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Article

Antiparasitic Potential of Chromatographic Fractions of Nephrolepis biserrata and Liquid Chromatography-Quadrupole Time-of-Flight-Mass Spectrometry Analysis

by
Muhammad Dawood Shah
1,
Kazuki Tani
2,
Yoong Soon Yong
3,
Fui Fui Ching
1,
Sitti Raehanah Muhamad Shaleh
1,
Charles S. Vairappan
2 and
Balu Alagar Venmathi Maran
1,*
1
Borneo Marine Research Institute, Universiti Malaysia Sabah, Kota Kinabalu, Jalan UMS 88450, Sabah, Malaysia
2
Laboratory of Natural Products Chemistry, Institute for Tropical Biology and Conservation, Universiti Malaysia Sabah, Kota Kinabalu, Jalan UMS 88450, Sabah, Malaysia
3
Laboratory Centre, Xiamen University Malaysia, Jalan Sunsuria, Bandar Sunsuria, Sepang 43900, Selangor, Malaysia
*
Author to whom correspondence should be addressed.
Molecules 2021, 26(2), 499; https://doi.org/10.3390/molecules26020499
Submission received: 30 November 2020 / Revised: 31 December 2020 / Accepted: 31 December 2020 / Published: 19 January 2021
(This article belongs to the Special Issue Natural Products and Their Semi-synthetic Derivatives against Bacteria, Fungi and Parasites)
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Abstract

:
Marine aquaculture development is recently impeded by parasitic leech Zeylanicobdella arugamensis (Hirudinea, Piscicolidae) in Sabah, Malaysia. The parasitic leech infests a variety of cultured fishes in aquaculture facilities. In this study, we evaluated the antiparasitic activity of the chromatographic fractions of the medicinal plant Nephrolepis biserrata methanol extract against Z. arugamensis and highlighted the potential metabolites responsible for the antiparasitic properties through liquid chromatography (LC)–quadrupole time-of-flight (QTOF)–mass spectrometry (MS) analysis. Out of seven fractions obtained through flash column chromatography techniques, three fractions demonstrated antiparasitic properties. Significant parasitic mortality was indicated by fraction 3 at a concentration of 2.50 mg/mL, all the leeches were killed in a time limit of 1.92 ± 0.59 min. followed by fraction 4 (14 mg/mL) in 34.57 ± 3.39 and fraction 5 (15.3 mg/mL) in 36.82 ± 4.53 min. LC-QTOF-MS analysis indicated the presence of secondary metabolites including phytosphingosine (6), pyrethrosin (1), haplophytine (9), ivalin (2), warburganal (3), isodomedin (4) and pheophorbide a (16), representing sphingoid, alkaloid, terpenoid, phenolic and flavonoid groups. Thus, our study indicated that the chromatographic fractions of N. biserrata demonstrated significant antiparasitic activity against the marine parasitic leeches due to the presence of potent antiparasitic bioactive compounds.

Graphical Abstract

1. Introduction

The high demand for the fish product and decrement of ocean fisheries due to overfishing and deterioration of coastal environments which have triggered the rapid development of aquaculture facilities in Malaysia and other parts of the world, lead to the expansion of brackish-water and marine aquaculture [1,2]. Accordingly, the reputation of parasites as disease-causing agents in aquaculture has become evident [3,4]. Fish parasites are not only responsible for commercial losses in aquaculture and fisheries industries but also cause socio-economic implications both in developing and developed countries [5,6]. Different types of parasites including leeches (Annelida), monogeneans (Platyhelminthes), caligids (Crustacea), etc., are affecting various species of fishes [7,8,9,10].
Among the marine parasitic leeches, Zeylanicobdella arugamensis de Silva, 1963 (Hirudinea, Piscicolidae) is commonly found in Malaysia, Indonesia, Brunei, Philippines, Japan and other South-East Asian countries [3,4,7,11,12,13]. The leech is characterized by having an asymmetrical flattened cylindrical body with anterior and posterior suckers and has been considered to be the most harmful ectoparasite affecting a large variety of species of fish [3,7]. The mortality of the host fish typically occurs within three days following infection due to secondary pathogenic bacterial invasion [3].
Due to the lack of registered biocontrol agents, farmers use toxic chemicals including formalin for the removal of parasites in aquaculture industries [14]. The chemicals are extremely harmful to fish, humans and the environment [15]. Thus, it is very important to develop a natural control agent to minimize the application of toxic chemicals. A plant can be used as a biocontrol agent due to the presence of different antiparasitic phytochemicals. Nephrolepis biserrata (Nephrolepidaceae) is a tropical fern known as “Paku larat” in Malay [16,17]. The plant is a good source of antioxidant compounds with hepatoprotective potential [18]. The antimicrobial activity of the plant has been reported [19], however, the antiparasitic potential of the plant has not yet been reported. Thus, in the current study, we analyzed the antiparasitic potential of the chromatographic fractions of N. biserrata methanol extract against the marine parasitic leech and profiled the phytochemical composition via LC-QTOF-MS analysis.

