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Article

Brain Metabolomics in Fragile X-Associated Tremor/Ataxia Syndrome (FXTAS)

by
Maria Jimena Salcedo-Arellano
1,2,3,
Michael D. Johnson
1,3,
Yingratana A. McLennan
1,3,
Ye Hyun Hwang
4,
Pablo Juarez
1,3,
Erin Lucille McBride
1,3,
Adriana P. Pantoja
1,3,
Blythe Durbin-Johnson
5,
Flora Tassone
2,4,
Randi J. Hagerman
2,6 and
Verónica Martínez-Cerdeño
1,2,3,*
1
Department of Pathology and Laboratory Medicine, UC Davis School of Medicine, Sacramento, CA 95817, USA
2
Medical Investigation of Neurodevelopmental Disorders (MIND) Institute, University of California Davis, Sacramento, CA 95616, USA
3
Institute for Pediatric Regenerative Medicine at Shriners Hospitals for Children Northern California, Sacramento, CA 95817, USA
4
Department of Biochemistry and Molecular Medicine, UC Davis School of Medicine, Sacramento, CA 95817, USA
5
Division of Biostatistics, Department of Public Health Sciences, UC Davis School of Medicine, Sacramento, CA 95817, USA
6
Department of Pediatrics, UC Davis School of Medicine, Sacramento, CA 95817, USA
*
Author to whom correspondence should be addressed.
Cells 2023, 12(17), 2132; https://doi.org/10.3390/cells12172132
Submission received: 27 May 2023 / Revised: 8 August 2023 / Accepted: 15 August 2023 / Published: 23 August 2023
(This article belongs to the Special Issue The Molecular Basis of Fragile X Syndrome and Other FMR1-Associated Conditions)
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Abstract

:
The course of pathophysiological mechanisms involved in fragile X-associated tremor/ataxia syndrome (FXTAS) remains largely unknown. Previous proteomics and metabolomics studies conducted in blood samples collected from FMR1 premutation carriers with FXTAS reported abnormalities in energy metabolism, and precursors of gluconeogenesis showed significant changes in plasma expression levels in FMR1 premutation carriers who developed FXTAS. We conducted an analysis of postmortem human brain tissues from 44 donors, 25 brains with FXTAS, and 19 matched controls. We quantified the metabolite relative abundance in the inferior temporal gyrus and the cerebellum using untargeted mass spectrometry (MS)-based metabolomics. We investigated how the metabolite type and abundance relate to the number of cytosine-guanine-guanine (CGG) repeats, to markers of neurodegeneration, and to the symptoms of FXTAS. A metabolomic analysis identified 191 primary metabolites, the data were log-transformed and normalized prior to the analysis, and the relative abundance was compared between the groups. The changes in the relative abundance of a set of metabolites were region-specific with some overlapping results; 22 metabolites showed alterations in the inferior temporal gyrus, while 21 showed differences in the cerebellum. The relative abundance of cytidine was decreased in the inferior temporal gyrus, and a lower abundance was found in the cases with larger CGG expansions; oleamide was significantly decreased in the cerebellum. The abundance of 11 metabolites was influenced by changes in the CGG repeat number. A histological evaluation found an association between the presence of microhemorrhages in the inferior temporal gyrus and a lower abundance of 2,5-dihydroxypyrazine. Our study identified alterations in the metabolites involved in the oxidative-stress response and bioenergetics in the brains of individuals with FXTAS. Significant changes in the abundance of cytidine and oleamide suggest their potential as biomarkers and therapeutic targets for FXTAS.

1. Introduction

Fragile X-associated tremor/ataxia syndrome (FXTAS) is a late-onset single-gene neurodegenerative disorder characterized by the following core clinical symptoms: balance problems (ataxia), intention tremor, and cognitive decline. FXTAS is caused by an expanded trinucleotide repeat in the promoter region of the FMR1 (fragile X messenger ribonucleoprotein 1) gene that codes for a crucial protein, FMRP, important for cognitive development. Not all individuals who are FMR1 premutation carriers (expansions between 54 and 200 CGG repeats) will go on to develop FXTAS, but the conversion rate increases with age, with the average diagnosis age being around 61 years in males and 68 years in females. Premutation-carrier females have one normal X allele that helps compensate for the expanded one and thus have milder symptoms and a lower FXTAS penetrance rate. Approximately 40% of males and 16% of females develop FXTAS. An added layer of complexity is that some premutation-carrier individuals have mild symptoms; this phenotype is commonly seen in females who were originally thought to be unaffected, while others have symptoms that emerge rapidly and get worse over time.
There is no single and simple test for FXTAS. The sensitivity and specificity of clinical diagnosis methods are low and only effective in patients with significant core symptoms. Magnetic resonance imaging of the brain supports the diagnosis of FXTAS; however, white matter hyperintensities in the middle cerebellar peduncles (MCP sign), a major radiological criterion, are seen in approximately 60% of males and in <13% of females. The pathological examination of postmortem brain is widely accepted as the final diagnostic method of FXTAS by confirming eosinophilic-ubiquitin-positive intranuclear inclusions [1,2]. Like other diseases, an effective drug treatment may be needed before the onset of clinical symptoms. In this context, metabolomics can be a useful tool to detect altered metabolites that are closely associated with FXTAS pathogenesis. Similar to most neurodegenerative disorders with no known cure, there is an urgent need for biomarkers that can facilitate the development, diagnosis, severity, prognosis, and treatment of FXTAS.
Primary metabolites, products of the metabolism that are directly involved in cell maintenance, growth, and reproduction, are emerging substances that can be used as potential disease biomarkers. An imbalance of metabolic homeostasis is a precursor for disease, so the assessment of changes in the metabolome through metabolomics analysis has become an important application for understanding the complexity of a disease phenotype and for discovering novel therapeutic targets. Previous metabolomics studies conducted in human plasma and brain tissue from animal models showed metabolic differences between FMR1 premutation with and without FXTAS and controls [3,4,5,6].
Here, we report the metabolomic profile of postmortem brain tissues from patients who died from FXTAS and report the pathways potentially affected by FXTAS pathophysiology [7]. We employed an untargeted metabolomics approach using a combination of gas chromatography coupled with mass spectrometry (GC-MS) for a global metabolic profile of the postmortem human brain cortex and cerebellum (CB) from individuals with FXTAS. This approach accurately quantified 513 primary metabolites, with 191 being identified, from 44 tissue samples harvested from the inferior temporal gyrus (ITG) and CB. We performed a pathway enrichment analysis to detect altered biological processes and a receiver operator characteristic (ROC) curve analysis on a selected list of metabolites to assess for their discriminatory potential. These findings can potentially help to identify the key predictive biomarkers of FXTAS and expand the basic knowledge of the metabolome. Understanding the metabolic processes associated with core clinical symptoms in FXTAS may aid in uncovering biochemical pathways associated with different FXTAS phenotypes.

