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Labeling Biosensors

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Biosensors".

Deadline for manuscript submissions: closed (30 November 2020) | Viewed by 10548

Special Issue Editors


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Guest Editor
National Institute of Advanced Industrial Science and Technology, Sensing System Research Center, Tokyo, Japan
Interests: biosensors; immunosensors; optical sensors; near-field optics; surface plasmon resonance; waveguide; nanostructures
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8560, Japan
Interests: bioanalytical chemistry; biosensor; lab on a chip; electrochemistry; surface plasmon resonance; epigenetics
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
School of Science and Technology, Kwansei Gakuin University, Sanda, Japan
Interests: nanobiotechnology; spectroscopy; fluorescence; polymer physicalchemistry; plasmonic chip; bio-detection

Special Issue Information

Dear Colleagues,

Biosensors are widely used in various fields such as diagnosis, drug discovery research, biomarker search, and basic research in biotechnology. One of the most widely used biosensors is a label-based immunological biosensor. Biosensors are required to have high sensitivity, high accuracy, and a wide dynamic range. In labeling biosensors, various techniques and various labels have been developed to satisfy these requirements. The easiest and most widely used labeled biosensor would be immunochromatography, also known as a lateral flow immunoassay. In recent years, since the performance of antibodies has been improved, very high-sensitivity immunochromatography kits have been available for some antigens. Enzyme-linked immunosorbent assay (ELISA) can be said to be the most widely used labeling biosensor in laboratories. ELISA is not only highly sensitive but also highly quantitative. In addition, a number of labeling biosensors using a fluorescent substance or a chemiluminescent substance as a label have been developed. Furthermore, a sensing principle and a sensing device that perform detection with higher sensitivity using these labels have been developed. In recent years, digital assays capable of detecting aM levels have attracted attention. This Special Issue focuses on the development of various labels and enzymes that support biosensing technologies and a labeling sensing system with features such as small size, high sensitivity, and easy operation. We welcome original research contributions and state-of-the-art reviews from academia and industry.

Dr. Makoto Fujimaki
Dr. Ryoji Kurita
Prof. Dr. Keiko Tawa
Guest Editors

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Keywords

  • fluorescent labels
  • nanoparticles
  • enzymes
  • optical biosensors
  • nearfield optics
  • digital assay

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Published Papers (2 papers)

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Review

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20 pages, 53049 KiB  
Review
Ultrasensitive Materials for Electrochemical Biosensor Labels
by Aneesh Koyappayil and Min-Ho Lee
Sensors 2021, 21(1), 89; https://doi.org/10.3390/s21010089 - 25 Dec 2020
Cited by 39 | Viewed by 5448
Abstract
Since the fabrication of the first electrochemical biosensor by Leland C. Clark in 1956, various labeled and label-free sensors have been reported for the detection of biomolecules. Labels such as nanoparticles, enzymes, Quantum dots, redox-active molecules, low dimensional carbon materials, etc. have been [...] Read more.
Since the fabrication of the first electrochemical biosensor by Leland C. Clark in 1956, various labeled and label-free sensors have been reported for the detection of biomolecules. Labels such as nanoparticles, enzymes, Quantum dots, redox-active molecules, low dimensional carbon materials, etc. have been employed for the detection of biomolecules. Because of the absence of cross-reaction and highly selective detection, labeled biosensors are advantageous and preferred over label-free biosensors. The biosensors with labels depend mainly on optical, magnetic, electrical, and mechanical principles. Labels combined with electrochemical techniques resulted in the selective and sensitive determination of biomolecules. The present review focuses on categorizing the advancement and advantages of different labeling methods applied simultaneously with the electrochemical techniques in the past few decades. Full article
(This article belongs to the Special Issue Labeling Biosensors)
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9 pages, 2099 KiB  
Letter
Sensitive Detection of C-Reactive Protein by One-Step Method Based on a Waveguide-Mode Sensor
by Hiroki Ashiba, Chiaki Oyamada, Kazuya Hosokawa, Koji Ueno and Makoto Fujimaki
Sensors 2020, 20(11), 3195; https://doi.org/10.3390/s20113195 - 4 Jun 2020
Cited by 6 | Viewed by 3624
Abstract
One-step biosensing methods enable the quick and simplified detection of biological substances. In this study, we developed a sensitive one-step method on the basis of a waveguide-mode sensor, which is an optical sensor utilizing waveguide-mode resonance and evanescent light. Streptavidin-conjugated and gold-nanoparticle-conjugated antibodies [...] Read more.
One-step biosensing methods enable the quick and simplified detection of biological substances. In this study, we developed a sensitive one-step method on the basis of a waveguide-mode sensor, which is an optical sensor utilizing waveguide-mode resonance and evanescent light. Streptavidin-conjugated and gold-nanoparticle-conjugated antibodies were reacted with a target substance and applied onto a biotinylated sensing plate. The target substance was detected by observing changes in sensor signals caused by binding the immunocomplex to the sensing surface. Performance of the developed one-step method was examined using a C-reactive protein (CRP) as a target substance. A sensor signal corresponding to the concentration of CRP was obtained. The minimal detectable CRP concentration of the developed method was 10 pM. The developed method greatly simplifies quantitative protein detection without reducing sensitivity. Full article
(This article belongs to the Special Issue Labeling Biosensors)
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