Next Article in Journal
Methyl Salicylate Glycosides in Some Italian Varietal Wines
Next Article in Special Issue
Drug Repurposing as an Antitumor Agent: Disulfiram-Mediated Carbonic Anhydrase 12 and Anion Exchanger 2 Modulation to Inhibit Cancer Cell Migration
Previous Article in Journal
Bioactive Compounds and Stability of a Typical Italian Bakery Products “Taralli” Enriched with Fermented Olive Paste
Previous Article in Special Issue
Synthesis, Evaluation, and Mechanism Study of New Tepotinib Derivatives as Antiproliferative Agents
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Molecules
Volume 24
Issue 18
10.3390/molecules24183259
molecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Communication

Design and Synthesis of Benzimidazole-Chalcone Derivatives as Potential Anticancer Agents

by
Cheng-Ying Hsieh
1,
Pi-Wen Ko
2,
Yu-Jui Chang
1,
Mohit Kapoor
3,
Yu-Chuan Liang
4,
Hsueh-Liang Chu
5,
Hui-Hsien Lin
6,
Jia-Cherng Horng
1,* and
Ming-Hua Hsu
7,*
1
Department of Chemistry, National Tsing Hua University, Hsinchu 30013, Taiwan
2
Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu 30013, Taiwan
3
Chitkara University Institute of Engineering and Technology, Chitkara University, Punjab 140 401, India
4
Agricultural Biotechnology Research Center, Academia Sinica, Taipei 11529, Taiwan
5
Graduate Institute of Translational Medicine, College of Medicine and Technology, Taipei Medical University, Taipei 11031, Taiwan
6
Division of Radiotherapy, Department of Oncology, Taipei Veterans General Hospital, Taipei 11217, Taiwan
7
Department of Chemistry, National Changhua University of Education, Changhua 50007, Taiwan
*
Authors to whom correspondence should be addressed.
Molecules 2019, 24(18), 3259; https://doi.org/10.3390/molecules24183259
Submission received: 20 August 2019 / Revised: 4 September 2019 / Accepted: 5 September 2019 / Published: 6 September 2019
(This article belongs to the Collection Efficient Pharmaceutical and Chemical Approaches for Anticancer Therapy: Design, Preliminary Evaluations, and Further Developments)
Browse Figures
Review Reports Versions Notes

Abstract

:
Numerous reports have shown that conjugated benzimidazole derivatives possess various kinds of biological activities, including anticancer properties. In this report, we designed and synthesized 24 new molecules comprising a benzimidazole ring, arene, and alkyl chain-bearing cyclic moieties. The results showed that the N-substituted benzimidazole derivatives bearing an alkyl chain and a nitrogen-containing 5- or 6-membered ring enhanced the cytotoxic effects on human breast adenocarcinoma (MCF-7) and human ovarian carcinoma (OVCAR-3) cell lines. Among the 24 synthesized compounds, (2E)-1-(1-(3-morpholinopropyl)-1H-benzimidazol-2 -yl)-3-phenyl-2-propen-1-one) (23a) reduced the proliferation of MCF-7 and OVCAR-3 cell lines demonstrating superior outcomes to those of cisplatin.

Graphical Abstract

1. Introduction

Cancer is the uncontrolled growth of abnormal cells leading to profound changes in physiological function. Cancer cells, which can evade the immune system, influence the normal cells, molecules, and blood vessels that surround and feed a tumor. The tumors, which can grow and metastasize to other locations in the body, can potentially interfere with the digestive, nervous, and circulatory systems and release hormones that alter body functions. For anticancer drug development, designing molecules that can selectively inhibit the proliferation of abnormal cells with minimal or no effect on normal cells is critical. Therefore, developing anticancer drugs is of utmost importance worldwide [1,2,3].
Benzimidazole ring systems possess various applications in novel drug development. Benzimidazole is a naturally occurring bicyclic compound [4] consisting of a fused benzene and imidazole ring and is an integral part of vitamin B12. Because of the structural similarities of benzimidazoles with purine, they can easily interact with the biomolecules of living systems. Therefore, they have considerable potential for use in medicinal chemistry and are a critical pharmacophore in drug discovery [5,6,7].
Benzimidazole and its derivatives possess various biological activities, including antibacterial, [8] anti-tubercular, [9,10] antifungal, [11] antimicrobial, [9,11] antiprotozoal, [12,13] anti-HIV, [14] and antiviral activities, [15] and show potential as protein kinase inhibitors. [16] Achar et al. synthesized several 2-methylaminobenzimidazole derivatives (Figure 1, 1), that displayed potent in-vivo analgesic and anti-inflammatory activities. [17] On the basis of this information, Refaat et al. designed benzylidene cyanomethylbenzimidazole (Figure 1, 2), which showed excellent anticancer activity potential against human liver carcinoma (HEP-G2) cell lines. [18] Azam et al. reported that phenylbenzimidazole analogues (Figure 1, 3) possess potent anti-cancer activity against five human cancer cell lines. [19] Various potent drugs containing a benzimidazole nucleus are currently available on the market. For example, albendazole (for treatment of certain infections caused by worms such as pork tapeworm and dog tapeworm, Figure 1, 4) [13], omeprazole (a member of the group of drugs called proton pump inhibitors that decrease the amount of acid produced in the stomach, Figure 1, 5), and pimobendan (a calcium sensitizer and selective inhibitor of phosphodiesterase III with positive inotropic and vasodilator effects, Figure 1, 6). Albendazole also inhibits hepatocellular carcinoma cell proliferation under both in vitro and in vivo experimental conditions. [20] Therefore, the optimization of benzimidazole derivatives on the basis of their structures has attracted considerable attention in recent years.
Chalcones (Figure 2, 7) which belong to the flavonoid family, are open-chained molecules bearing an α,β-unsaturated carbonyl system between two aromatic rings and represent a crucial class of molecules that are abundant in edible plants. [21] Chalcones reportedly possess beneficial biological activities [22], particularly considering their similar mode of action to the structurally related natural phenol combretastatin (Figure 2, 8). [23] Many studies on chalcone derivatives have shown that they demonstrate antibacterial [24], antimalarial [25,26], antifungal [27], anti-HIV [28], anti-inflammatory [29], and anticancer activities [30,31,32,33]. Modzelewska et al. synthesized a series of bis-chalcones (Figure, 9) that demonstrated exceptional performance in inhibiting of the growth of human breast and colon cancer cells. [32]
On the basis of a literature survey, we synthesized benzimidazole-chalcone conjugates, which could lead to the development of potent anti-cancer agents. In the development of new drugs, combining various moieties with different biological activities may lead to the creation of novel candidates with exceptional pharmacological activity [34,35,36,37]. Some recent studies have proposed several hybrid benzimidazolyl-chalcone derivatives that display anthelmintic [38], antifungal [39] and antitumor activities [40]. Woo et al. showed that substituted benzimidazolyl curcumin mimics (Figure 3, 10) possess anticancer activity, and they hypothesized that the increment in inhibitory potency is due to the attached benzimidazole functionalities [41]. In addition, some benzimidazole derivatives serve as antagonists of the chemokine receptor CXCR3 [42] (Figure 3, 11), inhibitors of the hepatitis B virus [15] (Figure 3, 12), and inhibitors of Francisella tularensis enoyl-ACP reductase [43] (Figure 3, 13), and antitumor activities [44] (Figure 3, 14) by modifying some monomers on benzimidazole. Herein, we report the scope of activity of benzimidazole-chalcone conjugates as anti-cancer agents. Through an analysis of their anti-cancer activities on various cell lines influenced by the substituents on phenyl and nitrogen, their structure-activity relationship (SAR) guidelines were deduced.

2. Results and Discussion

2.1. Chemistry

The synthetic strategies adopted to obtain the target compounds are depicted in Scheme 1. Syntheses of benzimidazole-chalcone derivatives were achieved through conjugation of benzimidazole and aromatic aldehydes under basic conditions. Benzimidazole derivative 16 was synthesized in high yield [45] through a reaction of o-phenylenediamine with lactic acid (1.1 equiv) in hydrochloric acid (4.0 N concentration) under reflux for 6.0 h, which underwent oxidation in the presence of potassium permanganate (2.5 equiv) and solid aluminium oxide to afford the intermediate compound 17, which was prepared from the oxidation products. After being ground with a pestle and mortar and then purified, compound 17 was obtained in 72% yield as a white solid [46]. We prepared the benzimidazolyl-chalcones 18a–18d in 82–95% overall yields by reacting compound 17 with substituted aromatic aldehydes possessing 4-methyl- (18b), 4-methoxy- (18c), and 4-chloro- (18d) groups in the presence of a base, through conventional Claisen-Schmidt condensations. [35] To expand the structural diversity of the benzimidazolyl-chalcone derivatives, we synthesized substituted benzimidazolyl-chalcones 19–23 from allyl bromide or various alkyl chlorides bearing a hydrocarbon spacer and a nitrogen-containing 5- or 6-membered ring (1.2 equiv) in the presence of sodium carbonate and acetonitrile. For the preparation of the final compounds, compounds 18a–18d were reacted with allyl bromide, 4-(2-chloroethyl)morpholine, 1-(2-chloroethyl)-piperidine, 1-(2-chloroethyl)pyrrolidine or 4-(3-chloropropyl)morpholine (1.2 equiv) in acetonitrile with potassium carbonate (2.9 equiv) at reflux overnight to afford (2E)-1-(1-allyl-1H-benzimidazol-2-yl)-3-phenyl-2-propen-1-ones 19a–23a, (2E)-1-(1-allyl-1H-benzimidazol-2-yl)-3-(4-methylphenyl)-2-propen-1-ones 19b–23b, (2E)-1-(1-allyl-1H-benzimidazol-2-yl)-3-(4-methoxyphenyl)-2 -propen-1-ones 19c–23c, and (2E)-1-(1-allyl-1H-benzimidazol-2-yl)-3-(4-chlorophenyl)-2-propen-1-ones 19d–23d, respectively, in 50–86% overall yields. The characterization data of compounds are showed in the supporting information.

2.2. Pharmacology

To determine the SAR, we designed various types of benzimidazole-chalcone derivatives 18–23 having a phenyl ring substituted with electron-donating and electron-withdrawing groups as well as modified benzimidazolyl groups (allyl, nitrogen-containing 5- or 6-membered rings). All the new compounds were evaluated against four common types of human cancer, namely human lung carcinoma (A549), human breast adenocarcinoma (MCF-7), human hepatoma (HEP-G2), and human ovarian carcinoma (OVCAR-3) cell lines. For comparison purposes, the cytotoxicity of doxorubicin (a standard antitumor drug) and cisplatin (a platinum-containing anti-cancer drug) were evaluated under identical conditions. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assays were conducted. The MTT assay is used calculate the relationship between cell viability under different treatments [47,48,49]. The IC50 value (the dose of the compound that causes a 50% reduction in the survival value) found via MTT assay is used to evaluate the potential anticancer activity of compounds [50,51]. The IC50 values of compounds 18a–23d are summarized in Table 1.
Most of the compounds demonstrated potential antitumor activities against all of the tested tumor cell lines. Among the 24 benzimidazole derivatives, eight compounds showed IC50 values in the 9.73–12.47 μM range and six compounds in the 14.59–19.53 μM range on the A549 cell line. The IC50 of these 24 compounds on the MCF-7 cell line demonstrated promising activities; 10 of the compounds showed an IC50 between 8.91 and 12.12 μM. Regarding the HEP-G2 cell line, three compounds showed an IC50 in the 10.16–10.93 μM range. Finally, the IC50 values of these 24 compounds for the OVCAR-3 cell line were as follows: 11 compounds showed an IC50 in the range of 10.34–14.88 μM, 10 in the range of 16.09–36.48 μM, and the remaining compounds were higher than 42.24 μM. The differences in the IC50 values may be attributable to such factors as the nature of the N-substitution at the benzimidazole ring system and the functionality of the phenyl moiety on the 4 position of the phenyl ring system, and the genetic and biochemical background of the cell lines.
Generally, most of the tested compounds tended to be more active against MCF-7 and OVCAR-3 than against the other tumor cell lines. Six compounds (20a–23a, 22b, 22c) showed IC50 values of less than 11.70 μM (the IC50 value of cisplatin) on the MCF-7 cell line. In contrast to the OVCAR-3 cell line, 11 compounds (20a, 21a, 23a, 21b, 23b, 20c–23c, 20d, 22d) had IC50 values between 10.34 and 14.88 μM, which were less than 16.04 μM (the IC50 value of cisplatin). The most active compound among the tested benzimidazole derivatives was 23a, which exhibited IC50 values of 9.73, 8.91, 10.93 and 10.76 μM on the A549, MCF-7, HEP-G2 and OVCAR-3 cells, respectively. This compound showed in vitro cytotoxicity comparable or superior to that of cisplatin.
SAR studies have suggested that the introduction of a nitrogen-containing 5- or 6-membered ring bearing a hydrocarbon spacer group (20a, 21a, 23a) in the N-substitution pattern of (2E)-1-(1H-benzimidazol-2-yl)-3-phenyl-2-propen-1-one (18a) leads to an increase in cytotoxic activity on A549, MCF-7, and OVCAR-3 cells, compared with the unsubstituted compound 18a. Compared with compounds 23a–23d, (2E)-1-(1-(3-morpholinopropyl)-1H-benzimidazol-2-yl)-3-phenyl-2-propen-1-one (23a) was generally more active than the 4-methyl-, 4-methoxy- and 4-chloro- functionality on phenyl moieties (compounds 23b–23d) in A549, MCF-7 and OVCAR-3 cells. Similarly, among compounds 21a–21d and 20a–20d, we observed the same trend as in compounds 23a–23d. This result suggests that the hybridization of the chalcone and benzimidazole ring system, which is modified with a biological side chain bearing a hydrocarbon spacer and a nitrogen-containing 5- or 6-membered ring in the N-substitution, can lead to enhanced cytotoxic effects on MCF-7 and OVCAR-3 cells. Notably, these data show that some of the tested compounds are more potent than cisplatin.
In pharmacotherapy, most drugs are taken via oral administration and are absorbed through the gastrointestinal (GI) system [52]. Some drugs have to cross a series of barriers either by passive diffusion or carrier conjugation [53]. Therefore, lipophilicity is a very important index in anticancer drug development [52,53,54]. The ALOGPS software package predicts lipophilicity and aqueous solubility of chemical compounds [53,54,55]. In this software, logP, is accepted as the principal parameter through which to evaluate lipophilicity of chemical compounds which largely determines the pharmacokinetic properties of drugs [53].
In this study, no significant correlation was observed between the IC50 values and log P values of the 24 compounds (Table 1). Therefore, the difference in lipophilicity might not be a significant factor in the differences in cytotoxicity of the tested compounds. By contrast, we observed no correlation between the IC50 and water solubility values of these compounds, which differs from the modified benzimidazole ring system. However, in these compounds, which differ in the 4-methyl-, 4-methoxy-, and 4-chloro- functionality of their phenyl moiety, we observed a correlation between the IC50 and water solubility values (S (cal), Table 1). For example, the IC50 values of 23a (8.03 mmol/L water solubility) and 23b–23d (3.26–6.00 mmol/L water solubility) were 8.91 versus 11.34–35.69 μM (MCF-7) and 10.76 versus 13.76–42.24 μM (OVCAR-3), respectively (Table 1). Similarly, among compounds 18a–18d, 20a–20d and 20c, 21a, 21b, 21d, we observed the same trend as compared with compounds 23a–23d. Therefore, the difference in water solubility may be a factor explaining the difference in cytotoxicity of the benzimidazole derivatives examined in our study.
We conducted a cell cycle analysis through flow cytometry of compounds 20a–23a to elucidate their mechanism of action. OVCAR-3 cells were treated with compounds 20a–23a for 48 h. The histograms obtained from the OVCAR-3 cells demonstrated a major peak (G1) and minor peak (G2) (Figure S1). OVCAR-3 cells treated with 10.50 μM compound 20a and 10.34 μM compound 21a showed 55% and 63% in the G2/M phase, respectively, whereas an untreated control showed 37% in the G2/M phase with same value of 8% in the S phase (Table 2). By contrast, treatment of OVCAR-3 cells with the 22.44 μM compound 22a and 10.76 μM compound 23a did not alter the cell cycle distribution compared with the untreated control. Because compounds 20a and 21a could effectively induce cell cycle arrest and inhibit cancer cell growth at a similar concentration, our data suggest that the growth inhibition activities of compounds 20a and 21a are directly related to their abilities to arrest cell cycle progression. In contrast to 20a and 21a, compound 23a did not induce cell cycle arrest at 10 μM on OVCAR-3, whereas the IC50 values of compound 23a for cancer cells were observed at 10.76 μM (Table 1), therefore, the cytotoxic effects of 23a might not be due to cell cycle arrest.

