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Review

Exosome: A Novel Approach to Stimulate Bone Regeneration through Regulation of Osteogenesis and Angiogenesis

by
Yunhao Qin
1,
Ruixin Sun
2,
Chuanlong Wu
3,
Lian Wang
4 and
Changqing Zhang
1,*
1
Department of Orthopaedics, Shanghai Jiaotong University Affiliated Sixth People’s Hospital, Shanghai 200030, China
2
The Key Laboratory of Molecular Medicine, the Ministry of Education, Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai 200032, China
3
Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedics Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China
4
School of Medicine, Shanghai Tongji University School of Medicine, Shanghai 200092, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2016, 17(5), 712; https://doi.org/10.3390/ijms17050712
Submission received: 31 March 2016 / Revised: 22 April 2016 / Accepted: 5 May 2016 / Published: 19 May 2016
(This article belongs to the Special Issue Focus on Extracellular Vesicles)
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Abstract

:
The clinical need for effective bone regeneration therapy remains in huge demands. However, the current “gold standard” treatments of autologous and allogeneic bone grafts may result in various complications. Furthermore, safety considerations of biomaterials and cell-based treatment require further clarification. Therefore, developing new therapies with stronger osteogenic potential and a lower incidence of complications is worthwhile. Recently, exosomes, small vesicles of endocytic origin, have attracted attention in bone regeneration field. The vesicles travel between cells and deliver functional cargoes, such as proteins and RNAs, thereby regulating targeted cells differentiation, commitment, function, and proliferation. Much evidence has demonstrated the important roles of exosomes in osteogenesis both in vitro and in vivo. In this review, we summarize the properties, origins and biogenesis of exosomes, and the recent reports using exosomes to regulate osteogenesis and promote bone regeneration.

Graphical Abstract

1. Introduction

In the clinic, lack of bone regeneration may result in a poor prognosis, even in common bone fractures. It has been reported that approximately 2% to 10% of bone fractures may develop non-union due to insufficient bone growth [1], and patients suffer from disabilities and may even have a shorter life expectancy as a result. The surgical removal of carcinomas is another major cause of insufficient bone regeneration, especially in patients with cancer-related bone metastasis [2]. Additionally, a higher incidence of obesity results in more pronounced musculoskeletal illnesses and decreased bone regeneration [3]. The ageing of the population exacerbates this situation. Aging not only results in a higher bone fracture risk due to loss of bone density but also diminishes the capability of bone regeneration [4]. Therefore, how to promote bone regeneration, especially in patients with large bone defects, remains a major challenge for the clinicians.
The current “gold standard” treatment in the clinical settings promotes bone regeneration through the use of autologous and allogeneic bone grafting. However, approximately 20%–30% of patients who undergo autologous bone grafts suffer from morbidity at the graft-harvesting site [5]. Moreover, autologous bone grafts cannot provide patients with large defects with sufficient bones [6]. After an allogeneic bone grafts, over 30% of patients suffer from complications, including fracture, non-union, and infection [7]. Allogeneic bone grafts may also result in graft-versus-host disease (GVHD) [8]. In addition, in cases in which patients received successful bone grafts, the recovery is time-consuming, up to one and half years [9]. Therefore, neither of these two options is the optimal, because they are expensive, uncomfortable for the patients, and have high risks of complication.
Biomaterials and the cell-based therapies are two major research fields in bone regeneration. However, there are some drawbacks to both treatments. The toxicity and immunogenicity of biomaterials may culminate in severe complications. Cell-based therapy is closely related to tumor and emboli formation [10]. Today, exosomes, ranging in size from 50–120 nm [11], with fewer safety considerations and powerful pro-osteogenesis abilities, provide researchers with a novel way to stimulate bone regeneration. This type of vesicles is endocytic origin and released by various cells and organs. Exosomes deliver various content [12], including DNAs, RNAs and proteins and are widely distributed, and are especially enriched in breast milk, semen, saliva, urine and sputum [13]. Exosomes can effectively stimulate regeneration in tissues and organs, including the heart, lung, liver and kidney. Small vesicles can also stimulate bone regeneration in vitro and in vivo. The good bone specificity and powerful bone regenerative properties make exosomes a potential treatment to enhance bone growth and to treat clinical bone diseases.

2. Bone Regeneration Requires the Coordination of Various Cells

Understanding bone biogenesis is a precondition for developing novel approaches to stimulate bone regeneration. Osteoblasts, osteoclasts and chondrocytes are the major cells involved in bone regeneration. The bone may regenerate in two different ways: by intramembranous ossification or endochondral ossification [14]. Intramembranous ossification gives rise to flat bones and endochondral ossification gives rise to long bone. Both ossifications begin with condensation of the mesenchyme and culminate in the formation of calcified bone. However, intramembranous ossification achieves bone calcification directly by mesenchymal stem cell (MSC) osteoblastic differentiation, whereas endochondral ossification incorporates a complicated step in which chondrocytes regulate the growth and formation of the skeleton [15]. During endochondral ossification, chondrocytes in the center of the cartilage stop proliferating and start to enlarge (hypertrophy), while synthesizing and releasing type X collagen [16]. Hypertrophic chondrocytes undergo mineralization and induce vessel penetration and osteoblast differentiation and migration. The recruited endothelial cells secrete vascular endothelial growth factor (VEGF) and direct chondroclasts to digest the surrounding matrix. Finally, hypertrophic chondrocytes undergo apoptotic cell death, and blood vessels and osteoblasts infiltrate the cartilage matrix and subsequently achieve bone growth and regeneration.
In addition to osteoblasts, osteoclasts and chondrocytes, endothelial cells also have a large influence on bone regeneration. Endothelial cells stimulate osteoblast maturation and activity but inhibit the osteoblastic differentiation of osteoprogenitor cells [17]. Additionally, successful bone regeneration is closely related to successful angiogenesis, and impaired angiogenesis always results in failed bone regeneration. Inhibition of VEGF, a potent angiogenesis factor, results in the abnormal endochondral bone formation [18]. Endothelial cell-specific Notch knock-out not only impairs angiogenesis but also reduces osteogenesis, bone length and bone mass [19,20]. All of this evidence indicates that endothelial cells play important roles in bone regeneration.
Previous bone regeneration studies have mainly focused on stimulating the function of cells, and cell-to-cell communication has not been well studied. However, previous research has shown that cell-to-cell communication also greatly affects bone regeneration. Therefore, a therapy that could greatly increase osteogenic cell bone formation and the interaction between cells would be a potential future treatment. Exosomes, nanoscale vesicles ranging from 50–120 nm [11], have both properties, which has prompted intensive investigation of the exosomes.

