Water Buffalo Welfare, Strategies to Improve Health, Behavior, Productivity, and Food Quality and Safety

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Cattle".

Deadline for manuscript submissions: closed (31 July 2024) | Viewed by 33897

Special Issue Editor


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Guest Editor
Neurophysiology, Behavior and Animal Welfare Assessment, DPAA, Universidad Autónoma Metropolitana, Xochimilco Campus, Mexico City 04960, Mexico
Interests: animal welfare; thermoregulation
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Special Issue Information

Dear Colleagues,

This Special Issue, entitled "Water Buffalo Welfare, Strategies to Improve Health, Behavior, Productivity, and Food Quality and Safety", aims to gather and publish leading research papers on advances in the study of the water buffalo, especially in such crucial areas as infectious diseases, biotechnological applications, strategies to improve health and productivity, molecular cytogenetics, reproductive biotechnologies, animal sustainability, human–animal relationships, the bases of sustainable models, behavior and animal welfare, feeding, nutrition, and sustainability, feeding, and ruminal biochemistry, the effects of heat stress, food quality, and safety, among other topics.

According to the FAO, over the past 50 years, the number of farmed water buffaloes (Bubalus bubalis) has increased to more than that of cattle. The water buffalo is now classified as the sixth-most abundant productive species in the world, behind only stocks of poultry, bovine cattle (genus Bos), swine, sheep, and goats. In 2018, the FAO reported a worldwide population of 206 million water buffaloes, distributed in 48 countries, and highlighted a rate of population growth for this species of 11% from 2008 to 2018: a figure 6% higher than Bos cattle in the same period. The water buffalo has thus opened an opportunity to exploit a species with enormous potential for development in tropical areas, especially in zones with high humidity, since it is a rustic species that can adapt to complex habitats. Reports cite significant productive indices in intensive production systems where the water buffalo’s meat, milk, and mozzarella cheese are highly appreciated. Moreover, this species has been introduced into new environments under both intensive and extensive conditions, and breeding operations are currently spreading from Asia and Europe to other nations, including the Latin American region.

The study of the water buffalo presents a true challenge to both scientists and producers; while significant advances have been made in scientific research, firmly establishing this species in an industry that drives the economy of numerous countries, knowledge in such areas as pathologies, physiology, ethology, genetics, reproduction, zootechnics, nutrition, food quality, and safety is still lacking. Today, the water buffalo is the basis of the only value chain in agroalimentary systems with notable growth offering high returns on investment.

The objective of this Special Issue is to publish and disseminate scientific advances and technological innovations that can improve the reproductive and nutritional efficiency of this livestock species, and thus raise the productivity of Bubalus bubalis ranches, improve the animals’ state of health, optimize their level of welfare through ethology, and produce nutritious foods while, at the same time, protecting the environment and ensuring that all measures implemented into food are safe and innocuous.

I invite researchers in these fields to submit original or review manuscripts that address one or more of these diverse topics. These suggestions include, but are not limited to:

  • Biotechnological applications in water buffalo research;
  • Interactions with the environment;
  • Climate change and productivity;
  • Feeding, nutrition, and sustainability;
  • Infectious and non-infectious diseases;
  • Strategies to improve health;
  • Reproductive biotechnologies;
  • Behavior and animal welfare;
  • Food quality and safety;
  • Molecular tools and infectious disease epidemiology;
  • Genetic diversity, heterozygosity, and genetic structure;
  • Molecular cytogenetics, risk analysis, and genomic epidemiology;
  • Animal sustainability on buffalo ranches;
  • The environmental impacts of water buffalo production on different systems;
  • The human–animal relationship;
  • Sustainable systems in humid tropical zones;
  • Handling and physiological aspects;
  • Climate change and its varied impacts on productivity;
  • Infrared thermography as a tool for assessing buffalo health and welfare;
  • Acclimatization, adaptation to high temperatures, and the thermal comfort index;
  • Reproductive management;
  • Assisted reproductive biotechnologies;
  • Pregnancy, calving, lactation, weaning, confinement, pasturing, transport, lairage, and slaughter;
  • Advances in embryo production, cryopreservation, and reproductive ultrasonography;
  • Cloning and transgenesis;
  • Behavior and strategies to reduce heat stress;
  • Maternal and neonatal behavior;
  • Stereotypes and redirected behaviors;
  • Quality of death and the neurobiology of sensitization;
  • Welfare and pain at slaughter;
  • Deep unconsciousness during death, hyperalgesia, and events at slaughter;
  • Dairy and meat production;
  • Feed resources and rumen fermentation;
  • Feeding and ruminal biochemistry;
  • The use of draught animals in rural labor;
  • Transport and handling;
  • Meat, milk, and mozzarella cheese quality;
  • Food safety, shelf-life, and packaging;
  • Tracing systems and processed milk and meat products;
  • Authenticity and molecular techniques in meat, milk, and mozzarella cheese;
  • Nutritional and sensory analysis;
  • Effects of packaging type and aging on meat quality;
  • Impact of freezing on the microbiological quality of mozzarella cheese.

