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Article

Chemical Composition of the Essential Oils of Cyperus rotundus L. from South Africa

by
Oladipupo A. Lawal
and
Adebola O. Oyedeji
*
Department of Chemistry, University of Zululand, KwaDlangezwa 3886, South Africa
*
Author to whom correspondence should be addressed.
Molecules 2009, 14(8), 2909-2917; https://doi.org/10.3390/molecules14082909
Submission received: 12 June 2009 / Revised: 31 July 2009 / Accepted: 6 August 2009 / Published: 6 August 2009
Versions Notes

Abstract

:
The essential oils from the rhizomes of Cyperus rotundus L. collected from two different locations (Empangeni-A and KwaDlangezwa-B; both in the Kwa-Zulu Natal Province of South Africa) were obtained by hydrodistillation and analyzed by capillary GC and GC/MS. Forty-one and 43 components were identified, representing 89.9% and 92.0% of sample A and sample B, respectively. α-Cyperone (11.0%), myrtenol (7.9%), caryophyllene oxide (5.4%) and β-pinene (5.3%) were major compounds in the oil of sample A. The main constituents of the oil of sample B were β-pinene (11.3%), α-pinene (10.8%), α- cyperone (7.9%), myrtenol (7.1%) and α-selinene (6.6%).

Introduction

Cyperus rotundus L., (family Cyperaceae), also known as purple nutsedge or nutgrass, is a common perennial weed with slender, scaly creeping rhizomes, bulbous at the base and arising singly from the tubers which are about 1-3 cm long. The tubers are externally blackish in colour and reddish white inside, with a characteristic odour. The stems grow to about 25 cm tall and the leaves are linear, dark green and grooved on the upper surface. Inflorescences are small, with 2-4 bracts, consisting of tiny flowers with a red-brown husk. The nut is three-angled, oblong-ovate, yellow in colour and black when ripe. C. rotundus is indigenous to India, but are now found in tropical, subtropical and temperate regions [1,2].
Cyperus rotundus is a multipurpose plant, widely used in traditional medicine around the world to treat stomach aliments, wounds, boils and blisters [3,4,5,6]. A number of pharmacological and biological activities including anti-Candida, anti-inflammatory, antidiabetic, antidiarrhoeal, cytoprotective, antimutagenic, antimicrobial, antibacterial, antioxidant, cytotoxic and apoptotic, anti-pyretic and analgesic activities have been reported for this plant [7,8,9,10,11,12,13,14,15,16,17]. Previous phytochemical studies on C. rotundus revealed the presence of alkaloids, flavonoids, tannins, starch, glycosides and furochromones, and many novel sesquiterpenoids [9,18,19,20,21,22,23,24,25,26,27,28,29].
The chemical composition of the volatile oils of C. rotundus has been extensively studied and four chemotypes (H-, K-, M- O-types), of the essential oils from different parts of Asia have been reported [14,30,31,32,33,34,35]. The H-type from Japan was found to contain α-cyperone (36.6%), β-selinene (18.5%), cyperol (7.4%) and caryophyllene (6.2%). The M-type from China, Hong Kong, Japan, Taiwan and Vietnam had α-cyperone (30.7%), cyperotundone (19.4%), β-selinene (17.8%), cyperene (7.2%) and cyperol (5.6%). The O-type from Japan, Taiwan, Thailand, Hawaii and the Philippines was characterized by cyperene (30.8%), cyperotundone (13.1%) and β-elemene (5.2%). In addition, the Hawaiian O-type had cyperotundone (25.0%) and cyperene (20.7%) as the major compounds. Finally, the K-type, also from Hawaii, was dominated by cyperene (28.7%), cyperotundone (8.8%), patchoulenyl acetate (8.0%) and sugeonyl acetate (6.9%) [30,31].
The rhizome oils of this plant from different countries also showed compositional differences, suggesting the existence of phytochemical varieties. Cyperene (19.2-30.9%) and α-cyperone (4.5-25.2%) were the most abundant constituents of the oils of Nigerian and Tunisian species, but the concentrations of other main components varied [32,33]. The Brazilian species was found to contain α-cyperone (22.8%) and cyperotundone (12.1%) as the main compounds of the oil [34]. The rhizome oils of C. rotundus from India were reported to have α-copaene (11.4-12.1%), cyperene (8.4-11.7%), valerenal (8.7-9.8%), caryophyllene oxide (7.8-9.7%) and trans-pinocarveol (5.2-7.4%), some of which were absent in the species from other countries [35]. Sonwa and Koenig [36] investigated the essential oil of C. rotundus from Germany, and found the oil to be dominated by cyprotene, α-copaene, cyperene, α-selinene, rotundene, cadalene and nootkatene, among others.
As part of our on-going research on the chemical composition of the essential oils of South African medicinal and aromatic plants from the family Cyperaceae, the present investigation reports on the essential oils from the rhizomes of Cyperus rotundus found in South Africa.

