Saccharomyces cerevisiae Strains and Fermentation: 2nd Edition

A special issue of Fermentation (ISSN 2311-5637). This special issue belongs to the section "Microbial Metabolism, Physiology & Genetics".

Deadline for manuscript submissions: 31 December 2024 | Viewed by 3824

Special Issue Editor


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Guest Editor
Department of Agronomy Food Natural Resources Animals and Environment (DAFNAE), University of Padova, Legnaro, Italy
Interests: wine; fermentation; oenology; wine yeasts; yeasts selection; wine microbiology; non-conventional yeasts; food science

Special Issue Information

Dear Colleagues,

The importance of alcoholic fermentation by yeasts and malolactic fermentation by lactic acid bacteria goes far beyond ethanol production and acid management. In fact, these processes, and thereby the native and artificial microflora, influence the entire production chain and the final composition and quality of the produced wine. The decoding of microbial metabolic pathways but also, for example, the derivation of parameters for process control and monitoring are current fields of research.

The progressing climate change is a huge challenge to which one can only respond adequately with intelligent and modern concepts and solutions in vineyards and wineries. These concepts often include the utilization of a wide variety of yeast and bacterial species, that can only be applied securely by means of intelligent process control. Digitalization also plays an important role at this point. Rediscovered traditional knowledge and experience has often only been made useable and profitable again through modern research. This is especially valid for wine production, where consumers increasingly prefer unique and, above all, authentic products.

This Special Issue is intended to provide an opportunity to publish recent studies that address current matters of wine fermentation. If you would like to contribute a review paper, please contact one of the editors to discuss the topic’s relevance before submitting the manuscript.

Dr. Chiara Nadai
Guest Editor

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Keywords

  • Saccharomyces cerevisiae strains
  • fermentation
  • wine
  • oenology
  • microflora
  • malolactic fermentation
  • metabolic pathways
  • climate change

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Published Papers (3 papers)