Dr. Daniel Mota-Rojas
Guest Editor

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Keywords

  • water buffalo
  • Bubalus bubalis
  • meat quality
  • welfare
  • behavior
  • health
  • food quality
  • food safety
  • productivity
 

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Published Papers (18 papers)

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15 pages, 1096 KiB  
Article
Study of the Milkability of the Mediterranean Italian Buffalo and the Tunisian Maghrebi Camel According to Parity and Lactation Stage
by Moufida Atigui, Marwa Brahmi, Pierre-Guy Marnet, Wiem Ben Salem, Maria Concetta Campagna, Antonio Borghese, Giuseppe Todde, Maria Caria, Mohamed Hammadi and Carlo Boselli
Animals 2024, 14(7), 1055; https://doi.org/10.3390/ani14071055 - 29 Mar 2024
Viewed by 1119
Abstract
While considered as hard milkers, both buffaloes and camels are milked with equipment destined for dairy cows based on external morphological similarities with this species. This work aimed to study similarities and differences in milkability traits between Mediterranean buffaloes and Maghrebi she-camels and [...] Read more.
While considered as hard milkers, both buffaloes and camels are milked with equipment destined for dairy cows based on external morphological similarities with this species. This work aimed to study similarities and differences in milkability traits between Mediterranean buffaloes and Maghrebi she-camels and to evaluate the effect of parity and lactation stage. A total of 422 milk flow curves recorded with an electronic milkmeter (Lactocorder®) for both species were accessed. Milking characteristics including milk yield per milking, peak milk flow, average milk flow, duration of the main milking phase, duration of total milking, duration of various phases of milk flow, lag time and time to milk ejection, stripping yield, overmilking time and incidence of bimodal milk flow curves were evaluated for both species. Results showed that the values of milk yield per milking, duration of the main milking phase and duration of total milking were higher in buffaloes (3.98 ± 0.10 kg; 4.07 ± 0.11 min; 9.89 ± 0.21 min, respectively) compared to camels (3.51 ± 0.08 kg; 3.05 ± 0.09 min; 3.76 ± 0.09 min, respectively). However, camels had significantly higher peak and average milk flow (2.45 ± 0.07 kg/min and 1.16 ± 0.03 kg/min, respectively). Camels took significantly less time for milk ejection to occur. Only 15.49% of recorded curves were bimodal in buffaloes while 34.93% of bimodal curves were recorded for camels. Overmilking was significantly higher in buffaloes (3.64 ± 0.21 min vs. 0.29 ± 0.02 min). Parity and lactation stage had a significant effect on most studied milking traits suggesting the need for some particular practices with primiparous animals and animals at different levels of lactation for both species. Full article
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12 pages, 496 KiB  
Article
Some Welfare Assessment Traits and Quantitative-Qualitative Milk Parameters as Affected by Supplementary Feeding at Milking and Parity in Anatolian Buffalo Cows
by Ahmet Akdağ, İbrahim Cihangir Okuyucu, Hüseyin Erdem, Ertuğrul Kul and Nuh Ocak
Animals 2024, 14(6), 956; https://doi.org/10.3390/ani14060956 - 19 Mar 2024
Viewed by 1036
Abstract
This study aimed to evaluate whether supplemental feeding at milking (SFAM) positively influences the quantitative−qualitative milk parameters due to improving some welfare assessment traits of multiparous Anatolian buffalo cows confined in semi-open free-stall barns. A total of 76 Anatolian buffalo cows at approximately [...] Read more.
This study aimed to evaluate whether supplemental feeding at milking (SFAM) positively influences the quantitative−qualitative milk parameters due to improving some welfare assessment traits of multiparous Anatolian buffalo cows confined in semi-open free-stall barns. A total of 76 Anatolian buffalo cows at approximately 90 days in milk were selected to encompass four groups (OSF-2nd, NSF-2nd, OSF-≥3rd and NSF-≥3rd), considering offering (OSF) or not (NSF) supplemental feed at milking and the parity (2nd) and (≥3rd). Data of evaluated variables such as the following ones—(i) subjectively scored welfare assessment traits (temperament, udder hygiene and body condition), (ii) milk yield per milking (MYM), (iii) milk components, and (iv) milk physical traits—were analysed using a linear mixed model and principal component (PC) analysis. The OSF improved the temperament, udder hygiene and body condition scores compared to the NSF. The MYM, the fat content and the fat-to-protein ratio of the OSF were higher than those of the NSF, but milk mineral and electrical conductivity of the OSF were lower than those of the NSF. The parity of cows did not affect the evaluated variables. Four parameters (milk density value and lactose, solids-not-fat and protein contents) could be identified in the PC2 versus PC1 plot. In conclusion, the SFAM enhanced the milk yield and qualitative milk parameters due to improving the welfare status of indoor buffalo cows, regardless of parity. Full article
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9 pages, 425 KiB  
Communication
Evaluation of Anatolian Water Buffalo Carcass Weights Based on a Slaughterhouse Data Collection
by Nursen Ozturk, Sevinc Arap, Omur Kocak, Lorenzo Serva, Kozet Avanus, Halil Ibrahim Kilic, Luisa Magrin and Halil Gunes
Animals 2024, 14(5), 710; https://doi.org/10.3390/ani14050710 - 24 Feb 2024
Viewed by 1161
Abstract
This study analyzed data collected on a slaughterhouse from 2017 to 2021, belonging to five hundred and twenty one Anatolian water buffalos from different farms located in Edirne, Istanbul, and Kirklareli. Specifically, it aimed to determine the factors affecting the carcass weights and [...] Read more.