Results and Discussion

Hydrodistillation of the fresh rhizomes of C. rotundus collected from the two different locations yielded 0.20% and 0.16% pale yellowish oils for Empangeni (sample A) and KwaDlangezwa (sample B), respectively. The compositions of the two oils are displayed in Table 1, where constituents are listed in order of their elution on the (DB-5) column. A total 58 components were detected, 41 and 43 of which were identified, accounting for 88.9% and 92.0% of the oil of Empangeni and KwaDlangezwa samples, respectively. The oil of the Empangeni sample was characterized by larger amounts of sesquiterpenes (59.8%) than monoterperenes (29.1%), while the KwaDlangezwa oil sample had a relatively similar content of sesquiterpenes (45.9%) and monoterperenes (46.1%). The sesquiterpenic composition of the oil of Empangeni is dominated by α-cyperone (11.0%), caryophyllene oxide (5.4%) and β-selinene (5.1%), and the compounds: myrtenol (7.9%), β-pinene (5.3%) and trans-pinocarveol (4.0%) were the major representative of monoterpenoids. In the oil of KwaDlangezwa, β-pinene (11.3%), α-pinene (10.8%), α-cyperone (7.9%), myrtenol (7.1%), α-selinene (6.6%), limonene (5.7%) and β-selinene (4.6%) were the main constituents. Although the compositions of the oil from each location varied, certain similarities were evident. The composition profile of the oil of Empangeni sample shows that it is richer in sesquiterpenes and is therefore, similar to other reported essential oils compositions of C. rotundus from different countries [14,30,31,32,33,34,35]. However, the chemical pattern of the oil of KwaDlangezwa sample is completely different from the sesquiterpenoid dominating types.
Table
Table 1. Percentage composition of C. rotundus essential oils from two different locations.
Table 1. Percentage composition of C. rotundus essential oils from two different locations.
CompoundsRIaSample ASample BMethod of Identification
α-pinene9363.010.8MS, RI
camphene951t1.5MS, RI
β-pinene9795.311.3MS, RI
myrcene9900.5-MS, RI
α-phellandrene1002t-MS, RI
bicyclo [3.2.0] hept-6-ene *1014-0.3RI
p-cymene1026 1.70.6MS, RI
limonene10302.05.7MS, RI
1,8-cineole1033t-MS, RI
terpinolene10820.6-MS, RI
perillene1096-0.3MS, RI
3,3,5-trimethyl cyclohexene 1118-0.2RI
fenchol 1121-0.2MS, RI
trans-pinocarveol11424.04.0MS, RI
camphene hydrate1147-0.4MS, RI
pinocarvone11582.20.4MS, RI
p-mentha-1, 5-diene-8-ol11610.4-MS, RI
borneol1167-0.3MS, RI
terpinen-4-ol1178 0.91.0MS, RI
myrtenol1201 7.97.1MS, RI
verbenone12090.61.1MS, RI
trans-carveol1226t0.4MS, RI
cuminaldehyde1251t0.1MS, RI
carvone1257-0.2MS, RI
α-copaene1379-0.5MS, RI
β-elemene13830.80.5MS, RI
cyperene13971.62.6MS, RI
β-caryophyllene14260.80.6MS, RI
α-gurjunene1431-0.3MS, RI
α-humulene14520.40.2MS, RI
allo-aromadendrene14681.20.8MS, RI
eudesma-2,4,11-triene 14762.1-MS, RI
β-selinene14845.14.6MS, RI
α-selinene14912.76.6MS, RI
germacrene B 1546-2.1MS, RI
spathulenol15720.3-MS, RI
caryophyllene oxide15845.42.6MS, RI
(2R,5E)-caryophyll-5-en-12-al15931.0-MS, RI
humulene epoxide II16012.71.6MS, RI
oplopenone16083.4-RI
globulol1623-0.9MS, RI
patchenol ** 16283.90.9RI
2-cyclopropylthiophene1631-2.5RI
caryophylla-3,8(13)-dien-5-β-ol16414.22.4RI
vulgarol B16423.81.8RI
caryophylla-3,8(13)-dien-5-α-ol16492.1-RI
caryophyllenol 1116614.80.9RI
aromadendrene epoxide1743-2.7RI
aristolone17522.51.6MS, RI
α-cyperone177111.07.9MS, RI
oxo-α-ylangene1779-1.9MS, RI
M+ 218 (C15H22O) **17831.4-RI
M+ 218 (C15H22O) ** 17961.71.1RI
solavetivone1816t-MS, RI
nootkatone1820-0.2MS, RI
