Reproductive Physiology of Small Ruminants

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Animal Reproduction".

Deadline for manuscript submissions: closed (28 February 2022) | Viewed by 23041

Special Issue Editor


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Guest Editor
Instituto Nacional de Investigação Agrária e Veterinária, Quinta da Fonte Boa, 2005-048 Vale de Santarém, Portugal
Interests: small ruminants; reproduction physiology and biotechnologies; andrology; reproductive efficiency and methods; reproduction parameters; sperm cryopreservation (new extenders, tecniques, new methods, etc.); cervical artificial insemination
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Dear Colleagues,

Small ruminants are the basis of many agro-ecologic systems and represent the principal revenues of many rural populations. They contribute to the fixations of populations in many areas where many agricultural systems are not possible due to poor-quality soils, insufficient water supply for irrigation, or the orography of the territory where the utilization of machinery is not possible. These systems are usually extensive but there are many areas where it is possible to select semi-intensive or intensive production systems. Additionally, small ruminants may produce milk, meat, and in some sheep breeds, wool of high quality. Native breeds usually produce products of high quality but are usually less productive than exotic breeds. Native breeds represent an irreplaceable genetic heritage which we must preserve, as a public obligation of all countries. Unfortunately major native breeds are endangered, and some of them are at risk of extinction. In some countries, some native breeds are already extinct.

Therefore, it is imperative to make great efforts to preserve native breeds. Additionally, some countries have been working for 20 years to increase the reproductive efficiency of their native breeds, which is the basis of any production system. It is also essential to increase the level of productivity—namely, milk and/or meat. We must increase and apply our knowledge about all factors that affect small ruminants’ productivity—namely, genetics, nutrition, reproduction, management, and health. Only with increased reproductive efficiency and higher productivity (milk and/or meat), alongside higher product quality, it is possible to sustainably increase revenues.

Reproductive efficiency is critical to economic viability for small ruminant exploitation. However, few producers take advantage of the available reproductive technologies (ARTs) associated with the genetic improvement that is essential to the economic sustainability of their exploitations. Today, in some countries, with the use of specialized breeds, nutrition, reproduction, and management techniques, we see increasing and sustainable levels of milk and meat productivity associated with better quality of their productions.

The use of estrous synchronization associated with artificial insemination schedules using refrigerated and frozen semen (goats) and refrigerated semen (sheep) has increased significantly since 20 years ago. In the last 10 years, more meat exploitation operations are using ARTs associated with genetic improvements to increase the productivity and quality of their products.

Other relevant factors include the preservation and improvement of small ruminant productivity. Preservation of male and female gametes determine steps. At present, the refinement of cryopreservation techniques is enabling the implementation of national germplasm banks. Additionally, frozen semen of the highest quality from high-quality genetic sires, associated with the improvement of artificial insemination programs, is steadily increasing in small ruminant exploitations. Therefore, the refinement and adequate implementation of ART technologies is essential.

The use of superior genetic males and the selection and utilization of assisted reproductive technologies (ARTs) are some of the determinant factors that we must consider to increase reproduction efficiency, productivity, and quality in the exploitation of small ruminants.

Dr. João Pedro de Sousa Santa Clara Barbas
Guest Editor

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Keywords

  • reproductive efficiency
  • productivity
  • ART
  • gametes preservation
  • reproduction
  • sustainability
  • production systems

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Published Papers (7 papers)