2. Materials and Methods

2.1. Participants

Twenty-five brains with FXTAS and nineteen matched controls for age and sex were included in this study (Table 1). FXTAS cases were obtained from the FXS/FXTAS brain repository at UC Davis (a node of the Hispanic-American Brain Bank for Neurodevelopmental Disorders—CENE) [8]. All donors gave written informed consent for a brain autopsy and the use of the material and clinical information for research purposes. Control brain tissues were obtained from the NIH NeuroBioBank and acquired from subjects without any significant neurological history or the FMR1 premutation. FXTAS cases were advanced in the disease progression and had a variable clinical presentation. FXTAS diagnosis was confirmed with the presence of the FMR1 premutation and postmortem ubiquitin-positive intranuclear inclusions in brain cells.

2.2. GC-MS Sample Preparation and Metabolite Profiling

Five hundred milligrams (0.5 g) of fresh frozen brain tissue were sampled from the ITG (Brodmann area 20) and the CB. Samples were stored at −80 degrees Celsius prior to analysis. Metabolite relative abundance was conducted by the West Coast Metabolomics Center using GC-MS quantification. Samples were extracted using 1 mL of 3:3:2 ACN:IPA:H2O (v/v/v). Half of the sample was dried to completeness and then derivatized using 10 µL of 40 mg/mL methoxyamine in pyridine. The samples were shaken at 30 °C for 1.5 h. Then, 91 µL of N-methyl-N-(trimethylsilyl) trifluroacetamide (MSTFA) + fatty acid methyl esters (FAMEs) were added to each sample, and they were shaken at 37 °C for 0.5 h to finish derivatization. The samples were then vialed, capped, and injected onto the instrument. Data were acquired using the chromatographic parameters detailed in Fiehn O. et al. [9]. Metabolite profiling was performed by an ALEX-CIS GC TOF MS system from the Gerstel corporation (Linthicum, MD, USA). Chromatographic parameters were as follows: column, Restek corporation Rtx-5Sil MS (30 m length × 0.25 mm internal diameter with 0.25 µm film made of 95% dimethyl/5%diphenylpolysiloxane); mobile phase, helium; column temperature, between 50–330 °C; flow rate, 1 mL min−1; injection volume, 0.5 µL; injection, 25 splitless time into a multi-baffled glass liner; injection temperature, 50 °C ramped to 250 °C at 12 °C s−1. The oven temperature parameters were as follows: 50 °C for 1 min, then ramped at 20 °C min−1 to 330 °C and held constant for 5 min. The mass spectrometry parameters were as follows: a Leco Pegasus IV mass spectrometer (St. Joseph, MI, USA) was used with a unit mass resolution at 17 spectra s−1 from 80–500 Da at a −70 eV ionization energy and an 1800 V detector voltage with a 230 °C transfer line and a 250 °C ion source. Data analysis was achieved using ChromaTOF vs. 2.32 (St. Joseph, MI, USA). Signal intensities were reported as peak heights using the unique ion as default. Raw data were submitted to BinBase (West Coast Metabolomics Center, Davis, CA, USA). Data were normalized using sum normalization after BinBase data processing during data curation. A series of 13 FAMEs were used as internal standards for the retention index and QC during acquisition to control for batch variation. All metabolomics data generated, in both raw and processed formats, and total ion chromatograms are available at the Metabolomics Workbench www.metabolomicsworkbench.org (accessed on 22 August 2023).

2.3. CGG Repeat Length

CGG allele repeat size was obtained from DNA samples isolated from 3 mL of whole blood using the standard procedure (Qiagen, Valencia, CA, USA) and assessed through polymerase chain reaction (PCR) and Southern blot analysis as previously described [10,11]. PCR used specific FMR1 primers, and PCR products were visualized by capillary electrophoresis and analyzed with the Peak Scanner Software 2.0 (Thermo Fisher Scientific, Waltham, MA, USA).

2.4. Markers of Neurodegeneration

We analyzed the presence/absence of amyloid beta plaques, tau aggregates (neurofibrillary tangles and neuritic plaques), and microhemorrhages, all of which are markers of neurodegenerative processes in the brain. Emerging evidence points to the co-existence of Alzheimer’s-type pathology in the brain cortex and the presence of microhemorrhages in the cerebellum as possible aggravating factors for the rate of disease progression in FXTAS [12,13,14]. Fixed samples from the ITG (Brodmann area 20) and CB were immersed in 30% sucrose and embedded in an optimal cutting temperature compound. Afterwards, each sample block was cut on the cryostat at a 14 μm thickness. We incubated sections in DIVA for 8 minutes at 110 °C followed by 3% hydrogen peroxide, permeabilized and blocked them in a tris-buffered saline (TBS) solution containing Triton and donkey serum for 1 hour (75% TBS, 15% Triton, 10% serum), and incubated them with the following primary antibodies overnight at 4 °C in a dark and humid box: rabbit anti-ubiquitin (1:150; Dako, Glostrup, Denmark), polyclonal rabbit anti-β amyloid 1–42 (1:500; Abcam, Cambridge, UK), and mouse monoclonal anti-phosphorylation clone AT8 tau (1:200, Invitrogen, Waltham, MA, USA). On day 2, sections were incubated with biotinylated secondary antibody (1:150, Jackson ImmunoResearch, West Grove, PA, USA) for 1 hour, incubated in a Vectastain ABC kit (Vector Labs, Burlingame, CA, USA) for 2 h, developed in a DAB kit (Vector Labs, Burlingame, CA, USA), dehydrated with alcohols, cleared in xylene, and cover slipped. Immunochemistry was used to evaluate the presence/absence of neurofibrillary tangles, neuritic plaques, and FXTAS intranuclear inclusions. Hematoxylin- and eosin-stained tissue was evaluated for the presence of microhemorrhages, defined as small (<10 mm), cortical/subcortical perivascular hemorrhages.

2.5. Symptoms of FXTAS

We extracted clinical data from available medical histories from FXTAS cases. We studied the association between changes in metabolite abundance in postmortem brain (ITG and CB) and the severity of core clinical symptoms (absent/mild vs. moderate/severe) per last medical report based on the treating physician’s perception during neurological evaluation. FXTAS stages (stages of physical disability defined by the consortium) were as follows: (1) subtle or questionable tremor and/or balance problems; (2) minor tremor and/or balance problems with minimal interference in activities of daily living (ADL); (3) moderate tremor and/or balance problems with significant interference in ADL; (4) severe tremor and/or balance problems with the need to use a cane or walker; (5) daily use of a wheelchair; and (6) bedridden [15]. Scores from standardized clinical or research assessments were also used when available (Supplementary Materials Table S3).