This study analyzed data collected on a slaughterhouse from 2017 to 2021, belonging to five hundred and twenty one Anatolian water buffalos from different farms located in Edirne, Istanbul, and Kirklareli. Specifically, it aimed to determine the factors affecting the carcass weights and slaughter ages of the Anatolian water buffalos. The results of the study showed that the slaughter age of the buffalos was a significant determinant of their carcass weights. Meanwhile, the sex, slaughter year, and slaughter season affected the carcass weight. Differences were observed for the slaughter age regarding the sex and farm origin. Since the pricing system in local markets is based on the buffalo carcass weight, the findings of this study could be essential for farmers when determining their fattening strategies. Full article
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14 pages, 2847 KiB  
Article
Prediction of Body Weight by Using PCA-Supported Gradient Boosting and Random Forest Algorithms in Water Buffaloes (Bubalus bubalis) Reared in South-Eastern Mexico
by Armando Gomez-Vazquez, Cem Tırınk, Alvar Alonzo Cruz-Tamayo, Aldenamar Cruz-Hernandez, Enrique Camacho-Pérez, İbrahim Cihangir Okuyucu, Hasan Alp Şahin, Dany Alejandro Dzib-Cauich, Ömer Gülboy, Ricardo Alfonso Garcia-Herrera and Alfonso J. Chay-Canul
Animals 2024, 14(2), 293; https://doi.org/10.3390/ani14020293 - 17 Jan 2024
Cited by 1 | Viewed by 1500
Abstract
This study aims to use advanced machine learning techniques supported by Principal Component Analysis (PCA) to estimate body weight (BW) in buffalos raised in southeastern Mexico and compare their performance. The first stage of the current study consists of body measurements and the [...] Read more.
This study aims to use advanced machine learning techniques supported by Principal Component Analysis (PCA) to estimate body weight (BW) in buffalos raised in southeastern Mexico and compare their performance. The first stage of the current study consists of body measurements and the process of determining the most informative variables using PCA, a dimension reduction method. This process reduces the data size by eliminating the complex structure of the model and provides a faster and more effective learning process. As a second stage, two separate prediction models were developed with Gradient Boosting and Random Forest algorithms, using the principal components obtained from the data set reduced by PCA. The performances of both models were compared using R2, RMSE and MAE metrics, and showed that the Gradient Boosting model achieved a better prediction performance with a higher R2 value and lower error rates than the Random Forest model. In conclusion, PCA-supported modeling applications can provide more reliable results, and the Gradient Boosting algorithm is superior to Random Forest in this context. The current study demonstrates the potential use of machine learning approaches in estimating body weight in water buffalos, and will support sustainable animal husbandry by contributing to decision making processes in the field of animal science. Full article
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16 pages, 280 KiB  
Article
Agroecological Zone-Specific Diet Optimization for Water Buffalo (Bubalus bubalis) through Nutritional and In Vitro Fermentation Studies
by Sultan Singh, Pushpendra Koli, B. P. Kushwaha, Uchenna Y. Anele, Sumana Bhattacharya and Yonglin Ren
Animals 2024, 14(1), 143; https://doi.org/10.3390/ani14010143 - 31 Dec 2023
Viewed by 1133
Abstract
The water buffalo faces challenges in optimizing nutrition due to varying local feed resources. In response to this challenge, the current study introduces originality by addressing the lack of region-specific feeding strategies for water buffaloes. This is achieved through the formulation of 30 [...] Read more.
The water buffalo faces challenges in optimizing nutrition due to varying local feed resources. In response to this challenge, the current study introduces originality by addressing the lack of region-specific feeding strategies for water buffaloes. This is achieved through the formulation of 30 different diets based on locally available resources, offering a tailored approach to enhance nutritional optimization in diverse agroecological contexts. These diets were segmented into three groups of ten, each catering to the maintenance (MD1 to MD10), growth (GD1 to GD10), and lactation/production (PD1 to PD10) needs of buffaloes. Utilizing local feed ingredients, each diet was assessed for its chemical composition, in vitro gas and methane emissions, and dry matter (DM) disappearance using buffalo rumen liquor. The production diets (127 and 32.2 g/kg DM) had more protein and fats than the maintenance diets (82.0 and 21.0 g/kg DM). There was less (p < 0.05) fiber in the production diets compared to the maintenance ones. Different protein components (PB1, PB2) were lower (p < 0.05) in the maintenance diets compared to the growth and production ones, but other protein fractions (PB3, Pc) were higher (p < 0.05) in the maintenance diet. Furthermore, the growth diets had the highest amount of other protein components (PA), while the maintenance diets had the highest amount of soluble carbohydrates (586 g/kg DM), whereas the carbohydrate fraction (CB1) was highest (p < 0.05) in the production diets (187 g/kg DM), followed by the growth (129 g/kg DM) and maintenance diets (96.1 g/kg DM). On the contrary, the carbohydrate CA fraction was (p < 0.05) higher in the maintenance diets (107 g/kg DM) than in the growth (70.4 g/kg DM) and production diets (44.7 g/kg DM). The in vitro gas production over time (12, 24, and 48 h) was roughly the same for all the diets. Interestingly, certain components (ether extract, lignin, NDIN, ADIN, and PB3 and CC) of the diets seemed to reduce methane production, while others (OM, NPN, SP, PA and PB1, tCHO and CB2) increased it. In simple words, this study reveals that different diets affect gas production during digestion, signifying a significant step towards a promising future for buffalo farming through tailored, region-specific formulations. Full article
13 pages, 3476 KiB  
Article
Optimising Electroporation Condition for CRISPR/Cas-Mediated Knockout in Zona-Intact Buffalo Zygotes
