Next Article in Journal
Special Issue “Centenarians—A Model to Study the Molecular Basis of Lifespan and Healthspan”
Next Article in Special Issue
Comparative Genomics: Insights on the Pathogenicity and Lifestyle of Rhizoctonia solani
Previous Article in Journal
Assessment of Epinephrine and Norepinephrine in Gastric Carcinoma
Previous Article in Special Issue
Two 20-Residue-Long Peptides Derived from Plasmodium vivax Merozoite Surface Protein 10 EGF-Like Domains Are Involved in Binding to Human Reticulocytes
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 22
Issue 4
10.3390/ijms22042043
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Immune-Mediated Aggravation of the Campylobacter concisus-Induced Epithelial Barrier Dysfunction

by
Praveen Kumar Nattramilarasu
,
Fábia Daniela Lobo de Sá
,
Jörg-Dieter Schulzke
and
Roland Bücker
*
Institute of Clinical Physiology/Nutritional Medicine, Medical Department, Division of Gastroenterology, Infectiology and Rheumatology, Charité—Universitätsmedizin Berlin, 12203 Berlin, Germany
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2021, 22(4), 2043; https://doi.org/10.3390/ijms22042043
Submission received: 30 December 2020 / Revised: 9 February 2021 / Accepted: 15 February 2021 / Published: 19 February 2021
(This article belongs to the Special Issue Host-Pathogen Interaction 2.0)
Browse Figures
Versions Notes

Abstract

:
Campylobacter concisus is a human-pathogenic bacterium of the gastrointestinal tract. This study aimed at the contribution of the mucosal immune system in the context of intestinal epithelial barrier dysfunction induced by C. concisus. As an experimental leaky gut model, we used in vitro co-cultures of colonic epithelial cell monolayers (HT-29/B6-GR/MR) with M1-macrophage-like THP-1 cells on the basal side. Forty-eight hours after C. concisus infection, the decrease in the transepithelial electrical resistance in cell monolayers was more pronounced in co-culture condition and 22 ± 2% (p < 0.001) higher than the monoculture condition without THP-1 cells. Concomitantly, we observed a reduction in the expression of the tight junction proteins occludin and tricellulin. We also detected a profound increase in 4 kDa FITC-dextran permeability in C. concisus-infected cell monolayers only in co-culture conditions. This is explained by loss of tricellulin from tricellular tight junctions (tTJs) after C. concisus infection. As an underlying mechanism, we observed an inflammatory response after C. concisus infection through pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) released from THP-1 cells in the co-culture condition. In conclusion, the activation of subepithelial immune cells exacerbates colonic epithelial barrier dysfunction by C. concisus through tricellulin disruption in tTJs, leading to increased antigen permeability (leaky gut concept).

1. Introduction

Campylobacter concisus is a Gram-negative epsilon-proteobacterium discovered as a pathobiont in the human oral cavity of the patients with severe gingival inflammation or periodontitis [1]. Clinical epidemiological studies detected C. concisus in diarrheal feces of children and immunocompromised patients [2,3]. Most importantly, one clinical study with a large cohort of diarrheal patients discovered that C. concisus induced prolonged diarrhea with less fever, unlike zoonotic diarrheal pathogens Campylobacter coli and Campylobacter jejuni, which cause short-term diarrhea with more often fever and increased C-Reactive Protein (CRP) levels [4]. In our previous study, we reported that C. concisus impaired epithelial sodium channel (ENaC) function via activation of extracellular signal-regulated kinase (ERK) and induced claudin-8-dependent barrier dysfunction, both of which contribute to sodium malabsorption and watery diarrhea [5]. The diarrheal mechanism of C. concisus correlates to the diarrheal pathomechanism of lymphocytic colitis, a subtype of microscopic colitis [6]. In recent conclusions from clinical studies, C. concisus-infected diarrheal patients were found to exhibit a higher risk for developing microscopic colitis [7,8]. Interestingly, in lymphocytic colitis patients, the compromised colonic epithelial barrier function is accompanied by a decrease in the expression of tight junction (TJ) proteins, claudin-4, -5, and -8 [9].
Tight junctions are the essential components of intercellular interfaces in the intestinal epithelia of enterocytes which regulate the barrier function. Among TJ proteins, claudins play a vital role in sealing the paracellular space between the enterocytes (fence function) [10,11]. However, claudins also act as channels for the transport of water and ions in the intestine to regulate paracellular permeability (gate function) [12,13]. In addition to claudins, occludin and tricellulin play a crucial role in regulating macromolecule permeability, and thereby act as a paracellular barrier to the luminal antigens [14,15,16]. The structure of tricellular TJ (tTJ) is unique with three pairs of TJ strands called central sealing elements [17], and its role in regulating the paracellular permeability was first observed in airway epithelia of guinea pigs [18]. Later, tricellulin was identified as an integral component of tTJ in vertebrate epithelia [19]. Surprisingly, tricellulin localization in tTJ was regulated by the bicellular tight junction (bTJ) protein occludin [20].
The mucosal immune system of the gastrointestinal tract is confronted with the antigens or microorganisms that pass the epithelial barrier. The lymphoid cells induce antigen-specific immune responses, whereas macrophages and granulocytes elicit non-specific immune responses [21]. However, in both inflammatory bowel disease (IBD) subtypes; Crohn’s Disease (CD) and ulcerative colitis (UC), the dysregulation of mucosal immune responses leads to an uncontrolled inflammation [22]. The inflammatory microenvironment in the dextran sodium sulfate (DSS)-induced colitis mouse model resulted in the accumulation of Toll-like receptor (TLR) responsive pro-inflammatory macrophages [23,24]. The lipopolysaccharide (LPS) component of the Gram-negative bacterial cell wall, which acts via TLR-4, was ascertained as an important virulence factor that aggravates the intestinal inflammation in IBD patients [25,26]. Furthermore, epithelial barrier dysfunction was ascertained in colonic epithelial cell monolayers in co-culture with M1 macrophages, the barrier dysfunction of which was induced by tumor necrosis factor-α (TNFα)-mediated deregulation of TJ proteins and epithelial apoptosis [27]. Earlier studies reported that C. concisus induces moderate changes in TJ expression and epithelial apoptosis, as a consequence of which intestinal epithelial barrier function is compromised [28,29].
A few clinical reports indicated the increased prevalence of C. concisus in the feces of inflammatory bowel disease (IBD) patients [30,31,32,33]. Hence, it becomes indispensable to investigate the link between intestinal inflammation and C. concisus pathogenesis, especially because increased intestinal colonization of C. concisus in IBD patients might result in exacerbation of inflammation and diarrhea [34,35]. C. concisus infection has indeed been shown to induce activation of immune cells and the release of pro-inflammatory cytokines [28]. However, the relative contribution of subepithelial immune cell activation on the overall intestinal epithelial barrier dysfunction induced by C. concisus, and the mechanisms of the epithelial barrier impairment and the pattern of tight junction (TJ) protein changes remained unexplored. Thus, the primary objective of this study was to elucidate this in more detail. For this purpose, we used an in vitro co-culture model of the colonic epithelial cell (HT-29/B6-GR/MR) monolayers with M1-macrophage-like THP-1 cells on the basal side [36,37], which depicts a cell model for the leaky gut situation as described in earlier studies from our group on E. coli and C. jejuni infections in the human colon [38,39]. As an important objective, we ascertained the TJ changes and apoptotic events induced by C. concisus in our in vitro co-culture model. Furthermore, to elucidate the mechanisms of the functional changes in the colonic epithelial barrier after C. concisus infection, we studied the cytokine release from M1 macrophages in the co-culture setting.

2. Results

2.1. Campylobacter concisus Aggravates the Barrier Disturbance of Intestinal Epithelial Cells in Co-Culture with Immune Cells

The changes in transepithelial electrical resistance (TER) of the cell monolayers were determined in both monoculture (HT-29/B6-GR/MR cell monolayers) and co-culture conditions (M1 macrophage-like THP-1 cells on the basal side of HT-29/B6-GR/MR cell monolayers). A clear decrease in TER of the C. concisus-infected cell monolayers was observed at 24 h post infection (p.i.) when compared to uninfected controls. However, at 24 h p.i., there was no significant difference between the TER of infected cell monolayers in the co-culture and the monoculture condition (Figure 1). At 48 h p.i., a further decrease in TER of the C. concisus-infected cell monolayers was observed in both monoculture and co-culture conditions. Interestingly, we observed a pronounced decrease in TER at 48 h, but not 24 h after C. concisus infection in the co-culture compared to the monoculture condition (Figure 1).

2.2. Protein Expression Changes of Claudins, Occludin and Tricellulin in Colonic Epithelial Cells after C. concisus Infection

To investigate the protein expression of tight junctions (TJs) during the TER decrease in HT-29/B6-GR/MR cells following C. concisus infection, a comprehensive TJ protein analysis of different claudins, occludin and tricellulin was performed in monoculture and co-culture conditions 48 h after C. concisus infection. Among barrier-forming claudins, we found a significant increase in claudin-1 expression and a decrease in claudin-5 expression 48 h after C. concisus infection, when compared to controls in both monoculture and co-culture conditions (Figure 2). The expression of claudin-2, claudin-7, and claudin-8 was unaltered after C. concisus infection in both monoculture and co-culture conditions (Figure 2). However, we found a tendency for an increase in claudin-4 expression in both monoculture and co-culture conditions, but this tendency failed to reach statistical significance (Figure 2). Furthermore, we observed no significant changes in the expression of occludin and tricellulin between the infected cells and controls in the monoculture condition. However, in the co-culture condition, the expression of both occludin and tricellulin decreased 48 h p.i. (Figure 2).

2.3. Subcellular Localization of Occludin and Tricellulin in Colonic Epithelial Cells after Campylobacter concisus Infection

We performed confocal laser-scanning microscopy analysis with Z-stacks of XY-scans from the epithelial cell monolayers, to determine if the delocalization of occludin and tricellulin from the intercellular junctions accompanied the protein expression changes. Forty-eight hours following C. concisus infection, we observed a redistribution of occludin from the bicellular tight junction (bTJ) to subapical intracellular regions in HT-29/B6-GR/MR cell monolayers maintained in co-culture condition, while zonula occludens protein-1 (ZO-1) remained intact in the bTJ. By contrast, at 48 h p.i., the redistribution of occludin from bTJ into subcellular spaces was minimal or almost absent in monoculture condition (Figure 3). In the controls of both monoculture and co-culture cell monolayers, the ZO-1 and occludin signals remained intact in the bTJ with a proper co-localization (Figure 3).
In the control cell monolayers of monoculture and co-culture conditions, tricellulin remained in the tricellular tight junction (tTJ), co-localized with ZO-1 (Figure 4). In the infected monoculture condition at 48 h p.i., redistribution of tricellulin from tTJ into intracellular compartments was rarely observed. However, in the co-culture condition together with M1-macrophage-like THP-1 cells, redistribution of tricellulin off the tTJ into intracellular regions was observed after C. concisus infection (Figure 4). It was also accompanied by focal leaks where ZO-1 was also redistributed off the TJ.