2.6. Statistical Methods

2.6.1. Differential Metabolite Abundance Analyses

Cases and controls were frequency matched by age and sex. Metabolomics data were log transformed and cyclic loess were normalized [16] prior to analysis. Differential metabolite abundance analyses were conducted using the Bioconductor package limma [17] version 3.52.2. Each brain region was analyzed separately. Models used in limma were adjusted for batch and subject age by including these as covariates (postmortem interval could not be controlled for as it was missing for several subjects but had a comparable distribution in cases and controls). Analyses were conducted using R version 4.2.1 (22 November 2022) [18]. Multiple comparisons were accounted for using the Benjamini–Hochberg false discovery rate (FDR adj. p-value). Metabolites were considered significant when the adj. p-value was calculated from the FDR < 0.05, and metabolites with adj. p-value < 0.08 were considered trending. Metabolites with raw p-values < 0.01 and adj. p-values < 0.2 were considered to have a possible association with changes in the number of CGG repeats. Volcano plots of metabolite log2FC and −log10 (p-value) values were also generated to identify metabolites of interest, with metabolites considered to be differentially expressed if they exhibited a p-value < 0.05.

2.6.2. Enrichment and Pathway Analyses

The differentially expressed metabolites from the comparative analyses of FXTAS versus control groups were subjected to enrichment analysis using the R package clusterProfiler [19], version 4.4.4, which implements the algorithm from GSEA [20]. This analysis is sensitive to identifying small but consistent changes among metabolites involved in the same biological pathway. Pathways were considered significant when the adj. p-value calculated from the enrichment analysis was <0.05 and trending when adj. p-value 0.05 < 0.08.

2.6.3. Receiver Operator Characteristic (ROC) Curve Analysis—Area under the Curve (AUC)

The ROC curve is the most popular graphical tool for evaluating the discriminatory power of a biomarker. To postulate potential predictive biomarkers of FXTAS, we tested the metabolites with a raw p-value < 0.05 in the differential metabolite abundance analysis and those metabolites found to be upregulated/downregulated in the volcano plot analysis for ITG and CB. A value of 0.5 for AUC suggested that the metabolite being tested had no discriminatory ability between FXTAS cases and controls. AUC values between 0.5–0.7 had poor discrimination, 0.7–0.8 had acceptable discrimination, 0.8–0.9 had excellent discrimination, and >0.9 had outstanding discrimination. The AUC values reported are in-sample values.

3. Results

A metabolomics dataset was generated from the frozen postmortem brain samples from FXTAS cases and age-/sex-matched controls. The untargeted analysis of the primary metabolites demonstrated significant alterations in the FXTAS brains. A complete list of the identified metabolites is available in the Supplementary Materials. A description of the key outcomes is presented below.

3.1. Participants

The age of the FXTAS cases ranged from 67 to 93 years (mean 80.8 years). The control cases ranged in age from 62 to 97 years (mean 80.5 years). The average CGG repeat number in the FXTAS group was 82 ± 15.7 (mean ± SD), and in the controls, it was 30.6 ± 4.8. A total of 100% of the cases (n = 44) were white. The age range for the onset of symptoms of FXTAS was 50–81 years (Table 1). Most of the FXTAS cases presented with ataxia as the first clinical manifestation, and 36% of the cases had additional neurological diagnoses (Supplementary Materials Table S3).

3.2. Primary Metabolite Profile in the Brains with FXTAS

The differential metabolite abundance analysis detected 513 primary metabolites, and 191 were identified (Supplementary Materials Tables S1 and S2). The changes in the metabolite relative abundance were region-specific. A total of 22 metabolites showed changes between groups (raw p-value < 0.05) in ITG. Cytidine abundance, a pyrimidine nucleoside, was lower in the FXTAS cases—log2 fold change (logFC): −1.38, adj. p-value 0.0007. In addition, 3-hydroxybutyric acid (logFC: 1.07, adj. p-value 0.0772), 2-hydroxybutanoic acid (logFC: 0.93, adj. p-value 0.0772), and 1,5-anhydroglucitol (logFC: 1.08, adj. p-value 0.0772), metabolites involved in energy metabolism, showed a trending higher abundance. CB showed changes in 21 metabolites (raw p-value < 0.05), and the relative abundance of oleamide, a fatty acid, was significantly lower (logFC: −1.06, adj. p-value 0.0018) (Table 2). Significance based on a volcano plot analysis showed the abundance of 3-hydrobutyric acid, 1,5-anhydroglucitol, and pyruvic acid to be upregulated; cytidine and glyceric acid were downregulated in the ITG of FXTAS cases compared to the controls. In CB, fructose-1-phosphate, 1,5-anhydroglucitol, 2-hydroxybutanoic acid, and glucose-6-phosphate were upregulated, while oleamide was observed to be downregulated, as shown in Figure 1a,b.

3.3. KEGG Enrichment Analysis

The enrichment of the KEGG pathway analysis in the ITG revealed that the most relevant downregulated pathways were related to the dysregulation of identified amino acids in FXTAS vs. controls: (i) protein digestion and absorption (hsa04974), (ii) aminoacyl-tRNA biosynthesis (hsa00970), and (iii) D-amino acid metabolism (hsa00470). Similar downregulation of (i) protein digestion and absorption (hsa04974) and (ii) D-amino acid metabolism (hsa00470) were noted in CB (Table 3). The enrichment analysis did not show any distinctive upregulated pathways.

3.4. Relationship between CGG Expansion and Metabolite Abundance

Forty-six metabolites had abundance changes associated with CGG repeat expansions (raw p-value < 0.05), eleven of which showed a possible correlation (raw p-value < 0.01 and adj. p-value < 0.2) between the relative abundance and the number of CGG repeats in ITG. Cytidine showed a significant decrease in abundance with smaller CGG expansions (55–74 repeats). Cases with larger expansions showed a larger logFC change; however, significance was not achieved after a multiple comparisons adjustment (logFC −1.59; adj. p-value 0.03 (55–74 vs. 20–44 repeats); logFC −1.26; adj. p-value 0.06 (75–94 vs. 20–44 repeats); logFC −1.82; adj. p-value 0.14 (75–94 vs. 95–120 repeats)) (Figure 2a,b). Five metabolites exhibited increases in abundance with increases in the CGG repeat number (which we refer to here as a “positive correlation”, although CGG was categorized in the analysis); 2-hydroxybutanoic acid showed a trending difference (adj. p-value 0.054) between cases with larger CGG expansions and controls (Supplementary Materials Figure S1), while adenosine, adipic acid, guanine, and pyrophosphate had possible positive correlations. Six metabolites showed a possible negative correlation: histidine, cytidine, asparagine, xanthine, proline, and heptadecanoic acid. In the CB, 42 metabolites had abundance changes; oleamide and fructose-1-phosphate presented a possible correlation (Supplementary Materials Figure S1). Further analysis of the cases showed a significant decrease in the abundance of UDP-glucuronic acid (logFC: −1.66, adj. p-value 0.04)—a sugar made from UDP-glucose using NAD+ as cofactor and used in the synthesis of polysaccharides and ascorbic acid—in cases with larger expansions (>95 CGG repeats) when compared to smaller expansions in the premutation range (Figure 2g). Conflicting results were found in the plasma samples from FMR1 premutation carriers, with a positive correlation between the relative abundance of proline and UDP-glucuronic acid, and in those with larger CGG expansions [21]. Oleamide, on the other hand, had a negative correlation with the number of CGG repeats in the plasma from premutation carriers with and without FXTAS, which was in agreement with our results in the postmortem brain samples (Figure 2f). There is a possibility that the lack of significance can be attributed to the limited number of FXTAS cases included in the small expansion (CGG 55–74, n = 7) and large expansion (CGG > 95, n = 4) groups during the analysis.

