A GPAT1 Mutation in Arabidopsis Enhances Plant Height but Impairs Seed Oil Biosynthesis
Abstract
:1. Introduction
2. Results
2.1. Expression Pattern of the Arabidopsis GPAT1 Gene
2.2. Knockout of GPAT1 Decreases TAG Content and Alters FA Composition in Seeds
2.3. Knockout of GPAT1 Enhances Plant Height but Decreases Seed Yield
2.4. Knockout of GPAT1 Alters Cell Morphology of the Stem
2.5. Transcriptome Differences between WT and gpat1 Mutant Plants
2.6. Validation of DEGs Using qRT-PCR
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. β-Glucuronidase (GUS) Staining Assay and Light Microscopy
4.3. qRT-PCR and Reverse Transcription PCR
4.4. Mutant Creation, Identification and Complementation
4.5. Cytological Observation
4.6. Seed TAG and Fatty Acid (FA) Analysis
4.7. RNA-Sequencing and Data Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
GPAT | glycerol-3-phosphate acyltransferase |
GA | gibberellin |
MEP | methylerythritol phosphate |
GGPP | geranylgeranyl diphosphate |
CPS | copalyl diphosphate synthase |
KS | ent-kaurene synthase |
KO | ent-kaurene oxidase |
KAO | ent-kaurenoic acid oxidase |
2-OGD | 2-oxoglutarate-dependent dioxygenase |
GID1 | gibberellin insensitive dwarf 1 |
XTH | xyloglucan endotransglucosylase/hydrolase |
PME | pectin methylesterase |
PMEI | pectin methylesterase inhibitor |
EXP | expansin |
TAG | triacylglycerol |
FA | fatty acid |
GO | gene ontology |
KEGG | Kyoto encyclopedia of genes and genomes |
DEGs | differentially expressed genes |
TPM | Transcripts Per Million |
NPF | nitrate transporter 1/peptide transporter family |
SWEET | sugar will eventually be exported transporter |
RGP | reversibly glycosylated protein |
XYN | endo-1,4-b-xylanase |
CSL | cellulose synthase like proteins |
EXT | extensin |
GUS | β-glucuronidase |
BHT | 2,6-Di-tert-butyl-4-methylpheno |
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Bai, Y.; Shen, Y.; Zhang, Z.; Jia, Q.; Xu, M.; Zhang, T.; Fang, H.; Yu, X.; Li, L.; Liu, D.; et al. A GPAT1 Mutation in Arabidopsis Enhances Plant Height but Impairs Seed Oil Biosynthesis. Int. J. Mol. Sci. 2021, 22, 785. https://doi.org/10.3390/ijms22020785
Bai Y, Shen Y, Zhang Z, Jia Q, Xu M, Zhang T, Fang H, Yu X, Li L, Liu D, et al. A GPAT1 Mutation in Arabidopsis Enhances Plant Height but Impairs Seed Oil Biosynthesis. International Journal of Molecular Sciences. 2021; 22(2):785. https://doi.org/10.3390/ijms22020785
Chicago/Turabian StyleBai, Yang, Yue Shen, Zhiqiang Zhang, Qianru Jia, Mengyuan Xu, Ting Zhang, Hailing Fang, Xu Yu, Li Li, Dongmei Liu, and et al. 2021. "A GPAT1 Mutation in Arabidopsis Enhances Plant Height but Impairs Seed Oil Biosynthesis" International Journal of Molecular Sciences 22, no. 2: 785. https://doi.org/10.3390/ijms22020785
APA StyleBai, Y., Shen, Y., Zhang, Z., Jia, Q., Xu, M., Zhang, T., Fang, H., Yu, X., Li, L., Liu, D., Qi, X., Chen, Z., Wu, S., Zhang, Q., & Liang, C. (2021). A GPAT1 Mutation in Arabidopsis Enhances Plant Height but Impairs Seed Oil Biosynthesis. International Journal of Molecular Sciences, 22(2), 785. https://doi.org/10.3390/ijms22020785