Life | Special Issue : Plant–Nematode Interaction

Editorial

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4 pages, 172 KiB

Open AccessEditorial

Research Advances in the Plant–Nematode Interaction

by Jia You, Yanfeng Hu and Jingsheng Chen

Life 2023, 13(8), 1722; https://doi.org/10.3390/life13081722 - 10 Aug 2023

Viewed by 1229

Abstract

Nematodes, which are highly important and abundant animals in the animal kingdom, demonstrate remarkable adaptability to various environments [...] Full article

(This article belongs to the Special Issue Plant–Nematode Interaction)

Research

Jump to: Editorial, Review

15 pages, 2551 KiB

Open AccessArticle

Root-Knot-Nematode-Encoded CEPs Increase Nitrogen Assimilation

by Shova Mishra, Weiming Hu and Peter DiGennaro

Life 2023, 13(10), 2020; https://doi.org/10.3390/life13102020 - 7 Oct 2023

Cited by 2 | Viewed by 1712

Abstract

C-terminally encoded peptides (CEPs) are plant developmental signals that regulate growth and adaptive responses to nitrogen stress conditions. These small signal peptides are common to all vascular plants, and intriguingly have been characterized in some plant parasitic nematodes. Here, we sought to discover [...] Read more.

C-terminally encoded peptides (CEPs) are plant developmental signals that regulate growth and adaptive responses to nitrogen stress conditions. These small signal peptides are common to all vascular plants, and intriguingly have been characterized in some plant parasitic nematodes. Here, we sought to discover the breadth of root-knot nematode (RKN)-encoded CEP-like peptides and define the potential roles of these signals in the plant–nematode interaction, focusing on peptide activity altering plant root phenotypes and nitrogen uptake and assimilation. A comprehensive bioinformatic screen identified 61 CEP-like sequences encoded within the genomes of six root-knot nematode (RKN; Meloidogyne spp.) species. Exogenous application of an RKN CEP-like peptide altered A. thaliana and M. truncatula root phenotypes including reduced lateral root number in M. truncatula and inhibited primary root length in A. thaliana. To define the role of RKN CEP-like peptides, we applied exogenous RKN CEP and demonstrated increases in plant nitrogen uptake through the upregulation of nitrate transporter gene expression in roots and increased 15N/14N in nematode-formed root galls. Further, we also identified enhanced nematode metabolic processes following CEP application. These results support a model of parasite-induced changes in host metabolism and inform endogenous pathways to regulate plant nitrogen assimilation. Full article

(This article belongs to the Special Issue Plant–Nematode Interaction)

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11 pages, 427 KiB

Open AccessArticle

Evaluation Soybean Cultivars for Reaction to Heterodera glycines Populations HG Types 7 and 1.3.4.7 in Northeast China

by Jingsheng Chen, Yuanyuan Zhou, Yanfeng Hu, Di Zhao, Changjun Zhou, Rujie Shi, Miao Sun, Li Zhang, Guowei Chen, Haiyan Li, Lijie Chen and Guosheng Xiao

Life 2023, 13(1), 248; https://doi.org/10.3390/life13010248 - 16 Jan 2023

Cited by 2 | Viewed by 2030

Abstract

Soybean cyst nematode Heterodera glycines (SCN) is a major threat to global soybean production. Effective management of this disease is dependent on the development of resistant cultivars. Two SCN HG Types, 7 and 1.3.4.7. were previously identified as prevalent H. glycines populations in [...] Read more.

Soybean cyst nematode Heterodera glycines (SCN) is a major threat to global soybean production. Effective management of this disease is dependent on the development of resistant cultivars. Two SCN HG Types, 7 and 1.3.4.7. were previously identified as prevalent H. glycines populations in Northeast China. In order to evaluate soybean cultivars resistant to local SCN populations, 110 domestic commercial soybeans from different regions of Northeast China were assessed in the greenhouse to determine their potential as novel sources of resistance. The results suggested that cultivars responded differently to the two HG types. Of the 110 soybean cultivars evaluated, 24 accessions were classified as resistant or moderately resistant to HG Type 7, and five cultivars were classified as resistant or moderately resistant to HG Type 1.3.4.7. Among the tested cultivars, Kangxian 12 and Qingdou 13 had resistance response to both HG types 7 and 1.3.4.7. Thus, these broad-based SCN cultivars will be the valuable materials in the SCN resistance breeding program. Full article

(This article belongs to the Special Issue Plant–Nematode Interaction)

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17 pages, 4623 KiB

Open AccessArticle

Tare Soil Disinfestation from Cyst Nematodes Using Inundation

by Beatrice Berger, Matthias Daub, Kathleen Gärtner, Matthias Becker and Stephan König

Life 2023, 13(1), 57; https://doi.org/10.3390/life13010057 - 24 Dec 2022

Cited by 3 | Viewed by 1916

Abstract

The dissemination of soil tares in the potato and sugar beet processing industry is one of the main paths for the spread of potato cyst nematodes (PCN), a severe quarantine pest. Efficient measures for the disinfestation of tare soil from PCN, but also [...] Read more.