2.4. Epithelial Permeability in Campylobacter concisus-Infected Colonic Epithelial Cell Monolayers

We observed a pronounced drop in TER following C. concisus infection of cell monolayers in co-culture compared to the monoculture condition (Figure 1), representing ion permeability changes. Hence, we performed epithelial cell permeability assays for macromolecules using two fluorescent tracers of different molecular weight, namely fluorescein (332 Da) and FITC-dextran (4 kDa). As expected, we detected an increase in epithelial permeability for fluorescein (332 Da) in cell monolayers after C. concisus infection in both monoculture and co-culture condition (Figure 5). However, the increase in permeability for fluorescein (332 Da) in infected cell monolayers was more pronounced in the co-culture compared to the monoculture condition (Figure 5A). Furthermore, we found a significant increase in permeability to FITC-dextran (4 kDa) in C. concisus-infected cell monolayers under co-culture conditions, but not in the monoculture condition (Figure 5B).
The differential pattern of the increase in permeability to macromolecules of different size indicates that the cellular structures could mediate these effects. Under monoculture conditions, C. concisus-infected cell monolayers were not permeable to FITC-dextran (4 kDa), but permeable to fluorescein (332 Da). This functional permeability pattern points to the delocalization of occludin in bTJs and/or apoptosis induction. By contrast, the increase in epithelial permeability to both fluorescein and FITC-dextran in the co-culture infection points to a loss of tricellulin in the tTJs of polarized epithelial cell monolayers (Figure 4). However, the possibility of increased apoptotic ratio should also be taken into account.

2.5. Epithelial Apoptosis in HT-29/B6-GR/MR Cells after Campylobacter concisus Infection

We determined the extent of epithelial apoptosis in the epithelial monolayers 48 h after C. concisus infection. Hence, we quantified apoptosis induction with fluorescence microscopy by counting apoptotic cells in the assay of terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) in both monoculture and co-culture conditions. Higher numbers of apoptotic cells were detected in the infected cell monolayers in both monoculture and co-culture conditions by microscopic assessment in low-power fields (Figure 6A).
The percentage of apoptotic cells was higher in C. concisus-infected cell monolayers in both monoculture and co-culture conditions when compared to controls (Figure 6B). Interestingly, there was no significant change in the apoptosis rate of the cell monolayers infected with C. concisus in the co-culture condition compared to the monoculture condition (Figure 6B). Apoptosis induction in the epithelium by the bacteria per se appears to be a major contributor to the barrier defect, along with the immune cell response.

2.6. Inflammatory Response by M1-Macrophage-Like THP-1 Cells after Campylobacter concisus Infection in the Co-Culture Setting

To ascertain the effect of immune cells in the colonic epithelial barrier dysfunction caused by C. concisus, we measured the release of cytokines from the stimulated THP-1 cells 48 h after C. concisus infection in co-culture condition. In the culture media of the basal side of the infected cell monolayers, we assessed the release of different cytokines by Cytometric Bead Array (CBA). We found a marked increase in the release of the pro-inflammatory cytokines TNF-α, IL-1β, and IL-6, after infection, when compared to controls (Figure 7). There was also an anti-inflammatory response elicited by increased levels of IL-10 released from the THP-1 cells after C. concisus infection (Figure 7). Furthermore, the levels of the pro-inflammatory cytokines IFN-γ, IL-13, IL-2, and IL-17A remained unaltered (Figure S1).

2.7. Cell Viability in Colonic Epithelial Cell Monolayers after C. concisus Infection

We evaluated the viability of polarized epithelial cell monolayers 48 h after C. concisus infection. The infected HT-29/B6-GR/MR cell monolayers along with controls were subjected to the cell viability CCK-8 assay (Cell Counting Kit-8). The overall host cell viability of the C. concisus-infected cell monolayers in monoculture and co-culture conditions was not significantly different compared to controls (Figure 8).
In addition to the cell monolayers, we also determined the cell proliferation rate of HT-29/B6-GR/MR cells and M1-macrophage-like THP-1 cells at 48 h p.i. with different MOI of C. concisus ranging from 25 to 1000. We identified that the cell proliferation rate of both HT-29/B6-GR/MR and M1-macrophage-like THP-1 cells was decreased only with an MOI of 500 and 1000 compared to controls (Supplementary Figure S2A,B). We found no significant changes in the cell proliferation rate of HT-29/B6-GR/MR cells in MOI ranging from 25 to 400 (Figure S2A). Interestingly, we also found that there was a significant increase in the cell proliferation rate of the THP-1 cells after C. concisus infection at an MOI of 25, 50, 100, and 200 (Figure S2B), indicating a proliferation stimulus on the immune cells by C. concisus.

3. Discussion

As our first main result, we showed that Campylobacter concisus (strain AAuH 37 UC oral, an oral isolate from an UC patient in Denmark [40]) induces intestinal epithelial barrier dysfunction with a significant decrease in TER of the infected HT-29/B6-GR/MR cell monolayers at 24 h and 48 h following infection. The colonic epithelial barrier dysfunction induced by the C. concisus isolate confirmed its enteric pathogenic potential in the context of intestinal inflammation and diarrhea as described in other studies [34,41]. We performed the present study on barrier function only with a single strain, as our previous study revealed that several strains of C. concisus from healthy and diseased patients did not differ in their ability to lower intestinal epithelial barrier function regardless of the isolation sites [29].
It has also been reported that there was a significant increase in pro-inflammatory cytokines TNF-α, IL-1β, IL-12, and the chemokine IL-8 released by macrophages following C. concisus infection [28,41]. To date, the relative contribution and the inherent mechanisms between the subepithelial immune activation and intestinal epithelial barrier dysfunction induced by C. concisus have not been explored in detail. To investigate this causal link, we used an in vitro co-culture model of colonic epithelial cell monolayers with M1-macrophages, as described previously [27,36].

3.1. Immune-Mediated Aggravation of Campylobacter concisus-Induced Colonic Epithelial Barrier Dysfunction

Using this model, we observed a marked decrease in TER of C. concisus-infected cell monolayers, which was more pronounced in co-culture compared to monoculture condition at 48 h post infection. This again highlights the important role of macrophage activation and release of pro-inflammatory cytokines, TNF-α, IL-1β, and IL-6 in modulating C. concisus-induced colonic epithelial barrier dysfunction. This macrophage activation in our model represents a non-specific immune response. Such activation of macrophages with migration into the rectal epithelium was also observed in vivo in an ultrastructural analysis on Campylobacter spp.-infected patients with acute colitis [42]. Furthermore, in secondary abiotic IL-10-/- mice challenged with human fecal microbiota, peroral C. coli infection promoted activation of macrophages in conjunction with the increased TNF-α secretion in colonic tissues 21 days post infection [43], a finding which supports the view of the immune activation being a central mechanism of the functional defects following the infection. In our present in vitro study, we also observed an activation of M1-macrophage-like THP-1 cells 48 h following C. concisus infection depicted through an increase in cell proliferation rate at different ranges of MOI from 25 up to 200 (Figure S2B). Interestingly, this is not unique to the infection with C. concisus, since in the human immunodeficiency virus (HIV) infection with compromised intestinal epithelial barrier function and leak-flux diarrhea, macrophages were also activated and elicited an inflammatory response through an increased release of the pro-inflammatory cytokines TNF-α, IL-1β, IFN-α, and IFN-γ [44]. Also, in bacterial infections of enteropathogenic E. coli (EPEC) [45] and C. jejuni [39], intestinal epithelial barrier dysfunction was accompanied by inflammatory responses.
TNF-α is a major pro-inflammatory cytokine released from the intestinal epithelium in response to infections with diarrheal pathogens like Vibrio cholerae, Salmonella enterica, and C. jejuni [39,46,47]. TNF-α, either alone or in combination with IFN-γ and IL-13, can regulate colonic epithelial barrier function in vitro and this mimics the differential regulation of TJ proteins observed in active IBD patients [48,49,50]. IL-1β plays a dual role as it helps to control the infection by the host-inflammatory response, and at the same time permits the entry of luminal content into the serosal compartment of the epithelium to favor inflammation [51]. Interestingly, C. jejuni elicited inflammatory responses through IL-1β release from THP-1 monocytes, macrophages, and inflamed colon mucosae of IL-10-/- mice [36,52,53]. The inflammatory response of macrophages from C. jejuni infection involved TNF-α and IL-6 in addition to IL-1β to induce epithelial apoptosis affecting only claudin-1 expression in TJ of colonic epithelial cells [36]. In this study, we observed a similar inflammatory response by macrophages after C. concisus infection through the release of TNF-α, IL-6, and IL-1β, although in higher concentrations compared to C. jejuni [36,37].
In addition to the pro-inflammatory cytokines released by macrophages after C. concisus infection in the co-culture setting, we also detected an increase in the anti-inflammatory cytokine IL-10. This increase in IL-10 release after C. concisus infection could be induced by cAMP responsive element binding protein 1 (CREB1), as CREB1 upregulated mRNA expression of IL-10 after C. concisus infection in differentiated THP-1 macrophages, which was discerned by RNA-Seq data [54]. Furthermore, IL-10 constitutively produced by intestinal macrophages play a pivotal role in controlling excessive innate immune activation and preventing tissue damage after acute bacterial infection [55]. Beyond the immune activation promoted by C. concisus, the local inflammatory responses in small and large intestinal segments of IBD patients might enhance the colonization of C. concisus in the intestine [34,35], leading to a vicious circle of inflammation and epithelial TJ disruption. Taken together, the epithelial TJ alterations and/or apoptosis represent the molecular correlates of the pronounced intestinal epithelial barrier dysfunction resulting from macrophage activation by C. concisus.

3.2. Tight Junction Modifications in Colonic Epithelial Barrier Dysfunction Induced by Campylobacter concisus

Although C. concisus was described as an emerging enteropathogen [28,29,56], sparse scientific information is available so far on TJ changes leading to intestinal epithelial barrier dysfunction. Previous studies identified that C. concisus induces epithelial barrier dysfunction via moderate TJ changes and epithelial apoptosis induction [28,29]. In the present study, we investigated the TJ changes in colonic epithelial cells (HT-29/B6-GR/MR) in more detail, especially under the influence of the immune activation.

3.2.1. Functional Loss of Occludin in Colonic Epithelial Cells in Co-culture with Macrophages after Campylobacter concisus Infection

The expression of occludin was downregulated in membrane fractions of Caco-2 cells 48 h after C. concisus infection, whereas the expression of total occludin remained unaltered in a previous study [28]. In the present study, we also found that the expression of total occludin remained unaltered in HT-29/B6-GR/MR cell monolayers at 48 h after C. concisus infection in monoculture condition. However, under the co-culture condition with immune activation, we also ascertained a downregulation in the protein expression of total occludin after C. concisus infection. In addition, we detected a subcellular redistribution of occludin from the bTJ in HT-29/B6-GR/MR cell monolayers after C. concisus infection in co-culture condition. The reduction in occludin expression and its redistribution from bTJ following C. concisus infection might also result from an increased release of IL-6 and IL-1β by M1-macrophages, as reported elsewhere [57,58,59]. In addition to IL-6 and IL-1β, earlier studies reported TNF-α-induced epithelial barrier dysfunction in rat colon via downregulation of occludin and E-cadherin [60] and in DSS-induced colitis in mice [61]. To summarize, it is reasonable to conclude that the inflammatory response of M1-macrophage-like THP-1 cells facilitates downregulation of occludin expression and its dissociation from bTJ in colonic epithelial cells after C. concisus infection. Furthermore, occludin knock-down in MDCK cell monolayers revealed that occludin regulates paracellular permeability, with an increase in the paracellular flux of macromolecules up to 40 kDa in the absence of occludin [15]. Hence, the downregulation of occludin and its dissociation from the bTJ in HT-29/B6-GR/MR cell monolayers could be one contributing factor for an increase in epithelial permeability to small (fluorescein, 332 Da) and large (FITC-dextran, 4 kDa) macromolecules in infected cell monolayers in co-culture condition compared to monoculture condition.