hexadecanoic acid1942t-MS, RI
phytol 2096 t-MS, RI
Monoterpene hydrocarbons 13.130.4
Oxygenated monoterpenes 16.015.5
Sesquiterpene hydrocarbons 14.718.2
Oxygenated sesquiterpenes 45.127.9
Total identified 88.992.0
unidentified 3.11.1
RIa = Retention Indices relative to C9-C24 n-alkanes on the DB-5 column; t = trace amount (≤0.05%); Empangeni = Sample A; KwaDlangezwa = Sample B; * Correct isomer not identified; ** An alcohol of methanoazulene; *** tentatively identified: RIa = 1783: [M+.] 218(100), 41(98), 91(93), 55(70), 79(69), 105(68), 67(63), 203(60); RIa = 1796: [M+.] 95(100), 69(52), 179(50), 41(42), 133(38), 79(28), 107(25), 218(22).
In all the previous reports on the chemical composition of essential oils of C. rotundus from around the world, α-cyperone, cyperene, cyperotundone and β-selinene were found to be the major compounds identified in higher concentrations, along with other constituents such as, α-copaene, valerenal, caryophyllene oxide, patchoulenyl acetate and sugeonyl acetate (Table 2). In addition, some reports have had the occurrence of α-pinene, β-pinene, limonene and 1,8-cineole as minor components of the essential oils of C. rotundus [14,15]. However, in our results (Table 1 and Table 2), cyperene, α-cyperone and β-selinene were present only in small concentrations, while cyperotundone, valerenal, petchoulenyl and sugeonyl acetates were not detected at all in our oil samples. Interestingly, the major constituents of the KwaDlangezwa oil sample, α-pinene (10.8%) and β-pinene (11.3%) have not been previously reported in dominant quantities among the major compounds from the oils of C. rotundus (Table 2).
Table
Table 2. Comparison of major constituents of C. rotundus rhizomes oils from different countries.
Table 2. Comparison of major constituents of C. rotundus rhizomes oils from different countries.
Oil percentage composition
Constituents12345678910
α-pinene---------(3.0-10.8)
β-pinene---------(5.3-11.3)
α-copaene------(11.4-12.1)---
cyperene-30.87.228.720.7-(8.4-11.1)19.2(20.4-30.9)(1.6-2.6)
β-selinene18.3-17.8------(4.6-5.1)
cyperotundone-13.119.48.825.012.1---8.8-
α-cyperone38.6-30.7--22.8-17.7(4.5 – 25.2)(7.9-11.0)
valerenal------(8.7-9.8)---
caryophyllene oxide------(7.8-9.7)--(2.6-5.4)
petchoulenyl acetate---8.0------
sugeonyl acetate---8.9------
1 = H-type; 2 = O-type; 3 = M-type; 4 = K-type; 5 = Hawaiian O-type (Asia countries) [31,32]; 6 = Brazil [35]; 7 = India [36]; 8 = Nigeria [33]; 9 = Tunisia [14,34], 10 = South Africa (present study).
Comparing the present results with those previously reported in the literature on the essential oil compositions of C. rotundus from different countries [14,30,31,32,33,34,35], it is apparent that, there are many differences regarding the major constituents of the oils of C. rotundus, which further suggests the existence of more chemical diversity within the C. rotundus species [33]. However, cyperene and α-cyperone are the two major compounds similar in the essential oils of C. rotundus from Africa (Nigeria, Tunisia and South Africa). The observed compositional difference between C. rotundus found in South Africa and the rest of the world could be due to climactic and environmental conditions, chemotypes, nutritional status of the plants, and other factors, which can influence essential oil composition [14,30,31,37,38,39,40,41]. In conclusion, the essential oils of C. rotundus from South Africa can be related to the M-type due to the presence of cyperene, β-selinene and α-cyperone.

