Next Article in Journal
ProNGF/p75NTR Axis Drives Fiber Type Specification by Inducing the Fast-Glycolytic Phenotype in Mouse Skeletal Muscle Cells
Previous Article in Journal
Mechanistic Actions of microRNAs in Diabetic Wound Healing
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Cells
Volume 9
Issue 10
10.3390/cells9102230
Review Report
cells-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
Article
Peer-Review Record

TAZ Represses the Neuronal Commitment of Neural Stem Cells

Cells 2020, 9(10), 2230; https://doi.org/10.3390/cells9102230
by Natalia Robledinos-Antón 1,2,3,4,†, Maribel Escoll 1,2,3,4,†, Kun-Liang Guan 5 and Antonio Cuadrado 1,2,3,4,*
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Cells 2020, 9(10), 2230; https://doi.org/10.3390/cells9102230
Submission received: 15 July 2020 / Revised: 26 September 2020 / Accepted: 29 September 2020 / Published: 2 October 2020
(This article belongs to the Section Stem Cells)

Round 1

Reviewer 1 Report

I commend the authors for their good work on the transcriptional co-factor TAZ and its influence on Neural Stem Progenitor Cells (NSPC) of the mammalian brain.

Their identification of TAZ and its expression in NSPC declines with aging will contribute towards reputable knowledge in the field.

However, to further characterize some of the mechanisms involved, it would have been important to see experimental work conducted directly on the cell cycle. The authors do not demonstrate this approach. Accordingly, it will be of higher impact if they can demonstrate this strategy? Respectively.

Can the authors conduct such a strategy before the manuscript is accepted?

Author Response

Please see attached document with answer.

Author Response File: Author Response.pdf

Reviewer 2 Report

In the present study, authors investigated the role of TAZ on neuronal differentiation in vivo and in vitro. Authors showed that TAZ knock-down and forced expression in NSPCs promoted and inhibited neuronal differentiation, respectively. In addition, authors showed that forced expression of TAZ in NSPCs displayed down-regulated expression of Sox2 and proneuronal genes, such as ASCL1, NEUROG2, and NEUROD1. In general, this manuscript is well organized and written. I have a few comments as flowing:

 

  1. Fig. 1 and Fig. 2: Although some nestin+ NSPCs expressed TAZ during the development, it seems that there are many TAZ+ cells that lacked nestin expression. Do you know which types of cells express TAZ other than NSPCs during the development?

 

  1. Fig. 4A and Fig. 7A: It is better to add the data of nestin and DCX expression.

 

  1. Sox2 is an essential factor for directing various types of cells to neuronal lineage (Cell Stem Cell. 2012;11:471-476; Cell Stem Cell. 2012;11:100-109; Cell Stem Cell. 2011;8:538-551) as well as acquiring stemness by somatic cells (Cell. 2007;131:861-872). A previous study showed that forced expression of Sox2 promoted neurogenesis as well as up-regulated expressions of neurogenic transcription factors, such as MASH1 (ASCL1) and NEUROG2 (Stem Cells 2015, 33, 1962-74), indicating that expression patterns of Sox2 and these proneuronal genes may be associated under certain conditions. Please discuss this.

Author Response

Please see attached document with answer

Author Response File: Author Response.pdf

Round 2

Reviewer 2 Report

I think authors significantly improved their original manuscript based on reviewers’ comments.

Back to TopTop