The dissemination of soil tares in the potato and sugar beet processing industry is one of the main paths for the spread of potato cyst nematodes (PCN), a severe quarantine pest. Efficient measures for the disinfestation of tare soil from PCN, but also from beet cyst nematodes (BCN), are needed. In our study, Globodera pallida (a PCN) and Heterodera schachtii (a BCN) cysts were sealed in gauze bags and imbedded in sedimentation basins. The cysts were either placed (a) in a presedimentation basin (Brukner basin) for three days, (b) in the presedimentation basin for three days and subsequently in sedimentation basins for nine weeks or (c) in sedimentation basins for nine weeks (without presedimentation). We tested the viability of the eggs and juveniles by hatching assays and using the reproduction rates in bioassays. We demonstrated that PCN and BCN imbedded in a sedimentation basin were only still showing some hatching activity after 2.5 weeks, while no hatching was observed when an additional Brukner basin treatment was conducted before sedimentation. Full article

(This article belongs to the Special Issue Plant–Nematode Interaction)

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13 pages, 3510 KiB

Open AccessArticle

Resistance Evaluation for Native Potato Accessions against Late Blight Disease and Potato Cyst Nematodes by Molecular Markers and Phenotypic Screening in India

by Dalamu, Jagesh Kumar Tiwari, Aarti Bairwa, Nisha Bhatia, Rasna Zinta, Nimisha Kaushal, Vinod Kumar, Ashwani K. Sharma, Sanjeev Sharma, Babita Choudhary, Satish Kumar Luthra, Tanuja Buckseth, Rajesh K. Singh, Ajay K. Thakur, Manoj Kumar and Devendra Kumar

Life 2023, 13(1), 33; https://doi.org/10.3390/life13010033 - 23 Dec 2022

Cited by 8 | Viewed by 2606

Abstract

The potato originated in southern Peru and north-western Bolivia (South America). However, native accessions have also been cultivated in India for many years. Late blight, caused by the fungus Phytophthora infestans, is the most devastating potato disease, while potato cyst nematode ( [...] Read more.

The potato originated in southern Peru and north-western Bolivia (South America). However, native accessions have also been cultivated in India for many years. Late blight, caused by the fungus Phytophthora infestans, is the most devastating potato disease, while potato cyst nematode (Globodera spp.) (PCN) is another economically significant quarantine-requiring pest in India. In this study, we have generated a new Indian native collection of 94 potato accessions collected from different parts India. These accessions were screened against late blight and potato cyst nematode resistance by using gene-based molecular markers and phenotypic screening methods. Marker assisted selection using R1 gene-specific marker CosA₂₁₀ revealed a late blight resistance gene in 11 accessions. PCN resistance bands were found in 3 accessions with marker TG689₁₄₁, 5 accessions with marker 57R₄₅₂, and 1 accession having Gro1-4-1₆₀₂ marker for G. rostochiensis (Ro1,4), while 64 accessions amplified marker HC₂₇₆ indicating G. pallida (Pa2,3) resistance gene (GpaV_vrn QTL). On the other hand, phenotypic screening against late blight resistance under natural epiphytic conditions (hot-spot) revealed three accessions with high resistance, while others were resistant (1 accession), moderately resistant (5 accessions), susceptible (29 accessions), and highly susceptible (56 accessions). For G. rostochiensis (golden cyst nematode) and G. pallida (white cyst nematode) resistance, accessions were grouped into highly resistant (3, 3), resistant (0, 2), moderately resistant (6, 29), susceptible (32, 30), and highly susceptible (53, 30), respectively, for the two PCN species. Collectively, we identified promising accessions with high resistance to late blight (JG-1, Kanpuria Safed, and Rangpuria), and also highly resistant to both Globodera species (Garlentic, Jeevan Jyoti, and JG-1). Our findings suggested that these accessions would be useful for late blight and PCN resistance breeding, as well as future molecular studies in potatoes. Full article

(This article belongs to the Special Issue Plant–Nematode Interaction)

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18 pages, 3578 KiB

Open AccessArticle

Phytochemical Analysis and Binding Interaction of Cotton Seed Cake Derived Compounds with Target Protein of Meloidogyne incognita for Nematicidal Evaluation

by Fahad M. Almutairi, Amir Khan, Mohammad Rehan Ajmal, Rizwan Hasan Khan, Mohd Farhan Khan, Hira Lal, Mohammad Fahad Ullah, Faheem Ahmad, Lukman Ahamad, Arshad Khan, Hussain Arif and M. Ayaz Ahmad

Life 2022, 12(12), 2109; https://doi.org/10.3390/life12122109 - 15 Dec 2022

Cited by 10 | Viewed by 2795

Abstract

The root-knot nematode Meloidogyne incognita is one of the most damaging plant-parasitic nematodes and is responsible for significant crop losses worldwide. Rising human health and environmental concerns have led to the withdrawal of commonly used chemical nematicides. There has been a tremendous demand [...] Read more.

