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Article

Recombinant IGF-1 Induces Sex-Specific Changes in Bone Composition and Remodeling in Adult Mice with Pappa2 Deficiency

by
Leticia Rubio
1,†,
Antonio Vargas
2,†,
Patricia Rivera
2,
Antonio J. López-Gambero
2,
Rubén Tovar
2,
Julian K. Christians
3,
Stella Martín-de-las-Heras
1,
Fernando Rodríguez de Fonseca
2,
Julie A. Chowen
4,5,6,
Jesús Argente
4,5,6,7,* and
Juan Suárez
1,2,*
1
Departamento de Anatomía Humana, Medicina Legal e Historia de la Ciencia, Instituto de Investigación Biomédica de Málaga (IBIMA), Universidad de Málaga, 29071 Málaga, Spain
2
Unidad de Gestión Clínica de Salud Mental, IBIMA, Hospital Regional Universitario de Málaga, 29010 Málaga, Spain
3
Department of Biological Sciences, Simon Fraser University, Burnaby, BC V5A 1S6, Canada
4
Department of Endocrinology, Instituto de Investigación Biomédica la Princesa, Hospital Infantil Universitario Niño Jesús, 28009 Madrid, Spain
5
Centro de Investigación Biomédica en Red Fisiología de la Obesidad y Nutrición (CIBEROBN), 28029 Madrid, Spain
6
IMDEA Food Institute, Campus of International Excellence (CEI) UAM + CSIC, 28049 Madrid, Spain
7
Department of Pediatrics, Universidad Autónoma de Madrid, 28029 Madrid, Spain
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2021, 22(8), 4048; https://doi.org/10.3390/ijms22084048
Submission received: 16 March 2021 / Revised: 4 April 2021 / Accepted: 11 April 2021 / Published: 14 April 2021
(This article belongs to the Special Issue Regulation of Bone Mineral Homeostasis)
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Abstract

:
Deficiency of pregnancy-associated plasma protein-A2 (PAPP-A2), an IGF-1 availability regulator, causes postnatal growth failure and dysregulation of bone size and density. The present study aimed to determine the effects of recombinant murine IGF-1 (rmIGF-1) on bone composition and remodeling in constitutive Pappa2 knock-out (ko/ko) mice. To address this challenge, X-ray diffraction (XRD), attenuated total reflection-fourier transform infra-red (ATR-FTIR) spectroscopy and gene expression analysis of members of the IGF-1 system and bone resorption/formation were performed. Pappa2ko/ko mice (both sexes) had reduced body and bone length. Male Pappa2ko/ko mice had specific alterations in bone composition (mineral-to-matrix ratio, carbonate substitution and mineral crystallinity), but not in bone remodeling. In contrast, decreases in collagen maturity and increases in Igfbp3, osteopontin (resorption) and osteocalcin (formation) characterized the bone of Pappa2ko/ko females. A single rmIGF-1 administration (0.3 mg/kg) induced short-term changes in bone composition in Pappa2ko/ko mice (both sexes). rmIGF-1 treatment in Pappa2ko/ko females also increased collagen maturity, and Igfbp3, Igfbp5, Col1a1 and osteopontin expression. In summary, acute IGF-1 treatment modifies bone composition and local IGF-1 response to bone remodeling in mice with Pappa2 deficiency. These effects depend on sex and provide important insights into potential IGF-1 therapy for growth failure and bone loss and repair.

1. Introduction

The growth hormone (GH)/insulin-like growth factor 1 (IGF-1) signaling system constitutes a pleotropic axis required for bone development, mineral deposition and skeletal growth [1,2,3,4,5]. IGF-1 exerts its effects on skeletal growth and metabolism by interacting with other bone regulators like thyroid and parathyroid hormones and sex steroids, among others [6,7,8]. The rise in circulating levels of GH and IGF-1 during puberty is associated with peak bone acquisition, while their declining levels during aging are associated with bone loss. Molecular mechanisms of bone mass homeostasis require a balance between bone resorption by osteoclasts and bone formation by osteoblasts [9]. A reduction of IGF-1 signaling by ablation, inactivation or haploinsufficiency in mouse models causes severe growth retardation in a sex and age-dependent manner [3,10,11,12,13]. Clinically, mutations in IGF-1 and IGF-1 receptor also result in intrauterine and postnatal growth retardation [14,15].
Pregnancy-associated plasma protein-A2 (PAPP-A2 or pappalysin-2) is a highly specific metalloprotease of IGF binding proteins 3 and 5 (IGFBP-3 and IGFBP-5) that regulates the dissociation of IGF-1 from secondary and ternary complexes that include IGF acid-labile subunit (IGF-ALS) [5,16,17,18,19,20,21]. Biologically-available IGF-1 binds its target receptors and activates intracellular signaling pathways and gene expression to regulate growth physiology and bone metabolism [5,22,23].
A new syndrome characterized by short stature, skeletal abnormalities and reduced bone density was discovered by Argente and Dauber in 2016 and was found to be caused by loss-of-function mutations in the human PAPP-A2 gene [24,25,26,27]. PAPP-A2-deficient patients exhibit high circulating levels of IGF-1 bound to its ternary complex (IGFBP-3 or IGFBP-5 and IGF-ALS) resulting in decreased concentrations of free IGF-1 [24]. As these patients do not exhibit GH deficiency, and no PAPP-A2 replacement therapy is available, they were treated with recombinant human IGF-1 (rhIGF-1). Short-term therapy with rhIGF-1 in children with PAPP-A2 deficiency increased growth velocity and height [28], and improved bone mineral density and trabecular bone structure [29].
Recent studies in animal models with Pappa2 gene deletion point to the mechanisms by which PAPP-A2 contributes to skeletal growth and bone mineral density [30,31,32,33]. In Pappa2-deficient mice, changes in trabecular and cortical mineral density were associated with increased circulating levels of IGFBP-5 and reduced circulating levels of factors related to bone turnover [33].
In the present study, we used an animal model of Pappa2 gene deletion (Pappa2ko/ko) with a reduction in skeletal growth and bone mineral density, as previously described [29,31]. We explored the hypothesis that Pappa2 deletion impairs bone properties through changes in crystallinity related to biological hydroxyapatite-(CaOH) and parameters of bone composition calculated according to relative contents of phosphates, carbonates and amides. We also examined the short-term effects of a single injection of recombinant murine IGF-1 (rmIGF-1) on bone composition. Toward this goal, we employed two physical-chemical approaches: (1) X-ray powder diffraction (XRD) and Rietveld refinement for the quantitative analysis of crystallographic parameters, associated with biological hydroxyapatite-(CaOH); and (2) attenuated total reflection-fourier transform infra-red (ATR-FTIR) spectroscopy for the quantification of relevant parameters that reflect the relative content of bone compounds containing phosphates (v1v3PO43−), carbonates (v2CO32−) and amides I (vC=O), such as mineral-to-matrix ratio, carbonate substitution, mineral crystallinity and collagen maturity in bone. In addition, we aimed to assess whether changes in bone composition are associated with a local IGF-1 response to bone remodeling. Toward this goal, we employed real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) to analyze the gene expression of the local IGF-1 system (Igfbp3, Igfbp5, Igfals) and bone resorption/formation markers (Col1a1, osteopontin, osteocalcin), and the response to rmIGF-1 treatment in bone.

2. Results

2.1. Pappa2 Deletion Reduces Body and Bone Length

There were overall effects of genotype and sex on the body length of adult mice (Table 1). Tukey analysis indicated that Pappa2ko/ko males and females were shorter than the respective Pappa2wt/wt males (### p < 0.001) and females (*** p < 0.001). The body length of Pappa2ko/ko females was less than that of Pappa2ko/ko males (&& p < 0.01; Table 1).
Overall, the effects of genotype on the femur and tibia length, femur and tibia weight, and relative weights of tibia were found (Table 1). The overall effects of sex on the femur length and weight, tibia weight and relative weights of femur and tibia were also observed (Table 1), with females having overall lower values than males (### p < 0.001). Significant interactions between genotype and sex were found in femur length and weight, tibia weight and femur/body weight ratio of the adult mice (Table 1), with males showing greater effects of Pappa2 deletion (#/### p < 0.05/0.001) than females (*** p < 0.001).

2.2. Pappa2 Deletion Alters Hydroxyapatite Crystallinity in the Femur of Male Mice, But Not Female Mice

Crystallographic indexes of hydroxyapatite-(CaOH) found in the femurs of adult mice were quantified by XRD and Rietveld refinement. The overall effects of genotype on hydroxyapatite crystallinity and crystallite size were found (Table 2). Sex effects on hydroxyapatite crystallinity were also observed, with females having a higher index than males (## p < 0.01). A significant interaction between genotype and sex was found in hydroxyapatite crystallinity (Table 2), with a significant increase in Pappa2ko/ko males compared with Pappa2wt/wt males (## p < 0.01). No difference between WT (wild-type) and KO (knock-out) females was found. Crystallite size was significantly lower in Pappa2ko/ko mice (# p < 0.05). No interactions between factors were observed for crystallite size (Table 2) or other parameters such as R-Bragg factor, cell volume, crystal linear absorbance coefficient or crystal density (Table S1).

2.3. Pappa2 Deletion Alters Bone Composition in the Femur of Male Mice, but Not Female Mice

Relative contents of compounds containing phosphates (v1v3PO43−), carbonates (v2CO32−) and amides I (vC=O) in the femurs of adult mice were identified by ATR-FTIR spectroscopy, and quantified to calculate relevant parameters related to bone composition, such as mineral-to-organic matrix (M/M) ratio, an index of the relative amount of phosphate per amount of collagen; carbonate substitution (C/p ratio), an index of phosphate-to-carbonate-substituted apatites; mineral crystallinity, a degree of order in a solid; and collagen crosslink ratio, an index of collagen maturity. The overall effects of genotype on M/M ratio, C/P ratio, mineral crystallinity and collagen maturity were found (Table 3), with Pappa2 deletion inducing a higher M/M ratio (# p < 0.05 in males) and lower C/P ratio (## p < 0.01 in males), mineral crystallinity (# p < 0.05 in males) and collagen maturity (##/&&& p < 0.01/0.001 in both sexes) than in respective WT mice. The overall effects of sex on the M/M ratio, C/P ratio and collagen maturity were observed (Table 3), with females having an overall higher M/M ratio (# p < 0.05) and lower C/P ratio (# p < 0.05) and collagen maturity (### p < 0.001) than males. Interestingly, significant interactions between genotype and sex were found in mineral crystallinity, and reflected significant decreases in Pappa2ko/ko males compared with controls (# p < 0.05) and no significant effect of Pappa2 deletion in females (Table 3).

