Two New Compounds from the Fungus Xylaria nigripes
1
The First Hospital of Hunan University of Chinese Medicine, Center for Medical Research and Innovation, Changsha 410007, China
2
School of Pharmaceutical Sciences, South-Central Minzu University, Wuhan 430074, China
3
Department of Pharmacy, Xiangya Hospital, Central South University, Changsha 410008, China
4
National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, China
*
Authors to whom correspondence should be addressed.
Molecules 2023, 28(2), 508; https://doi.org/10.3390/molecules28020508
Submission received: 28 November 2022
/
Revised: 1 January 2023
/
Accepted: 3 January 2023
/
Published: 4 January 2023
(This article belongs to the Special Issue Natural Products as Inspirations for the Development of Potential Therapeutic Agents)
Abstract
:In the process of discovering more neural-system-related bioactive compounds from Xylaria nigripes, xylariamino acid A (1), a new amino acid derivative, and a new isovaleric acid phenethyl ester (2) were isolated and identified. Their structures and absolute configurations were determined by analyses of IR, HRESIMS, NMR spectroscopic data, and gauge-independent atomic orbital (GIAO) NMR calculation, as well as electronic circular dichroism (ECD) calculation. The isolated compounds were evaluated for their neuroprotective effects against damage to PC12 cells by oxygen and glucose deprivation (OGD). Compounds 1 and 2 can increase the viability of OGD-induced PC12 cells at all tested concentrations. Moreover, compound 2 (1 μmol L−1) can significantly reduce the percentage of apoptotic cells.
1. Introduction
Medicinal fungi are important sources of physiologically beneficial molecules, and many of them are considered to be functional foods. Various types of secondary metabolites with interesting biological activities have been isolated from medicinal fungus, which have the potential to be used as valuable chemical resources for drug discovery [1,2,3]. Xylaria nigripes (family Xylariaceae) is a medicinal and edible fungus with fruiting bodies well known as “Wu Ling Shen” or “Lei Zhen Zi” in China [4,5,6]. As a traditional Chinese medicinal fungus, X. nigripes has been used to treat insomnia, trauma, depression, mental illness, and diseases of the nervous, immune, and endocrine systems [5,6,7,8,9,10,11,12].
Previous investigations on the metabolites of X. nigripes resulted in the isolation of secondary metabolites including alkaloids, sesquiterpenes, steroids, and polyketides, and some of them exhibited promising anti-inflammatory, antioxidant, neuroprotective, and cholesterol ester transfer protein inhibition activities [13,14,15,16,17,18,19]. In our previous research, naphthalenone derivatives and resorcinol derivatives were isolated from X. nigripes, which had potential in protecting PC12 cells from OGD-induced injury in vitro [20,21]. In order to search for secondary metabolites with neuroprotective activity from medicinal fungus, the extract of the rice fermentation of X. nigripes was further investigated. As a result, a new amino acid derivative, named xylariamino acid A (1), and an isovaleric acid phenethyl ester (2) were isolated (Figure 1). The structures and absolute configurations of the new compounds were established unambiguously by spectroscopic data analysis, as well as 13C NMR chemical shifts and electronic circular dichroism (ECD) calculations. Herein, the isolation, structure elucidation, and biological activities of compounds 1 and 2 are described.
2. Results and Discussion
Xylariamino acid A (1) was isolated as a colorless powder. Its molecular formula was determined to be C17H25NO4 by the positive HRESIMS (m/z 330.16748 [M + Na]+, calcd for C17H25NO4Na, 330.16758), indicating six degrees of unsaturation. The 1H NMR spectrum (Table 1) revealed five aromatic protons at δH 7.31 (2H, m, H-2/6), 7.26 (1H, m, H-4), and 7.24 (2H, m, H-3/5), owing to a monosubstituted benzene ring; one oxygenated methylene proton at δH 4.08 (2H, dd, J = 7.2, 6.6 Hz); two methylenes at δH 3.12 (2H, m), 1.46 (1H, m), and 1.58 (1H, m); three methine protons at δH 3.80 (1H, t, J = 6.6 Hz), 3.33 (1H, m), and 1.70 (1H, m); and three methyls at δH 1.12 (3H, t, J = 7.2 Hz), 0.89 (3H, d, J = 6.6 Hz), and 0.86 (3H, d, J = 6.6 Hz). The 13C NMR spectrum displayed 17 carbon resonance signals, which were assigned with the aid of DEPT and HSQC spectroscopy as six aromatic carbons (δC 130.4 × 2, 129.7 × 2, 128.2, 137.4), three methyls (δC 23.0, 22.8, 14.2), three methylenes including one oxygenated resonance (δC 62.7, 42.5, 38.8), three methines (δC 62.6, 60.9, 25.9), and two ester carbonyls (δC 176.5, 173.1).
