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Article

One-Flask Synthesis of Pyrazolo[3,4-d]pyrimidines from 5-Aminopyrazoles and Mechanistic Study

by
Wan-Ping Yen
1,2,
Shuo-En Tsai
1,2,
Naoto Uramaru
3,
Hiroyuki Takayama
4 and
Fung Fuh Wong
1,*
1
School of Pharmacy, China Medical University, No. 91 Hsueh-Shih Rd., Taichung 40402, Taiwan
2
Program for Biotech Pharmaceutical Industry, China Medical University, No. 91 Hsueh-Shih Rd., Taichung 40402, Taiwan
3
Department of Environmental Science, Nihon Pharmaceutical University, Komuro Inamachi Kita-adachi-gun, Saitama-ken 10281, Japan
4
Department of Medico Pharmaceutical Science, Nihon Pharmaceutical University, Komuro Inamachi Kita-adachi-gun, Saitama-ken 10281, Japan
*
Author to whom correspondence should be addressed.
Molecules 2017, 22(5), 820; https://doi.org/10.3390/molecules22050820
Submission received: 18 March 2017 / Revised: 11 May 2017 / Accepted: 11 May 2017 / Published: 16 May 2017
(This article belongs to the Collection Heterocyclic Compounds)
Browse Figures
Versions Notes

Abstract

:
A novel one-flask synthetic method was developed in which 5-aminopyrazoles were reacted with N,N-substituted amides in the presence of PBr3. Hexamethyldisilazane was then added to perform heterocyclization to produce the corresponding pyrazolo[3,4-d]pyrimidines in suitable yields. These one-flask reactions thus involved Vilsmeier amidination, imination reactions, and the sequential intermolecular heterocyclization. To study the reaction mechanism, a series of 4-formyl-1,3-diphenyl-1H-pyrazol-5-yl-N,N-disubstituted formamidines, which were conceived as the chemical equivalent of 4-(iminomethyl)-1,3-diphenyl-1H-pyrazol-5-yl-formamidine, were prepared and successfully converted into pyrazolo[3,4-d]pyrimidines. The experiments demonstrated that the reaction intermediates were the chemical equivalents of 4-(iminomethyl)-1,3-diphenyl-1H-pyrazol-5-yl)formamidines. The rate of the reaction could be described as being proportional to the reactivity of amine reactants during intermolecular heterocyclization, especially when hexamethyldisilazane was used.

Graphical Abstract

1. Introduction

One-flask reactions possess significant advantages and have emerged as a powerful tool in synthetic organic chemistry and reaction design approaches [1,2,3]. The main advantages of using one-flask reactions in organic syntheses are their green chemistry nature and high atom economy due to the lack of workup or the isolation of intermediates involved [4,5,6,7,8,9,10,11,12]. We previously reported an efficient one-pot three-component synthesis of pyrazolo[3,4-d]pyrimidines that involved treatment of 5-aminopyrazoles with formamide using PBr3 as the coupling agent (Scheme 1) [13,14,15].
Pyrazolopyrimidine derivatives are important structural moieties, found in pharmacologically active compounds such as a novel series of glucokinase activators [16], antibacterial [17], antifungal [18,19], antioxidant [20], antitumor [21,22,23,24], herbicidal [25], antivirus [26,27], anticancer compounds [28,29], and effective inhibitors of inflammatory mediators in intact cells [30,31]. The pyrazolo[3,4-d]pyrimidine core is also isomeric with the biologically significant purine system [32,33]. Numerous synthetic methods were developed for preparing pyrazolopyrimidine derivatives [34,35,36,37]. However, most of these methods are not straightforward and their purification steps are troublesome. Therefore, new and convenient routes for the synthesis of pyrazolo[3,4-d]pyrimidine systems have attracted considerable attention [13,14,15].
In this study, we extended our previous one-pot three-component approach for the synthesis of a series of pyrazolo[3,4-d]pyrimidine derivatives to develop a novel one-flask synthesis involving Vilsmeier amidination, imination reactions, and sequential intermolecular heterocyclization. First, 5-aminopyrazoles were treated with various Vilsmeier agents, which were generated from the corresponding amide solvents, including N,N-dimethylformamide (DMF), N,N-diethylformamide (DEF), N,N-diisopropylformamide, N,N-di-n-butylformamide, piperidine-1-carbaldehyde, and pyrrolidine-1-carbaldehyde in the presence of tribromophosphine PBr3, to produce the corresponding 4-(iminomethyl)-1,3-diphenyl-1H-pyrazol-5-yl-N,N-disubstituted formamidine intermediates (Scheme 1) [38,39]. Without isolating the intermediates, we sequentially evaluated the intermolecular heterocyclization reactivity between 4-(iminomethyl)-1,3-diphenyl-1H-pyrazol-5-yl-formamidines and amines such as hexamethyldisilazane, hexamethylenetetramine, lithium bis(trimethylsilyl)amine, and sodium bis(trimethylsilyl)amine. These experimental results revealed that commercially available N,N-dimethylformamide (DMF)/PBr3 and hexamethyldisilazane were the optimal Vilsmeier agent and the promotor, respectively. Specifically, we successfully combined the Vilsmeier amidination and imination reactions with intermolecular heterocyclization to design a high-efficiency one-flask synthesis for the preparation of a series of pyrazolo[3,4-d]pyrimidines.

