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Biosensors
Special Issues
DNA Based Biosensors and Related Applications
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DNA Based Biosensors and Related Applications

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor Materials".

Deadline for manuscript submissions: closed (30 June 2022) | Viewed by 9473

Special Issue Editors

Dr. Giulia Selvolini

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Guest Editor
Department of Chemistry "Ugo Schiff", University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, FI, Italy
Interests: electrochemical biosensors; optical biosensors; DNA receptors; aptamer assays; food contaminants; conducting polymers; metal nanoparticles; voltammetric techniques
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Giovanna Marrazza

E-Mail Website
Guest Editor
Department of Chemistry “Ugo Schiff”, University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, Italy
Interests: immobilization procedure of biomolecules; protein–DNA complexes; aptamer; enzymatic sensors; thick-film technology; nanodispensing technologies; micro-flow systems; carbon nanotubes; nanoparticles; nanocomposite polymers; molecular imprinted polymers; protein-polymer conjugates
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Recent progresses in bioanalytical applications have significantly benefited from the synthesis and characterization of new classes of biomimetic receptors, as those developed by using inspiration from nature, and their huge potential as receptor elements in biosensing.

The potential of DNA-based nanostructures in sensing applications is derived from the interaction they can establish with proteins, nanoparticles, nucleotidic strands and ions, among other molecules. In this context, synthetic DNA sequences like aptamers show themselves as the ideal candidates as receptors, offering several advantages, such as no need of animal immunization, high chemical and thermal stability in quite extreme conditions, low cross reactivity and prolonged shelf life. Moreover, aptamers are easy to be immobilized and/or labelled by adding different functional groups to the nucleotidic sequence. Due to those features, these ligands have been used in diagnosis, therapeutics, target validation, drug industry, and as detection agents.

For this Special Issue, I invite you to share new developments in the growing field of DNA-based biosensors, in the form of original research or review articles, with a focus on their specific applications. The state of the art should be presented, as well as the addressing of the new challenges that are being faced and the understanding of the perspectives on the future of the field. I look forward to your contributions.

Dr. Giulia Selvolini
Prof. Dr. Giovanna Marrazza
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biosensors
  • DNA
  • aptamers
  • nucleotides
  • bioassays
  • nanomaterials
  • real-time analysis
  • sensor performance
  • sensing mechanism
  • point-of-care diagnostics
  • miniaturized systems
  • applications for health, environment, food and cultural heritage

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Published Papers (3 papers)