3.2.2. Campylobacter concisus Induces Tricellulin Downregulation and Redistribution from Tricellular Tight Junctions of Colonic Epithelial Cells in Co-culture with Macrophages

Tricellulin is an integral component of the intestinal epithelium regulating barrier function for macromolecules [19,20]. In co-culture condition, we observed a reduction in the protein expression of tricellulin and a profound subcellular redistribution 48 h after C. concisus infection. The tTJ was first identified as a bacterial target in MDCK cell monolayers, where tricellulin knock-down prevented the intercellular movement and shedding of Shigella, which revealed tricellulin as a receptor for bacterial invasion [62]. Direct interaction of C. concisus with the tTJ, as shown for Shigella, is possible and should be addressed in future investigations. Furthermore, in intestinal epithelial barrier damage by EPEC, tricellulin expression was reduced and a random re-distribution of tricellulin off tTJ was observed in Caco-2 cell monolayers at 4 h p.i. [63]. We identified for the first time that Campylobacter spp. infection downregulates tricellulin expression under the influence of immune activation to promote barrier dysfunction in the intestinal epithelial cells. Even in the absence of an inflammatory response, as present in monoculture cell monolayers, we observed a redistribution of tricellulin from the tTJ in a subpopulation of cells after C. concisus infection. However, a profound general redistribution of tricellulin from the tTJ was observed 48 h after C. concisus infection only under the influence of the inflammatory response from macrophages. In contrast to C. concisus, tricellulin expression remained unaltered in C. jejuni infections in our co-culture model of colonic epithelial cells with M1 macrophages, where inhibition of epithelial apoptosis induction attenuated the barrier dysfunction induced by C. jejuni [37]. However, tricellulin expression in the co-culture model was investigated only at an early time point of 22 h after C. jejuni infection. Investigations of tricellulin expression 48 h after C. jejuni infection in co-culture condition might have a different outcome. EpSG1, an effector molecule of type-III secretion system (T3SS) in EPEC, was found to exhibit a selective role in the downregulation of tricellulin via disruption of microtubular filaments [63]. Since a number of clinical isolates of C. concisus possess type-VI secretion system (T6SS) genes, which code for a complete T6SS apparatus [64,65], future studies should investigate the role of T6SS-dependent effector molecules in intestinal epithelial barrier dysfunction induced by C. concisus with respect to tricellulin downregulation.
In our present study, the loss of tricellulin from the tTJ was accompanied by a nine-fold increase in the permeability to FITC-dextran (4 kDa) in C. concisus-infected colonic epithelial cells in co-culture compared to monoculture condition. This correlates with the disease pathomechanisms in the colon mucosae of UC patients [66] and ileal biopsies of monozygotic twins with CD [67], where tricellulin downregulation was accompanied by an increase in paracellular permeability to 4 kDa FITC-dextran. This allows an influx of larger macromolecules and bacterial antigens into the intestinal mucosa to promote inflammatory processes. In UC patients, IL-13 was determined as the pro-inflammatory cytokine which induced tricellulin downregulation and its redistribution from the tTJ [66]. However, in C. concisus infection in our co-culture setting, IL-13 release from M1 macrophages was not significantly altered compared to controls (Figure S1). Hence, C. concisus has to be assumed to promote an IL-13-independent tricellulin downregulation and redistribution from the tTJ in colonic epithelium, e.g., through macrophage-derived TNF-α, a mechanism similar to the compromised epithelial barrier function in the duodenal mucosa of Whipple’s Disease patients [68]. Thus, with immune cells that were capable of secreting IL-13 in other macrophage models like PBMCs or in the in vivo experiments, the barrier dysfunction via tricellulin dysregulation could be even more pronounced. Tricellulin loss in the tTJ after C. concisus infection in our co-culture experiments could be attributed to the downregulation and redistribution of occludin from the bTJ, as loss of occludin notably affects the localization of tricellulin in the tTJ [15,20]. However, the molecular mechanism that governs the tricellulin downregulation or its redistribution resulting from C. concisus infection in co-culture condition has to be elucidated in future studies.

3.2.3. The Role of Claudins in Campylobacter concisus-Induced Colonic Epithelial Barrier Dysfunction

As a structural correlate of the epithelial barrier dysfunction induced by C. concisus in HT-29/B6 cells, a previous study identified a reduction in protein and mRNA expression of claudin-5 [29]. It was accompanied by an increase in epithelial permeability to 332 Da fluorescein, but not to 4 kDa FITC-dextran [29]. In our present investigation, we observed a decrease in claudin-5 protein expression 48 h after C. concisus infection in both monoculture and co-culture conditions. It points to a mechanism of TNF-α-dependent decrease in claudin-5 in the colon mucosae of lymphocytic colitis patients [9], as TNF-α release was evident in colonic epithelial HT-29 cells following C. concisus infection [28]. However, in monoculture condition, C. concisus-infected cell monolayers showed a 6.6-fold increase in epithelial permeability to fluorescein (332 Da). Hence, the reduction in claudin-5 expression by C. concisus could also influence the increase in epithelial permeability to the small macromolecule (fluorescein, 332 Da).
We detected a decrease in the protein and mRNA expression of claudin-8 in HT-29/B6-GR/MR cell monolayers 48 h after C. concisus infection in our previous study, in which regulation of claudin-8 expression was influenced by stimulation with dexamethasone (D), butyrate-sodium salt (B), and aldosterone (A) [5]. In our present study, claudin-8 protein expression remained unaltered 48 h after C. concisus infection in HT-29/B6-GR/MR cell monolayers, presumably because they were not pre-stimulated with DBA. Claudin-1 expression was increased 48 h after C. concisus infection in HT-29/B6-GR/MR cell monolayers in both monoculture and co-culture conditions and this resembled the increase in claudin-1 expression 48 h following C. fetus and C. coli infections [69]. This phenomenon of an increase in claudin-1 in spite of a decrease in TER is called claudin-1 paradox. It is due to a redistribution of the (increased) claudin-1 off the tight junction domain of the epithelial cells as shown in the C. jejuni infection in the colonic epithelium [37,38]. The reduction in TER and upregulation of claudin-1 expression results from the pro-inflammatory cytokine TNF-α via NF-κB [70]. In contrast to the increase in claudin-1 expression after C. concisus infection in colonic epithelium, a recent study reported claudin-1 downregulation with a concomitant barrier dysfunction and an increase in inflammation in atopic dermatitis [71]. However, in the context of intestinal inflammation, claudin-1 expression was increased in the colon mucosae of IBD patients [72]. Furthermore, an increase in claudin-1 expression in the intestinal epithelium was also implicated in inflammation-induced colon tumorigenesis [73]. However, it is unknown whether claudin-1 has self-regulatory properties in this respect or has been subject to expression regulation regardless of the inflammation. At this point, we can speculate that the claudin-1 redistribution off the tight junction domain into intracellular compartments in the colon induces a counter-regulation with claudin-1 transcription upregulation to maintain barrier function.

3.3. Epithelial Apoptosis in the Regulation of Intestinal Epithelial Barrier Dysfunction Induced by Campylobacter concisus

Epithelial apoptosis was identified as a major determinant in the colonic epithelial barrier dysfunction induced by C. concisus in HT-29 cells at 48 h p.i., which supports the concept of dysfunction through an increase in the paracellular flux of ions and small macromolecule (fluorescein, 332 Da), but not large macromolecule (FITC-dextran, 4 kDa) [29]. This leak-flux pathomechanism in intestinal epithelium with up-regulated epithelial apoptoses was also detected in duodenal biopsies of patients with Giardia lamblia infection [74]. Furthermore, this was evident in C. fetus infection in HT-29 cells. However, C. fetus promoted an increase in permeability to 10 kDa FITC-dextran through focal leaks in cell monolayers [68]. In a recent study with a co-culture model of colonic epithelial cells with macrophages, C. jejuni was also found to induce epithelial apoptosis in the colonic epithelium with an increase in paracellular flux for 4 kDa FITC-dextran at 22 h p.i. [37]. Therefore, we aimed to determine if epithelial apoptosis contributes to the pronounced colonic epithelial barrier dysfunction after C. concisus infection in our co-culture model.
There were no significant changes in the number of apoptotic cells between the cell monolayers infected in monoculture and co-culture conditions, although we found a significant increase in the paracellular flux of 4 kDa FITC-dextran in co-culture infected cell monolayers compared to monoculture infected monolayers. This suggests that an increase in large macromolecule (FITC-dextran, 4 kDa) flux was caused by the disruption of tricellulin in the tTJ, but not by epithelial apoptosis. However, the epithelial apoptotic rate was significantly higher in infected cell monolayers compared to controls in both monoculture and co-culture conditions. Hence, this could be one contributing factor along with dysfunctional occludin and claudin-5 to influence the small macromolecule (fluorescein, 332 Da) flux across the colonic epithelium. Most importantly, the necrotic cell death mechanisms did not influence the barrier dysfunction of C. concisus-infected HT-29/B6-GR/MR cell monolayers, as no significant differences were observed in cell cytotoxicity between the controls and infected monolayers (Figure 8). As a piece of supportive evidence, we also show that the proliferation of HT-29/B6-GR/MR cells in 96-well plates was not significantly altered at 48 h p.i. with respect to controls up to an MOI of 400 (Figure S2A).
Taken together, C. concisus induce epithelial barrier dysfunction in colonic epithelium through epithelial apoptosis and claudin-5 downregulation in the absence of inflammatory response from macrophages. C. concisus infection also facilitates flux of ions and smaller macromolecules like fluorescein. It confirms the concept of a leak-flux pathomechanism exhibited by C. concisus, as proposed in previous studies. Most importantly, with the macrophage activation and cytokine induction, which resembles the in vivo situation of the inflamed colon in campylobacteriosis, the colonic epithelial barrier damage promoted by C. concisus exacerbates via occludin and tricellulin disruption. Tricellulin loss in the tTJ could create a large channel in the central tube of the epithelial tricellular contacts in the intestine. It allows the influx of large macromolecules, for instance—bacterial toxins through the tTJ, and thereby exacerbates intestinal inflammation in campylobacteriosis or IBD patients leading to watery or bloody diarrhea.