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Research

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14 pages, 3607 KiB  
Article
Comparison of MicroRNA Profiles in Extracellular Vesicles from Small and Large Goat Follicular Fluid
by Qiang Ding, Miaohan Jin, Peter Kalds, Chunhua Meng, Huili Wang, Jifeng Zhong, Xiaolong Wang and Yulin Chen
Animals 2021, 11(11), 3190; https://doi.org/10.3390/ani11113190 - 8 Nov 2021
Cited by 9 | Viewed by 2121
Abstract
Extracellular vesicles (EVs), which exist in the follicular fluid of ruminant ovaries, are considered as cargo carriers for the transfer of biomolecules to recipient cells. However, the functions and changes in EVs in antral follicles remain ambiguous. In the present study, we isolated [...] Read more.
Extracellular vesicles (EVs), which exist in the follicular fluid of ruminant ovaries, are considered as cargo carriers for the transfer of biomolecules to recipient cells. However, the functions and changes in EVs in antral follicles remain ambiguous. In the present study, we isolated and characterized EVs from goat follicular fluid by means of differential ultracentrifugation and Western blotting of marker proteins. Bioinformatics tools were used to detect miRNA expression levels in EVs. Different miRNA expression patterns of EVs exist in small to large follicles. Thirteen differentially expressed miRNAs (seven upregulated and six downregulated) were identified and used for analysis. A total of 1948 predicted target genes of 13 miRNAs were mapped to signaling pathways, and three significantly enriched pathways (FoxO, MAPK, and PI3K-AKT signaling pathways) were involved in follicular development, as revealed by KEGG enrichment analysis. Our findings suggest that EVs in follicular fluid play biofunctional roles during follicular development in goats. Full article
(This article belongs to the Special Issue Reproductive Physiology of Small Ruminants)
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9 pages, 595 KiB  
Article
Kisspeptin Stimulates the Pulsatile Secretion of Luteinizing Hormone (LH) during Postpartum Anestrus in Ewes Undergoing Continuous and Restricted Suckling
by José Manuel Hernández-Hernández, Graeme B. Martin, Carlos Miguel Becerril-Pérez, Arturo Pro-Martínez, César Cortez-Romero and Jaime Gallegos-Sánchez
Animals 2021, 11(9), 2656; https://doi.org/10.3390/ani11092656 - 9 Sep 2021
Cited by 7 | Viewed by 2649
Abstract
This study tested whether the intravenous application of kisspeptin can stimulate the pulsatile secretion of LH in suckling ewes during postpartum anestrus. Ten days after lambing, Pelibuey ewes were allocated among two groups: (1) continuous suckling (n = 8), where the lambs [...] Read more.
This study tested whether the intravenous application of kisspeptin can stimulate the pulsatile secretion of LH in suckling ewes during postpartum anestrus. Ten days after lambing, Pelibuey ewes were allocated among two groups: (1) continuous suckling (n = 8), where the lambs remained with their mothers; and (2) restricted suckling (n = 8), where the mothers suckled their lambs twice daily for 30 min. On Day 19 postpartum, the ewes were individually penned with ad libitum access to water and feed and given an indwelling catheter in each jugular vein. On Day 20, 4 mL of blood was sampled every 15 min from 08:00 to 20:00 h to determine LH pulse frequency. At 14:00 h, four ewes in each group received 120 μg of kisspeptin diluted in 3 mL of saline as a continuous infusion for 6 h; the remaining four ewes in each group received only saline. The interaction between kisspeptin and suckling type did not affect LH pulse frequency (p > 0.05). Before kisspeptin administration, pulse frequency was similar in all groups (1.50 ± 0.40 pulses per 6 h; p > 0.05). With the application of kisspeptin, pulse frequency increased to 3.50 ± 0.43 pulses per 6 h (p ≤ 0.014), so the concentration of LH (1.11 ± 0.14 ng mL−1) was greater in kisspeptin-treated ewes than in saline-treated ewes (0.724 ± 0.07 ng mL−1; p ≤ 0.040). The frequency of LH pulses was greater with restricted suckling than with continuous suckling (2.44 ± 0.29 versus 1.69 ± 0.29 pulses per 6 h; p ≤ 0.040). We conclude that intravenous application of kisspeptin increases the pulsatile secretion of LH in suckling ewes and that suckling might reduce kisspeptin neuronal activity, perhaps explaining the suppression of ovulation. Moreover, the effects of kisspeptin and suckling on pulsatile LH secretion appear to be independent, perhaps operating through different neural pathways. Full article
(This article belongs to the Special Issue Reproductive Physiology of Small Ruminants)
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13 pages, 856 KiB  
Article
Effects of Three Semen Extenders, Breeding Season Month and Freezing–Thawing Cycle on Spermatozoa Preservation of Portuguese Merino Sheep
by Margarida Fernandes, Pablo Rodríguez Hernández, João Simões and João Pedro Barbas
Animals 2021, 11(9), 2619; https://doi.org/10.3390/ani11092619 - 7 Sep 2021
Cited by 9 | Viewed by 3887
Abstract
This study aimed to evaluate and compare the effect of three semen extenders (S-EXT) on 22 spermatozoa (SPZ) parameters (subjective and computer-assisted sperm analysis evaluations), before and after semen cryopreservation throughout different months of the breeding season in the Portuguese Merino breed. According [...] Read more.
This study aimed to evaluate and compare the effect of three semen extenders (S-EXT) on 22 spermatozoa (SPZ) parameters (subjective and computer-assisted sperm analysis evaluations), before and after semen cryopreservation throughout different months of the breeding season in the Portuguese Merino breed. According to the multivariable model, the SPZ viability (alive %), kinetics subjective individual motility, total motility, total progressive motility and its subpopulations, and beat cross frequency) were higher in the egg yolk-based S-EXT improved by Estação Zootécnica National (Portugal) than in Ovixcell® or Andromed® extenders. All the differences were only observed in thawed semen, except for total motility and total progressive motility, in which Ovixcell® also showed the poorest results on fresh semen. An interaction effect between S-EXT and semen processing was observed on 72.3% (17/22) of the evaluated parameters, evidencing a variable cryoprotective action between S-EXT. The SPZ viability was poorer in the onset of the breeding season (end of April/early May) than in the previous middle breeding season (November/early December), suggesting the influence of a short anoestrous season on ejaculate quality, even though the volume and SPZ concentration of the ejaculates remained stable throughout the experiment. Additionally, S-EXT x semen processing x month interaction effect on 59.1% (13/22) of the evaluated parameters evidenced the importance of SPZ time collection in a natural environment to cryopreserve ram’s semen. We concluded that, overall, the egg yolk-based S-EXT provided a greater value to the cryopreservation of Merino rams´ semen. Nevertheless, the causes of the interaction effect between S-EXT, semen processing and/or month on several SPZ parameters should be addressed, including SPZ molecular research in new studies, in order to improve egg yolk-based as well as in egg yolk-free-based S-EXT. Full article