2. Results and Discussion

To optimize the one-flask process for the synthesis of pyrazolo[3,4-d]pyrimidine derivatives 3a–n via the sequential Vilsmeier reaction and intermolecular heterocyclization and explain the mechanism the study illustrated in Scheme 2 was performed. 5-Amino-1,3-diphenylpyrazole (1a) was prepared by our previously developed method [38,39] and used as the model starting material to improve the intermolecular heterocyclization reaction conditions. Following the reliable published procedure for the Vilsmeier reaction 5-aminopyrazole 1a was treated with 3.0 equivalent of PBr3 in N,N-dimethylformamide (DMF) solution at 60 °C for 1.0–2.0 h. The corresponding 4-(iminomethyl)-1,3-diphenyl-1H-pyrazol-5-yl-formamidine 2a was thus obtained in excellent yield (>90%).
Without isolation of intermediate 2a, various amines, including hexamethyldisilazane (NH(SiMe3)2), hexamethylenetetramine, lithium bis(trimethylsilyl)amine (LiN(SiMe3)2), and sodium bis(trimethylsilyl)amine (NaN(SiMe3)2) were added into the reaction mixture and the solution was heated at reflux for 3–5 h to establish the best heterocyclization conditions (see Table 1). Without the amine agent, only 4-formyl-1,3-diphenyl-1H-pyrazol-5-yl-formamidine 7a, which is the chemical equivalent of 4-(iminomethyl)-1,3-diphenyl-1H-pyrazol-5-yl-formamidine intermediate 2a, was isolated after work-up and purification (see entry 1 in Table 1). Among the amines, the corresponding pyrazolo[3,4-d]pyrimidine product 3a can be produced and isolated in yields ranging from 26% to 91%. Based on the results, we found that commercially available hexamethyldisilazane (NH(SiMe3)2) provided the best result (91% yield) and the reactivity tendency of the amines was NH(SiMe3)2 > NaN(SiMe3)2 > LiN(SiMe3)2 > hexamethylenetetramine (see the Entries 2–5 in Table 1). We next tried different amounts of NH(SiMe3)2, including 1.0, 2.0, 3.0, and 4.0 equivalents. The corresponding pyrazolo[3,4-d]pyrimidine product 1a was obtained in 56–91% yield, with the best yield (91%) corresponding to 3 equivalents of (NH(SiMe3)2) (see the Entries 5 and 6–8 in Table 1). Consequently, we believe that 3.0 equivalent of NH(SiMe3)2 is the optimum amount for our reaction conditions.
To determine the reactivity of the different Vilsmeier agents (HC(O)NR1R2 + PBr3), we used different amide solvents, including N,N-dimethylformamide (DMF), N,N-diethylformamide (DEF), N,N-diisopropylformamide, N,N-di-n-butylformamide, piperidine-1-carbaldehyde, and pyrrolidine-1-carbaldehyde in the presence of 3.0 equivalent of PBr3 to prepare the corresponding types of Vilsmeier reagent. Compound 1a was allowed to react sequentially with these different Vilsmeier reagents at 60 °C for 1.0–2.0 h. When the starting material 1a was fully consumed, 3.0 equivalents of NH (SiMe3)2 were added to the reaction mixture which was heated at reflux for 3.0–5.0 h. After the work-up and purification, the corresponding pyrazolo[3,4-d]pyrimidine 3a was obtained in 56–91% yields (see Table 2). Based on the study, commercially available DMF was the best solvent for the preparation of the Vilsmeier reagent in this new one-flask procedure. Based on our optimized experimental results, we believe the most reliable procedure for the one-flask synthesis of pyrazole[3,4-d]pyrimidines involves the treatment of 5-aminopyrazole 1a with 3.0 equivalent of PBr3 in DMF solution at 60 °C for 1.0–2.0 h. When the Vilsmeier reaction was completed, the resulting mixture was added with 3.0 equivalents of NH(SiMe3)2 then heated at reflux at 70 °C to 80 °C for 3.0–5.0 h (monitored by TLC). After work-up and purification by chromatography, the corresponding pyrazolo[3,4-d]pyrimidine 3a was obtained in excellent yield (91%, see Table 2).
Application of the optimized one-flask inter-heterocyclization procedure to 5-amino-1,3-disubstituted pyrazoles 1b–i bearing various N1 substituents, including o-Me-Ph, o-Cl-Ph, m-Me-Ph, m-Cl-Ph, m-NO2-Ph, p-Me-Ph, p-Cl-Ph, and p-Br-Ph, also proceeded smoothly to give the corresponding pyrazolo[3,4-d]pyrimidines 3a–i in 78–91% yields (see Table 3).