4. Materials and Methods

4.1. Cell Culture

We used HT-29/B6-GR/MR cell line (classical HT-29/B6 cell line, stably transfected with glucocorticoid and mineralocorticoid-receptors) to analyze the epithelial barrier function following C. concisus infection. HT-29/B6-GR/MR cells were cultured in Roswell Park Memorial Institute (RPMI) media (Sigma Aldrich, St. Louis, MO, USA) for one week at 37 °C in a humidified atmosphere (95% air/5% CO2). RPMI media were supplemented with 10% fetal calf serum (FCS; Gibco, Carlsbad, CA, USA), 1% penicillin/streptomycin (Gibco, Carlsbad, CA, USA), 500 IU/mL G418 (Merck Millipore, Billerica, MA, USA), and 200 μg/mL hygromycin B (Biochrom GmbH, Berlin, Germany). The human monocyte leukemia cell line THP-1 (ATCC TIB-202) was provided by Dr. Verena Moos, Medical Department, Division of Gastroenterology, Infectiology, and Rheumatology, Charité–Universitätsmedizin Berlin, Berlin, Germany. The THP-1 monocytes were grown as suspended cells for seven days in RPMI media supplemented with heat-inactivated 10% FCS and 1% penicillin/streptomycin. The cells were then subjected to centrifugation at 130 g for 10 min at 22 °C. The cell pellets were re-suspended in RPMI media supplemented with heat-inactivated 10% FCS without any antibiotic supplements. The cell count was determined and 1.8 × 105 cells were seeded into individual compartments of 12-well plates (Falcon Polystyrene Microplates, Thermo Scientific, Waltham, MA, USA) and treated with 100 nM phorbol 12-myristate 13-acetate (PMA; Sigma Aldrich St. Louis, MO, USA) for 24 h to differentiate them into M1 type macrophages.
The confluent HT-29/B6-GR/MR cells in a culture flask were trypsinized and seeded on Millicell PCF filters of 3 μm pore size (Merck Millipore, Billerica, MA, USA). Seven to nine days old HT-29/B6-GR/MR cell monolayers were washed and incubated overnight in RPMI media supplemented with heat-inactivated 10% FCS without any antibiotic supplements. In parallel, M1-macrophages differentiated from THP-1 monocytes were washed and incubated in RPMI media with heat-inactivated 10% FCS without any antibiotic supplements. After overnight incubation with antibiotic-free medium, the HT-29/B6-GR/MR cell monolayers on 3 µm PCF filters were placed over the single wells of 12-well plates containing M1-macrophage-like THP-1 cells (Figure 9). This depicts the leaky gut in vitro co-culture model of colonic epithelial cells with M1-macrophages on the basal side as described in a previous study [36]. In parallel, we also used monoculture HT-29/B6-GR/MR cell monolayers in 12-well plates without THP-1 cells.

4.2. Transepithelial Electrical Resistance Measurements and Campylobacter concisus Infection

The transepithelial electrical resistance (TER) was recorded with chop-stick electrodes (STX2, World Precision Instruments, Sarasota, FL, USA) and a volt-ohm meter (Institute of Clinical Physiology, Charité–Universitätsmedizin, Berlin, Germany) in both monoculture and co-culture (with M1-macrophage-like THP-1 cells on the basal side) cell monolayers under sterile conditions. The cell monolayers were infected with C. concisus (C. concisus AAuH 37 UC oral [40]) on the apical and basal sides of the cell monolayers at a multiplicity of infection (MOI) of 200 (Figure 9). After infection, the cell monolayers were incubated in a microaerophilic and H2-containing atmospheric condition at 37 °C, as described in our previous study [5]. TER was recorded at 24 h and 48 h p.i.. The oral isolate of C. concisus from a UC patient (C. concisus AAuH 37 UC oral) was provided by Dr. Hans Nielsen, Department of Clinical Microbiology/Clinical Medicine, Aalborg University Hospital/Aalborg University, Aalborg, Denmark.

4.3. Western Blotting to Determine the Expression of Different Tight Junction Proteins

The controls and infected cell monolayers in both monoculture and co-culture conditions were washed twice with PBS. The cells were then scraped out carefully from the cell monolayers and subjected to lysis for one hour in ice-cold condition with whole-cell lysis buffer (150 mM NaCl, 10 mM Tris buffer pH of 7.5, 0.5% Triton X-100, and 0.1% SDS). After lysis, the cells were centrifuged at 13,000 rpm for 30 min at 4 °C and the supernatants were collected in separate tubes. The supernatants were then subjected to sonication with 15 pulses at brief intervals of 3 s for every 5 pulses. After sonication, the cells were again centrifuged for 13,000 rpm for 30 min at 4 °C. The supernatants were then transferred to fresh vials and maintained in ice-cold conditions. The concentration of the proteins was estimated by Pierce bicinchoninic acid (BCA) assay (Thermo Scientific, Waltham, MA, USA) according to the manufacturer’s instruction. Protein samples were resolved using 10% SDS-PAGE gels and 12.5% SDS-PAGE gels, and 15 µg of proteins were used from each sample. The resolved proteins were electro-transferred to PVDF nitrocellulose membranes (Thermo Scientific, Waltham, MA, USA) with Wet/Tank Blotting system (Bio-Rad Laboratories, Inc., Hercules, CA, USA) according to the manufacturer’s instruction.
PVDF nitrocellulose membranes were subjected to incubation shaking with a blocking solution containing 1% polyvinylpyrrolidone (PVP-40; Sigma Aldrich, St. Louis, MO, USA) in tris-buffered saline (TBS) supplemented with 0.05% Tween-20 at room temperature (RT) for 1 h to avoid unspecific protein signals. Following this, we incubated the membranes with primary antibodies rabbit (Rb) anti-claudin-1, -2, -5, -7, and -8 (Invitrogen, Carlsbad, CA, USA), mouse (M)-anti-claudin-4 (Invitrogen, Carlsbad, CA, USA), Rb-anti-occludin (Sigma Aldrich, St. Louis, MO, USA), Rb anti-tricellulin (Invitrogen, Carlsbad, CA, USA), M-anti-β-actin (Sigma Aldrich, St. Louis, MO, USA), and M-anti-GAPDH (Merck KGaA, Darmstadt, Germany) overnight at 4 °C. Membranes were then incubated with appropriate secondary antibodies (peroxidase-conjugated goat anti-Rb and goat-anti-M, Jackson ImmunoResearch, Ely, UK) at RT for 2 h. The membranes were then detected for the bands of specific proteins with a chemiluminescence solution (Thermo Scientific, Waltham, MA, USA) using the FUSION FX7 system (Vilber Lourmat Deutschland GmbH, Eberhardzell, Germany). Protein bands were quantified by ImageJ software (Rasband, W. S., ImageJ, National Institute of Health (NIH), Bethesda, MD, USA). Densitometric analysis of the Western blots was performed by normalizing the band intensity of TJ proteins to their respective β-actin or GAPDH band intensities.

4.4. Immunofluorescence Staining and Confocal Laser-Scanning Microscopy to Determine Tight Junction Protein Localization

Forty-eight hours after C. concisus infection, HT-29/B6-GR/MR cell monolayers under monoculture and co-culture conditions were analyzed for TJ localization along with the controls. The cell monolayers in 3 μm PCF filters were fixed using 2% paraformaldehyde (PFA; Electron Microscopy Sciences, Hatfield, PA, USA) at RT for 20 min. After fixation, the cells were quenched with 25 mM Glycine (Biomol GmBH, Hamburg, Germany). The fixed cells were washed twice with PBS (with Ca2+/Mg2+) and permeabilized with 0.5% Triton X-100 (Sigma Aldrich, St. Louis Missouri, MO, USA) for 7 min at RT. The permeabilized cells were washed and incubated with blocking solution (1% (v/v) goat serum (Gibco, Carlsbad, CA, USA)) diluted in PBS (with Ca2+/Mg2+) at RT for 30 min. The primary antibodies, Rb-anti-occludin (Thermo Scientific, Waltham, MA, USA), Rb-anti-Tricellulin (Invitrogen, Carlsbad, CA, USA), and M-anti-human ZO-1 (BD Biosciences, Franklin Lakes, New Jersey, USA) were diluted with the blocking solution to optimal concentrations. After blocking, the cells were incubated with primary antibodies at 37 °C for 45 min. Then, the cell monolayers were incubated with secondary antibodies, goat anti-Rb green, Alexa Fluor Plus 488 nm and goat-anti-M red, Alexa Fluor 594 nm (Invitrogen Carlsbad, CA, USA) for 45 min at 37 °C. Following this, the cell monolayers’ nuclei were stained with 4′-6-diamidino-2-phenylindole dihydrochloride (DAPI; Roche AG, Basel, Switzerland) at a dilution of 1:1000 in blocking solution. Later, the cell monolayers were dried and mounted on a glass slide using the mounting solution ProTaq Mount Fluor (Biocyc, Luckenwalde, Germany) and fixed with coverslips. The control and the infected cell monolayers were assessed for localization and redistribution of TJ proteins occludin and tricellulin, co-stained with zonula occludens protein-1 (ZO-1) using confocal laser-scanning microscopy (CLSM, Zeiss LSM 780, Jena, Germany). The individual Z-stacks of the cell monolayers were recorded using the laser scan function.

4.5. Epithelial Permeability Assay

Forty-eight hours after C. concisus infection, the HT-29/B6-GR/MR cell monolayers along with controls were incubated with RPMI medium supplemented with 50 µg/mL gentamycin for 2 h at 37 °C. After incubation with gentamycin-supplemented RPMI media, the fluorescent markers of different molecular size, fluorescein (332 Da; 100 µM) and FITC-dextran (4 kDa; 200 µM) were used to measure the paracellular permeability of the epithelial cell monolayers. The permeability of the cell monolayers was measured through unidirectional flux for fluorescein and FITC-dextran in 12-well plates at 37 °C over 1–2 h. In fluorescein flux measurements, the samples from the basolateral side were obtained every 15 min up to 1 h. For FITC-dextran, the samples were obtained every 30 min up to 2 h. The fluorescence signals were measured in a spectrophotometer (Tecan GmbH, Maennedorf, Switzerland) at optimal wavelengths to calculate the flux of fluorescein and FITC-dextran. The permeability was then calculated from flux over concentration difference.

4.6. Quantification of Epithelial Apoptosis

To detect epithelial apoptosis in C. concisus-infected colonic epithelial cell monolayers, we used TUNEL assay kit (In situ Cell Death Detection Kit, Roche AG, Mannheim, Germany). We stained the cell monolayers with the enzyme solution, terminal deoxynucleotidyl transferase (TdT) according to the manufacturer’s instructions. Nuclei were then stained by DAPI (Roche AG, Basel, Switzerland) at the optimum concentration. The cellular apoptosis was visualized using confocal laser-scanning microscopy (CLSM; Zeiss LSM 780, Jena, Germany). The apoptotic cells were then counted in randomly picked regions, with 5 to 8 regions in controls and infected cell monolayers at a low power field of approximately 150 to 200 cells. Then, the percentage of TUNEL-positive cells was estimated from the ratio of apoptotic or TUNEL-positive cells to the total number of cells in the regions picked for quantification.

4.7. Cytometric Bead Array to Measure the Cytokine Release

We measured the pro-inflammatory cytokines released from M1-macrophage-like THP-1 cells 48 h after C. concisus infection in co-culture cell monolayers. The media from the basal compartment of the C. concisus-infected and control cell monolayers were collected from 12 well plates. This was subjected to analysis of multiple cytokines (IL-1β, IL-13, IL-4, IL-6, IL-10, IL-17A, IFN-γ, and TNF-α) with capture bead technology of human cytometric bead array kit (CBA; BD Biosciences Human Th1/ Th2/Th17 Kit and Flex Set IL-13 and IL-1β, Franklin Lakes, NJ, USA) according to the manufacturer’s protocol. The cytokines were then measured with flow cytometry by Fluorescence-activated Cell Sorting (FACS) Canto II (BD Biosciences; Franklin Lakes, NJ, USA). The data from the cytokine measurements were analyzed with FACP Array software v3.0 (BD Biosciences, Franklin Lakes, NJ, USA).