3. Materials and Methods

3.1. General Procedures

All reactions were carried out in oven-dried glassware (120 °C) under an atmosphere of nitrogen. Acetone, acetonitrile, ethanol, dichloromethane, ethyl acetate and hexane from Mallinckrodt Chemical Co. (Staines-Upon-Thames, United Kingdom) were dried and distilled from CaH2. Allyl bromide, benzaldehyde, 4-chlorobenzaldehyde, 4-(2-chloroethyl)morpholine, 4-(3-chloropropyl)morpholine, 1-(2-chloroethyl)-piperidine, 1-(2-chloroethyl)pyrrolidine, lactic acid, 4-methylbenzaldehyde, 4-methoxybenzaldehyde,o-phenylenediamine, potassium carbonate, and potassium permanganate were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Hydrochloric acid, magnesium sulfate, potassium hydroxide, and sodium hydroxide were purchased from Showa (Tokyo, Japan). Aluminum oxide was purchased from Merck (Darmstadt, Germany).
Analytical thin layer chromatography (TLC) was performed on precoated plates (silica gel 60 F-254), purchased from Merck. Purification by gravity column chromatography was carried out by use of Merck Silica Gel 60 (particle size 0.063–0.200 mm, 70–230 mesh ASTM). 1H-NMR spectra were obtained on an Avance 500 (500 MHz) spectrometer (Bruker, Billerica, MA, USA) by use of chloroform-d all examples given use acetone and acetone-d6 as solvents. 1H-NMR chemical shifts were referenced to the CDCl3 singlet (7.24 ppm) and the acetone-d6 quintet (2.05 ppm). 13C-NMR spectra were obtained on Bruker Avance 500 (125 MHz), AM-400* (Bruker) and MR-400 (Varian, Palo Alto, CA, USA) all examples given use a 500 MHz instrument spectrometers by use of acetone-d6 as solvent. 13C-NMR chemical shifts were referenced to the center of the acetone-d6 septet (29.92 ppm). Multiplicities were recorded by the following abbreviations: brs, broad singlet; s, singlet; d, doublet; t, triplet; q, quartet; m, multiplet; J, coupling constant (hertz). High-resolution mass spectra were obtained by means of a FINNIGAN/MAT-95XL mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA). High-performance liquid chromatography (HPLC) analyses were carried out on an 1100 series system (Agilent, Santa Clara, CA, USA) equipped with a CNW Athena C18 column (120 Å, 4.6 mm × 250 mm, 5 μm) and UV detection at 254 nm. A mixture of 20% DI water in acetonitrile was used as eluent and flow rate was at 0.5 mL/min. Infrared (IR) spectra were measured on a RX 1FT-IR spectrometer (Perkin Elmer, Waltham, MA, USA). Absorption intensities were recorded using the following abbreviations: s, strong; m, medium; w, weak; br, broad.

3.2. Synthesis

3.2.1. Synthesis of 1-(1H-benzoimidazol-2-yl)ethan-1-ol (16).

Hydrochloric acid (4.0 N, 25 mL) was added to a stirred solution of o-phenylenediamine (4.32 g, 40.0 mmol, 1.0 equiv) in lactic acid (3.96 g, 44.0 mmol, 1.1 equiv) and the reaction mixture was heated to reflux for 16 h. After 16 h, the reaction mixture was cooled down to room temperature and neutralized with sodium hydroxide solution. The reaction mass was filtered to obtain compound 16 (6.15 g, 38.0 mmol) in 95% yield as a pale yellow solid: 1H-NMR (CDCl3, 500 MHz) δ 1.73 (d, J = 6.0 Hz, 3 H, CH3), 5.22 (q, J = 6.0 Hz, 1 H, CH), 7.26 (d, J = 6.0 Hz, 1 H, ArCH), 7.27 (d, J = 5.5 Hz, 1 H, ArCH), 7.59 (d, J = 5.5 Hz, 1 H, ArCH), 7.60 (d, J = 6.0 Hz, 1 H, ArCH).

3.2.2. Synthesis of 1-(1H-benzimidazol-2-yl)ethan-1-one (17).

First, alumina-supported permanganate was prepared by mixing solid KMnO4 (2.0 g, 12.65 mmol, 2.5 equiv) and solid aluminium oxide (2.5 g) in a mortar ground with a pestle for 3.0 min. Then compound (16, 0.810 g, 4.99 mmol, 1.0 equiv) was added in the mortar and stirred for another 10 min. Acetone (40 mL) was added to the reaction mixture in a beaker and stirred for 20 min. The mixture was filtered and the filtrate was evaporated to obtain a crude residue. The organic mass was extracted with EtOAc (2 × 10 mL) and washed with H2O (2 × 5.0 mL), dried over MgSO4(s), filtered, and concentrated under reduced pressure. The residue was purified by use of column chromatography (10% ethyl acetate in hexane as eluent) to give the desired compound 17 (0.580 g, 3.62 mmol) in 72% yield as white solids. 1H-NMR (CDCl3, 400 MHz) δ 2.81 (s, 3 H, CH3), 7.35 (dd, J = 8.0 Hz, 1 H, ArCH), 7.41 (dd, J = 7.5 Hz, 1 H, ArCH), 7.53 (d, J = 8.0 Hz, 1 H, ArCH), 7.90 (d, J = 8.0 Hz, 1 H, ArCH).

3.2.3. Standard Procedure 1 for the Synthesis of Benzimidazolyl-Chalcone Derivatives 18a–18d.

Compound 18a–18d had been reported in previous literatures [56]. Aqueous KOH (40%) was added to a stirred solution of 2-acetylbenzimidazole (17, 1.0 equiv) and substituted aromatic aldehydes (1.1 equiv) in ethanol. The resulting mixture was stirred at room temperature for 10 h. After the consumption of starting materials, the reaction mixture was quenched with water (2–4 mL) and extracted with EtOAc (10–20 mL). The combined organic layers were washed with brine (5.0 mL), dried over MgSO4(s), filtered, and concentrated under reduced pressure. The residue was purified by column chromatography to give the desired benzimidazolyl-chalcone derivatives.

3.2.4. Standard Procedure 2 for Synthesis of Side Chain Modified Benzimidazolyl-chalcone Derivatives 19–23.

Potassium carbonate (3.0 equiv) was added to a stirred solution of benzimidazolyl-chalcone derivatives, 18a–18d (1.0 equiv) and substituted halides (1.2 equiv) in acetonitrile and the resulting solution was heated at reflux overnight. The reaction mixture was cooled to room temperature, quenched with water (2.0–4.0 mL), and extracted with EtOAc (10–20 mL). The combined organic layers were washed with brine (5.0 mL), dried over MgSO4(s), filtered, and concentrated under reduced pressure. The residue was purified by column chromatography to give the desired side chain modified benzimidazolyl-chalcone derivatives.