For further investigation of the effect of the substituent on the C-3 of the pyrazole ring, the same conditions were employed with 5-amino-1-phenyl-3-substituted pyrazoles 1j–n that contained methyl, t-butyl, p-Me-Ph, p-Cl-Ph, or p-OMe-Ph groups at the C-3 position of the pyrazole ring. The reaction also proceeded smoothly gave the corresponding products 3j–n in 79–91% yields (see Table 3). All pyrazolo[3,4-d]pyrimidines 3a–n were fully characterized by spectroscopic methods and the physical properties and spectroscopic characteristics of the pyrazolo[3,4-d]pyrimidines 3a–n were consistent with our published data [13,14,15].
For further comparison of the reactivity between this new intermolecular Vilsmeier heterocyclization and the previously published intramolecular heterocyclization method [13,14,15], 5-aminopyrazoles were treated with formamide/PBr3. Based on the results of Table 3, the corresponding pyrazolopyrimidines 3a–n were obtained in 78–91% yields by the intermolecular heterocyclization route and in 87–96% yields by intramolecular heterocyclization, respectively. The data suggests that the intramolecular heterocyclization is more favorable as it provided the better isolated yields.
We propose a plausible mechanism for the newly developed one-flask cascade for synthesis of pyrazolo [3,4-d]pyrimidines as shown in Scheme 3. Initiallly, N,N-dimethylformamide (DMF) reacted with the coupling agent PBr3 to form the Vilsmeier reactive species 4 in situ [40,41,42,43,44]. Sequentially, 5-amino-1,3-disubstituted pyrazoles 1a–n reacted with the reactive species 4 to undergo the amidination and imination reaction to give the 1H-pyrazol-5-yl-N,N-disubstituted formamidine intermediates 2a–n (see Scheme 3). When the Vilsmeier reaction was complete (by monitoring TLC), NH(SiMe3)2 was directly added into the reaction mixture to perform the substitution reaction with the imino group to generate intermediate 5. A sequential intermolecular heterocyclization reaction then took place to produce intermediate 6. After the desilylation reaction occurred caused by bromide anion and water, the corresponding pyrazolo[3,4-d]pyrimidines 3a–n were obtained in good yields.
To further study the mechanism, 4-formyl-1,3-diphenyl-1H-pyrazol-5-yl-N,N-dimethyl formamidine 7a was synthesized [20] and reacted with various amines including NH(SiMe3)2, hexamethylenetetramine, LiN(SiMe3)2, and NaN(SiMe3)2, to carry out the intermolecular heterocyclization. The heterocyclization was successfully and smoothly underwent to give pyrazole[3,4-d]pyrimidine product 3a in 37–91% yields. Particularly, NH(SiMe3)2 was most efficient base for heterocyclization to afford the desired product in 91% yield (see Entry 4 in Table 4). The similar reactivity tendency of heterocyclization was observed in this study: NH(SiMe3)2 > NaN(SiMe3)2 > LiN(SiMe3)2 > hexamethylenetetramine (see Entries 1–4 in Table 4). 4-formyl-1,3-diphenyl-1H-pyrazol-5-yl-N,N-disubtituted formamidines 7b–e with grafting the different amino-substituent on amidinyl groups, such as NEt2, N(i-Pr)2, N(n-Bu)2, and piperidinyl, were then allowed to reacted with NH(SiMe3)2 in DMF solution at reflux to give the corresponding pyrazolo[3,4-d]pyrimidine 3a for the investigation of the reactivity of substrates. Based on the experimental result, among of starting substrates 7b–e displayed the good to excellent reactivity in heterocyclization, except for 7c possessing the bulky N (i-Pr)2 substituent moiety on amidinyl groups (see Entries 1 and 5–8 in Table 4). Furthermore, 4-formyl-1,3-diphenyl-1H-pyrazol-5-yl-N,N-dimethyl formamidine 7a with the NMe2 substituent on amidinyl groups was the best suitable reactant in the intermolecular heterocyclization (91%, see Table 4). The above results also gave more proof to our proposed mechanism, for example, the new one-flask reaction would take place through 4-(iminomethyl)-1H-pyrazol-5-yl-formamidine intermediates 2a–n. On the other hands, the commercial available N,N-dimethylformamide (DMF) in the presence of PBr3 and hexamethyldisilazane were the best Vilsmeier agent and the promoted cyclization base.