The root-knot nematode Meloidogyne incognita is one of the most damaging plant-parasitic nematodes and is responsible for significant crop losses worldwide. Rising human health and environmental concerns have led to the withdrawal of commonly used chemical nematicides. There has been a tremendous demand for eco-friendly bio-nematicides with beneficial properties to the nematode hosting plants, which encourages the need for alternative nematode management practices. The current study was undertaken to determine the nematicidal potential of cotton seed cake (CSC) against second-stage juvenile (J2) hatching, J2 mortality, and J2 penetration of M. incognita in tomato plants in vitro. J2s and egg masses of M. incognita were exposed to four concentrations (250, 500, 750, and 1000 mg/L) of CSC extracts. The higher J2 mortality and inhibition of J2 hatching were found at 1000 mg/L, while the least effective result was observed at 250 mg/L of the CSC extract. The CSC extract applied with the concentrations mentioned above also showed inhibition of J2 penetration in tomato roots; 1000 mg/L showed the highest inhibition of penetration, while 250 mg/L displayed the least inhibition. Using gas chromatography-mass spectroscopy, we identified 11 compounds, out of which 9,12-Octadecadienoic acid, Hexadecanoic acid, and Tetradecanoic acid were found as major compounds. Subsequently, in silico molecular docking was conducted to confirm the nematicidal behavior of CSC based on binding interactions of the above three major compounds with the targeted protein acetylcholine esterase (AChE) of M. incognita. The values of binding free energy are −5.3, −4.5, and −4.9 kcal/mol, observed for 9,12-Octadecadienoic acid, n-Hexadecanoic acid, and Tetradecanoic acid, respectively, suggesting that 9,12-Octadecadienoic acid binds with the receptor AChE more efficiently than the other two ligands. This study indicates that CSC has nematicidal potential that can be used to control M. incognita for sustainable agriculture. Full article

(This article belongs to the Special Issue Plant–Nematode Interaction)

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19 pages, 1261 KiB

Open AccessArticle

Can Agricultural Practices in Strawberry Fields Induce Plant–Nematode Interaction towards Meloidogyne-Suppressive Soils?

by Mostafa M. A. Hammam, Hassan Abd-El-Khair, Wafaa M. A. El-Nagdi and Mahfouz M. M. Abd-Elgawad

Life 2022, 12(10), 1572; https://doi.org/10.3390/life12101572 - 10 Oct 2022

Cited by 5 | Viewed by 1775

Abstract

The importance of benign approaches to manage the root-knot nematodes (RKNs, Meloidogyne spp.) in strawberry farms has become more evident with increasing strawberry production and export in Egypt. Therefore, data accumulated on biosolarization and soil amendments to favor beneficial microorganisms and maximize their [...] Read more.

The importance of benign approaches to manage the root-knot nematodes (RKNs, Meloidogyne spp.) in strawberry farms has become more evident with increasing strawberry production and export in Egypt. Therefore, data accumulated on biosolarization and soil amendments to favor beneficial microorganisms and maximize their impact on RKN management are built on a robust historical research foundation and should be exploited. We examined RKN population levels/parameters in three strawberry export governorates, six farms per governorate, to characterize the exact production practices that are responsible for RKN-suppressive soils. All selected farms enjoyed soil biodisinfestation resulting from incorporating organic amendments followed by a plastic cover to suppress soil pathogens. Various safe and inexpensive agricultural practices in the El-Ismailia and El-Beheira governorates were compared to the toxic and expensive fumigants that could eliminate RKNs in the Al-Qalyubia governorate. Two farms at El-Ismailia were of special interest as they ultimately showed almost zero counts of RKNs. The two farms were characterized by incorporating cow manure [containing 0.65% total nitrogen, 21.2 carbon to nitrogen (C/N) ratio] and poultry manure (0.72% total nitrogen, 20.1 C/N ratio) followed by soil solarization via transparent, 80-µm thick plastic covers for 60–65 summer days as pre-strawberry cultivation practices, and similar covers were used after transplanting. Typically, the longer the pre-plant soil solarization period with thicker transparent plastic covers, the better it could suppress the RKN population densities in the tested farms. Their soils were characterized by relatively high pH and low electrical conductivity. The significant development in biocontrol genera/species abundance and frequency could explain the lower (p < 0.0001) RKN population levels inhabiting the farms of El-Ismailia than the El-Beheira governorate. These factors could provide the first approximation of key practices and factors that could collectively contribute to distinguishing and exploiting soil suppressiveness against RKNs. We discussed edaphic properties and production practices that could modulate populations of natural RKN antagonists for sustainable strawberry cultivation. Full article

(This article belongs to the Special Issue Plant–Nematode Interaction)

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15 pages, 3431 KiB

Open AccessArticle

Soil Amendments with Spearmint, Peppermint and Rosemary Enhance the Community of Free-Living Nematodes and Improve Soil Quality, While Having Strikingly Different Effects on Plant Growth

by Maria D. Argyropoulou, Maria Karmezi, Maria Tsiafouli, Dimitris Chalkos, Areti Bountla and Despoina Vokou

Life 2022, 12(8), 1121; https://doi.org/10.3390/life12081121 - 26 Jul 2022

Cited by 5 | Viewed by 2252

Abstract

Sustainable farming practices aim to replace agrochemicals with plant-based alternatives to increase productivity and soil quality. To evaluate the potential use of aromatic plants as soil amendments in tomato seedbeds, in a greenhouse experiment, we used spearmint, peppermint, and rosemary, separately, as soil [...] Read more.