4.8. Cell Viability Assay

The cell proliferation rate of HT-29/B6-GR/MR cell monolayers 48 h after C. concisus infection (MOI = 200, on apical and the basal side) were evaluated by CCK-8 assay (Cell Counting Kit-8, Thermo Scientific, Waltham, MA, USA). In addition to this, we also determined the individual cell proliferation rate of HT-29/B6-GR/MR cells and M1-macrophage-like THP-1 cells in 96-well plates. HT-29/B6-GR/MR cells were seeded into 96-well plates and incubated overnight at 37 °C in a humidified atmosphere (95% air/5% CO2). The cells were washed and incubated overnight with antibiotic-free RMPI medium. In parallel, THP-1 monocytes were differentiated into M1 macrophages with PMA for 24 h. Following this, M1 macrophages were also washed and incubated overnight with antibiotic-free RPMI medium. Then, both HT-29/B6-GR/MR and THP-1 cells were infected with C. concisus at MOI of 25, 50, 100, 200, 400, 500, and 1000. Forty-eight hours post-infection, the cell viability was determined by CCK-8 assay according to the manufacturer’s instructions. We added 10 µL of CCK-8 solution (WST-8 [2-(2-methoxy-4-nitrophenyl)-5-(2, 4-disulfophenyl)-2H-tetrazolium, monosodium salt]) to 100 µL of cell suspensions in 96-well plates. Absorbance values were recorded using a spectrophotometer (Tecan GmbH, Maennedorf, Switzerland) at 450 nm, with a reference wavelength of 600 nm 1–2 h after addition of the CCK-8 solution. The absorbance values of infected cells were normalized with controls and the percentage of cell proliferation at different MOI of C. concisus was calculated.

4.9. Statistical Analysis

All data are expressed as the mean value ± standard error of the mean (SEM). Statistical analyses were performed with GraphPad Prism (GraphPad Software version 5.0, Inc., San Diego, CA, USA). Unpaired t-test was used to compare the mean values of the independent groups. To compare the data sets with unequal variances, we used unpaired t-test with Welch’s correction. p < 0.05 was considered statistically significant.

Supplementary Materials

The following are available online at https://www.mdpi.com/1422-0067/22/4/2043/s1, Figure S1. Cytokines released from THP-1 cells in co-culture with cell monolayers. Figure S2. Cell viability in Campylobacter concisus-infected HT-29/B6-GR/MR cells and macrophage-like THP-1 cells 48 h post infection.

Author Contributions

Conceptualization, P.K.N., R.B., and J.-D.S.; Data curation, P.K.N., F.D.L.d.S., and R.B.; Formal analysis, P.K.N., F.D.L.d.S., and R.B.; Funding acquisition, J.-D.S., and R.B.; Investigation, P.K.N., R.B., and J.-D.S.; Methodology, P.K.N., F.D.L.d.S., R.B., and J.-D.S.; Project administration, J.-D.S., and R.B.; Resources, R.B., and J.-D.S.; Supervision, R.B., and J.-D.S.; Validation, R.B., and J.-D.S.; Visualization, P.K.N., and R.B.; Writing-original draft, P.K.N.; Writing-review and editing, R.B., and J.-D.S. All authors have read and approved the final manuscript.

Funding

This research was funded by the German Research Foundation (DFG, GRK 2318) to J.-D.S. This work was supported by the German Federal Ministry of Education and Research (BMBF) in the frame of the zoonoses research consortium PAC-Campylobacter to R.B. and J.-D.S. (project IP8/01KI2007D). We acknowledge support from the German Research Foundation and the Open Access Publication Fund of the Charité–Universitätsmedizin Berlin.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

No big data repositories needed. The raw data supporting the findings of this manuscript will be made available by the corresponding author, R.B., or the first author, P.K.N., to any qualified researcher upon reasonable request.

Acknowledgments

We acknowledge Anja Fromm and In-Fah Maria Lee for their excellent technical support in the experiments involved in this study. We would also like to extend our gratitude to Jörg Piontek, Institute of Clinical Physiology, Charité—Universitätsmedizin Berlin for his useful inputs in the technical aspect of image analysis in confocal laser-scanning microscopy.

Conflicts of Interest

The authors declare no conflict of interest.

Abbreviations

ATCCAmerican Type Culture Collection
BCABicinchoninic acid
cAMPAdenosine 3′,5′ -cyclic monophosphate
CBAC Cytometric Bead Array
CCK-8Cell counting kit-8
CDCrohn’s Disease
CFUColony forming units
CLSMConfocal laser scanning microscopy
CREB1cAMP responsive element binding protein 1
CRPC-Reactive Protein
CWDClassical Whipple’s Disease
DAPI4′-6-diamidino-2-phenylindole dihydrochloride
DBADexaDexamethasone, butyrate-sodium salt, and aldosterone
DSS Dextran sodium sulfate
EDTAEthylenediaminetetraacetic acid
EGTAEthylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid
ENaCEpithelial sodium channel
EPECEnteropathogenic Escherichia coli
ERKExtracellular signal-regulated kinases
FACSFluorescence-Activated Cell Sorting
FCSFetal calf serum
FITCFluorescein isothiocyanate
GAPDHGlyceraldehyde 3-phosphate dehydrogenase
GRGlucocorticoid receptor
HIVHuman immunodeficiency virus
IBDInflammatory bowel disease
IFNInterferon
ILInterleukin
LPSLipopolysaccharide
MOIMultiplicity of infection
MRMineralocorticoid receptor
mRNAmessengerRNA
NF-κBNuclear factor kappa light chain enhancer of activated B cells
p.i.Post infection
PBMCPeripheral blood mononuclear cells
PBSPhosphate buffered saline
PCFPolycarbonate membrane filters
PFAParaformaldehyde
PMAphorbol 12-myristate 13-acetate
PVDFPolyvinylidene fluoride
PVPPolyvinylpyrrolidone
RNA-SeqRNA sequencing
RPMIRosewell Park Memorial Institute
RTRoom temperature
SDSSodium dodecyl sulfate
T3SSType-III secretion system
T6SSType-VI secretion system
TBSTris-buffered saline
TERTransepithelial electrical resistance
TJTight junction
tTJTricellular TJ
TNFTumor necrosis factor
TUNELTerminal deoxynucleotidyl transferase dUTP nick end labeling
UCUlcerative colitis
ZO-1Zonula occludens protein-1