3.3. Compound Data

(2E)-1-(1H-benzimidazol-2-yl)-3-phenyl-2-propen-1-one (18a). Standard procedure 1 was followed by use of compound 17 (0.640 g, 4.0 mmol, 1.0 equiv), benzaldehyde (0.467 g, 4.4 mmol, 1.1 equiv), and aq. KOH (40%, 2.0 mL) in ethanol (8.0 mL). After workup and purification with column chromatography (15% EtOAc in hexane as eluent), compound 18a (0.870 g, 3.51 mmol) was obtained in 88% yield as yellow solids: 1H-NMR (acetone-d6, 500 MHz) δ 7.37 (t, J = 7.5 Hz, 1 H, ArCH), 7.44 (t, J = 7.5 Hz, 1 H, ArCH), 7.52-7.53 (m, 3 H, 3 × ArCH), 7.68 (d, J = 8.0 Hz, 1 H, ArCH), 7.88 (d, J = 7.5 Hz, 2 H, 2 × ArCH), 7.88 (d, J = 7.5 Hz, 1 H, ArCH), 8.03 (d, J = 16 Hz, 1 H, COCH), 8.17 (d, J = 16.0 Hz, 1 H, PhCH); 13C-NMR (acetone-d6, 125 MHz) δ 113.64, 122.45, 122.55, 124.18, 126.82, 128.97, 129.78, 130.08, 131.77, 131.91, 135.81, 144.70, 145.23, 146.01, 150.20, 181.92; IR (neat) 3357 (s), 3247 (N-H, s), 2920 (s), 2850 (s), 1661 (C=O, s), 1632 (m), 1597 (C=N, s), 1424 (s), 1331 (C–N, s), 1215 (m), 1138 (w), 1089 (w), 971 (w), 741 (w), 721.59 (w); MS (ESI) m/z calcd for C16H12N2O: 248.0950, found: 248.0950. Its spectroscopic characteristics are consistent with those reported for the same compound [56].
(2E)-1-(1H-benzimidazol-2-yl)-3-(4-methylphenyl)-2-propen-1-one (18b). Standard procedure 1 was followed by use of compound 17 (0.640 g, 4.0 mmol, 1.0 equiv), 4-methylbenzaldehyde (0.529 g, 4.4 mmol, 1.1 equiv), and aq. KOH (40%, 2.0 mL) in ethanol (8.0 mL). After workup and purification with column chromatography (20% EtOAc in hexane as eluent), compound 18b (0.942 g, 3.59 mmol) was obtained in 90% yield as yellow solids: 1H-NMR (acetone-d6, 500 MHz) δ 2.43 (s, 3 H, CH3), 7.34 (d, J = 8.0 Hz, 2 H, 2 × ArCH), 7.39 (d, J = 6.0 Hz, 1 H, ArCH), 7.40 (d, J = 7.5 Hz, 1 H, ArCH), 7.76 (d, J = 7.5 Hz, 4 H, 4 × ArCH), 7.99 (d, J = 16 Hz, 1 H, COCH), 8.13 (d, J = 16 Hz, 1H, PhCH); 13C-NMR (acetone-d6, 125 MHz) δ 21.60, 113.63, 121.56, 122.42, 124.15, 126.75, 129.86, 130.78, 132.23, 133.14, 135.80, 142.52, 144.73, 145.33, 181.92; IR (neat) 3839 (s), 3736 (s), 3649 (m), 3568 (m), 3362 (w), 3247 (N–H, br), 2920 (s), 2846 (s), 2363 (s), 1657 (C=O, s), 1594 (C=N, m), 1424 (w), 1328 (C–N, m), 1218 (s), 1089 (m), 982 (m), 809 (w), 738 (m); MS (ESI) m/z calcd for C17H14N2O: 262.1106, found: 262.1107. Its spectroscopic characteristics are consistent with those reported for the same compound [56].
(2E)-1-(1H-benzimidazol-2-yl)-3-(4-methoxyphenyl)-2-propen-1-one (18c). Standard procedure 1 was followed by use of compound 17 (0.640 g, 4.0 mmol, 1.0 equiv), 4-methoxybenzaldehyde (0.598 g, 4.4 mmol, 1.1 equiv), and aq. KOH (40%, 2.0 mL) in ethanol (8.0 mL). After workup and purification with column chromatography (20% EtOAc in hexane as eluent), compound 18c (1.05 g, 3.78 mmol) was obtained in 95% yield as yellow solids: 1H-NMR (acetone-d6, 500 MHz) δ 3.90 (s, 3 H, OCH3), 7.08 (d, J = 9.0 Hz, 2 H, 2 × ArCH), 7.35 (t, J = 7.5 Hz, 1 H, ArCH), 7.42 (t, J = 7.5 Hz, 1 H, ArCH), 7.68 (d, J = 8.5 Hz, 1 H, ArCH), 7.84 (d, J = 9.0 Hz, 2 H, 2 × ArCH), 7.87 (d, J = 8.0 Hz, 1 H, ArCH), 7.99 (d, J = 16 Hz, 1 H, COCH), 8.04 (d, J = 16 Hz, 1 H, PhCH); 13C-NMR (acetone-d6, 125 MHz) δ 55.97, 113.59, 115.56, 120.09, 122.37, 124.08, 126.63, 128.45, 131.72, 135.76, 144.73, 145.21, 150.44, 163.29, 181.81; IR (neat) 3357 (s), 3192 (N–H, s), 2920 (s), 2850 (s), 2313 (w), 1756 (br), 1657 (C=O, s), 1634 (s), 1586 (C=N, m), 1514 (m), 1468 (m), 1421 (m), 1369 (w) 1322 (C–N, w) 1243 (s), 1172 (m), 1089 (w), 1056 (w), 823 (w), 718 (w), 631 (w); MS (ESI) m/z calcd for C17H14N2O2: 278.1055, found: 278.1055. Its spectroscopic characteristics are consistent with those reported for the same compound [56].
(2E)-1-(1H-benzimidazol-2-yl)-3-(4-chlorophenyl)-2-propen-1-one (18d). Standard procedure 1 was followed by use of compound 17 (0.640 g, 4.0 mmol, 1.0 equiv), 4-chlorobenzaldehyde (0.616 g, 4.4 mmol, 1.1 equiv), and aq. KOH (40%, 2.0 mL) in ethanol (8.0 mL). After workup and purification with column chromatography (20% EtOAc in hexane as eluent), compound 18d (0.976 g, 3.45 mmol) was obtained in 86% yield as yellow solids: 1H-NMR (acetone-d6, 500 MHz) δ 7.37 (t, J = 7.5 Hz, 1 H, ArCH), 7.44 (t, J = 7.5 Hz, 1 H, ArCH), 7.55 (d, J = 8.5 Hz, 2 H, 2 × ArCH), 7.68 (d, J = 8.5 Hz, 1 H, ArCH), 7.88 (d, J = 8.5 Hz, 1 H, ArCH), 7.89 (d, J = 8.5 Hz, 2 H, 2 × ArCH), 7.99 (d, J = 16 Hz, 1 H, COCH), 8.16 (d, J = 16 Hz, 1 H, PhCH); 13C-NMR (acetone-d6, 125 MHz) δ 113.55, 122.37, 123.18, 124.12, 126.79, 128.95, 130.10, 131.23, 133.33, 134.59, 135.72, 137.01, 143.54, 144.61, 149.99, 181.69; IR (neat) 3841 (s), 3736 (s), 3648 (m), 2918 (w), 2846 (w), 2357 (s), 1772 (w), 1646 (C=O, s), 1599 (C=N, s), 1501 (br), 1405 (m), 1327 (C–N, s) 1218 (s), 1084 (s), 977 (s), 823 (s) 765 (w), 744 (s) 642 (w), 546 (w); MS (ESI) m/z calcd for C16H11ClN2O: 282.0560, found: 282.0562. Its spectroscopic characteristics are consistent with those reported for the same compound [56].
(2E)-1-(1-Allyl-1H-benzimidazol-2-yl)-3-phenyl-2-propen-1-one (19a). Standard procedure 2 was followed by use of compound 18a (0.124 g, 0.50 mmol, 1.0 equiv) and allyl bromide (0.0726 g, 0.60 mmol, 1.2 equiv) in acetonitrile (15 mL) to which potassium carbonate (0.200 g, 1.45 mmol, 2.9 equiv) was added. After workup and purification with column chromatography (20% EtOAc in hexane as eluent), compound 19a (0.110 g, 0.38 mmol) was obtained in 76% yield as yellow solids: 1H-NMR (acetone-d6, 500 MHz) δ 5.07 (d, J = 17.0 Hz, 1 H, CHCH2(trans)), 5.16 (d, J = 10.0 Hz, 1 H, CHCH2(cis)), 5.44 (d, J = 5.0 Hz, 2 H, NCH2), 6.09–6.16 (m, 1H, CHCH2), 7.40 (t, J = 7.5 Hz, 1 H, ArCH), 7.47 (t, J = 7.5 Hz, 1 H, ArCH), 7.50-7.51 (m, 3 H, 3 × ArCH), 7.67 (d, J = 8.5 Hz, 1 H, ArCH), 7.84 (d, J = 7.5 Hz, 2 H, 2 × ArCH), 7.88 (d, J = 8.5 Hz, 1 H, ArCH), 7.90 (d, J = 16.0 Hz, 1H, CCOCH), 8.31 (d, J = 16.0 Hz, 1H, PhCH); 13C-NMR (acetone-d6, 125 MHz) δ 48.26, 112.44, 117.30, 122.55, 124.13, 124.62, 126.87, 129.72, 130.06, 131.80, 134.46, 135.82, 137.54, 142.84, 144.74, 147.66, 183.18; IR (neat) 3789 (s), 3696 (w), 3663 (w), 3574 (w), 3355 (m), 2920 (s), 2850 (s), 1666 (C=O, s), 1605 (C=N, s), 1473 (w), 1449 (s), 1407 (w), 1332 (C–N, s), 1303 (w), 1165 (m), 1051 (s), 988 (m), 743 (s), 560 (m); MS (ESI) m/z calcd for C19H16N2O: 288.1263, found: 288.1264.
(2E)-1-(1-Allyl-1H-benzimidazol-2-yl)-3-(4-methylphenyl)-2-propen-1-one (19b). Standard procedure 2 was followed by use of compound 18b (0.131 g, 0.50 mmol, 1.0 equiv) and allyl bromide (0.0726 g, 0.60 mmol, 1.2 equiv) in acetonitrile (15 mL) to which potassium carbonate (0.200 g, 1.45 mmol, 2.9 equiv) was added. After workup and purification with column chromatography (20% EtOAc in hexane as eluent), compound 19b (0.088 g, 0.29 mmol) was obtained in 58% yield as yellow solids: 1H-NMR (acetone-d6, 500 MHz) δ 2.47 (s, 3H, CH3), 5.06 (d, J = 17.0 Hz, 1 H, CHCH2(trans)), 5.16 (d, J = 10 Hz, 1 H, CHCH2(cis)), 5.43 (d, J = 5.0 Hz, 2 H, NCH2), 6.08-6.15 (m, 1 H, CHCH2), 7.32 (d, J = 8.0 Hz, 2 H, 2 × ArCH), 7.39 (t, J = 7.5 Hz, 1H, ArCH), 7.47 (t, J = 7.5 Hz, 1 H, ArCH), 7.66 (d, J = 7.5 Hz, 1 H, ArCH), 7.72 (d, J = 8.5 Hz, 2 H, 2 × ArCH), 7.87 (d, J = 16.0 Hz, 1 H, COCH), 7.88 (d, J = 8.0 Hz, 1H, ArCH), 8.26 (d, J = 16.0 Hz, 1H, PhCH); 13C-NMR (acetone-d6, 125 MHz) δ 21.59, 48.27, 112.40, 117.28, 122.58, 123.20, 124.53, 126.75, 129.78, 130.74, 133.17, 134.54, 135.58, 142.35, 142.94, 144.74, 147.80, 183.23; IR (neat) 3835 (s), 3731 (s), 2917 (m), 2846 (w), 2346 (s), 1748 (s), 1657 (C=O, br), 1597 (C=N, m), 1509 (w), 1375 (w), 1339 (C–N, w), 1231 (s), 1204 (w) 1180 (w), 1163 (m), 1048 (w), 985 (w), 812 (m), 752 (w), 738 (w), 724 (w); MS (ESI) m/z calcd for C20H18N2O: 302.1419, found: 302.1417.
(2E)-1-(1-Allyl-1H-benzimidazol-2-yl)-3-(4-methoxyphenyl)-2-propen-1-one (19c). The standard procedure 2 was followed by use of compound 18c (0.139 g, 0.50 mmol, 1.0 equiv) and allyl bromide (0.0726 g, 0.60 mmol, 1.2 equiv) in acetonitrile (15 mL) to which potassium carbonate (0.200 g, 1.45 mmol, 2.9 equiv) was added. After workup and purification with column chromatography (20% EtOAc in hexane as eluent), compound 19c (0.116 g, 0.36 mmol) was obtained in 72% yield as yellow solids: 1H-NMR (acetone-d6, 500 MHz) δ 3.89 (s, 3 H, OCH3), 5.07 (d, J = 17.0 Hz, 1 H, CHCH2(trans)), 5.16 (d, J = 10.0 Hz, 1 H, CHCH2(cis)), 5.43 (d, J = 5.0 Hz, 2 H, NCH2), 6.08–6.15 (m, 1 H, CHCH2), 7.06 (d, J = 10 Hz, 2 H, 2 × ArCH), 7.39 (t, J = 7.5 Hz, 1 H, ArCH), 7.46 (t, J = 7.5 Hz, 1 H, ArCH), 7.66 (d, J = 8.0 Hz, 1 H, ArCH), 7.80 (d, J = 8.5 Hz, 2 H, 2 × ArCH), 7.87 (d, J = 8.5 Hz, 1 H, ArCH), 7.87 (d, J = 16.0 Hz, 1 H, COCH), 8.17 (d, J = 16.0 Hz, 1H, PhCH); 13C-NMR (acetone-d6, 125 MHz) δ 48.25, 55.95, 112.38, 115.53, 117.26, 121.78, 122.51, 124.48, 126.64, 128.49, 131.64, 134.58, 137.55, 142.92, 144.68, 147.92, 163.19, 183.18; IR (neat) 3789 (s), 3357 (s), 3187 (w), 2920 (s), 2850 (s), 1659 (C=O, s), 1591 (C=N, s), 1330 (C–N, m), 1292 (m), 1254 (s), 1169 (s), 1054 (s), 988 (m), 826 (s), 741 (w); MS (ESI) m/z calcd for C20H18N2O2: 318.1368, found: 318.1367.
(2E)-1-(1-Allyl-1H-benzimidazol-2-yl)-3-(4-chlorophenyl)-2-propen-1-one (19d). Standard procedure 2 was followed by use of compound 18d (0.141 g, 0.50 mmol, 1.0 equiv) and allyl bromide (0.0726 g, 0.60 mmol, 1.2 equiv) in acetonitrile (15 mL) to which potassium carbonate (0.200 g, 1.45 mmol, 2.9 equiv) was added. After workup and purification with column chromatography (20% EtOAc in hexane as eluent), compound 19d (0.123 g, 0.38 mmol) was obtained in 76% yield as yellow solids: 1H-NMR (acetone-d6, 500 MHz) δ 5.06 (d, J = 17.0 Hz, 1 H, CHCH2(trans)), 5.16 (d, J = 10.0 Hz, 1 H, CHCH2(cis)), 5.41 (d, J = 5.0 Hz, 2 H, NCH2), 6.07–6.15 (m, 1 H, CHCH2), 7.39 (t, J = 7.5 Hz, 1 H, ArCH), 7.47 (t, J = 7.5 Hz, 1 H, ArCH), 7.52 (d, J = 8.0 Hz, 2H, 2 × ArCH), 7.65 (d, J = 8.0 Hz, 1H, ArCH), 7.85 (d, J = 7.5 Hz, 2H, 2 × ArCH), 7.86 (d, J = 8.5 Hz, 1H, ArCH), 7.87 (d, J = 16.0 Hz, 1H, COCH), 8.29 (d, J = 16.0 Hz, 1H, PhCH); 13C-NMR (acetone-d6, 125 MHz) δ 48.27, 112.42, 117.33, 122.64, 124.61, 124.87, 126.90, 130.16, 131.23, 134.46, 134.70, 136.97, 137.60, 142.93, 143.02, 147.58, 182.99; IR (neat) 3789 (s), 3352 (s), 3187 (m), 2920 (s), 2850 (s), 1663 (C=O, s), 1605 (C=N, s), 1477 (m), 1449 (w), 1402 (w), 1334 (C–N, s), 1300 (m), 1232 (m), 1163 (m), 1056 (s), 988 (s), 919 (m), 830 (s), 742 (s); MS (ESI) m/z calcd for C19H15ClN2O: 322.0873, found: 322.0873.
(2E)-3-Phenyl-1-(1-(2-(pyrrolidin-1-yl)ethyl)-1H-benzimidazol-2-yl)-2-propen-1-one (20a). Standard procedure 2 was followed by use of compound 18a (0.124 g, 0.50 mmol, 1.0 equiv) and 1-(2-chloroethyl)pyrrolidine (0.0802 g, 0.60 mmol, 1.2 equiv) in acetonitrile (15 mL) to which potassium carbonate (0.200 g, 1.45 mmol, 2.9 equiv) was added. After workup and purification with column chromatography (75% EtOAc in hexane as eluent), compound 20a (0.110 g, 0.32 mmol) was obtained in 64% yield as yellow liquid: 1H-NMR (acetone-d6, 500 MHz) δ 1.65 (br s, 4 H, NCH2CH2), 2.52 (br s, 4 H, NCH2CH2), 2.84 (t, J = 6.5 Hz, 2 H, pyrrolidine-CH2CH2), 4.83 (t, J = 6.5 Hz, 2 H, piperidine-CH2CH2), 7.37 (t, J = 7.5 Hz, 1H, ArCH), 7.46 (t, J = 7.5 Hz, 1H, ArCH), 7.50 (d, J = 6.5 Hz, 3 H, 3 × ArCH), 7.69 (d, J = 8.0 Hz, 1 H, ArCH), 7.83 (d, J = 8.0 Hz, 2 H, 2 × ArCH), 7.85 (d, J = 8.0 Hz, 1 H, ArCH), 7.90 (d, J = 16.0 Hz, 1 H, COCH), 8.24 (d, J = 16.0 Hz, 1 H, PhCH); 13C-NMR (acetone-d6, 125 MHz) δ 24.25, 44.80, 54.78, 56.07, 112.24, 122.38, 124.36, 124.41, 126.64, 129.65, 130.00, 131.71, 135.79, 137.55, 142.76, 144.58, 148.35, 183.25; IR (neat) 3650 (s), 3359 (s), 2920 (s), 2850 (s), 2363 (m), 1660 (C=O, s), 1632 (s), 1602 (C=N, s), 1470 (s), 1333 (C–N, s), 1243 (m), 1166 (m), 1040 (s), 745 (s), 697 (m); MS (ESI) m/z calcd for C22H23N3O: 345.1841, found: 345.1842.