Further analyses of the 1H–1H COSY and HMBC spectra led to the elucidation of the planar structure of compound 1 (Figure 2). The 1H–1H COSY spectrum indicated the presence of four spin–spin coupling systems: H-2/H-3/H-4/H-5/H-6, H-7/H-8, H-10/H-11, and H-12/H-13/H-14/H-15/H-16. The HMBC correlations of H-11 to C-10, H-10 to C-9, H-8 to C-1, H-7 to C-9, and H-2 to C-7 revealed the presence of a substituted ethyl 3-phenylpropionate unit. The remaining NMR resonances were attributed to an isocaproic acid unit according to the simultaneous HMBC correlations of H3-15/H3-16 to C-13 and C-14, and the key HMBC correlations from H-14 to C-12, as well as from H-13 to C-17. Considering the molecular formula and the downfield chemical shifts of C-12 (δC 60.9) and C-8 (δC 62.6), the two units were deduced to be connected with a nitrogen atom between C-12 and C-8, which was supported by the HMBC correlation from H-12 to C-8. Thus, compound 1 was assigned as an amino acid derivative as shown in Figure 1.
In order to further confirm the relative configuration of compound 1, 13C NMR chemical shift calculations were performed on compound 1 to specify the configurations of C-8 and C-12. All four reasonable configurations of compound 1 were labeled as 8R,12S—1a; 8R,12R—1b; 8S,12S—1c; and 8S,12R—1d (Figure 3), and their chemical shift calculations were performed using the gauge-independent atomic orbital (GIAO) method at the B3LYP-D3(BJ)/TZVP (IEFPCM, CH3OH) level of theory, following a reported STS protocol [20]. The results showed that the calculated data of 1b/1c have the lowest mean absolute error (MAE) and root mean square (RMS) values, and the Pmean and Prel parameters showed that 1b or 1c is the more reasonable configuration (Table 2). The relative configuration of compound 1 was thus established as 8R*,12R*. Moreover, the absolute configuration of compound 1 was determined by a comparison of the experimental ECD and calculated ECD values. The calculated ECD spectrum of 8R,12R—1 showed negative Cotton effects in the wavelength ranging from 200 to 240 nm, which matched well with the experimental curve (Figure 4), allowing the absolute configuration of compound 1 to be determined as 8R,12R. Consequently, the structure of compound 1 was unequivocally established and named xylariamino acid A.
Compound 2 was obtained as yellow oil. Its molecular formula of C13H18O4 was established by the positive HRESIMS ion at m/z 261.1098 [M + Na]+ (calcd for C13H18O4Na, 261.1097), corresponding to five degrees of unsaturation. The 1H NMR spectrum of compound 2 revealed one set of AA’BB’ coupling benzene protons at δH 7.05 (2H, d, J = 8.5 Hz, H-2/6) and 6.72 (2H, d, J = 8.5 Hz, H-3/5); one oxygenated methine proton at δH 3.90 (1H, d, J = 4.8 Hz); a methine proton at δH 1.96 (1H, m); one oxygenated methylene at δH 4.29 (2H, m); one methylene at δH 2.85 (2H, t, J = 6.6 Hz); and two methyl groups at δH 0.92 (3H, d, J = 7.2 Hz) and 0.82 (3H, d, J = 7.2 Hz). The 13C NMR and HSQC spectra displayed 13 carbon resonances, which could be assigned as follows: one ester carbonyl (δC 175.6), one benzene ring (δC 130.9 × 2, 116.2 × 2, 157.1, 129.8), two methines (δC 33.3, 76.6), two methylenes (δC 35.2, 66.8), and two methyls (δC 19.1, 17.0) (Table 1). The benzene ring and ester groups contribute all the five degrees of unsaturation, indicating that compound 2 is a benzene derivative with linear substitutions.