2.4. rmIGF-1 Induces Sex-Specific Changes in Bone Composition of Pappa2ko/ko Mice

Short-term effects of rmIGF-1 on bone composition were also evaluated over time (0, 30, 120 and 240 min) in male and female femurs (Figure 1). The overall effects of genotype, sex and time on M/M ratio, C/P ratio, mineral crystallinity and collagen maturity were detected (F1.114 > 18.67, p < 0.001). Significant interactions between genotype, sex and time were found in M/M ratio, C/P ratio, mineral crystallinity and collagen maturity (F3.114 = 3.50, p = 0.02; F3.114 = 4.04, p = 0.009; F3.114 = 5.58, p = 0.001; F3.114 = 30.85, p = 0.001, respectively), suggesting that rmIGF-1 treatment affected bone composition depending on sex and genotype over time.
In males, treatment with rmIGF-1 induced an acute, transitory increase in the M/M ratio of the Pappa2wt/wt femur (30′ versus 0′: *** p < 0.001), which gradually returned to normality over time (120′ and 240′ versus 0′: * p < 0.05/ns respectively; Figure 1A). However, this transitory increase was not observed in the M/M ratio of the Pappa2ko/ko male femur. Treatment with rmIGF-1 in Pappa2ko/ko males induced a gradual increase in the M/M ratio over time (30′, 120′ and 240′ versus 0′: */** p < 0.05/0.01; Figure 1A). In contrast, treatment with rmIGF-1 induced opposite effects on C/P ratio, mineral crystallinity and collagen maturity in the femur of Pappa2wt/wt males. Acute decreases were found in the C/P ratio, mineral crystallinity and collagen maturity in the Pappa2wt/wt male femur (30′ versus 0′: *** p < 0.001; Figure 1B–D), and while C/P ratio and collagen maturity returned to normality over time (120′ and 240′ versus 0′: * p < 0.05/ns and ** p < 0.01/ns respectively; Figure 1B,D), mineral crystallinity remained low (120′ and 240′ versus 0′: *** p < 0.001; Figure 1C). Treatment with rmIGF-1 in Pappa2ko/ko males induced gradual decreases in the C/P ratio and mineral crystallinity over time (30′, 120′ and 240′ versus 0′: */** p < 0.05/0.01; Figure 1B,C) and no changes were found in collagen maturity (Figure 1D). Basal differences between genotypes (Pappa2ko/ko versus Pappa2wt/wt males at 0′: #/## p < 0.05/0.01) observed in M/M ratio, C/P ratio and mineral crystallinity were not found 30′ after rmIGF-1 treatment. Excepting mineral crystallinity, differences between genotypes were gradually recovered over time (120′: ##p < 0.01; 240′: ### p < 0.001) after rmIGF-1 treatment (Figure 1B–D).
In females (Figure 1E–H), rmIGF-1 treatment increased the M/M ratio and decreased the C/P ratio and mineral crystallinity in both genotypes, effects that were maintained over time (30′, 120′ and 240′ versus 0′: */**/*** p < 0.05/0.01/0.001). These rmIGF-1-induced changes were more significant in the femur of Pappa2ko/ko females (Figure 1E–G). However, treatment with rmIGF-1 did not change collagen maturity of the Pappa2wt/wt female femur, while a transitory increase in the collagen maturity was found in the Pappa2ko/ko female femur (30′ and 120′ versus 0′: *** p < 0.001) that gradually returned to normality over time (240′ versus 0′: ns; Figure 1H). Accordingly, significant differences between genotypes (Pappa2ko/ko versus Pappa2wt/wt females: #/##/### p < 0.05/0.01/0.001) were found in M/M ratio, C/P ratio and mineral crystallinity after rmIGF-1 treatment and mostly maintained over time (Figure 1E–G).

2.5. Pappa2 Deletion Affects Igfbp3 Gene Expression in the Tibia

To further understand the effects of Pappa2 gene deletion and the putative low IGF bioavailability on bone composition we evaluated the local IGF-1 system by analyzing relative mRNA levels of Pappa2, Igfbp3, Igfbp4, Igfbp5, Igfals and Stc2 in the tibia (Table 4). No signal was detected for mRNA levels of Pappa2, Igfbp4 or Stc2 in the tibias of either sex. An overall effect of genotype on the mRNA levels of Igfbp3 was observed (Table 4), with Pappa2 deletion inducing higher expression than WT (Pappa2ko/ko versus Pappa2wt/wt females: ** p < 0.01). The overall effects of sex on the mRNA levels of Igfbp5 and Igfals were found, with females having lower expression than males (Pappa2wt/wt females versus Pappa2wt/wt males: ## p < 0.01; Pappa2ko/ko females vs. Pappa2kot/ko males: && p < 0.01). No interactions between genotype and sex were found in the mRNA levels of Igfbp3, Igfbp5 or Igfals (Table 4).

2.6. rmIGF-1 Induces Sex-Specific Changes in Local IGF-1 System of Pappa2ko/ko Mice

We analyzed the short-term effects of rmIGF-1 treatment on the relative mRNA levels of Igfbp3, Igfbp5 and Igfals over time (0, 30, 120 and 240 min) in the male and female tibias (Figure 2). Overall effects of genotype on Igfbp3 and Igfbp5 (F1.114 > 48.12, p < 0.0001), and overall effects of sex (F1.114 > 33.76, p < 0.0001) and time (F1.114 > 3.37, p < 0.024) on all three factors were found. Significant interactions between genotype, sex and time were observed in the mRNA levels of Igfbp3, Igfbp5 and Igfals (F3.114 = 3.92, p = 0.011; F3.114 = 5.39, p = 0.002; F3.114 = 3.33, p = 0.022 respectively), suggesting that rmIGF-1 treatment affected local IGF-1 system depending on sex and genotype over time.
In males, treatment with rmIGF-1 did not modify the mRNA levels of Igfbp3 in the Pappa2wt/wt tibia (Figure 2A). However, Igfbp3 mRNA levels were significantly increased over time in the Pappa2ko/ko tibia (240′ versus 0′: *** p < 0.001). rmIGF-1 treatment decreased the mRNA levels of Igfbp5 and Igfals in male tibia of both genotypes (30′, 120′ and 240′ versus 0′: */**/*** p < 0.05/0.01/0.001; Figure 2B,C). rmIGF-1 treatment-related differences between genotypes (Pappa2ko/ko versus Pappa2wt/wt males) were observed in the tibia mRNA levels of Igfbp3 (120′ and 240′: ##/### p < 0.01/0.001; Figure 2A) and Igfals (120′: # p < 0.05; Figure 2C).
In females, treatment with rmIGF-1 did not modify the mRNA levels of Igfbp3 or Igfbp5 in the Pappa2wt/wt tibia (Figure 2D,E). However, Igfbp3 and Igfbp5 mRNA levels were significantly increased over time in the Pappa2ko/ko tibia (30′, 120′ and 240′ versus 0′: */**/*** p < 0.05/0.01/0.001). Treatment with rmIGF-1 induced a transitory increase in the Igfals mRNA levels of the Pappa2wt/wt female tibia (30′ and 120′ versus 0′: * p < 0.05), which gradually returned to normality over time (Figure 2E). In contrast, treatment with rmIGF-1 did not modify Igfals mRNA levels of the Pappa2ko/ko female tibia (Figure 2E). Accordingly, rmIGF-1 treatment-related differences between genotypes (Pappa2ko/ko versus Pappa2wt/wt females) were observed in the mRNA levels of Igfbp3, Igfbp5 and Igfals in the tibia over time (30′: ## p < 0.01; 120′ and 240′: ### p < 0.001; Figure 2D–F).

2.7. Pappa2 Deletion Affects the Gene Expression of Bone Remodeling Markers in the Tibia

To further understand the effects of Pappa2 deletion and local IGF-1 system dysregulation on bone composition, we evaluated bone turnover or remodeling (resorption/formation) by analyzing relative mRNA expression of osteopontin, osteocalcin and Col1a1 in the tibia (Table 5). The overall effects of the genotype on the mRNA levels of osteocalcin (bone resorption) were found, with Pappa2 deficient mice having overall higher expression than WT (Table 5). Significant interactions between genotype and sex were found on the mRNA levels of osteopontin and osteocalcin (bone formation), and reflected significant increases in Pappa2ko/ko females compared with controls (*/**p < 0.05/0.01).

2.8. rmIGF-1 Induces Sex-Specific Changes in Bone Remodeling Markers of Pappa2ko/ko Mice

We analyzed short-term effects of rmIGF-1 treatment on the relative mRNA expression of osteopontin, osteocalcin and Col1a1 over time (0, 30, 120 and 240 min) in male and female tibias (Figure 3). Overall effects of genotype (F1.114 > 4.96, p < 0.028), sex (F1.114 > 63.20, p < 0.001) and time (F3.114 > 3.62, p < 0.016) on the three bone remodeling markers were found. Significant interactions between genotype, sex and time were observed in the mRNA levels of Col1a1 and osteopontin (F3.114 = 6.01, p = 0.001; F3.114 = 4.09, p = 0.009 respectively), suggesting that rmIGF-1 treatment affected bone remodeling markers, depending on sex and genotype over time.
In males, treatment with rmIGF-1 decreased the mRNA levels of Col1a1 and osteopontin in the tibia of both genotypes over time (30′, 120′ and 240′ versus 0′: */**/*** p < 0.05/0.01/0.001; Figure 3A,B). In contrast, rmIGF-1 treatment increased the mRNA levels of osteocalcin in the tibia of Pappa2ko/ko males, but not Pappa2wt/wt males, at 30′ and 240′ after administration (30′ and 240′ versus 0′: */** p < 0.05/0.01; Figure 3C). No sustained differences between genotypes (Pappa2ko/ko versus Pappa2wt/wt males) were found in the mRNA levels of the three bone remodeling markers.
In females, treatment with rmIGF-1 did not modify the mRNA levels of Col1a1 or osteopontin in the tibia of Pappa2wt/wt mice (Figure 3D,E). However, Col1a1 and osteopontin mRNA levels were significantly increased over time in Pappa2ko/ko female tibia (30′, 120′ and 240′ versus 0′: **/*** p < 0.01/0.001). Treatment with rmIGF-1 induced a transitory increase in osteocalcin mRNA levels of the Pappa2wt/wt female tibia (30′ and 120′ versus 0′: *p < 0.05), which gradually returned to normality over time (Figure 3F). However, no changes in osteocalcin mRNA levels in the tibia of Pappa2ko/ko females were found after treatment (Figure 3F). rmIGF-1 treatment-related differences between genotypes (Pappa2ko/ko versus Pappa2wt/wt females) were observed in the tibia mRNA levels of Col1a1 and osteopontin over time (30′: ##/### p < 0.01/0.001; 120′ and 240′: ### p < 0.001; Figure 3D,E). Basal differences between genotypes (Pappa2ko/ko versus Pappa2wt/wt females: ## p < 0.01) in the mRNA levels of osteocalcin were not observed after rmIGF-1 treatment (Figure 3F).