(2E)-1-(1-(2-(Pyrrolidin-1-yl)ethyl)-1H-benzimidazol-2-yl)-3-(4-methylphenyl)-2-propen-1-one (20b). Standard procedure 2 was followed by use of compound 18b (0.131 g, 0.50 mmol, 1.0 equiv) and 1-(2-chloroethyl)pyrrolidine (0.0802 g, 0.60 mmol, 1.2 equiv) in acetonitrile (15 mL) to which potassium carbonate (0.200 g, 1.45 mmol, 2.9 equiv) was added. After workup and purification with column chromatography (60% EtOAc in hexane as eluent), compound 20b (0.134 g, 0.37 mmol) was obtained in 74% yield as brown oil: 1H-NMR (acetone-d6, 500 MHz) δ 1.65 (p, J = 3.5 Hz, 4 H, NCH2CH2), 2.40 (s, 3 H, PhCH3), 2.52 (br s, 4 H, NCH2CH2), 2.85 (t, J = 6.5 Hz, 2 H, pyrrolidine-CH2CH2), 4.85 (t, J = 6.5 Hz, 2 H, pyrrolidine-CH2CH2), 7.33 (d, J = 7.5 Hz, 2 H, 2 × ArCH), 7.37 (t, J = 7.5 Hz, 1 H, ArCH), 7.46 (t, J = 7.5 Hz, 1 H, ArCH), 7.70 (d, J = 8.5 Hz, 1 H, ArCH), 7.73 (d, J = 8.0 Hz, 2 H, 2 × ArCH), 7.85 (d, J = 8.0 Hz, 1 H, ArCH), 7.88 (d, J = 16.0 Hz, 1 H, COCH), 8.23 (d, J = 16.0 Hz, 1 H, PhCH); 13C-NMR (acetone-d6, 125 MHz) δ 21.59, 24.39, 45.13, 54.98, 56.45, 112.26, 122.46, 123.63, 124.27, 126.42, 129.74, 130.77, 133.26, 137.73, 142.28, 143.00, 144.55, 148.82, 183.48; IR (neat) 3775 (m), 3357 (m), 2967 (m), 2879 (s), 2785 (m), 2291 (m), 1705 (s), 1660 (C=O, s), 1596 (C=N, s), 1476 (w), 1449 (w), 1407 (w), 1366 (m), 1327 (C–N, m) 1231 (s), 1037 (m), 982 (w), 894 (w), 812 (s), 755 (m), 741 (m), 727 (w); MS (ESI) m/z calcd for C23H25N3O: 359.1997, found: 359.1998.
(2E)-3-(4-Methoxyphenyl)-1-(1-(2-(pyrrolidin-1-yl)ethyl)-1H-benzimidazol-2-yl)-2-propen-1-one (20c). Standard procedure 2 was followed by use of compound 18c (0.139 g, 0.50 mmol, 1.0 equiv) and 1-(2-chloroethyl)pyrrolidine (0.0802 g, 0.60 mmol, 1.2 equiv) in acetonitrile (15 mL) to which potassium carbonate (0.200 g, 1.45 mmol, 2.9 equiv) was added. After workup and purification with column chromatography (75% EtOAc in hexane as eluent), compound 20c (0.125 g, 0.33 mmol) was obtained in 66% yield as brown oil: 1H-NMR (acetone-d6, 500 MHz) δ 1.65 (p, J = 3.0 Hz, 4 H, NCH2CH2), 2.53 (br s, 4 H, NCH2CH2), 2.85 (t, J = 6.5 Hz, 2 H, pyrrolidine-CH2CH2), 3.87 (s, 3 H, OCH3), 4.84 (t, J = 6.5 Hz, 2 H, pyrrolidine-CH2CH2), 7.05 (d, J = 8.5 Hz, 2 H, 2 × ArCH), 7.36 (t, J = 7.5 Hz, 1 H, ArCH), 7.45 (t, J = 7.5 Hz, 1 H, ArCH), 7.68 (d, J = 8.0 Hz, 1 H, ArCH), 7.79 (d, J = 9.0 Hz, 2 H, 2 × ArCH), 7.84 (d, J = 9.5 Hz, 1 H, ArCH), 7.87 (d, J = 16.0 Hz, 1 H, COCH), 8.10 (d, J = 16.0 Hz, 1H, PhCH); 13C-NMR (acetone-d6, 125 MHz) δ 24.36, 45.06, 54.94, 55.94, 56.93, 112.21, 115.52, 122.16, 122.39, 124.23, 126.33, 128.54, 131.57, 137.67, 142.95, 144.48, 148.83, 163.12, 183.38; IR (neat) 3846 (s), 3736 (s), 3650 (m), 3358 (s), 2921 (s), 2851 (s), 2368 (m), 1661 (C=O, s), 1590 (C=N, s), 1511 (s), 1458 (s), 1333 (C–N, s), 1289 (m), 1254 (s), 1171 (s), 1036 (s), 829 (s), 744 (s); MS (ESI) m/z calcd for C23H25N3O2: 375.1947, found: 375.1948.
(2E)-3-(4-Chlorophenyl)-1-(1-(2-(pyrrolidin-1-yl)ethyl)-1H-benzimidazol-2-yl)-2-propen-1-one (20d). Standard procedure 2 was followed by use of compound 18d (0.141 g, 0.50 mmol, 1.0 equiv) and 1-(2-chloroethyl)pyrrolidine (0.0802 g, 0.60 mmol, 1.2 equiv) in acetonitrile (15 mL) to which potassium carbonate (0.200 g, 1.45 mmol, 2.9 equiv) was added. After workup and purification with column chromatography (80% EtOAc in hexane as eluent), compound 20d (0.094 g, 0.25 mmol) was obtained in 50% yield as brown oil: 1H-NMR (acetone-d6, 500 MHz) δ 1.67 (p, J = 3.0 Hz, 4 H, NCH2CH2), 2.56 (br s, 4 H, NCH2CH2), 2.87 (t, J = 6.5 Hz, 2H, pyrrolidine-CH2CH2), 4.85 (t, J = 6.5 Hz, 2 H, pyrrolidine-CH2CH2), 7.38 (t, J = 7.5 Hz, 1 H, ArCH), 7.47 (t, J = 8.0 Hz, 1 H, ArCH), 7.54 (d, J = 8.0 Hz, 2 H, 2 × ArCH), 7.71 (d, J = 8.5 Hz, 1 H, ArCH), 7.85 (d, J = 8.5 Hz, 1 H, ArCH), 7.87 (d, J = 8.5 Hz, 2 H, 2 × ArCH), 7.88 (d, J = 16.0 Hz, 1 H, ketone-CH), 8.23 (d, J = 16.0 Hz, 1 H, ph-CH); 13C-NMR (acetone-d6, 125 MHz) δ 24.36, 45.09, 54.96, 56.39, 112.33, 122.49, 124.40, 125.27, 126.60, 130.21, 131.24, 134.81, 136.95, 140.46, 142.92, 183.23; IR (neat) 3352 (s), 2995 (m), 2912 (m), 2307 (m), 1759 (s), 1654 (C=O, s), 1629 (C=N, s), 1465 (m), 1331 (C–N, w), 1246 (s), 1009 (s); MS (ESI) m/z calcd for C22H22ClN3O: 379.1451, found: 379.1450.
(2E)-3-Phenyl-1-(1-(2-(piperidin-1-yl)ethyl)-1H-benzimidazol-2-yl)-2-propen-1-one (21a). Standard procedure 2 was followed by use of compound 18a (0.124 g, 0.50 mmol, 1.0 equiv) and 1-(2-chloroethyl)piperidine (0.0886 g, 0.60 mmol, 1.2 equiv) in acetonitrile (15 mL) to which potassium carbonate (0.200 g, 1.45 mmol, 2.9 equiv) was added. After workup and purification with column chromatography (33% EtOAc in hexane as eluent), compound 21a (0.120 g, 0.33 mmol) was obtained in 66% yield as yellow liquid: 1H-NMR (acetone-d6, 500 MHz) δ 1.33 (p, J = 5.5 Hz, 2 H, NCH2CH2CH2), 1.42 (p, J = 5.5 Hz, 4 H, NCH2CH2CH2), 2.40 (br s, 4 H, NCH2CH2CH2), 2.66 (t, J = 6.5 Hz, 2 H, piperidine-CH2CH2), 4.81 (t, J = 6.5 Hz, 2 H, piperidine-CH2CH2), 7.36 (dd, J = 7.5 Hz, 1 H, ArCH), 7.45 (dd, J = 7.5 Hz, 1 H, ArCH), 7.49 (d, J = 7.0 Hz, 3 H, 3 × ArCH), 7.67 (d, J = 8.0 Hz, 1 H, ArCH), 7.82 (d, J = 8.0 Hz, 2 H, 2 × ArCH), 7.85 (d, J = 8.0 Hz, 1 H, ArCH), 7.89 (d, J = 16.0 Hz, 1 H, COCH), 8.28 (d, J = 16.0 Hz, 1 H, PhCH); 13C-NMR (acetone-d6, 125 MHz) δ 25.03, 26.83, 43.84, 55.67, 59.39, 112.36, 122.44, 124.27, 124.55, 126.42, 129.62, 130.00, 131.65, 135.89, 137.76, 142.87, 144.41, 148.51, 183.32; IR (neat) 3786 (w), 3357 (m), 3055 (w), 3022 (w), 2879 (s), 2775 (w), 2302 (w), 1758 (s), 1662 (C=O, s), 1599 (C=N, s), 1476 (s), 1448 (m), 1407 (m), 1369 (m), 1330 (C–N, s), 1240 (s), 1161 (m), 1117 (w), 1043 (s), 982 (s), 894 (w), 744 (s), 697 (s); MS (ESI) m/z calcd for C23H25N3O: 359.1998, found: 359.1996.
(2E)-1-(1-(2-(Piperidin-1-yl)ethyl)-1H-benzimidazol-2-yl)-3-(4-methylphenyl)-2-propen-1-one (21b). Standard procedure 2 was followed by use of compound 18b (0.131 g, 0.50 mmol, 1.0 equiv) and 1-(2-chloroethyl)piperidine (0.0886 g, 0.60 mmol, 1.2 equiv) in acetonitrile (15 mL) to which potassium carbonate (0.200 g, 1.45 mmol, 2.9 equiv) was added. After workup and purification with column chromatography (33% EtOAc in hexane as eluent), compound 21b (0.110 g, 0.29 mmol) was obtained in 58% yield as brown oil: 1H-NMR (acetone-d6, 500 MHz) δ 1.43 (p, J = 5.5 Hz, 6 H, NCH2CH2CH2 and NCH2CH2CH2), 2.40 (s, 4 H, NCH2CH2CH2), 2.41 (s, 3 H, ph-CH3), 2.67 (t, J = 6.5 Hz, 2 H, piperidin-CH2CH2), 4.84 (t, J = 6.5 Hz, 2 H, piperidin-CH2CH2), 7.32 (d, J = 7.5 Hz, 2 H, 2 × ArCH), 7.37 (t, J = 7.5 Hz, 1H, ArCH), 7.45 (t, J = 7.5 Hz, 1 H, ArCH), 7.69 (d, J = 8.5 Hz, 1 H, ArCH), 7.72 (d, J = 8.0 Hz, 2 H, 2 × ArCH), 7.84 (d, J = 8.0 Hz, 1 H, ArCH), 7.88 (d, J = 16.0 Hz, 1 H, COCH), 8.23 (d, J = 16.0 Hz, 1H, PhCH); 13C-NMR (acetone-d6, 125 MHz) δ 21.59, 25.07, 26.86, 43.86, 55.72, 59.45, 112.41, 122.43, 123.63, 124.25, 126.36, 129.72, 130.74, 133.26, 137.81, 142.23, 142.94, 144.58, 148.70, 183.47; IR (neat) 3788 (s), 3663 (w), 3574 (w), 3355 (s), 2918 (s), 2846 (s), 1764 (w), 1657 (C=O, w), 1632 (w), 1596 (C=N, w), 1465 (w), 1246 (w), 1325 (C–N, w), 1300 (w), 1246 (s), 988 (w); MS (ESI) m/z calcd for C24H27N3O: 373.2154, found: 373.2152.
(2E)-3-(4-methoxyphenyl)-1-(1-(2-(piperidin-1-yl)ethyl)-1H-benzimidazol-2-yl)-2-propen-1-one (21c). Standard procedure 2 was followed by use of compound 18c (0.139 g, 0.50 mmol, 1.0 equiv) and 1-(2-chloroethyl)piperidine (0.0886 g, 0.60 mmol, 1.2 equiv) in acetonitrile (15 mL) to which potassium carbonate (0.200 g, 1.45 mmol, 2.9 equiv) was added. After workup and purification with column chromatography (40% EtOAc in hexane as eluent), compound 21c (0.166 g, 0.43 mmol) was obtained in 86% yield as brown oil: 1H-NMR (acetone-d6, 500 MHz) δ 1.33 (p, J = 5.5 Hz, 2 H, NCH2CH2CH2), 1.42 (p, J = 5.5 Hz, 4 H, NCH2CH2CH2), 2.41 (s, 4 H, NCH2CH2CH2), 2.67 (t, J = 6.5 Hz, 2 H, piperidin-CH2CH2), 3.89 (s, 3 H, OCH3), 4.84 (t, J = 6.5 Hz, 2 H, piperidin-CH2CH2), 7.06 (d, J = 8.5 Hz, 2 H, 2 × ArCH), 7.36 (t, J = 7.5 Hz, 1 H, ArCH), 7.45 (t, J = 7.5 Hz, 1 H, ArCH), 7.69 (d, J = 8.0 Hz, 1 H, ArCH), 7.80 (d, J = 9.0 Hz, 2 H, 2 × ArCH), 7.84 (d, J = 8.0 Hz, 1 H, ArCH), 7.87 (d, J = 16.0 Hz, 1 H, ketone-CH), 8.14 (d, J = 16.0 Hz, 1 H, ph-CH); 13C-NMR (acetone-d6, 125 MHz) δ 25.09, 26.89, 43.87, 55.74, 55.95, 112.40, 115.55, 122.24, 122.38, 124.19, 126.26, 128.59, 131.56, 137.81, 142.96, 144.47, 148.85, 163.14, 183.45; IR (neat) 3780 (s), 3357 (s), 3187 (s), 2921 (s), 2851 (s), 1661 (C=O, s), 1630 (s), 1591 (C=N, s), 1569 (m), 1512 (s), 1473 (m), 1331 (C–N, s), 1255 (s), 1172 (s), 1040 (s), 829 (m), 740 (m); MS (ESI) m/z calcd for C24H27N3O2: 389.2103, found: 389.2103.
(2E)-3-(4-Chlorophenyl)-1-(1-(2-(piperidin-1-yl)ethyl)-1H-benzimidazol-2-yl)-2-propen-1-one (21d). Standard procedure 2 was followed by use of compound 18d (0.141 g, 0.50 mmol, 1.0 equiv) and 1-(2-chloroethyl)piperidine (0.0886 g, 0.60 mmol, 1.2 equiv) in acetonitrile (15 mL) to which potassium carbonate (0.200 g, 1.45 mmol, 2.9 equiv) was added. After workup and purification with column chromatography (25% EtOAc in hexane as eluent), compound 21d (0.098 g, 0.25 mmol) was obtained in 50% yield as yellow solids: 1H-NMR (acetone-d6, 500 MHz) δ 1.33 (p, J = 5.5 Hz, 2 H, NCH2CH2CH2), 1.42 (p, J = 5.5 Hz, 4 H, NCH2CH2CH2), 2.41 (s, 4 H, NCH2CH2CH2), 2.67 (t, J = 6.5 Hz, 2 H, piperidin-CH2CH2), 4.85 (t, J = 6.5 Hz, 2 H, piperidin-CH2CH2), 7.38 (t, J = 7.5 Hz, 1 H, ArCH), 7.47 (t, J = 7.5 Hz, 1 H, ArCH), 7.55 (d, J = 8.5 Hz, 2 H, 2 × ArCH), 7.71 (d, J = 8.5 Hz, 1 H, ArCH), 7.85 (d, J = 8.5 Hz, 1 H, ArCH), 7.88 (d, J = 9.0 Hz, 2 H, 2 × ArCH), 7.88 (d, J = 16.0 Hz, 1 H, COCH), 8.27 (d, J = 16.0 Hz, 1 H, PhCH); 13C-NMR (acetone-d6, 125 MHz) δ 25.06, 26.87, 43.89, 55.72, 59.44, 112.49, 122.48, 124.36, 125.33, 126.54, 130.20, 131.22, 134.83, 136.93, 137.85, 142.92, 148.55, 183.28; IR (neat) 3840 (s), 3736 (s), 3650 (m), 3568 (m), 3363 (s), 2921 (s), 2851 (s), 2362 (s), 1666 (C=O, s), 1604 (C=N, s), 1490 (s), 1405 (s), 1334 (C–N, s), 1300 (m), 1164 (s), 1093 (s), 1043 (s), 894 (w), 822 (s), 741 (s), 497 (m); MS (ESI) m/z calcd for C23H24ClN3O: 393.1608, found: 393.1609.
