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Microorganisms
Special Issues
Feature Papers in Biotechnology of Microbial Enzymes
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Feature Papers in Biotechnology of Microbial Enzymes

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Microbial Biotechnology".

Deadline for manuscript submissions: closed (31 January 2023) | Viewed by 7530

Special Issue Editor

Prof. Dr. Dietmar Haltrich

E-Mail Website
Guest Editor
Department of Food Sciences and Technology, BOKU University of Natural Resources and Life Sciences, Muthgasse 18, A-1190 Wien, Austria
Interests: microbial biotechnology; enzyme-based processes; biocatalysis; (carbohydrate) oxidoreductases; glycoside hydrolases; oligosaccharides; lactic acid bacteria; recombinant proteins
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Microbial enzymes play an integral and important role in a number of different biotechnological applications, ranging from analytics and medicine to industry. In addition to their well-established applications in these areas, recent developments in the fields of molecular biology, protein chemistry, and enzyme engineering have significantly increased the use of enzymes as biocatalysts in biotechnology. Microbial enzymes and, especially, biocatalysis have developed enormously in the last decade and now offer solutions for the sustainable production of chiral and highly functionalized molecules. Recent advances in oxidative enzymes have furthermore boosted research towards biorefineries and biofuels. This enormous progress is based on new approaches for the screening and identification of novel microbial enzymes, various methods to efficiently (over)produce enzymes in an economic way, various techniques to tailor enzymes with respect to desired or novel properties, as well as a wealth of structural data on enzymes. The successful use of enzymes in industry and biocatalysis requires a transdisciplinary expertise, ranging from biochemistry, to biotechnology, micromolecular and structural biology, process engineering/enzyme reactors, and thus provides a dynamic environment, which will fuel new applications and future innovations. This dynamic interplay between different scientific areas will also be reflected in this Special Issue on the structure, function, and discovery of microbial enzymes, both in traditional fields and in novel industrial applications.

Prof. Dr. Dietmar Haltrich
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • microbial enzymes
  • biocatalysis
  • enzyme engineering
  • structure/function relationships of enzymes
  • production of recombinant enzymes

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Published Papers (2 papers)

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Research

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20 pages, 3275 KiB  
Article
Construction and Application of a Plasmid-Based Signal Peptide Library for Improved Secretion of Recombinant Proteins with Priestia megaterium
by Janine Mayer, Tobias Knuuti, Lisa Baumgarten, Elise Menke, Lena Bischoff, Boyke Bunk and Rebekka Biedendieck
Microorganisms 2022, 10(4), 777; https://doi.org/10.3390/microorganisms10040777 - 5 Apr 2022
Cited by 2 | Viewed by 2719
Abstract
The secretion of recombinant proteins plays an important role in their economic production and purification. The secretion efficiency depends on the responsible signal peptide (SP) in combination with the target protein and the given host and cannot be predicted so far. Due to [...] Read more.
The secretion of recombinant proteins plays an important role in their economic production and purification. The secretion efficiency depends on the responsible signal peptide (SP) in combination with the target protein and the given host and cannot be predicted so far. Due to its high plasmid stability, the lack of alkaline extracellular proteases and only few contaminating extracellular host proteins, Priestia megaterium provides a promising alternative to common Bacillus species. For the development of an easy and fast cloning and screening system to identify the SP best suited to a distinct protein, a plasmid-based SP library containing all predicted 182 Sec-dependent SPs from P. megaterium was established. The splitting of the SPs into 10 groups of individual multi-SP plasmids (pMSPs) allows their grouped amplification and application in screening approaches. The functionality of the whole library was demonstrated by enhancing the amount of the already well-secreted α-amylase AmyE by 1.6-fold. The secretion of a novel penicillin G acylase, which remained as insoluble protein inside the cells, as its native SP is unsuitable for secretion in P. megaterium, could be enhanced even up to 29-fold. Overall, only around 170 recombinant P. megaterium clones based on 50 inserted SPs had to be screened to achieve sufficient amounts for further enzyme characterizations. Thus, this newly developed plasmid-based genetic tool applicable for P. megaterium and also other Bacillus species facilitates the identification of suitable SPs for secretion of recombinant proteins. Full article
(This article belongs to the Special Issue Feature Papers in Biotechnology of Microbial Enzymes)
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Review

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33 pages, 1700 KiB  
Review
Lignocellulolytic Biocatalysts: The Main Players Involved in Multiple Biotechnological Processes for Biomass Valorization
by Ana Laura Totti Benatti and Maria de Lourdes Teixeira de Moraes Polizeli
Microorganisms 2023, 11(1), 162; https://doi.org/10.3390/microorganisms11010162 - 8 Jan 2023
Cited by 26 | Viewed by 3819
Abstract
Human population growth, industrialization, and globalization have caused several pressures on the planet’s natural resources, culminating in the severe climate and environmental crisis which we are facing. Aiming to remedy and mitigate the impact of human activities on the environment, the use of [...] Read more.
Human population growth, industrialization, and globalization have caused several pressures on the planet’s natural resources, culminating in the severe climate and environmental crisis which we are facing. Aiming to remedy and mitigate the impact of human activities on the environment, the use of lignocellulolytic enzymes for biofuel production, food, bioremediation, and other various industries, is presented as a more sustainable alternative. These enzymes are characterized as a group of enzymes capable of breaking down lignocellulosic biomass into its different monomer units, making it accessible for bioconversion into various products and applications in the most diverse industries. Among all the organisms that produce lignocellulolytic enzymes, microorganisms are seen as the primary sources for obtaining them. Therefore, this review proposes to discuss the fundamental aspects of the enzymes forming lignocellulolytic systems and the main microorganisms used to obtain them. In addition, different possible industrial applications for these enzymes will be discussed, as well as information about their production modes and considerations about recent advances and future perspectives in research in pursuit of expanding lignocellulolytic enzyme uses at an industrial scale. Full article
(This article belongs to the Special Issue Feature Papers in Biotechnology of Microbial Enzymes)
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