3. Discussion

In the present study, we showed that acute treatment with recombinant IGF-1 modulates bone composition and remodeling in Pappa2-deficient mice, in accordance with human clinical studies [25,26,27,28,29,30,31,32,33,34]. The effects of Pappa2 deficiency itself, as well as the short-term response to rmIGF-1 treatment on bone composition parameters, and the expression of relevant components of local IGF-1 system (Igfbp3, Igfbp5, Igfals) and bone remodeling (Col1a1, osteopontin, osteocalcin) were shown to be sex-dependent. Bone composition was analyzed using physical-chemical approaches to detect changes in bone crystallinity and relevant parameters (mineral-to-collagen matrix ratio and carbonate substitution) that reflect the relative content of bone compounds containing phosphates (v1v3PO43−), carbonates (v2CO32−) and amides I (vC=O), including mineral crystallinity (a degree of order in a solid) and collagen maturity (collagen crosslink ratio) in bone. The main results of the present study are as follows: (1) Pappa2 KO in mice of both sexes reduces body length, and bone length and weight, as expected from previous studies [30,31,32,33]; (2) Pappa2 KO in mice alters crystallographic parameters (hydroxyapatite crystallinity and crystallite size) in the femur of males, but not in that of females; (3) Pappa2 KO in mice impairs the M/M ratio and carbonate substitution, as well as mineral crystallinity in the femur of males, but not in females; (4) Pappa2 KO in mice of both sexes reduces collagen maturity; and (5) Pappa2 KO in female mice specifically increases the mRNA expression of Igfbp3, osteopontin (a marker of bone resorption) and osteocalcin (a marker of bone formation) in the tibia, but not in that of males. These results suggest that Pappa2 deficiency alters bone length, weight and composition, probably through changes in bone remodeling, in a sex-dependent manner.
A single administration of rmIGF-1 to adult mice induced short-term effects on bone composition and remodeling in a sex and genotype-dependent manner. The main results are as follows: (1) There is an increase in the M/M ratio and decreases in carbonate substitution and mineral crystallinity in Pappa2 KO mice of both sexes (Pappa2 WT mice showed a short transitory effect of rmIGF-1 on these parameters); (2) Administration of rmIGF-1 resulted in a specific increase in collagen maturity in Pappa2 KO females (no effect of rmIGF-1 in Pappa2 WT females); and (3) Specific increases in the mRNA expression of Igfbp3, Igfbp5, Col1a1 and osteopontin in Pappa2 KO females (no effects of rmIGF-1 in Pappa2 WT females) were also observed. Except in the higher expressions of Igfbp3 in Pappa2 KO males, no further differences between genotypes in the local IGF-1 system or bone remodeling markers were found in males.
These results suggest that rmIGF-1 treatment at a dose of 0.3 mg/kg may facilitate changes in bone composition, as addressed by parameters that reflect relative amounts of bone compounds containing phosphates, carbonates and amides I, which in turn, are controlled by local IGF-1 signaling and molecular mechanisms of bone mass homeostasis, such as a balance between bone resorption and formation. Some of these responses to exogenous IGF-1 were genotype-dependent. For instance, specific increases in Igfbp5 and/or Igfbp3 in the bone of Pappa2 KO mice likely reflect the requirement for PAPP-A2 in this response, as the exogenous IGF-1 can become locally bound in ternary complexes. Clinically, these results further contribute to our understanding of the therapeutic efficacy of recombinant IGF-1 in patients who display a novel syndrome characterized by short stature, skeletal abnormalities and increased formation of ternary complexes due to mutations in PAPP-A2 resulting in low IGF-1 bioactivity [25,27,28,29].
Many of the results observed here were sex-dependent and sex-specific regulation of bone properties and skeletal growth by GH/IGF-1 signaling system [5,7,35,36,37], including IGFALS, IGFBP-2, IGFBP-5, IGFBP-4, PAPP-A and PAPP-A2, have been reported [20,33,38,39,40,41]. Sex differences were also observed in basal bone physiology of Pappa2 deficient mice. Sex effects reflect an overall lower expression of Igfbp5 and Igfals, likely resulting from restricted IGF-1 production, could participate in the shorter bone length (12% less) and lower weight (34% less) compared to male bone. Sex effects are also associated with an overall difference in hydroxyapatite crystallinity, as well as M/M ratio, carbonate substitution and collagen maturity parameters, which were calculated from the relative content of bone compounds containing phosphates (v1v3PO43−), carbonates (v2CO32−) and amides I (vC=O). It is clear that sex steroid hormones are implicated in the differences between males and females in expression levels of components of the GH/IGF-1 axis and skeletal structure [7,42]. In humans, sex steroids contribute to the differences in pubertal growth that are mainly caused by greater periosteal expansion, smaller marrow diameter and greater longitudinal growth velocity and bone mass in males, compared to females [43,44,45], as well as to the earlier pubertal rise in GH and IGF-1 in girls than in boys and protection from age-related bone loss [6,42]. Consequently, bone strength shows a greater gain during postnatal growth and less decline during aging in males than in females [7,46]. In this context, we propose that sex-specific control of IGF-1 bioavailability through regulation of IGF ternary complexes could modify bone composition and at least partially explain the lower mechanical strength in the female bone [47]. Future studies should elucidate whether PAPP-A2 deficiency compromises the effects of estrogens and androgens in bone remodeling during growth and aging.
Constitutive and osteoblast-specific deletion of Pappa2 in mice has been described to affect postnatal skeletal growth, including bone mineral density, in a sex and age-dependent manner [31,32]. Interestingly, the bone of male Pappa2ko/ko mice has been described to be more similar to that of WT females than WT males [31]. Compared to WT males, our results indicated that the femur of Pappa2 ko/ko males was shorter (up to 22% less), weighed less (up to 34% less), had a smaller crystallite size and decreases in carbonate substitution (relative amount of carbonates), mineral crystallinity and collagen maturity, and exhibited increases in sample crystallinity and M/M ratio (relative amount of phosphates). However, these alterations in bone composition in the femur of Pappa2 ko/ko males were not accompanied by significant changes in the local IGF system or bone remodeling. In contrast, the significant reduction in bone length and weight (15–18% less) in Pappa2ko/ko female mice was associated with lower collagen maturity and a higher expression of Igfbp3. In this regard, bone turnover mechanisms could involve circulating factors such as IGFBP-3 or IGFBP-5 [33]. However, unlike Igfbp3, we found no genotype effect in the bone expression of Igfbp5 and Igfals, which is not consistent with previously described changes in its circulating levels [33,34]. These authors reported that Pappa2ko/ko mice of both sexes had higher serum levels of IGFBP-5 than their WT counterparts at 19 weeks, although the difference was not significant at 30 weeks [33]. Additionally, in a report where male and female mice were not studied separately [34], circulating levels of IGFBP-5 and IGFBP-3 were higher and lower, respectively, in the serum of Pappa2ko/ko mice at six weeks of age. These results are in partial contrast with those in male Pappa2ko/ko mice showing increased Igfbp3 expression in bone, as shown here, and those in patients with PAPP-A2 deficiency showing increased circulating levels of IGFBP-3 [28]. This apparent contradiction could reflect differing roles and regulation of IGFBPs in endocrine versus autocrine/paracrine signaling [20,48,49,50,51,52]. Indeed, no effects of Pappa2 deletion on the expression of these factors in the liver or kidney were described [34], suggesting tissue specific regulation of some members of the local IGF system. Moreover, locally generated IGF-1 can regulate bone growth in response to GH actions in liver-specific GH-receptor KO mice [53].
Molecular mechanisms balancing bone resorption by osteoclasts and bone formation by osteoblasts reflect the rate of bone turnover and remodeling [9]. In the present study, bone expression of osteopontin and osteocalcin (bone markers implicated in bone resorption and bone formation respectively) were increased in Pappa2ko/ko females. However, no change was found in Col1a1 expression, a major component of type I collagen that strengthens bone. These results suggest that higher bone turnover may also associate with lower collagen maturity in the bone of Pappa2ko/ko females. Although circulating levels of bone remodeling markers were not measured here, and these results contrast with the lower circulating levels of other markers of bone resorption (TRACP 5b) and bone formation (PINP), previously described in the plasma of female mice with Pappa2 deletion [33]. Again, this could reflect differences between local and circulating mechanisms controlling bone mass homeostasis. Bone turnover markers can also be modified by factors, such as feeding (lower resorption), bone loss and fractures (increased levels of all markers), and sources other than bone, such as platelets, liver, lungs and heart, can contribute significantly to circulating levels [54]. Higher levels of all bone turnover markers are highly correlated with the increased rate of bone loss in women [54,55,56], as observed here in Pappa2ko/ko females. Overall, our data suggest that an elevated rate of bone turnover, including lower collagen crosslink ratio, is a key determinant of bone immaturity in Pappa2 deficiency.
The changes in bone mineral composition, resulting from ionic substitution in biological hydroxyapatite, can induce alterations in hexagonal architecture, collagen structure and crystallinity affecting bone resistance or fragility [57,58,59,60,61,62,63,64,65]. Phosphate and carbonate substitutions in the apatite structure are responsible for changes in the degree of crystallinity, weakening the bonds and increasing mineral solubility [62,63]. Whereas, amide content in collagen-containing structures confers flexibility [64]. Carbonated hydroxyapatite contributes to a critical structure that affects mechanical strength of bone [65,66,67]. Here, the higher crystallinity and smaller crystallite size of the resulting hydroxyapatite are accompanied by a higher mineral-to-matrix ratio (relative amount of phosphates) and lower carbonate substitution and mineral crystallinity (related to crystallite size and perfection) in the femur of both WT females and Pappa2ko/ko males, compared to WT males. This is consistent with previous reports showing lower bone mineral density in Pappa2 transgenic mice in a sex-dependent manner [33]. Clinically, the fragility and fracture risk, related to untreated osteoporotic bone are associated with increases in cancellous crystallinity and mineral-to-matrix ratio [54,68]. Elevated levels of carbonate substitution were also found in fracture and osteoporosis cases [68]. Together, our data suggest that Pappa2 deletion results in the alteration of key parameters of bone composition that could denote a level of bone fragility.
In patients with PAPP-A2 deficiency recombinant human IGF-1 (rhIGF-1) was employed [28,29], but recombinant human PAPP-A2 could be a promising therapy [69]. Benefits of rhIGF-1 treatment include improved growth and increased bone mineral density and trabecular structure [28,29]. The administration of rhIGF-1 improves bone formation in aged mice [35], an effect that was markedly potentiated when combined with IGFBP-3 in ovariectomized rats [70], suggesting possible indications of long-term treatment, even after adult height is reached. In the present study, acute rmIGF-1 treatment induced a sex- and genotype-specific effect on bone composition, mostly reflected in short-term substitutions of the ionic content of phosphates, carbonates and amides I. In Pappa2ko/ko mice (males and females), rmIGF-1 increases the M/M ratio and decreases the carbonate substitution and mineral crystallinity over time, likely increasing bone strength and stiffness, while ductility decreased [61,62,68,71]. These rmIGF-1 induced changes in bone composition also include an increase in collagen maturity of the bone of Pappa2ko/ko females specifically, as this effect was not observed in Pappa2wt/wt females or Pappa2ko/ko males. Moreover, rmIGF-1-induced changes in the bone composition of Pappa2ko/ko females was accompanied by higher expression of Igfbp3 and Igfbp5, suggesting an acute response to increased IGF-1 bioavailability and signaling in a context of Pappa2 deficiency. Interestingly, higher expression of osteopontin and Col1a1 that was also specifically found in Pappa2ko/ko females after rmIGF-1 treatment, suggests an up-regulation of bone matrix resorption/formation. In this case, the concurrence of both higher collagen maturity and increased Col1a1 expression in the bone of rmIGF-1-treated Pappa2ko/ko females may trigger the correct collagenous matrix formation that can underlie bone strength [9]. The short-term modulation of bone mineral composition, the local IGF-1 system and bone remodeling in a sex and genotype-dependent manner may provide relevant insights into the therapeutic efficacy of recombinant IGF-1. Indeed, the increased expression of Igfbp3 and Igfbp5 in females with Pappa2 deletion, in response to rmIGF-1 treatment, may compromise long-term IGF-1 bioavailability. It could also be associated with an up-regulation of bone remodeling as assessed by increased expression of osteopotin and Col1a1.
In summary, our results support sex-specific regulation of bone composition and remodeling by PAPP-A2. Moreover, Pappa2 deletion altered the response to rmIGF-1 treatment, as indicated by parameters of bone mineral content, the local IGF-1 system and bone resorption/formation, in a sex specific manner. Collectively, our results support the therapeutic impact of IGF-1 to improve bone strength and density, but suggest that its clinical efficacy may differ depending on the sex of the patient with postnatal growth deficiency. However, the scarce experience treating patients with PAPP-A2 deficiency seems to indicate that both sexes respond adequately to this treatment [28].

