Characterization of Potential Protein Biomarkers for Major Depressive Disorder Using Matrix-Assisted Laser Desorption Ionization/Time-of-Flight Mass Spectrometry
Abstract
:1. Introduction
2. Results and Discussion
2.1. Direct Analysis of Plasma Samples by MALDI-TOF Mass Spectrometry
2.2. Optimization for Acid Hydrolysis of Plasma Samples Followed by MALDI-TOF Analysis
2.3. Optimized MALDI-TOF Analysis for Plasma Samples
2.4. Principal Component Analysis
2.5. Hierarchical Clustering Analysis
2.6. Receiver Operating Characteristic Curve Analysis
2.7. Biomarker Identification and Clinical Implications
3. Materials and Methods
3.1. Plasma Samples Collection and Storage
3.2. Chemicals and Materials
3.3. Acid Hydrolysis
3.4. Mass Spectrometric Analyses
3.5. Multivariate Statistical Analyses
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Healthy | Schizophrenia | Major Depressive Disorder | ||||||
---|---|---|---|---|---|---|---|---|
Code | Gender | Age | Code | Gender | Age | Code | Gender | Age |
HCBC6-1 | Male | 23 | BE34-1 | Male | 29 | CNN48-1 | Male | 42 |
HCBC9-1 | 24 | BE38-1 | 42 | CNN49-1 | 26 | |||
HCBC39-1 | 40 | BE41-1 | 34 | CNC56-1 | 20 | |||
HCBC53-1 | 37 | BE81-1 | 37 | CNC74-1 | 47 | |||
HCBC93-1 | 40 | BE125-1 | 21 | CNC77-1 | 37 | |||
HCBC106-1 | 21 | BE202-1 | 47 | CNC78-1 | 32 | |||
HCBC129-1 | 35 | BE207-1 | 39 | CNC90-1 | 22 | |||
HCBC139-1 | 48 | DRM104 | 23 | CNC95-1 | 42 | |||
HCBC152-1 | 29 | DRM109 | 33 | CNC106-1 | 29 | |||
HCBC153-1 | 32 | RNMak-1 | 25 | CNC107-1 | 35 | |||
n = 10 | 32.9 (avg.) | n = 10 | 33.0 (avg.) | n = 10 | 33.2 (avg.) | |||
HCBC3 | Female | 22 | BE66-1 | Female | 29 | CNN38-1 | Female | 26 |
HCBC15-1 | 26 | BE71-1 | 32 | CNN43-1 | 25 | |||
HCBC20-1 | 19 | BE201-2 | 26 | CNN50-2 | 28 | |||
HCBC31-1 | 27 | BED008-1 | 19 | CNC52-1 | 41 | |||
HCBC41-1 | 34 | BED009-1 | 26 | CNC60-1 | 37 | |||
HCBC110 | 36 | DRM102 | 21 | CNC63-1 | 32 | |||
HCBC131-1 | 28 | DRM103 | 27 | CNC73-1 | 23 | |||
HCBC135-1 | 26 | DRM110 | 36 | CNC79-1 | 26 | |||
HCBC141-1 | 32 | RNMg | 34 | CNC88-2 | 34 | |||
HCBC151-1 | 41 | RNNt-1 | 40 | CNC89-1 | 19 | |||
n = 10 | 29.1 (avg.) | n = 10 | 29.0 (avg.) | n = 10 | 29.1 (avg.) | |||
20 (total) | 31.0 (F + M) | 20 (total) | 31.0 (F + M) | 20 (total) | 31.15 (F + M) |
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Lin, C.-H.; Su, H.; Hung, C.-C.; Lane, H.-Y.; Shiea, J. Characterization of Potential Protein Biomarkers for Major Depressive Disorder Using Matrix-Assisted Laser Desorption Ionization/Time-of-Flight Mass Spectrometry. Molecules 2021, 26, 4457. https://doi.org/10.3390/molecules26154457
Lin C-H, Su H, Hung C-C, Lane H-Y, Shiea J. Characterization of Potential Protein Biomarkers for Major Depressive Disorder Using Matrix-Assisted Laser Desorption Ionization/Time-of-Flight Mass Spectrometry. Molecules. 2021; 26(15):4457. https://doi.org/10.3390/molecules26154457
Chicago/Turabian StyleLin, Chieh-Hsin, Hung Su, Chung-Chieh Hung, Hsien-Yuan Lane, and Jentaie Shiea. 2021. "Characterization of Potential Protein Biomarkers for Major Depressive Disorder Using Matrix-Assisted Laser Desorption Ionization/Time-of-Flight Mass Spectrometry" Molecules 26, no. 15: 4457. https://doi.org/10.3390/molecules26154457
APA StyleLin, C. -H., Su, H., Hung, C. -C., Lane, H. -Y., & Shiea, J. (2021). Characterization of Potential Protein Biomarkers for Major Depressive Disorder Using Matrix-Assisted Laser Desorption Ionization/Time-of-Flight Mass Spectrometry. Molecules, 26(15), 4457. https://doi.org/10.3390/molecules26154457