Application of Isothermal Nucleic Acid Amplification for Rapid Foodborne Pathogen Detection: Current Status and Future Prospects

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Microbiology".

Deadline for manuscript submissions: closed (28 October 2024) | Viewed by 1652

Special Issue Editor


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Guest Editor
Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, China
Interests: isothermal nucleic acid amplification and biosensors; synthetic biology (CRISPR-Cas) and molecular diagnostics; rapid detection systems and all-in-one microfluidic diagnostics

Special Issue Information

Dear Colleagues,

Rapid foodborne pathogen detection is of importance to human health and public health, especially for the early prevention and control of foodborne pathogen infections. One pioneering technique among the molecular biological methods that can be used to achieve rapid foodborne pathogen detection is isothermal nucleic acid amplification (INAA). As of now, a variety of INAA methods have been reported, such as loop-mediated isothermal amplification (LAMP), recombinase polymerase amplification (RPA), cross-priming amplification (CPA), and isothermal multiple-self-matching-initiated amplification (IMSA). In addition to the commonly used tube-based detection format, INAA has been coupled with microfluidic chips, portable devices, new nanomaterials, and so on, aiming to achieve “sample-in-answer-out” analysis, digital quantification, and onsite rapid visual detection. Further, the coupling of INAA with CRISPR/Cas systems and Argonaute nucleases greatly advances the detection in terms of precision and accuracy. As such, we are establishing a Special Issue, entitled “Application of Isothermal Nucleic Acid Amplification for Rapid Foodborne Pathogen Detection: Current Status and Future Prospects”, in the hopes of receiving and publishing methodological papers and reviews in the realm of rapid foodborne pathogen detection.

Prof. Dr. Xiong Ding
Guest Editor

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Keywords

  • foodborne pathogens
  • isothermal nucleic acid amplification
  • CRISPR/Cas system
  • argonaute nuclease
  • microfluidics and microfluidic chip
  • digital detection
  • sample-in–answer-out system
  • rapid detection
  • onsite detection
  • visual detection

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Published Papers (1 paper)

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Research

10 pages, 1745 KiB  
Article
High-Performance Detection of Mycobacterium bovis in Milk Using Recombinase-Aided Amplification–Clustered Regularly Interspaced Short Palindromic Repeat–Cas13a–Lateral Flow Detection
by Jieru Wang, Nan Wang, Lei Xu, Xiaoyu Zeng, Junsheng Cheng, Xiaoqian Zhang, Yinghui Zhang, Dongdong Yin, Jiaojiao Gou, Xiaocheng Pan and Xiaojie Zhu
Foods 2024, 13(11), 1601; https://doi.org/10.3390/foods13111601 - 21 May 2024
Viewed by 1194
Abstract
Mycobacterium bovis (M. bovis), the microorganism responsible for bovine tuberculosis (bTB), is transferred to people by the ingestion of unpasteurized milk and unprocessed fermented milk products obtained from animals with the infection. The identification of M. bovis in milk samples is [...] Read more.
Mycobacterium bovis (M. bovis), the microorganism responsible for bovine tuberculosis (bTB), is transferred to people by the ingestion of unpasteurized milk and unprocessed fermented milk products obtained from animals with the infection. The identification of M. bovis in milk samples is of the utmost importance to successfully prevent zoonotic diseases and maintain food safety. This study presents a comprehensive description of a highly efficient molecular test utilizing recombinase-aided amplification (RPA)–clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein (Cas) 13a–lateral flow detection (LFD) for M. bovis detection. In contrast to ELISA, RPA–CRISPR–Cas13a–LFD exhibited greater accuracy and sensitivity in the detection of M. bovis in milk, presenting a detection limit of 2 × 100 copies/μL within a 2 h time frame. The two tests exhibited a moderate level of agreement, as shown by a kappa value of 0.452 (95%CI: 0.287–0.617, p < 0.001). RPA–CRISPR–Cas13a–LFD holds significant potential as a robust platform for pathogen detection in complex samples, thereby enabling the more dependable regulation of food safety examination, epidemiology research, and medical diagnosis. Full article
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