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Molecular Breeding of Plants: From CRISPR/Cas9 to Transcriptomics and MABc

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Plant Sciences".

Deadline for manuscript submissions: 24 April 2025 | Viewed by 1613

Special Issue Editor


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Guest Editor
Division of Horticultural Biotechnology, Hankyung National University, Anseong 17579, Republic of Korea
Interests: functional analysis of genes via CRISPR/Cas9; functional genomics; MABc (marker-assisted backcross); transcriptomics; plant biotechnology; molecular breeding in plants
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Special Issue Information

Dear Colleagues,

The study of cellular processes using novel genome-reading strategies, especially CRISPR/Cas9, is becoming increasingly feasible and powerful. Clustered regular interspersed short palindromic repeat (CRISPR) RNA (crRNA) is commonly used with the CRISPR-associated 9 (Cas9) nuclease to disrupt genes for loss-of-function assays. These mechanisms are approaches to introduce or reverse DNA fragments exhibiting polymorphisms in the genome using the error-prone nonhomologous end-joining (NHEJ) DNA repair pathway or the much less efficient homology directed repair (HDR) pathway. This report is interesting to see how gene editing, such as NHEJ and HDR, affects the expression of many other genes in the genome, as well as breeding mutations. Therefore, as a means to secure various genetic resources, we plan to accept papers presented on the development of new breeding programs using gene editing technology and various analysis methods. This Special Issue of the International Journal of Molecular Sciences (IJMS) welcomes articles on reviews and findings.

Prof. Dr. Kwon-Kyoo Kang
Guest Editor

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Keywords

  • CRISPR/Cas9
  • transcriptomics
  • marker-assisted breeding
  • genome editing
  • plant genetics
  • gene expression
  • plant genomics
  • quantitative trait loci (QTL)
  • crop improvement

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Published Papers (1 paper)

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Research

12 pages, 3044 KiB  
Article
Physicochemical Properties and Antioxidant Activity of CRISPR/Cas9-Edited Tomato SGR1 Knockout (KO) Line
by Jin Young Kim, Dong Hyun Kim, Me-Sun Kim, Yu Jin Jung and Kwon Kyoo Kang
Int. J. Mol. Sci. 2024, 25(10), 5111; https://doi.org/10.3390/ijms25105111 - 8 May 2024
Viewed by 1126
Abstract
Tomatoes contain many secondary metabolites such as β-carotene, lycopene, phenols, flavonoids, and vitamin C, which are responsible for antioxidant activity. SlSGR1 encodes a STAY-GREEN protein that plays a critical role in the regulation of chlorophyll degradation in tomato leaves and fruits. Therefore, [...] Read more.
Tomatoes contain many secondary metabolites such as β-carotene, lycopene, phenols, flavonoids, and vitamin C, which are responsible for antioxidant activity. SlSGR1 encodes a STAY-GREEN protein that plays a critical role in the regulation of chlorophyll degradation in tomato leaves and fruits. Therefore, the present study was conducted to evaluate the sgr1 null lines based on their physicochemical characteristics, the content of secondary metabolites, and the γ-Aminobutyric acid (GABA) content. The total soluble solids (TSS), titrated acidity (TA), and brix acid ratio (BAR) of the sgr1 null lines were higher than those of the wild type(WT). Additionally, the sgr1 null lines accumulated higher levels of flavor-inducing ascorbic acid and total carotenoids compared to WT. Also, the total phenolic content, total flavonoids, GABA content, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical content of the sgr1 null lines were higher than those of the WT. Therefore, these studies suggest that the knockout of the SGR1 gene by the CRISPR/Cas9 system can improve various functional compounds in tomato fruit, thereby satisfying the antioxidant properties required by consumers. Full article
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