4. Materials and Methods

All procedures were conducted in strict adherence to the principles of laboratory animal care (National Research Council, Neuroscience CoGftUoAi, Research B, 2003) following the European Community Council Directive (86/609/EEC) and were approved by the Ethical Committee of the University of Málaga (Ref. [69]—2016H). Special care was taken to minimize the suffering and number of animals necessary to perform the procedures.

4.1. Animals

Adult male and female mice (C57BL/6 background) with constitutive Pappa2 gene deletion (Pappa2ko/ko) and littermate controls (Pappa2wt/wt) were generated as previously described [31]. Mice were housed on a reverse 12-h light/dark cycle (lights off at 8:00 a.m.) in a humidity and temperature-controlled (22 ± 1 °C) vivarium. Standard rodent food and tap water were available ad libitum. Body weight and body length (including the tail) were monitored. Mice were genotyped by PCR using ear-clip tissue, as previously described [34].

4.2. Drugs

Recombinant murine IGF-1 (rmIGF-1; cat. no. 250-19; PeproTech, Inc., Rocky Hill, NJ, USA) was prepared by dissolving in 0.9% saline. The rmIGF-1 solution was injected subcutaneously once at a dose of 0.3 mg/kg in a volume of 2 mL/kg. Pappa2wt/wt and Pappa2ko/ko mice (males and females) were sacrificed 30, 120 and 240 min after rmIGF-1 administration. The route, dose and timing were selected based on previous studies of bone formation in aged mice [72].

4.3. Sample Collection

Adult mice (8 months of age) were sacrificed by decapitation after the administration of Equitesin® (3 mg/kg). Both femurs and tibias were extracted, cleaned of adjacent soft tissue, and immediately frozen in liquid nitrogen. Bones were weighed, and their length measured using a caliper. Femur epiphysis and metaphysis were removed and diaphysis were isolated. Frozen bone collections were stored at −80 °C until XRD, ATR-FTIR and RT-qPCR analyses.

4.4. Sample Preparation

Both femur diaphysis per mouse were pooled and pulverized in liquid nitrogen using a 6770 Freezer Mill (SPEX CertiPrepFreezerMill, Stanmore, London, UK). The cryogenic milling was carried out under mild conditions (cycles: 2; run time: 2 min; rate: 9 cps) to avoid altering the crystallinity of the materials or the spectral levels of the compounds under study. The resulting powder (50–100 µg particle size) was collected (~250 mg) and kept in a −80° freezer until XRD and ATR-FTIR analyses.

4.5. X-ray Powder Diffraction

Each femur diaphysis sample (~100 mg) was analyzed using an Empyrean Malvern Panalytical automated X-ray diffractometer (Malvern Panalytical, Malvern, United Kingdom) and Rietveld refinement [73,74,75,76]. The patterns of sample crystallinity and crystallite size were collected with a step size of 0.017° (2θ) and 300 sec/step using Cu-Kα (λ = 1.540598 Å) radiation from a tube operated at an accelerating voltage of 45 kV and a current of 35 mA. The (002) peak was baselined from 4° to 80° (2θ) for 30 min and fitted with a Lorentzian curve to determine the peak broadening based on its full width at half maximum (Figure S1A). Identification of amorphous phase and pure crystalline material was performed with reference to an external standard and the database supplied by the International Centre for Diffraction Data (Powder Diffraction File no. 84-1998), Inorganic Crystal Structure Database and Crystallography Open Database (COD no. 9010050; RRID:SCR_005874). Sample crystallinity (the degree of order in a solid) is defined as the quotient of enthalpy difference between pure amorphous phase and the sample enthalpy over the difference of pure amorphous and pure crystalline material (external standard). Percentage of crystallinity is calculated by: (total area of crystalline peaks) · 100/(total area of crystalline and amorphous peaks). The Scherrer equation (Dv = K · λ/β002 · cosθ) and Williamson-Hall method were used to calculate crystallite size (LVol-IB, nm); where Dv is the volume weighted crystallite size, K is the Scherrer constant with a value of 1, λ is the x-ray wavelength used, and β002 is the integral breadth of the (002) reflection or length of the apatite crystals along the c-axis. The R-Bragg factor, cell volume, crystal linear absorbance coefficient (1/cm) and crystal density (g/cm3) were also checked. Three patterns were performed and a mean pattern was obtained for each sample.

4.6. ATR-FTIR Spectroscopy

The infra-red (IR) analysis of each femur diaphysis sample (~100 mg) was carried out in a Bruker Vertex 70 Fourier Transform (FT)-IR spectrophotometer (Bruker Corporation, Billerica, MA, USA). We worked with attenuated total reflectance (ATR) using a Golden Gate System of Individual Reflection [77,78,79]. The material of our internal reflection element was ZnSe (20,000–500 cm−1). For the acquisition of spectra, a standard spectral resolution of 4 cm−1 in the spectral range of 500–4000 cm−1 was used, as well as 64 accumulations per sample. The background spectrum in all cases was the air. For the analysis of the raw spectra, the v1v3PO43− bands were baselined from 1200 to 900 cm−1, the v2CO32− band from 890 to 850 cm−1, and the amide I band from 1730 to 1585 cm−1. Spectral analysis was performed in triplicate and a mean spectrum was obtained for each sample (Figure S1B). After curve-fitting of every individual (not smoothing) spectrum, position, height and area under the curves (baseline correction) were measured.
The following parameters that reflect the bone tissue compositional properties were calculated [80,81,82]: (1) Mineral-to-organic matrix (M/M) ratio, an index of tissue mineral content that characterizes the relative amount of phosphate per amount of collagen present, and is calculated by the ratio of the integrated areas of the respective raw peaks of v1v3PO43− (900–1200 cm−1) and amide I (1585–1730 cm−1); (2) carbonate substitution (C/P ratio), an index of phosphate-to-carbonate-substituted apatites that characterizes the extent to which carbonate substitutes into mineral lattice, and is calculated by the ratio of the integrated areas of the respective raw peaks of v2CO32− (850–890 cm−1) and v1v3PO43− (900–1200 cm−1); (3) mineral crystallinity or maturity (1030/1020 cm−1 intensity ratio), a degree of order in a solid that is related to crystal size and perfection; and (4) collagen maturity (1660/1690 cm−1 intensity ratio), an index related to the ratio of mature, non-reducible collagen crosslinks to immature, reducible collagen crosslinks. We applied the second derivatives of the raw data from ATR-FTIR spectra to determine specific peaks at ~1030, ~1020, ~1660 and ~1690 cm−1, and improve the accuracy of quantification of mineral maturity and collagen crosslink ratio (Figure S1C).

4.7. RNA Isolation and RT-qPCR Analysis

Frozen tibias were pulverized using a Qiagen TissueLyser II sample disruptor (Qiagen, Hilden, Germany). We performed real-time PCR, as described previously [83], using specific sets of primer probes from TaqMan® Gene Expression Assays (Pappa2: Mm01284029_m1, amplicon length: 70; Igfbp3: Mm01187817_m1, amplicon length: 78; Igfbp4: Mm00494922_m1, amplicon length: 76; Igfbp5: Mm00516037_m1, amplicon length: 70; Igfals: Mm01962637_s1, amplicon length: 106; Stc2: Mm00441560_m1, amplicon length: 60; Col1a1: Mm00801666_g1, amplicon length: 89; osteopontin (Opn, Spp1): Mm00436767_m1, amplicon length: 114; osteocalcin (Bglap): Mm04313826_mH, amplicon length: 110; ThermoFisher Scientific, Waltham, MA, USA). The total RNA quantity was extracted from tibias using the Trizol® method according to the manufacturer’s instructions (ThermoFisher Scientific, Waltham, MA, USA). Isolated RNA samples were quantified using a spectrophotometer to ensure A260/280 ratios of 1.8–2.0. After the reverse transcript reaction from 1 μg of mRNA, a quantitative real-time reverse transcription polymerase chain reaction (qPCR) was performed in a CFX96TM Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA) using FAM (fluorescein amidites) dye labeled format for the TaqMan® Gene Expression Assays (ThermoFisher Scientific, Waltham, MA, USA). A melting curve analysis was performed to ensure that only a simple product per replicate was amplified. After analyzing several reference genes, values obtained from the tibias were normalized in relation to Actb levels (Mm02619580_g1, amplicon length: 143; ThermoFisher Scientific, Waltham, MA, USA), which was found not to vary significantly between experimental groups.