3.5. ROC Curve Analysis

Based on our results from the differential metabolite abundance analyis and volcano plot analysis, we tested 22 metabolites in the ITG and 21 in the CB (see Table 2) with raw p-values < 0.05 for their discriminatory potential between the FXTAS cases and controls. We found cytidine to have outstanding discriminatory potential in the ITG, followed by 3-hydroxybutyric acid and xanthine with excellent discriminatory potential and 1,5-anhydroglucitol, oleamide, 2-hydroxybutanoic acid, and guanine with acceptable discriminatory potential. In the CB, oleamide and lysine had excellent discriminatory potential, while fructose-1-phosphate, N-acetylmannosamine 1,5-anhydroglucitol, and 2-hydroxybutanoic acid had acceptable discriminatory potential. The remaining tested metabolites had no/poor discriminatory potential for FXTAS vs. controls. (See Figure 3).

3.6. Association between Changes in Metabolite Abundance and Markers of Neurodegeneration

We identified possible associations between markers of neurodegeneration (presence/absence) in the ITG of FXTAS cases and changes in metabolite abundance. Uracil was increased by 1.30 logFC (raw p-value 0.0007, adj. p-value 0.121) in the cases with neurofibrillary tangles (81%, n = 18), and 2,5-dihydroxypyrazine was significantly decreased by −1.61 logFC (adj. p-value 0.047) (Figure 2c,d) in the cases with microhemorrhages (50%, n = 11). Our group previously reported that about 50–60% of FXTAS cases present with microhemorrhages in the white matter of the cerebral and cerebellar cortices [14].

3.7. Association between Changes in Metabolite Abundance and Clinical Symptoms

The abundance of xanthosine, a purine nucleoside, was increased by 2.2 logFC with trending significance (adj. p-value 0.061) in the CB of the FXTAS cases with a clinical diagnosis of dementia vs. those with no/mild cognitive decline per medical history (Figure 2h). No other association was found between the studied symptoms of FXTAS and the changes in metabolite abundance in the ITG and CB.

4. Discussion

Our analysis generated untargeted metabolomics data in postmortem brains with FXTAS. The metabolomics analysis identified disease-related metabolic changes. These data are the starting point to map the metabolic trajectory leading to disease onset and progression. We identified a set of altered metabolites, enhanced pathways, and potential biomarkers of disease at the later stages of FXTAS progression. All the cases evaluated had an advanced clinical involvement. In this section, we discuss our findings, suggesting remarkable metabolic abnormalities in the pathways involved in cellular membrane synthesis, response to oxidative stress, and energy utilization.

4.1. Pathways Involved in Neuronal Membrane Synthesis Are Affected in FXTAS

Cytidine’s relative abundance within the ITG was lower (logFC −1.38, adj. p-value 0.0007) and showed an optimal discriminatory power between the FXTAS cases and controls (Figure 3a). Its abundance decreased remarkably in the FXTAS cases with larger CGG expansions when compared to the controls with normal (20–44) CGG repeat numbers (Figure 2b). Cytidine is a pyrimidine nucleoside comprising a cytosine nucleic base and ribose sugar and is acquired through dietary sources. Cytidine is a substrate in the production of critical membrane phospholipids, phosphatidylcholine, and phosphatidylethanolamine and is also a precursor in pyrimidine nucleotide synthesis concerning uracil and cytidine triphosphate (CTP). A prerequisite for the brain’s utilization of cytidine is its conveyance from the circulation into the brain’s extracellular fluid and its incorporation into the neurons and glia. The effectual mechanism facilitating the brain’s uptake of cytidine has yet to be identified [22].
Given the diminished relative abundance of cytidine in the brains affected by FXTAS, it is reasonable to anticipate dysregulations in the Kennedy and pyrimidine salvage pathways (Figure 4a). These pathways are involved in polysaccharide and phospholipid biosynthesis, detoxification processes, and protein and lipid glycosylation [23]. The disruption of new neuronal membrane synthesis could be linked to the etiology of neurodegeneration [24]. Interestingly, higher cytidine levels were observed in the biofluids of a small cohort of patients with various neurodegenerative dementias, including Alzheimer’s, frontotemporal dementia, and Lewy body dementia [25], and they have been postulated as a plasma marker for Alzheimer’s disease [26]. However, each form similarly involves the degradation of vulnerable neurons of various brain regions and shares a related symptomology to FXTAS, such as cognitive decline and impairments of body movement [27,28,29]. Changes in cytidine abundance were not reported in the plasma from FXTAS patients; however, a decreased level of choline phosphate, an intermediate of the Kennedy pathway, was observed in FMR1 premutation carriers who had developed FXTAS [5]; a lower abundance of cytidine was also found in the cerebellum of premutation mice [6]. A previous open-label pilot clinical trial of citicoline, an intermediate in the Kennedy pathway, did not show clinical efficacy (expected as a 20% improvement on the FXTAS rating scale); however, after one year of treatment, none of the participants had significantly worse scores from the baseline [30].
Additional studies are necessary to understand the FXTAS-related biological abnormalities involved in cellular membrane synthesis, including those involved in the synthesis of phospholipids, which are essential for membrane fusion, mitochondrial stability, protein biosynthesis, and oxidative phosphorylation [31]. Further analysis of lipids in brains with FXTAS is needed to fully evaluate the impact of having a lower abundance of cytidine in the critical pathways of phospholipid biosynthesis and lipid glycosylation.