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11 pages, 3199 KiB  
Article
Facile Label-Free Electrochemical DNA Biosensor for Detection of Osteosarcoma-Related Survivin Gene
by Yao Chen, Yu Zhong, Ji-Xing Ye, Yun Lei and Ai-Lin Liu
Biosensors 2022, 12(9), 747; https://doi.org/10.3390/bios12090747 - 9 Sep 2022
Cited by 5 | Viewed by 2083
Abstract
A sensitive and selective electrochemical deoxyribonucleic acid (DNA) biosensor was developed for the determination of a osteosarcoma-related survivin gene by using celestine blue (CB) as a label-free hybridization indicator. The proposed strategy adopted a facile and low-cost working electrode with no need for [...] Read more.
A sensitive and selective electrochemical deoxyribonucleic acid (DNA) biosensor was developed for the determination of a osteosarcoma-related survivin gene by using celestine blue (CB) as a label-free hybridization indicator. The proposed strategy adopted a facile and low-cost working electrode with no need for other substances for electrode or DNA functionalization. The interaction mode between CB and DNA was studied by electrochemical and spectroscopic approaches, illustrating that the possible mode was intercalation with a binding number of 2 and a binding constant β of 1012.87. Moreover, the label-free electrochemical DNA biosensor exhibited a good linear relationship toward the target gene in a range from 1.00 nM to 50.00 nM with a detection limit as low as 0.046 nM using 3σ estimating system. This facile and low-cost electrochemical method realized the rapid detection and accurate quantification of the target sequence in complicated serum samples, endowing its promising potential in the diagnosis and monitoring of genetic diseases. Full article
(This article belongs to the Special Issue DNA Based Biosensors and Related Applications)
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13 pages, 2659 KiB  
Article
APTES-Modified Remote Self-Assembled DNA-Based Electrochemical Biosensor for Human Papillomavirus DNA Detection
by Yuxing Yang, Yang Qing, Xudong Hao, Chenxin Fang, Ping Ouyang, Haiyu Li, Zhencui Wang, Yazhen Liao, Haobin Fang and Jie Du
Biosensors 2022, 12(7), 449; https://doi.org/10.3390/bios12070449 - 24 Jun 2022
Cited by 7 | Viewed by 3113
Abstract
High-risk human papillomavirus (HPV) infection is an important cause of cervical cancer formation; therefore, being able to detect high-risk HPV (e.g., HPV-16) is important for the early treatment and prevention of cervical cancer. In this study, a combination of a 3-aminopropyltriethoxysilane (APTES) modified [...] Read more.
High-risk human papillomavirus (HPV) infection is an important cause of cervical cancer formation; therefore, being able to detect high-risk HPV (e.g., HPV-16) is important for the early treatment and prevention of cervical cancer. In this study, a combination of a 3-aminopropyltriethoxysilane (APTES) modified gold electrode and a super sandwich structure was creatively developed, resulting in the development of a biosensor that is both sensitive and stable for the detection of HPV-16. The electrochemical biosensor possesses a lower detection limit compared with previous studies with an LOD of 5.475 × 10−16 mol/L and it possesses a wide linear range from 1.0 × 10−13 mol/L to 1.0 × 10−6 mol/L (R2 = 0.9923) for the target DNA. The experimental data show that the sensor has good stability, and there is no significant decrease in the current response value after 7 days in the low-temperature environment. In addition, the sensor proved to be a powerful clinical tool for disease diagnosis because it showed good interference resistance in complex human serum samples. Full article
(This article belongs to the Special Issue DNA Based Biosensors and Related Applications)
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24 pages, 5861 KiB  
Article
Label-Free Electrochemical Aptasensor for the Detection of the 3-O-C12-HSL Quorum-Sensing Molecule in Pseudomonas aeruginosa
by Denisa Capatina, Teodora Lupoi, Bogdan Feier, Adrian Blidar, Oana Hosu, Mihaela Tertis, Diana Olah, Cecilia Cristea and Radu Oprean
Biosensors 2022, 12(7), 440; https://doi.org/10.3390/bios12070440 - 22 Jun 2022
Cited by 21 | Viewed by 3319
Abstract
Pseudomonas aeruginosa, an opportunistic Gram-negative bacterium, is one of the main sources of infections in healthcare environments, making its detection very important. N-3-oxo-dodecanoyl L-homoserine lactone (3-O-C12-HSL) is a characteristic molecule of quorum sensing—a form of cell-to-cell communication between bacteria—in P. [...] Read more.
Pseudomonas aeruginosa, an opportunistic Gram-negative bacterium, is one of the main sources of infections in healthcare environments, making its detection very important. N-3-oxo-dodecanoyl L-homoserine lactone (3-O-C12-HSL) is a characteristic molecule of quorum sensing—a form of cell-to-cell communication between bacteria—in P. aeruginosa. Its detection can allow the determination of the bacterial population. In this study, the development of the first electrochemical aptasensor for the detection of 3-O-C12-HSL is reported. A carbon-based screen-printed electrode modified with gold nanoparticles proved to be the best platform for the aptasensor. Each step in the fabrication of the aptasensor (i.e., gold nanoparticles’ deposition, aptamer immobilization, incubation with the analyte) was optimized and characterized using cyclic voltammetry, differential pulse voltammetry, and electrochemical impedance spectroscopy. Different redox probes in solution were evaluated, the best results being obtained in the presence of [Fe(CN)6]4−/[Fe(CN)6]3−. The binding affinity of 106.7 nM for the immobilized thiol-terminated aptamer was determined using surface plasmon resonance. The quantification of 3-O-C12-HSL was performed by using the electrochemical signal of the redox probe before and after incubation with the analyte. The aptasensor exhibited a logarithmic range from 0.5 to 30 µM, with a limit of detection of 145 ng mL−1 (0.5 µM). The aptasensor was successfully applied for the analysis of real samples (e.g., spiked urine samples, spiked microbiological growth media, and microbiological cultures). Full article
(This article belongs to the Special Issue DNA Based Biosensors and Related Applications)
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