In the HMBC spectrum (Figure 2), the correlations from H-7 to C-2/6, and from H-8 to C-1, together with the 1H–1H COSY cross-peaks of H-7/H-8, established the structure of a 4-hydroxyphenyl ethanol moiety. In addition, the HMBC correlations from H-13 to C-10/C-11/ C-12, and from H-11 to C-9/C-10, along with the analysis of the 1H–1H COSY spectrum indicated the presence of a 2-hydroxyisovaleric acid moiety. Subsequently, the HMBC correlations from H-8 to C-9 verified that the two moieties were connected through an ester bond between the C-8 and C-9. Thus, the planar structure of compound 2 was established as shown in Figure 1. The absolute configuration of compound 2 was established by a comparison of the experimental ECD and calculated ECD data. The results showed that the calculated ECD spectrum of 10R—2 matched well with the negative Cotton effect at the range from 200 to 240 nm in the experimental spectrum (Figure 5). Thus, the structure with the absolute configuration of compound 2 was unequivocally established as 10R.
Compounds 1 and 2 were evaluated for their neuroprotective effects against damage to PC12 cells by OGD. As a result, compounds 1 and 2 increased the viability of OGD-induced PC12 cells at all tested concentrations (Figure 6) and did not exhibit cytotoxicity (Figure S1). As shown in Figure 7, compared with the control group, PC12 cells were treated with hypoxia for 6 h and incubated with or without the test compounds for 24 h, and showed obvious apoptotic morphology. In addition, compared with the model group, pretreatment with nimodipine could reduce early and late apoptotic cells after OGD. Furthermore, compound 2 (1 μmol L−1) could reduce the percentage of apoptotic cells compared with those treated with OGD (Figure 7). Due to the scarcity of the compounds, the neuroprotective activity of compounds 1 and 2 has not been further studied, and more assays (ELISA-based, colorimetric, or cytometry) are need to verify their neuroprotective activity.
3. Materials and Methods
3.1. General Experimental Procedures
UV spectra were afforded with a UH5300 UV-VIS Double Beam Spectrophotometer. ECD spectra were obtained on a Chirascan CD spectrometer (Applied Photophysics, London, UK). HRESIMS spectra were recorded on a Q Exactive Obitrap mass spectrometer (ThermoFisher Scientific, Waltham, MA, USA) and UPLC-ESI-Q-TOF-MS (1290 UPLC-6540, Agilent Technologies Inc., Palo Alto, CA, USA) HRMS spectrometer. IR spectra were obtained with a Shimadzu Fourier Transform Infrared Spectrometer using KBr pellets. NMR spectra were recorded on a Bruker Avance III 600 MHz spectrometer with TMS as an internal standard. Chemical shifts (δ) were expressed in ppm with reference to the solvent signals. Column chromatography (CC) was performed on silica gel (200–300 mesh, Qingdao Marine Chemical Ltd., Qingdao, China) and Sephadex LH-20 (Pharmacia Fine Chemical Co., Ltd., Uppsala, Sweden). Medium Pressure Liquid Chromatography (MPLC) was performed on a Biotage SP1 System and columns packed with RP-18 gel. Preparative high-performance liquid chromatography (prep-HPLC) was performed on an Agilent 1260 liquid chromatography system equipped with Zorbax SB-C18 columns (Agilent, 5 μm, 9.4 mm × 150 mm) and a DAD detector. Fractions were monitored by TLC (GF 254, Qingdao Haiyang Chemical Co., Ltd., Qingdao, China).
3.2. Fungal Material
The fungus Xylaria nigripes (Kl.) Sacc. (Xylariaceae) was collected from Ailao Moutain, Yunnan Province of China in 2013 and was identified by Prof. Yu-Cheng Dai (Beijing Forestry University), a mushroom specialist. The strain of X. nigripes in this study was isolated from the fresh fruiting bodies and kept on a potato, dextrose, and agar (PDA) culture medium. A voucher specimen (No. CGBWSHF00611) was deposited at South-Central Minzu University, China.
3.3. Fermentation and Isolation
This strain of X. nigripes was cultured on potato dextrose agar medium at 25 °C. After eight days, the agar plugs were cut into small pieces to incubate on a solid rice medium in 500 mL Erlenmeyer flasks (100 g rice and 100 mL tap water for each Erlenmeyer flask, the total weight of rice was 7 kg) to culture for a further 30 days at 25 °C. Then, the culture medium and the mycothallus were extracted with ethyl acetate 3 times. The extract was concentrated under vacuum with a rotary evaporator to yield 38 g crude extract.