3. The Properties of Exosomes

Exosomes were first obtained from cell lines and described in 1981 [21], as exfoliated vesicles with ectoenzyme activities. The current definition is that they are membrane vesicles of endocytic origin [22] and are released into the extracellular environment upon the fusion with the plasma membrane (Figure 1) [23]. Exosomes are nanoscale vesicles ranging in size from 50–120 nm [11], with a density in sucrose of 1.13–1.19 g/mL and are wildly distributed both in vivo and in vitro [24] (Table 1). Many cells, including reticulocytes [24], dendritic cells [25], B cells [26], T cells [27], mast cells [28], epithelial cells [29] and tumor cells [30], secrete exosomes. Exosomes transport coding RNA [31], noncoding RNA [32], proteins [33], antigen presentation molecules [34], and DNA [35] between cells. By conveying proteins and RNAs, exosomes modulate the recipient cells and other organs function, activity, and commitment of recipient cells and other organs over a long distance [36].
Although how exosomes participate in cell-to-cell communication is not fully understood, several studies have revealed that ligand–receptor interaction plays an important role in this process [37]. This hypothesis was first proposed by Raposo, et al. [26], who noted that exosomes from B cells incorporate and transport functional antigen-presenting complexes. This discovery suggests that the mechanism of exosome participation in cell-to-cell communication involves receptor–ligand interactions [38]. The vesicles attach or fuse with the target cell membrane via exosome surface proteins such as Alix or Tumor Susceptibility 101 (TSG101), and tetraspanins such as CD9, CD63, CD81 and CD82 [39,40].
Although the cargoes transported by exosomes are diverse, exosomal proteins and RNAs are believed to play important roles in regulating the function of recipient cells [41]. Spectrometry data for exosomes have identified over 4000 different proteins in exosomes [11]. Though exosomal proteins differ substantially according to the origin of the exosome and have different functions, some proteins are shared by all types of exosomes [42]. These commonly-shared proteins are cell-to-cell communication related. For example, heat shock protein (HSP) 70 and HSP90 are shared by all exosomes and are key to protein trafficking [43]. Annexin is a membrane trafficking protein that is involved in fusion events and is enriched in exosomes. Additionally, the cytoskeletal proteins, including myosin, actin and tubulin, are found in exosomes. Regarding another potential functional content in exosomes, small RNAs have attracted the most attention [44,45]. Koppers-Lalic et al. [41] have provided a review of exosomal RNAs and have noted that the functional exosomal RNAs are critical in the regulation of cell commitment, differentiation, and activity.

4. Exosomes Promote Regeneration in Various Tissues though Functional Cargo Transportation

The regenerative effect of exosomes has been validated in other tissues and organs, including the heart [37], lung [46], kidney [47] and brain [48]. Organ functions benefit from exosome treatment. In myocardial infraction mouse models, ventricular remolding and the left ventricular ejection fraction have been found to significantly improved after exosome treatment [37]. Exosomes also mediate cell function, thus, promoting regeneration. In hypoxia-induced pulmonary hypertension mice, exosomes treatment inhibits disease progression and protected the lung from hypertension through a MSC cytoprotective action. Furthermore, exosomes not only prevent apoptosis but also strengthen cell endurance. The renal injury is less severe, and exosome treatment improves renal function in mice with acute kidney injury [47]. In addition, modification of exosomes may provide a more effective treatment of diseases. Alvarez-Ervitl et al. [48] have demonstrated amelioration of Alzheimer’s disease through the injection of exosomes secreted from modified cells. Basu et al. [49] have reviewed of current exosomal treatment in neuroregeneration and skin regeneration.
These studies have provided a foundation for exosome treatment and the exploration of the roles of exosomes in bone regeneration. Recent studies have demonstrated that exosome treatment stimulates bone regeneration in vivo and in vitro. Although the outcome of exosome treatment is inspiring, the exact underlying mechanism remains elusive.

5. Exosomes Regulate Mesenchymal Stem Cell Osteogenic Differentiation

Exosomes directly regulate and guide MSCs into the osteoblastic lineage. MSC-derived exosomes can be used as biomimetic tools to induce naïve stem cells into to a osteogenic linage [50]. Profiling data for the MSC-derived exosome have revealed that nine miRNAs (let-7a, miR-199b, miR-218, miR-148a, miR-135b, miR-203, miR-219, miR-299-5p and miR-302b) are up-regulated and four miRNAs (miR-221, miR-155, miR-885-5p, miR-181a and miR-320c) are down-regulated during the process of MSC osteoblastic differentiation [51]. All of these miRNAs have roles in osteoblast function and activity. This profiling provides preconditions for further investigation and application of MSC-derived exosomes. However, the osteoblast itself also secretes exosomes, thus establishing a positive feedback of bone growth. Mineralizing osteoblast-derived exosome greatly increases osteoblastic differentiation related miRNAs, and activate Wnt signaling via Axin1 inhibition, thereby promoting MSC osteogenic differentiation [52]. Furthermore, eukaryotic initiation factor 2 in osteoblast-derived exosomes may also induce MSC to differentiate into osteoblast [53]. As is well known, the immune system and the hematopoietic system have a strong influence on bone growth, although the exact mechanism remains elusive. Exosomes may contribute to this process. Studies have demonstrated that dendritic cell- [54] and monocyte [55] cell-derived exosomes significantly stimulate MSC osteogenic differentiation in vitro by delivery of exosomal miRNAs (Table 2). MSC osteogenic differentiation is under the regulation of exosomes; however, which cell type-derived exosome is the most potent regulator and how the exosomes mediate MSC differentiation remain to be investigated.

6. Exosomes Regulate Osteoblast Proliferation and Activity

It is known that 4%–6% of the total resident cells in the bone are osteoblasts, whose major function is bone formation [56]. During bone formation, osteoblasts produce calcium- and phosphate-based minerals to form mineralized bone. Exosomes can also stimulate bone regeneration by directly regulating osteoblast proliferation and activity. Prostate cancer cell-derived exosomes increase human osteoblast proliferation by 1.5-fold [57], whereas matrix-derived exosome-treated osteoblasts generate more calcium deposits and greater ALP activity [58]. Prostate cancer cell-derived exosomes showed an excellent bone affinity [57]. Most injected PKH2-labeled exosomes enter the lung and the bone marrow within 24 h, and little is found in other organs. Whether other cell-type-derived exosomes share the same distribution remains unknown. The in vivo influence of exosomes on osteoblasts is also significant. Rats with calvarial defects benefit from bone marrow stromal cell-derived exosomes and show an earlier healing of defects [59] (Table 2). The exosomal miRNA-196a is the key factor stimulating the proliferation and activity of osteoblasts. Although both in vivo and in vitro studies underscore the importance of exosomes to osteoblast, more information is needed regarding the exact exosome treatment efficacy in bone systems.