(This article belongs to the Special Issue Reproductive Physiology of Small Ruminants)
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11 pages, 263 KiB  
Article
Effect of Body Condition Score, Treatment Period and Month of the Previous Lambing on the Reproductive Resumption of Melatonin-Treated Sarda Breed Sheep during Spring
by Luisa Pulinas, Giovanni Cosso, Maria Consuelo Mura, Melissa Carvajal-Serna, Hatem Ouled Ahmed, Vincenzo Carcangiu and Sebastiano Luridiana
Animals 2021, 11(7), 1898; https://doi.org/10.3390/ani11071898 - 25 Jun 2021
Cited by 1 | Viewed by 1757
Abstract
Stakeholders place great emphasis upon rationalizing the management and rearing techniques which are utilized within sheep farms. The present study aimed to investigate factors which may improve the reproductive performance of melatonin-treated Sardinian sheep via a series of three trials. The first trial [...] Read more.
Stakeholders place great emphasis upon rationalizing the management and rearing techniques which are utilized within sheep farms. The present study aimed to investigate factors which may improve the reproductive performance of melatonin-treated Sardinian sheep via a series of three trials. The first trial (n = 100) investigated the effect of melatonin treatment alongside body condition score (BCS), the second trial (n = 150) investigated the effect of treatment alongside the date of treatment (treatment period) and the third trial (n = 150) investigated the effect of treatment alongside the previous lambing of the ewes. The findings indicated that melatonin is an effective tool for anticipating and improving the reproductive activity of in Sarda breed sheep during the springtime. Furthermore, to obtain optional results, melatonin implantation should be conducted in April, in ewes that have a BCS of >2.5 and that have passed their third month of lactation. Full article
(This article belongs to the Special Issue Reproductive Physiology of Small Ruminants)
16 pages, 1204 KiB  
Article
Sperm Behavior and Response to Melatonin under Capacitating Conditions in Three Sheep Breeds Subject to the Equatorial Photoperiod
by Melissa Carvajal-Serna, Jaime Antonio Cardozo-Cerquera, Henry Alberto Grajales-Lombana, Adriana Casao and Rosaura Pérez-Pe
Animals 2021, 11(6), 1828; https://doi.org/10.3390/ani11061828 - 18 Jun 2021
Cited by 3 | Viewed by 2136
Abstract
In this study, we demonstrated that, in seasonal Mediterranean ovine breeds, supplementing the TALP medium with cAMP-elevating agents (the cocktail medium) is effective for achieving ram sperm capacitation, and that melatonin is able to regulate this phenomenon. We investigated the behavior under capacitating [...] Read more.
In this study, we demonstrated that, in seasonal Mediterranean ovine breeds, supplementing the TALP medium with cAMP-elevating agents (the cocktail medium) is effective for achieving ram sperm capacitation, and that melatonin is able to regulate this phenomenon. We investigated the behavior under capacitating conditions using the TALP and cocktail mediums, and the response to melatonin, of spermatozoa from three sheep breeds (Colombian Creole, Romney Marsh, and Hampshire) subject to the equatorial photoperiod, during the dry and the rainy seasons. The cocktail medium was able to induce sperm capacitation, assayed by chlortetracycline staining and phosphotyrosine levels, to a greater extent than TALP, without a higher loss of viability (membrane integrity and viable spermatozoa without phosphatidylserine (PS) translocation). The addition of melatonin at 100 pM or 1 µM in the cocktail medium partially prevented the decrease in viability without PS translocation and the increase in capacitated spermatozoa from all breeds, with no significant effect on phosphotyrosine levels. Differences between breeds and seasons were evidenced. This study shows that melatonin is able to exert direct effects on spermatozoa in ovine breeds under equatorial photoperiod conditions, as it does in seasonal breeds located in temperate regions. Full article
(This article belongs to the Special Issue Reproductive Physiology of Small Ruminants)
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11 pages, 1406 KiB  
Article
Influence of Season and Liquid Storage at 16 °C on Beni Arouss Bucks’ Semen Quality
by Sara El Kadili, Nathalie Kirschvink, Marianne Raes, Jean Loup Bister, Bouchaib Archa, Ahmed Douaik and Mouad Chentouf
Animals 2020, 10(11), 1986; https://doi.org/10.3390/ani10111986 - 29 Oct 2020
Cited by 2 | Viewed by 2247
Abstract
The study aimed at determining the effect of storage and season on fresh semen of Beni Arouss goats. Ejaculates were collected at monthly intervals from seven mature bucks and were extended at a final concentration of 800 × 106 spermatozoa. ml-1 [...] Read more.
The study aimed at determining the effect of storage and season on fresh semen of Beni Arouss goats. Ejaculates were collected at monthly intervals from seven mature bucks and were extended at a final concentration of 800 × 106 spermatozoa. ml-1 and stored at 16 °C for 24 h. Semen motility, viability and normal morphology were assessed at 0, 4, 8 and 24 h after collection. Motility and normal morphology parameters were recorded using computer-assisted sperm analysis (CASA) and viability was analyzed using eosin–nigrosin staining. As expected, motility, viability and normal morphology parameters showed a significant reduction within 24 h of storage and during all seasons (p < 0.05). However, semen collected in summer maintained a better quality after 24 h of storage at 16 °C than semen collected during the other periods (p < 0.05). In conclusion, the storage ability of Beni Arouss bucks’ semen stored at 16 °C was significantly higher during the summer. Full article
(This article belongs to the Special Issue Reproductive Physiology of Small Ruminants)
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Review