4.8. Data Analysis

Data are presented as means ± S.E.M. and the “n” in figure legends indicates the number of animals per group. Data were normally distributed. For statistical analysis, we used GraphPad Prism 6.0 (GraphPad Software, San Diego, CA, USA) and IBM SPSS software 23.0 (SPSS Inc., Chicago, IL, USA) in order to apply two and three-way ANOVA (genotype, sex and time as factors) followed by Tukey-corrected tests or simple effect analyses where appropriate. A p < 0.05 indicates statistical significance.

Supplementary Materials

The following are available online at https://www.mdpi.com/article/10.3390/ijms22084048/s1, Figure S1: (A) Representative diffractogram of bone hydroxyapatite-(CaOH) in the femur of Pappa2wt/wt and Pappa2ko/ko mice (males and females) by X-ray powder diffraction (XRD). (B) Representative ATR-FTIR spectra of the raw data. The typical ATR-FTIR spectral bands (peaks) that define amide, carbonate and phosphate groups at specific wavenumbers were obtained. (C) Second derivatives of the ATR-FTIR spectra define the amide I band positions in the 1585–1730 cm−1 region (left) and the v1v3PO43− band positions in the 900–1200 cm−1 region (right). The peaks at 1660 and 1690 cm−1 (red lines in the left) correspond to the pyridinoline and dehydrohydroxylysinonorleucine collagen crosslinks, respectively. The respective peaks at ~960, ~1020, 1030 and 1115 cm−1 (red lines in the right) correspond to v1PO43−, PO43− in stoichiometric apatites, HPO42− and/or CO32− in nonstoichiometric poorly crystalline apatites and other poorly crystalline apatites, respectively; Table S1: Quantitative analysis of crystallographic properties of bone hydroxyapatite-(CaOH) in the femur of Pappa2wt/wt and Pappa2ko/ko mice (males and females) by XRD; Table S2: Quantitative analysis of spectral levels (actual absorbance) of specific ionic contents in the femur of Pappa2wt/wt and Pappa2ko/ko mice (males and females).

Author Contributions

Conceptualization, J.A. and J.S.; methodology, L.R. and A.V.; formal analysis, L.R., A.V., A.J.L.-G. and R.T.; data curation, L.R., P.R., J.A.C., J.A. and J.S.; writing—original draft preparation, L.R., J.A. and J.S.; writing—review and editing, J.K.C., S.M.-d.-l.-H., F.R.d.F. and J.A.C.; funding acquisition, J.K.C., J.A.C., J.A. and J.S. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by Instituto de Salud Carlos III (ISCIII), Ministerio de Economía y Competitividad (MINECO) and European Regional Development Funds-European Union (ERDF-EU), grant number PI16/01374 and PI19/00343 (J.S.), PI16/00485 and PI19/00166 (J.A.), and BFU2017–82565-C2-1-R (J.A.C.); NSERC (Canada) Discovery Grant, grant number, 2016-04047 (J.K.C.). J.S. (CPII17/00024) and P.R. (CP19/00068) hold “Miguel Servet” research contracts and A.J.L.-G. holds a i-PFIS research contract (IFI18/00042), all of them from the National System of Health, ISCIII, ERDF-EU.

Institutional Review Board Statement

The study was conducted according to the principles of laboratory animal care (National Research Council, Neuroscience CoGftUoAi, Research B, 2003) fol-lowing the European Community Council Directive (86/609/EEC) and approved by the Ethics Committee for Animal Research of the University of Málaga, Spain (Code: 46-2019-A; date: 15 July 2019).

Informed Consent Statement

Not applicable.

Data Availability Statement

The data that support the findings of this study are available on reasonable request from the corresponding author.

Acknowledgments

The authors thank to Cristina Capel, Laura León and Lourdes Salcedo for excellent technical assistance in XRD and ATR-FTIR spectroscopy supported by the SCAI Facility of the University of Malaga.

