Chia (Salvia hispanica L.) Seed Soaking, Germination, and Fatty Acid Behavior at Different Temperatures
, Jorge E. Campos 2, Alma Orozco-Segovia 3 and Cesar M. Flores-Ortiz 1,4,*Round 1
Reviewer 1 Report
Minor typos:
row 141: this expression is not clear ”Seed samples in a range of 200 300 mg”
r. 210: wrong ”At 10 °C , maximum”, correct ” At 10 °C, maximum”
in figure 6 the symbols in the graph are not sufficiently explained
r. 376: wrong ”° C; thus, isomers”, correct °C; thus, isomers
Author Response
Please see the attachment
Author Response File: 
Author Response.docx
Reviewer 2 Report
Chia (Salvia hispanica L.) seed soaking, germination and behavior of fatty acids at different temperatures
The manuscript entitled "Chia (Salvia hispanica L.) seed imbibition, germination and fatty acid behavior at different temperatures" is one of great interest due to the growing demand for chia seeds. The manuscript is well structured and well organized.
The abstract must be reformulated so as to follow the instructions required by the journal (you can find them in the Microsoft Word template - Instructions for Authors) in order to be much better understood.
Keywords: for better visibility it would be recommended that keywords be different from the words in the title
Statistical analysis is missing from table 1
The conclusions section is missing
Author Response
Please see the attachment.
Author Response File: Author Response.docx
Reviewer 3 Report
Cabrera-Santos et al. presented the effect of temperature on the germination and on the free lipid acids of Chia seeds. The topic is appropriate for Agriculture.
Line 18 revise
Lines 19-20 “stimulating the interest in their seed physiology to support agricultural Practices” revise to “stimulating the interest in their physiology to maximize the effects of specific agricultural Practices
Line 20 Here the authors should present the problem (for example reduced seed vigor or germination rate after storage at high temperatures).
Line 23 replace the term “germination parameters” with the terms used universally “seed vigor” and “seed germination”. Make appropriate adjustments throughout the manuscript.
Line 27 revise to faster germination
Line 30 Present the consequence of the changes in physicochemical properties of the membranes (apparently the seeds are stressed…)
Line 43 Please avoid the usage of the term superfood for the characterization of this product. In my opinion, it can be used only in a descriptive way for the appreciation of the product by the consumers.
Lines 53-54 The term technology needs to be revised.
Line 55. Revise the term “next-generation”
Line 59 provide reference
Line 61 revise “period time”
Lines 63-64 I am not so sure that membrane organization precedes the molecular events.
Line 67 revise “in this issue”
Lines 71 revise, this research does not examine seed aging.
Line 84. Explain signal
Line 85-86 revise
Line 87. Provide reasoning for the treatments (for example 10oC simulates germination under colder climates, 20oC is the optimum, 30oC simulates germination in an environment that can cause heat stress. However, since Chia seeds favor high temperatures (thus no stress or limited stress occur at 30oC). Please check the reasoning for this experiment and adjust accordingly throughout the paper.
Materials and Methods.
Please clarify the reasoning for the use of light during the germination experiments.
Line 128 replace with median germination time
Results
Revise the figure captions/table legends according to the MDPI instructions for authors and provide the necessary information to the reader
Please clarify the results in Table 1 and Figure 3
Suggestion: Figure 4 I am not so sure that the 3d presentation is the best way to present the results. Maybe a graph like the one in Fig 6 is more appropriate.
Discussion
Line 319 please revise
Explain the further physiological mechanism that leads to the generation of lipid acids during seed germination. Provide the significance of each of P, S, L and Ln for germination and investigate the physiological reasoning of the observed differences (there is a discussion between the lines 345-367 but must be improved).
Line 321 explain or revise different physiological stage
Remove the statement in lines 390-394.
Author Response
Please see the attachment.
Author Response File: Author Response.docx
Round 2
Reviewer 2 Report
The authors responded to all my suggestions for improvement.
Author Response
A detailed grammar and spelling check was performed. More than 300 changes were included, which significantly improves the understanding of the manuscript.
Reviewer 3 Report
After the revision the article can be accepted for publication.
Author Response
A detailed grammar and spelling check was performed. More than 300 changes were included, which significantly improves the understanding of the manuscript.