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12 pages, 716 KiB  
Review
Reproductive Management of Rams and Ram Lambs during the Pre-Breeding Season in US Sheep Farms
by Martin G. Maquivar, Sarah M. Smith and Jan R. Busboom
Animals 2021, 11(9), 2503; https://doi.org/10.3390/ani11092503 - 26 Aug 2021
Cited by 26 | Viewed by 5613
Abstract
In sheep farms, achieving economic and productive efficiency and sustainability goals is directly related with the reproductive management of the animals. Genetically, the male contribution to the offspring is 50%, but in practical terms, there is a greater potential impact of the ram [...] Read more.
In sheep farms, achieving economic and productive efficiency and sustainability goals is directly related with the reproductive management of the animals. Genetically, the male contribution to the offspring is 50%, but in practical terms, there is a greater potential impact of the ram on reproductive results, because one male has the potential to affect a large group of females and therefore greatly affects the entire flock. Unfortunately, the selection of males for breeding in the US sheep industry has been traditionally conducted based on phenotypical traits, without a genetic/reproductive evaluation, and/or health assessment or records. Therefore, it is important to establish integrative management practices to select the rams and ram lambs to be used in the breeding season. Among these practices are genomic testing, a comprehensive breeding soundness evaluation and assessment of health status and records of the males before the breeding season, to detect and correct potential issues. Full article
(This article belongs to the Special Issue Reproductive Physiology of Small Ruminants)
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