The crude extract was separated by a silica gel column using petroleum ether and ethyl acetate with a ratio from 1:0 to 0:1 to obtain ten fractions (A–J). Fraction D (8.5 g) was separated by MPLC with a stepwise gradient of MeOH–H2O (0–100%) to afford five fractions (D1–D5). Fr. D2 (0.5 g) was purified by prep-HPLC (MeCN/H2O, v/v, from 25/75 to 35/65 in 20 min, flow speed: 4 mL/min) to give compound 1 (5.8 mg, tR = 16.5 min). Fraction I (4.0 g) was separated by MPLC with a stepwise gradient of MeOH–H2O (0–100%) to afford five fractions (I1–I5). Fr. I2 (0. 8 g) was further separated by Sephadex LH-20 (MeOH) to give eight fractions I2a–I2h. Subfraction I2e (20 mg) was purified by prep-HPLC (MeCN/H2O, v/v, from 20/80 to 30/70 in 20 min, flow speed: 4 mL/min) to give compound 2 (3.9 mg, tR = 14.6 min).
Xylariamino acid A (1): colorless powder; UV (MeOH) λmax(log ε) 210 (1.94) nm; IR (KBr)νmax: 3578, 3379, 1736, 1603, 1379, 1356 cm−1; 1H NMR (600 MHz, methanol-d4) and 13C NMR (150 MHz, methanol-d4) data, see Table 1; HRESIMS: m/z 330.16748 [M + Na]+ (calcd for 330.16758).
Compound (2): yellow oil; UV (MeOH) λmax(log ε) 225 (2.87) nm; IR (KBr)νmax: 3368, 2947, 2835, 1651, 1454, 1418, 1113, 1032 cm−1; 1H NMR (600 MHz, methanol-d4) and 13C NMR (150 MHz, methanol-d4) data, see Table 1; HRESIMS: m/z 261.11012 [M + Na]+ (calcd for 261.10973).
3.4. Cell Viability Assay
A nRPMI-1640 culture medium and phosphate buffer saline (PBS) were purchased from Gibco Co. (Grand Island, NY, USA). Dimethyl sulfoxide (DMSO) and a cell counting kit-8 (CCK-8) were purchased from Boster Biol. Tech (Wuhan, China). An Annexin V-FITC/PI assay kit was purchased from Beyotime Biotechnology, Ltd. (Shanghai, China). Fetal bovine serum (FBS) and PC12 cells were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China). Nimodipine was purchased from Yabao Pharmaceutical Group Co., Ltd. (Shanxi, China).
PC12 cells were seeded into 96-well plates at a density of 1 × 104 cells well-1 and incubated overnight at 37 °C for 24 h. Compounds 1 and 2 were prepared in serial dilutions from 0.01 to 10 µM in a serum-free medium and the cells were treated for 24 h. Then, the medium was replaced with a 10 μL/well CCK8 solution (Bioss, Beijing, China) and the cells were incubated at 37 °C for 1 h. The optical density (OD) was measured at 450 nm by a microplate reader (Enspire, PerkinElmer, Waltham, MA, USA). The neuroprotective activity of the tested compounds was assayed in 96-well plates, according to the methods in the previously published article [18].
3.5. Cell Apoptosis Assay
The apoptosis of PC12 cells was detected using the Annexin V-FITC/PI (Beyotime Biotechnology, Shanghai, China). The logarithmically growing human neuroblastoma PC-12 cells were plated at a density of 8 × 104 cells well-1 in 24-well plates for 24 h. Then, cells were treated with hypoxia for 6 h, and then incubated with or without the test compounds for 24 h. A single adherent culture of PC-12 cells was washed with PBS, then the cells were evenly mixed with 195 µL 1× binding buffer, 10 µL Annexin V-FITC, and 10 µL PI and incubated for 15 min in the dark. The apoptosis data were analyzed by ZEN software (Zesis, Jena, Germany).