(2E)-1-(1-(2-Morpholinoethyl)-1H-benzimidazol-2-yl)-3-phenyl-2-propen-1-one (22a). Standard procedure 2 was followed by use of compound 18a (0.124 g, 0.50 mmol, 1.0 equiv) and 4-(2-chloroethyl)morpholine (0.0898 g, 0.60 mmol, 1.2 equiv) in acetonitrile (15 mL) was added potassium carbonate (0.200 g, 1.45 mmol, 2.9 equiv). After workup and purification with column chromatography (33% EtOAc in hexane as eluent), compound 22a (0.152 g, 0.42 mmol) was obtained in 84% yield as yellow solids: 1H-NMR (acetone-d6, 500 MHz) δ 2.46 (t, J = 5.0 Hz, 4 H, NCH2CH2O), 2.74 (t, J = 6.5 Hz, 2 H, morpholine-CH2CH2), 3.50 (t, J = 5.0 Hz, 4 H, OCH2), 4.88 (t, J = 6.5 Hz, 2 H, morpholine-CH2CH2), 7.38 (t, J = 7.5 Hz, 1 H, ArCH), 7.47 (t, J = 7.5 Hz, 1 H, ArCH), 7.51 (d, J = 7.0 Hz, 3 H, 3 × ArCH), 7.72 (d, J = 8.0 Hz, 1 H, ArCH), 7.85 (d, J = 7.0 Hz, 2 H, 2 × ArCH), 7.86 (d, J = 7.0 Hz, 1 H, ArCH), 7.91 (d, J = 16.0 Hz, 1 H, COCH), 8.29 (d, J = 16.0 Hz, 1 H, PhCH); 13C-NMR (acetone-d6, 125 MHz) δ 43.43, 54.86, 59.12, 67.47, 112.41, 122.47, 124.41, 124.55, 126.57, 129.71, 130.08, 131.79, 135.88, 137.71, 142.86, 144.72, 148.59, 183.52; IR (neat) 3786 (s), 3352 (s), 2921 (s), 2851 (s), 2308 (w), 1665 (C=O, s), 1602 (C=N, s), 1446 (br), 1410 (m), 1333 (C–N, s), 1300 (m), 1116 (s), 1067 (m), 1040 (m), 867 (w), 744 (s), 554 (w); MS (ESI) m/z calcd for C22H23N3O2: 361.1790, found: 361.1792.
(2E)-1-(1-(2-Morpholinoethyl)-1H-benzimidazol-2-yl)-3-(4-methylphenyl)-2-propen-1-one (22b). Standard procedure 2 was followed by use of compound 18b (0.131 g, 0.50 mmol, 1.0 equiv) and 4-(2-chloroethyl)morpholine (0.0898 g, 0.60 mmol, 1.2 equiv) in acetonitrile (15 mL) to which potassium carbonate (0.200 g, 1.45 mmol, 2.9 equiv) was added. After workup and purification with column chromatography (33% EtOAc in hexane as eluent), compound 22b (0.119 g, 0.32 mmol) was obtained in 64% yield as brown oil: 1H-NMR (acetone-d6, 500 MHz) δ 2.39 (s, 3 H, PhCH3), 2.45 (t, J = 5.0 Hz, 4 H, NCH2CH2O), 2.72 (t, J = 6.5 Hz, 2 H, morpholine-CH2CH2), 3.50 (t, J = 5.0 Hz, 4 H, OCH2), 4.86 (t, J = 6.5 Hz, 2 H, morpholine-CH2CH2), 7.32 (d, J = 8.0 Hz, 2 H, 2 × ArCH), 7.37 (t, J = 7.5 Hz, 1 H, ArCH), 7.46 (t, J = 7.5 Hz, 1 H, ArCH), 7.70 (d, J = 8.0 Hz, 1 H, ArCH), 7.72 (d, J = 8.0 Hz, 2 H, 2 × ArCH), 7.85 (d, J = 7.0 Hz, 1 H, ArCH), 7.87 (d, J = 16.0 Hz, 1 H, COCH), 8.23 (d, J = 16.0 Hz, 1H, PhCH); 13C-NMR (acetone-d6, 125 MHz) δ 21.59, 43.42, 54.87, 59.15, 67.48, 112.35, 122.46, 123.58, 124.32, 126.45, 129.75, 130.74, 133.19, 137.71, 142.30, 142.90, 144.72, 148.66, 183.54; IR (neat) 3786 (s), 3357 (s), 3187 (m), 2920 (s), 2846 (s), 1660 (C=O, s), 1632 (m), 1597 (C=N, s), 1470 (br), 1410 (m), 1333 (C–N, s), 1303 (m), 1180 (m), 1114 (s), 985 (m), 867 (m), 815 (m), 744 (s); MS (ESI) m/z calcd for C23H25N3O2: 375.1947, found: 375.1948.
(2E)-3-(4-Methoxyphenyl)-1-(1-(2-morpholinoethyl)-1H-benzimidazol-2-yl)-2-propen-1-one (22c). Standard procedure 2 was followed by use of compound 18c (0.139 g, 0.50 mmol, 1.0 equiv) and 4-(2-chloroethyl)morpholine (0.0898 g, 0.60 mmol, 1.2 equiv) in acetonitrile (15 mL) to which potassium carbonate (0.200 g, 1.45 mmol, 2.9 equiv) was added. After workup and purification with column chromatography (33% EtOAc in hexane as eluent), compound 22c (0.122 g, 0.31 mmol) was obtained in 62% yield as yellow oil: 1H-NMR (acetone-d6, 500 MHz) δ 2.45 (t, J = 5.0 Hz, 4 H, NCH2CH2O), 2.72 (t, J = 6.5 Hz, 2 H, morpholine-CH2CH2), 3.50 (t, J = 5.0 Hz, 4 H, OCH2), 3.88 (s, 3 H, OCH3), 4.86 (t, J = 6.5 Hz, 2 H, morpholine-CH2CH2), 7.05 (d, J = 8.0 Hz, 2 H, 2 × ArCH), 7.37 (t, J = 7.5 Hz, 1H, ArCH), 7.45 (t, J = 7.5 Hz, 1 H, ArCH), 7.68 (d, J = 8.5 Hz, 1 H, ArCH), 7.79 (d, J = 8.5 Hz, 2 H, 2 × ArCH), 7.85 (d, J = 8.5 Hz, 1 H, ArCH), 7.87 (d, J = 16.0 Hz, 1 H, COCH), 8.15 (d, J = 16.0 Hz, 1H, PhCH); 13C-NMR (acetone-d6, 125 MHz) δ 43.40, 43.86, 55.93, 59.14, 67.47, 112.28, 115.50, 122.15, 122.40, 124.25, 126.33, 128.49, 131.57, 137.67, 142.88, 144.60, 148.75, 163.11, 183.54; IR (neat) 3789 (s), 3663 (w), 3359 (br), 2922 (s), 2851 (s), 1645 (C=O, s), 1591 (C=N,s), 1512 (w), 1468 (br), 1333 (C–N, m), 1289 (m), 1256 (s), 1173 (s), 1114 (s), 1067 (m), 1040 (m), 829 (w), 741 (w); MS (ESI) m/z calcd for C23H25N3O3: 391.1896, found: 391.1897.
(2E)-3-(4-Chlorophenyl)-1-(1-(2-morpholinoethyl)-1H-benzimidazol-2-yl)-2-propen-1-one (22d). Standard procedure 2 was followed by use of compound 18d (0.141 g, 0.50 mmol, 1.0 equiv) and 4-(2-chloroethyl)morpholine (0.0898 g, 0.60 mmol, 1.2 equiv) in acetonitrile (15 mL) to which potassium carbonate (0.200 g, 1.45 mmol, 2.9 equiv) was added. After workup and purification with column chromatography (25% EtOAc in hexane as eluent), compound 22d (0.123 g, 0.31 mmol) was obtained in 62% yield as yellow solids: 1H-NMR (acetone-d6, 500 MHz) δ 2.45 (t, J = 5.0 Hz, 4 H, NCH2CH2O), 2.73 (t, J = 6.5 Hz, 2 H, morpholine-CH2CH2), 3.49 (t, J = 5.0 Hz, 4 H, OCH2), 4.88 (t, J = 6.5 Hz, 2 H, morpholine-CH2CH2), 7.38 (t, J = 7.5 Hz, 1 H, ArCH), 7.47 (t, J = 7.5 Hz, 1 H, ArCH), 7.54 (d, J = 8.5 Hz, 2 H, 2 × ArCH), 7.72 (d, J = 8.0 Hz, 1 H, ArCH), 7.85 (d, J = 8.0 Hz, 1 H, ArCH), 7.88 (d, J = 8.5 Hz, 2 H, 2 × ArCH), 7.88 (d, J = 16.0 Hz, 1 H, COCH), 8.28 (d, J = 16.0 Hz, 1 H, PhCH); 13C-NMR (acetone-d6, 125 MHz) δ 43.48, 54.91, 59.17, 67.51, 112.44, 122.54, 124.42, 125.31, 126.62, 130.21, 131.25, 134.79, 136.98, 137.80, 142.95, 143.04, 148.53, 183.36; IR (neat) 3357 (s), 3192 (s), 2920 (s), 2850 (s), 1662 (C=O, s), 1632 (s), 1602 (C=N, s), 1564 (m), 1489 (m), 1470 (s), 1410 (s), 1333 (C–N, s) 1300(s), 1114 (s), 1034 (s), 1010 (s), 823 (s), 740 (s); MS (ESI) m/z calcd for C22H22ClN3O2: 395.1401, found: 395.1403.
(2E)-1-(1-(3-Morpholinopropyl)-1H-benzimidazol-2-yl)-3-phenyl-2-propen-1-one (23a). Standard procedure 2 was followed by use of compound 18a (0.124 g, 0.50 mmol, 1.0 equiv) and 4-(3-chloropropyl)morpholine (0.0982 g, 0.60 mmol, 1.2 equiv) in acetonitrile (15 mL) to which potassium carbonate (0.200 g, 1.45 mmol, 2.9 equiv) was added. After workup and purification with column chromatography (66.7% EtOAc in hexane as eluent), compound 23a (0.116 g, 0.31 mmol) was obtained in 62% yield as yellow oil: 1H-NMR (acetone-d6, 500 MHz) δ 2.30 (s, 6 H, NCH2CH2O and morpholine-CH2CH2CH2), 2.33 (m, 8 H, morpholine-CH2CH2CH2), 3.57 (t, J = 4.5 Hz, 4 H, OCH2), 4.79 (t, J = 7.0 Hz, 2 H, morpholine-CH2CH2CH2), 7.37 (t, J = 7.5 Hz, 1 H, ArCH), 7.45 (t, J = 7.5 Hz, 1 H, ArCH), 7.49 (d, J = 7.0 Hz, 3 H, 3 × ArCH), 7.75 (d, J = 8.0 Hz, 1 H, ArCH), 7.81 (d, J = 8.0 Hz, 1 H, ArCH), 7.82 (d, J = 8.0 Hz, 1 H, ArCH), 7.86 (d, J = 7.0 Hz, 1 H, ArCH), 7.88 (d, J = 16.0 Hz, 1 H, COCH), 8.33 (d, J = 16.0 Hz, 1 H, PhCH); 13C-NMR (acetone-d6, 125 MHz) δ 27.45, 44.26, 54.32, 56.24, 67.27, 112.39, 122.38, 124.24, 124.39, 126.58, 129.64, 129.96, 131.68, 135.75, 137.78, 142.64, 144.48, 147.85, 183.09; IR (neat) 3780 (s), 3687 (s), 2918 (w), 2846 (w), 2808 (w), 1770 (m), 1663 (C=O, s), 1599 (C=N, s), 1476 (m), 1457 (w), 1407 (m), 1333 (C–N, s), 1306 (m), 1117 (s), 1043 (m), 979 (m), 859 (m), 744 (s), 686 (m); MS (ESI) m/z calcd for C23H25N3O2: 375.1947, found: 375.1944.
(2E)-1-(1-(3-Morpholinopropyl)-1H-benzimidazol-2-yl)-3-(4-methylphenyl)-2-propen-1-one (23b). Standard procedure 2 was followed by use of compound 18b (0.131 g, 0.50 mmol, 1.0 equiv) and 4-(3-chloropropyl)morpholine (0.0982 g, 0.60 mmol, 1.2 equiv) in acetonitrile (15 mL) to which potassium carbonate (0.200 g, 1.45 mmol, 2.9 equiv) was added. After workup and purification with column chromatography (40% EtOAc in hexane as eluent), compound 23b (0.108 g, 0.28 mmol) was obtained in 56% yield as brown oil: 1H-NMR (acetone-d6, 500 MHz) δ 2.30 (m, 6 H, NCH2CH2O and morpholine-CH2CH2CH2), 2.35 (t, J = 6.5 Hz, 2 H, morpholine-CH2CH2CH2), 2.39 (s, 3 H, ph-CH3), 3.57 (t, J = 4.5 Hz, 4 H, OCH2), 4.81 (t, J = 7.5 Hz, 2 H, morpholine-CH2CH2CH2), 7.32 (d, J = 8.0 Hz, 2 H, 2 × ArCH), 7.37 (t, J = 7.0 Hz, 1 H, ArCH), 7.46 (t, J = 7.5 Hz, 1 H, ArCH), 7.70 (d, J = 8.0 Hz, 2 H, 2 × ArCH), 7.76 (d, J = 8.0 Hz, 1 H, ArCH), 7.86 (d, J = 8.0 Hz, 1 H, ArCH), 7.86 (d, J = 16.0 Hz, 1 H, COCH), 8.28 (d, J = 16.0 Hz, 1 H, PhCH); 13C-NMR (acetone-d6, 125 MHz) δ 21.59, 27.57, 44.28, 54.47, 56.33, 67.40, 112.45, 122.46, 123.41, 124.33, 126.48, 129.74, 130.72, 133.20, 137.94, 142.26, 142.84, 144.50, 148.12, 183.26; IR (neat) 3840 (s), 3736 (s), 3673 (m), 3650 (m), 3568 (m), 2921 (s), 2851 (s), 2348 (s), 1663 (C=O, s), 1600 (C=N, s), 1454 (w), 1407 (w), 1333 (C–N, s), 1180 (s), 1117 (s), 1036 (s), 985 (w), 814 (s), 742 (m); MS (ESI) m/z calcd for C24H27N3O2: 389.2103, found: 389.2101.
(2E)-3-(4-Methoxyphenyl)-1-(1-(3-morpholinopropyl)-1H-benzimidazol-2-yl)-2-propen-1-one (23c). Standard procedure 2 was followed by use of compound 18c (0.139 g, 0.50 mmol, 1.0 equiv) and 4-(3-chloropropyl)morpholine (0.0982 g, 0.60 mmol, 1.2 equiv) in acetonitrile (15 mL) to which potassium carbonate (0.200 g, 1.45 mmol, 2.9 equiv) was added. After workup and purification with column chromatography (50% EtOAc in hexane as eluent), compound 23c (0.174 g, 0.43 mmol) was obtained in 86% yield as yellow oil: 1H-NMR (acetone-d6, 500 MHz) δ 2.31–2.34 (m, 8 H, NCH2CH2O, morpholine-CH2CH2CH2 and morpholine-CH2CH2CH2), 3.57 (t, J = 4.5 Hz, 4 H, OCH2), 3.87(s, 3 H, OCH3), 4.80 (t, J = 7.5 Hz, 2 H, morpholine-CH2CH2CH2), 7.04 (d, J = 8.5 Hz, 2 H, 2 × ArCH), 7.36 (t, J = 7.5 Hz, 1 H, ArCH), 7.44 (t, J = 7.5 Hz, 1 H, ArCH), 7.74 (d, J = 8.0 Hz, 1 H, ArCH), 7.78 (d, J = 9.0 Hz, 1 H, ArCH), 7.84 (d, J = 8.0 Hz, 1 H, ArCH), 7.85 (d, J = 16.0 Hz, 1 H, COCH), 8.18 (d, J = 16.0 Hz, 1 H, PhCH); 13C-NMR (acetone-d6, 125 MHz) δ 29.62, 44.26, 54.50, 55.94, 56.34, 67.43, 112.41, 115.51, 122.03, 122.43, 124.24, 126.34, 128.54, 131.57, 137.96, 142.89, 144.36, 148.27, 163.11, 183.21; IR (neat) 3359 (s), 3186 (s), 2921 (s), 2851 (s), 1660 (C=O, s), 1632 (s), 1591 (C=N,s), 1511 (s), 1471 (s), 1418 (w), 1410 (w), 1331 (C–N, s), 1255 (s), 1172 (s), 1116 (s), 1036 (s), 828 (s), 741 (s); MS (ESI) m/z calcd for C24H27N3O3: 405.2052, found: 405.2051.
(2E)-3-(4-Chlorophenyl)-1-(1-(3-morpholinopropyl)-1H-benzimidazol-2-yl)-2-propen-1-one (23d). Standard procedure 2 was followed by use of compound 18d (0.141 g, 0.50 mmol, 1.0 equiv) and 4-(3-chloropropyl)morpholine (0.0982 g, 0.60 mmol, 1.2 equiv) in acetonitrile (15 mL) to which potassium carbonate (0.200 g, 1.45 mmol, 2.9 equiv) was added. After workup and purification with column chromatography (33% EtOAc in hexane as eluent), compound 23d (0.115 g, 0.28 mmol) was obtained in 56% yield as brown oil: 1H-NMR (acetone-d6, 500 MHz) δ 2.32 (br s, 4 H, NCH2CH2O), 2.36 (t, J = 6.5 Hz, 2 H, morpholine-CH2CH2CH2), 2.67 (s, 2 H, morpholine-CH2CH2CH2), 3.57 (t, J = 4.5 Hz, 4 H, OCH2), 4.80 (t, J = 7.0 Hz, 2H, morpholine- CH2CH2CH2), 7.38 (t, J = 7.5 Hz, 1 H, ArCH), 7.46 (t, J = 7.5 Hz, 1H, ArCH), 7.54 (d, J = 8.5 Hz, 2H, 2 × ArCH), 7.78 (d, J = 8.0 Hz, 1H, ArCH), 7.86 (d, J = 8.0 Hz, 1H, ArCH), 7.87 (d, J = 8.5 Hz, 2H, 2 × ArCH), 7.88 (d, J = 16.0 Hz, 1H, ketone-CH), 8.32 (d, J = 16.0 Hz, 1H, ph-CH); 13C-NMR (acetone-d6, 125 MHz) δ 27.56, 44.33, 54.50, 56.35, 67.42, 112.55, 122.55, 124.44, 125.16, 126.66, 128.94, 129.61, 130.20, 131.24, 134.81, 138.03, 142.84, 148.01, 183.11; IR (neat) 3788 (s), 3663 (w), 3573 (w), 3359 (m), 2920 (s), 2850 (s), 1665 (C=O, s), 1602 (C=N, s), 1407 (w), 1334 (C–N, s), 1300 (w), 1117 (s), 1092 (s), 1043 (s), 821 (s), 742 (s); MS (ESI) m/z calcd for C23H24ClN3O2: 409.1557, found: 409.1557.