Sustainable farming practices aim to replace agrochemicals with plant-based alternatives to increase productivity and soil quality. To evaluate the potential use of aromatic plants as soil amendments in tomato seedbeds, in a greenhouse experiment, we used spearmint, peppermint, and rosemary, separately, as soil amendments, in pots sown with tomato, and studied their effect on seedling growth, soil nutrients, and the soil nematode community in terms of trophic and functional structure, metabolic footprint, and genera composition. Non-amended soil was used in the control pots. We further explored the dynamics of the plant–soil–nematode interactions by using aromatic plants at different stages of decomposition (0, 28, and 56 days). Incorporating aromatic plants into the soil led to the proliferation of free-living nematodes, especially of the opportunistic kind, resulting in vigorous and enriched soil. This was more pronounced in the case of the spearmint and peppermint, which also increased the tomato growth. The high soil nutritional status and enhanced plant growth were most prominent when the aromatic plants were left for 28 days to decompose in the soil before sowing. Compared with the mint plants, the rosemary had similar, yet less intense, effects on the soil community, but completely inhibited the growth of the tomato seedlings. Therefore, it is not recommended for use as a soil amendment in tomato seedbeds. Full article

(This article belongs to the Special Issue Plant–Nematode Interaction)

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17 pages, 2644 KiB

Open AccessArticle

Occurrence and Geographic Distribution of Plant-Parasitic Nematodes Associated with Citrus in Morocco and Their Interaction with Soil Patterns

by Btissam Zoubi, Fouad Mokrini, Abdelfattah A. Dababat, Mohammed Amer, Cherki Ghoulam, Rachid Lahlali, Salah-Eddine Laasli, Khalid Khfif, Mustafa Imren, Oumaima Akachoud, Abderrazak Benkebboura, Abdelilah Iraqi Housseini and Ahmed Qaddoury

Life 2022, 12(5), 637; https://doi.org/10.3390/life12050637 - 25 Apr 2022

Cited by 13 | Viewed by 3698

Abstract

Plant-parasitic nematodes (PPNs) are found in citrus plantations throughout the world, but they are considered to be the most problematic pest in Morocco. Citrus fruit quality and yield have been adversely affected by PPNs. Due to data unavailability of nematodes associated with citrus, [...] Read more.

Plant-parasitic nematodes (PPNs) are found in citrus plantations throughout the world, but they are considered to be the most problematic pest in Morocco. Citrus fruit quality and yield have been adversely affected by PPNs. Due to data unavailability of nematodes associated with citrus, a detailed survey was conducted in the main citrus-growing regions of Morocco during 2020–2021 to assess the occurrence, distribution, and diversity of PPNs associated with rhizospheres of citrus trees. In addition, some soil properties have also been assessed for their impact on soil properties. Plant-parasitic nematode diversity was calculated using two ecological indexes, the Shannon diversity index (H′) and the Evenness index (E). The collected soil and root samples were analyzed, and eleven genera and ten species of plant-parasitic nematodes were identified. The results show that the most predominant PPN species were Tylenchulus semipenetrans (88%), Helicotylenchus spp. (75%), Pratylenchus spp. (47%), Tylenchus spp. (51%), and Xiphinema spp. (31%). The results showed that PPN distributions were correlated with soil physicochemical properties such as soil texture, pH levels, and mineral content. Based on the obtained result, it was concluded that besides the direct effects of the host plant, physicochemical factors of the soil could greatly affect PPN communities in citrus growing orchards. Full article

(This article belongs to the Special Issue Plant–Nematode Interaction)

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13 pages, 855 KiB

Open AccessArticle

A Three-Year Plant Study of Salt-Tolerant Transgenic Maize Showed No Effects on Soil Enzyme Activity and Nematode Community

by Xing Zeng, Tongtong Pei, Yongfeng Song, Pei Guo, Huilan Zhang, Xin Li, Hao Li, Hong Di and Zhenhua Wang

Life 2022, 12(3), 412; https://doi.org/10.3390/life12030412 - 11 Mar 2022

Cited by 8 | Viewed by 2257

Abstract

The environmental effects of genetically modified crops are now a global concern. It is important to monitor the potential environmental impact of transgenic corn after commercial release. In rhizosphere soil, plant roots interact with soil enzymes and microfauna, which can be affected by [...] Read more.

The environmental effects of genetically modified crops are now a global concern. It is important to monitor the potential environmental impact of transgenic corn after commercial release. In rhizosphere soil, plant roots interact with soil enzymes and microfauna, which can be affected by the transgenes of genetically modified crops. To determine the long-term impact of transgenic plant cultivation, we conducted a field study for 3 consecutive years (2018–2020) and observed the enzyme activities and nematode populations in plots planted with transgenic maize BQ-2, non-transgenic wild-type maize (Qi319), and inbred line B73. We took soil samples from three cornfields at four different growth stages (V3, V9, R1, and R6 stages); determined soil dehydrogenase, urease, and sucrase activities; and collected and identified soil nematodes to the genus level. The results demonstrated seasonal variations in dehydrogenase, urease, and sucrase activities. However, there was a consistent trend of change. The generic composition and diversity indices of the soil nematodes did not significantly differ, although significant seasonal variation was found in the individual densities of the principal trophic groups and the diversity indices of the nematodes in all three cornfields. The results of the study suggest that a 3-year cultivation of transgenic corn had no significant effects on soil enzyme activity and the soil nematode community. This study provides a theoretical basis for the environmental impact monitoring of transgenic corn. Full article