References

  1. Tanner, A.C.; Badger, S.; Lai, C.-H.; Listgarten, M.A.; Visconti, R.A.; Socransky, S.S. Wo Zinella gen. nov., WoZinella succinogenes (Vibrio succinogenes Wolin et al.) comb. nov., and Description of Bacteroides gracilis sp. nov., Wolinella recta sp. nov., Campylobacter concisus sp. nov., and Eikenella corrodens from Humans with Periodontal Disease. Int. J. Syst. Bacteriol. 1981, 31, 432–435. [Google Scholar]
  2. Lindblom, G.B.; Sjogren, E.; Hansson-Westerberg, J.; Kaijser, B. Campylobacter upsaliensis, C. sputorum sputorum and C. concisus as common causes of diarrhoea in Swedish children. Scand. J. Infect. Dis. 1995, 27, 187–188. [Google Scholar] [CrossRef]
  3. Aabenhus, R.; Permin, H.; On, S.L.; Andersen, L.P. Prevalence of Campylobacter concisus in diarrhoea of immunocompromised patients. Scand. J. Infect. Dis. 2002, 34, 248–252. [Google Scholar] [CrossRef] [Green Version]
  4. Nielsen, H.L.; Engberg, J.; Ejlertsen, T.; Bucker, R.; Nielsen, H. Short-term and medium-term clinical outcomes of Campylobacter concisus infection. Clin. Microbiol. Infect. 2012, 18, E459–E465. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  5. Nattramilarasu, P.K.; Bücker, R.; Lobo de Sa, F.D.; Fromm, A.; Nagel, O.; Lee, I.M.; Butkevych, E.; Mousavi, S.; Genger, C.; Klove, S.; et al. Campylobacter concisus Impairs Sodium Absorption in Colonic Epithelium via ENaC Dysfunction and Claudin-8 Disruption. Int. J. Mol. Sci. 2020, 21, 373. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  6. Barmeyer, C.; Erko, I.; Awad, K.; Fromm, A.; Bojarski, C.; Meissner, S.; Loddenkemper, C.; Kerick, M.; Siegmund, B.; Fromm, M.; et al. Epithelial barrier dysfunction in lymphocytic colitis through cytokine-dependent internalization of claudin-5 and -8. J. Gastroenterol. 2017, 52, 1090–1100. [Google Scholar] [CrossRef] [PubMed]
  7. Nielsen, H.L.; Dalager-Pedersen, M.; Nielsen, H. High risk of microscopic colitis after Campylobacter concisus infection: Population-based cohort study. Gut 2020, 69, 1952–1958. [Google Scholar] [CrossRef]
  8. Yde Aagaard, M.E.; Frahm Kirk, K.; Linde Nielsen, H.; Harder Tarpgaard, I.; Bach Hansen, J.; Nielsen, H. Campylobacter concisus is prevalent in the gastrointestinal tract of patients with microscopic colitis. Scand. J. Gastroenterol. 2020, 55, 924–930. [Google Scholar] [CrossRef]
  9. Barmeyer, C.; Fromm, M.; Schulzke, J.D. Active and passive involvement of claudins in the pathophysiology of intestinal inflammatory diseases. Pflugers Arch. 2017, 469, 15–26. [Google Scholar] [CrossRef]
  10. Chiba, H.; Osanai, M.; Murata, M.; Kojima, T.; Sawada, N. Transmembrane proteins of tight junctions. Biochim. Biophys. Acta 2008, 1778, 588–600. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  11. Otani, T.; Furuse, M. Tight Junction Structure and Function Revisited. Trends Cell Biol. 2020, 30, 805–817. [Google Scholar] [CrossRef]
  12. Tamura, A.; Hayashi, H.; Imasato, M.; Yamazaki, Y.; Hagiwara, A.; Wada, M.; Noda, T.; Watanabe, M.; Suzuki, Y.; Tsukita, S. Loss of claudin-15, but not claudin-2, causes Na+ deficiency and glucose malabsorption in mouse small intestine. Gastroenterology 2011, 140, 913–923. [Google Scholar] [CrossRef] [PubMed]
  13. Krug, S.M.; Gunzel, D.; Conrad, M.P.; Lee, I.F.; Amasheh, S.; Fromm, M.; Yu, A.S. Charge-selective claudin channels. Ann. N. Y. Acad. Sci. 2012, 1257, 20–28. [Google Scholar] [CrossRef]
  14. Krug, S.M.; Amasheh, S.; Richter, J.F.; Milatz, S.; Gunzel, D.; Westphal, J.K.; Huber, O.; Schulzke, J.D.; Fromm, M. Tricellulin forms a barrier to macromolecules in tricellular tight junctions without affecting ion permeability. Mol. Biol. Cell 2009, 20, 3713–3724. [Google Scholar] [CrossRef] [Green Version]
  15. Buschmann, M.M.; Shen, L.; Rajapakse, H.; Raleigh, D.R.; Wang, Y.; Wang, Y.; Lingaraju, A.; Zha, J.; Abbott, E.; McAuley, E.M.; et al. Occludin OCEL-domain interactions are required for maintenance and regulation of the tight junction barrier to macromolecular flux. Mol. Biol. Cell 2013, 24, 3056–3068. [Google Scholar] [CrossRef]
  16. Krug, S.M.; Schulzke, J.D.; Fromm, M. Tight junction, selective permeability, and related diseases. Semin. Cell Dev. Biol. 2014, 36, 166–176. [Google Scholar] [CrossRef]
  17. Staehlin, L.A. Further observations on the fine structure of freeze-cleaved tight junctions. J. Cell Sci. 1973, 13, 763–786. [Google Scholar]
  18. Walker, D.C.; MacKenzie, A.; Hulbert, W.C.; Hogg, J.C. A Re-assessment of the Tricellular Region of Epithelial Cell Tight Junctions in Trachea of Guinea Pig. Acta Anat. 1985, 122, 35–38. [Google Scholar] [CrossRef]
  19. Ikenouchi, J.; Furuse, M.; Furuse, K.; Sasaki, H.; Tsukita, S.; Tsukita, S. Tricellulin constitutes a novel barrier at tricellular contacts of epithelial cells. J. Cell Biol. 2005, 171, 939–945. [Google Scholar] [CrossRef] [PubMed]
  20. Ikenouchi, J.; Sasaki, H.; Tsukita, S.; Furuse, M.; Tsukita, S. Loss of occludin affects tricellular localization of tricellulin. Mol. Biol. Cell 2008, 19, 4687–4693. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  21. Pabst, R. The anatomical basis for the immune function of the gut. Anat. Emryol. 1987, 176, 135–144. [Google Scholar] [CrossRef]
  22. Biancone, L.; Monteleone, I.; Blanco, G.D.V.; Vavassori, P.; Pallone, F. Redident bacterial flora and immune system. Digest. Liver Dis. 2002, 34, S37–S43. [Google Scholar] [CrossRef]
  23. Zigmond, E.; Varol, C.; Farache, J.; Elmaliah, E.; Satpathy, A.T.; Friedlander, G.; Mack, M.; Shpigel, N.; Boneca, I.G.; Murphy, K.M.; et al. Ly6C hi monocytes in the inflamed colon give rise to proinflammatory effector cells and migratory antigen-presenting cells. Immunity 2012, 37, 1076–1090. [Google Scholar] [CrossRef] [Green Version]
  24. Bain, C.C.; Scott, C.L.; Uronen-Hansson, H.; Gudjonsson, S.; Jansson, O.; Grip, O.; Guilliams, M.; Malissen, B.; Agace, W.W.; Mowat, A.M. Resident and pro-inflammatory macrophages in the colon represent alternative context-dependent fates of the same Ly6Chi monocyte precursors. Mucosal. Immunol. 2013, 6, 498–510. [Google Scholar] [CrossRef]
  25. Raduolovic, K.; Mak’Anyengo, R.; Kaya, B.; Steinert, A.; Niess, J.H. Injections of Lipopolysaccharide into Mice to Mimic Entrance of Microbial-derived Products After Intestinal Barrier Breach. J. Vis. Exp. 2018. [Google Scholar] [CrossRef]
  26. Shi, Y.J.; Gong, H.F.; Zhao, Q.Q.; Liu, X.S.; Liu, C.; Wang, H. Critical role of toll-like receptor 4 (TLR4) in dextran sulfate sodium (DSS)-Induced intestinal injury and repair. Toxicol. Lett. 2019, 315, 23–30. [Google Scholar] [CrossRef] [PubMed]
  27. Lissner, D.; Schumann, M.; Batra, A.; Kredel, L.I.; Kuhl, A.A.; Erben, U.; May, C.; Schulzke, J.D.; Siegmund, B. Monocyte and M1 Macrophage-induced Barrier Defect Contributes to Chronic Intestinal Inflammation in IBD. Inflamm. Bowel Dis. 2015, 21, 1297–1305. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  28. Man, S.M.; Kaakoush, N.O.; Leach, S.T.; Nahidi, L.; Lu, H.K.; Norman, J.; Day, A.S.; Zhang, L.; Mitchell, H.M. Host attachment, invasion, and stimulation of proinflammatory cytokines by Campylobacter concisus and other non-Campylobacter jejuni Campylobacter species. J. Infect. Dis. 2010, 202, 1855–1865. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  29. Nielsen, H.L.; Nielsen, H.; Ejlertsen, T.; Engberg, J.; Gunzel, D.; Zeitz, M.; Hering, N.A.; Fromm, M.; Schulzke, J.D.; Bücker, R. Oral and fecal Campylobacter concisus strains perturb barrier function by apoptosis induction in HT-29/B6 intestinal epithelial cells. PLoS ONE 2011, 6, e23858. [Google Scholar] [CrossRef] [Green Version]
  30. Man, S.M.; Zhang, L.; Day, A.S.; Leach, S.T.; Lemberg, D.A.; Mitchell, H. Campylobacter concisus and other Campylobacter species in children with newly diagnosed Crohn’s disease. Inflamm. Bowel Dis. 2010, 16, 1008–1016. [Google Scholar] [CrossRef] [PubMed]
  31. Mukhopadhya, I.; Thomson, J.M.; Hansen, R.; Berry, S.H.; El-Omar, E.M.; Hold, G.L. Detection of Campylobacter concisus and other Campylobacter species in colonic biopsies from adults with ulcerative colitis. PLoS ONE 2011, 6, e21490. [Google Scholar] [CrossRef] [Green Version]
  32. Mahendran, V.; Riordan, S.M.; Grimm, M.C.; Tran, T.A.; Major, J.; Kaakoush, N.O.; Mitchell, H.; Zhang, L. Prevalence of Campylobacter species in adult Crohn’s disease and the preferential colonization sites of Campylobacter species in the human intestine. PLoS ONE 2011, 6, e25417. [Google Scholar] [CrossRef]
  33. Castano-Rodriguez, N.; Kaakoush, N.O.; Lee, W.S.; Mitchell, H.M. Dual role of Helicobacter and Campylobacter species in IBD: A systematic review and meta-analysis. Gut 2017, 66, 235–249. [Google Scholar] [CrossRef]
  34. Ismail, Y.; Mahendran, V.; Octavia, S.; Day, A.S.; Riordan, S.M.; Grimm, M.C.; Lan, R.; Lemberg, D.; Tran, T.A.; Zhang, L. Investigation of the enteric pathogenic potential of oral Campylobacter concisus strains isolated from patients with inflammatory bowel disease. PLoS ONE 2012, 7, e38217. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  35. Nielsen, H.L.; Nielsen, H.; Torpdahl, M. Multilocus sequence typing of Campylobacter concisus from Danish diarrheic patients. Gut Pathog. 2016, 8, 44. [Google Scholar] [CrossRef] [Green Version]
  36. Lobo de Sa, F.D.; Butkevych, E.; Nattramilarasu, P.K.; Fromm, A.; Mousavi, S.; Moos, V.; Golz, J.C.; Stingl, K.; Kittler, S.; Seinige, D.; et al. Curcumin Mitigates Immune-Induced Epithelial Barrier Dysfunction by Campylobacter jejuni. Int. J. Mol. Sci. 2019, 20, 4830. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  37. Butkevych, E.; Lobo de Sa, F.D.; Nattramilarasu, P.K.; Bücker, R. Contribution of Epithelial Apoptosis and Subepithelial Immune Responses in Campylobacter jejuni-Induced Barrier Disruption. Front. Microbiol. 2020, 11, 344. [Google Scholar] [CrossRef] [PubMed]
  38. Bücker, R.; Schulz, E.; Gunzel, D.; Bojarski, C.; Lee, I.F.; John, L.J.; Wiegand, S.; Janssen, T.; Wieler, L.H.; Dobrindt, U.; et al. alpha-Haemolysin of Escherichia coli in IBD: A potentiator of inflammatory activity in the colon. Gut 2014, 63, 1893–1901. [Google Scholar] [CrossRef]
  39. Bücker, R.; Krug, S.M.; Moos, V.; Bojarski, C.; Schweiger, M.R.; Kerick, M.; Fromm, A.; Janssen, S.; Fromm, M.; Hering, N.A.; et al. Campylobacter jejuni impairs sodium transport and epithelial barrier function via cytokine release in human colon. Mucosal. Immunol. 2018, 11, 474–485. [Google Scholar] [CrossRef] [Green Version]
  40. Kirk, K.F.; Meric, G.; Nielsen, H.L.; Pascoe, B.; Sheppard, S.K.; Thorlacius-Ussing, O.; Nielsen, H. Molecular epidemiology and comparative genomics of Campylobacter concisus strains from saliva, faeces and gut mucosal biopsies in inflammatory bowel disease. Sci. Rep. 2018, 8, 1902. [Google Scholar] [CrossRef]