3. Experimental Section

3.1. General Information

All chemicals were reagent grade and used as purchased. All reactions were carried out under nitrogen atmosphere and monitored by TLC analysis. Flash column chromatography purification of compounds was carried out by gradient elution using hexanes in ethyl acetate (EA) unless otherwise stated. Commercially available reagents were used without further purification unless otherwise noted. 1H-NMR were recorded at 200, 400, or 500 MHz and 13C-NMR recorded at 50, 100, or 125 MHz, respectively, in CDCl3, CH3OD, and DMSO-d6 as solvent (see supplementary materials). The standard abbreviations s, d, t, q, and m refer to singlet, doublet, triplet, quartet, and multiplet, respectively. Coupling constant (J), whenever discernible, have been reported in Hz. Infrared spectra (IR) were recorded as neat solutions or solids; and mass spectra were recorded using electron impact or electrospray ionization techniques. The wavenumbers reported are referenced to the polystyrene 1601 cm–1 absorption. High-resolution mass spectra were obtained by means of a JMS-HX110 mass spectrometer (JEOL, Tokyo, Japan).

3.2. Standard Procedure for the Synthesis of Pyrazolo[3,4-d]pyrimidines 3a–n

The optimized procedure involved the treatment of 5-aminopyrazoles 1a–n (1.0 equiv) with PBr3 (~3 equiv.) in various amide solutions including N,N-dimethylformamide (DMF), N,N-diethylformamide (DEF), N,N-diisopropylformamide, N,N-di-n-butylformamide, piperidine-1-carbaldehyde, or pyrrolidine-1-carbaldehyde (2 mL) at 50–60 °C for 1.0–2.0 h. When the reaction was completed (as monitored by TLC), an amine such as hexamethyldisilazane (NH(SiMe3)2), hexamethylenetetramine, lithium bis(trimethylsilyl)amine (LiN(SiMe3)2), or sodium bis(trimethylsilyl)amine (NaN(SiMe3)2) was added into the reaction mixture which was stirred at reflux for 3–5 h. When the intermolecular heterocyclization was complete, the resulting mixture was added to saturated sodium bicarbonate (15 mL) and extracted with dichloromethane (15 mL × 2). The organic extracts were dried over MgSO4, filtered, and concentrated under reduced pressure. The residue was purified by column chromatography on silica gel to give the corresponding pyrazolo[3,4-d]pyrimidines 3a–n in 69–91% yields.
1,3-Diphenyl-1H-pyrazolo[3,4-d]pyrimidine (3a) [13,14,15,45]. Light-yellow solid; m.p. 158–159 °C (hexane–EtOAc). 1H-NMR (CDCl3, 400 MHz): δ 7.34–7.38 (1H, m, ArH), 7.48–7.51 (1H, m, ArH), 7.53–7.57 (4H, m, ArH), 8.06 (2H, d, J = 8.00 Hz, ArH), 8.31 (2H, d, J = 8.00 Hz, ArH), 9.12 (1H, s), 9.51 (1H, s). 13C-NMR (CDCl3, 100 MHz,): δ 114.24, 121.46 (2 × C), 126.86, 127.39 (2 × C), 129.24 (4 × C), 129.64, 131.50, 138.50, 145.00, 152.82, 153.34, 155.61. IR (KBr): 1632, 1586, 1554, 1497, 1366, 1219 cm–1. EIMS m/z: 272 (M+, 100), 273 (18), 271 (31), 142 (11), 77 (34), 69 (24), 51 (11).
1-(2-Methylphenyl)-3-phenyl-1H-pyrazolo[3,4-d]pyrimidine (3b) [13,14,15]. Light-yellow solid; m.p. 140–141 °C (hexane–EtOAc). 1H-NMR (CDCl3, 400 MHz): δ 2.48 (3H, s, CH3), 7.18 (1H, d, J = 7.60 Hz, ArH), 7.42–7.45 (1H, m, ArH), 7.50 (1H, d, J = 8.00 Hz, ArH), 7.54–7.58 (2H, m, ArH), 8.05–8.10 (2H, m, ArH), 9.12 (1H, s), 9.50 (1H, s). 13C-NMR (CDCl3, 100 MHz): δ 21.6 (CH3), 114.18, 118.77, 122.17, 127.42 (2 × C), 127.77, 129.06, 129.23 (2 × C), 129.61, 131.55, 138.38, 139.33, 144.92, 152.80, 153.32, 155.60. IR (KBr): 1636, 1497, 1223, 1096 cm–1. EIMS m/z: 286 (M+, 100), 287 (20), 285 (19), 77 (10).