4.2. Brains with FXTAS Present with a High Abundance of Markers for Oxidative Stress

A higher relative abundance of 2-hydroxybutanoic acid (2HB) was observed within the ITG and CB in the FXTAS cases when compared to the controls. The abundance increased in the ITG of the FXTAS cases with increasing CGG repeat expansions (Supplemental Materials Figure S1). 2HB is formed from its derivative alpha-ketobutyrate (AKB), a by-product of threonine and methionine catabolism and glutathione (GSH) anabolism [32,33]. GSH is the primary intracellular antioxidant, and its production is increased during increased oxidative stress [34]. Under normal conditions, AKB is converted to propionyl-CoA, a key player in the citric acid cycle (TCA). When AKB production outpaces its catabolism, 2HB is formed. A high NADH/NAD+ ratio potentiates this process by increasing the activity of lactate dehydrogenase, an enzyme that catalyzes the conversion of AKB to 2HB [33] (Figure 4c).
In addition, an increased 2HB level is a well-established biomarker for insulin resistance (IR), impaired glucose tolerance, metabolic acidosis, and oxidative stress [33,34,35,36]. The aforementioned NADH/NAD+ imbalance may be attributed to increased lipid oxidation, a common feature of IR and a source of oxidative stress. Increased 2HB concentrations have also been reported in biofluids in schizophrenia patients [37], inherited metabolic diseases [38], and multiple sclerosis [39], and it showed discriminatory power between the FXTAS cases and controls in our analysis (Figure 3f,m). Interestingly, Giulivi et al. reported a lower 2HB plasma abundance in premutation carriers compared to controls [4]. However, a second study reported an increased abundance of 2HB in plasma collected from a larger cohort of FMR1 premutation carriers compared to healthy controls. The same study did not find a difference in the relative abundance of 2HB between premutation carriers that had developed FXTAS and those that had not during the study period [5] (Table 4). To date, no study has correlated abnormal 2HB concentrations to specific FXTAS symptoms or other neurodegenerative conditions.
The relative abundance of proline was decreased in the ITG and CB of the FXTAS cases. Contrarily, the abundance of proline was increased in the plasma of FMR1 premutation carriers (Table 4) [4,21]. Prior studies on the regulatory roles of proline biosynthesis suggested the role of the proline cycle in augmenting redox cycling for the Warburg effect, increasing the level of total pyridine nucleotides and increasing the capacity for antioxidant defenses by stimulating the oxidative branch of the pentose phosphate pathway [40].
Further, the relative abundance of the nucleoside xanthosine was increased (adj. p-value 0.061) in the CB of the FXTAS cases with a clinical diagnosis of dementia. Purine metabolism is a component of the mitochondrial response to oxidative stress and may be involved in slowing progressive mitochondrial dysfunction. High levels of xanthosine have been reported in the plasma of patients with untreated schizophrenia [41]. Changes in xanthosine and guanine expression were also detected in postmortem entorhinal cortex in AD [42].

4.3. Dysfunction in Energy Metabolism in Brains with FXTAS

Our analysis found a trending (adj. p-value 0.077) increased relative abundance of 3-hydroxybutiric acid, a fatty acid and marker for impaired glucose regulation, and 1,5-anhydroglucitol, a glucose analog in ITG, as well as a significantly (adj. p-value 0.0018) decreased relative abundance of oleamide in the CB. The volcano plots additionally identified fructose-1-phosphate and glucose-6-phosphate to be differentially expressed in the CB, while intermediate metabolites involved in glycolysis, including glyceric acid and pyruvic acid, were differentially expressed in the ITG (Figure 1a,b), demonstrating a dysfunction in glucose utilization (Figure 4c). These results provide additional evidence supporting the presence of altered energy metabolism in the brains of FXTAS individuals. Fatty acids are among the most important lipid molecules that determine the brain’s integrity and function [43]. Changes in the plasma metabolic profile of fatty acids have been reported in patients diagnosed with FXTAS [5] and in various neurodegenerative and neuropsychiatric disorders such as Alzheimer’s disease, autism spectrum disorders, schizophrenia, anxiety, and FXTAS [44,45,46].

4.4. A Central-Acting Fatty Acid Shows Lower Abundance in FXTAS

The relative abundance of oleamide was significantly lower (logFC: −1.06, adj. p-value 0.0018) in the CB with FXTAS (Figure 2e). In addition, oleamide showed an excellent discriminatory power during the ROC analysis (Figure 3h). Oleamide is a fatty acid primary amide, which exerts effects on vascular and neuronal tissues. The recognized biological effects of oleamide include the binding of cannabinoid receptors [47], specifically CB1 [48,49], immunological suppression, sleep induction, serotonin and gamma-aminobutyric acid receptor activation [50,51], and the inhibition of gap junction [52] coupling (Figure 4b). In addition, in a mouse model of AD, exogenous oleamide supplementation reduced amyloid-β (Aβ) accumulation and suppressed inflammation after amyloid Aβ deposition by enhancing microglial phagocytosis [53]. Furthermore, oleamide may elicit protective effects against excitotoxicity-induced neuronal death, partly related to its direct and/or indirect calpain inhibitory activity [54] and other yet unknown biological actions.
Gap junctions are found between neurons and glial cells [52]. Gap junctions play an important role in neuronal activity and are implicated in many cognitive processes, including the modulation of memory processes, brain development, and the mechanisms of psychostimulant dependence [55]. In addition, glial gap junctions modulate the expression of hundreds of genes [56] and enable the propagation of signaling molecules implicated in cerebrovascular regulation [57] and astrocytic glutamate release [58,59,60,61]. A low relative abundance of oleamide in brains with FXTAS may therefore affect neuronal excitability and facilitate a higher permeability by microvascular endothelial cells. A lack of the beneficial effects of oleamide in brains with FXTAS may also contribute to lowering the brain’s capacity to respond to the damage caused by inflammation and excitotoxicity, as well as promoting a higher accumulation of Aβ in brains with FXTAS and concomitant AD. Decreased oleamide plasma levels were reported in FMR1 premutation carriers and proposed as a potential biomarker for the diagnosis of FXTAS [21], showing a negative correlation with CGG expansion [4], which was also identified in our analysis. A lower abundance of oleamide in plasma has also been postulated as a marker for AD [62].

4.5. Study Advantages

Submitting a variety of tissues to metabolomic studies is important as each tissue has its own unique advantages. For example, CSF is less prone to metabolite degradation in comparison to plasma because of its relatively decreased enzymatic activity [63]. Plasma, however, is easier to obtain in larger quantities. In the study of neurogenerative disease, metabolomic analysis of brain tissue offers the advantage of the direct quantification of metabolites at the site of the organ of interest. This helps limit the confounding variables introduced by the analysis of tissues separate from the brain. This is especially relevant in the case of brains where the blood–brain barrier (BBB) and the blood–CSF barrier (BSFB) reduce the influx and efflux of small molecules such as metabolites [64].
Using a combination of tissues helps to strengthen the search for disease biomarkers. Metabolites found in multiple tissues to be abnormally altered compared to controls raises confidence in results being related to a disease rather than an artifact of pretreatment, sampling, or some other unknown variable impacting a single tissue type. In addition, finding biomarkers of disease to be universal across a tissue will allow for easier disease staging via the testing of more readily acquired tissue, such as plasma. We compared our results to previously reported metabolomics analyses conducted in plasma from FMR1 premutation with and without FXTAS patients and frozen brain tissue from a mouse model of FXTAS (Table 4).

4.6. Study Limitations

Differences in the postmortem interval (PMI) pose a significant limitation. Certain changes in metabolite concentrations might not necessarily indicate disease pathology, but rather a difference between antemortem and postmortem conditions. Several studies have found metabolites altered by PMI in a variety of tissues [65,66,67]. Studies in sheep and human brains found free trimethylammonium (fTMA), propionate, and butyrate to be elevated as PMI increased [68,69]. Our analysis was limited to primary metabolites using GC-MS quantification. Further analysis of lipid metabolomics is granted to validate our findings and for a more comprehensive comparison between lipid metabolic disturbances found in the plasma of FMR1 premutation carriers and FXTAS patients.