7. Exosomes Regulate Osteoclast Maturation and Activity

As is well known, bone metastasis is closely related to the abnormal activation of osteoclasts. Research has shown that tumor cells induce osteolysis by secreting vesicles to increase the number and activity of mature osteoclasts. For example, multiple myeloma-derived exosomes internalized by the Raw264.7 cell lines and human primary osteoclast, increased expression of osteoclast marker, including Cathepsin K, Metalloproteinases 9, and Tartrate-resistant Acid Phosphatase (TRAP), thus promoting the maturation of osteoclasts [60]. In prostate cancer cell-derived exosomes, the vesicles also increase osteoclastogenesis by stimulating receptor activator of nuclear factor κB (RANK) expression [57].
In fact, the bone system itself is the most important regulator of osteoclast differentiation. Osteoblast- and osteocyte-derived lysosomal membrane protein 1 (LAMP1) positive exosomes also contain TRAP, RANK ligand, and osteoprotegerin (OPG), which are critical to osteoclast differentiation [61]. However, mature osteoclasts may regulate the cells themselves through exosome secretion. The profiles of osteoclast-derived exosomes indicate that RANK is highly enriched. The depletion of RANK-enriched exosomes results in inhibition of osteoclast formation [62] (Table 2). The roles of exosomes in osteoclasts may provide hints as to how bone formation and absorption are orchestrated.

8. Exosomes Are Potent Pro-Angiogenic Factors

Although there are no direct studies of the angiogenic ability of exosomes in bone, exosomes stimulate angiogenesis in other tissues and organs. The potent exosomal angiogenic ability may possibly stimulate bone growth and regeneration by increasing vessel formation. It has been demonstrated that exosomes stimulate endothelial cell proliferation, migration, and tube formation in vitro, such as placental MSC-derived exosomes [63]. Furthermore, exosomes also increase endothelial cell migration and tube formation through transportation of functional enzymes, including subunit of NADH oxidase [64], metalloproteinases and extracellular matrix metalloproteinase inducer [65]. Moreover, exosomes promote endothelial cell proliferation and vessel formation through exosomal miR-129, miR-136 [66] and the miR-17-92 cluster [63]. Not only do exosomes show angiogenic potency in vitro, but they also enhance angiogenesis in vivo. Research has shown that MSC-derived exosomes successfully improved angiogenesis in different animal models. For example, tail injection of MSC derived exosomes reduces myocardial ischemic/reperfusion injury and improves angiogenesis in the ischemic heart [67], and umbilical cord derived-MSC exosomes from human promotes blood perfusion and attenuated hind-limb ischemia [68] (Table 2). Exploring the roles that exosomes play in bone vessel formation is expected to lead to the development of novel treatments for bone regeneration.

9. Advantages of Exosome Treatment

Exosome treatments have several advantages over the cell-based treatments. Living cell transplantation may cause more safety concerns than exosome treatment. Application of exosomes resolves toxicity and immunogenicity problems caused by biomaterial treatment, such as nanoparticles [10]. The vesicles can both positively and negatively regulate the immune response. MSC-derived exosomes keep the immune privileged properties of their origins. Patients presenting with intestinal graft-versus-host disease grade IV treated with MSC-derived exosomes undergo a significant amelioration of symptoms [69]. However, glioblastoma-derived exosomes activate an immune response to recognized glioblastoma cells. This advantage may greatly help researchers to develop novel immunotherapies [70]. Additionally, nonviable vesicles, compared with living cell transplantation, present a lower risk for severe complication, such as tumors, emboli formation, or GVHD. Furthermore, exosomes are very stable and can be kept approximately 6 months in vitro at −20 °C without loss of potency [71].

10. “Bench to Bedside”: Still a Long Way to Go

Several challenges prevent the development of exosomes into therapeutically agents. One major challenge is to achieve good manufacturing practices. Current exosomes isolation methods provide only a low exosome yield; for example, 5 × 106 myeloma cells provide only 5–6 µg of exosomes [72]. Second, the method of exosome isolation is still controversial. There are two widely used and presumably accepted purification protocols, which use either repeated ultracentrifugation or ultrafiltration [73]. The drawback of these two protocols is the length of time required. Therefore, to develop a reliable method to isolate exosomes will greatly help future studies.
Another major concern is that the exact function of the genetic information that exosomes carry remains elusive. Thus, profiling exosomal contents is a precondition for clinical application. Currently, exosomal miRNAs are one of the major functional components of exosomes. The exosomes content varies according to different origins. Baglio et al. [74] have profiled the miRNA and tRNA information of bone marrow and adipose MSC. The miRNA expression is not significantly different between the cell types. However, the tRNAs show significant difference, especially for Sox2, POU5F1A/B, and Nanog. This finding indicates that the diverse cargoes in exosomes differ greatly according to the origin of the exosome. Furthermore, tumor-supportive miRNA and other bioactive factors are also found in MSC-derived exosomes [75]. Hence, profiling and understanding the exact function of exosomes is a precondition for clinical usage.
Understanding the distribution of injected exosomes is important to control exosome location related side-effects. Studies showed that most exosomes go to bone and the lung; however, other studies have shown that exosomes may also enter the spleen, liver and kidney within the first 30 min after injection. Therefore, a clear investigation of distribution, dosage and clearance will be the foundation for assessing exosome safety [76].

11. Conclusions

In this report, we reviewed recent studies exploring the application of exosomes to regulate osteogenesis and angiogenesis. Although much preliminary data indicated that exosomes stimulate both osteogenesis and angiogenesis, the exact mechanism remains elusive. Before problems can be realized, reliable methods to identify and purify exosomes must first be developed. In addition, a better understanding of the roles that exosomes play in regulating osteogenesis and angiogenesis is also needed. Finally, which cell type- or tissue-derived exosome is the most potent regulator remains to be determined.

Acknowledgments

This work was funded by the National Natural Science Foundation of China (81272003, Changqing Zhang).