Experimental

Plant material

Fresh plant materials of C. rotundus growing wild on the campus of University of Zululand, KwaDlangezwa (Sample B) and along Empangeni road (Sample A) in the KwaZulu-Natal Province of South Africa were randomly collected in March, 2007. Mrs. N.R Ntuli of Department of Botany, University of Zululand, identified the plant materials. Voucher specimens [Lawal, OA 05 and 06 (ZULU)] were deposited at the University of Zululand Herbarium.

Oil isolation

Finely chopped fresh rhizomes of each sample (500 g) were separately subjected to hydrodistillation in a Clevenger-type apparatus for 4 h in accordance with the British Pharmacopoeia specification [42]. The sample was added to distilled deionized water (1.5 L) in a 2-5 L round-bottomed flask and heated to boiling, after which the essential oil was evaporated together with water vapour and finally collected in a condenser. The upper phase that contained the essential oil was separated from the lower one and the distillate isolated was preserved in a sealed sample tube and stored under refrigeration until analysis [43,44].

GC analyses

GC analysis was carried out on a Hewlett Packard HP 6820 Gas Chromatograph equipped with a FID detector and DB-5 column (60 m x 0.25 mm id), film thickness was 0.25 µm and the split ratio was 1:25. The oven temperature was programmed from 50 °C (after 2 min) to 240 °C at 5 °C/min and the final temperature was held for 10 min. Injection and detector temperatures were 200 °C and 240 °C, respectively. Hydrogen was the carrier gas. An aliquot (0.5 µL of the diluted oil) was injected into the GC. Peaks were measured by electronic integration. A homologous series of n-alkanes were run under the same conditions for determination of retention indices.

GC-M S analyses

GC-MS analyses of the oils were performed on a Hewlett Packard HP 6890 Gas Chromatography interfaced with Hewlett Packard 5973 mass spectrometer system equipped with a DB-5 capillary column (30 m x 0.25 mm id, film thickness 0.25 µm). The oven temperature was programmed from 70- 240 °C at the rate of 5 °C/min. The ion source was set at 240 °C and electron ionization at 70 eV. Helium was used as the carrier gas at a flow rate of 1 mL/min. Scanning range was 35 to 425 amu. 1.0 µL of diluted oil in hexane was injected into the GC/MS.

Identification of components

The components of the oils were identified base on the comparison of their retention indices and mass spectra with those standards, Wiley 275 library mass spectra databased of the GC/MS system and published data [45,46,47].

Acknowledgements

The authors would like to acknowledge the financial support by NRF, South Africa, and University of Zululand Research committee. Mrs. N. R. Ntuli for her help in identifying the plant materials. OAL is grateful to Lagos State University, Ojo, Nigeria for granting training leave.