3.4. MTT Assay

Assays to measure proliferation, viability, and cytotoxicity are commonly used to monitor the response and health of cells in culture after treatment with various stimuli. First, cell-lines were seeded in 96 well plates with 3000–5000 cells/well. On the second day, cell-lines were treated with different compounds in various concentrations of 100, 50, 25, 12.5, 6.25, 0 µg/µL. After 48 h, the MTT reagent 5(6)-carboxyfluorescein diacetate N-succinimidyl ester (CFSE) (5 mg/mL) was diluted to one tenth in culture medium as the MTT assay solution. After the addition of MTT assay solution in 96-well plates (100 µL/well), the sample was incubated at 37 °C for 1–1.5 h. Cell counting using viability dyes such as trypan blue or calcein-AM can provide both the rate of proliferation as well as the percentage of viable cells. CFSE is a popular choice for measuring the number of cellular divisions a population has undergone. Upon entering the cell, CFSE is cleaved by intracellular esterases to form the fluorescent compound and the succinimidyl ester group covalently reacts with primary amines on intracellular proteins. After labeling the cells with MTT, as described above, all but 100 µL of medium was removed from the wells. Then DMSO (50 µL) was added to each well and mixed thoroughly with a pipette and incubated at 37 °C for 10 min. After incubation, the sample was gently shaken and absorbance was read at 560 nm by an ELISA reader.

3.5. Flow Cytometry

Cell cycle analysis of compounds 20a–23a was performed by propidium iodide (PI) staining followed by flow cytometry in OVCAR-3 cells. OVCAR-3 cells were seeded into 6-cm dishes at a density of 2 × 105 cells/dish for 24 h. Cells were then treated with serial dilution of compound 20a–23a for 48 h. The cells were harvested and fixed with ice cold 70% ethanol at 4 °C for 24 h. After the washes, cells were stained with PI staining solution (20 mg/mL PI in 0.3 mL of PBS containing 200–400 unit of RNase A) followed by incubation at 37 °C for 30 min in the dark. The cells were analyzed by flow cytometry (Accuri C6, Becton Dickenson, Bergen, NJ, USA) to determine the proportion of cells within the cycle.

4. Conclusions

In conclusion, we describe the synthesis and examination of a new series of N-substituted benzimidazole derivatives with a functional chalcone group. On the basis of the MTT assay against A549, MCF-7, HEP-G2 and OVCAR-3 cells, we confirmed that these derivatives exhibit considerable potential as anticancer drugs. In particular, compound 23a ((2E)-1-(1-(3-morpholinopropyl)-1H-benzimidazol-2-yl)-3-phenyl-2-propen-1-one) attained high IC50 values on A549, MCF-7, HEP-G2 and OVCAR-3 cells, and showed in vitro cytotoxicity comparable or superior to that of cisplatin. Among the tested derivatives, 11 compounds (20a, 20c, 20d, 21a–21c, 22c, 22d, 23a–23c) had IC50 values between 10.34 and 14.88 μM, which were lower than 16.04 μM (the IC50 value of cisplatin), for the OVCAR-3 cells. According to the MTT results, the nitrogen-containing 5- or 6-membered ring in the N-substituted benzimidazole derivatives can lead to enhanced cytotoxic effects on MCF-7 and OVCAR-3 cells, which might serve as new templates in the synthesis and development of potent therapeutics.
Additionally, the flow cytometry results indicate OVCAR-3 cells treated with compound 20a and 21a were 55% and 63% in the G2/M phase, respectively (Table 2). The activities of compounds 20a and 21a may be related to their abilities to control cells entering apoptosis from the S or G2/M phase of the cell cycle. A similar mechanism was also found in thyroid carcinoma, BHT-101, cells after treatment with 50 μM curcumin [57]. Moreover, compound 23a had low IC50 concentration in OVCR-3 cells, but it did not show any significant change in flow cytometry results. Cytotoxicity of compound 23a may have occurred through cell necrosis.
To further improve the efficacy and specificity for cancer cell death, we conclude that the novel series of N-substituted benzimidazole derivatives can serve as prototype molecules for further development of a new class of anti-cancer agents.

Supplementary Materials

The supporting information are available online.

Author Contributions

H.-C.Y., K.-P.W., C.-Y.J., K.M. performed the synthesis and structure elucidation. L.-Y.C., L.-H.H., C.-H.L. contributed to evaluation of bioactivity. H.-J.C. and H.-M.H. prepared the manuscript and supervised whole research project.