3.6. Computation Methods
The software Crestwas was used to search the conformers of compound 1 on the GFNFF level of theory [21,22], followed by optimization on the GFN2-XTB level with a 4 kcal/mol energy window to remove high energy conformers [23]. The optimization and frequency calculation of each conformer was performed on the B3LYP-D3(BJ)/TZVP (IEFPCM, CH3OH) level of theory. The DFT GIAO 13C NMR calculation was calculated on the ωB97xD/6-31G* (IEFPCM, CHCl3) level, and the data processing followed the reported STS protocol [20]. The calculated shielding tensors of the conformers were Boltzmann-averaged based on Gibbs free energy. The theoretical ECD was calculated by time-dependent density functional theory (TDDFT) at the mPW1PW91/6-311g(d) level with an IEF-PCM solvent model (MeOH) as well. SpecDis v1.71 was used to simulate the ECD curve with a sigma/gamma value of 0.35 eV. The calculated ECD curve of each conformer was Boltzmann-averaged based on their Gibbs free energy. All DFT calculations were performed by the Gaussian 16 software package.
4. Conclusions
In this work, two new compounds were isolated from the cultures of X. nigripes. The structures and absolute configurations of the isolates were unambiguously identified by a detailed interpretation of the NMR spectroscopic and mass spectrometric data, 13C NMR chemical shift, and ECD calculations. The neuroprotective activity test of compounds 1 and 2 showed that both of them can increase the viability of OGD-induced PC12 cells at all tested concentrations. Moreover, compound 2 could reduce the percentage of apoptotic cells at the concentration of 1 μmol L−1, which suggested that it may have potential neuroprotective activity.
Supplementary Materials
The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/molecules28020508/s1, Figure S1: Cell viability of normal PC12 cells with different concentrations of compounds 1 and 2; Figure S2: The cell viability of DMSO group with different concentrations of compounds 1 and 2; Figure S3: The apoptosis rate of PC12 cells with different groups; Figures S4–S19: HRESIMS and NMR spectra of compounds 1 and 2; Table S1: Cell viability of normal PC12 cells with different concentrations of compounds 1 and 2; Table S2: Cell viability of PC12 cells evaluated by the CCK8 test (** p < 0.01 compared with control group, ## p < 0.01 compared with model group); Table S3: Cell viability of the tested compounds 1 and 2 compared with DMSO group (* p < 0.05, ** p < 0.01); Table S4: The neuronal apoptosis rate was presented as the percentage of Annexin FITC/PI-positive neurons. The percentage of Annexin FITC/PI-positive neurons was calculated by dividing the number of Annexin FITC/PI-positive neurons with the total number of neurons. Data are presented as the mean ± SD. ** p < 0.01 vs. Control group; ## p < 0.01 vs. Model group; Table S5: Geometry data of conformers of structure 1a; Table S6: Geometry data of conformers of structure 1b.
Author Contributions
Conceptualization, J.L. (Jikai Liu) and J.L. (Jing Li); methodology, J.L. (Jing Li) and H.L.; software, J.L. (Jing Li); validation, J.L. (Jikai Liu), J.L. (Jing Li), and H.L.; formal analysis, J.L. (Jing Li); investigation, J.L. (Jing Li) and L.L.; resources, J.L. (Jing Li); data curation, J.L. (Jing Li) and S.Z.; writing—original draft preparation, J.L. (Jing Li) and H.L.; writing—review and editing, J.L. (Jing Li); visualization, J.L. (Jing Li); supervision, J.L. (Jing Li); project administration, J.L. (Jikai Liu); funding acquisition, J.L. (Jing Li). All authors have read and agreed to the published version of the manuscript.
Funding
This research was funded by the National Natural Science Foundation of China (No. 81903513) and China Postdoctoral Science Foundation (No. 2020M682599).
Institutional Review Board Statement
Not applicable.
Informed Consent Statement
Not applicable.
Data Availability Statement
All data are available in this publication and in the Supplementary Materials.
Acknowledgments
We are grateful for the convenience for the HRMS and NMR measurement provided by the Analytical & Measuring Center, School of Pharmaceutical Sciences, South-Central Minzu University.
Conflicts of Interest
The authors declare no conflict of interest.
Sample Availability
Samples of the compounds are available from the authors.
References
- Chen, M.-C.; Wang, G.-J.; Kuo, Y.-H.; Chiang, Y.-R.; Cho, T.-Y.; Ju, Y.-M.; Lee, T.-H. Isoprenyl phenolic ethers from the termite nest-derived medicinal fungus Xylaria fimbriata. J. Food Drug Anal. 2019, 27, 111–117. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Tang, Y.; Zhao, Z.-Z.; Feng, T.; Li, Z.-H.; Chen, H.-P.; Liu, J.-K. Triterpenes with unusual modifications from the fruiting bodies of the medicinal fungus Irpex lacteus. Phytochemistry 2019, 162, 21–28. [Google Scholar] [CrossRef] [PubMed]
- Chen, M.-C.; Cho, T.-Y.; Kuo, Y.-H.; Lee, T.-H. Meroterpenoids from a Medicinal Fungus Antrodia cinnamomea. J. Nat. Prod. 2017, 80, 2439–2446. [Google Scholar] [CrossRef] [PubMed]
- Yang, X.L.; Liu, J.K.; Luo, D.Q.; Zhang, S. Chemical Constituents of Xylaria nigripe. Nat. Prod. Res. Dev. 2011, 23, 846–849. [Google Scholar]
- Lu, Y.; Lu, H.; Qin, D. The clinical effect of Wuling capsule on wild depression. Chin. Trad. Patent Med. 2010, 32, 1083–1084. [Google Scholar]
- Song, X.; He, J.; Zheng, T.; Ye, R.; Yuan, Z. Research on treatment effects of Wuling capsule for sub-healthy state insomnia. Chin. Arch. Tradit. Chin. Med. 2010, 28, 477–478. [Google Scholar]
- Lin, Y.; Wang, X.-Y.; Ye, R.; Hu, W.-H.; Sun, S.-C.; Jiao, H.-J.; Song, X.-H.; Yuan, Z.-Z.; Zheng, Y.-Y.; Zheng, G.-Q.; et al. Efficacy and safety of Wuling capsule, a single herbal formula, in Chinese subjects with insomnia: A multicenter, randomized, double-blind, placebo-controlled trial. J. Ethnopharmacol. 2013, 145, 320–327. [Google Scholar] [CrossRef]
- Zhao, Z.; Li, Y.; Chen, H.; Huang, L.; Zhao, F.; Yu, Q.; Xiang, Z.; Zhao, Z. Xylaria nigripes mitigates spatial memory impairment induced by rapid eye movement sleep deprivation. Int. J. Clin. Exp. Med. 2014, 7, 356–362. [Google Scholar]
- Peng, W.-F.; Wang, X.; Hong, Z.; Zhu, G.-X.; Li, B.-M.; Li, Z.; Ding, M.-P.; Geng, Z.; Jin, Z.; Miao, L.; et al. The anti-depression effect of Xylaria nigripes in patients with epilepsy: A multicenter randomized double-blind study. Seizure 2015, 29, 26–33. [Google Scholar] [CrossRef] [Green Version]
- Divate, R.D.; Chung, Y.-C. In vitro and in vivo assessment of anti-inflammatory and immunomodulatory activities of Xylaria nigripes mycelium. J. Funct. Foods 2017, 35, 81–89. [Google Scholar] [CrossRef]
- Divate, R.D.; Wang, P.-M.; Wang, C.-C.; Chou, S.-T.; Chang, C.-T.; Chung, Y.-C. Protective effect of medicinal fungus Xylaria nigripes mycelia extracts against hydrogen peroxide-induced apoptosis in PC12 cells. Int. J. Immunopathol. Pharmacol. 2017, 30, 105–112. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Wang, J.L.; Guo, R.J.; Wang, Y.L. Review on pharmacological effects and clinical application of Wuling shen. Global Trad. Chin. Med. 2010, 13, 150–152. [Google Scholar]
- Li, M.; Xiong, J.; Huang, Y.; Wang, L.-J.; Tang, Y.; Yang, G.-X.; Liu, X.-H.; Wei, B.-G.; Fan, H.; Zhao, Y.; et al. Xylapyrrosides A and B, two rare sugar-morpholine spiroketal pyrrole-derived alkaloids from Xylaria nigripes: Isolation, complete structure elucidation, and total syntheses. Tetrahedron 2015, 71, 5285–5295. [Google Scholar] [CrossRef]
- Xiong, J.; Huang, Y.; Wu, X.-Y.; Liu, X.-H.; Fan, H.; Wang, W.; Zhao, Y.; Yang, G.-X.; Zhang, H.-Y.; Hu, J.-F. Chemical Constituents from the Fermented Mycelia of the Medicinal Fungus Xylaria nigripes. Helv. Chim. Acta 2016, 99, 83–89. [Google Scholar] [CrossRef]
- Hu, D.; Li, M. Three New Ergot Alkaloids from the Fruiting Bodies of Xylaria nigripes (KL.) SACC. Chem. Biodivers. 2017, 14, e1600173. [Google Scholar] [CrossRef] [PubMed]
- Chang, J.-C.; Hsiao, G.; Lin, R.-K.; Kuo, Y.-H.; Ju, Y.-M.; Lee, T.-H. Bioactive Constituents from the Termite Nest-Derived Medicinal Fungus Xylaria nigripes. J. Nat. Prod. 2017, 80, 38–44. [Google Scholar] [CrossRef]
- Liaw, C.-C.; Wu, S.-J.; Chen, C.-F.; Lai, M.-N.; Ng, L.T. Anti-Inflammatory Activity and Bioactive Constituents of Cultivated Fruiting Bodies of Xylaria nigripes (Ascomycetes), a Chinese Medicinal Fungus. Int. J. Med. Mushrooms 2017, 19, 915–924. [Google Scholar] [CrossRef]
- Li, J.; Li, L.-Q.; Long, H.-P.; Liu, J.; Jiang, Y.-P.; Xue, Y.; Wang, W.-X.; Tan, G.-S.; Gong, Z.-C.; Liu, J.-K. Xylarinaps A–E, five pairs of naphthalenone derivatives with neuroprotective activities from Xylaria nigripes. Phytochemistry 2021, 186, 112729. [Google Scholar] [CrossRef]
- Li, L.-Q.; Li, J.; Long, H.-P.; Liu, J.-K.; Wang, X. Four new resorcinol derivatives with neuroprotective activities from Xylaria nigripes. Nat. Prod. Res. 2022, 36, 1522–1528. [Google Scholar] [CrossRef]
- Li, J.; Liu, J.-K.; Wang, W.-X. GIAO 13C NMR Calculation with Sorted Training Sets Improves Accuracy and Reliability for Structural Assignation. J. Org. Chem. 2020, 85, 11350–11358. [Google Scholar] [CrossRef]
- Pracht, P.; Bohle, F.; Grimme, S. Automated exploration of the low-energy chemical space with fast quantum chemical methods. Phys. Chem. Chem. Phys. 2020, 22, 7169–7192. [Google Scholar] [CrossRef] [PubMed]
- Spicher, S.; Grimme, S. Robust Atomistic Modeling of Materials, Organometallic, and Biochemical Systems. Angew. Chem. Int. Ed. 2020, 59, 15665–15673. [Google Scholar] [CrossRef] [PubMed]
- Bannwarth, C.; Ehlert, S.; Grimme, S. GFN2-xTB an accurate and broadly parametrized self-consistent tight-binding quantum chemical method with multipole electrostatics and density-dependent dispersion contributions. J. Chem. Theory Comput. 2019, 15, 1652–1671. [Google Scholar] [CrossRef] [PubMed]
Figure 1.
Structures of compounds 1 and 2.
Figure 2.
Key 2D NMR correlations of compounds 1 and 2.
Figure 3.
Four possible relative configurations of compound 1.
Figure 4.
Experimental and calculated ECD spectra of compound 1.
Figure 5.
Experimental and calculated ECD spectra of compound 2.
Figure 6.
Effects of compounds 1 and 2 on cell viability in OGD-induced PC12 cells. The values represent mean ± SD (n = 6). ** p < 0.01 vs. the control group; ## p < 0.01 vs. the model group.
Figure 6.
Effects of compounds 1 and 2 on cell viability in OGD-induced PC12 cells. The values represent mean ± SD (n = 6). ** p < 0.01 vs. the control group; ## p < 0.01 vs. the model group.
Figure 7.
Annexin V-FITC/PI staining and fluorescence microscopy images (original magnification, 200×) (PC12 cells treated with 1 μmol L−1 of compounds 1 and 2).
Figure 7.
Annexin V-FITC/PI staining and fluorescence microscopy images (original magnification, 200×) (PC12 cells treated with 1 μmol L−1 of compounds 1 and 2).
Table 1.