2. Results

2.1. Antiparasitic Activity of the Chromatographic Fractions of N. biserrata Methanol Extract

The sample plant was extracted with methanol and further chromatographic fractions of the methanol extract were obtained by flash column chromatography techniques. Various concentrations of the chromatographic fractions of N. biserrata methanol extract were prepared and the leeches were exposed (Figure 1). The death time and percentage of the seawater-, formalin- and fractions-treated groups are indicated in Table 1 (Table S1). No mortality was noticed in the negative control group (Figure 1a) while 100 per cent mortality was recorded in the positive control group treated with 0.25% v/v of formalin solution (Figure 1b). In the groups treated with fractions 1, 2, 6 and 7 no mortalities of the leeches were observed while complete mortality was noticed in the groups treated with fractions 3, 4 and 5. In this regard, the group treated with fraction 3 killed all the leeches in a time limit of 1.92 ± 0.59 min at a concentration of 2.50 mg/mL, while fractions 4 and 5 killed all the leeches in 34.57 ± 3.39 (14.0 mg/mL) and 36.82 ± 4.53 min (15.3 mg/mL) (Figure 1c,d). The leeches were considered as dead only when they were not able to move their body even after touching with forceps (Figure 1b–d).

2.2. Behavioural Changes Induced by the Chromatographic Fractions of N. biserrata Methanol Extract

The leeches treated with seawater indicated no behavioral changes. They were attached firmly to the bottom of the plate using their caudal or posterior sucker, demonstrating a normal swimming pattern (Figure 1). The exposure of parasitic leeches to fractions 3, 4 and 5 groups resulted in behavioral changes. The parasitic leeches treated with fraction 3 showed aggressive movements for the first 0.5–1 min. Then, the leeches were not able to attach using their posterior suckers to the bottom of the plate. After 1 min., the leeches became too weak, stopped movement and were dead eventually. Similarly, the leeches exposed to fractions 4 and 5 indicated aggressive swimming for the first 5–10 min. and then their activity decreased slowly. After 25–30 min., the leeches became too weak and showed no movement, even after touched using forceps and finally considered them as died. No behavioral changes were noticed in the groups treated with fractions 1, 2, 6 and 7 of the chromatographic fractions of N. biserrata methanol extract, they were swimming normally and attached firmly to the plate surface.

2.3. Phytochemical Compounds Detected in the Chromatographic Fractions of N. biserrata Methanol Extract

All seven frictions of N. biserrata extract were subjected to LC-QTOF-MS analysis. Among the 566 molecular features extracted via LC-QTOF-MS analysis, a total of 34 secondary metabolites and one chlorophyll breakdown compound were matched successfully. Some of these secondary metabolites were pyrethrosin (1), ivalin (2), warburganal (3), isodomedin (4), 3′-deoxydryopteric acid (5), phytosphingosine (6), 8-oxo-9,11-octadecadiynoic acid (7), 10,16-heptadecadien-8-ynoic acid (8), haplophytine (9), 5,2′,4′-trihydroxy-3,7,8,5′-tetramethoxy flavone (10), 3,5,6,7-tetramethoxy flavone (11), 3,4,5-trimethoxycinnamic acid (12), 3,4-methylenedioxy-2′,4′-dimethoxychalcone (13), 9-tetradecenyl acetate (14), 3,7,17,23-tetrahydroxycholan-24-oic acid (15), and pheophorbide a (16). In overall, we successfully matched six terpenoids, four phenolics, one sphingoid, four fatty acids, two alkaloids, six flavonoids, six aromatics, one aliphatic acetate and four steroids. The retention time, molecular formula and class of the secondary metabolites matched in the fractions of N. biserrata via LC-QTOF-MS analysis are shown in Table 2 along with their chromatograms (Figure 2a–c). While the chemical structures of fraction 3, 4 and 5 metabolites are indicated in Figure 3a–c.

