
Point 1: I recommende you to write the other issues regarding P. Eventhough, you mention few things about P, but introduction is still unclear, unmatached sentences, you have to rewrite this section.
Response 1: Thank you for your good comments. We have revised the manuscript and hopefully, the quality is improved now.
Point 2: Delete this.
Response 2: We have deleted the sentence “As a non-metallic element in the periodic table's nitrogen group family”, and hopefully, the quality is improved now.
Point 3: Dont begin the sentences with abbreviations everywhere in this review.
Response 3: We have used the full name of Phosphorus.
Point 4: 31P
Response 4: we used the full name Phosphorus-31 (31P).
Point 5: Everywhere
Response 5: we have included everywhere.
Point 6: It is not clear. which forms? this sentence needs to be clearfy.
Response 6: In acidic soil, soil P is mostly immobilized in two forms i.e. inorganic phosphorus (Pi) and organic phosphorus (Po).
Point 7: Write down which insoluble compounds.
Response 7: variscite, strengite, and apatite
Point 8: write the whole word.
Response 8: Organic phosphorus
Point 9: You should mean pi
Response 9: Pi
Point 10: What are they? mention them here.
Response 10: texture, organic matter, and electric conductivity
Point 11: Write which forms?
Response 11: inositol phosphates, phospholipids, and sugar phosphates
Point 12: Write soil pH.
These ranges are wrong. you have to make sure that which right ranges. when h2po4 and hpo4 will be available?
Response 12: pH range 6.0-6.5
Point 13: This sentence is confusing.
Response 13: the sentence is corrected now
Point 14: inorganic
Response 14: chemical
Point 15: fertile
Response 15: Fertility was removed.
Point 16: Which kind of soil that p can be leached?
Response 16: fertilizer applied soil
Point 17: It is not clear. you have to clearfy this here what does it mean?
Response 17: due to risks of underground water contamination.
Point 18: P fertilizers
Response 18: Chemical fertilizer
Point 19: Depends on how much was applied. Not all regions or soils that p can be leached from.
Response 19: we have removed the sentence.
Point 20: Change this word.
Response 20: declined.
Point 21: What does it mean?
Response 21: the sentence is revised by the addition of by P supplement from bulk soil
Point 22: At the sentences beginning write the whole name
Response 22: We have written the whole name.
Point 23: What do you mean by this table?
Response 23: Publications on diffusive gradients in thin films during 2012-2021
Point 24: What does Pt include? you should clarify these forms.
Response 24: composed of inorganic phosphorus (Pi) and organic phosphorus (Po).
Point 25: It is a big mistake, check the right names.
Response 25: H2 PO4- and HPO4-2
Point 26: It is not clear enough what are you trying to say by this. your sentences are not sequenced.
Response 26: we have revised.
Point 27: H2PO4 and HPO4, it should be corrected everywhere in this review.
Response 27: we have corrected.
Point 28: This figure is not clear and is not telling anything. However, at the caption, clarify all abbreviations that been written in this fig.
Response 28: we have revised. Hopefully is cleared now.
Point 29: Which applications? We do have so many applications such as the chemicals, manure, and bio applications. Each one of these applications has a different process into the soil.You have to clarify each one apart.
In this section you should say the chemical P fertilizers.
Response 29: we have revised.
Point 30: ?
Response 30: we have corrected.
Point 31: How it can do this? You should write the whole idea.
Response 31: we have written the whole idea.
Point 32: Which kind of pathways? You have to explain what are they more than what has been written.
Response 33: Mycorrhiza plants use two pathways (direct, and mycelium)
Point 34: Not just P but also N.
Response 34: chemical
Point 35: The PGPB….
Response 35: The plant growth promoting bacteria
Point 36: This section is repeated and the same above section. however, merge this section with 4.4 section and exclude the repeated sentences facts.
Response 36: we have merged and removed the repeated sentences.
Point 37: PSM
Response 37: Phosphate solubilizing microorganism
Point 38: Where are the micro nutrients?
Response 38: copper (Cu), zinc (Zn), iron (Fe), and manganese (Mn)
Point 39: PGPB
Response 39: Plant growth promoting bacteria
Point 40: Total phosphorus is more than this.
Response 40: we searched but don’t find more than this.
Point 41: Please check this.
Response 41: an orthophosphate ion H2PO4-, and HPO4-2
Point 42: What was soil test p merthod ?
Response 42: to Mo-Blue method (Murphy and Riley, 1962).
Point 43: Conclusion have to rewrite. It is not a conclusion.
Response 43: we have revised the conclusion.
Reviewer 3 Report
Dear sir,
The paper can be accept in its present form as I am satisfied with the correction.
Author Response
Point 1: Dear sir, The paper can be accept in its present form as I am satisfied with the correction.
Response 1:. Thank you for your nice comments and the time you have given to our review.