(This article belongs to the Special Issue Plant–Nematode Interaction)

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15 pages, 1813 KiB

Open AccessArticle

Genome-Wide Association Study of Root-Lesion Nematodes Pratylenchus Species and Crown Rot Fusarium culmorum in Bread Wheat

by Quahir Sohail, Gul Erginbas-Orakci, Fatih Ozdemir, Abdulqader Jighly, Susanne Dreisigacker, Harun Bektas, Nevzat Birisik, Hakan Ozkan and Abdelfattah A. Dababat

Life 2022, 12(3), 372; https://doi.org/10.3390/life12030372 - 4 Mar 2022

Cited by 9 | Viewed by 2857

Abstract

Triticum aestivum L., also known as common wheat, is affected by many biotic stresses. Root diseases are the most difficult to tackle due to the complexity of phenotypic evaluation and the lack of resistant sources compared to other biotic stress factors. Soil-borne pathogens [...] Read more.

Triticum aestivum L., also known as common wheat, is affected by many biotic stresses. Root diseases are the most difficult to tackle due to the complexity of phenotypic evaluation and the lack of resistant sources compared to other biotic stress factors. Soil-borne pathogens such as the root-lesion nematodes caused by the Pratylenchus species and crown rot caused by various Fusarium species are major wheat root diseases, causing substantial yield losses globally. A set of 189 advanced spring bread wheat lines obtained from the International Maize and Wheat Improvement Center (CIMMYT) were genotyped with 4056 single nucleotide polymorphisms (SNP) markers and screened for root-lesion nematodes and crown rot resistance. Population structure revealed that the genotypes could be divided into five subpopulations. Genome-Wide Association Studies were carried out for both resistances to Pratylenchus and Fusarium species. Based on our results, 11 different SNPs on chromosomes 1A, 1B, 2A, 3A, 4A, 5B, and 5D were significantly associated with root-lesion nematode resistance. Seven markers demonstrated association with P. neglectus, while the remaining four were linked to P. thornei resistance. In the case of crown rot, eight different markers on chromosomes 1A, 2B, 3A, 4B, 5B, and 7D were associated with Fusarium crown rot resistance. Identification and screening of root diseases is a challenging task; therefore, the newly identified resistant sources/genotypes could be exploited by breeders to be incorporated in breeding programs. The use of the identified markers in marker-assisted selection could enhance the selection process and cultivar development with root-lesion nematode and crown rot resistance. Full article

(This article belongs to the Special Issue Plant–Nematode Interaction)

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15 pages, 2512 KiB

Open AccessArticle

Response of Soil Nematode Community Structure and Function to Monocultures of Pumpkin and Melon

by Dan Zhao, Yao Wang, Ling Wen, Hongyun Qu, Zuobiao Zhang, Hui Zhang, Yunhe Jia, Juan Wang, Yixin Feng, Yan Li, Fan Yang and Fengjuan Pan

Life 2022, 12(1), 102; https://doi.org/10.3390/life12010102 - 12 Jan 2022

Cited by 7 | Viewed by 2677

Abstract

It is well known that crop monoculture can induce negative effects on soil ecosystems and crop productivity. However, little is known about how vegetable monoculture affects the soil nematode community structure and its relationship with vegetable yields. In this study, the composition, abundance, [...] Read more.

It is well known that crop monoculture can induce negative effects on soil ecosystems and crop productivity. However, little is known about how vegetable monoculture affects the soil nematode community structure and its relationship with vegetable yields. In this study, the composition, abundance, metabolic footprint, and ecological indices of soil nematodes are investigated in monocultures of pumpkin and melon. The relationships between nematode community structure and yields of pumpkin and melon were analyzed by linear regression. Both monoculture soils of pumpkin and melon suppressed the relative abundance of bacterivores but increased the relative abundance of plant parasites. Pumpkin monoculture soils decreased soil nematode diversity but increased the maturity index of plant parasites. Monoculture soils of pumpkin and melon decreased the metabolic footprint of lower- and higher-level trophic groups of the soil food web, respectively. Pumpkin and melon monoculture soils increased the food web indices channel index (CI) but decreased the enrichment index (EI) and the structure index (SI). The monoculture soils of pumpkin and melon led to a more fungal-dominated decomposition pathway and degraded soil food web conditions. The abundance of bacterivores and food web indices EI and SI were positively correlated with soil nutrients and pH, while the abundance of plant parasites and CI were negatively correlated with soil nutrients and pH. Paratylenchus was negatively correlated with pumpkin and melon yields and could be the potential plant parasites threatening pumpkin and melon productions. Redundancy analysis showed that monocultures of pumpkin and melon altered the soil nematode community via soil properties; total N, total P, alkeline-N, and pH were the main driving factors. Full article

(This article belongs to the Special Issue Plant–Nematode Interaction)

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22 pages, 2577 KiB

Open AccessArticle

Biodiversity and Community Analysis of Plant-Parasitic and Free-Living Nematodes Associated with Maize and Other Rotational Crops from Punjab, Pakistan

by Aatika Sikandar, Tabassum Ara Khanum and Yuanyuan Wang

Life 2021, 11(12), 1426; https://doi.org/10.3390/life11121426 - 17 Dec 2021

Cited by 12 | Viewed by 3165

Abstract

Maize (Zea mays L.) is one of Pakistan’s essential staple food crops. Plant-parasitic nematodes (PPNs) are a significant restraint in maize production. However, free-living nematodes (FLNs) provide crucial ecological functions such as suppressing pests and nutrient mineralization. This study aimed to assess [...] Read more.