3. Discussion

To minimize the consumption of toxic chemicals against parasites in aquaculture industries, development and application of natural agent is crucial [7,20]. For this purpose, the phytotherapy of fish disinfestation against parasites and diseases is a good alternative due to the presence of various phytochemical compounds [21]. The chromatographic fractions 3, 4 and 5 of the methanol extract of N. biserrata demonstrated significant antiparasitic potential against the marine parasitic leech.
Compared to our study some other plants have also been reported with antiparasitic properties such as the chromatographic fractions of the methanol extract of Dillenia suffruticosa (Dilleneacea) (known as “Simpoh ayer” in Malay) [22]. A fraction of the methanol extract of D. suffruticosa resulted in the total mortality of Z. arugamensis at a concentration of 20 mg/mL in a time limit of 31 min [22]. The active component of Piper nigrum (Piperaceae) (black pepper) has been applied against branchiuran ectoparasite Argulus spp. infesting goldfish Carassius auratus at a concentration ranging from 1 to 9 mg/L. Total mortality (100%) of the parasite has been reported at 9.0 mg/L in 3 h [23]. The methanol extract of Radix Bupleuri chinensis (roots of Bupleuri chinensis, known as “Chai Hu” in Chinese) (Umbelliferae) tested for their in vivo antiparasitic properties against monogenean Dactylogyrus intermedius in goldfish. The parasites were exposed to the extract at a concentration of 10 mg/L and more than 90% of elimination was recorded in 48 h [24]. The methanol extract of Magnolia officinalis (Magnoliaceae) (known as “Houpo” in Chinese) and Sophora alopecuroides (Leguminosae) (known as “Ku dou zi” in Chinese) plants have been reported as having antiparasitic properties [25,26,27]. The methanol extract of M. officinalis and S. alopecuroides at a concentration of 40 mg/L and 120 mg/L, respectively, were applied against the ciliate parasite Ichthyophthirius multifiliis infesting goldfish for 1 h. The extract of the plants reduced the prevalence of parasites by 24.7% and 44.7%, respectively [27]. From the abovementioned different studies, it is evidently proved that the medicinal plants have the potent antiparasitic properties [22,23,24,25,26,27]. Compared to these plants, the chromatographic fractions of the methanol extract of our sample have shown much better activity such as fraction 3 killing all leeches in 1.92 min. In addition to this, the mortality time shown by fraction 3 was almost half compared to formalin (3.90 min) (Table 1). It is interesting to reveal that the fraction 3 of N. biserrata is more effective than formalin in addition to the safe and healthy nature of the plant [7,15,28].
The LCMS analysis indicated the presence of various metabolites with antimicrobial, anti-inflammatory and antiparasitic potential [29,30,31,32,33,34,35]. Though there is a lack of information regarding the antiparasitic potential of these metabolites against marine parasites, their effectiveness against other parasites were widely reported [29,30,31,32,33,34,35]. Some of the important metabolites are phytosphingosine (6), a naturally occurring lipid which acts as antimicrobial (gram-positive bacteria, yeast and moulds) and anti-inflammatory [29]. It also induces mitochondria-mediated apoptosis (cell death) by the fragmentation of chromatin DNA in human T-cell lymphoma Jurkat cells [30]. Pheophorbide a (16), a chlorophyll breakdown product, has been reported to have antiparasitic properties against Leishmania amazonensis, responsible for the disease leishmaniasis [31]. Additionally, the bioactive compound has been reported as having antiviral, antiinflammatory, antioxidant and immunomodulatory properties [32,33,34,35]. Haplophytine (9), an alkaloid, has been reported to have insecticidal properties [36]. These 3 metabolites are detected in fraction 3 of N. biserrata which indicated very strong antiparasitic properties by killing all the leeches in less than 2 min (Table 1). It is suggested that the effectiveness of fraction 3 could be due to the presence of these metabolites. Ivalin (2), a terpenoid, has been reported to have antiparasitic activity against the Trypanosoma brucei rhodesiense and T. cruzi [37]. It also induces apoptosis in human hepatocellular carcinoma SMMC-7721 Cells [38]. Another terpenoid, warburganal (3), along with polygodial bioactive compounds, has been reported with antihelminthic (Caenorhabditis elegans) activity [39]. Warburganal has also been reported with molluscicidal properties [40]. Isodomedin (4), a diterpene, has been reported with antifeedant properties against the larvae of African armyworm, Spodoptera exenzpta [41]. These compounds have been detected in fractions 4 and 5 of N. biserrata (Table 2). Though different types of bioactive compounds were also detected in fractions 1, 2, 6, and 7, as well, no significant inhibition or behavioral changes of Z. arugamensis were observed. These phenomena might be due to the resistance of Z. arugamensis toward these compounds or the presence of low contents of these bioactive compounds.