5. Conclusions

An altered abundance of some metabolites may influence the clinical outcome of patients with FXTAS. Our data identified alterations in the metabolic pathways related to oxidative stress responses and bioenergetics in the human postmortem brains with FXTAS. The results from our study demonstrate similarities with previous metabolic disturbances reported in plasma of FMR1 premutation carriers and FXTAS patients, but they also demonstrate differences that need further analysis. The significantly lower abundance of cytidine and oleamide in the FXTAS brains and their discriminatory potential between diseased and healthy brain tissue should serve as starting point to study their potential protective effects against neuronal damage in FXTAS. Oleamide and other metabolites of interest previously reported in plasma and now reported in postmortem brains suggest their potential as biomarkers for the diagnosis and prognosis of FXTAS.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/cells12172132/s1, Table S1: List of identified metabolites in ITG; Table S2: List of identified metabolites in CB; Table S3: Clinical and pathological measures; Figure S1: Correlation of metabolite abundance with CGG expansion.

Author Contributions

Conceptualization, M.J.S.-A. and V.M.-C.; methodology, M.J.S.-A. and V.M.-C.; formal analysis, M.J.S.-A., B.D.-J. and V.M.-C.; investigation, M.J.S.-A., M.D.J., Y.A.M., E.L.M., A.P.P., P.J. and Y.H.H.; data curation, West Coast Metabolomics Center; writing—original draft preparation, M.J.S.-A., M.D.J., Y.A.M., E.L.M., A.P.P., P.J. and Y.H.H.; writing—review and editing, B.D.-J., F.T., R.J.H. and V.M.-C.; visualization, M.J.S.-A. and M.D.J.; supervision, V.M.-C.; project administration, M.J.S.-A. and V.M.-C.; funding acquisition, V.M.-C. and R.J.H. All authors have read and agreed to the published version of the manuscript.

Funding

This research was supported by funds from the National Institute of Neurological Disorders and Stroke (NINDS), grant R01 1NS107131, the National Institute of Child Health and Human Development (NICHD), grant R01 HD036071, the MIND Institute Intellectual and Developmental Disabilities Research Center, P50 HD103526, and the Shriners Hospitals for Children, Northern California.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

All donors gave written informed consent for a brain autopsy and the use of the material and clinical information for research purposes.

Data Availability Statement

All metabolomics data generated, in both raw and processed format, are available at the Metabolomics Workbench www.metabolomicsworkbench.org (accessed on 22 August 2023).

Acknowledgments

The authors acknowledge the unconditional support to FMR1-related conditions research from all donors and their families.

Conflicts of Interest

R.J.H. has received funding from Zynerba, Ovid, and the Azrieli Foundation for carrying out treatment studies in patients with fragile X syndrome (FXS). She has also consulted with Fulcrum, Ovid, and Zynerba regarding treatment studies in individuals with FXS. F.T. has received funds from Asuragen and Zynerba for studies in FXS and associated disorders. V.M.-C. has consulted with Paxmedica and received funding from Zynerba for the organization of conferences in FXS and associated disorders. The authors have no conflict of interest to declare that are relevant to the content of this article. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