Conflicts of Interest

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

References

  1. Mohan, S.; Richman, C.; Guo, R.; Amaar, Y.; Donahue, L.R.; Wergedal, J.; Baylink, D.J. Insulin-like growth factor regulates peak bone mineral density in mice by both growth hormone-dependent and -independent mechanisms. Endocrinology 2003, 144, 929–936. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  2. Govoni, K.E.; Baylink, D.J.; Mohan, S. The multi-functional role of insulin-like growth factor binding proteins in bone. Pediatr. Nephrol. 2005, 20, 261–268. [Google Scholar] [CrossRef] [PubMed]
  3. Baylink, D.; Lau, K.H.; Mohan, S. The role of IGF system in the rise and fall in bone density with age. J. Musculoskelet Neuronal Interact. 2007, 7, 304–305. [Google Scholar] [PubMed]
  4. Tritos, N.A.; Klibanski, A. Effects of Growth Hormone on Bone. Prog. Mol. Biol. Transl. Sci. 2016, 138, 193–211. [Google Scholar] [CrossRef]
  5. Yakar, S.; Isaksson, O. Regulation of skeletal growth and mineral acquisition by the GH/IGF-1 axis: Lessons from mouse models. Growth Horm IGF Res. 2016, 28, 26–42. [Google Scholar] [CrossRef] [Green Version]
  6. Rogol, A.D. Sex steroids, growth hormone, leptin and the pubertal growth spurt. Endocr. Dev. 2010, 17, 77–85. [Google Scholar] [CrossRef]
  7. Liu, Z.; Mohan, S.; Yakar, S. Does the GH/IGF-1 axis contribute to skeletal sexual dimorphism? Evidence from mouse studies. Growth Horm IGF Res. 2016, 27, 7–17. [Google Scholar] [CrossRef] [Green Version]
  8. Southmayd, E.A.; De Souza, M.J. A summary of the influence of exogenous estrogen administration across the lifespan on the GH/IGF-1 axis and implications for bone health. Growth Horm IGF Res. 2017, 32, 2–13. [Google Scholar] [CrossRef]
  9. Raggatt, L.J.; Partridge, N.C. Cellular and molecular mechanisms of bone remodeling. J. Biol. Chem. 2010, 285, 25103–25108. [Google Scholar] [CrossRef] [Green Version]
  10. Baker, J.; Liu, J.P.; Robertson, E.J.; Efstratiadis, A. Role of insulin-like growth factors in embryonic and postnatal growth. Cell 1993, 75, 73–82. [Google Scholar] [CrossRef]
  11. Powell-Braxton, L.; Hollingshead, P.; Warburton, C.; Dowd, M.; Pitts-Meek, S.; Dalton, D.; Gillett, N.; Stewart, T.A. IGF-I is required for normal embryonic growth in mice. Genes Dev. 1993, 7, 2609–2617. [Google Scholar] [CrossRef]
  12. He, J.; Rosen, C.J.; Adams, D.J.; Kream, B.E. Postnatal growth and bone mass in mice with IGF-I haploinsufficiency. Bone 2006, 38, 826–835. [Google Scholar] [CrossRef]
  13. Wang, Y.; Nishida, S.; Sakata, T.; Elalieh, H.Z.; Chang, W.; Halloran, B.P.; Doty, S.B.; Bikle, D.D. Insulin-like growth factor-I is essential for embryonic bone development. Endocrinology 2006, 147, 4753–4761. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  14. Woods, K.A.; Camacho-Hübner, C.; Savage, M.O.; Clark, A.J. Intrauterine growth retardation and postnatal growth failure associated with deletion of the insulin-like growth factor I gene. N. Engl. J. Med. 1996, 335, 1363–1367. [Google Scholar] [CrossRef] [PubMed]
  15. Abuzzahab, M.J.; Schneider, A.; Goddard, A.; Grigorescu, F.; Lautier, C.; Keller, E.; Kiess, W.; Klammt, J.; Kratzsch, J.; Osgood, D.; et al. Intrauterine Growth Retardation (IUGR) Study Group. IGF-I receptor mutations resulting in intrauterine and postnatal growth retardation. N. Engl. J. Med. 2003, 349, 2211–2222. [Google Scholar] [CrossRef] [PubMed]
  16. Miyakoshi, N.; Richman, C.; Kasukawa, Y.; Linkhart, T.A.; Baylink, D.J.; Mohan, S. Evidence that IGF-binding protein-5 functions as a growth factor. J. Clin. Investig. 2001, 107, 73–81. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  17. Overgaard, M.T.; Boldt, H.B.; Laursen, L.S.; Sottrup-Jensen, L.; Conover, C.A.; Oxvig, C. Pregnancy-associated plasma protein-A2 (PAPP-A2), a novel insulin-like growth factor-binding protein-5 proteinase. J. Biol. Chem. 2001, 276, 21849–21853. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  18. Mohan, S.; Baylink, D.J. IGF-binding proteins are multifunctional and act via IGF-dependent and -independent mechanisms. J. Endocrinol. 2002, 175, 19–31. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  19. Bunn, R.C.; Fowlkes, J.L. Insulin-like growth factor binding protein proteolysis. Trends Endocrinol. Metab. 2003, 14, 176–181. [Google Scholar] [CrossRef]
  20. Salih, D.A.; Mohan, S.; Kasukawa, Y.; Tripathi, G.; Lovett, F.A.; Anderson, N.F.; Carter, E.J.; Wergedal, J.E.; Baylink, D.J.; Pell, J.M. Insulin-like growth factor-binding protein-5 induces a gender-related decrease in bone mineral density in transgenic mice. Endocrinology 2005, 146, 931–940. [Google Scholar] [CrossRef] [Green Version]
  21. Ranke, M.B. Insulin-like growth factor binding-protein-3 (IGFBP-3). Best Pract. Res. Clin. Endocrinol. Metab. 2015, 29, 701–711. [Google Scholar] [CrossRef] [PubMed]
  22. Yakar, S.; Rosen, C.J.; Beamer, W.G.; Ackert-Bicknell, C.L.; Wu, Y.; Liu, J.L.; Ooi, G.T.; Setser, J.; Frystyk, J.; Boisclair, Y.R.; et al. Circulating levels of IGF-1 directly regulate bone growth and density. J. Clin. Investig. 2002, 110, 771–781. [Google Scholar] [CrossRef] [PubMed]
  23. Yakar, S.; Wu, Y.; Setser, J.; Rosen, C.J. The role of circulating IGF-I: Lessons from human and animal models. Endocrine 2002, 19, 239–248. [Google Scholar] [CrossRef]
  24. Dauber, A.; Muñoz-Calvo, M.T.; Barrios, V.; Domené, H.M.; Kloverpris, S.; Serra-Juhé, C.; Desikan, V.; Pozo, J.; Muzumdar, R.; Martos-Moreno, G.Á.; et al. Mutations in pregnancy-associated plasma protein A2 cause short stature due to low IGF-I availability. EMBO Mol. Med. 2016, 8, 363–374. [Google Scholar] [CrossRef] [PubMed]
  25. Argente, J.; Pérez-Jurado, L.A. Genetic causes of proportionate short stature. Best Pract. Res. Clin. Endocrinol. Metab. 2018, 32, 499–522. [Google Scholar] [CrossRef]
  26. Argente, J.; Pérez-Jurado, L.A. History and clinical implications of PAPP-A2 in human growth: When reflecting on idiopathic short stature leads to a specific and new diagnosis: Understanding the concept of “low IGF-I availability”. Growth Horm. IGF Res. 2018, 40, 19. [Google Scholar] [CrossRef] [PubMed]
  27. Ranke, M.B.; Wit, J.M. Growth hormone—past, present and future. Nat. Rev. Endocrinol. 2018, 14, 285–300. [Google Scholar] [CrossRef]
  28. Muñoz-Calvo, M.T.; Barrios, V.; Pozo, J.; Chowen, J.A.; Martos-Moreno, G.Á.; Hawkins, F.; Dauber, A.; Domené, H.M.; Yakar, S.; Rosenfeld, R.G.; et al. Treatment with recombinant human insulin-like growth factor-1 improves growth in patients with PAPP-A2 deficiency. J. Clin. Endocrinol. Metab. 2016, 101, 3879–3883. [Google Scholar] [CrossRef] [Green Version]
  29. Hawkins-Carranza, F.G.; Muñoz-Calvo, M.T.; Martos-Moreno, G.Á.; Allo-Miguel, G.; Del Río, L.; Pozo, J.; Chowen, J.A.; Pérez-Jurado, L.A.; Argente, J. rhIGF-1 treatment increases bone mineral density and trabecular bone structure in children with PAPP-A2 deficiency. Horm Res. Paediatr. 2018, 89, 200–204. [Google Scholar] [CrossRef]
  30. Conover, C.A.; Boldt, H.B.; Bale, L.K.; Clifton, K.B.; Grell, J.A.; Mader, J.R.; Mason, E.J.; Powell, D.R. Pregnancy-associated plasma protein-A2 (PAPP-A2): Tissue expression and biological consequences of gene knockout in mice. Endocrinology 2011, 152, 2837–2844. [Google Scholar] [CrossRef] [Green Version]
  31. Christians, J.K.; de Zwaan, D.R.; Fung, S.H. Pregnancy associated plasma protein A2 (PAPP-A2) affects bone size and shape and contributes to natural variation in postnatal growth in mice. PLoS ONE 2013, 8, e56260. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  32. Amiri, N.; Christians, J.K. PAPP-A2 expression by osteoblasts is required for normal postnatal growth in mice. Growth Horm IGF Res. 2015, 25, 274–280. [Google Scholar] [CrossRef] [PubMed]
  33. Christians, J.K.; Amiri, N.; Schipilow, J.D.; Zhang, S.W.; May-Rashke, K.I. Pappa2 deletion has sex- and age-specific effects on bone in mice. Growth Horm. IGF Res. 2019, 44, 6–10. [Google Scholar] [CrossRef] [PubMed]
  34. Christians, J.K.; Bath, A.K.; Amiri, N. Pappa2 deletion alters IGFBPs but has little effect on glucose disposal or adiposity. Growth Horm. IGF Res. 2015, 25, 232–239. [Google Scholar] [CrossRef] [PubMed]
  35. Sandstedt, J.; Törnell, J.; Norjavaara, E.; Isaksson, O.G.; Ohlsson, C. Elevated levels of growth hormone increase bone mineral content in normal young mice, but not in ovariectomized mice. Endocrinology 1996, 137, 3368–3374. [Google Scholar] [CrossRef] [PubMed]
  36. Eckstein, F.; Lochmüller, E.M.; Koller, B.; Wehr, U.; Weusten, A.; Rambeck, W.; Hoeflich, A.; Wolf, E. Body composition, bone mass and microstructural analysis in GH-transgenic mice reveals that skeletal changes are specific to bone compartment and gender. Growth Horm. IGF Res. 2002, 12, 116–125. [Google Scholar] [CrossRef]
  37. Wu, Y.; Sun, H.; Basta-Pljakic, J.; Cardoso, L.; Kennedy, O.D.; Jasper, H.; Domené, H.; Karabatas, L.; Guida, C.; Schaffler, M.B.; et al. Serum IGF-1 is insufficient to restore skeletal size in the total absence of the growth hormone receptor. J. Bone Miner. Res. 2013, 28, 1575–1586. [Google Scholar] [CrossRef]
  38. Courtland, H.W.; DeMambro, V.; Maynard, J.; Sun, H.; Elis, S.; Rosen, C.; Yakar, S. Sex-specific regulation of body size and bone slenderness by the acid labile subunit. J. Bone Miner. Res. 2010, 25, 2059–2068. [Google Scholar] [CrossRef]
  39. DeMambro, V.E.; Clemmons, D.R.; Horton, L.G.; Bouxsein, M.L.; Wood, T.L.; Beamer, W.G.; Canalis, E.; Rosen, C.J. Gender-specific changes in bone turnover and skeletal architecture in igfbp-2-null mice. Endocrinology 2008, 149, 2051–2061. [Google Scholar] [CrossRef]
  40. Maridas, D.E.; DeMambro, V.E.; Le, P.T.; Nagano, K.; Baron, R.; Mohan, S.; Rosen, C.J. IGFBP-4 regulates adult skeletal growth in a sex-specific manner. J. Endocrinol. 2017, 233, 131–144. [Google Scholar] [CrossRef]