4. Materials and Methods

4.1. Chemicals

A mixture of hexane and ethyl acetate was used for column preparation and fractionation. Silica gel (SiO2) and normal phase thin layer chromatography plate were obtained from Merck, Germany. Developed TLCs were visualized using 5 per cent molybdophosphoric acid in ethanol was purchased from Nacalai, Japan. Formalin (37 per cent aqueous formaldehyde solution) was bought from Sigma, Leica, Microsystem (Darmstadt, Germany), Methanol (HPLC grade) was purchased from Merck (Darmstadt, Germany). LCMS-grade acetonitrile was obtained from J. T. Baker (Philipsburg, NJ, USA). Polyvinylidene fluoride (PVDF) syringe filters (0.22 µm pore size and 13 mm diameter) were bought from Merck (Darmstadt, Germany). Deionized water was acquired via a Milli-Q system (Merck, Darmstadt, Germany) at a resistivity of >18.2 MΩ·cm. Reference mass solution containing 5.0 mM of purine and 2.5 mM of Hexakis [1H,1H,3H-tetrafluoropropoxy] phosphazine, was procured from Agilent Technologies (Santa Clara, CA, USA). LCMS-grade formic acid (HCOOC) was acquired from Fisher Scientific (Fair Lawn, NJ, USA).

4.2. Plant Collection

Aerial parts of the plants were obtained from Universiti Malaysia Sabah (5.7346° N, 115.9319° E) in Sept 2019 on a sunny day with a minimum temperature of 24 and a maximum temperature of 35 °C. The plant was identified, a voucher specimen was deposited at the Institute for Tropical Biology and Conservation, Universiti Malaysia Sabah, Kota Kinabalu, Malaysia (Figure 4).

4.3. Extraction and Fractionation

The leaves of the plants were cleaned with distilled water and dried by oven at 37 °C. The dried sample was grounded in a heavy-duty grinder and placed in an airtight jar. Methanol (300 mL, HPLC grade) was used for the extraction of the powder (60 g) via the Soxhlet method (50–60 °C for 72 h). The dry extract was recovered using a vacuum rotary evaporator, before lyophilization using a freeze drier [42].
Using a 20 × 100 mm glass column with a 250 mL reservoir, flash column chromatography was carried out. The column was prepared with SiO2 gel infused in hexane. Around 500 mg of the methanol extract of N. biserrata was dissolved in 2 mL of hexane and spiked onto the column once the column settled. The spiked silica gel (SiO2) was eluted with a solvent-gradient Hexane-Ethyl Acetate eluant system; F1-Hex:EtOAc (9:1), F2-Hex:EtOAc (8:2), F3-Hex:EtOAc (7:3), F4-Hex:EtOAc (6:4), F5-Hex:EtOAc (5:5), F6-Hex:EtOAc (4:6), F7-EtOAc (100%) was used to fractionate. A total of seven fractions were collected, each was about 250 mL in volume, fractions were evaporated under vacuo using a vacuum rotary evaporator, before removing the solvent residues using a N2 flushed desiccation. Then, their profiles were determined using thin-layer chromatography to notice their separation. Besides, fractions were subjected to LC-QTOF-MS analysis to determine the inherently present secondary metabolites.

4.4. Antiparasitic Bioassay

The parasitic leeches were isolated from the infested hybrid groupers (Epinephelus fuscoguttatus × E. lanceolatus), Universiti Malaysia Sabah and identified based on its morphological features [7]. The mature leeches were divided into 9 groups (5 leeches per group) and as follows.
Group 1 = negative control, treated with seawater.
Group 2 = positive control, treated with 0.25% formalin solution.
Group 3 = treated with N. biserrata fraction 1 (4.80 mg/mL).
Group 4 = treated with N. biserrata fraction 2 (20 mg/mL).
Group 5 = treated with N. biserrata fraction 3 (2.50 mg/mL).
Group 6 = treated with N. biserrata fraction 4 (14 mg/mL).
Group 7 = treated with N. biserrata fraction 5 (15.30 mg/mL).
Group 8 = treated with N. biserrata fraction 6 (17.20 mg/mL).
Group 9 = treated with N. biserrata fraction 7 (20 mg/mL).
Different concentration of fractions was applied according to the fractionation yield ratio of the methanol extract of N. biserrata to identify the effective fractions against Z. arugamensis. The time limit was recorded until all the leeches were killed after the exposure to various concentrations of chromatographic fractions of methanol extract of N. biserrata [43].

4.5. Behavioural Observation

The behavior of Z. arugamensis was also monitored visually during the exposure to different concentrations of the chromatographic fractions of N. biserrata and compared with normal and positive control groups [43].

4.6. LC-QTOF MS Acquisition

The fractions were analyzed as described previously [22], where Agilent 1290 Infinity LC system coupled with an Agilent 6520 QTOF-MS system was employed. Briefly, a 2.0 µL sample was injected and separated with an Agilent Zorbax Eclipse XDB-C18 column (narrow bore, 2.1 mm × 150 mm × 3.5 µm; Agilent Technologies, Santa Clara, CA, USA) at a flow rate of 0.5 mL/min. Column chamber was maintained at 25 °C during analysis. Solvents A (H2O-0.1% HCOOH) and B (acetonitrile-0.1% HCOOH) were used as the mobile phases, and the gradient was programmed at 5% solvent B from 0 to 5 min, then from 5% to 100% solvent B in 15 min and kept for 5 min, before column re-conditioning as initial for next injection.
Mass spectrometry (MS) data acquisition range was set from m/z of 100 to 1500. Heated electrospray ionization (ESI) was deployed at 4 kV in positive mode. The ion source was set as follows: 300 °C of gas temperature, 10 L/min of drying gas flow, and 45 psig of nebulizer flow. Tuning Mix (Agilent Technologies, Santa Clara, CA, USA) was used for system calibration before analysis. During analysis, internal mass calibration standards betaine and hexakis (1H, 1H, 3H-tetrafluoropropoxy) phosphazine were introduced. In positive mode, the internal mass calibration standards were m/z of 121.0508 and 922.0097, respectively.
Automated tandem mass spectrometry (auto-MS/MS) was performed for metabolites matching, on which two precursor ions were selected from each MS scan and subjected for collision-induced dissociation with 20 eV of collision energy. Data acquisition range for auto-MS/MS was set between an m/z of 50 and 1500, and purified nitrogen gas was used as the collision gas. Acquired data were processed using Agilent MassHunter Qualitative Workflows software (version B.08.00) and the extracted metabolite MS were identified via METLIN metabolites and lipids databases [44] with 2 ppm maximum mass tolerance.

4.7. Statistical Analysis

Data analysis was done via IBM SPSS Statistics 25 Window package (IBM, Armonk, NY, US). One-way variance analysis (ANOVA) followed by Tukey’s multiple comparison test was applied to determine the significant differences between groups All findings were presented as mean ± standard deviation (S.D.). p-Values of less than 0.05 were considered to be significant.

5. Conclusions

In this study, fractions 3, 4 and 5 of N. biserrata demonstrated antiparasitic properties among the seven fractions obtained through flash column chromatography techniques. Significant parasitic mortality was indicated by fraction 3 at a concentration of 2.5 mg/mL, all the parasitic leeches were killed in a time limit of only 1.92 ± 0.59 min. followed by fraction 4 (14 mg/mL) 34.57 ± 3.39 and fraction 5 (15.3 mg/mL) in an average time of 36.82 ± 4.53 min. In a very low concentration (2.5 mg/mL), the methanol extract of N. biserrata could effectively kill the parasites infesting the cultured grouper. LC-QTOF-MS analysis indicated the presence of secondary metabolites including phytosphingosine (6), pyrethrosin (1), haplophytine (9), ivalin (2), warburganal (3), isodomedin (4) and pheophorbide a (16) representing sphingoid, alkaloid, terpenoid, phenolic and flavonoid groups. Thus, our study indicated that N. biserrata fractions contained some effective and potent bioactive compounds with antiparasitic potential.

Supplementary Materials

Table S1: Data of death time and percentage of the fractions of N. biserrata against the marine leeches in replicates.

Author Contributions

M.D.S.: investigation, methodology, data curation, formal analysis, writing original draft, review and editing; K.T.: data curation, formal analysis; Y.S.Y.: data curation, formal analysis, review and editing; F.F.C.: supervision, review and editing; S.R.M.S.: supervision, review and editing; C.S.V.: data curation, formal analysis, review and editing; B.A.V.M.: conceptualization, investigation; methodology, supervision, validation, visualization, writing original draft, review and editing, funding acquisition and project administration. All authors have read and agreed to the published version of the manuscript.

Funding

This study was funded by the Universiti Malaysia Sabah (SDN0073-2019), (PRF No. 0011-2019) and Fundamental Research Grant Scheme, the Ministry of Education Malaysia (grant/award no. FRG0486-2018).

Data Availability Statement

The data presented in this study are available in manuscript and supplementary material.

Conflicts of Interest

The authors declare no conflict of interest.

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Molecules 26 00499 g001 550
Figure 1. Zeylanicobdella arugamensis, arrow indicates both OS = oral or anterior sucker and PS = posterior or caudal sucker. (a) = treated with seawater only, arrow indicated to show the posterior sucker attached firmly to the bottom of the plate, (b) = treated with chemical formalin, here posterior sucker detached, (c,d) = treated with chromatographic fractions of the methanol extract of N. biserrata, showing the detachment of posterior sucker.
Figure 1. Zeylanicobdella arugamensis, arrow indicates both OS = oral or anterior sucker and PS = posterior or caudal sucker. (a) = treated with seawater only, arrow indicated to show the posterior sucker attached firmly to the bottom of the plate, (b) = treated with chemical formalin, here posterior sucker detached, (c,d) = treated with chromatographic fractions of the methanol extract of N. biserrata, showing the detachment of posterior sucker.
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Molecules 26 00499 g002 550
Figure 2. (a) Chromatogram of fraction 3 of N. biserrata. Pyrethrosin (1), phytosphingosine (6), 8-oxo-9,11-octadecadiynoic acid (7), 10,16-heptadecadien-8-ynoic acid (8), haplophytine (9), pheophorbide a (16). (b) Chromatogram of fraction 4. Ivalin (2), 3′-deoxydryopteric acid (5), 5,2′,4′-trihydroxy-3,7,8,5′-tetramethoxyflavone (10), 3,4,5-trimethoxycinnamic acid (12), 9-tetradecenyl acetate (14). (c) Chromatogram of fraction 5. Warburganal (3), isodomedin (4), 3,5,6,7-tetramethoxyflavone (11), 3,4-methylenedioxy-2′,4′-dimethoxychalcone (13), 3,7,17,23-tetrahydroxycholan-24-oic acid (15).
Figure 2. (a) Chromatogram of fraction 3 of N. biserrata. Pyrethrosin (1), phytosphingosine (6), 8-oxo-9,11-octadecadiynoic acid (7), 10,16-heptadecadien-8-ynoic acid (8), haplophytine (9), pheophorbide a (16). (b) Chromatogram of fraction 4. Ivalin (2), 3′-deoxydryopteric acid (5), 5,2′,4′-trihydroxy-3,7,8,5′-tetramethoxyflavone (10), 3,4,5-trimethoxycinnamic acid (12), 9-tetradecenyl acetate (14). (c) Chromatogram of fraction 5. Warburganal (3), isodomedin (4), 3,5,6,7-tetramethoxyflavone (11), 3,4-methylenedioxy-2′,4′-dimethoxychalcone (13), 3,7,17,23-tetrahydroxycholan-24-oic acid (15).
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Molecules 26 00499 g003 550
Figure 3. (a) Chemical structures of the secondary metabolites detected in the chromatographic fraction 3 of N. biserrata. Pyrethrosin (1), phytosphingosine (6), 8-oxo-9,11-octadecadiynoic acid (7), 10,16-heptadecadien-8-ynoic acid (8), haplophytine (9), pheophorbide a (16). (b) Chemical structures of the secondary metabolites detected in the chromatographic fraction 4. Ivalin (2), 3′-deoxydryopteric acid (5), 5,2′,4′-trihydroxy-3,7,8,5′-tetramethoxyflavone (10), 3,4,5-trimethoxycinnamic acid (12), 9-tetradecenyl acetate (14). (c) Chemical structures of the secondary metabolites detected in the chromatographic fraction 5. Warburganal (3), isodomedin (4), 3,5,6,7-tetramethoxyflavone (11), 3,4-methylenedioxy-2′,4′-dimethoxychalcone (13), 3,7,17,23-tetrahydroxycholan-24-oic acid (15).
Figure 3. (a) Chemical structures of the secondary metabolites detected in the chromatographic fraction 3 of N. biserrata. Pyrethrosin (1), phytosphingosine (6), 8-oxo-9,11-octadecadiynoic acid (7), 10,16-heptadecadien-8-ynoic acid (8), haplophytine (9), pheophorbide a (16). (b) Chemical structures of the secondary metabolites detected in the chromatographic fraction 4. Ivalin (2), 3′-deoxydryopteric acid (5), 5,2′,4′-trihydroxy-3,7,8,5′-tetramethoxyflavone (10), 3,4,5-trimethoxycinnamic acid (12), 9-tetradecenyl acetate (14). (c) Chemical structures of the secondary metabolites detected in the chromatographic fraction 5. Warburganal (3), isodomedin (4), 3,5,6,7-tetramethoxyflavone (11), 3,4-methylenedioxy-2′,4′-dimethoxychalcone (13), 3,7,17,23-tetrahydroxycholan-24-oic acid (15).
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Molecules 26 00499 g004 550
Figure 4. Nephrolepis biserrata fern collected from Universiti Malaysia Sabah, scale bar: 5 cm.
Figure 4. Nephrolepis biserrata fern collected from Universiti Malaysia Sabah, scale bar: 5 cm.
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Table
Table 1. Death time and percentage of the fractions of N. biserrata against the marine leeches.
Table 1. Death time and percentage of the fractions of N. biserrata against the marine leeches.
NoGroupDeath Time (min)
Mean ± S.D.		Death Percentage
1Negative Control0 00 ± 0.000
2Positive Control (Formalin 0.025%)3.90 ± 0.84 #100
3Fraction 1 (4.80 mg/mL)0 00 ± 0.00 *0
4Fraction 2 (20 mg/mL)0 00 ± 0.00 *0
5Fraction 3 (2.50 mg/mL)1.92 ± 0.59 # * ^ $100
6Fraction 4 (14 mg/mL)34.57 ± 3.39 # * ^ $ &100
7Fraction 5 (15.30 mg/mL)36.82 ± 4.53 # * ^ $ &100
8Fraction 6 (17.20 mg/mL)0 00 ± 0.00 * & α ®00
9Fraction 7 (20 mg/mL)0 00 ± 0.00 * & β ®00
Values are mean ± SD of six leeches per group. Fraction 1 [hexane: ethyl acetate (H:E) 9:1)]; Fraction 2 (H:E, 8:2); Fraction 3 (H:E, 7:3); Fraction 4 (H:E, 6:4); Fraction 5 (H:E, 5:5); Fraction 6 (H:E, 4:6), Fraction 7 (E, 100%). # Significance at p < 0.05 compared with the negative control group. * Significance at p < 0.05 compared with the formalin [0.25% (v/v)]. ^ Significance at p < 0.05 compared with fraction 1 (4.80 mg/mL). $ Significance at p < 0.05 compared with fraction 2 (20 mg/mL). & Significance at p < 0.05 compared with fraction 3 (2.50 mg/mL). α Significance at p < 0.05 compared with fraction 4 (14 mg/mL). ® Significance at p < 0.05 compared with fraction 5 (15.30 mg/mL). β Significance at p < 0.05 compared with fraction 6 (17.20 mg/mL).
Table
Table 2. Secondary metabolite profiles in fractions of N. biserrata analyzed via LC-QTOF-MS.
Table 2. Secondary metabolite profiles in fractions of N. biserrata analyzed via LC-QTOF-MS.
FractionsRetention Time (RT)Mass to Charge Ratio, m/zFormulaMass Error, ppmMatched MetabolitesClass
113.144203.1043C11 H16 O2−1.393-tert-Butyl-5-methylcatecholPhenolic
212.848231.1377C15 H18 O20.30Isodehydrocostus lactoneTerpenoid
13.112203.1045C11 H16 O2−1.393-tert-Butyl-5-methylcatecholPhenolic
13.443311.1847C17 H26 O5−0.46methyl 8-[2-(2-formyl-vinyl)-3-hydroxy-5-oxo-cyclopentyl]-octanoateFatty acid methyl ester
16.533280.1905C16 H25 N O3−0.37SerratineAlkaloid
19.234425.2905C24 H40 O6−1.51(3α,5β,6α,7α)-2,3,6,7-Tetrahydroxycholan-24-oic acidSteroid
24.443431.3522C28 H46 O3−0.031,25-Dihydroxy-ethylidene-19-norvitamin D3Steroid
312.220318.3001C18 H39 N O30.93Phytosphingosine (6)Sphingoid
14.096307.1541C17 H22 O5−0.38Pyrethrosin (1)Terpenoid
15.081291.1954C18 H26 O3−0.048-oxo-9,11-octadecadiynoic acid (7)Oxo Fatty acid
15.343263.2002C17 H26 O21.1910,16-Heptadecadien-8-ynoic acid (8)Fatty Acid
19.835593.2766C35 H36 N4 O5−0.86Pheophorbide a (16)Chlorophyll breakdown product
20.777653.2969C37 H40 N4 O70.46Haplophytine (9)Alkaloid
410.663333.0965C17 H16 O71.053′-Deoxydryopteric acid (5)Phenolic
11.325391.1018C19 H18 O91.345,2′,4′-Trihydroxy-3,7,8,5′-tetramethoxyflavone (10)Flavonoid
11.404239.0911C12 H14 O50.113,4,5-Trimethoxycinnamic acid (12)Aromatic
14.927277.2138C16 H30 O20.129-Tetradecenyl acetate (14)Aliphatic acetate
15.120249.1483C15 H20 O3−0.32Ivalin (2)Terpenoid
512.796251.1636C15 H22 O31.86Warburganal (3)Terpenoid
13.223313.1070C18 H16 O50.753,4-Methylenedioxy-2′,4′-dimethoxychalcone (13)Aromatic
13.245343.1176C19 H18 O60.273,5,6,7-Tetramethoxyflavone (11)Flavonoid
13.964415.2085C22 H32 O61.05Isodomedin (4)Terpenoid
15.651447.2719C24 H40 O6−1.273,7,17,23-Tetrahydroxycholan-24-oic acid (15)Steroid
68.965465.1018C21 H20 O121.83Herbacetin 3-glucosideFlavonoid
10.052611.1391C30 H26 O141.04Gallocatechin-(4alpha- > 8)-epigallocatechinFlavonoid
12.306290.269C16 H32 O30.2614-hydroxy-hexadecanoic acidFatty acid
13.230343.117C19 H18 O61.783,5,6,7-TetramethoxyflavoneFlavonoid
13.983415.2086C22 H32 O61.55IsodomedinTerpenoid
21.127391.2845C24 H38 O4−0.893,7-Dihydroxychol-22-en-24-oic acidSteroid
72.053328.1393C15 H18 O7−0.86PicrotinTerpenoid
7.065163.0386C9 H6 O31.153-HydroxycoumarinAromatic
7.309355.1016C16 H18 O91.62Chlorogenic AcidPhenolic
8.945465.1020C21 H20 O121.89Herbacetin 3-glucosideFlavonoid
9.008291.0975C14 H14 N2 O5−0.40N2-Malonyl-D-tryptophanAromatic
9.333303.0500C15 H10 O7−1.53MelanoxetinFlavonoid
9.333449.1077C21 H20 O110.25Kaempferol 5-glucosideFlavonoid
10.034404.1341C20 H18 O8−0.71GlucosyloxyanthraquinoneAromatic
12.296290.2687C16 H32 O31.2314-hydroxy-hexadecanoic acidFatty acid
13.994432.2381C24 H30 O6−0.39MagnoshininAromatic
17.923506.2541C30 H32 O6−1.45Rubraflavone DPhenolic
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Shah, M.D.; Tani, K.; Yong, Y.S.; Ching, F.F.; Shaleh, S.R.M.; Vairappan, C.S.; Venmathi Maran, B.A. Antiparasitic Potential of Chromatographic Fractions of Nephrolepis biserrata and Liquid Chromatography-Quadrupole Time-of-Flight-Mass Spectrometry Analysis. Molecules 2021, 26, 499. https://doi.org/10.3390/molecules26020499

AMA Style

Shah MD, Tani K, Yong YS, Ching FF, Shaleh SRM, Vairappan CS, Venmathi Maran BA. Antiparasitic Potential of Chromatographic Fractions of Nephrolepis biserrata and Liquid Chromatography-Quadrupole Time-of-Flight-Mass Spectrometry Analysis. Molecules. 2021; 26(2):499. https://doi.org/10.3390/molecules26020499

Chicago/Turabian Style

Shah, Muhammad Dawood, Kazuki Tani, Yoong Soon Yong, Fui Fui Ching, Sitti Raehanah Muhamad Shaleh, Charles S. Vairappan, and Balu Alagar Venmathi Maran. 2021. "Antiparasitic Potential of Chromatographic Fractions of Nephrolepis biserrata and Liquid Chromatography-Quadrupole Time-of-Flight-Mass Spectrometry Analysis" Molecules 26, no. 2: 499. https://doi.org/10.3390/molecules26020499

APA Style

Shah, M. D., Tani, K., Yong, Y. S., Ching, F. F., Shaleh, S. R. M., Vairappan, C. S., & Venmathi Maran, B. A. (2021). Antiparasitic Potential of Chromatographic Fractions of Nephrolepis biserrata and Liquid Chromatography-Quadrupole Time-of-Flight-Mass Spectrometry Analysis. Molecules, 26(2), 499. https://doi.org/10.3390/molecules26020499

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