1-(2-Chlorophenyl)-3-phenyl-1H-pyrazolo[3,4-d]pyrimidine (3c) [13,14,15]. Yellow solid; m.p. 139–140 °C (hexane–EtOAc). 1H-NMR (CDCl3, 400 MHz): δ 7.48–7.51 (3H, m, ArH), 7.53–7.57 (2H, m, ArH), 7.60–7.64 (2H, m, ArH), 8.05 (2H, d, J = 8.00 Hz, ArH), 9.08 (1H, s), 9.54 (1H, s). 13C-NMR (CDCl3, 100 MHz): δ 113.01, 125.54, 127.38 (2 × C), 127.72, 128.38, 129.65 (2 × C), 130.08, 130.82, 131.40, 132.19, 134.74, 145.70, 152.87, 154.50, 155.91. IR (KBr): 3012, 1636, 1582, 1497, 1362, 1223, 1084 cm–1. EIMS m/z: 306 (M+, 96), 308 (28), 307 (M+ + 1, 15), 272 (15), 271 (100), 195 (11), 77 (42), 75 (10), 51 (11).
1-(3-Methylphenyl)-3-phenyl-1H-pyrazolo[3,4-d]pyrimidine (3d) [13,14,15]. Yellow solid; m.p. 80–81 °C (hexane–EtOAc). 1H-NMR (CDCl3, 400 MHz): δ 2.52 (3H, s, CH3), 7.18 (1H, d, J = 8.00 Hz, ArH), 7.41–7.45 (1H, m, ArH), 7.49 (1H, d, J = 7.20 Hz, ArH), 7.53–7.57 (2H, m, ArH), 8.05–8.10 (4H, m, ArH), 9.16 (1H, s), 9.53 (1H, s). 13C-NMR (CDCl3, 100 MHz): δ 21.60, 114.15, 118.70, 122.11, 127.38 (2 × C), 127.72, 129.02, 129.19 (2 × C), 129.57, 131.52, 138.36, 139.28, 144.86, 152.75, 153.27, 155.55. IR (KBr): 1632, 1613, 1585, 1493, 1420, 1366, 1265 cm–1. EIMS m/z: 286 (M+, 100), 287 (22), 285 (21), 77 (9).
1-(3-Chlorophenyl)-3-phenyl-1H-pyrazolo[3,4-d]pyrimidine (3e) [13,14,15]. White solid; m.p. 185–186 °C (hexane–EtOAc). 1H-NMR (CDCl3, 400 MHz): δ 7.31 (1H, d, J = 8.00 Hz, ArH), 7.44–7.50 (2H, m, ArH), 7.52–7.57 (2H, m, ArH), 8.32–8.42 (2H, m, ArH), 9.14 (1H, s), 9.49 (1H, s). 13C-NMR (CDCl3, 100 MHz): δ 114.51, 118.90, 121.07, 126.61, 127.40 (2 × C), 129.25 (2 × C), 129.85, 130.21, 131.17, 134.95, 139.58, 145.62, 152.88, 153.61, 155.73. IR (KBr): 1585, 1555, 1489, 1404, 1366, 1312, 1215, 1088 cm–1. EIMS m/z: 306 (M+, 100), 308 (32), 307 (M+ + 1, 26), 305 (22), 77 (16).
1-(3-Nitrophenyl)-3-phenyl-1H-pyrazolo[3,4-d]pyrimidine (3f) [13,14,15]. Yellow solid; m.p. 179–180 °C (hexane–EtOAc). 1H-NMR (CDCl3, 400 MHz): δ 7.54–7.61 (3H, m, ArH), 7.73 (1H, dd, J = 8.0, 16.4 Hz, ArH), 8.08 (2H, d, J = 8.00 Hz, ArH), 8.19 (1H, d, J = 8.00 Hz, ArH), 8.87 (1H, d, J = 8.00 Hz, ArH), 9.20 (1H, s), 9.37 (1H, s), 9.55 (1H, s). 13C-NMR (CDCl3, 100 MHz): δ 114.74, 115.70, 120.87, 126.04, 127.51 (2 × C), 129.36 (2 × C), 130.16, 130.18, 130.88, 139.64, 146.08, 148.87, 153.14, 154.00, 156.05. IR (KBr): 1636, 1528, 1489, 1346, 1003 cm–1. EIMS m/z: 317 (M+, 100), 318 (17), 77 (14).
1-(4-Methylphenyl)-3-phenyl-1H-pyrazolo[3,4-d]pyrimidine (3g) [13,14,15]. Brown solid; m.p. 133–134 °C (hexane–EtOAc). 1H-NMR (CDCl3, 400 MHz): δ 2.39 (3H, s, CH3), 7.30 (2H, d, J = 8.00 Hz, ArH), 7.45 (1H, d, J = 8.00 Hz, ArH), 7.50 (2H, dd, J = 7.2, 14.8 Hz, ArH), 8.00 (2H, d, J = 8.00 Hz, ArH), 8.11 (2H, d, J = 8.00 Hz, ArH), 9.07 (1H, s), 9.43 (1H, s). 13C-NMR (CDCl3, 100 MHz): δ 20.98, 113.92, 121.21 (2 × C), 127.18 (2 × C), 128.76 (2 × C), 129.37, 129.61 (2 × C), 131.43, 135.94, 136.56, 144.47, 152.58, 152.95, 155.33. IR (KBr): 1636, 1589, 1516, 1386, 1219, 1088 cm–1. EIMS m/z: 286 (M+, 100), 287 (22), 285 (28), 77 (10). HRMS Calcd. for C18H14N4: 286.1218; Found: 286.1216.
1-(4-Chlorophenyl)-3-phenyl-1H-pyrazolo[3,4-d]pyrimidine (3h) [13,14,15]. Yellow solid; m.p. 147–148 °C (hexane–EtOAc). 1H-NMR (CDCl3, 400 MHz): δ 7.48–7.51 (3H, m, ArH), 7.53–7.57 (2H, m, ArH), 8.03 (2H, d, J = 8.00 Hz, ArH), 8.32 (2H, d, J = 8.00 Hz, ArH), 9.11 (1H, s), 9.49 (1H, s). 13C-NMR (CDCl3, 100 MHz): δ 114.32, 122.24 (2 × C), 127.37 (2 × C), 129.23 (2 × C), 129.28 (2 × C), 129.78, 131.24, 132.11, 137.13, 145.23, 152.89, 153.36, 155.66. IR (KBr): 1632, 1555, 1497, 1215, 1054 cm–1. EIMS m/z: 306 (M+, 100), 308 (31), 307 (M+ + 1, 23), 305 (17), 77 (14).