Author Contributions

Yunhao Qin designed and wrote this manuscript. Ruixin Sun helped in designing and writing. Chuanlong Wu and Lian Wang helped in the figure design. Changqing Zhang gave basic advice and suggestions. All authors reviewed the manuscript.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Van Griensven, M. Preclinical testing of drug delivery systems to bone. Adv. Drug Deliv. Rev. 2015, 94, 151–164. [Google Scholar] [CrossRef] [PubMed]
  2. Almubarak, S.; Nethercott, H.; Freeberg, M.; Beaudon, C.; Jha, A.; Jackson, W.; Marcucio, R.; Miclau, T.; Healy, K.; Bahney, C. Tissue engineering strategies for promoting vascularized bone regeneration. Bone 2015, 83, 197–209. [Google Scholar] [CrossRef] [PubMed]
  3. Chehade, M.J.; Bachorski, A. Development of the australian core competencies in musculoskeletal basic and clinical science project—Phase 1. Med. J. Aust. 2008, 189, 162–165. [Google Scholar] [PubMed]
  4. Rodriguez-Merchan, E.C.; Forriol, F. Nonunion: General principles and experimental data. Clin. Orthop. Relat. Res. 2004, 419, 4–12. [Google Scholar] [CrossRef] [PubMed]
  5. Mankin, H.J.; Hornicek, F.J.; Raskin, K.A. Infection in massive bone allografts. Clin. Orthop. Relat. Res. 2005, 432, 210–216. [Google Scholar] [CrossRef] [PubMed]
  6. Schwartz, C.E.; Martha, J.F.; Kowalski, P.; Wang, D.A.; Bode, R.; Li, L.; Kim, D.H. Prospective evaluation of chronic pain associated with posterior autologous iliac crest bone graft harvest and its effect on postoperative outcome. Health Qual. Life Outcomes 2009, 7, 49. [Google Scholar] [CrossRef] [PubMed]
  7. Sorger, J.I.; Hornicek, F.J.; Zavatta, M.; Menzner, J.P.; Gebhardt, M.C.; Tomford, W.W.; Mankin, H.J. Allograft fractures revisited. Clin. Orthop. Relat. Res. 2001, 382, 66–74. [Google Scholar] [CrossRef] [PubMed]
  8. Dinopoulos, H.; Dimitriou, R.; Giannoudis, P.V. Bone graft substitutes: What are the options? Surgeon 2012, 10, 230–239. [Google Scholar] [CrossRef] [PubMed]
  9. Stafford, P.R.; Norris, B.L. Reamer-irrigator-aspirator bone graft and bi masquelet technique for segmental bone defect nonunions: A review of 25 cases. Injury 2010, 41, S72–S77. [Google Scholar] [CrossRef]
  10. Fleury, A.; Martinez, M.C.; Le Lay, S. Extracellular vesicles as therapeutic tools in cardiovascular diseases. Front. Immunol. 2014, 5, 370. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  11. Mathivanan, S.; Ji, H.; Simpson, R.J. Exosomes: Extracellular organelles important in intercellular communication. J. Proteom. 2010, 73, 1907–1920. [Google Scholar] [CrossRef] [PubMed]
  12. Hoshino, A.; Costa-Silva, B.; Shen, T.L.; Rodrigues, G.; Hashimoto, A.; Tesic Mark, M.; Molina, H.; Kohsaka, S.; di Giannatale, A.; Ceder, S.; et al. Tumour exosome integrins determine organotropic metastasis. Nature 2015, 527, 329–335. [Google Scholar] [CrossRef] [PubMed]
  13. Trajkovic, K.; Hsu, C.; Chiantia, S.; Rajendran, L.; Wenzel, D.; Wieland, F.; Schwille, P.; Brugger, B.; Simons, M. Ceramide triggers budding of exosome vesicles into multivesicular endosomes. Science 2008, 319, 1244–1247. [Google Scholar] [CrossRef] [PubMed]
  14. Bradley, E.W.; Carpio, L.R.; van Wijnen, A.J.; McGee-Lawrence, M.E.; Westendorf, J.J. Histone deacetylases in bone development and skeletal disorders. Physiol. Rev. 2015, 95, 1359–1381. [Google Scholar] [CrossRef] [PubMed]
  15. Ornitz, D.M.; Marie, P.J. Fgf signaling pathways in endochondral and intramembranous bone development and human genetic disease. Genes Dev. 2002, 16, 1446–1465. [Google Scholar] [CrossRef] [PubMed]
  16. Kronenberg, H.M. Developmental regulation of the growth plate. Nature 2003, 423, 332–336. [Google Scholar] [CrossRef] [PubMed]
  17. Grellier, M.; Bordenave, L.; Amedee, J. Cell-to-cell communication between osteogenic and endothelial lineages: Implications for tissue engineering. Trends Biotechnol. 2009, 27, 562–571. [Google Scholar] [CrossRef] [PubMed]
  18. Gerber, H.P.; Vu, T.H.; Ryan, A.M.; Kowalski, J.; Werb, Z.; Ferrara, N. VEGF couples hypertrophic cartilage remodeling, ossification and angiogenesis during endochondral bone formation. Nat. Med. 1999, 5, 623–628. [Google Scholar] [PubMed]
  19. Hilton, M.J.; Tu, X.; Wu, X.; Bai, S.; Zhao, H.; Kobayashi, T.; Kronenberg, H.M.; Teitelbaum, S.L.; Ross, F.P.; Kopan, R.; et al. Notch signaling maintains bone marrow mesenchymal progenitors by suppressing osteoblast differentiation. Nat. Med. 2008, 14, 306–314. [Google Scholar] [CrossRef] [PubMed]
  20. Ramasamy, S.K.; Kusumbe, A.P.; Wang, L.; Adams, R.H. Endothelial notch activity promotes angiogenesis and osteogenesis in bone. Nature 2014, 507, 376–380. [Google Scholar] [CrossRef] [PubMed]
  21. Trams, E.G.; Lauter, C.J.; Salem, N., Jr.; Heine, U. Exfoliation of membrane ecto-enzymes in the form of micro-vesicles. Biochim. Biophys. Acta 1981, 645, 63–70. [Google Scholar] [CrossRef]
  22. Van Niel, G.; Porto-Carreiro, I.; Simoes, S.; Raposo, G. Exosomes: A common pathway for a specialized function. J. Biochem. 2006, 140, 13–21. [Google Scholar] [CrossRef] [PubMed]
  23. Harding, C.; Heuser, J.; Stahl, P. Receptor-mediated endocytosis of transferrin and recycling of the transferrin receptor in rat reticulocytes. J. Cell Biol. 1983, 97, 329–339. [Google Scholar] [CrossRef] [PubMed]
  24. Pan, B.T.; Johnstone, R.M. Fate of the transferrin receptor during maturation of sheep reticulocytes in vitro: Selective externalization of the receptor. Cell 1983, 33, 967–978. [Google Scholar] [CrossRef]
  25. Thery, C.; Regnault, A.; Garin, J.; Wolfers, J.; Zitvogel, L.; Ricciardi-Castagnoli, P.; Raposo, G.; Amigorena, S. Molecular characterization of dendritic cell-derived exosomes. Selective accumulation of the heat shock protein hsc73. J. Cell Biol. 1999, 147, 599–610. [Google Scholar] [CrossRef] [PubMed]
  26. Raposo, G.; Nijman, H.W.; Stoorvogel, W.; Liejendekker, R.; Harding, C.V.; Melief, C.J.; Geuze, H.J. B lymphocytes secrete antigen-presenting vesicles. J. Exp. Med. 1996, 183, 1161–1172. [Google Scholar] [CrossRef] [PubMed]
  27. Blanchard, N.; Lankar, D.; Faure, F.; Regnault, A.; Dumont, C.; Raposo, G.; Hivroz, C. TCR activation of human t cells induces the production of exosomes bearing the TCR/CD3/ζ complex. J. Immunol. 2002, 168, 3235–3241. [Google Scholar] [CrossRef] [PubMed]
  28. Raposo, G.; Tenza, D.; Mecheri, S.; Peronet, R.; Bonnerot, C.; Desaymard, C. Accumulation of major histocompatibility complex class II molecules in mast cell secretory granules and their release upon degranulation. Mol. Biol. Cell 1997, 8, 2631–2645. [Google Scholar] [CrossRef] [PubMed]
