Biosensors for Pathogen Detection, Volume II

A special issue of Micromachines (ISSN 2072-666X). This special issue belongs to the section "B:Biology and Biomedicine".

Deadline for manuscript submissions: closed (15 August 2021) | Viewed by 5732

Special Issue Editor


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Guest Editor
Department of Agriculture, Food, Environmental and Animal Sciences Via Sondrio 2/A, Università degli Studi di Udine, 33100 Udine, Italy
Interests: genosensors; aptasensors; molecular biology; PCR; DNA probes; aptamers
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Special Issue Information

Dear Colleagues,

The demands of the food industry for the elimination of diseases caused by the presence of pathogen microorganisms in food, and the demands of breeding farms for rapid diagnoses has led to the development of detection methods mostly based on molecular biology, nanotechnology, and nanomaterials. DNA biosensors utilizing optical, electrochemical, and acoustic transducers have demonstrated their feasibility for diagnostic purposes in pathogen detection as they are rapid, specific, sensitive, and cheap. The latest advancements in oligonucleotide selection (SELEX and SAM) have opened new frontiers in this field, proposing the utilization of specific aptamers, that allow the direct detection of cells using a label-free method. The application of aptamers has been increasing in recent years.

DNA-based biosensors can recognize various pathogens, bacteria, viruses, and fungi that can affect humans, animals, plants, food, water, and the environment; moreover, the biosensor market continues to expand significantly from the 2 billion dollars it was valued at in 2016 making this sector attractive for technological industries especially for the production of miniaturized biosensors.

This Special Issue will merge new/recent methods that can be useful in pathogen detection for the development of point-of-care (PoC) devices, and for the development of simple protocols.

Authors are invited to contribute original research papers, review articles, and short communications that focus on the development and utilization of novel methods to assure rapid, simple, and cheap detection of pathogens in food, breeding farms, water, environment, and also for clinical applications.

Prof. Dr. Marisa Manzano
Guest Editor

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Keywords

  • Pathogen Detection
  • DNA Sensors
  • Nucleic Acids
  • Aptamers
  • Optical Biosensors
  • Electrochemical Biosensors
  • Acoustic Biosensors
  • Nanomaterials
  • Point of Care
  • Lab-on-Chip

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Published Papers (1 paper)

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Research

14 pages, 7346 KiB  
Article
A Microfluidic Biosensor Based on Magnetic Nanoparticle Separation, Quantum Dots Labeling and MnO2 Nanoflower Amplification for Rapid and Sensitive Detection of Salmonella Typhimurium
by Li Hao, Li Xue, Fengchun Huang, Gaozhe Cai, Wuzhen Qi, Miao Zhang, Qing’an Han, Zengli Wang and Jianhan Lin
Micromachines 2020, 11(3), 281; https://doi.org/10.3390/mi11030281 - 9 Mar 2020
Cited by 48 | Viewed by 5119
Abstract
Screening of foodborne pathogens is an effective way to prevent microbial food poisoning. A microfluidic biosensor was developed for rapid and sensitive detection of Salmonella Typhimurium using quantum dots (QDs) as fluorescent probes for sensor readout and manganese dioxide nanoflowers (MnO2 NFs) [...] Read more.
Screening of foodborne pathogens is an effective way to prevent microbial food poisoning. A microfluidic biosensor was developed for rapid and sensitive detection of Salmonella Typhimurium using quantum dots (QDs) as fluorescent probes for sensor readout and manganese dioxide nanoflowers (MnO2 NFs) and as QDs nanocarriers for signal amplification. Prior to testing, amino-modified MnO2 nanoflowers (MnO2-NH2 NFs) were conjugated with carboxyl-modified QDs through EDC/NHSS method to form MnO2-QD NFs, and MnO2-QD NFs were functionalized with polyclonal antibodies (pAbs) to form MnO2-QD-pAb NFs. First, the mixture of target Salmonella Typhimurium cells and magnetic nanoparticles (MNPs) modified with monoclonal antibodies (mAbs) was injected with MnO2-QD-pAb NFs into a microfluidic chip to form MNP-bacteria-QD-MnO2 complexes. Then, glutathione (GSH) was injected to dissolve MnO2 on the complexes into Mn2+, resulting in the release of QDs. Finally, fluorescent intensity of the released QDs was measured using the fluorescent detector to determine the amount of Salmonella. A linear relationship between fluorescent intensity and bacterial concentration from 1.0 × 102 to 1.0 × 107 CFU/mL was found with a low detection limit of 43 CFU/mL and mean recovery of 99.7% for Salmonella in spiked chicken meats, indicating the feasibility of this biosensor for practical applications. Full article
(This article belongs to the Special Issue Biosensors for Pathogen Detection, Volume II)
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