1-(4-Bromophenyl)-3-phenyl-1H-pyrazolo[3,4-d]pyrimidine (3i) [13,14,15]. Yellow solid; m.p. 180–181 °C (hexane–EtOAc). 1H-NMR (CDCl3, 400 MHz): δ 7.51–7.59 (3H, m, ArH), 7.66 (2H, d, J = 8.40 Hz, ArH), 8.05 (2H, d, J = 8.00 Hz, ArH), 8.29 (2H, d, J = 8.00 Hz, ArH), 9.13 (1H, s), 9.51 (1H, s). 13C-NMR (CDCl3, 100 MHz): δ 114.37, 119.99, 122.53 (2 × C), 127.37 (2 × C), 129.23 (2 × C), 129.79, 131.22, 132.24 (2 × C), 137.63, 145.29, 152.88, 153.40, 155.67. IR (KBr): 1586, 1555. 1481, 1400, 1389, 1215, 1072 cm–1. EIMS m/z: 350 (M+, 100), 352 (M+ + 2, 99), 353 (15), 351 (27), 194 (14), 77 (30).
3-Methyl-1-phenyl-1H-pyrazolo[3,4-d]pyrimidine (3j) [13,14,15,46]. Brown solid; m.p. 77–78 °C (hexane–EtOAc). 1H-NMR (CDCl3, 400 MHz): δ 2.70 (3H, s, CH3), 7.32 (1H, dd, J = 7.20, 14.80 Hz, ArH), 7.50–7.79 (2H, dd, J = 7.60, 15.60 Hz, ArH), 8.19 (2H, d, J = 8.00 Hz, ArH), 9.07 (1H, s), 9.16 (1H, s). 13C-NMR (CDCl3, 100 MHz): δ 12.59, 115.79, 121.07 (2 × C), 126.49, 129.22 (2 × C), 138.50, 143.35, 151.77, 152.77, 155.70. IR (KBr): 3240, 1643, 1503, 1439, 1211 cm–1. EIMS m/z: 210 (M+, 100), 211 (16), 209 (27), 195 (13), 142 (15), 77 (37), 69 (11), 57 (16), 55 (13), 51 (13).
3-tert-Butyl-1-phenyl-1H-pyrazolo[3,4-d]pyrimidine (3k) [13,14,15]. Yellow solid; m.p. 45–46 °C (hexane–EtOAc). 1H-NMR (CDCl3, 400 MHz): δ 1.57 (9H, s, t-Bu), 7.28–7.32 (1H, m, ArH), 7.51 (2H, dd, J = 7.60, 15.60 Hz, ArH), 8.22 (2H, d, J = 8.00 Hz, ArH), 9.04 (1H, s), 9.32 (1H, s). 13C-NMR (CDCl3, 100 MHz): δ 30.05 (3 × C), 34.51, 114.02, 121.17 (2 × C), 126.33, 129.13 (2 × C), 138.66, 152.84, 153.16, 154.88, 155.04. IR (KBr): 3048, 2967, 2666, 1636, 1578, 1508, 1427, 1366, 1188, 1096 cm–1. EIMS m/z: 252 (M+, 43), 238 (18), 237 (100), 222 (12), 105(11), 77(17), 57(11).
3-(4-Methylphenyl)-1-phenyl-1H-pyrazolo[3,4-d]pyrimidine (3l) [13,14,15]. White solid; m.p. 138–139 °C (hexane–EtOAc). 1H-NMR (CDCl3, 400 MHz): δ 2.44 (3H, s, CH3), 7.36 (3H, d, J = 6.80 Hz, ArH), 7.55 (2H, dd, J = 8.00, 16.00 Hz, ArH), 7.95 (2H, d, J = 8.00 Hz, ArH), 8.30 (2H, d, J = 8.00 Hz, ArH), 9.12 (1H, s), 9.49 (1H, s). 13C-NMR (CDCl3, 100 MHz): δ 21.39, 114.24, 121.41 (2 × C), 126.74, 127.23 (2 × C), 128.64, 129.18 (2 × C), 129.87 (2 × C), 138.51, 139.77, 145.06, 152.79, 153.27, 155.52. IR (KBr): 3117, 1582, 1501, 1223, 1092 cm–1. EIMS m/z: 286 (M+, 100), 287 (21), 285 (26).
3-(4-Chlorophenyl)-1-phenyl-1H-pyrazolo[3,4-d]pyrimidine (3m) [13,14,15,45]. Light-yellow solid; m.p. 194–193 °C (hexane–EtOAc). 1H-NMR (CDCl3, 400 MHz): δ 7.37 (1H, dd, J = 7.60, 15.20 Hz, ArH), 7.51–7.57 (m, 4 H, ArH), 8.00 (2H, d, J = 8.00 Hz, ArH), 8.28 (2H, d, J = 8.00 Hz, ArH), 9.13 (1H, s), 9.47 (1H, s). 13C-NMR (CDCl3, 100 MHz): δ 114.03, 121.47 (2 × C), 127.01, 128.51 (2 × C), 129.27 (2 × C), 129.48 (2 × C), 129.98, 135.71, 138.43, 143.85, 152.60, 153.37, 155.69. IR (KBr): 1632, 1555, 1504, 1404, 1219, 1092 cm–1. EIMS m/z: 306 (M+, 100), 308 (33), 307 (M+ + 1, 26), 305 (22), 77 (10).
3-(4-Methoxylphenyl)-1-phenyl-1H-pyrazolo[3,4-d]pyrimidine (3n) [13,14,15]. Light-yellow solid; m.p. 169–170 °C (hexane–EtOAc). 1H-NMR (CDCl3, 400 MHz): δ 3.86 (3H, s, OCH3), 7.04 (2H, d, J = Hz, ArH), 7.33 (1H, dd, J = 7.60, 14.80 Hz, ArH), 7.52 (2H, dd, J = 7.60, 15.60 Hz, ArH), 7.96 (2H, d, J = 8.00 Hz, ArH), 8.28 (2H, d, J = 8.00 Hz, ArH), 9.08 (1H, s), 9.43 (1H, s). 13C-NMR (CDCl3, 100 MHz): δ 55.36 (OCH3), 114.13, 114.57 (2 × C), 121.26 (2 × C), 124.01, 126.61, 128.61 (2 × C), 129.14 (2 × C), 138.51, 144.74, 152.66, 153.18, 155.43, 160.72. IR (KBr): 3059, 1632, 1613, 1528, 1501, 1431, 1362, 1300, 1258, 1219, 1173, 1092 cm–1. EIMS m/z: 302 (M+, 100), 303 (22), 287 (23), 77 (15).