  41. Kaakoush, N.O.; Deshpande, N.P.; Wilkins, M.R.; Tan, C.G.; Burgos-Portugal, J.A.; Raftery, M.J.; Day, A.S.; Lemberg, D.A.; Mitchell, H. The pathogenic potential of Campylobacter concisus strains associated with chronic intestinal diseases. PLoS ONE 2011, 6, e29045. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  42. Pulimood, A.B.; Ramakrishna, B.S.; Rita, A.B.; Srinivasan, P.; Mohan, V.; Gupta, S.; Perakath, B.; Kang, G.; Chandy, G.; Balasubramanian, K.A. Early activation of mucosal dendritic cells and macrophages in acute Campylobacter colitis and cholera: An in vivo study. J. Gastroenterol. Hepatol. 2008, 23, 752–758. [Google Scholar] [CrossRef]
  43. Heimesaat, M.M.; Genger, C.; Klove, S.; Weschka, D.; Mousavi, S.; Bereswill, S. The Host-Specific Intestinal Microbiota Composition Impacts Campylobacter coli Infection in a Clinical Mouse Model of Campylobacteriosis. Pathogens 2020, 9, 804. [Google Scholar] [CrossRef] [PubMed]
  44. Stockmann, M.; Schmitz, H.; Fromm, M.; Schmidt, W.; Pauli, G.; Scholz, P.; Riecken, E.O.; Schulzke, J.D. Mechanisms of Epithelial Barrier Impairment in HIV Infection. Ann. N. Y. Acad. Sci. 2000, 915, 293–303. [Google Scholar] [CrossRef]
  45. Shifflett, D.E.; Clayburgh, D.R.; Koutsouris, A.; Turner, J.R.; Hecht, G.A. Enteropathogenic, E. coli disrupts tight junction barrier function and structure in vivo. Lab. Investig. 2005, 85, 1308–1324. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  46. Ou, G.; Rompikuntal, P.K.; Bitar, A.; Lindmark, B.; Vaitkevicius, K.; Wai, S.N.; Hammarstrom, M.L. Vibrio cholerae cytolysin causes an inflammatory response in human intestinal epithelial cells that is modulated by the PrtV protease. PLoS ONE 2009, 4, e7806. [Google Scholar] [CrossRef]
  47. Nickerson, C.A.; Goodwin, T.J.; Terlonge, J.; Ott, C.M.; Buchanan, K.L.; Uicker, W.C.; Emami, K.; LeBlanc, C.L.; Ramamurthy, R.; Clarke, M.S.; et al. Three-dimensional tissue assemblies: Novel models for the study of Salmonella enterica serovar Typhimurium pathogenesis. Infect. Immun. 2001, 69, 7106–7120. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  48. Prasad, S.; Mingrino, R.; Kaukinen, K.; Hayes, K.L.; Powell, R.M.; MacDonald, T.T.; Collins, J.E. Inflammatory processes have differential effects on claudins 2, 3 and 4 in colonic epithelial cells. Lab. Investig. 2005, 85, 1139–1162. [Google Scholar] [CrossRef]
  49. Mankertz, J.; Amasheh, M.; Krug, S.M.; Fromm, A.; Amasheh, S.; Hillenbrand, B.; Tavalali, S.; Fromm, M.; Schulzke, J.D. TNFalpha up-regulates claudin-2 expression in epithelial HT-29/B6 cells via phosphatidylinositol-3-kinase signaling. Cell Tissue Res. 2009, 336, 67–77. [Google Scholar] [CrossRef]
  50. Zeissig, S.; Burgel, N.; Gunzel, D.; Richter, J.; Mankertz, J.; Wahnschaffe, U.; Kroesen, A.J.; Zeitz, M.; Fromm, M.; Schulzke, J.D. Changes in expression and distribution of claudin 2, 5 and 8 lead to discontinuous tight junctions and barrier dysfunction in active Crohn’s disease. Gut 2007, 56, 61–72. [Google Scholar] [CrossRef]
  51. Siegmund, B.; Lehr, H.A.; Fantuzzi, G.; Dinarello, C.A. IL-1 beta-converting enzyme (caspase-1) in intestinal inflammation. Proc. Natl. Acid. Sci. USA 2001, 98(23), 13249–13254. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  52. Jones, M.A.; Totemeyer, S.; Maskell, D.J.; Bryant, C.E.; Barrow, P.A. Induction of proinflammatory responses in the human monocytic cell line THP-1 by Campylobacter jejuni. Infect. Immun. 2003, 71, 2626–2633. [Google Scholar] [CrossRef] [Green Version]
  53. Sun, X.; Threadgill, D.; Jobin, C. Campylobacter jejuni induces colitis through activation of mammalian target of rapamycin signaling. Gastroenterology 2012, 142, 86–95.e85. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  54. Kaakoush, N.O.; Deshpande, N.P.; Man, S.M.; Burgos-Portugal, J.A.; Khattak, F.A.; Raftery, M.J.; Wilkins, M.R.; Mitchell, H.M. Transcriptomic and proteomic analyses reveal key innate immune signatures in the host response to the gastrointestinal pathogen Campylobacter concisus. Infect. Immun. 2015, 83, 832–845. [Google Scholar] [CrossRef] [Green Version]
  55. Krause, P.; Morris, V.; Greenbaum, J.A.; Park, Y.; Bjoerheden, U.; Mikulski, Z.; Muffley, T.; Shui, J.W.; Kim, G.; Cheroutre, H.; et al. IL-10-producing intestinal macrophages prevent excessive antibacterial innate immunity by limiting IL-23 synthesis. Nat. Commun. 2015, 6, 7055. [Google Scholar] [CrossRef]
  56. Kaakoush, N.O.; Mitchell, H.M.; Man, S.M. Role of emerging Campylobacter species in inflammatory bowel diseases. Inflamm. Bowel. Dis. 2014, 20, 2189–2197. [Google Scholar] [CrossRef]
  57. Kim, K.Y.; Oh, T.W.; Do, H.J.; Yang, J.H.; Yang, I.J.; Jeon, Y.H.; Go, Y.H.; Ahn, S.C.; Ma, J.Y.; Park, K.I. Acer palmatum thumb. Ethanol Extract Alleviates Interleukin-6-Induced Barrier Dysfunction and Dextran Sodium Sulfate-Induced Colitis by Improving Intestinal Barrier Function and Reducing Inflammation. J. Immunol. Res. 2018, 2018, 5718396. [Google Scholar] [CrossRef] [Green Version]
  58. Guo, S.; Gillingham, T.; Guo, Y.; Meng, D.; Zhu, W.; Walker, W.A.; Ganguli, K. Secretions of Bifidobacterium infantis and Lactobacillus acidophilus Protect Intestinal Epithelial Barrier Function. J. Pediatr. Gastroenterol. Nutr. 2017, 64, 404–412. [Google Scholar] [CrossRef]
  59. Ogawa, M.; Osada, H.; Hasegawa, A.; Ohno, H.; Yanuma, N.; Sasaki, K.; Shimoda, M.; Shirai, J.; Kondo, H.; Ohmori, K. Effect of interleukin-1beta on occludin mRNA expression in the duodenal and colonic mucosa of dogs with inflammatory bowel disease. J. Vet. Intern. Med. 2018, 32, 1019–1025. [Google Scholar] [CrossRef] [Green Version]
  60. Amasheh, M.; Grotjohann, I.; Amasheh, S.; Fromm, A.; Soderholm, J.D.; Zeitz, M.; Fromm, M.; Schulzke, J.D. Regulation of mucosal structure and barrier function in rat colon exposed to tumor necrosis factor alpha and interferon gamma in vitro: A novel model for studying the pathomechanisms of inflammatory bowel disease cytokines. Scand. J. Gastroenterol. 2009, 44, 1226–1235. [Google Scholar] [CrossRef] [PubMed]
  61. Hernandez-Chirlaque, C.; Aranda, C.J.; Ocon, B.; Capitan-Canadas, F.; Ortega-Gonzalez, M.; Carrero, J.J.; Suarez, M.D.; Zarzuelo, A.; Sanchez de Medina, F.; Martinez-Augustin, O. Germ-free and Antibiotic-treated Mice are Highly Susceptible to Epithelial Injury in DSS Colitis. J. Crohns. Colitis. 2016, 10, 1324–1335. [Google Scholar] [CrossRef] [Green Version]
  62. Fukumatsu, M.; Ogawa, M.; Arakawa, S.; Suzuki, M.; Nakayama, K.; Shimizu, S.; Kim, M.; Mimuro, H.; Sasakawa, C. Shigella targets epithelial tricellular junctions and uses a noncanonical clathrin-dependent endocytic pathway to spread between cells. Cell Host Microbe. 2012, 11, 325–336. [Google Scholar] [CrossRef] [Green Version]
  63. Morampudi, V.; Graef, F.A.; Stahl, M.; Dalwadi, U.; Conlin, V.S.; Huang, T.; Vallance, B.A.; Yu, H.B.; Jacobson, K. Tricellular Tight Junction Protein Tricellulin Is Targeted by the Enteropathogenic Escherichia coli Effector EspG1, Leading to Epithelial Barrier Disruption. Infect. Immun. 2017, 85. [Google Scholar] [CrossRef] [Green Version]
  64. Gemmell, M.R.; Berry, S.; Mukhopadhya, I.; Hansen, R.; Nielsen, H.L.; Bajaj-Elliott, M.; Nielsen, H.; Hold, G.L. Comparative genomics of Campylobacter concisus: Analysis of clinical strains reveals genome diversity and pathogenic potential. Emerg. Microbes. Infect. 2018, 7, 116. [Google Scholar] [CrossRef] [Green Version]
  65. Liu, F.; Chen, S.; Luu, L.D.W.; Lee, S.A.; Tay, A.C.Y.; Wu, R.; Riordan, S.M.; Lan, R.; Liu, L.; Zhang, L. Analysis of complete Campylobacter concisus genomes identifies genomospecies features, secretion systems and novel plasmids and their association with severe ulcerative colitis. Microb. Genom. 2020, 6. [Google Scholar] [CrossRef]
  66. Krug, S.M.; Bojarski, C.; Fromm, A.; Lee, I.M.; Dames, P.; Richter, J.F.; Turner, J.R.; Fromm, M.; Schulzke, J.D. Tricellulin is regulated via interleukin-13-receptor alpha2, affects macromolecule uptake, and is decreased in ulcerative colitis. Mucosal. Immunol. 2018, 11, 345–356. [Google Scholar] [CrossRef] [Green Version]
  67. Keita, A.V.; Lindqvist, C.M.; Ost, A.; Magana, C.D.L.; Schoultz, I.; Halfvarson, J. Gut Barrier Dysfunction-A Primary Defect in Twins with Crohn’s Disease Predominantly Caused by Genetic Predisposition. J. Crohns. Colitis. 2018, 12, 1200–1209. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  68. Epple, H.J.; Friebel, J.; Moos, V.; Troeger, H.; Krug, S.M.; Allers, K.; Schinnerling, K.; Fromm, A.; Siegmund, B.; Fromm, M.; et al. Architectural and functional alterations of the small intestinal mucosa in classical Whipple’s disease. Mucosal. Immunol. 2017, 10, 1542–1552. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  69. Bucker, R.; Krug, S.M.; Fromm, A.; Nielsen, H.L.; Fromm, M.; Nielsen, H.; Schulzke, J.D. Campylobacter fetus impairs barrier function in HT-29/B6 cells through focal tight junction alterations and leaks. Ann. N. Y. Acad. Sci. 2017, 1405, 189–201. [Google Scholar] [CrossRef]
  70. Amasheh, M.; Fromm, A.; Krug, S.M.; Amasheh, S.; Andres, S.; Zeitz, M.; Fromm, M.; Schulzke, J.D. TNFalpha-induced and berberine-antagonized tight junction barrier impairment via tyrosine kinase, Akt and NFkappaB signaling. J. Cell Sci. 2010, 123, 4145–4155. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  71. Bergmann, S.; von Buenau, B.; Vidal, Y.S.S.; Haftek, M.; Wladykowski, E.; Houdek, P.; Lezius, S.; Duplan, H.; Basler, K.; Dahnhardt-Pfeiffer, S.; et al. Claudin-1 decrease impacts epidermal barrier function in atopic dermatitis lesions dose-dependently. Sci. Rep. 2020, 10, 2024. [Google Scholar] [CrossRef] [PubMed]
  72. Weber, C.R.; Nalle, S.C.; Tretiakova, M.; Rubin, D.T.; Turner, J.R. Claudin-1 and claudin-2 expression is elevated in inflammatory bowel disease and may contribute to early neoplastic transformation. Lab. Investig. 2008, 88, 1110–1120. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  73. Pope, J.L.; Ahmed, R.; Bhat, A.A.; Washington, M.K.; Singh, A.B.; Dhawan, P. Claudin-1 overexpression in intestinal epithelial cells enhances susceptibility to adenomatous polyposis coli-mediated colon tumorigenesis. Mol. Cancer 2014, 13, 167. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  74. Troeger, H.; Epple, H.J.; Schneider, T.; Wahnschaffe, U.; Ullrich, R.; Burchard, G.D.; Jelinek, T.; Zeitz, M.; Fromm, M.; Schulzke, J.D. Effect of chronic Giardia lamblia infection on epithelial transport and barrier function in human duodenum. Gut 2007, 56, 328–335. [Google Scholar] [CrossRef] [PubMed]
Ijms 22 02043 g001 550