1H NMR (600 MHz) and 13C NMR (150 MHz) data of compounds 1 and 2 in methanol-d4.
| No. | 1 | 2 | ||
|---|---|---|---|---|
| δH | δC | δH | δC | |
| 1 | 137.4 | 129.8 | ||
| 2 | 7.31, m | 129.7 | 7.05, d (8.4) | 130.9 |
| 3 | 7.24, m | 130.4 | 6.72, d (8.4) | 116.2 |
| 4 | 7.26, m | 128.2 | 157.1 | |
| 5 | 7.24, m | 130.4 | 6.72, d (8.4) | 116.2 |
| 6 | 7.31, m | 129.7 | 7.05, d (8.4) | 130.9 |
| 7 | 3.12, m | 38.8 | 2.85, t (6.6) | 35.2 |
| 8 | 3.80, t (6.6) | 62.6 | 4.29, m | 66.8 |
| 9 | 173.1 | 175.6 | ||
| 10 | 4.08, dd (7.2,6.6) | 62.7 | 3.90, d (4.8) | 76.6 |
| 11 | 1.12, t (7.2) | 14.2 | 1.96, m | 33.3 |
| 12 | 3.33, m | 60.9 | 0.92, d (7.2) | 19.1 |
| 13 | 1.46, m, 1.58, m | 42.5 | 0.82, d (7.2) | 17.0 |
| 14 | 1.70, m | 25.9 | ||
| 15 | 0.89, d (6.6) | 22.8 | ||
| 16 | 0.86, d (6.6) | 23.0 | ||
| 17 | 176.5 | |||
Table 2.
Calculated 13C chemical shifts of 1a–1d fitting with the experimental data of compound 1 following STS protocol.
Table 2.
Calculated 13C chemical shifts of 1a–1d fitting with the experimental data of compound 1 following STS protocol.
| NO. | Exptl. ΔC | Calcd. ΔC 1a/1d | Dev. | Calcd. ΔC 1b/1c | Dev. |
|---|---|---|---|---|---|
| 1 | 128.2 | 127.52 | 0.68 | 127.36 | 0.84 |
| 2 | 130.4 | 129.05 | 1.35 | 128.91 | 1.49 |
| 3 | 129.7 | 128.98 | 0.72 | 129.30 | 0.40 |
| 4 | 137.4 | 137.72 | 0.32 | 137.05 | 0.35 |
| 5 | 129.7 | 128.98 | 0.72 | 129.30 | 0.40 |
| 6 | 130.4 | 129.05 | 1.35 | 128.91 | 1.49 |
| 7 | 38.8 | 40.17 | 1.37 | 42.23 | 3.43 |
| 8 | 62.6 | 66.37 | 3.77 | 64.28 | 1.68 |
| 9 | 173.1 | 175.48 | 2.38 | 176.41 | 3.31 |
| 10 | 62.7 | 63.74 | 1.04 | 62.73 | 0.03 |
| 11 | 14.2 | 14.85 | 0.65 | 14.97 | 0.77 |
| 12 | 60.9 | 61.59 | 0.69 | 60.30 | 0.60 |
| 13 | 42.5 | 41.01 | 1.49 | 40.88 | 1.62 |
| 14 | 25.9 | 23.97 | 1.93 | 25.62 | 0.28 |
| 15 | 22.8 | 22.28 | 0.52 | 22.21 | 0.59 |
| 16 | 23 | 22.28 | 0.72 | 22.21 | 0.79 |
| 17 | 176.5 | 175.77 | 0.73 | 176.12 | 0.38 |
| MAE | 1.20 | MAE | 1.09 | ||
| RMS | 1.46 | RMS | 1.45 | ||
| Pmean | 27.01% | Pmean | 31.30% | ||
| Prel | 7.56% | Prel | 92.44% |
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. |
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Share and Cite
MDPI and ACS Style
Long, H.; Zhou, S.; Li, L.; Li, J.; Liu, J. Two New Compounds from the Fungus Xylaria nigripes. Molecules 2023, 28, 508. https://doi.org/10.3390/molecules28020508
AMA Style
Long H, Zhou S, Li L, Li J, Liu J. Two New Compounds from the Fungus Xylaria nigripes. Molecules. 2023; 28(2):508. https://doi.org/10.3390/molecules28020508
Chicago/Turabian StyleLong, Hongping, Siqian Zhou, Lanqing Li, Jing Li, and Jikai Liu. 2023. "Two New Compounds from the Fungus Xylaria nigripes" Molecules 28, no. 2: 508. https://doi.org/10.3390/molecules28020508
APA StyleLong, H., Zhou, S., Li, L., Li, J., & Liu, J. (2023). Two New Compounds from the Fungus Xylaria nigripes. Molecules, 28(2), 508. https://doi.org/10.3390/molecules28020508