  29. Van Niel, G.; Raposo, G.; Candalh, C.; Boussac, M.; Hershberg, R.; Cerf-Bensussan, N.; Heyman, M. Intestinal epithelial cells secrete exosome-like vesicles. Gastroenterology 2001, 121, 337–349. [Google Scholar] [CrossRef] [PubMed]
  30. Mears, R.; Craven, R.A.; Hanrahan, S.; Totty, N.; Upton, C.; Young, S.L.; Patel, P.; Selby, P.J.; Banks, R.E. Proteomic analysis of melanoma-derived exosomes by two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. Proteomics 2004, 4, 4019–4031. [Google Scholar] [CrossRef] [PubMed]
  31. Valadi, H.; Ekstrom, K.; Bossios, A.; Sjostrand, M.; Lee, J.J.; Lotvall, J.O. Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat. Cell Biol. 2007, 9, 654–659. [Google Scholar] [CrossRef] [PubMed]
  32. Taylor, D.D.; Gercel-Taylor, C. Microrna signatures of tumor-derived exosomes as diagnostic biomarkers of ovarian cancer. Gynecol. Oncol. 2008, 110, 13–21. [Google Scholar] [CrossRef] [PubMed]
  33. Al-Nedawi, K.; Meehan, B.; Micallef, J.; Lhotak, V.; May, L.; Guha, A.; Rak, J. Intercellular transfer of the oncogenic receptor EGFRvIII by microvesicles derived from tumour cells. Nat. Cell Biol. 2008, 10, 619–624. [Google Scholar] [CrossRef] [PubMed]
  34. Luketic, L.; Delanghe, J.; Sobol, P.T.; Yang, P.; Frotten, E.; Mossman, K.L.; Gauldie, J.; Bramson, J.; Wan, Y. Antigen presentation by exosomes released from peptide-pulsed dendritic cells is not suppressed by the presence of active CTL. J. Immunol. 2007, 179, 5024–5032. [Google Scholar] [CrossRef] [PubMed]
  35. Balaj, L.; Lessard, R.; Dai, L.; Cho, Y.J.; Pomeroy, S.L.; Breakefield, X.O.; Skog, J. Tumour microvesicles contain retrotransposon elements and amplified oncogene sequences. Nat. Commun. 2011, 2, 180. [Google Scholar] [CrossRef] [PubMed]
  36. Webber, J.P.; Spary, L.K.; Sanders, A.J.; Chowdhury, R.; Jiang, W.G.; Steadman, R.; Wymant, J.; Jones, A.T.; Kynaston, H.; Mason, M.D.; et al. Differentiation of tumour-promoting stromal myofibroblasts by cancer exosomes. Oncogene 2015, 34, 290–302. [Google Scholar] [CrossRef] [PubMed]
  37. Yamaguchi, T.; Izumi, Y.; Nakamura, Y.; Yamazaki, T.; Shiota, M.; Sano, S.; Tanaka, M.; Osada-Oka, M.; Shimada, K.; Miura, K.; et al. Repeated remote ischemic conditioning attenuates left ventricular remodeling via exosome-mediated intercellular communication on chronic heart failure after myocardial infarction. Int. J. Cardiol. 2015, 178, 239–246. [Google Scholar] [CrossRef] [PubMed]
  38. Denzer, K.; van Eijk, M.; Kleijmeer, M.J.; Jakobson, E.; de Groot, C.; Geuze, H.J. Follicular dendritic cells carry MHC class II-expressing microvesicles at their surface. J. Immunol. 2000, 165, 1259–1265. [Google Scholar] [CrossRef] [PubMed]
  39. Clayton, A.; Turkes, A.; Dewitt, S.; Steadman, R.; Mason, M.D.; Hallett, M.B. Adhesion and signaling by B cell-derived exosomes: The role of integrins. FASEB J. 2004, 18, 977–979. [Google Scholar] [CrossRef] [PubMed]
  40. Morelli, A.E.; Larregina, A.T.; Shufesky, W.J.; Sullivan, M.L.; Stolz, D.B.; Papworth, G.D.; Zahorchak, A.F.; Logar, A.J.; Wang, Z.; Watkins, S.C.; et al. Endocytosis, intracellular sorting, and processing of exosomes by dendritic cells. Blood 2004, 104, 3257–3266. [Google Scholar] [CrossRef] [PubMed]
  41. Koppers-Lalic, D.; Hogenboom, M.M.; Middeldorp, J.M.; Pegtel, D.M. Virus-modified exosomes for targeted rna delivery; a new approach in nanomedicine. Adv. Drug Deliv. Rev. 2013, 65, 348–356. [Google Scholar] [CrossRef] [PubMed]
  42. Simpson, R.J.; Jensen, S.S.; Lim, J.W. Proteomic profiling of exosomes: Current perspectives. Proteomics 2008, 8, 4083–4099. [Google Scholar] [CrossRef] [PubMed]
  43. Van Dommelen, S.M.; Vader, P.; Lakhal, S.; Kooijmans, S.A.; van Solinge, W.W.; Wood, M.J.; Schiffelers, R.M. Microvesicles and exosomes: Opportunities for cell-derived membrane vesicles in drug delivery. J. Control. Release 2012, 161, 635–644. [Google Scholar] [CrossRef] [PubMed]
  44. Gibbings, D.; Voinnet, O. Control of RNA silencing and localization by endolysosomes. Trends Cell Biol. 2010, 20, 491–501. [Google Scholar] [CrossRef] [PubMed]
  45. Pegtel, D.M.; Cosmopoulos, K.; Thorley-Lawson, D.A.; van Eijndhoven, M.A.; Hopmans, E.S.; Lindenberg, J.L.; de Gruijl, T.D.; Wurdinger, T.; Middeldorp, J.M. Functional delivery of viral mirnas via exosomes. Proc. Natl. Acad. Sci. USA 2010, 107, 6328–6333. [Google Scholar] [CrossRef] [PubMed]
  46. Lee, C.; Mitsialis, S.A.; Aslam, M.; Vitali, S.H.; Vergadi, E.; Konstantinou, G.; Sdrimas, K.; Fernandez-Gonzalez, A.; Kourembanas, S. Exosomes mediate the cytoprotective action of mesenchymal stromal cells on hypoxia-induced pulmonary hypertension. Circulation 2012, 126, 2601–2611. [Google Scholar] [CrossRef] [PubMed]
  47. Zhou, Y.; Xu, H.; Xu, W.; Wang, B.; Wu, H.; Tao, Y.; Zhang, B.; Wang, M.; Mao, F.; Yan, Y.; et al. Exosomes released by human umbilical cord mesenchymal stem cells protect against cisplatin-induced renal oxidative stress and apoptosis in vivo and in vitro. Stem Cell Res. Ther. 2013, 4, 34. [Google Scholar] [CrossRef] [PubMed]
  48. Alvarez-Erviti, L.; Seow, Y.; Yin, H.; Betts, C.; Lakhal, S.; Wood, M.J. Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes. Nat. Biotechnol. 2011, 29, 341–345. [Google Scholar] [CrossRef] [PubMed]
  49. Basu, J.; Ludlow, J.W. Exosomes for repair, regeneration and rejuvenation. Expert Opin. Biol. Ther. 2016, 16, 489–506. [Google Scholar] [CrossRef] [PubMed]
  50. Narayanan, R.; Huang, C.C.; Ravindran, S. Hijacking the cellular mail: Exosome mediated differentiation of mesenchymal stem cells. Stem Cells Int. 2016, 2016, 3808674. [Google Scholar] [CrossRef] [PubMed]
  51. Xu, J.F.; Yang, G.H.; Pan, X.H.; Zhang, S.J.; Zhao, C.; Qiu, B.S.; Gu, H.F.; Hong, J.F.; Cao, L.; Chen, Y.; et al. Altered microrna expression profile in exosomes during osteogenic differentiation of human bone marrow-derived mesenchymal stem cells. PLoS ONE 2014, 9, e114627. [Google Scholar] [CrossRef] [PubMed]
  52. Cui, Y.; Luan, J.; Li, H.; Zhou, X.; Han, J. Exosomes derived from mineralizing osteoblasts promote ST2 cell osteogenic differentiation by alteration of microrna expression. FEBS Lett. 2016, 590, 185–192. [Google Scholar] [CrossRef] [PubMed]
  53. Ge, M.; Ke, R.; Cai, T.; Yang, J.; Mu, X. Identification and proteomic analysis of osteoblast-derived exosomes. Biochem. Biophys. Res. Commun. 2015, 467, 27–32. [Google Scholar] [CrossRef] [PubMed]