by Meeti Punetha, Dharmendra Kumar, Sheetal Saini, Suman Chaudhary, Kamlesh Kumari Bajwa, Surabhi Sharma, Manu Mangal, Prem S. Yadav, Jonathan A. Green, Kristin Whitworth and Tirtha K. Datta
Animals 2024, 14(1), 134; https://doi.org/10.3390/ani14010134 - 30 Dec 2023
Cited by 5 | Viewed by 1520
Abstract
Somatic cell nuclear transfer or cytoplasm microinjection has widely been used to produce genome-edited farm animals; however, these methods have several drawbacks which reduce their efficiency. In the present study, we describe an easy adaptable approach for the introduction of mutations using CRISPR-Cas9 [...] Read more.
Somatic cell nuclear transfer or cytoplasm microinjection has widely been used to produce genome-edited farm animals; however, these methods have several drawbacks which reduce their efficiency. In the present study, we describe an easy adaptable approach for the introduction of mutations using CRISPR-Cas9 electroporation of zygote (CRISPR-EP) in buffalo. The goal of the study was to determine the optimal conditions for an experimental method in which the CRISPR/Cas9 system is introduced into in vitro-produced buffalo zygotes by electroporation. Electroporation was performed using different combinations of voltage, pulse and time, and we observed that the electroporation in buffalo zygote at 20 V/mm, 5 pulses, 3 msec at 10 h post insemination (hpi) resulted in increased membrane permeability and higher knockout efficiency without altering embryonic developmental potential. Using the above parameters, we targeted buffalo POU5F1 gene as a proof of concept and found no variations in embryonic developmental competence at cleavage or blastocyst formation rate between control, POU5F1-KO, and electroporated control (EC) embryos. To elucidate the effect of POU5F1-KO on other pluripotent genes, we determined the relative expression of SOX2, NANOG, and GATA2 in the control (POU5F1 intact) and POU5F1-KO-confirmed blastocyst. POU5F1-KO significantly (p ≤ 0.05) altered the expression of SOX2, NANOG, and GATA2 in blastocyst stage embryos. In conclusion, we standardized an easy and straightforward protocol CRISPR-EP method that could be served as a useful method for studying the functional genomics of buffalo embryos. Full article
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13 pages, 2249 KiB  
Article
Effects of Climatic Conditions and Supplementation with Palm Cake on the Thermoregulation of Crossbred Buffaloes Raised in a Rotational Grazing System and with Natural Shade in Humid Tropical Regions
by Carolina Carvalho Brcko, Jamile Andrea Rodrigues da Silva, Alexandre Rossetto Garcia, André Guimarães Maciel e Silva, Lucieta Guerreiro Martorano, Reíssa Alves Vilela, Benjamim de Souza Nahúm, Antônio Vinícius Corrêa Barbosa, Welligton Conceição da Silva, Thomaz Cyro Guimarães de Carvalho Rodrigues, Éder Bruno Rebelo da Silva and José de Brito Lourenço-Júnior
Animals 2024, 14(1), 53; https://doi.org/10.3390/ani14010053 - 22 Dec 2023
Cited by 1 | Viewed by 1026
Abstract
In ruminants, diet composition has a positive correlation with heat production, which can influence thermoregulation, energy expenditure and, consequently, animal performance. The objective of this work was to evaluate the effects of climatic conditions and supplementation based on palm kernel cake, on the [...] Read more.
In ruminants, diet composition has a positive correlation with heat production, which can influence thermoregulation, energy expenditure and, consequently, animal performance. The objective of this work was to evaluate the effects of climatic conditions and supplementation based on palm kernel cake, on the thermoregulation of crossbred buffaloes in the eastern Amazon. The research was carried out at Embrapa Amazônia Oriental (01°26′ S and 48°24′ W), Belém, Pará, and lasted 12 months (representing the entire year). Twenty-four buffaloes, females, with initial age and an average weight of 54 ± 7 months and 503.1 ± 23 kg, respectively, non-pregnant, non-lactating and clinically healthy were used, divided into four treatments based on the supplementation content of the palm cake (%DM) in relation to their body weight (%): 0, 0.25, 0.50 and 1.0. The animals were kept in paddocks with Brachiaria brizantha (cv. Marandu), in a rotating system, with water to drink and mineral salt ad libitum. Equipment was installed to record environmental data (temperature and relative humidity, dew point temperature, wet bulb and black globe) and physiological data: rectal temperature (RT); respiratory rate (RR); and body surface temperature (BST), recorded twice a day, always in the morning (6:00 a.m. to 7:00 a.m.) and afternoon (12:00 p.m. to 1:00 p.m.) shifts, and were used to calculate the Globe Temperature and Humidity Index (GTHI). Supplementation did not influence the physiological variables of thermoregulation (p > 0.05). However, there were differences in the GTHI between the shifts, with higher means in the afternoon shift, especially in the less rainy period of the year, where the GTHI reached 92.06 ± 2.74 (p < 0.05). In all periods of the year, the mean values of RT, RR and BST were higher in the afternoon shift (p > 0.05). The respiratory rate (RR) is associated with the annual seasonality of the thermal waters, with higher averages in the afternoons of the rainy season. The positive correlation for rectal temperature, respiratory rate and body surface temperature indicated that buffaloes respond to thermal elevations in the atmosphere (afternoon period) and, consequently, reflect on the GTHI. Supplementation does not influence thermoregulation; the changes observed occurred in response to the region’s thermal and rainfall conditions (mainly in the afternoon shift), with higher GTHI values. Full article
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18 pages, 2875 KiB  
Article
Characterization of the Ruminal Microbiome of Water Buffaloes (Bubalus bubalis) Kept in Different Ecosystems in the Eastern Amazon