4. Conclusions

We have successfully developed the one-flask method to synthesize pyrazolo[3,4-d]pyrimidines by treating 5-amino-pyrazoles, in presence of PBr3 coupling agent and then hexamethyldisilazane. In this new one-flask reaction was contained Vilsmeier reaction and the sequential intermolecular heterocyclization two steps. Based on the improved studies of the different type of Vilsmeier agents and amines, we found the commercial available DMF/PBr3 and hexamethyldisilazane were the best Vilsmeier agent and the efficient base for this newly developed one-flask synthesis. For the mechanistic study, 4-(iminomethyl)-1,3-diphenyl-1H-pyrazol-5-yl-N,N-disubstituted formamidines were demonstrated as the reaction intermediates by using a series of 4-formyl-1,3-diphenyl-1H-pyrazol-5-yl-N,N-disubstituted formamidines successfully reacted with amines to give pyrazolo[3,4-d]pyrimidines due to they were conceived as the chemical equivalent species. On the other hands, the order of reactivity of amines in intermolecular heterocyclization was NH(SiMe3)2 > NaN(SiMe3)2 > LiN(SiMe3)2 > hexamethylenetetramine. Through the further comparison variation reactive study between intramolecular and intermolecular Vilsmeier heterocyclization reaction, we found the intramolecular heterocyclization be able to provide the better results.

Supplementary Materials

Supplementary materials are available online.

Acknowledgments

We are grateful to the Tsuzuki Institute for Traditional Medicine and the Ministry of Science and Technology of the Republic of China (MOST 105-2113-M-039-001) for financial support.

Author Contributions

Fung Fuh Wong conceived and designed the experiments; Wan-Ping Yen and Shuo-En Tsai performed the experiments; Naoto Uramaru, Hiroyuki Takayama, Shuo-En Tsai, and Wan-Ping Yen analyzed the data; Fung Fuh Wong, Hiroyuki Takayama, and Naoto Uramaru contributed reagents/materials/analysis tools; Fung Fuh Wong, Wan-Ping Yen, and Shuo-En Tsai wrote the paper.

Conflicts of Interest

The authors declare no conflict of interest.