Funding

We thank the National Tsing Hua University and Ministry of Science and Technology of the Republic of China for financial support, also thanks the Laboratory Animal Core Facility which is funded by Agriculture Biotechnology Research Center (ABRC) at Academia Sinica for technical support in cytotoxic, cell morphology and flow cytometry experiments. The authors also thank Ms. Miranda Loney for manuscript editing. This work was supported by the Ministry of Science and Technology (MOST 104-2119-M-007-017)(MOST 106-2113-M -018-008)(MOST 107-2113-M-018-008)(MOST 108-2113-M-018-009).

Acknowledgments

The authors thank to Miranda Jane Loney (ABRC, Academia Sinica, Taiwan) for her attentive editing of this manuscript.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Chimento, A.; Sala, M.; Gomez-Monterrey, I.M.; Musella, S.; Bertamino, A.; Caruso, A.; Sinicropi, M.S.; Sirianni, R.; Puoci, F.; Parisi, O.I.; et al. Biological activity of 3-chloro-azetidin-2-one derivatives having interesting antiproliferative activity on human breast cancer cell lines. Bioorg. Med. Chem. Lett. 2013, 23, 6401–6405. [Google Scholar] [CrossRef]
  2. Rui, M.; Rossi, D.; Marra, A.; Paolillo, M.; Schinelli, S.; Curti, D.; Tesei, A.; Cortesi, M.; Zamagni, A.; Laurini, E.; et al. Synthesis and biological evaluation of new aryl-alkyl(alkenyl)-4-benzylpiperidines, novel Sigma Receptor (SR) modulators, as potential anticancer-agents. Eur. J. Med. Chem. 2016, 124, 649–665. [Google Scholar] [CrossRef] [Green Version]
  3. Jukić, M.; Rastija, V.; Opačak-Bernardi, T.; Stolić, I.; Krstulović, L.; Bajić, M.; Glavaš-Obrovac, L. Antitumor activity of 3,4-ethylenedioxythiophene derivatives and quantitative structure-activity relationship analysis. J. Mol. Struct. 2017, 1133, 66–73. [Google Scholar] [CrossRef]
  4. Townsend, L.B.; Wise, D.S. The synthesis and chemistry of certain anthelmintic benzimidazoles. Parasitol. Today 1990, 6, 107–112. [Google Scholar] [CrossRef]
  5. Woolley, D.W. Some biological effects produced by benzimidazole and their reversal by purines. J. Biol. Chem. 1944, 152, 225–232. [Google Scholar]
  6. Fang, Y.; Zhou, H.; Gu, Q.; Xu, J. Synthesis and evaluation of tetrahydroisoquinoline-benzimidazole hybrids as multifunctional agents for the treatment of Alzheimer’s disease. Eur. J. Med. Chem. 2019, 167, 133–145. [Google Scholar] [CrossRef]
  7. Shimomura, I.; Yokoi, A.; Kohama, I.; Kumazaki, M.; Tada, Y.; Tatsumi, K.; Ochiya, T.; Yamamoto, Y. Drug library screen reveals benzimidazole derivatives as selective cytotoxic agents for KRAS-mutant lung cancer. Cancer Lett. 2019, 451, 11–22. [Google Scholar] [CrossRef]
  8. Tuncbilek, M.; Kiper, T.; Altanlar, N. Synthesis and in vitro antimicrobial activity of some novel substituted benzimidazole derivatives having potent activity against MRSA. Eur. J. Med. Chem. 2009, 44, 1024–1033. [Google Scholar] [CrossRef]
  9. Shingalapur, R.V.; Hosamani, K.M.; Keri, R.S. Synthesis and evaluation of in vitro anti-microbial and anti-tubercular activity of 2-styryl benzimidazoles. Eur. J. Med. Chem. 2009, 44, 4244–4248. [Google Scholar] [CrossRef]
  10. Surineni, G.; Gao, Y.; Hussain, M.; Liu, Z.; Lu, Z.; Chhotaray, C.; Islam, M.M.; Hameed, H.M.A.; Zhang, T. Design, synthesis, and in vitro biological evaluation of novel benzimidazole tethered allylidenehydrazinylmethylthiazole derivatives as potent inhibitors of Mycobacterium tuberculosis. Medchemcomm 2019, 10, 49–60. [Google Scholar] [CrossRef]
  11. Zhang, H.Z.; Damu, G.L.; Cai, G.X.; Zhou, C.H. Design, synthesis and antimicrobial evaluation of novel benzimidazole type of Fluconazole analogues and their synergistic effects with Chloromycin, Norfloxacin and Fluconazole. Eur. J. Med. Chem. 2013, 64, 329–344. [Google Scholar] [CrossRef]
  12. Hernandez-Luis, F.; Hernandez-Campos, A.; Castillo, R.; Navarrete-Vazquez, G.; Soria-Arteche, O.; Hernandez-Hernandez, M.; Yepez-Mulia, L. Synthesis and biological activity of 2-(trifluoromethyl)-1H-benzimidazole derivatives against some protozoa and Trichinella spiralis. Eur. J. Med. Chem. 2010, 45, 3135–3141. [Google Scholar] [CrossRef]
  13. Valdez-Padilla, D.; Rodriguez-Morales, S.; Hernandez-Campos, A.; Hernandez-Luis, F.; Yepez-Mulia, L.; Tapia-Contreras, A.; Castillo, R. Synthesis and antiprotozoal activity of novel 1-methylbenzimidazole derivatives. Bioorg. Med. Chem. 2009, 17, 1724–1730. [Google Scholar] [CrossRef]
  14. Rida, S.M.; El-Hawash, S.A.M.; Fahmy, H.T.Y.; Hazzaa, A.A.; El-Meligy, M.M.M. Synthesis of novel benzofuran and related benzimidazole derivatives for evaluation of in vitro anti-HIV-1, anticancer and antimicrobial activities. Arch. Pharmacal Res. 2006, 29, 826–833. [Google Scholar] [CrossRef]
  15. Luo, Y.; Yao, J.P.; Yang, L.; Feng, C.L.; Tang, W.; Wang, G.F.; Zuo, J.P.; Lu, W. Design and synthesis of novel benzimidazole derivatives as inhibitors of hepatitis B virus. Bioorg. Med. Chem. 2010, 18, 5048–5055. [Google Scholar] [CrossRef]
  16. Singla, P.; Luxami, V.; Paul, K. Benzimidazole-biologically attractive scaffold for protein kinase inhibitors. Rsc Adv. 2014, 4, 12422–12440. [Google Scholar] [CrossRef]
  17. Achar, K.C.; Hosamani, K.M.; Seetharamareddy, H.R. In-vivo analgesic and anti-inflammatory activities of newly synthesized benzimidazole derivatives. Eur. J. Med. Chem. 2010, 45, 2048–2054. [Google Scholar] [CrossRef]
  18. Refaat, H.M. Synthesis and anticancer activity of some novel 2-substituted benzimidazole derivatives. Eur. J. Med. Chem. 2010, 45, 2949–2956. [Google Scholar] [CrossRef]
  19. Azam, M.; Khan, A.A.; Al-Resayes, S.I.; Islam, M.S.; Saxena, A.K.; Dwivedi, S.; Musarrat, J.; Trzesowska-Kruszynska, A.; Kruszynski, R. Synthesis and characterization of 2-substituted benzimidazoles and their evaluation as anticancer agent. Spectrochim. Acta Part A 2015, 142, 286–291. [Google Scholar] [CrossRef]
  20. Pourgholami, M.H.; Woon, L.; Almajd, R.; Akhter, J.; Bowery, P.; Morris, D.L. In vitro and in vivo suppression of growth of hepatocellular carcinoma cells by albendazole. Cancer Lett. 2001, 165, 43–49. [Google Scholar] [CrossRef]
  21. Di Carlo, G.; Mascolo, N.; Izzo, A.A.; Capasso, F. Flavonoids: Old and new aspects of a class of natural therapeutic drugs. Life Sci. 1999, 65, 337–353. [Google Scholar] [CrossRef]
  22. Nowakowska, Z. A review of anti-infective and anti-inflammatory chalcones. Eur. J. Med. Chem 2007, 42, 125–137. [Google Scholar] [CrossRef]
  23. Pettit, G.R.; Rhodes, M.R.; Herald, D.L.; Hamel, E.; Schmidt, J.M.; Pettitt, R.K. Antineoplastic agents. 445. Synthesis and evaluation of structural modifications of (Z)- and (E)-combretastatin A-4. J. Med. Chem. 2005, 48, 4087–4099. [Google Scholar] [CrossRef]
  24. Sivakumar, P.M.; Ganesan, S.; Veluchamy, P.; Doble, M. Novel chalcones and 1,3,5-triphenyl-2-pyrazoline derivatives as antibacterial agents. Chem. Biol. Drug Des. 2010, 76, 407–411. [Google Scholar] [CrossRef]
  25. Agarwal, A.; Srivastava, K.; Puri, S.K.; Chauhan, P.M.S. Synthesis of substituted indole derivatives as a new class of antimalarial agents. Bioorg. Med. Chem. Lett. 2005, 15, 3133–3136. [Google Scholar] [CrossRef]
  26. Suwito, H.; Jumina; Mustofa; Pudjiastuti, P.; Fanani, M.Z.; Kimata-Ariga, Y.; Katahira, R.; Kawakami, T.; Fujiwara, T.; Hase, T.; et al. Design and synthesis of chalcone derivatives as inhibitors of the ferredoxin—ferredoxin-NADP(+) reductase interaction of plasmodium falciparum: Pursuing new antimalarial agents. Molecules 2014, 19, 21473–21488. [Google Scholar]
  27. Boeck, P.; Leal, P.C.; Yunes, R.A.; Cechinel, V.; Lopez, S.; Sortino, M.; Escalante, A.; Furlan, R.L.E.; Zacchino, S. Antifungal activity and studies on mode of action of novel xanthoxyline-derived chalcones. Arch. Pharm. 2005, 338, 87–95. [Google Scholar] [CrossRef]
  28. Cheenpracha, S.; Karalai, C.; Ponglimanont, C.; Subhadhirasakul, S.; Tewtrakul, S. Anti-HIV-1 protease activity of compounds from Boesenbergia pandurata. Bioorg. Med. Chem. 2006, 14, 1710–1714. [Google Scholar] [CrossRef]
  29. Mateeva, N.; Gangapuram, M.; Mazzio, E.; Eyunni, S.; Soliman, K.F.A.; Redda, K.K. Biological evaluation of synthetic chalcone and flavone derivatives as anti-inflammatory agents. Med. Chem. Res. 2015, 24, 1672–1680. [Google Scholar] [CrossRef]
  30. Kamal, A.; Prabhakar, S.; Ramaiah, M.J.; Reddy, P.V.; Reddy, C.R.; Mallareddy, A.; Shankaraiah, N.; Reddy, T.L.N.; Pushpavalli, S.N.C.V.L.; Pal-Bhadra, M. Synthesis and anticancer activity of chalcone-pyrrolobenzodiazepine conjugates linked via 1,2,3-triazole ring side-armed with alkane spacers. Eur. J. Med. Chem. 2011, 46, 3820–3831. [Google Scholar] [CrossRef]
  31. Kim, T.H.; Seo, W.D.; Ryu, H.W.; Seo, H.R.; Jin, Y.B.; Lee, M.; Ji, Y.H.; Park, K.H.; Lee, Y.S. Anti-tumor effects by a synthetic chalcone compound is mediated by c-Myc-mediated reactive oxygen species production. Chem.-Biol. Interact. 2010, 188, 111–118. [Google Scholar] [CrossRef]
  32. Modzelewska, A.; Pettit, C.; Achanta, G.; Davidson, N.E.; Huang, P.; Khan, S.R. Anticancer activities of novel chalcone and bis-chalcone derivatives. Bioorg. Med. Chem. 2006, 14, 3491–3495. [Google Scholar] [CrossRef]
  33. Quintin, J.; Desrivot, J.; Thoret, S.; le Menez, P.; Cresteil, T.; Lewin, G. Synthesis and biological evaluation of a series of tangeretin-derived chalcones. Bioorg. Med. Chem. Lett. 2009, 19, 167–169. [Google Scholar] [CrossRef]
  34. He, S.W.; Hong, Q.M.; Lai, Z.; Yang, D.X.; Ting, P.C.; Kuethe, J.T.; Cernak, T.A.; Dykstra, K.D.; Sperbeck, D.M.; Wu, Z.C.; et al. Discovery of a potent and selective DGAT1 inhibitor with a piperidinyl-oxy-cyclohexanecarboxylic acid moiety. ACS Med. Chem. Lett. 2014, 5, 1082–1087. [Google Scholar] [CrossRef]
  35. Shaharyar, M.; Abdullah, M.M.; Bakht, M.A.; Majeed, J. Pyrazoline bearing benzimidazoles: Search for anticancer agent. Eur. J. Med. Chem. 2010, 45, 114–119. [Google Scholar] [CrossRef]
  36. Solomon, V.R.; Hu, C.K.; Lee, H. Hybrid pharmacophore design and synthesis of isatin-benzothiazole analogs for their anti-breast cancer activity. Bioorg. Med. Chem. 2009, 17, 7585–7592. [Google Scholar] [CrossRef]
  37. Wan, M.S.; Xu, L.Y.; Hua, L.; Li, A.L.; Li, S.Q.; Lu, W.J.; Pang, Y.; Cao, C.B.; Liu, X.G.; Jiao, P. Synthesis and evaluation of novel isoxazolyl chalcones as potential anticancer agents. Bioorg. Chem. 2014, 54, 38–43. [Google Scholar] [CrossRef]
  38. Ouattara, M.; Sissouma, D.; Kone, M.W.; Menan, H.E.; Toure, S.A.; Ouattara, L. Synthesis and anthelmintic activity of some hybrid Benzimidazolyl-chalcone derivatives. Trop. J. Pharm. Res. 2011, 10, 767–775. [Google Scholar] [CrossRef]
  39. Reddy, V.M.; Reddy, K.R. Synthesis and biological evaluation of some novel-3-(5-substituted benzimidazol-2-yl)-5-arylisoxazolines. Chin. Chem. Lett. 2010, 21, 1145–1148. [Google Scholar] [CrossRef]
  40. Mathew, B.; Suresh, J.; Vinod, D. Antitumour activity of 5-[(2E)-1-(1H-benzimidazol-2-yl)-3-substituted phenylprop-2-en-1-ylidene] pyrimidine-2,4,6(1H,3H,5H)-triones against Dalton’s ascitic lymphoma in mice. Med. Chem. Res. 2013, 22, 3911–3917. [Google Scholar] [CrossRef]
  41. Woo, H.B.; Eom, Y.W.; Park, K.S.; Ham, J.; Ahn, C.M.; Lee, S. Synthesis of substituted benzimidazolyl curcumin mimics and their anticancer activity. Bioorg. Med. Chem. Lett. 2012, 22, 933–936. [Google Scholar] [CrossRef]
  42. Hayes, M.E.; Wallace, G.A.; Grongsaard, P.; Bischoff, A.; George, D.M.; Miao, W.Y.; McPherson, M.J.; Stoffel, R.H.; Green, D.W.; Roth, G.P. Discovery of small molecule benzimidazole antagonists of the chemokine receptor CXCR3. Bioorg. Med. Chem. Lett. 2008, 18, 1573–1576. [Google Scholar] [CrossRef]
  43. Mehboob, S.; Song, J.H.; Hevener, K.E.; Su, P.C.; Boci, T.; Brubaker, L.; Truong, L.; Mistry, T.; Deng, J.P.; Cook, J.L.; et al. Structural and biological evaluation of a novel series of benzimidazole inhibitors of Francisella tularensis enoyl-ACP reductase (FabI). Bioorg. Med. Chem. Lett. 2015, 25, 1292–1296. [Google Scholar] [CrossRef] [Green Version]
  44. Sharma, A.; Luxami, V.; Paul, K. Synthesis, single crystal and antitumor activities of benzimidazole-quinazoline hybrids. Bioorg. Med. Chem. Lett. 2013, 23, 3288–3294. [Google Scholar] [CrossRef]
  45. Hwu, J.R.; Hsu, M.H.; Huang, R.C.C. New nordihydroguaiaretic acid derivatives as anti-HIV agents. Bioorg. Med. Chem. Lett. 2008, 18, 1884–1888. [Google Scholar] [CrossRef]
  46. Orjales, A.; Alonso-Cires, L.; Lopez-Tudanca, P.; Tapia, I.; Mosquera, R.; Labeaga, L. Benzimidazole-2-carboxylic acid amides and esters: A new structural class of 5-HT(3) ligands. Eur. J. Med. Chem. 1999, 34, 415–422. [Google Scholar] [CrossRef]
  47. Ganot, N.; Meker, S.; Reytman, L.; Tzubery, A.; Tshuva, E.Y. Anticancer metal complexes: Synthesis and cytotoxicity evaluation by the MTT assay. J. Vis. Exp. 2013, 81, 507–567. [Google Scholar] [CrossRef]
  48. Grigalius, I.; Petrikaite, V. Relationship between antioxidant and anticancer activity of trihydroxyflavones. Molecules 2017, 22, 2169. [Google Scholar] [CrossRef]
  49. Mosmann, T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods 1983, 65, 55–63. [Google Scholar] [CrossRef]
  50. Li, F.Y.; Wang, X.; Duan, W.G.; Lin, G.S. Synthesis and in vitro anticancer activity of novel dehydroabietic acid-based acylhydrazones. Molecules 2017, 22, 1087. [Google Scholar] [CrossRef]
  51. Sliwka, L.; Wiktorska, K.; Suchocki, P.; Milczarek, M.; Mielczarek, S.; Lubelska, K.; Cierpial, T.; Lyzwa, P.; Kielbasinski, P.; Jaromin, A.; et al. The comparison of MTT and CVS assays for the assessment of anticancer agent interactions. PLoS ONE 2016, 11. [Google Scholar] [CrossRef]
  52. Murakami, T. Absorption sites of orally administered drugs in the small intestine. Expert Opin. Drug Discov. 2017, 12, 1219–1232. [Google Scholar] [CrossRef]
  53. Tetko, I.V.; Bruneau, P. Application of ALOGPS to predict 1-octanol/water distribution coefficients, logP, and logD, of AstraZeneca in-house database. J. Pharm. Sci. 2004, 93, 3103–3110. [Google Scholar] [CrossRef]
  54. Tetko, I.V.; Tanchuk, V.Y. Application of associative neural networks for prediction of lipophilicity in ALOGPS 2.1 program. J. Chem. Inf. Comput. Sci. 2002, 42, 1136–1145. [Google Scholar] [CrossRef]
  55. Tetko, I.V.; Tanchuk, V.Y.; Kasheva, T.N.; Villa, A.E. Internet software for the calculation of the lipophilicity and aqueous solubility of chemical compounds. J. Chem. Inf. Comput. Sci. 2001, 41, 246–252. [Google Scholar] [CrossRef]
  56. Desai, N.C.; Pandya, D.D.; Joshi, V.V.; Rajpara, K.M.; Vaghani, H.V.; Satodiya, H.M. Synthesis, characterization and antimicrobial screening of hybrid molecules containing benzimidazole-pyrazole and pyridine nucleus. Med. Chem. Res. 2012, 21, 4463–4472. [Google Scholar] [CrossRef]
  57. Schwertheim, S.; Wein, F.; Lennartz, K.; Worm, K.; Schmid, K.W.; Sheu-Grabellus, S.Y. Curcumin induces G2/M arrest, apoptosis, NF-kappaB inhibition, and expression of differentiation genes in thyroid carcinoma cells. J. Cancer Res. Clin. Oncol. 2017, 143, 1143–1154. [Google Scholar] [CrossRef]
Sample Availability: Samples of the compounds are available from the authors.
Molecules 24 03259 g001 550
Figure 1. Benzimidazole analogs with various biological activities.
Figure 1. Benzimidazole analogs with various biological activities.
Molecules 24 03259 g001
Molecules 24 03259 g002 550
Figure 2. Structures of chalcone (7), combretastatin (8), and bis-chalcones (9).
Figure 2. Structures of chalcone (7), combretastatin (8), and bis-chalcones (9).
Molecules 24 03259 g002
Molecules 24 03259 g003 550
Figure 3. Reported and proposed anticancer benzimidazole derivatives.
Figure 3. Reported and proposed anticancer benzimidazole derivatives.
Molecules 24 03259 g003
Molecules 24 03259 sch001 550
Scheme 1. Reagents and conditions: (i) lactic acid (1.1 equiv), 4N HCl, reflux, 6 h; (ii) permanganate (2.5 equiv), solid aluminium oxide, no solvent, room temperature, 10 min; (iii) benzaldehyde, 40% KOH, ethanol, room temperature, 10 min; (iv) potassium carbonate, acetonitrile, reflux, overnight.
Scheme 1. Reagents and conditions: (i) lactic acid (1.1 equiv), 4N HCl, reflux, 6 h; (ii) permanganate (2.5 equiv), solid aluminium oxide, no solvent, room temperature, 10 min; (iii) benzaldehyde, 40% KOH, ethanol, room temperature, 10 min; (iv) potassium carbonate, acetonitrile, reflux, overnight.
Molecules 24 03259 sch001
Table
Table 1. Calculation of lipophilicity and water solubility of the benzimidazole derivatives library and the relationship between cytotoxicity against MCF-7 and OVCAR-3 cell line. (logP and logS were calculated from http://www.vcclab.org/lab/alogps/). IC50 values given are mean ± SD of two separate experiments in duplicate.
Table 1. Calculation of lipophilicity and water solubility of the benzimidazole derivatives library and the relationship between cytotoxicity against MCF-7 and OVCAR-3 cell line. (logP and logS were calculated from http://www.vcclab.org/lab/alogps/). IC50 values given are mean ± SD of two separate experiments in duplicate.
CompoundLipophilicity and Water SolubilityCancer Cells (IC50 μM)
M.W.clogPclogSS(cal) (mmol/L)A549MCF-7HEP-G2OVCAR-3
18a248.293.51−4.3810.35119.3 ± 29.913.49 ± 0.1624.2 ± 0.3216.91 ± 0.37
18b262.313.80−4.675.6119.17 ± 0.4318.09 ± 0.2859.13 ± 0.9224.7 ± 1.69
18c278.313.41−4.567.6717.41 ± 0.1616.04 ± 0.24140.85 ± 0.8834.44 ± 1.55
18d282.734.01−5.072.4135.89 ± 0.8432.55 ± 3.2636.54 ± 1.3536.48 ± 1.36
19a288.354.19−4.587.5812.47 ± 0.1812.12 ± 0.1015.44 ± 0.2516.09 ± 0.39
19b302.384.30−4.903.8141.05 ± 1.6153.54 ± 1.12117.28 ± 2.4259.01 ± 8.91
19c318.384.20−4.814.93>314254.9 ± 13.6>314299.52 ± 9.27
19d322.794.57−5.042.9415.79 ± 0.4913.42 ± 0.2417.6 ± 0.2516.13 ± 0.32
20a345.454.20−4.618.4810.3 ± 0.139.65 ± 0.0610.16 ± 0.0810.5 ± 0.10
20b359.474.55−4.904.5354.12 ± 1.2053.19 ± 0.7764.91 ± 0.2428.71 ± 1.44
20c375.474.28−4.805.9556.21 ± 0.9656.09 ± 0.1436.61 ± 1.8911.4 ± 0.24
20d379.894.71−5.033.5519.53 ± 0.7114.73±0.0915.49±0.1614.04 ± 0.29
21a359.474.59−4.874.8510.73 ± 0.589.73 ± 0.1610.33 ± 0.0610.34 ± 0.19
21b373.54.90−5.102.9711.64 ± 0.2511.14 ± 0.0732.16 ± 1.8312.55 ± 0.12
21c389.504.70−5.073.3222.36 ± 0.5421.12 ± 0.5358.74 ± 0.7513.29 ± 0.47
21d393.925.09−5.272.1250.45 ± 0.8254.41 ± 0.7256.45 ± 0.8633.13 ± 0.14
22a361.453.37−4.3316.9114.59 ± 0.4010.38 ± 0.0836.13 ± 0.7522.44±0.47
22b375.473.74−4.658.4110.76 ± 0.2910.15 ± 0.0642.05 ± 0.9116.32 ± 0.45
22c391.473.35−4.5211.8210.27 ± 0.1511.12 ± 0.2050.24 ± 0.8814.88 ± 0.67
22d395.893.92−4.835.8624.06 ± 0.0822.93 ± 0.4921.38 ± 0.6814.22 ± 0.33
23a375.473.82−4.678.039.73 ± 0.078.91 ± 0.0710.93 ± 0.1010.76 ± 0.12
23b389.54.12−4.934.5811.79 ± 0.2711.34 ± 0.1747.88 ± 0.7613.76 ± 0.27
23c405.53.84−4.836.0016.92 ± 0.6111.93 ± 0.1432.92 ± 0.3813.4 ± 0.33
23d409.914.32−5.103.2681.48 ± 1.4035.69 ± 0.4795.7 ± 2.4442.24 ± 2.43
DOX 0.46 ± 0.010.42 ± 0.010.72 ± 0.013.95 ± 0.09
Cisplatin 7.31 ± 0.4411.7 ± 0.123.97 ± 0.0416.04 ± 0.74
Table
Table 2. Cell cycle distribution of ovarian carcinoma OVCR-3 with different treatments.
Table 2. Cell cycle distribution of ovarian carcinoma OVCR-3 with different treatments.
TreatmentG1 (%)S (%)G2/M (%)IC50 (μM)
Control (PBS)55.268.0036.74−
20a37.038.0054.9610.50
21a29.378.0062.6310.34
22a62.588.0029.4222.44
23a60.248.0031.7610.76
Percentage of cells was from 10,000 counts in G1, S and G2/M phases of cell cycle according to FACS-analysis.