  41. Tanner, S.J.; Hefferan, T.E.; Rosen, C.J.; Conover, C.A. Impact of pregnancy-associated plasma protein-a deletion on the adult murine skeleton. J. Bone Miner. Res. 2008, 23, 655–662. [Google Scholar] [CrossRef] [Green Version]
  42. Olson, L.E.; Ohlsson, C.; Mohan, S. The role of GH/IGF-I-mediated mechanisms in sex differences in cortical bone size in mice. Calcif. Tissue Int. 2011, 88, 1–8. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  43. Seeman, E. During aging, men lose less bone than women because they gain more periosteal bone, not because they resorb less endosteal bone. Calcif. Tissue Int. 2001, 69, 205–208. [Google Scholar] [CrossRef] [PubMed]
  44. Riggs, B.L.; Khosla, S.; Melton, L.J., 3rd. Sex steroids and the construction and conservation of the adult skeleton. Endocr. Rev. 2002, 23, 279–302. [Google Scholar] [CrossRef] [PubMed]
  45. Christoforidis, A.; Maniadaki, I.; Stanhope, R. Growth hormone/insulin-like growth factor-1 axis during puberty. Pediatr. Endocrinol. Rev. 2005, 3, 5–10. [Google Scholar] [PubMed]
  46. Veldhuis, J.D.; Roemmich, J.N.; Richmond, E.J.; Rogol, A.D.; Lovejoy, J.C.; Sheffield-Moore, M.; Mauras, N.; Bowers, C.Y. Endocrine control of body composition in infancy, childhood, and puberty. Endocr. Rev. 2005, 26, 114–146. [Google Scholar] [CrossRef] [PubMed]
  47. Seeman, E. Estrogen, androgen, and the pathogenesis of bone fragility in women and men. Curr. Osteoporos Rep. 2004, 2, 90–96. [Google Scholar] [CrossRef]
  48. Bauss, F.; Lang, K.; Dony, C.; Kling, L. The complex of recombinant human insulin-like growth factor-I (rhIGF-I) and its binding protein-5 (IGFBP-5) induces local bone formation in murine calvariae and in rat cortical bone after local or systemic administration. Growth Horm. IGF Res. 2001, 11, 1–9. [Google Scholar] [CrossRef]
  49. Ning, Y.; Schuller, A.G.; Bradshaw, S.; Rotwein, P.; Ludwig, T.; Frystyk, J.; Pintar, J.E. Diminished growth and enhanced glucose metabolism in triple knockout mice containing mutations of insulin-like growth factor binding protein-3, -4, and -5. Mol. Endocrinol. 2006, 20, 2173–2186. [Google Scholar] [CrossRef]
  50. Mukherjee, A.; Rotwein, P. Insulin-like growth factor binding protein-5 in osteogenesis: Facilitator or inhibitor? Growth Horm. IGF Res. 2007, 17, 179–185. [Google Scholar] [CrossRef] [Green Version]
  51. Mukherjee, A.; Rotwein, P. Insulin-like growth factor-binding protein-5 inhibits osteoblast differentiation and skeletal growth by blocking insulin-like growth factor actions. Mol. Endocrinol. 2008, 22, 1238–1250. [Google Scholar] [CrossRef] [Green Version]
  52. Allard, J.B.; Duan, C. IGF-Binding Proteins: Why Do They Exist and Why Are There So Many? Front. Endocrinol. 2018, 9, 117. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  53. Fan, Y.; Menon, R.K.; Cohen, P.; Hwang, D.; Clemens, T.; DiGirolamo, D.J.; Kopchick, J.J.; Le Roith, D.; Trucco, M.; Sperling, M.A. Liver-specific deletion of the growth hormone receptor reveals essential role of growth hormone signaling in hepatic lipid metabolism. J. Biol. Chem. 2009, 284, 19937–19944. [Google Scholar] [CrossRef] [Green Version]
  54. Szulc, P. Bone turnover: Biology and assessment tools. Best Pract. Res. Clin. Endocrinol. Metab. 2018, 32, 725–738. [Google Scholar] [CrossRef] [PubMed]
  55. Ivaska, K.K.; Lenora, J.; Gerdhem, P.; Akesson, K.; Väänänen, H.K.; Obrant, K.J. Serial assessment of serum bone metabolism markers identifies women with the highest rate of bone loss and osteoporosis risk. J. Clin. Endocrinol. Metab. 2008, 93, 2622–2632. [Google Scholar] [CrossRef] [PubMed]
  56. Szulc, P.; Bauer, D.C. Biochemical markers of bone turnover in osteoporosis. In Osteoporosis, 4th ed.; Marcus, R., Feldman, D., Dempster, D.W., Luckey, M., Cauley, J.A., Eds.; Academic Press Elsevier: Amsterdam, The Netherlands, 2013; pp. 1573–1610. [Google Scholar]
  57. Boskey, A.L.; Imbert, L. Bone quality changes associated with aging and disease: A review. Ann. N. Y. Acad. Sci. 2017, 1410, 93–106. [Google Scholar] [CrossRef]
  58. Elliott, J.C. Calcium Phosphate Biominerals. In Phosphates: Geochemical, Geobiological and Material Importance, Reviews in Mineralogy and Geochemistry; Kohn, M.J., Rakovan, J., Hughes., J.M., Eds.; Mineralogical Society of America: Washington, DC, USA, 2002; Volume 48, pp. 427–454. [Google Scholar]
  59. Hughes, J.M.; Rakovan, J. The Crystal Structure of Apatite, Ca5(PO4)3(F,OH,Cl). In Phosphates: Geochemical, Geobiological and Material Importance, Reviews in Mineralogy and Geochemistry; Kohn, M.J., Rakovan, J., Hughes, J.M., Eds.; Mineralogical Society of America: Washington, DC, USA, 2002; Volume 48, pp. 1–12. [Google Scholar]
  60. Astala, R.; Stott, M.J. First principles investigation of mineral component of bone: CO3 substitutions in hydroxyapatite. Chem. Mater. 2005, 17, 4125–4133. [Google Scholar] [CrossRef]
  61. Pasteris, J.D.; Wopenka, B.; Freeman, J.J.; Rogers, K.; Valsami-Jones, E.; van der Houwen, J.A.; Silva, M.J. Lack of OH in nanocrystalline apatite as a function of degree of atomic order: Implications for bone and biomaterials. Biomaterials 2004, 25, 229–238. [Google Scholar] [CrossRef]
  62. Nakamura, M.; Hiratai, R.; Hentunen, T.; Salonen, J.; Yamashita, K. Hydroxyapatite with high carbonate substitutions promotes osteoclast resorption through osteocytelike cells. ACS Biomater. Sci. Eng. 2016, 2, 259–267. [Google Scholar] [CrossRef]
  63. Vallet-Regi, M.; Navarette, D.A. Nanoceramics in Clinical Use: From Materials to Applications, 2nd ed.; Royal Society of Chemistry: Cambridge, MA, USA, 2015. [Google Scholar]
  64. de Campos Vidal, B.; Mello, M.L.S. Collagen type I amide I band infrared spectroscopy. Micron 2011, 42, 283–289. [Google Scholar] [CrossRef]
  65. Awonusi, A.; Morris, M.D.; Tecklenburg, M.M. Carbonate assignment and calibration in the Raman spectrum of apatite. Calcif. Tissue Int. 2007, 81, 46–52. [Google Scholar] [CrossRef] [PubMed]
  66. Kamitakahara, M.; Ohtsuki, C.; Miyazaki, T. Coating of bone-like apatite for development of bioactive materials for bone reconstruction. Biomed. Mater. 2007, 2, R17. [Google Scholar] [CrossRef] [PubMed]
  67. Amenta, E.; King, H.E.; Petermann, H.; Uskoković, V.; Tommasini, S.M.; Macica, C.M. Vibrational spectroscopic analysis of hydroxyapatite in HYP mice and individuals with X-linked hypophosphatemia. Ther. Adv. Chronic. Dis. 2018, 9, 268–281. [Google Scholar] [CrossRef] [Green Version]
  68. Gourion-Arsiquaud, S.; Faibish, D.; Myers, E.; Spevak, L.; Compston, J.; Hodsman, A.; Shane, E.; Recker, R.R.; Boskey, E.R.; Boskey, A.L. Use of FTIR spectroscopic imaging to identify parameters associated with fragility fracture. J. Bone Miner. Res. 2009, 24, 1565–1571. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  69. Andrew, M.; Liao, L.; Fujimoto, M.; Khoury, J.; Hwa, V.; Dauber, A. PAPPA2 as a Therapeutic Modulator of IGF-I Bioavailability: In Vivo and in Vitro Evidence. J. Endocr. Soc. 2018, 2, 646–656. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  70. Narusawa, K.; Nakamura, T.; Suzuki, K.; Matsuoka, Y.; Lee, L.J.; Tanaka, H.; Seino, Y. The effects of recombinant human insulin-like growth factor (rhIGF)-1 and rhIGF-1/IGF binding protein-3 administration on rat osteopenia induced by ovariectomy with concomitant bilateral sciatic neurectomy. J. Bone Miner. Res. 1995, 10, 1853–1864. [Google Scholar] [CrossRef]
  71. Seeman, E. Overview of bone microstructure, and treatment of bone fragility in chronic kidney disease. Nephrology 2017, 22 (Suppl. S2), 34–35. [Google Scholar] [CrossRef] [Green Version]
  72. Fowlkes, J.L.; Thrailkill, K.M.; Liu, L.; Wahl, E.C.; Bunn, R.C.; Cockrell, G.E.; Perrien, D.S.; Aronson, J.; Lumpkin, C.K., Jr. Effects of systemic and local administration of recombinant human IGF-I (rhIGF-I) on de novo bone formation in an aged mouse model. J. Bone Miner. Res. 2006, 21, 1359–1366. [Google Scholar] [CrossRef] [Green Version]
  73. Figueiredo, M.; Fernando, A.; Martins, G.; Freitas, J.; Judas, F.; Figueiredo, H. Effect of the calcination temperature on the composition and microstructure of hydroxyapatite derived from human and animal bone. Ceram. Int. 2010, 36, 2383–2393. [Google Scholar] [CrossRef]
  74. Grynpas, M. The crystallinity of bone mineral. J. Miner. Sci. 1976, 11, 1691–1696. [Google Scholar] [CrossRef]
  75. Farlay, F.D.; Panczer, G.; Rey, C.; Delmas, P.; Boivin, G. Mineral maturity and crystallinity index are distinct characteristics of bone mineral. J. Bone Miner. Metab. 2010, 28, 433–445. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  76. Sa, Y.; Guo, Y.; Feng, X.; Wang, M.; Li, P.; Gao, Y.; Yang, X.; Jiang, T. Are different crystallinity-index-calculating methods of hydroxyapatite efficient and consistent? New J. Chem. 2017, 41, 5723–5731. [Google Scholar] [CrossRef]
  77. Surovell, T.A.; Stiner, M.C. Standardizing infra-red measures of bone mineral crystallinity: An experimental approach. J. Arch. Sci. 2000, 28, 633–642. [Google Scholar] [CrossRef] [Green Version]
  78. Dal Sasso, G.; Asscher, Y.; Angelini, I.; Nodari, L.; Artioli, G. A universal curve of apatite crystallinity for the assessment of bone integrity and preservation. Sci. Rep. 2018, 8, 12025. [Google Scholar] [CrossRef] [PubMed]
  79. Querido, W.; Ailavajhala, R.; Padalkar, M.; Pleshko, N. Validated Approaches for Quantification of Bone Mineral Crystallinity Using Transmission Fourier Transform Infrared (FT-IR), Attenuated Total Reflection (ATR) FT-IR, and Raman Spectroscopy. Appl. Spectrosc. 2018, 72, 1581–1593. [Google Scholar] [CrossRef] [PubMed]
  80. Paschalis, E.P.; Mendelsohn, R.; Boskey, A.L. Infrared assessment of bone quality: A review. Clin. Orthop. Relat. Res. 2011, 469, 2170–2178. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  81. Paschalis, E.P. Fourier Transform Infrared Imaging of Bone. Methods Mol. Biol. 2019, 1914, 641–649. [Google Scholar]