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Research

14 pages, 1236 KiB  
Article
Diversity of Saccharomyces cerevisiae Yeast Strains in Granxa D’Outeiro Winery (DOP Ribeiro, NW Spain): Oenological Potential
by Pilar Blanco, Estefanía García-Luque, Rebeca González, Elvira Soto, José Manuel M. Juste and Rafael Cao
Fermentation 2024, 10(9), 475; https://doi.org/10.3390/fermentation10090475 - 13 Sep 2024
Viewed by 745
Abstract
Yeasts play an essential role in the aroma and sensory profiles of wines. Spontaneous fermentations were carried out at the newly built winery of Granxa D’Outeiro. Yeasts were isolated from must at different stages of fermentation. Colonies belonging to Saccharomyces cerevisiae were characterised [...] Read more.
Yeasts play an essential role in the aroma and sensory profiles of wines. Spontaneous fermentations were carried out at the newly built winery of Granxa D’Outeiro. Yeasts were isolated from must at different stages of fermentation. Colonies belonging to Saccharomyces cerevisiae were characterised at the strain level by mtDNA-RFLPs. General chemical parameters and aroma profiles of the wines were determined using official OIV methodology and GC-MS analysis, respectively. The diversity of S. cerevisiae per fermentation ranged from 5 to 13 different strains depending on the grapevine variety. Out of 24 strains, strain B was the dominant yeast in most fermentations at different proportions, but strains D, E, and H also reached up to 25% of the total population in some fermentations. The yeast diversity was higher in the Lado fermentation than in those containing Treixadura. The chemical compositions of the wines revealed differences among them, with Loureira and Albariño wines showing the highest content of volatile compounds. The evaluation of their technological properties revealed the oenological potential of some strains of S. cerevisiae. The strains showing the best scores were selected to be used in future vintages to enhance the typicality of wines in the Granxa D’Outeiro winery. Full article
(This article belongs to the Special Issue Saccharomyces cerevisiae Strains and Fermentation: 2nd Edition)
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17 pages, 2222 KiB  
Article
Phenotypic Characterization of Fermentation Performance and Stress Tolerance in Commercial Ale Yeast Strains
by Anqi Chen, Qiqi Si, Qingyun Xu, Chenwei Pan, Yuhan Cheng and Jian Chen
Fermentation 2024, 10(7), 364; https://doi.org/10.3390/fermentation10070364 - 18 Jul 2024
Viewed by 1595
Abstract
Yeast plays a crucial role in the fermentation industry, particularly in alcoholic beverage production, where robustness and metabolic flexibility are essential. This study aimed to investigate the stress tolerance and metabolic capabilities of seven commercial ale yeast strains under various stress conditions, including [...] Read more.
Yeast plays a crucial role in the fermentation industry, particularly in alcoholic beverage production, where robustness and metabolic flexibility are essential. This study aimed to investigate the stress tolerance and metabolic capabilities of seven commercial ale yeast strains under various stress conditions, including temperature, pH, osmotic pressure, glucose starvation, and ethanol concentration. Detailed growth assays and stress tolerance tests were utilized to evaluate fermentation efficiency, carbon source utilization, and stress adaptation. Significant variability was observed among the strains. ACY169 and ACY150 demonstrated high overall stress tolerance, making them suitable for high-gravity brewing and processes involving extreme temperature fluctuations. ACY10 showed robust performance under acid stress, making it ideal for sour beer production. In contrast, ACY5 exhibited limited adaptability under stress, with longer doubling times and reduced metabolic activity. The study also revealed differences in carbon source utilization, with ACY169 displaying exceptional metabolic versatility by efficiently fermenting various sugars, including glucose, fructose, maltose, and raffinose. ACY10 and ACY150 exhibited balanced fermentation profiles with high ethanol production rates, while ACY9 demonstrated the highest glucose consumption rate but lower ethanol yields and significant acidification. Full article
(This article belongs to the Special Issue Saccharomyces cerevisiae Strains and Fermentation: 2nd Edition)
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15 pages, 2369 KiB  
Article
Bioprospecting and Selection of Indigenous Saccharomyces cerevisiae Yeasts from Pozo de Los Algarrobos (Argentina) for the Production of Genuine White Wines
by Diego Bernardo Petrignani, María Victoria Mestre, Mercedes Fabiana Vargas, Selva Valeria Chimeno, Fabio Vazquez and Yolanda Paola Maturano
Fermentation 2024, 10(6), 279; https://doi.org/10.3390/fermentation10060279 - 25 May 2024
Viewed by 1018
Abstract
Yeasts play a crucial role in the winemaking process contributing to the typicity and originality of wines in a region. Therefore, the aim of the present study was to isolate, characterize, and select yeasts from the Geographical Indication “Pozo de Los Algarrobos”, San [...] Read more.
Yeasts play a crucial role in the winemaking process contributing to the typicity and originality of wines in a region. Therefore, the aim of the present study was to isolate, characterize, and select yeasts from the Geographical Indication “Pozo de Los Algarrobos”, San Juan, Argentina. Yeasts were directly isolated from grapes and at different stages of spontaneous fermentations of Vitis vinifera Viognier and Chardonnay varieties. Molecular and intraspecific identification of Saccharomyces cerevisiae yeasts was conducted using the D1/D2 domain and interdelta, respectively, observing 13 different yeast strains from Viognier and 12 from Chardonnay vinifications. Based on the enological traits assayed, two strains, V22 (Viognier) and C14 (Chardonnay), were selected for further studies. Microvinifications with these yeasts were carried out with Viognier and Chardonnay grape must in 2 L flasks, and the resulting wines were analytically and sensorially evaluated. Overall, strain V22 produced wines with positive and particular sensory properties, associated with fruity and floral aromas, color intensity, sweetness, aromatic persistence, and varietal typicity. Consequently, biomass propagation of V22 was conducted to inoculate pilot- (100 L) and industrial (12,000 L)-scale fermentations. V22 resulted in a correct wine fermentation performance obtaining a final product with distinctive and genuine properties. Full article
(This article belongs to the Special Issue Saccharomyces cerevisiae Strains and Fermentation: 2nd Edition)
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