  54. Wang, Z.; Ding, L.; Zheng, X.L.; Wang, H.X.; Yan, H.M. DC-derived exosomes induce osteogenic differentiation of mesenchymal stem cells. Zhongguo Shi Yan Xue Ye Xue Za Zhi 2014, 22, 600–604. [Google Scholar] [PubMed]
  55. Ekstrom, K.; Omar, O.; Graneli, C.; Wang, X.; Vazirisani, F.; Thomsen, P. Monocyte exosomes stimulate the osteogenic gene expression of mesenchymal stem cells. PLoS ONE 2013, 8, e75227. [Google Scholar] [CrossRef] [PubMed]
  56. Golub, E.E. Biomineralization and matrix vesicles in biology and pathology. Semin. Immunopathol. 2011, 33, 409–417. [Google Scholar] [CrossRef] [PubMed]
  57. Inder, K.L.; Ruelcke, J.E.; Petelin, L.; Moon, H.; Choi, E.; Rae, J.; Blumenthal, A.; Hutmacher, D.; Saunders, N.A.; Stow, J.L.; et al. Cavin-1/PTRF alters prostate cancer cell-derived extracellular vesicle content and internalization to attenuate extracellular vesicle-mediated osteoclastogenesis and osteoblast proliferation. J. Extracell. Vesicles 2014, 3, 23784. [Google Scholar] [CrossRef] [PubMed]
  58. Kawakubo, A.; Matsunaga, T.; Ishizaki, H.; Yamada, S.; Hayashi, Y. Zinc as an essential trace element in the acceleration of matrix vesicles-mediated mineral deposition. Microsc. Res. Tech. 2011, 74, 1161–1165. [Google Scholar] [CrossRef] [PubMed]
  59. Qin, Y.; Wang, L.; Gao, Z.; Chen, G.; Zhang, C. Bone marrow stromal/stem cell-derived extracellular vesicles regulate osteoblast activity and differentiation in vitro and promote bone regeneration in vivo. Sci. Rep. 2016, 6, 21961. [Google Scholar] [CrossRef] [PubMed]
  60. Raimondi, L.; de Luca, A.; Amodio, N.; Manno, M.; Raccosta, S.; Taverna, S.; Bellavia, D.; Naselli, F.; Fontana, S.; Schillaci, O.; et al. Involvement of multiple myeloma cell-derived exosomes in osteoclast differentiation. Oncotarget 2015, 6, 13772–13789. [Google Scholar] [CrossRef] [PubMed]
  61. Solberg, L.B.; Stang, E.; Brorson, S.H.; Andersson, G.; Reinholt, F.P. Tartrate-resistant acid phosphatase (TRAP) co-localizes with receptor activator of NF-KB ligand (RANKL) and osteoprotegerin (OPG) in lysosomal-associated membrane protein 1 (LAMP1)-positive vesicles in rat osteoblasts and osteocytes. Histochem. Cell Biol. 2015, 143, 195–207. [Google Scholar] [CrossRef] [PubMed]
  62. Huynh, N.; VonMoss, L.; Smith, D.; Rahman, I.; Felemban, M.F.; Zuo, J.; Rody, W.J., Jr.; McHugh, K.P.; Holliday, L.S. Characterization of regulatory extracellular vesicles from osteoclasts. J. Dent. Res. 2016. [Google Scholar] [CrossRef] [PubMed]
  63. Salomon, C.; Ryan, J.; Sobrevia, L.; Kobayashi, M.; Ashman, K.; Mitchell, M.; Rice, G.E. Exosomal signaling during hypoxia mediates microvascular endothelial cell migration and vasculogenesis. PLoS ONE 2013, 8, e68451. [Google Scholar] [CrossRef] [PubMed]
  64. Janiszewski, M.; Do Carmo, A.O.; Pedro, M.A.; Silva, E.; Knobel, E.; Laurindo, F.R. Platelet-derived exosomes of septic individuals possess proapoptotic NAD(P)H oxidase activity: A novel vascular redox pathway. Crit. Care Med. 2004, 32, 818–825. [Google Scholar] [CrossRef] [PubMed]
  65. Vrijsen, K.R.; Sluijter, J.P.; Schuchardt, M.W.; van Balkom, B.W.; Noort, W.A.; Chamuleau, S.A.; Doevendans, P.A. Cardiomyocyte progenitor cell-derived exosomes stimulate migration of endothelial cells. J. Cell. Mol. Med. 2010, 14, 1064–1070. [Google Scholar] [CrossRef] [PubMed]
  66. Sahoo, S.; Klychko, E.; Thorne, T.; Misener, S.; Schultz, K.M.; Millay, M.; Ito, A.; Liu, T.; Kamide, C.; Agrawal, H.; et al. Exosomes from human CD34(+) stem cells mediate their proangiogenic paracrine activity. Circ. Res. 2011, 109, 724–728. [Google Scholar] [CrossRef] [PubMed]
  67. Bian, S.; Zhang, L.; Duan, L.; Wang, X.; Min, Y.; Yu, H. Extracellular vesicles derived from human bone marrow mesenchymal stem cells promote angiogenesis in a rat myocardial infarction model. J. Mol. Med. 2014, 92, 387–397. [Google Scholar] [CrossRef] [PubMed]
  68. Zhang, H.C.; Liu, X.B.; Huang, S.; Bi, X.Y.; Wang, H.X.; Xie, L.X.; Wang, Y.Q.; Cao, X.F.; Lv, J.; Xiao, F.J.; et al. Microvesicles derived from human umbilical cord mesenchymal stem cells stimulated by hypoxia promote angiogenesis both in vitro and in vivo. Stem Cells Dev. 2012, 21, 3289–3297. [Google Scholar] [CrossRef] [PubMed]
  69. Kordelas, L.; Rebmann, V.; Ludwig, A.K.; Radtke, S.; Ruesing, J.; Doeppner, T.R.; Epple, M.; Horn, P.A.; Beelen, D.W.; Giebel, B. Msc-derived exosomes: A novel tool to treat therapy-refractory graft-versus-host disease. Leukemia 2014, 28, 970–973. [Google Scholar] [CrossRef] [PubMed]
  70. Harshyne, L.A.; Nasca, B.J.; Kenyon, L.C.; Andrews, D.W.; Hooper, D.C. Serum exosomes and cytokines promote a T-helper cell type 2 environment in the peripheral blood of glioblastoma patients. Neuro Oncol. 2016, 18, 206–215. [Google Scholar] [CrossRef] [PubMed]
  71. Yu, B.; Zhang, X.; Li, X. Exosomes derived from mesenchymal stem cells. Int. J. Mol. Sci. 2014, 15, 4142–4157. [Google Scholar] [CrossRef] [PubMed]
  72. Peinado, H.; Aleckovic, M.; Lavotshkin, S.; Matei, I.; Costa-Silva, B.; Moreno-Bueno, G.; Hergueta-Redondo, M.; Williams, C.; Garcia-Santos, G.; Ghajar, C.; et al. Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through met. Nat. Med. 2012, 18, 883–891. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  73. Lobb, R.J.; Becker, M.; Wen, S.W.; Wong, C.S.; Wiegmans, A.P.; Leimgruber, A.; Moller, A. Optimized exosome isolation protocol for cell culture supernatant and human plasma. J. Extracell. Vesicles 2015, 4, 27031. [Google Scholar] [CrossRef] [PubMed]
  74. Baglio, S.R.; Rooijers, K.; Koppers-Lalic, D.; Verweij, F.J.; Perez Lanzon, M.; Zini, N.; Naaijkens, B.; Perut, F.; Niessen, H.W.; Baldini, N.; et al. Human bone marrow- and adipose-mesenchymal stem cells secrete exosomes enriched in distinctive mirna and trna species. Stem Cell Res. Ther. 2015, 6, 127. [Google Scholar] [CrossRef] [PubMed]
  75. Vallabhaneni, K.C.; Penfornis, P.; Dhule, S.; Guillonneau, F.; Adams, K.V.; Mo, Y.Y.; Xu, R.; Liu, Y.; Watabe, K.; Vemuri, M.C.; et al. Extracellular vesicles from bone marrow mesenchymal stem/stromal cells transport tumor regulatory microRNA, proteins, and metabolites. Oncotarget 2015, 6, 4953–4967. [Google Scholar] [CrossRef] [PubMed]
  76. Lai, C.P.; Mardini, O.; Ericsson, M.; Prabhakar, S.; Maguire, C.A.; Chen, J.W.; Tannous, B.A.; Breakefield, X.O. Dynamic biodistribution of extracellular vesicles in vivo using a multimodal imaging reporter. ACS Nano 2014, 8, 483–494. [Google Scholar] [CrossRef] [PubMed]