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Cells 12 02132 g001
Figure 1. (a,b) Volcano plots for differential metabolite abundance analysis of case versus control in ITG and CB. The x-axis shows the logFC for case/control, and the y-axis shows −log10 of the false discovery rate adjusted p-value. Metabolites with an absolute logFC greater than 1 are shown in pink and labeled with their BinBase name. The dashed horizontal line shows an adjusted p-value of 0.05. The dashed vertical lines show log2 fold changes of −1 and 1. A metabolite was said to be differential if p-value < 0.05 and logFC > 1. The purple dots in the figure represent downregulated differentially expressed metabolites, the red dots represent upregulated differentially expressed metabolites, and the gray dots represent metabolites detected but that were not significantly different. (c–e) Heatmaps of altered metabolites. Heatmaps were created using the Bioconductor package ComplexHeatmap, version 2.12.0. Red indicates high and purple indicates low abundance of the metabolite relative to the median.
Figure 1. (a,b) Volcano plots for differential metabolite abundance analysis of case versus control in ITG and CB. The x-axis shows the logFC for case/control, and the y-axis shows −log10 of the false discovery rate adjusted p-value. Metabolites with an absolute logFC greater than 1 are shown in pink and labeled with their BinBase name. The dashed horizontal line shows an adjusted p-value of 0.05. The dashed vertical lines show log2 fold changes of −1 and 1. A metabolite was said to be differential if p-value < 0.05 and logFC > 1. The purple dots in the figure represent downregulated differentially expressed metabolites, the red dots represent upregulated differentially expressed metabolites, and the gray dots represent metabolites detected but that were not significantly different. (c–e) Heatmaps of altered metabolites. Heatmaps were created using the Bioconductor package ComplexHeatmap, version 2.12.0. Red indicates high and purple indicates low abundance of the metabolite relative to the median.
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Figure 2. (a) Cytidine’s differential abundance cases/controls. (b) Cytidine abundance was significantly lower in the 55–74 CGG range group compared to controls in the ITG. (c) Uracil was increased, with trending significance, in the ITG of subjects with identified neurofibrillary tangles. (d) 2,5-dihydroxypyrazine was significantly decreased in cases with microhemorrhages in the ITG. In cerebellum: (e) Oleamide abundance was lower in FXTAS cases compared to controls. (f) Differences in relative abundance (raw p < 0.001) were found in the 55–74 and 75–94 CGG range groups. Significance was not achieved after multiple testing adjustment. (g) Significantly lower abundance of UDP-glucuronic acid in cases with larger expansions > 95 CGG repeats when compared to cases with 55–94 CGG repeats. (h) Xanthosine was increased, with trending significance, in the CB of cases with dementia diagnosis per medical history. * adj. p-value < 0.05, ** adj. p-value < 0.01, *** adj. p-value < 0.001, † raw p-value < 0.0015.
Figure 2. (a) Cytidine’s differential abundance cases/controls. (b) Cytidine abundance was significantly lower in the 55–74 CGG range group compared to controls in the ITG. (c) Uracil was increased, with trending significance, in the ITG of subjects with identified neurofibrillary tangles. (d) 2,5-dihydroxypyrazine was significantly decreased in cases with microhemorrhages in the ITG. In cerebellum: (e) Oleamide abundance was lower in FXTAS cases compared to controls. (f) Differences in relative abundance (raw p < 0.001) were found in the 55–74 and 75–94 CGG range groups. Significance was not achieved after multiple testing adjustment. (g) Significantly lower abundance of UDP-glucuronic acid in cases with larger expansions > 95 CGG repeats when compared to cases with 55–94 CGG repeats. (h) Xanthosine was increased, with trending significance, in the CB of cases with dementia diagnosis per medical history. * adj. p-value < 0.05, ** adj. p-value < 0.01, *** adj. p-value < 0.001, † raw p-value < 0.0015.
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Figure 3. ROC analysis. (a–g) Metabolites with discriminatory potential between FXTAS and controls in the ITG. (h–m) Metabolites with discriminatory potential between FXTAS and controls in the CB. AUC less than or equal to 0.5 = no discriminatory potential, AUC 0.5–0.7 = poor discriminatory potential, AUC 0.7–0.8 = acceptable discriminatory potential, AUC 0.8–0.9 = excellent discriminatory potential, and AUC > 0.9 = outstanding discriminatory potential. AUC values reported are in-sample values.
Figure 3. ROC analysis. (a–g) Metabolites with discriminatory potential between FXTAS and controls in the ITG. (h–m) Metabolites with discriminatory potential between FXTAS and controls in the CB. AUC less than or equal to 0.5 = no discriminatory potential, AUC 0.5–0.7 = poor discriminatory potential, AUC 0.7–0.8 = acceptable discriminatory potential, AUC 0.8–0.9 = excellent discriminatory potential, and AUC > 0.9 = outstanding discriminatory potential. AUC values reported are in-sample values.
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Figure 4. (a) Kennedy and pyrimidine salvage pathways, adapted from Cansev 2006 [22]. (b) Diagram showing interactions of oleamide in CNS. (c) Several metabolites involved in energy homeostasis and stress response pathways were identified to have changes in relative abundance in FXTAS. Red arrows show changes in the relative abundance of metabolites in the FXTAS group compared to controls. † Metabolites with discriminatory power between the FXTAS cases and controls.
Figure 4. (a) Kennedy and pyrimidine salvage pathways, adapted from Cansev 2006 [22]. (b) Diagram showing interactions of oleamide in CNS. (c) Several metabolites involved in energy homeostasis and stress response pathways were identified to have changes in relative abundance in FXTAS. Red arrows show changes in the relative abundance of metabolites in the FXTAS group compared to controls. † Metabolites with discriminatory power between the FXTAS cases and controls.
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Table 1. Subject characteristics.
Table 1. Subject characteristics.
Control (n = 19)Case (n = 25)p-Value
Age 0.868
N1925
Mean (SD)80.5 (8.5)80.8 (7.2)
Median (Range)80 (62–97)81 (67–93)
PMI 0.939
N1918
Mean (SD)23.5 (33)21.5 (28.7)
Median (Range)8.7 (2.3–136.1)15.2 (2.5–127)
Gender 1
F9 (47.4%)12 (48%)
M10 (52.6%)13 (52%)
Blood CGG
N1925
Mean (SD)30.6 (4.8)82 (15.7)
Median (Range)30 (23–42)81 (55–120)
20–4419 (100%)0
45–5400
55–7407 (28%)
75–94014 (56%)
95–12004 (16%)
Race/Ethnicity
White19 (100%)25 (100%)
Table 2. Differential abundance cases/controls.
Table 2. Differential abundance cases/controls.
Inferior Temporal GyrusCerebellum
BinBase NameBiological RolePubChemlogFCp-Valueadj. p-ValueBinBase NameBiological RolePubChemlogFCp-Valueadj. p-Value
Cytidinenucleoside6175−1.3810.0000040.0007Oleamidefatty acid amide5283387−1.0600.000010.0018
3-hydroxybutyric acidketone body921351.0750.00110.0772Fructose-1-phosphatemonosaccharide4393941.3620.00130.1067
2-hydroxybutanoic acidorganic acid4408640.9390.00150.07721,5-anhydroglucitolmonosaccharide649601.0370.00390.1683
1,5-anhydroglucitolmonosaccharide649601.0830.00160.0772Phosphoethanolaminephosphomonoester10150.5690.00460.1683
Oleamidefatty acid amide5283387−0.9040.00290.11152-hydroxybutanoic acidorganic acid4408641.0560.0050.1683
Guanineamino acid/purine7640.7470.00430.1181N-acetylmannosamineamino sugar11096158−0.7790.00690.1796
Xanthineamino acid/purine1188−0.3470.00430.1181Lysineessential amino acid5962−0.3980.00740.1796
Histidineessential amino acid6274-0.6240.00570.138Maltotriosetrisaccharide4395860.9080.01030.2134
Prolineessential amino acid145742−0.7300.00940.1854Prolineessential amino acid145742−0.9090.01140.2134
Phosphoric acidorganic acid10040.4870.00960.1854Threonineessential amino acid6288−0.3230.01410.2353
Gluconic acidorganic acid6857417−0.9520.01180.207Maltosedisaccharide4391860.9550.01610.2353
Cysteineamino acid5862−0.8140.01390.2232Xylosemonosaccharide1351910.6580.01670.2353
Cholesterollipid59970.3540.01680.2493Erythritolmonosaccharide polyol2222850.4340.02340.2906
Threonineessential amino acid6288−0.2650.01810.2493Glycyl tyrosinedipeptide92829−0.5910.02410.2906
Maltotriosetrisaccharide4395860.7520.01990.2557Inosinenucleoside60210.7800.02680.3021
Glyceric acidorganic acid752−1.7100.02190.2592Ascorbic acidorganic acid546700670.4800.02940.3107
Pyrophosphoric acidinorganic acid10230.4460.02280.2592Glycerolsugar alcohol753−0.3490.03190.315
Pyruvic acidorganic acid10601.0190.030.3145Histidineessential amino acid6274−0.6020.03360.315
Erythrosemonosaccharide439574−0.4610.0310.3145Arabitolsugar alcohol941540.3640.03560.3162
Adipic acidorganic acid1960.2830.04050.3904Serinenon-essential amino acid5951−0.3420.04130.3466
Gluconic acid lactoneorganic acid7027−0.6530.04510.4145Leucineessential amino acid6106−0.3290.04860.3466
Adenosinenucleoside609610.6390.04740.4161
Table 3. Enriched pathways in FXTAS vs. controls.
Table 3. Enriched pathways in FXTAS vs. controls.
ITG
Pathway NameBinBase NamelogFCp-Valueadj. p-ValuetKEGG
Histidine−0.6240.0060.138−2.912C00135
Protein digestion and absorption
Homo sapiens (human)
hsa04974
p-value: 0.0019 adj. p-value 0.009
q-value 0.0039
Normalized enrichment score: −1.948		Proline−0.7300.0090.185−2.723C00148
Cysteine−0.8140.0140.223−2.568C00097
Threonine−0.2650.0180.249−2.460C00188
Methionine−0.2840.0910.446−1.728C00073
Leucine−0.1940.2130.585−1.265C00123
Glutamine0.1870.4420.7370.776C00064
Aspartic acid−0.0850.6080.803−0.516C00049
Alanine−0.0660.7080.870−0.377C00041
Aryptophan−0.0380.7990.891−0.256C00078
Aminoacyl tRNA biosynthesis
Homo sapiens (human)
hsa00970
p-value: 0.0053 adj. p-value 0.0132
q-value 0.0056
Normalized enrichment score: −1.868		Histidine−0.6240.0060.138−2.912C00135
Threonine−0.2650.0180.249−2.460C00188
Isoleucine−0.2760.0590.446−1.938C00407
Glycine−0.2360.0650.446−1.896C00037
Serine−0.2520.0800.446−1.816C00065
Asparagine−0.1390.2730.643−1.110C00152
O-phosphoserine0.6270.2950.6431.148C01005
Tyrosine−0.1020.5470.801−0.608C00082
Lysine−0.0740.6220.811−0.497C00047
Tryptophan−0.0380.7990.891−0.256C00078
D-amino acid metabolism
Homo sapiens (human)
hsa00470
p-value: 0.0391 adj. p-value 0.065
q-value 0.0274
Normalized enrichment score: −1.641		Proline−0.7300.0090.185−2.723C00148
Cysteine−0.8140.0140.223−2.568C00097
Threonine−0.2650.0180.249−2.460C00188
Methionine−0.2840.0910.446−1.728C00073
Glutamic acid−0.1230.2620.629−1.137C00025
N-acetylglutamate−0.1710.3210.671−1.006C00624
Trans-4-hydroxyproline0.1390.3500.6750.946C01157
Glutamine0.1870.4420.7370.776C00064
Aspartic acid−0.0850.6080.804−0.516C00049
Alanine−0.0660.7080.870−0.377C00041
Phenylalanine−0.0070.9640.969−0.045C00079
CB
Pathway NamePathway NamelogFCp-ValueAdjusted p-ValuetKEGG
Protein digestion and absorption
Homo sapiens (human)
hsa04974
p-value: 0.0004 adj. p-value 0.002
q-value 0.0009
Normalized enrichment score: −2.017		Proline−0.9090.0110.213−2.649C00148
Threonine−0.3220.0140.235−2.562C00188
Histidine−0.6020.0340.315−2.198C00135
Leucine−0.3290.0490.347−2.031C00123
Cysteine−0.6180.1150.448−1.609C00097
Methionine−0.3130.1240.448−1.570C00073
Alanine−0.3300.1370.448−1.518C00041
Aspartic acid−0.2720.1550.469−1.447C00049
Glutamine0.1480.5310.8060.632C00064
Tryptophan−0.0890.6080.853−0.517C00078
D-amino acid metabolism
Homo sapiens (human)
hsa00470
p-value: 0.0034 adj. p-value 0.0069
q-value 0.0036
Normalized enrichment score: −1.815		Proline−0.9090.0110.18−2.814C00148
Threonine−0.3220.0140.235−2.562C00188
Cysteine−0.6180.1150.315−2.198C00097
Methionine−0.3130.1240.347−2.112C00073
Alanine−0.330.1370.347−2.018C00041
Aspartic acid−0.2720.1550.473−1.427C00049
Phenylalanine−0.1260.4200.731−0.815C00079
Glutamine0.1480.5310.8060.632C00064
Trans-4-hydroxyproline−0.0730.7020.895−0.386C00157
N-acetylglutamate−0.0220.9020.982−0.124C00624
Glutamic acid−0.0080.9540.982−0.058C00025
Table 4. Metabolomics in FMR1 premutation.
Table 4. Metabolomics in FMR1 premutation.
ITG
BinBase NameFMR1 FXTAS
Human
Frozen Brain		Zafarulla 2020 [5]
FMR1 FXTAS
Human Plasma		Giulivi 2016 [4,21]
FMR1 Premutation
Human Plasma
3-hydroxybutyric acid↑↑↓
2-hydroxybutanoic acid↑↑↓
Oleamide↓ ↓
Histidine↓ ↓
Proline↓ ↑
Gluconic acid↓ ↑
Glyceric acid↓ ↑
Pyruvic acid↑↑
CB
BinBase nameFMR1 FXTAS
Human
Frozen brain		Zafarulla 2020 [5]
FMR1 FXTAS
Human Plasma		Giulivi 2016 [4,21]
FMR1 premutation
Human Plasma		Kong [6]
FMR1 mouse
Frozen CB
Oleamide↓ ↓
1,5-anhydroglucitol↑ No change
Phosphoethanolamine↑↑ No change
2-hydroxybutanoic acid↑↑↓
Lysine↓ ↓↑
Proline↓ ↑No change
Threonine↓ No change
Maltose↑ ↑
Xylose↑ ↑
Erythritol↑ No change
Inosine↑ ↑
Glycerol↓↑↑↓
Histidine↓ ↓No change
Serine↓ No change
Leucine↓ ↑
↑ Increased, ↓ decreased
Similar results between FXTAS frozen brain and plasma from premutation carriers with FXTAS
Similar results between FXTAS frozen brain and plasma from premutation carriers without FXTAS
Similar results between frozen cerebellum in FXTAS cases and an FMR1 mouse model
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Salcedo-Arellano, M.J.; Johnson, M.D.; McLennan, Y.A.; Hwang, Y.H.; Juarez, P.; McBride, E.L.; Pantoja, A.P.; Durbin-Johnson, B.; Tassone, F.; Hagerman, R.J.; et al. Brain Metabolomics in Fragile X-Associated Tremor/Ataxia Syndrome (FXTAS). Cells 2023, 12, 2132. https://doi.org/10.3390/cells12172132