by Gerlane Nunes Noronha, Melanie K. Hess, Ken G. Dodds, André Guimarães Maciel e Silva, Shirley Motta de Souza, Jamile Andréa Rodrigues da Silva, Diego Assis das Graças, Thomaz Cyro Guimarães de Carvalho Rodrigues, Welligton Conceição da Silva, Éder Bruno Rebelo da Silva, Peter H. Janssen, Hannah M. Henry, Suzanne J. Rowe, Vinicius Costa Gomes de Castro and José de Brito Lourenço-Júnior
Animals 2023, 13(24), 3858; https://doi.org/10.3390/ani13243858 - 15 Dec 2023
Viewed by 1589
Abstract
Increasing the efficiency of rumen fermentation is one of the main ways to maximize the production of ruminants. It is therefore important to understand the ruminal microbiome, as well as environmental influences on that community. However, there are no studies that describe the [...] Read more.
Increasing the efficiency of rumen fermentation is one of the main ways to maximize the production of ruminants. It is therefore important to understand the ruminal microbiome, as well as environmental influences on that community. However, there are no studies that describe the ruminal microbiota in buffaloes in the Amazon. The objective of this study was to characterize the rumen microbiome of the water buffalo (Bubalus bubalis) in the eastern Amazon in the dry and rainy seasons in three grazing ecosystems: Baixo Amazonas (BA), Continente do Pará (CP), Ilha do Marajó (IM), and in a confinement system: Tomé-Açu (TA). Seventy-one crossbred male buffaloes (Murrah × Mediterranean) were used, aged between 24 and 36 months, with an average weight of 432 kg in the rainy season and 409 kg in the dry season, and fed on native or cultivated pastures. In the confinement system, the feed consisted of sorghum silage, soybean meal, wet sorghum premix, and commercial feed. Samples of the diet from each ecosystem were collected for bromatological analysis. The collections of ruminal content were carried out in slaughterhouses, with the rumen completely emptied and homogenized, the solid and liquid fractions separated, and the ruminal pH measured. DNA was extracted from the rumen samples, then sequenced using Restriction Enzyme Reduced Representation Sequencing. The taxonomic composition was largely similar between ecosystems. All 61 genera in the reference database were recognized, including members of the domains Bacteria and Archaea. The abundance of 23 bacterial genera differed significantly (p < 0.01) between the Tomé-Açu confinement and other ecosystems. Bacillus, Ruminococcus, and Bacteroides had lower abundance in samples from the Tomé-Açu system. Among the Archaea, the genus Methanomicrobium was less abundant in Tomé-Açu, while Methanosarcina was more abundant. There was a difference caused by all evaluated factors, but the diet (available or offered) was what most influenced the ruminal microbiota. Full article
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17 pages, 391 KiB  
Article
Evaluation of the Composition of the Cholesterol, Tocopherols, β-Carotene and Fatty Acids Profile of the Liver Tissue of Male Water Buffaloes (Bubalus bubalis) Managed in Different Ecosystems of the Eastern Amazon
by Laurena Silva Rodrigues, Jamile Andrea Rodrigues da Silva, José de Brito Lourenço-Júnior, André Guimarães Maciel e Silva, Thomaz Cyro Guimarães de Carvalho Rodrigues, Welligton Conceição da Silva, Thiago Carvalho da Silva, Vinicius Costa Gomes de Castro, Cristina Mateus Alfaia, André Martinho de Almeida and José António Mestre Prates
Animals 2023, 13(24), 3785; https://doi.org/10.3390/ani13243785 - 8 Dec 2023
Cited by 2 | Viewed by 1667
Abstract
The diet offered to animals has a great influence on the composition of tissues and, consequently, the quality. The objective of this study was to evaluate the influence of Amazonian ecosystems, in the dry and rainy periods of the year, on the composition [...] Read more.
The diet offered to animals has a great influence on the composition of tissues and, consequently, the quality. The objective of this study was to evaluate the influence of Amazonian ecosystems, in the dry and rainy periods of the year, on the composition of cholesterol, tocopherols, β-carotene and the fatty acid profile of the livers of water buffaloes (Bubalus bubalis) reared in the Eastern Amazon, in an extensive or intensive system. Total lipid content was influenced by the location and time of year (p < 0.05). Ninety-six male water buffaloes were used (12 per sampling period), aged between 24 and 36 months, with average weights of 432 kg (end of the rainy season) and 409 kg (end of the dry season). Total cholesterol, α-tocopherol and γ-tocopherol influenced the relationship between extensive vs intensive ecosystems, location, periods and the interaction between the location and period of the year (p < 0.05). Animals raised in a pasture ecosystem had the highest values of omega-3, and those raised in confinement, the highest values of omega-6 (p < 0.05). The proportions of n-6/n-3 and hypocholesterolemia (7.14) and hypercholesterolemia (3.08%) (h/H) were found in greater amounts in animals raised in confinement (p < 0.05). The atherogenic index (AI) had a higher value in the rainy season, in animals raised in Santarém (2.37%), with no difference between pasture and feedlot ecosystems, except in animals raised in the rainy season in Nova Timboteua, with a lower AI (1.53%). The thrombogenicity index (TI) was higher in the livers of confined animals (0.32%) and lower (0.18%) in those raised in Nova Timboteua (rainy season). Amazonian ecosystems influence the nutritional values of buffalo liver, with the best nutritional values in animals in the extensive system. Full article
12 pages, 878 KiB  
Article
Heat Stress Mitigation: Impact of Increased Cooling Sessions on Milk Yield and Welfare of Dairy Buffaloes in a Semiarid Summer
by Syed Israr Hussain, Nisar Ahmad, Saeed Ahmed, Maqsood Akhter and Muhammad Qamer Shahid
Animals 2023, 13(21), 3315; https://doi.org/10.3390/ani13213315 - 25 Oct 2023
Cited by 2 | Viewed by 1570
Abstract
The current study aimed to evaluate the impact of increasing cooling sessions from three to five times a day on milk yield and the welfare of dairy buffaloes during a semiarid summer in Pakistan. Eighteen Nili Ravi buffaloes were randomly assigned to three [...] Read more.