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Sample Availability: Samples of the compounds are available from the authors.
Molecules 22 00820 sch001 550
Scheme 1. Synthesis of pyrazolo[3,4-d]pyrimidines by the different synthetic strategy via the Vilsmeier reaction and intramolecular or intermolecular heterocyclization.
Scheme 1. Synthesis of pyrazolo[3,4-d]pyrimidines by the different synthetic strategy via the Vilsmeier reaction and intramolecular or intermolecular heterocyclization.
Molecules 22 00820 sch001
Molecules 22 00820 sch002 550
Scheme 2. The newly developed one-flask for synthesis of pyrazolo[3,4-d]pyrimidine derivatives via Vilsmeier reaction and the sequential heterocyclization.
Scheme 2. The newly developed one-flask for synthesis of pyrazolo[3,4-d]pyrimidine derivatives via Vilsmeier reaction and the sequential heterocyclization.
Molecules 22 00820 sch002
Molecules 22 00820 sch003 550
Scheme 3. A plausible mechanism for the newly developed one-flask procedure for the synthesis of pyrazolo[3,4-d]pyrimidines.
Scheme 3. A plausible mechanism for the newly developed one-flask procedure for the synthesis of pyrazolo[3,4-d]pyrimidines.
Molecules 22 00820 sch003
Table
Table 1. The study of amine agents in the one-flask for synthesis of pyrazolo[3,4-d]pyrimidines.
Table 1. The study of amine agents in the one-flask for synthesis of pyrazolo[3,4-d]pyrimidines.
EntryAmine AgentsEquiv.Yields (%) of Compound 3a
1Without base-- a
2Hexamethylenetetramine333
3Lithium bis(trimethylsilyl)amine (LiN(SiMe3)2)367
4Sodium bis(trimethylsilyl)amine (NaN(SiMe3)2)381
5Hexamethyldisilazane (NH(SiMe3)2)391
6Hexamethyldisilazane (NH(SiMe3)2)156
7Hexamethyldisilazane (NH(SiMe3)2)263
8Hexamethyldisilazane (NH(SiMe3)2)475
a 1H-pyrazol-5-yl-N,N-disubstituted formamidine 2a was isolated.
Table
Table 2. The study of the reactivity of the different Vilsmeier agents in the one-flask synthesis of pyrazole[3,4-d]pyrimidines.
Table 2. The study of the reactivity of the different Vilsmeier agents in the one-flask synthesis of pyrazole[3,4-d]pyrimidines.
Molecules 22 00820 i001
EntryAmide SolventsYields (%) of Compound 3a
1N,N-dimethylformamide (DMF)91
2N,N-diethylformamide (DEF)86
3N,N-diisopropylformamide83
4N,N-di-n-butylformamide81
5piperidine-1-carbaldehyde69
6pyrrolidine-1-carbaldehyde56
Table
Table 3. The results of the one-flask synthesis of pyrazolo[3,4-d]pyrimidines from 5-aminopyrazoles, DMF/PBr3 and NH(SiMe3)2.
Table 3. The results of the one-flask synthesis of pyrazolo[3,4-d]pyrimidines from 5-aminopyrazoles, DMF/PBr3 and NH(SiMe3)2.
Molecules 22 00820 i002
SubstratesXWNo.Yields of 3a–n (%)
Intermolecular ReactionIntramolecular Reaction a
1aPhPh3a9196
1bo-Me-PhPh3b7893
1co-Cl-PhPh3c8691
1dm-Me-PhPh3d8992
1em-Cl-PhPh3e9192
1fm-NO2-PhPh3f8787
1gp-Me-PhPh3g91-
1hp-Cl-PhPh3h8791
1ip-Br-PhPh3i8195
1jPhMe3j7993
1kPht-Bu3k8791
1lPhp-Me-Ph3l8493
1mPhp-Cl-Ph3m8891
1nPhp-OMe-Ph3n9194
a the reported data has been published and cited in ref. [13,14,15].
Table
Table 4. The mechanistic study for the intermolecular heterocyclization from 4-formyl-1,3-disubstituted-1H-pyrazol-5-yl-formamidines 7a–n with various amines.
Table 4. The mechanistic study for the intermolecular heterocyclization from 4-formyl-1,3-disubstituted-1H-pyrazol-5-yl-formamidines 7a–n with various amines.
Molecules 22 00820 i003
EntrySubstratesNR1R2AminesYields of 3a (%)
17aNMe2Hexamethylenetetramine3a37
27aNMe2LiN(SiMe3)23a51
37aNMe2NaN(SiMe3)23a84
47aNMe2NH(SiMe3)23a91
57bNEt2NH(SiMe3)23a89
67cN(i-Pr)2NH(SiMe3)23a61
77dN(n-Bu)2NH(SiMe3)23a81
87ePiperidinylNH(SiMe3)23a86

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MDPI and ACS Style

Yen, W.-P.; Tsai, S.-E.; Uramaru, N.; Takayama, H.; Wong, F.F. One-Flask Synthesis of Pyrazolo[3,4-d]pyrimidines from 5-Aminopyrazoles and Mechanistic Study. Molecules 2017, 22, 820. https://doi.org/10.3390/molecules22050820

AMA Style

Yen W-P, Tsai S-E, Uramaru N, Takayama H, Wong FF. One-Flask Synthesis of Pyrazolo[3,4-d]pyrimidines from 5-Aminopyrazoles and Mechanistic Study. Molecules. 2017; 22(5):820. https://doi.org/10.3390/molecules22050820

Chicago/Turabian Style

Yen, Wan-Ping, Shuo-En Tsai, Naoto Uramaru, Hiroyuki Takayama, and Fung Fuh Wong. 2017. "One-Flask Synthesis of Pyrazolo[3,4-d]pyrimidines from 5-Aminopyrazoles and Mechanistic Study" Molecules 22, no. 5: 820. https://doi.org/10.3390/molecules22050820

APA Style

Yen, W. -P., Tsai, S. -E., Uramaru, N., Takayama, H., & Wong, F. F. (2017). One-Flask Synthesis of Pyrazolo[3,4-d]pyrimidines from 5-Aminopyrazoles and Mechanistic Study. Molecules, 22(5), 820. https://doi.org/10.3390/molecules22050820

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