AMA Style

Salcedo-Arellano MJ, Johnson MD, McLennan YA, Hwang YH, Juarez P, McBride EL, Pantoja AP, Durbin-Johnson B, Tassone F, Hagerman RJ, et al. Brain Metabolomics in Fragile X-Associated Tremor/Ataxia Syndrome (FXTAS). Cells. 2023; 12(17):2132. https://doi.org/10.3390/cells12172132

Chicago/Turabian Style

Salcedo-Arellano, Maria Jimena, Michael D. Johnson, Yingratana A. McLennan, Ye Hyun Hwang, Pablo Juarez, Erin Lucille McBride, Adriana P. Pantoja, Blythe Durbin-Johnson, Flora Tassone, Randi J. Hagerman, and et al. 2023. "Brain Metabolomics in Fragile X-Associated Tremor/Ataxia Syndrome (FXTAS)" Cells 12, no. 17: 2132. https://doi.org/10.3390/cells12172132

APA Style

Salcedo-Arellano, M. J., Johnson, M. D., McLennan, Y. A., Hwang, Y. H., Juarez, P., McBride, E. L., Pantoja, A. P., Durbin-Johnson, B., Tassone, F., Hagerman, R. J., & Martínez-Cerdeño, V. (2023). Brain Metabolomics in Fragile X-Associated Tremor/Ataxia Syndrome (FXTAS). Cells, 12(17), 2132. https://doi.org/10.3390/cells12172132

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