Share and Cite

MDPI and ACS Style

Hsieh, C.-Y.; Ko, P.-W.; Chang, Y.-J.; Kapoor, M.; Liang, Y.-C.; Chu, H.-L.; Lin, H.-H.; Horng, J.-C.; Hsu, M.-H. Design and Synthesis of Benzimidazole-Chalcone Derivatives as Potential Anticancer Agents. Molecules 2019, 24, 3259. https://doi.org/10.3390/molecules24183259

AMA Style

Hsieh C-Y, Ko P-W, Chang Y-J, Kapoor M, Liang Y-C, Chu H-L, Lin H-H, Horng J-C, Hsu M-H. Design and Synthesis of Benzimidazole-Chalcone Derivatives as Potential Anticancer Agents. Molecules. 2019; 24(18):3259. https://doi.org/10.3390/molecules24183259

Chicago/Turabian Style

Hsieh, Cheng-Ying, Pi-Wen Ko, Yu-Jui Chang, Mohit Kapoor, Yu-Chuan Liang, Hsueh-Liang Chu, Hui-Hsien Lin, Jia-Cherng Horng, and Ming-Hua Hsu. 2019. "Design and Synthesis of Benzimidazole-Chalcone Derivatives as Potential Anticancer Agents" Molecules 24, no. 18: 3259. https://doi.org/10.3390/molecules24183259

APA Style

Hsieh, C. -Y., Ko, P. -W., Chang, Y. -J., Kapoor, M., Liang, Y. -C., Chu, H. -L., Lin, H. -H., Horng, J. -C., & Hsu, M. -H. (2019). Design and Synthesis of Benzimidazole-Chalcone Derivatives as Potential Anticancer Agents. Molecules, 24(18), 3259. https://doi.org/10.3390/molecules24183259

Article Metrics

Back to TopTop