Maize (Zea mays L.) is one of Pakistan’s essential staple food crops. Plant-parasitic nematodes (PPNs) are a significant restraint in maize production. However, free-living nematodes (FLNs) provide crucial ecological functions such as suppressing pests and nutrient mineralization. This study aimed to assess the community analysis of plant-parasitic and free-living nematodes associated with maize and other rotational crops (those cultivated in sequence with the maize in the same field) from Punjab, Pakistan. The occurrence percentage was observed per 500 g soil for each nematode genus. The present study revealed that 24 species of plant-parasitic and free-living nematodes were identified from maize crops and other rotational crops from 16 localities through Punjab, Pakistan. Nematode communities were analyzed by absolute frequency, relative frequency, relative density, and prominence value, while cluster analysis was based on the presence or absence of nematode in different localities. The overall proportion of plant-parasitic nematodes was 35%, while free-living soil nematodes recovered 65%, out of 210 samples of maize and other rotational crops. Several major genera of plant-parasitic nematodes were reported during the present study viz., Ditylenchus, Filenchus, Helicotylenchus, Hemicriconemoides, Heterodera, Hoplolaimus, Malenchus, Pratylenchus, Psilenchus, Rotylenchulus, Seinura, Telotylenchus, Tylenchorhynchus, and Xiphinema Community relationship revealed the overall dominance of Heterodera zeae, with the highest incidence (55.71%) followed by Tylenchorhynchus elegans (33.33%) and Helicotylenchus certus (24.76%). The results provide valuable information on the community structure of nematodes in maize and other rotational crops of maize in Punjab, Pakistan. Moreover, this data can be used as a preventive measure before PPN incidence results in greater losses on maize. Full article

(This article belongs to the Special Issue Plant–Nematode Interaction)

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16 pages, 3473 KiB

Open AccessArticle

Development of a Species-Specific SCAR-PCR Assay for Direct Detection of Sugar Beet Cyst Nematode (Heterodera schachtii) from Infected Roots and Soil Samples

by Chen Jiang, Yingdong Zhang, Ke Yao, Sulaiman Abdulsalam, Guangkuo Li, Haifeng Gao, Kemei Li, Wenkun Huang, Lingan Kong, Deliang Peng and Huan Peng

Life 2021, 11(12), 1358; https://doi.org/10.3390/life11121358 - 7 Dec 2021

Cited by 6 | Viewed by 3028

Abstract

Sugar beet cyst nematode (SBCN, Heterodera schachtii) is an important nematode that causes significant yield losses of 25–50% or more in most areas of sugar beet production worldwide. Rapid and accurate identification of this species is essential to support decisions on pest [...] Read more.

Sugar beet cyst nematode (SBCN, Heterodera schachtii) is an important nematode that causes significant yield losses of 25–50% or more in most areas of sugar beet production worldwide. Rapid and accurate identification of this species is essential to support decisions on pest management. However, the difference between H. schachtii and other Heterodera spp. based on morphology is a challenging task. In the present study, a SCAR-PCR assay was developed to identify and differentiate H. schachtii in infected root and soil samples. H. schachtii-species-specific SCAR-PCR primers OPA06-HsF and OPA06-HsR were designed from the randomly amplified polymorphic DNA (RAPD) marker amplified with random primer OPA06. The developed primers specifically amplify a 922-bp fragment from the target populations but did not amplify DNA from non-target cyst nematodes including Heterodera, Globodera, Cactodera, and other related species tested in this study. The sensitivity detection indicated that 5 × 10⁻⁴ of a single cyst, 1/320 of a single second-stage juvenile (J2), or 10 pg of genomic DNA could be detected. The assay accurately identifies the different stages of H. schachtii in sugar beet and oilseed rape roots as well as a single J2 in 10 g of soil. Finally, the SCAR-PCR assay detected H. schachtii in seven samples out of the fifteen field samples. The assay will not only be useful for differentiating H. schachtii from mixed populations of Heterodera spp. but also for effective detection of the species directly from infested samples. The assay also requires no expertise in the taxonomy and morphology of the species but serves to improve the diagnosis of H. schachtii in infested fields. Full article

(This article belongs to the Special Issue Plant–Nematode Interaction)

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10 pages, 2882 KiB

Open AccessArticle

First Detection of Ditylenchus destructor Parasitizing Maize in Northeast China

by Fengjuan Pan, Feng Li, Yanzhi Mao, Dan Liu, Aoshuang Chen, Dan Zhao and Yanfeng Hu

Life 2021, 11(12), 1303; https://doi.org/10.3390/life11121303 - 26 Nov 2021

Cited by 6 | Viewed by 2705

Abstract

Maize is one of the most important crops in the world. Heilongjiang province has the largest maize area in China. Plant-parasitic nematodes are important agricultural pests, which cause huge economic losses every year and have attracted global attention. Potato rot nematode Ditylenchus destructor [...] Read more.