References

  1. Pooley, E. A Field Guide to Wild Flowers in KwaZulu-Natal and Eastern Region; Natal Flora Publications Trust: Durban, South Africa, 1998; p. 562. [Google Scholar]
  2. Gordon-Gray, K.D. Cyperaceae in Natal; National Botanical Institute: Pretoria, South Africa, 1995; pp. 45–76. [Google Scholar]
  3. Oliver-Bever, B. Medicinal Plants in Tropical West Africa; Cambridge University Press: Cambridge, UK, 1986; p. 200. [Google Scholar]
  4. Puratuchikody, A.; Nithya, D.C.; Nagalakshmi, G. Wound Healing Activity of Cyperus rotundus Linn. Indian J. Pharm. Sci. 2006, 68, 97–101. [Google Scholar] [CrossRef]
  5. Joshi, A.R.; Joshi, K. Indigenous knowledge and uses of medicinal plants by local communities of the Kali Gandaki Watershed Area, Nepal. J. Ethnopharmacol. 2000, 73, 175–183. [Google Scholar] [CrossRef]
  6. El-Kamali, H.H.; El-Khalifa, K.F. Folk medicinal plants of riverside forests of the Southern Blue Nile district, Sudan. Fitoterapia 1999, 70, 493–497. [Google Scholar] [CrossRef]
  7. Durate, M.C.T.; Figueira, G.M.; Sartoratto, A.; Rehder, V.L.G.; Delarmelina, C. Anti-Candida activity of Brazilian medicinal plant. J. Ethnopharmacol. 2005, 97, 305–311. [Google Scholar] [CrossRef]
  8. Sundaram, M.S.; Sivakumar, T.; Balamurugan, G. Anti-inflammatory effect of Cyperus rotundus Linn. Leaves on acute and subacute inflammation in experimental rat models. Biomedicine 2008, 28, 302–304. [Google Scholar]
  9. Raut, N.A.; Gaikwad, N.J. Antidiabetic activity of hydro-ethanolic extract of Cyperus rotundus in alloxan induced diabetes in rats. Fitoterapia 2006, 77, 585–588. [Google Scholar] [CrossRef]
  10. Uddin, S.J.; Mondal, K.; Shilpi, J.A.; Rahman, M.T. Antidiarrhoeal activity of Cyperus rotundus. Fitoterapia 2006, 77, 134–136. [Google Scholar] [CrossRef]
  11. Kilani, S.; Ben Ammar, R.; Bouhlel, I.; Abdelwahed, A.; Hayder, N.; Mahmoud, A.; Ghedira, K.; Chekir-Ghedira, L. Investigation of extracts from (Tunisian) Cyperus rotundus as antimutagens and radical scavengers. Environ. Toxicol. Pharmacol. 2005, 20, 478–484. [Google Scholar] [CrossRef]
  12. Zhu, M.; Luk, H.H.; Fung, H.S.; Luk, C.T. Cytoprotective effects of Cyperus rotundus against ethanol induced gastric ulceration in rats. Phytother. Res. 1997, 11, 392–394. [Google Scholar] [CrossRef]
  13. Kilani, S.; Bouhlel, I.; Ben Ammar, R.; Ben Sghair, M.; Skandrani, I.; Boubaker, J.; Mahmoud, A.; Dijoux-Franca, M.G.; Ghedira, K.; Chekir-Ghedira, L. Chemical investigation of different extracts and essential oil from the tubers of (Tunisian) Cyperus rotundus. Correlation with their antiradical and antimutagenic properties. Ann. Microbiol. 2007, 57, 657–664. [Google Scholar] [CrossRef]
  14. Kilani, S.; Ledauphin, J.; Bouhlel, I.; Ben Sghaier, M.; Boubaker, J.; Skandrani, I.; Mosrati, R.; Ghedira, K.; Barillier, D.; Chekir-Ghedira, L. Comparative study of Cyperus rotundus essential oil by a modified GC/MS analysis method. Evaluation of its antioxidant, cytotoxic, and apoptotic effects. Chem. Biodivers. 2008, 5, 729–742. [Google Scholar] [CrossRef]
  15. Dhillon, R.S.; Singh, S.; Kundra, S.; Basra, A.S. Studies on the chemical composition and biological activity of essential oil from Cyperus rotundus Linn. Plant Growth Regul. 1993, 13, 89–93. [Google Scholar]
  16. Pal, D.K.; Dutta, S. Evaluation of the Antioxidant activity of the roots and Rhizomes of Cyperus rotundus L. Indian J. Pharm. Sci. 2006, 68, 256–258. [Google Scholar] [CrossRef]
