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New Trends in Bioprinting
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New Trends in Bioprinting

A special issue of Materials (ISSN 1996-1944). This special issue belongs to the section "Biomaterials".

Deadline for manuscript submissions: closed (10 September 2022) | Viewed by 5565

Special Issue Editors

Dr. Carmelo De Maria

E-Mail Website
Guest Editor
Research Center "E. Piaggio", Department of Information Engineering, University of Pisa, Largo Lucio Lazzarino 1, 56122 Pisa, Italy
Interests: multiscale and multimaterial bioprinting; mathematical modeling of bioprinting processes
Prof. Dr. Giovanni Vozzi

E-Mail Website
Guest Editor
Research Center "E. Piaggio", Department of Information Engineering, University of Pisa, Largo Lucio Lazzarino 1, 56122 Pisa, Italy
Interests: bioengineering; biomedical engineering; tissue engineering; microfabrication; bioreactors for tissue culture; microactuators fabrication
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Thomas Boland

E-Mail Website
Guest Editor
Metallurgical, Materials and Biomedical Engineering Dpt, College of Engineering, University of Texas at El Paso, 500 West University Avenue, El Paso, TX 79968, USA
Interests: inkjet bioprinting

Special Issue Information

Dear Colleagues,

Bioprinting has been defined as the computer-aided process for producing bioengineered constructs, composed of living and nonliving materials, mimicking tissues or organs with high throughput and reproducibility. This process, started as a key enabling approach for tissue engineering and regenerative medicine, has evolved into a mature research field. For example, bioprinted in vitro models are now under evaluation for replacing in vivo models, and bioprinting companies, with sustainable business models, offer both bioinks and bioprinters with affordable prices, promoting applications in translational medicine.  

Taking advantage of the interaction with other research fields such as robotics, additive manufacturing, green chemistry, multiple innovations are continuously deployed, with impacts in biology and biotechnology, for producing, e.g., biosensors, biological machines, supports for catalysis, and tools for life-in-space exploration.

The Guest Editor of this Special Issue will consider articles that present the recent advances in the research and applications of bioprinting, with a particular interest in:

  • Innovative bioprinting technologies, including 4D bioprinting;
  • In situ bioprinting;
  • Mathematical modeling of bioprinting processes;
  • Bioprinting of sustainable biomaterial inks and bioinks;
  • Molecular analysis of bioprinted constructs;
  • Bioprinting applications for space exploration;
  • Bioprinting applications in biotechnology.

Dr. Carmelo De Maria
Prof. Dr. Giovanni Vozzi
Prof. Dr. Thomas Boland
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Materials is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • bioprinting
  • biofabrication, in vitro model
  • scaffold
  • bioink
  • tissue engineering
  • regenerative medicine
  • biotechnology

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Published Papers (3 papers)