Maize is one of the most important crops in the world. Heilongjiang province has the largest maize area in China. Plant-parasitic nematodes are important agricultural pests, which cause huge economic losses every year and have attracted global attention. Potato rot nematode Ditylenchus destructor is a plant-parasitic nematode with a wide range of hosts and strong survival ability in different environments, which brings risks to agricultural production. In 2020, D. destructor was detected in seven maize fields in Heilongjiang province. Morphological identification and molecular approach were used to characterize the isolated D. destructor. The observed morphological and morphometric characteristics were highly similar and consistent with the existing description. The DNA sequencing on the D2/D3 region of the ribosomal DNA 28S and the phylogenetic analysis showed that D. destructor population obtained from maize and other isolates infesting carrot, sweet potato, and potato were in subclade I supported by a 96% bootstrap value. Additionally, the phylogenetic analysis of the ITS rRNA gene sequence further indicated that this D. destructor population from maize clustered in a clade I group and belonged to ITS rRNA haplotype C. An inoculation experiment revealed that D. destructor was pathogenic on the maize seedlings in pots and caused the disease symptoms in the stem base of maize seedlings. This is the first report of D. destructor causing stem rot of maize in Heilongjiang province, and contributes additional information on disease control and safe production of maize in the region. Full article

(This article belongs to the Special Issue Plant–Nematode Interaction)

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10 pages, 790 KiB

Open AccessArticle

Systematic Investigation of Plant-Parasitic Nematodes Associated with Main Subtropical Crops in Guangxi Province, China

by Yi-Xue Mo, Ai-Su Mo, Zhuo-Qiu Qiu, Bing-Xue Li and Hai-Yan Wu

Life 2021, 11(11), 1177; https://doi.org/10.3390/life11111177 - 3 Nov 2021

Cited by 4 | Viewed by 2055

Abstract

Plant parasitic nematodes (PPNs) are a pathogenic group that causes momentous crop yield loss by retarding plant growth and development through plant parasitization. In this study, the distribution of PPNs based on the main crops in Guangxi Province of China was investigated. A [...] Read more.

Plant parasitic nematodes (PPNs) are a pathogenic group that causes momentous crop yield loss by retarding plant growth and development through plant parasitization. In this study, the distribution of PPNs based on the main crops in Guangxi Province of China was investigated. A total of 425 samples of soil or roots from sugarcane, rice, maize, and soybean were collected in 68 counties, and a total of 48 order/family/genera of PPNs were identified, of which some genera were found in more than one crop. A total of 31 order/family/genera of PPNs were found in rice, among which Hirschmanniella was the most abundant, accounting for 79.23%, followed by Tylenchorhynchus (34.43%). Forty order/family/genera were observed in maize, of which the dominant genera were Pratylenchus and Tylenchorhynchus at 45.14% and 32.64%, respectively. In addition, 30 order/family/genera of PPNs were detected from sugarcane, and the percentages of Tylenchorhynchus and Helicotylenchus were 70.42% and 39.44%, respectively. The main crop of Eastern ecological regions was rice, with a high frequency of Hirschmanniella. The greatest frequency of Pratylenchus was found in the Western eco-region, which had a large area of maize. In the Northern eco-region, rice and maize were popular, with abundant Hirschmanniella and Helicotylenchus. In the Central eco-region, Pratylenchus was detected on the main crop of sugarcane. Hirschmanniella (72.94%) was dominant in clay, and Tylenchorhynchus (54.17%) showed the highest frequency in loam. The distribution of PPNs varied with different altitudes. The diversity of this phenomenon was closely related to host plants. These results could improve understanding of the distribution of PPNs and provide important information for controlling PPNs. Full article

(This article belongs to the Special Issue Plant–Nematode Interaction)

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13 pages, 20152 KiB

Open AccessArticle

Evaluation of Scopoletin from Penicillium janthinellum Snef1650 for the Control of Heterodera glycines in Soybean

by Jichen Yan, Zhifu Xing, Piao Lei, Aatika Sikandar, Ruowei Yang, Yuanyuan Wang, Xiaofeng Zhu, Xiaoyu Liu, Haiyan Fan, Yuanhu Xuan, Lijie Chen and Yuxi Duan

Life 2021, 11(11), 1143; https://doi.org/10.3390/life11111143 - 26 Oct 2021

Cited by 5 | Viewed by 2414

Abstract

Soybean cyst nematode (SCN) (Heterodera glycines Ichinohe) is responsible for causing a major soybean disease globally. The fungal strain Penicillium janthinellum Snef1650 was evaluated against H. glycines. However, the effective determinants of the P. janthinellum strain are unknown. By performing pot [...] Read more.

Soybean cyst nematode (SCN) (Heterodera glycines Ichinohe) is responsible for causing a major soybean disease globally. The fungal strain Penicillium janthinellum Snef1650 was evaluated against H. glycines. However, the effective determinants of the P. janthinellum strain are unknown. By performing pot experiments, a functioning compound was isolated from P. janthinellum Snef1650 through organic solvent extraction, semi-preparative HPLC, Sephadex LH-20 column chromatography, and silica gel column chromatography, and the isolated compound was identified to be scopoletin through 1H NMR, 13C NMR, and HPLC–MS. The pot experiments indicated that the treatment of soybean seeds with scopoletin drastically reduced the SCN population. The field experiments performed in 2017 and 2018 revealed that scopoletin decreased over 43.7% juveniles in the roots and over 61.55% cysts in the soil. Scopoletin treatment also promoted soybean growth and improved its yield, with an increase in plot yield by >5.33%. Scopoletin obtained from P. janthinellum Snef1650 could be used as an anti-H. glycines biocontrol agent. Full article

(This article belongs to the Special Issue Plant–Nematode Interaction)

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Review

Jump to: Editorial, Research

17 pages, 1542 KiB

Open AccessReview

Exploiting Plant–Phytonematode Interactions to Upgrade Safe and Effective Nematode Control

by Mahfouz M. M. Abd-Elgawad

Life 2022, 12(11), 1916; https://doi.org/10.3390/life12111916 - 17 Nov 2022

Cited by 9 | Viewed by 2209

Abstract

Plant-parasitic nematodes (PPNs) bring about substantial losses of economic crops globally. With the environmental and health issues facing the use of chemical nematicides, research efforts should focus on providing economically effective and safe control methods. The sound exploitation of plant-PPN interactions is fundamental [...] Read more.

Plant-parasitic nematodes (PPNs) bring about substantial losses of economic crops globally. With the environmental and health issues facing the use of chemical nematicides, research efforts should focus on providing economically effective and safe control methods. The sound exploitation of plant-PPN interactions is fundamental to such efforts. Initially, proper sampling and extraction techniques should be followed to avoid misleading nematode data. Recent evolutions in plant-PPN interactions can make use of diverse non-molecular and molecular approaches to boost plant defenses. Therefore, PPN control and increasing crop yields through single, sequential, dual-purpose, and simultaneous applications of agricultural inputs, including biocontrol agents, should be seriously attempted, especially within IPM schemes. The use of biologicals would ideally be facilitated by production practices to solve related issues. The full investment of such interactions should employ new views of interdisciplinary specialties in the relevant modern disciplines to optimize the PPN management. Having an accurate grasp of the related molecular events will help in developing tools for PPN control. Nonetheless, the currently investigated molecular plant-PPN interactions favoring plant responses, e.g., resistance genes, RNA interference, marker-assisted selection, proteinase inhibitors, chemo-disruptive peptides, and plant-incorporated protectants, are key factors to expanding reliable management. They may be applied on broader scales for a substantial improvement in crop yields. Full article

(This article belongs to the Special Issue Plant–Nematode Interaction)

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22 pages, 743 KiB

Open AccessReview

Xenorhabdus spp.: An Overview of the Useful Facets of Mutualistic Bacteria of Entomopathogenic Nematodes

by Mahfouz M. M. Abd-Elgawad

Life 2022, 12(9), 1360; https://doi.org/10.3390/life12091360 - 31 Aug 2022

Cited by 20 | Viewed by 3508

Abstract

Mounting concern over the misuse of chemical pesticides has sparked broad interest for safe and effective alternatives to control plant pests and pathogens. Xenorhabdus bacteria, as pesticidal symbionts of the entomopathogenic nematodes Steinernema species, can contribute to this solution with a treasure trove [...] Read more.

Mounting concern over the misuse of chemical pesticides has sparked broad interest for safe and effective alternatives to control plant pests and pathogens. Xenorhabdus bacteria, as pesticidal symbionts of the entomopathogenic nematodes Steinernema species, can contribute to this solution with a treasure trove of insecticidal compounds and an ability to suppress a variety of plant pathogens. As many challenges face sound exploitation of plant–phytonematode interactions, a full useful spectrum of such interactions should address nematicidal activity of Xenorhabdus. Steinernema–Xenorhabdus complex or Xenorhabdus individually should be involved in mechanisms underlying the favorable side of plant–nematode interactions in emerging cropping systems. Using Xenorhabdus bacteria should earnestly be harnessed to control not only phytonematodes, but also other plant pests and pathogens within integrated pest management plans. This review highlights the significance of fitting Xenorhabdus-obtained insecticidal, nematicidal, fungicidal, acaricidal, pharmaceutical, antimicrobial, and toxic compounds into existing, or arising, holistic strategies, for controlling many pests/pathogens. The widespread utilization of Xenorhabdus bacteria, however, has been slow-going, due to costs and some issues with their commercial processing. Yet, advances have been ongoing via further mastering of genome sequencing, discovering more of the beneficial Xenorhabdus species/strains, and their successful experimentations for pest control. Their documented pathogenicity to a broad range of arthropods and pathogens and versatility bode well for useful industrial products. The numerous beneficial traits of Xenorhabdus bacteria can facilitate their integration with other tactics for better pest/disease management programs. Full article

(This article belongs to the Special Issue Plant–Nematode Interaction)

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