The current study aimed to evaluate the impact of increasing cooling sessions from three to five times a day on milk yield and the welfare of dairy buffaloes during a semiarid summer in Pakistan. Eighteen Nili Ravi buffaloes were randomly assigned to three cooling strategies: (1) CTL, where buffaloes were cooled with a handheld hosepipe twice daily for 5 min each; (2) 3CS, where buffaloes were cooled using sprinklers three times daily; and (3) 5CS, where buffaloes were cooled using sprinklers five times daily. Each sprinkler cooling session lasted 1 h, with a 6 min cycle of 3 min of water on and 3 min off. Results showed that the 5CS group produced 1.6 and 3.2 kg more milk per day compared to the 3CS and CTL groups, respectively (p < 0.001). Both the 5CS and 3CS groups had consistently lower core body temperatures and respiration rates than the CTL group. Buffaloes in the 5CS group spent significantly more time eating (p < 0.001). Additionally, the 5CS group exhibited lower cortisol and blood urea nitrogen levels (p = 0.001) and higher glucose levels than the CTL group (p = 0.006). In conclusion, increasing cooling sessions to five times daily improved milk yield and welfare compared to the traditional cooling strategy (CTL) in dairy buffaloes during semiarid summers, highlighting the benefits of optimized cooling practices. Full article
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18 pages, 3832 KiB  
Article
Thermal Balance in Male Water Buffaloes Transported by Long and Short Journeys
by Daniela Rodríguez-González, Isabel Guerrero Legarreta, Alfonso Chay-Canul, Ismael Hernández-Avalos, Fabio Napolitano, Ricardo García-Herrera, Alfredo M. F. Pereira, Adriana Domínguez-Oliva, Alejandro Casas-Alvarado, Brenda Reyes-Sotelo and Daniel Mota-Rojas
Animals 2023, 13(20), 3274; https://doi.org/10.3390/ani13203274 - 20 Oct 2023
Cited by 1 | Viewed by 1353
Abstract
Transport is a stressor that can cause physiological and metabolic imbalances in livestock, resulting in stress-induced hyperthermia. In water buffaloes, studies regarding the thermal state of animals during mobilization are scarce. Therefore, this study aimed to compare the thermal response of 1516 water [...] Read more.
Transport is a stressor that can cause physiological and metabolic imbalances in livestock, resulting in stress-induced hyperthermia. In water buffaloes, studies regarding the thermal state of animals during mobilization are scarce. Therefore, this study aimed to compare the thermal response of 1516 water buffaloes using infrared thermography (IRT) during 15 short trips (783 animals, 60,291 records, average duration = 50.33 min ± 5.48 min) and 14 long trips (733 animals, 56,441 records, average duration = 13.31 h ± 47.32 min). The surface temperature was assessed in 11 regions (periocular, lacrimal caruncle, nasal, lower eyelid, auricular, frontal-parietal, pelvic limb, torso, abdominal, lumbar, and thoracic) during seven phases from pasture to post-transport. It was found that the surface temperature of the periocular, lacrimal caruncle, nasal, auricular, frontal-parietal, pelvic limb, torso, abdominal, lumbar, and thoracic regions was significantly higher during SJs (+3 °C) when compared to LJs (p < 0.0001). In particular, the frontal-parietal region had a significant increase of 10 °C during the post-transport phase (p < 0.0001) in both groups, recording the highest temperatures during this phase. Likewise, a strong positive significant correlation between the different regions was found (r = 0.90, p < 0.0001). It is worth mentioning that the herding, loading, pre-, and post-transport phases were the ones where the greatest thermal response was recorded, possibly due to the influence of human interaction. Finally, a strong positive correlation (r above 0.9, p > 0.001) between the periocular, lacrimal caruncle, pinna, and pelvic limb was found. According to the results, SJ could be considered a stressful event that hinders thermal generation, contrarily to LJ. Full article
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12 pages, 270 KiB  
Review
Degnala in Water Buffaloes: A Review on a Neglected Disease
by André de Medeiros Costa Lins and Felipe Masiero Salvarani
Animals 2024, 14(16), 2292; https://doi.org/10.3390/ani14162292 - 6 Aug 2024
Viewed by 1201
Abstract
Degnala is one of the primary mycotoxicoses affecting buffaloes, with Fusarium spp. as the main causative agent. This disease is strongly associated with the feeding of rice straw to buffaloes and is considered endemic to regions where rice is cultivated. Cases are concentrated [...] Read more.
Degnala is one of the primary mycotoxicoses affecting buffaloes, with Fusarium spp. as the main causative agent. This disease is strongly associated with the feeding of rice straw to buffaloes and is considered endemic to regions where rice is cultivated. Cases are concentrated in winter when conditions favor fungal growth in inadequately stored straw. Degnala is characterized by necrosis and gangrene of the extremities, including the tail, lower limbs, ears, tongue, muzzle, and teats. The pelvic limbs are more affected than the thoracic limbs. A tortuous appearance of the tail is very common, and cracks or corneal loss of the hooves may occur, exposing the blades and even the bones. There is no diagnostic method for identifying the disease in animals other than clinical and epidemiological criteria, combined with fungal culture samples. There is no treatment that neutralizes the effects of the toxins; the current treatment is palliative and supportive, consisting of pentasulfate solution, anti-Degnala liquid, 2% nitroglycerin ointment, and broad-spectrum antibiotics for secondary infections. Additionally, the management of drying and proper storage of straw is essential for controlling this disease. Full article
18 pages, 3503 KiB  
Review
A Review of the Nutritional Aspects and Composition of the Meat, Liver and Fat of Buffaloes in the Amazon