  17. Kilani, S.; Ben Sghaier, M.; Limem, I.; Bouhlel, I.; Boubaker, J.; Bhouri, W.; Skandrani, I.; Neffatti, A.; Ben Ammar, R.; Dijoux-Franca, M.G.; Ghedira, K.; Chekir-Ghedira, L. In vitro evaluation of antibacterial, antioxidant, cytotoxic and apoptotic activities of the tubers infusion and extracts of Cyperus rotundus. Bioresour. Technol. 2008, 99, 9004–9008. [Google Scholar] [CrossRef]
  18. EL-Habashy, I.; Mansour, R.M.A.; Zahran, M.A.; EL-Hadid, M.M.; Saleh, N.A. Leaf flavonoids of Cyperus species in Egypt. Bio. Syst. Ecol. 1989, 17, 191–195. [Google Scholar]
  19. Harborne, JB.; Williams, C.A.; Wilson, K.L. Flavonoids in leaves and inflorescences of Australian Cyperus species. Phytochemistry 1982, 21, 2491–2507. [Google Scholar] [CrossRef]
  20. Umerie, S.C.; Ezeuzo, H.O. Physicochemical characterization and utilization of Cyperus rotundus starch. Bioresour. Technol. 2000, 72, 193–196. [Google Scholar] [CrossRef]
  21. Sayed, H.M.; Mohamed, M.H.; Farag, S.F.; Mohamed, G.A.; Proksch, P. A new steroid glycoside and furochromones from Cyperus rotundus L. Nat. Prod. Res. 2007, 21, 343–350. [Google Scholar] [CrossRef]
  22. Kapadia, V.H.; Naik, V.G.; Wadia, M.S.; Dev, S. Sesquiterpenoids from Essential oil of Cyperus rotundus. Tetrahedron Lett. 1967, 4661. [Google Scholar] [CrossRef]
  23. Trivedi, B.; Motl, O.; Herout, V.; Sorm, F. Composition of the oil from Cyperus rotundus: Structure of patchoulenone. Coil. Czech. Chem. Commun. 1984, 29, 1675–1688. [Google Scholar] [CrossRef]
  24. Thebtaranonth, C.; Thebtaranonth, Y.; Wanauppaphamkul, C.; Yuthavong, Y. Antimalarial sesquiterpenes from tubers of Cyperus rotundus. Structure of 10, 12-peroxycalamenene, Asesquiterpenes endoperoxide. Phytochemistry 1995, 40, 125–128. [Google Scholar] [CrossRef]
  25. Ohira, S.; Hasegawa, T.; Hyashi, K.I.; Hoshino, T.; Takaoka, D.; Nozaki, H. Sesquiterpenoids from Cyperus rotundus. Phytochemistry 1998, 47, 1577–1581. [Google Scholar] [CrossRef]
  26. Jeong, S.J.; Miyamoto, T.; Inagaki, M.; Kim, Y.C.; Higuchi, R. Rotundines A-C, three novel sesquiterpene alkaloids from Cyperus rotundus. J. Nat. Prod. 2000, 63, 673–675. [Google Scholar] [CrossRef]
  27. Kilani, S.; Ben Ammar, R.; Bouhlel, I.; Abdelwahed, A.; Hayder, N.; Mahmoud, A.; Ghedir, K.; Chekir-Ghedira, L. Investigation of extracts from (Tunisian) Cyperus rotundus as antimutagens and radical scavengers. Environ. Toxicol. Pharmacol. 2005, 20, 478–484. [Google Scholar] [CrossRef]
  28. Morimoto, M.; Komai, K. Plant growth inhibitors: Patchoulane-type sesquiterpenes from Cyperus rotundus L. Weed Biol. Manag. 2005, 5, 203–209. [Google Scholar] [CrossRef]
  29. Xu, Y.; Zhang, H.; Yu, C.; Lu, Y.; Chang, Y.; Zou, Z. Norcyperone, a Novel Skeleton Norsesquiterpene from Cyperus rotundus L. Molecules 2008, 13, 2474–2481. [Google Scholar] [CrossRef]
  30. Komai, K.; Tang, C. A Chemotype of Cyperus rotundus in Hawaii. Phytochemistry 1989, 28, 1883–1886. [Google Scholar] [CrossRef]
  31. Komai, K.; Shimizu, M.; Tang, C.T.; Tsutsui, H. Sesquiterpenoids of Cyperus bulbosus, Cyperus tuberosus and Cyperus rotundus. Mem. Fac. Agr. Kinki Univ. 1994, 27, 39–45. [Google Scholar]
  32. Ekundayo, O.; Oderinde, R.; Ogundeyin., M.; Biskup, E.S. Essential oil constituents of Cyperus tuberosus Rottb. Rhizomes. Flav. Fragr. J. 1991, 6, 261–264. [Google Scholar] [CrossRef]
  33. Kilani, S.; Abdelwahed, A.; Chraief, I.; Ben Ammar, R.; Hayder, N.; Hammami, M.; Ghedira, K.; Chekir-Ghedira, L. Chemical composition, antibacterial and antimutagenic activities of essential oil from (Tunisian) Cyperus rotundus. J. Essent. Oil Res. 2005, 17, 695–700. [Google Scholar] [CrossRef]