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Research

13 pages, 5928 KiB  
Article
Design and Fabrication of Mature Engineered Pre-Cardiac Tissue Utilizing 3D Bioprinting Technology and Enzymatically Crosslinking Hydrogel
by Shintaroh Iwanaga, Yuta Hamada, Yoshinari Tsukamoto, Kenichi Arai, Taketoshi Kurooka, Shinji Sakai and Makoto Nakamura
Materials 2022, 15(22), 7928; https://doi.org/10.3390/ma15227928 - 9 Nov 2022
Cited by 9 | Viewed by 2038
Abstract
The fabrication of mature engineered cardiac tissue is one of the major challenges in cardiac tissue engineering. For this purpose, we attempted to apply the 3D bioprinting approach. Aiming to construct an oriented tissue, a fine fiber-shaped scaffold with a support structure was [...] Read more.
The fabrication of mature engineered cardiac tissue is one of the major challenges in cardiac tissue engineering. For this purpose, we attempted to apply the 3D bioprinting approach. Aiming to construct an oriented tissue, a fine fiber-shaped scaffold with a support structure was first designed using CAD software. Then, a 3D bioprinter and cell-adhesive bio-inks were utilized to fabricate this structure. The cell-adhesive bio-inks were synthesized by combining sodium alginate and gelatin with tyramine, respectively, to form pre-gel materials that allow enzymatic crosslinking by horseradish peroxidase. By absorbance measurements, we confirmed that the tyramine modification rate of each polymer was 0.535 mmol/g-alginate and 0.219 mmol/g-gelatin. The width of the fiber-shaped scaffold was 216.8 ± 24.3 μm for the fabricated scaffold, while the design value was 200 μm. After 3D printing and adhesion-adding treatment of the scaffold with these bio-ink materials, cardiomyocytes were seeded and cultured. As a result, the cells spread onto the scaffold, and the entire pre-tissue contracted synchronously by day 6 of culture, showing a greater pulsatility than in the early days. Video analysis showed that the beating rate of pre-myocardial tissue on day 6 was 31 beats/min. In addition, we confirmed that the cardiomyocytes partially elongated along the long axis of the fiber-shaped scaffold in the pre-tissue cultured for 15 days by staining actin, suggesting the possibility of cell orientation. Furthermore, treatment with adrenaline resulted in a 7.7-fold increase in peak beating rate compared to that before treatment (from 6 beats/min to 46 beats/min), confirming the responsiveness of the pre-tissues to the drug. These results indicate that 3D bioprinting effectively produces mature cultured myocardial tissue that is oriented, contracts synchronously, and is responsive to drugs. Full article
(This article belongs to the Special Issue New Trends in Bioprinting)
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11 pages, 3210 KiB  
Article
Assessment of Angiogenesis and Cell Survivability of an Inkjet Bioprinted Biological Implant in an Animal Model
by Beu P. Oropeza, Carlos Serna III, Michael E. Furth, Luis H. Solis, Cesar E. Gonzalez, Valeria Altamirano, Daisy C. Alvarado, Jesus A. Castor, Jesus A. Cedeno, Dante Chaparro Vega, Octavio Cordova, Isaac G. Deaguero, Erwin I. Delgado, Mario F. Garcia Duarte, Mirsa Gonzalez Favela, Alba J. Leyva Marquez, Emilio S. Loera, Gisela Lopez, Fernanda Lugo, Tania G. Miramontes, Erik Munoz, Paola A. Rodriguez, Leila M. Subia, Arahim A. Zuniga Herrera and Thomas Bolandadd Show full author list remove Hide full author list
Materials 2022, 15(13), 4468; https://doi.org/10.3390/ma15134468 - 24 Jun 2022
Cited by 3 | Viewed by 1899
Abstract
The rapidly growing field of tissue engineering hopes to soon address the shortage of transplantable tissues, allowing for precise control and fabrication that could be made for each specific patient. The protocols currently in place to print large-scale tissues have yet to address [...] Read more.
The rapidly growing field of tissue engineering hopes to soon address the shortage of transplantable tissues, allowing for precise control and fabrication that could be made for each specific patient. The protocols currently in place to print large-scale tissues have yet to address the main challenge of nutritional deficiencies in the central areas of the engineered tissue, causing necrosis deep within and rendering it ineffective. Bioprinted microvasculature has been proposed to encourage angiogenesis and facilitate the mobility of oxygen and nutrients throughout the engineered tissue. An implant made via an inkjet printing process containing human microvascular endothelial cells was placed in both B17-SCID and NSG-SGM3 animal models to determine the rate of angiogenesis and degree of cell survival. The implantable tissues were made using a combination of alginate and gelatin type B; all implants were printed via previously published procedures using a modified HP inkjet printer. Histopathological results show a dramatic increase in the average microvasculature formation for mice that received the printed constructs within the implant area when compared to the manual and control implants, indicating inkjet bioprinting technology can be effectively used for vascularization of engineered tissues. Full article
(This article belongs to the Special Issue New Trends in Bioprinting)
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18 pages, 4221 KiB  
Article
Novel Combinatorial Strategy Using Thermal Inkjet Bioprinting, Chemotherapy, and Radiation on Human Breast Cancer Cells; an In-Vitro Cell Viability Assessment
by Aleli Campbell, Denisse A. Gutierrez, Colin Knight, Charlotte M. Vines, Rosalinda Heydarian, Alexander Philipovskiy, Armando Varela-Ramirez and Thomas Boland
Materials 2021, 14(24), 7864; https://doi.org/10.3390/ma14247864 - 19 Dec 2021
Cited by 7 | Viewed by 2872
Abstract
Background: Breast cancer (BC) continues to have the second highest mortality amongst women in the United States after lung cancer. For 2021, the American Cancer Association predicted 281,550 new invasive breast cancer cases besides 49,290 new cases of non-invasive breast cancer and 43,600 [...] Read more.
Background: Breast cancer (BC) continues to have the second highest mortality amongst women in the United States after lung cancer. For 2021, the American Cancer Association predicted 281,550 new invasive breast cancer cases besides 49,290 new cases of non-invasive breast cancer and 43,600 deaths from the metastatic disease. A treatment modality is radiation therapy, which is given for local control as well as palliation of patient symptoms. The initial step of new drug development is in-vitro cell studies, which help describe new drug properties and toxicities. However, these models are not optimal, and better ones have yet to be determined. This study uses bioprinting technology to elucidate the sensitivity of tumor cells to the combination of palbociclib (PD) and letrozole (Let) treatment. We hypothesize that this technology could serve as a model to predict treatment outcomes more efficiently. Methods: The breast cancer cell lines MCF7 and MDA-MB-231 as well as the normal breast epithelial cell line, MCF-10A, were treated with PD-Let with and without radiotherapy (RT), and cell viability was compared in pairwise fashion for thermally inkjet bioprinted (TIB) and manually seeded (MS) cells. Results: In absence of radiation, the TIB MCF7 cells have 2.5 times higher viability than manually seeded (MS) cells when treated with 100 µM palbociclib and 10 µM letrozole, a 36% higher viability when treated with 50 µM palbociclib and 10 µM letrozole, and an 8% higher viability when treated with 10 µM palbociclib and 10 µM letrozole. With 10 Gy of radiation, TIB cells had a 45% higher survival rate than MS cells at the lowest palbociclib concentration and a 29% higher survival rate at the intermediate palbociclib concentration. Without radiation treatment, at a concentration of 10 μM PD-Let, TIB MDA-MB-231 cells show a 8% higher viability than MS cells when treated with 10 µM PD and 10 µM Let; at higher drug concentrations, the differences disappeared, but some 1.7% of the TIB MDA-MB-231 cells survived exposure to 150 μM of PD + 10 μM letrozole vs. none of the MS cells. These cells are more radiation sensitive than the other cell lines tested and less sensitive to the combo drug treatments. We observed an 18% higher survival of TIB MCF-10A cells without radiation treatment when exposed to 10 μM PD + 10 μM Let but no difference in cell survival between the two groups when radiation was applied. Independent of growth conditions, TIB cells did not show more resistance to radiation treatment than MS cells, but a higher resistance to the combo treatment was observed, which was most pronounced in the MCF-7 cell line. Conclusion: Based on these results, we suggest that TIB used in in-vitro models could be a feasible strategy to develop and/or test new anticancer drugs. Full article
(This article belongs to the Special Issue New Trends in Bioprinting)
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