by Laurena Silva Rodrigues, Jamile Andrea Rodrigues da Silva, Welligton Conceição da Silva, Éder Bruno Rebelo da Silva, Tatiane Silva Belo, Carlos Eduardo Lima Sousa, Thomaz Cyro Guimarães de Carvalho Rodrigues, André Guimarães Maciel e Silva, José António Mestre Prates and José de Brito Lourenço-Júnior
Animals 2024, 14(11), 1618; https://doi.org/10.3390/ani14111618 - 29 May 2024
Viewed by 1084
Abstract
Thus, this review aims to deepen the understanding of buffalo farming in the Amazon, presenting the quality and nutritional value of buffalo meat and liver. This information serves as a subsidy to improve practices related to the breeding system, nutrition, health and sustainability [...] Read more.
Thus, this review aims to deepen the understanding of buffalo farming in the Amazon, presenting the quality and nutritional value of buffalo meat and liver. This information serves as a subsidy to improve practices related to the breeding system, nutrition, health and sustainability associated with aquatic buffaloes. For this, a review of the databases was carried out using the descriptors “nutritional value of buffalo meat”, “nutritional value of buffalo liver” and “buffalo breeding in the Amazon”. Thus, the consumption of foods derived from aquatic buffaloes has important nutritional value for human consumption. In view of this, it is possible to conclude that the nutrition of these animals is influenced by the biodiversity of the Amazon, giving unique characteristics to its products, also highlighting the importance of carrying out research that aims to value the potential use of this species and strengthen the economy of the region. Full article
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21 pages, 3591 KiB  
Review
Anatomy and Physiology of Water Buffalo Mammary Glands: An Anatomofunctional Comparison with Dairy Cattle
by Daniel Mota-Rojas, Fabio Napolitano, Alfonso Chay-Canul, Marcelo Ghezzi, Ada Braghieri, Adriana Domínguez-Oliva, Andrea Bragaglio, Adolfo Álvarez-Macías, Adriana Olmos-Hernández, Giuseppe De Rosa, Ricardo García-Herrera, Pamela Lendez, Corrado Pacelli, Aldo Bertoni and Vittoria Lucia Barile
Animals 2024, 14(7), 1066; https://doi.org/10.3390/ani14071066 - 30 Mar 2024
Cited by 2 | Viewed by 4154
Abstract
The present review aims to analyze the anatomical and physiological characteristics of the mammary gland and udders of water buffalo by making an anatomofunctional comparison with dairy cattle. It will also discuss the knowledge generated around the physiological regulation of milk ejection in [...] Read more.
The present review aims to analyze the anatomical and physiological characteristics of the mammary gland and udders of water buffalo by making an anatomofunctional comparison with dairy cattle. It will also discuss the knowledge generated around the physiological regulation of milk ejection in the water buffalo. It was found that buffalo’s average udder depth and width is approximately 20 cm smaller than Bos cattle. One of the main differences with dairy cattle is a longer teat canal length (around 8.25–11.56 cm), which highly influences buffalo milking. In this sense, a narrower teat canal (2.71 ± 0.10 cm) and thicker sphincter muscle are associated with needing higher vacuum levels when using machine milking in buffalo. Moreover, the predominant alveolar fraction of water buffalo storing 90–95% of the entire milk production is another element that can be related to the lower milk yields in buffalo (when compared to Bos cattle) and the requirements for prolonged prestimulation in this species. Considering the anatomical characteristics of water buffalo’s udder could help improve bubaline dairy systems. Full article
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24 pages, 1372 KiB  
Review
Viral Diseases in Water Buffalo (Bubalus bubalis): New Insights and Perspectives
by Julio Martínez-Burnes, Hugo Barrios-García, Verónica Carvajal-de la Fuente, Belkis Corona-González, Dasiel Obregón Alvarez and Dora Romero-Salas
Animals 2024, 14(6), 845; https://doi.org/10.3390/ani14060845 - 9 Mar 2024
Cited by 1 | Viewed by 2744
Abstract
The water buffalo (Bubalus bubalis) has great adaptability to rustic environments and more variable conditions than cattle, who generally share the habitat. Diseases carried by buffaloes are relatively unknown and ignored and could be transmissible; an imbalance occurs between pathogens, environment, [...] Read more.
The water buffalo (Bubalus bubalis) has great adaptability to rustic environments and more variable conditions than cattle, who generally share the habitat. Diseases carried by buffaloes are relatively unknown and ignored and could be transmissible; an imbalance occurs between pathogens, environment, and susceptible hosts, generating a severe animal health problem. Also relevant is the effect of climate change on the populations of vectors that transmit viral diseases. The discovery of new virus variants that can pass from bovine (Bos) to buffalo or vice versa or to humans has highlighted the relevance of viruses crossing the host barrier. This review discusses the clinical viral diseases most reported in the water buffalo, characteristics, epidemiology, and recent findings about disease behavior, interaction with other species, the host, vectors, and pathogens. Diseases reviewed include Foot and Mouth Disease, Rinderpest, Malignant Catarrhal Fever, Infectious Bovine Rhinotracheitis, Bovine Viral Diarrhea, and Rabies. Also, vector-borne diseases include Lumpy Skin Disease, Ephemeral Fever, and Blue Tongue. The review also considers emerging viruses such as Buffalo Pox and Schmallenberg and, finally, other viruses such as papillomatosis. The knowledge and epidemiology of buffalo viral diseases must be constantly reconsidered and updated for adequate prevention and control programs. Full article
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22 pages, 3021 KiB  
Review
The Challenge of Global Warming in Water Buffalo Farming: Physiological and Behavioral Aspects and Strategies to Face Heat Stress