  77. Hu, G.W.; Li, Q.; Niu, X.; Hu, B.; Liu, J.; Zhou, S.M.; Guo, S.C.; Lang, H.L.; Zhang, C.Q.; Wang, Y.; et al. Exosomes secreted by human-induced pluripotent stem cell-derived mesenchymal stem cells attenuate limb ischemia by promoting angiogenesis in mice. Stem Cell Res. Ther. 2015, 6, 10. [Google Scholar] [CrossRef] [PubMed]
  78. Taverna, S.; Flugy, A.; Saieva, L.; Kohn, E.C.; Santoro, A.; Meraviglia, S.; de Leo, G.; Alessandro, R. Role of exosomes released by chronic myelogenous leukemia cells in angiogenesis. Int. J. Cancer 2012, 130, 2033–2043. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  79. Bergers, G.; Benjamin, L.E. Tumorigenesis and the angiogenic switch. Nat. Rev. Cancer 2003, 3, 401–410. [Google Scholar] [CrossRef] [PubMed]
  80. Umezu, T.; Ohyashiki, K.; Kuroda, M.; Ohyashiki, J.H. Leukemia cell to endothelial cell communication via exosomal mirnas. Oncogene 2013, 32, 2747–2755. [Google Scholar] [CrossRef] [PubMed]
  81. Lopatina, T.; Bruno, S.; Tetta, C.; Kalinina, N.; Porta, M.; Camussi, G. Platelet-derived growth factor regulates the secretion of extracellular vesicles by adipose mesenchymal stem cells and enhances their angiogenic potential. Cell Commun. Signal. 2014, 12, 26. [Google Scholar] [CrossRef] [PubMed]
Ijms 17 00712 g001 1024
Figure 1. Exosome biogenesis: exosomes are generated in two distinct ways: the endocytic pathway and the biosynthetic pathway. The endocytic pathway begins by receiving extrinsic or intrinsic signals from the local milieu. Then, the plasma membrane begins to invaginate, and the early endosome subsequently forms. The early endosome becomes the late endosome under the regulation of multiple cell signaling pathways. The Golgi Apparatus and the endoplasmic reticulum also participate in exosome secretion. The multivesicle body resulting from the late endosome fuses with the plasma membrane and releases the exosome, or undergoes degradation.
Figure 1. Exosome biogenesis: exosomes are generated in two distinct ways: the endocytic pathway and the biosynthetic pathway. The endocytic pathway begins by receiving extrinsic or intrinsic signals from the local milieu. Then, the plasma membrane begins to invaginate, and the early endosome subsequently forms. The early endosome becomes the late endosome under the regulation of multiple cell signaling pathways. The Golgi Apparatus and the endoplasmic reticulum also participate in exosome secretion. The multivesicle body resulting from the late endosome fuses with the plasma membrane and releases the exosome, or undergoes degradation.
Ijms 17 00712 g001
Table
Table 1. Exosome, microvesicle, apoptotic body: major similarities and differences.
Table 1. Exosome, microvesicle, apoptotic body: major similarities and differences.
CharacteristicExosomeMicrovesicleApoptotic Body
Size50–120 nm100–1000 nm50–500 nm
MorphologyCup-shapedHeterogeneousHeterogeneous
Protein MarkerAlix, Tsg101, CD63, CD9Selectins, integrins, CD40Histones
OriginMultivesicular BodyPlasma MembraneProgrammed cell death
Mechanism of dischargeExocytosis of MVBsBudding from plasma membraneCell shrinkage and death
CompositionProtein, miRNA, mRNAProtein, miRNA, mRNAProtein, DNA, miRNA, mRNA
Table
Table 2. Reported roles of exosomes in osteogenesis and angiogenesis.
Table 2. Reported roles of exosomes in osteogenesis and angiogenesis.
Origin of ExosomesContent ProfileIn Vitro EffectIn Vivo Effect
MSCs [50]Not mentionedInduce osteogenesis differentiation in naive stem cellsNo in vivo data
Mineralizing osteoblasts [52]Axin1 inhibitorPromote osteoblastic differentiation by activating Wnt signalingNo in vivo data
Osteoblasts [53]Tumor susceptibility gene 101, flotillin 1 and 1069 other proteinsPromote osteoblastic differentiation by activating eukaryotic initiation factor 2No in vivo data
Monocytes [55]Small RNAs are enriched in exosomesRunt-Related Transcript Factor 2, Osteocalcin and Bone Morphogenetic Protein 2 were Up-regulated in bone mesenchymal stem cellsNo in vivo data
Prostate cancer cells [57]miR-148a, miR-125aIncreased osteoblast proliferationMost PKH2 labeled exosomes go to lung and bone marrow in 24 h. (liver, spleen, kidney, heart, thymus, brain, prostate)
Matrix [58]Not mentionedIncrease Alkaline Phosphatase activity of osteoblast; Increase mineral depositionNo in vivo data
Bone MSCs [59]miR-196aIncreased osteoblast activity Stimulate bone growth in calvarial bone defect models
Myeloma cells [60]Not mentionedInduce pre-osteoclast maturation and migrationNo in vivo data
Promote osteoclast differentiation
Osteoclasts [62]RANKInduce osteoclast differentiationNo in vivo data
Placental MSCs [63]157 proteins enriched.Increase endothelial cell migration, tube formationNo in vivo data
Platelets [64]P22phox and gp91phox subunit of NADPH oxidaseStimulate mRNA expression for angiogenic factors: Matrix metallopeptidase 9, vascular endothelial growth factor, interleukin-8, hepatocyte growth factor in endothelial cellsNo in vivo data
Myocardial progenitor cells [65]Metalloproteinases, extracellular matrix metalloproteinase inducerIncrease endothelial cell migrationNo in vivo data
Bone marrow derived-stem cells [66]miR-126, miR-139Increase endothelial cell viability, proliferation and tube formationNo in vivo data
MSCs [67]Not mentionedIncrease endothelial cells proliferation, migration and tube formationReduce myocardial ischemic/reperfusion injury; Improve angiogenesis in ischemic heart
Human umbilical cord derived MSCs [68]Not mentionedIncrease endothelial cells proliferation, network formation. Significantly increased blood flow in ischemic modelPromote blood perfusion and attenuate hind-limb ischemia
Human induced pluripotent stem cell derived MSCs [77]Not mentionedIncrease endothelial cell migration, proliferation, and tube formationPromote blood perfusion and attenuate severe hind-limb ischemia
Chronic myeloid leukemia cells [78]Not mentionedIncrease endothelial cell migration and tube formationPromote matrigel induced tube formation in nude mice
Myelogenous leukemia [79] Increase endothelial cell motility, ingrowth and vascularization
Leukemia cells [80]miR-17-92 clusterIncrease endothelial cell migration, proliferation and vessel formationNo in vivo data
Adipose MSC [81]Artemin, Axl, Milk Fat Globule-EGF Factor-8, Oncostatin M, Stem Cell Factor, and thrombopoietin are enriched.Increase vessel-like formationPromote vessel formation in subcutaneous gel