  34. Zoghbi, M.D.G.B.; Andrade, E.H.A.; Carreira, L.M.M.; Rocha, E.A.S. Comparison of the main components of the essential oils of "priprioca": Cyperus articulatus var. articulatus L., C. articulatus var. nodosus L., C. prolixus Kunth and C. rotundus L. J. Essent. Oil Res. 2008, 20, 42–46. [Google Scholar] [CrossRef]
  35. Jirovetz, L.; Wobus, A.; Buchbauer, G.; Shafi, M.P.; Thampi, P.T. Comparative analysis of the essential oil and SPME-headspace aroma compounds of Cyperus rotundus L. roots/tubers from South-India using GC, GC-MS and olfactometry. J. Essent. Oil-Bearing Plants 2004, 7, 100–106. [Google Scholar] [CrossRef]
  36. Sonwa, M.M.; Koenig, W.A. Chemical study of essential oil Cyperus rotundus. Phytochemistry 2001, 58, 799–810. [Google Scholar] [CrossRef]
  37. Loziene, K.; Venskutonis, P.R. Influence of environmental and genetic factors on the stability of essential oil composition of Thymus pulegioides. Bio. Syst. Ecol. 2005, 33, 517–525. [Google Scholar] [CrossRef]
  38. Clark, R.J.; Menary, R.C. Environmental effects on peppermint (Mentha piperita L.). II. Effects of temperature on photosynthesis, photorespiration and dark respiration in peppermint with reference to oil composition. Aust. J. Plant Physiol. 1980, 7, 693–697. [Google Scholar] [CrossRef]
  39. Boira, H.; Blanquer, A. Environmental factors affecting chemical variability of essential oils in Thymus piperella L. Bio. Syst. Ecol. 1998, 26, 811–822. [Google Scholar] [CrossRef]
  40. Burbott, A.J.; Loomis, W.D. Effects of light and temperature on the monoterpenes of peppermint. Plant Physiol. 1967, 42, 20–28. [Google Scholar] [CrossRef]
  41. Lahlou, M. Methods to study the phytochemistry and bioactivity of essential oils. Phytother. Res. 2004, 18, 435–448. [Google Scholar] [CrossRef]
  42. British Pharmacopoeia Part II; HMSO: London, 1988; pp. 109–110.
  43. Oyedeji, O.A.; Ekundayo, O.; Koenig, W.A. Constituents of the essential oils from the leaves of Lenontis nepetaefolia (L.) Ait. F. J. Essent. Oil Res. 1999, 11, 716–718. [Google Scholar] [CrossRef]
  44. Oyedeji, O.A.; Ekundayo, O.; Olawore, O.N.; Koenig, W.A. Essential oil composition of two varieties of Eucalyptus camaldulensis from Nigeria. J. Essent. Oil Res. 2000, 12, 102–104. [Google Scholar] [CrossRef]
  45. Adams, R.P. Identification of Essential Oil Components by Ion Trap Mass Spectroscopy; Academic Press: New York, NY, USA, 1989. [Google Scholar]
  46. Joulain, D.; Koenig., W.A. The Atlas of Spectral Data of Sesquiterpenes Hydrocarbons; E.B-Verlag: Hamburg, Germany, 1998. [Google Scholar]
  47. ESO 2000. The complete database of essential oils, Boelens Aroma Chemical Information Service: The Netherlands, 1999.
  • Sample Availability: Samples of the oils and the spectra are available on request from the authors.

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MDPI and ACS Style

Lawal, O.A.; Oyedeji, A.O. Chemical Composition of the Essential Oils of Cyperus rotundus L. from South Africa. Molecules 2009, 14, 2909-2917. https://doi.org/10.3390/molecules14082909

AMA Style

Lawal OA, Oyedeji AO. Chemical Composition of the Essential Oils of Cyperus rotundus L. from South Africa. Molecules. 2009; 14(8):2909-2917. https://doi.org/10.3390/molecules14082909

Chicago/Turabian Style

Lawal, Oladipupo A., and Adebola O. Oyedeji. 2009. "Chemical Composition of the Essential Oils of Cyperus rotundus L. from South Africa" Molecules 14, no. 8: 2909-2917. https://doi.org/10.3390/molecules14082909

APA Style

Lawal, O. A., & Oyedeji, A. O. (2009). Chemical Composition of the Essential Oils of Cyperus rotundus L. from South Africa. Molecules, 14(8), 2909-2917. https://doi.org/10.3390/molecules14082909

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