by Fabio Napolitano, Giuseppe De Rosa, Alfonso Chay-Canul, Adolfo Álvarez-Macías, Alfredo M. F. Pereira, Andrea Bragaglio, Patricia Mora-Medina, Daniela Rodríguez-González, Ricardo García-Herrera, Ismael Hernández-Ávalos, Adriana Domínguez-Oliva, Corrado Pacelli, Emilio Sabia, Alejandro Casas-Alvarado, Brenda Reyes-Sotelo and Ada Braghieri
Animals 2023, 13(19), 3103; https://doi.org/10.3390/ani13193103 - 5 Oct 2023
Cited by 10 | Viewed by 3096
Abstract
Water buffaloes have morphological and behavioral characteristics for efficient thermoregulation. However, their health, welfare, and productive performance can be affected by GW. The objective of this review was to analyze the adverse effects of GW on the productive behavior and health of water [...] Read more.
Water buffaloes have morphological and behavioral characteristics for efficient thermoregulation. However, their health, welfare, and productive performance can be affected by GW. The objective of this review was to analyze the adverse effects of GW on the productive behavior and health of water buffaloes. The physiological, morphological, and behavioral characteristics of the species were discussed to understand the impact of climate change and extreme meteorological events on buffaloes’ thermoregulation. In addition, management strategies in buffalo farms, as well as the use of infrared thermography as a method to recognize heat stress in water buffaloes, were addressed. We concluded that heat stress causes a change in energy mobilization to restore animal homeostasis. Preventing hyperthermia limits the physiological, endocrine, and behavioral changes so that they return to thermoneutrality. The use of fans, sprinklers, foggers, and natural sources of water are appropriate additions to current buffalo facilities, and infrared thermography could be used to monitor the thermal states of water buffaloes. Full article
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16 pages, 4353 KiB  
Review
Slaughtering of Water Buffalo (Bubalus bubalis) with and without Stunning: A Focus on the Neurobiology of Pain, Hyperalgesia, and Sensitization
by Temple Grandin, Antonio Velarde, Ana Strappini, Marien Gerritzen, Marcelo Ghezzi, Julio Martínez-Burnes, Ismael Hernández-Ávalos, Adriana Domínguez-Oliva, Alejandro Casas-Alvarado and Daniel Mota-Rojas
Animals 2023, 13(15), 2406; https://doi.org/10.3390/ani13152406 - 25 Jul 2023
Cited by 6 | Viewed by 2430
Abstract
The slaughter process in livestock is considered a stressor where the transport and handling of animals, as well as the selected stunning and bleeding methods, can cause acute pain, distress, and suffering. In water buffaloes, although stunning is known to be performed before [...] Read more.
The slaughter process in livestock is considered a stressor where the transport and handling of animals, as well as the selected stunning and bleeding methods, can cause acute pain, distress, and suffering. In water buffaloes, although stunning is known to be performed before bleeding to induce unconsciousness, no emphasis is made on the nociceptive events during this process. Particularly, current mechanical stunning methods applied to cattle are unsuitable for water buffaloes due to anatomical differences in the skull from other large ruminants. Furthermore, although very high-pressure pneumatic (200–220 psi) may be effective in the frontal position for lighter-weight water buffalos, for heavier animals, it is less likely to be effective. The present review aims: (1) to analyze the anatomical particularities of water buffaloes to discuss the importance of selecting a stunning method suitable for buffaloes, and (2) to revise the potential pain-related consequences, such as hyperalgesia and sensitization, and the signs to assess the stun quality and death to comprehend the relevance of a proper technique according to the species. Full article
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31 pages, 8827 KiB  
Review
Strategies and Mechanisms of Thermal Compensation in Newborn Water Buffaloes
by Daniel Mota-Rojas, Ada Braghieri, Marcelo Ghezzi, María Carolina Ceriani, Julio Martínez-Burnes, Pamela Anahí Lendez, Alfredo M. F. Pereira, Karina Lezama-García, Adriana Domínguez-Oliva, Alejandro Casas-Alvarado, Emilio Sabia, Corrado Pacelli and Fabio Napolitano
Animals 2023, 13(13), 2161; https://doi.org/10.3390/ani13132161 - 30 Jun 2023
Cited by 2 | Viewed by 2232
Abstract
Hypothermia is one of the principal causes of perinatal mortality in water buffaloes and can range from 3% to 17.9%. In ruminants, factors affecting hypothermia in newborns may be of intrinsic (e.g., level of neurodevelopment, birth weight, vitality score, amount of brown fat, [...] Read more.
Hypothermia is one of the principal causes of perinatal mortality in water buffaloes and can range from 3% to 17.9%. In ruminants, factors affecting hypothermia in newborns may be of intrinsic (e.g., level of neurodevelopment, birth weight, vitality score, amount of brown fat, skin features) or extrinsic origin (e.g., maternal care, environmental conditions, colostrum consumption). When newborn buffaloes are exposed to cold stress, thermoregulatory mechanisms such as peripheral vasoconstriction and shivering and non-shivering thermogenesis are activated to prevent hypothermia. Due to the properties of infrared thermography (IRT), as a technique that detects vasomotor changes triggered by a reduction in body temperature, evaluating the central and peripheral regions in newborn buffaloes is possible. This review aims to analyze behavioral, physiological, and morphological strategies and colostrum consumption as thermal compensation mechanisms in newborn water buffalo to cope with environmental changes affecting thermoneutrality. In addition, the importance of monitoring by IRT to identify hypothermia states will be highlighted. Going deeper into these topics related to the water buffalo is essential because, in recent years, this species has become more popular and is being bred in more geographic areas. Full article
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