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Qin, Y.; Sun, R.; Wu, C.; Wang, L.; Zhang, C. Exosome: A Novel Approach to Stimulate Bone Regeneration through Regulation of Osteogenesis and Angiogenesis. Int. J. Mol. Sci. 2016, 17, 712. https://doi.org/10.3390/ijms17050712

AMA Style

Qin Y, Sun R, Wu C, Wang L, Zhang C. Exosome: A Novel Approach to Stimulate Bone Regeneration through Regulation of Osteogenesis and Angiogenesis. International Journal of Molecular Sciences. 2016; 17(5):712. https://doi.org/10.3390/ijms17050712

Chicago/Turabian Style

Qin, Yunhao, Ruixin Sun, Chuanlong Wu, Lian Wang, and Changqing Zhang. 2016. "Exosome: A Novel Approach to Stimulate Bone Regeneration through Regulation of Osteogenesis and Angiogenesis" International Journal of Molecular Sciences 17, no. 5: 712. https://doi.org/10.3390/ijms17050712

APA Style

Qin, Y., Sun, R., Wu, C., Wang, L., & Zhang, C. (2016). Exosome: A Novel Approach to Stimulate Bone Regeneration through Regulation of Osteogenesis and Angiogenesis. International Journal of Molecular Sciences, 17(5), 712. https://doi.org/10.3390/ijms17050712

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