Figure 1. Transepithelial electrical resistance (TER) of Campylobacter concisus-infected colonic epithelial cell monolayers in monoculture and co-culture (with M1-macrophage-like THP-1 cells on the basal side) conditions. (A) Changes in TER in uninfected controls (white/bright grey bars) in monoculture and co-culture were recorded and compared with C. concisus-infections (dark grey/black bars) at 0 h (before infection), 24 h and 48 h post-C. concisus infection with a multiplicity of infection (MOI) of 200 (n = 23 in three independent experiments, ns = not significant, *** p < 0.001).
Figure 1. Transepithelial electrical resistance (TER) of Campylobacter concisus-infected colonic epithelial cell monolayers in monoculture and co-culture (with M1-macrophage-like THP-1 cells on the basal side) conditions. (A) Changes in TER in uninfected controls (white/bright grey bars) in monoculture and co-culture were recorded and compared with C. concisus-infections (dark grey/black bars) at 0 h (before infection), 24 h and 48 h post-C. concisus infection with a multiplicity of infection (MOI) of 200 (n = 23 in three independent experiments, ns = not significant, *** p < 0.001).
Ijms 22 02043 g001
Ijms 22 02043 g002 550
Figure 2. Changes in tight junction protein expression in HT-29/B6-GR/MR cell monolayers in monoculture or co-culture condition (with M1-macrophage-like THP-1 cells on the basal side) 48 h after Campylobacter concisus infection. Western blots and densitometry of claudins (claudin-1, -2, -4, -5, -7, -8), occludin and tricellulin (n = 6–9 in two to three independent experiments, ns = not significant, ** p < 0.01, *** p < 0.001).
Figure 2. Changes in tight junction protein expression in HT-29/B6-GR/MR cell monolayers in monoculture or co-culture condition (with M1-macrophage-like THP-1 cells on the basal side) 48 h after Campylobacter concisus infection. Western blots and densitometry of claudins (claudin-1, -2, -4, -5, -7, -8), occludin and tricellulin (n = 6–9 in two to three independent experiments, ns = not significant, ** p < 0.01, *** p < 0.001).
Ijms 22 02043 g002
Ijms 22 02043 g003 550
Figure 3. Subcellular distribution of occludin in Campylobacter concisus-infected HT-29/B6-GR/MR cell monolayers 48 h post infection. Occludin (green) and ZO-1 (red) are co-localized in the bicellular tight junction (bTJ) of controls in both monoculture (HT-29/B6-GR/MR cell monolayers) and co-culture conditions (HT-29/B6-GR/MR cell monolayers with M1-macrophage-like THP-1 cells on the basal side). Nuclei were stained blue with DAPI. In C. concisus-infected cell monolayers in monoculture and co-culture condition, white arrows indicate redistribution of occludin from bTJ into intracellular compartments.
Figure 3. Subcellular distribution of occludin in Campylobacter concisus-infected HT-29/B6-GR/MR cell monolayers 48 h post infection. Occludin (green) and ZO-1 (red) are co-localized in the bicellular tight junction (bTJ) of controls in both monoculture (HT-29/B6-GR/MR cell monolayers) and co-culture conditions (HT-29/B6-GR/MR cell monolayers with M1-macrophage-like THP-1 cells on the basal side). Nuclei were stained blue with DAPI. In C. concisus-infected cell monolayers in monoculture and co-culture condition, white arrows indicate redistribution of occludin from bTJ into intracellular compartments.
Ijms 22 02043 g003
Ijms 22 02043 g004 550
Figure 4. Subcellular distribution of tricellulin in Campylobacter concisus-infected HT-29/B6-GR/MR cell monolayers 48 h post infection. Tricellulin (green) is localized in the tricellular tight junction (tTJ) along with ZO-1 (red) in controls of both monoculture (HT-29/B6-GR/MR cell monolayers) and co-culture (HT-29/B6-GR/MR cell monolayers with M1-macrophage-like THP-1 cells on the basal side). Nuclei (blue) were stained by DAPI. In C. concisus-infected cell monolayers in monoculture and co-culture condition, white arrows indicate redistribution of tricellulin from the tTJ domain into intracellular compartments.
Figure 4. Subcellular distribution of tricellulin in Campylobacter concisus-infected HT-29/B6-GR/MR cell monolayers 48 h post infection. Tricellulin (green) is localized in the tricellular tight junction (tTJ) along with ZO-1 (red) in controls of both monoculture (HT-29/B6-GR/MR cell monolayers) and co-culture (HT-29/B6-GR/MR cell monolayers with M1-macrophage-like THP-1 cells on the basal side). Nuclei (blue) were stained by DAPI. In C. concisus-infected cell monolayers in monoculture and co-culture condition, white arrows indicate redistribution of tricellulin from the tTJ domain into intracellular compartments.
Ijms 22 02043 g004
Ijms 22 02043 g005 550
Figure 5. Epithelial permeability for fluorescein (332 Da) and FITC-dextran (4 kDa) in HT-29/B6-GR/MR cell monolayers under monoculture or co-culture conditions (with M1-macrophage-like THP-1 cells on the basal side) after Campylobacter concisus infection. (A) Permeability for fluorescein in monoculture and co-culture conditions 48 h after C. concisus infection (n = 6–8 in three independent experiments, ns = not significant, ** p < 0.01). (B) Permeability for 4 kDa FITC-dextran in monoculture or co-culture 48 h after C. concisus infection (n = 6–8 in three independent experiments, ns = not significant, ** p < 0.01).
Figure 5. Epithelial permeability for fluorescein (332 Da) and FITC-dextran (4 kDa) in HT-29/B6-GR/MR cell monolayers under monoculture or co-culture conditions (with M1-macrophage-like THP-1 cells on the basal side) after Campylobacter concisus infection. (A) Permeability for fluorescein in monoculture and co-culture conditions 48 h after C. concisus infection (n = 6–8 in three independent experiments, ns = not significant, ** p < 0.01). (B) Permeability for 4 kDa FITC-dextran in monoculture or co-culture 48 h after C. concisus infection (n = 6–8 in three independent experiments, ns = not significant, ** p < 0.01).
Ijms 22 02043 g005
Ijms 22 02043 g006 550
Figure 6. Epithelial apoptosis in HT-29/B6-GR/MR cell monolayers in monoculture or co-culture conditions (with M1-macrophage-like THP-1 cells on the basal side) after Campylobacter concisus infection. (A) The apoptotic cells in HT-29/B6-GR/MR cell monolayers were stained green by TUNEL assay kit and nuclei were stained blue by DAPI. (B) Percentage of apoptotic cells (TUNEL-positive) cells, quantified in five to eight randomly picked regions of the cell monolayers (n = 3 in independent experiments, ns = not significant, ** p < 0.01, *** p < 0.001).
Figure 6. Epithelial apoptosis in HT-29/B6-GR/MR cell monolayers in monoculture or co-culture conditions (with M1-macrophage-like THP-1 cells on the basal side) after Campylobacter concisus infection. (A) The apoptotic cells in HT-29/B6-GR/MR cell monolayers were stained green by TUNEL assay kit and nuclei were stained blue by DAPI. (B) Percentage of apoptotic cells (TUNEL-positive) cells, quantified in five to eight randomly picked regions of the cell monolayers (n = 3 in independent experiments, ns = not significant, ** p < 0.01, *** p < 0.001).
Ijms 22 02043 g006
Ijms 22 02043 g007 550
Figure 7. Cytokines released from M1-macrophage-like THP-1 cells in co-culture with cell monolayers. Under co-culture condition (HT-29/B6-GR/MR cell monolayers with M1-macrophage-like THP-1 cells on the basal side), the cytokines released from THP-1 cells were quantified. The cytokines TNF-α, IL-1β, IL-6, and IL-10 released from the infected cells (black bars) were compared to cytokines released from uninfected controls (bright grey bars) (n = 6 in two independent experiments, *** p < 0.001). The cell monolayers were infected with C. concisus at MOI of 200 on both apical and basal side.
Figure 7. Cytokines released from M1-macrophage-like THP-1 cells in co-culture with cell monolayers. Under co-culture condition (HT-29/B6-GR/MR cell monolayers with M1-macrophage-like THP-1 cells on the basal side), the cytokines released from THP-1 cells were quantified. The cytokines TNF-α, IL-1β, IL-6, and IL-10 released from the infected cells (black bars) were compared to cytokines released from uninfected controls (bright grey bars) (n = 6 in two independent experiments, *** p < 0.001). The cell monolayers were infected with C. concisus at MOI of 200 on both apical and basal side.
Ijms 22 02043 g007
Ijms 22 02043 g008 550
Figure 8. Cell viability in Campylobacter concisus-infected HT-29/B6-GR/MR cell monolayers. Absorbance values recorded at 450 nm with a reference wavelength of 600 nm (WST-8 in the CCK-8 assay) in controls and C. concisus-infected cell monolayers 48 h post infection (n = 6 in two independent experiments, ns = not significant). The cell monolayers in mono-culture (HT-29/B6-GR/MR) and co-culture conditions (THP-1 cells on the basal side) were infected with C. concisus at MOI of 200 on both apical and basal side.
Figure 8. Cell viability in Campylobacter concisus-infected HT-29/B6-GR/MR cell monolayers. Absorbance values recorded at 450 nm with a reference wavelength of 600 nm (WST-8 in the CCK-8 assay) in controls and C. concisus-infected cell monolayers 48 h post infection (n = 6 in two independent experiments, ns = not significant). The cell monolayers in mono-culture (HT-29/B6-GR/MR) and co-culture conditions (THP-1 cells on the basal side) were infected with C. concisus at MOI of 200 on both apical and basal side.
Ijms 22 02043 g008
Ijms 22 02043 g009 550
Figure 9. Leaky gut cell model to investigate colonic epithelial barrier dysfunction induced by Campylobacter concisus.
Figure 9. Leaky gut cell model to investigate colonic epithelial barrier dysfunction induced by Campylobacter concisus.
Ijms 22 02043 g009
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Nattramilarasu, P.K.; Lobo de Sá, F.D.; Schulzke, J.-D.; Bücker, R. Immune-Mediated Aggravation of the Campylobacter concisus-Induced Epithelial Barrier Dysfunction. Int. J. Mol. Sci. 2021, 22, 2043. https://doi.org/10.3390/ijms22042043

AMA Style

Nattramilarasu PK, Lobo de Sá FD, Schulzke J-D, Bücker R. Immune-Mediated Aggravation of the Campylobacter concisus-Induced Epithelial Barrier Dysfunction. International Journal of Molecular Sciences. 2021; 22(4):2043. https://doi.org/10.3390/ijms22042043

Chicago/Turabian Style

Nattramilarasu, Praveen Kumar, Fábia Daniela Lobo de Sá, Jörg-Dieter Schulzke, and Roland Bücker. 2021. "Immune-Mediated Aggravation of the Campylobacter concisus-Induced Epithelial Barrier Dysfunction" International Journal of Molecular Sciences 22, no. 4: 2043. https://doi.org/10.3390/ijms22042043

APA Style

Nattramilarasu, P. K., Lobo de Sá, F. D., Schulzke, J. -D., & Bücker, R. (2021). Immune-Mediated Aggravation of the Campylobacter concisus-Induced Epithelial Barrier Dysfunction. International Journal of Molecular Sciences, 22(4), 2043. https://doi.org/10.3390/ijms22042043

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop