Marine Polysaccharides and Oligosaccharides: Extraction and Biological Activities

A special issue of Marine Drugs (ISSN 1660-3397). This special issue belongs to the section "Marine Pharmacology".

Deadline for manuscript submissions: 31 January 2025 | Viewed by 1345

Special Issue Editors


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Guest Editor
Laboratoire de Biotechnologie et Chimie Marines, Université Bretagne Sud, Vannes, France
Interests: macroalgae; polysaccharides; bio-activity; analytical chemistry; fractionation; integrated multi-trophic aquaculture

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Guest Editor
Laboratoire de Biotechnologie et Chimie Marines, Université Bretagne Sud, Vannes, France
Interests: phycology; marine molecules; extraction eco-friendly processes; antiviral agents; SAR studies
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Special Issue Information

Dear Colleagues,

The marine environment holds a huge and diverse group of organisms with extraordinary capacities to thrive within challenging conditions. Their unique physiological and metabolic pathways allow them to produce a great diversity of molecules. Marine polysaccharides represent a broad spectrum of macromolecules with diverse and unusual structures; they play multiple roles and have extensive bioactive processes. At present, the most widespread uses are in the colloid industry for foods, feeds, pharmaceuticals, and cosmetics. However, its potential applications are numerous, with the potential to contribute to the marine circular economy and generate improvements in human welfare. Without a doubt, there are polysaccharides that are currently unknown, and our understanding of these is far from being concluded.

This Special Issue welcomes original research and comprehensive reviews in the field of marine polysaccharides, olisaccharides, and their derivatives with potential biological activities, such as antiviral, anticancer, anti-inflammatory, antioxidant, etc. Biochemical and structural characterization is encouraged due to its structural complexity and diversity. Marine polysaccharides and aligosaccharides sourcing is included, but is not exclusive to aquaculture, biotechnology, or synthetic biology. We hope that this Special Issue will provide the state of the art in marine polysaccharide research.

Dr. Hugo Pliego-Cortés
Prof. Dr. Nathalie Bourgougnon
Guest Editors

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Keywords

  • marine polysaccharide
  • oligosaccharides
  • extraction
  • isolation
  • structural analysis
  • biological activity
  • bioprocessing
  • biorefinery
  • marine organism
  • aquaculture

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Published Papers (1 paper)

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Research

20 pages, 7887 KiB  
Article
Degradation of Natural Undaria pinnatifida into Unsaturated Guluronic Acid Oligosaccharides by a Single Alginate Lyase
by Hui Wang, Jiaqi Wen, Nuraliya Ablimit, Kun Deng, Wenzhuo Wang and Wei Jiang
Mar. Drugs 2024, 22(10), 453; https://doi.org/10.3390/md22100453 - 2 Oct 2024
Viewed by 981
Abstract
Here, we report on a bifunctional alginate lyase (Vnalg7) expressed in Pichia pastoris, which can degrade natural Undaria pinnatifida into unsaturated guluronic acid di- and trisaccharide without pretreatment. The enzyme activity of Vnalg7 (3620.00 U/mL-culture) was 15.81-fold higher than that of the [...] Read more.
Here, we report on a bifunctional alginate lyase (Vnalg7) expressed in Pichia pastoris, which can degrade natural Undaria pinnatifida into unsaturated guluronic acid di- and trisaccharide without pretreatment. The enzyme activity of Vnalg7 (3620.00 U/mL-culture) was 15.81-fold higher than that of the original alg (228.90 U/mL-culture), following engineering modification. The degradation rate reached 52.75%, and reducing sugar reached 30.30 mg/mL after combining Vnalg7 (200.00 U/mL-culture) and 14% (w/v) U. pinnatifida for 6 h. Analysis of the action mode indicated that Vnalg7 could degrade many substrates to produce a variety of unsaturated alginate oligosaccharides (AOSs), and the minimal substrate was tetrasaccharide. Site-directed mutagenesis showed that Glu238, Glu241, Glu312, Arg236, His307, Lys414, and Tyr418 are essential catalytic sites, while Glu334, Glu344, and Asp311 play auxiliary roles. Mechanism analysis revealed the enzymatic degradation pattern of Vnalg7, which mainly recognizes and attacks the third glycosidic linkage from the reducing end of oligosaccharide substrate. Our findings provide a novel alginate lyase tool and a sustainable and commercial production strategy for value-added biomolecules using seaweeds. Full article
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