Indirect Voltammetry Detection of Non-Electroactive Neurotransmitters Using Glassy Carbon Microelectrodes: The Case of Glutamate
Round 1
Reviewer 1 Report
The present work immobilization of L-glutamate oxidase (GluOx) enzyme on the surface of GC microelectrodes to enable catalysis of chemical reaction between L-glutamate, oxygen, and water to produce H2O2 , an electroactive byproduct that is readily detectable through voltammetry. Some issues should be adressed:
(1)The authors are suggested to use the template recommended by the journal;
(2) The quality of Fig.6 must be improved.
(3) The "-1" in Fig.6 should be superscript format.
(4) Page 11, "While prior work on the diffusion of glutamate towards carbon surfaces is missing in the literature, the demonstration of adsorption through bond formation here is an important outcome that further supports the effectiveness of GC for sensing glutamate at low detection limits.", the more detailed description is suggested to be added.
(5) Page 10, the quality of Fig. 7 and 8 must be improved, the outermost box is not needed.
Overall, the paper is well organised.
The present work immobilization of L-glutamate oxidase (GluOx) enzyme on the surface of GC microelectrodes to enable catalysis of chemical reaction between L-glutamate, oxygen, and water to produce H2O2 , an electroactive byproduct that is readily detectable through voltammetry. Some issues should be adressed:
(1)The authors are suggested to use the template recommended by the journal;
(2) The quality of Fig.6 must be improved.
(3) The "-1" in Fig.6 should be superscript format.
(4) Page 11, "While prior work on the diffusion of glutamate towards carbon surfaces is missing in the literature, the demonstration of adsorption through bond formation here is an important outcome that further supports the effectiveness of GC for sensing glutamate at low detection limits.", the more detailed description is suggested to be added.
(5) Page 10, the quality of Fig. 7 and 8 must be improved, the outermost box is not needed.
Overall, the paper is well organised.
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
Reviewer 2 Report
This paper reported indirect voltammetry detection of glutamate using glassy carbon microelectrodes. Although being interesting, I find that there are some major issues with the paper that require addressing prior to this being considered for publication in this journal.
I have identified the main points for consideration below:
1. The manuscript has some minor spelling, subscript, and grammar errors. Please carefully check the whole manuscript.
2. Some recent references related to dopamine detection are recommended to be cited in the introduction section, such as Microchemical Journal 190 (2023) 108726.
3. The anti-fouling property of electrode is essential for in-vivo detection. So, the anti-fouling property should be investigated in the revised manuscript.
4. The standard curves and corresponding LOD of the proposed microelectrode should be provided in the revised manuscript.
5. The selectivity of the proposed microelectrode should be investigated in the revised manuscript.
6.The repeatability, reproducibility, and stability of the proposed microelectrodes should be added in the revised manuscript.
7. The advantages of FSCV should be described in the revised manuscript.
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
Round 2
Reviewer 2 Report
Accept in present form.
Accept in present form.
Author Response
Thanks!