  82. Taylor, E.A.; Lloyd, A.A.; Salazar-Lara, C.; Donnelly, E. Raman and Fourier Transform Infrared (FT-IR) Mineral to Matrix Ratios Correlate with Physical Chemical Properties of Model Compounds and Native Bone Tissue. Appl. Spectrosc. 2017, 71, 2404–2410. [Google Scholar] [CrossRef]
  83. Rivera, P.; Ramírez-López, M.T.; Vargas, A.; Decara, J.; Vázquez, M.; Arco, R.; Gómez de Heras, R.; Argente, J.; Rodríguez de Fonseca, F.; Chowen, J.A.; et al. Perinatal free-choice of a high-calorie low-protein diet affects leptin signaling through IRS1 and AMPK dephosphorylation in the hypothalami of female rat offspring in adulthood. Acta Physiol. 2019, 226, e13244. [Google Scholar] [CrossRef] [PubMed]
Ijms 22 04048 g001 550
Figure 1. Quantitative analysis of the mineral-to-organic matrix (M/M) ratio, carbonate substitution (C/P ratio), mineral crystallinity and collagen maturity in the femur of Pappa2wt/wt and Pappa2ko/ko mice at 0′, 30′, 120′ and 240′ after rmIGF-1 administration in males (A–D) and females (E–H). Data are represented as mean ± S.E.M. (n = 6–9/group). Tukey-corrected tests: #/##/### p < 0.05/0.01/0.001 between genotypes (same time); */**/*** p < 0.05/0.01/0.001 versus 0′ (same genotype).
Figure 1. Quantitative analysis of the mineral-to-organic matrix (M/M) ratio, carbonate substitution (C/P ratio), mineral crystallinity and collagen maturity in the femur of Pappa2wt/wt and Pappa2ko/ko mice at 0′, 30′, 120′ and 240′ after rmIGF-1 administration in males (A–D) and females (E–H). Data are represented as mean ± S.E.M. (n = 6–9/group). Tukey-corrected tests: #/##/### p < 0.05/0.01/0.001 between genotypes (same time); */**/*** p < 0.05/0.01/0.001 versus 0′ (same genotype).
Ijms 22 04048 g001
Ijms 22 04048 g002 550
Figure 2. Relative mRNA levels of Igfbp3, Igfbp5 and Igfals in the tibia of Pappa2wt/wt and Pappa2ko/ko mice at 0′, 30′, 120′ and 240′ after rmIGF-1 administration in males (A–C) and females (D–F). Data are represented as mean ± S.E.M (n = 6–9/group). Tukey-corrected tests: #/##/### p < 0.05/0.01/0.001 between genotypes (same time); */**/*** p < 0.05/0.01/0.001 versus 0′ (same genotype).
Figure 2. Relative mRNA levels of Igfbp3, Igfbp5 and Igfals in the tibia of Pappa2wt/wt and Pappa2ko/ko mice at 0′, 30′, 120′ and 240′ after rmIGF-1 administration in males (A–C) and females (D–F). Data are represented as mean ± S.E.M (n = 6–9/group). Tukey-corrected tests: #/##/### p < 0.05/0.01/0.001 between genotypes (same time); */**/*** p < 0.05/0.01/0.001 versus 0′ (same genotype).
Ijms 22 04048 g002
Ijms 22 04048 g003 550
Figure 3. Relative mRNA levels of Col1a1, osteopotin and osteocalcin in the tibia of Pappa2wt/wt and Pappa2ko/ko mice at 0′, 30′, 120′ and 240′ after rmIGF-1 administration in males (A–C) and females (D–F). Data are represented as mean ± S.E.M (n = 6–9/group). Tukey-corrected tests: #/##/### p < 0.05/0.01/0.001 between genotypes (same time); */**/*** p < 0.05/0.01/0.001 versus 0′ (same genotype).
Figure 3. Relative mRNA levels of Col1a1, osteopotin and osteocalcin in the tibia of Pappa2wt/wt and Pappa2ko/ko mice at 0′, 30′, 120′ and 240′ after rmIGF-1 administration in males (A–C) and females (D–F). Data are represented as mean ± S.E.M (n = 6–9/group). Tukey-corrected tests: #/##/### p < 0.05/0.01/0.001 between genotypes (same time); */**/*** p < 0.05/0.01/0.001 versus 0′ (same genotype).
Ijms 22 04048 g003
Table
Table 1. Auxological parameters of Pappa2wt/wt and Pappa2ko/ko mice (males and females) 1.
Table 1. Auxological parameters of Pappa2wt/wt and Pappa2ko/ko mice (males and females) 1.
wt/wt
Male		ko/ko
Male		wt/wt
Female		ko/ko
Female		Interaction
(Genotype vs. Sex)		GenotypeSex
Body length (cm)16.5 ± 0.1215.8 ± 0.08 ###16.3 ± 0.0715.2 ± 0.16 ***/&&nsF1.91 = 70.78
p < 0.0001		F1.91 = 13.04
p = 0.0005
Femur length (cm)1.61 ± 0.041.25 ± 0.03 ###1.41 ±0.02 ###1.2 ± 0.002 ***F1.24 = 5.46
p = 0.028		F1.24 = 87.42
p < 0.0001		F1.24 = 17.70
p = 0.0003
Tibia length (cm)1.07 ± 0.030.87 ± 0.01 ###1.08 ± 0.030.88 ± 0.02 ***nsF1.24 = 45.82
p < 0.0001		ns
Femur weight (g)0.32 ± 0.020.21 ± 0.004 ###0.21 ± 0.01 ###0.18 ± 0.004F1.24 = 6.93
p = 0.014		F1.24 = 25.01
p < 0.0001		F1.24 = 20.05
p = 0.0002
Tibia weight (g)0.21 ± 0.010.11 ± 0.008 ###0.14 ± 0.007 ###0.08 ± 0.004 ***F1.24 = 7.07
p = 0.013		F1.24 = 93.98
p < 0.0001		F1.24 = 37.49
p < 0.0001
Femur weight/length ratio (g/cm)0.2 ± 0.010.16 ± 0.006 #0.15 ± 0.008 ###0.15 ± 0.003nsnsF1.24 = 10.79
p = 0.0031
Tibia weight/length ratio (g/cm)0.2 ± 0.0150.13 ± 0.005 ###0.13 ± 0.008 ###0.1 ± 0.002 *nsF1.24 = 31.50
p < 0.0001		F1.24 = 31.04
p = 0.0031
Femur/body weight ratio (mg/g)10.12 ± 1.227.09 ± 0.18 #8.39 ± 0.819.22 ± 0.33F1.24 = 6.43
p = 0.018		nsns
Tibia/body weight ratio (mg/g)6.62 ± 0.613.95 ± 0.25 ###5.54 ± 0.364.53 ± 0.31nsF1.24 = 20.22
p = 0.0001		ns
1 Data are represented as mean ± S.E.M. Body length: n = 12–33 (wt/wt male, n = 22; ko/ko male, n = 33; wt/wt female, n = 28; ko/ko female, n = 12). Bone parameters: n = 7/group. Two-way ANOVA and Tukey-corrected tests: #/### p < 0.05/0.001 versus wt/wt males; */*** p < 0.05/0.001 versus wt/wt females; && p < 0.01 versus ko/ko males. ns, not significant.
Table
Table 2. Crystallographic indexes of bone hydroxyapatite-(CaOH) in the femur of Pappa2wt/wt and Pappa2ko/ko mice (males and females) 1.
Table 2. Crystallographic indexes of bone hydroxyapatite-(CaOH) in the femur of Pappa2wt/wt and Pappa2ko/ko mice (males and females) 1.
wt/wt
Male		ko/ko
Male		wt/wt
Female		ko/ko
Female		Interaction
(Genotype vs. Sex)		GenotypeSex
Sample Crystallinity (%)45.40 ± 3.8759.57 ± 2.02 ##60.17 ± 2.77 ##57.85 ± 2.07F1.24 = 8.73
p = 0.0069		F1.24 = 4.51
p = 0.044		F1.24 = 5.47
p = 0.027
Crystallite Size (LVol-IB, nm)36.25 ± 5.7524.86 ± 1.64 #27.36 ± 3.2024.61 ± 2.41nsF1.24 = 3.95
p = 0.05		ns
1 Data are represented as mean ± S.E.M. (n = 7/group). Two-way ANOVA and Tukey-corrected tests: #/## p < 0.05/0.01 versus wt/wt male group. See Figure S1A for representative diffractograms and Table S1 for additional information. ns, not significant.
Table
Table 3. Parameters of bone composition in the femur of Pappa2wt/wt and Pappa2ko/ko mice (males and females) 1.
Table 3. Parameters of bone composition in the femur of Pappa2wt/wt and Pappa2ko/ko mice (males and females) 1.
wt/wt
Male		ko/ko
Male		wt/wt
Female		ko/ko
Female		Interaction
(Genotype vs. Sex)		GenotypeSex
Mineral-to-matrix ratio 22.20 ± 0.082.53 ± 0.01 #2.55 ± 0.11 #2.60 ± 0.04nsF1.24 = 4.97
p = 0.035		F1.24 = 4.26
p = 0.049
Carbonate substitution 30.243 ± 0.0040.222 ± 0.006 ##0.223 ± 0.006 #0.217 ± 0.002nsF1.24 = 6.30
p = 0.019		F1.24 = 6.96
p = 0.014
Mineral crystallinity 40.976 ± 0.0050.957 ± 0.006 #0.956 ± 0.005 #0.956 ± 0.003F1.24 = 4.23
p = 0.05		F1.24 = 4.21
p = 0.05		ns
Collagen maturity 52.044 ± 0.0211.848 ± 0.042 ##1.692 ± 0.047 ###1.373 ± 0.022 ***nsF1.24 = 52.84
p < 0.0001		F1.24 = 136.3
p < 0.0001
1 Data are represented as mean ± S.E.M. (n = 7/group). Two-way ANOVA and Tukey-corrected tests: #/##/### p < 0.05/0.01/0.001 versus wt/wt males; *** p < 0.001 versus wt/wt females. ns, not significant. See Figure S1B,C for representative spectra and Table S2 for additional information. 2 Mineral-to-organic matrix ratio: Amount of mineral (phosphate) per amount of organic matrix (collagen) per volume analyzed. Ratio of phosphate peak area (v1v3PO43−: A900–1200 cm−1) and amide I peak area (vC = O: A1585–1720 cm−1). 3 Carbonate substitution: Relative amount and type of carbonate substitution in the bone mineral apatite lattice. Ratio of carbonate peak area (v2CO32−: A850–890 cm−1) and phosphate peak area (v1v3PO43−: A900–1200 cm−1). 4 Mineral crystallinity: Transformation of non apatitic domains into apatitic ones. Ratio of absorbance height at 1030 cm−1 and 1020 cm−1 after the application of second derivatives. 5 Collagen maturity: A measure of collagen crosslink ratio of pyridinium (an older, trivalent collagen crosslink) at 1660 cm−1 to dehydrodihydroxylysinonorleucine (a younger, divalent, and freshly synthesized collagen crosslink) at 1690 cm−1 after the application of second derivatives.
Table
Table 4. Relative mRNA expression of Igfbp3, Igfbp5 and Igfals in the tibia of Pappa2wt/wt and Pappa2ko/ko mice (males and females) 1.
Table 4. Relative mRNA expression of Igfbp3, Igfbp5 and Igfals in the tibia of Pappa2wt/wt and Pappa2ko/ko mice (males and females) 1.
wt/wt
Male		ko/ko
Male		wt/wt
Female		ko/ko
Female		Interaction
(Genotype vs. Sex)		GenotypeSex
Igfbp31.00 ± 0.291.51 ± 0.270.93 ± 0.192.63 ± 0.51 **nsF1.24 = 10.54
p = 0.003		ns
Igfbp51.00 ± 0.181.23 ± 0.140.63 ± 0.140.93 ± 0.15nsnsF1.24 = 4.64
p = 0.041
Igfals1.00 ± 0.031.06 ± 0.050.53 ± 0.04 ##0.59 ± 0.04 &&nsnsF1.24 = 108.6
p < 0.0001
1 Data are represented as mean ± S.E.M. (n = 7/group). Two-way ANOVA and Tukey-corrected tests: ## p < 0.01 versus wt/wt males; ** p < 0.01 versus wt/wt females; && p < 0.01 versus ko/ko males. Abbreviations: Igfbp3, insulin-like growth factor binding protein 3; Igfbp5, insulin-like growth factor 5, Igfals, insulin-like growth factor acid-labile subunit; ns, not significant.
Table
Table 5. Relative mRNA expression of Col1a1, Osteopontin and Osteocalcin in the tibia of Pappa2wt/wt and Pappa2ko/ko mice (males and females) 1.
Table 5. Relative mRNA expression of Col1a1, Osteopontin and Osteocalcin in the tibia of Pappa2wt/wt and Pappa2ko/ko mice (males and females) 1.
wt/wt
Male		ko/ko
Male		wt/wt
Female		ko/ko
Female		Interaction
(Genotype vs. Sex)		GenotypeSex
Col1a11.00 ± 0.191.13 ± 0.201.25 ± 0.201.65 ± 0.26nsnsns
Osteopontin1.00 ± 0.200.77 ± 0.150.64 ± 0.141.80 ± 0.44 *F1.24 = 6.72
p = 0.016		nsns
Osteocalcin1.00 ± 0.090.90 ± 0.120.79 ± 0.121.56 ± 0.18 **/&F1.24 = 10.15
p = 0.004		F1.24 = 6.16
p = 0.02		ns
1 Data are represented as mean ± S.E.M. (n = 7/group). Two-way ANOVA and Tukey-corrected tests: */** p < 0.05/0.01 versus wt/wt females; & p < 0.05 versus ko/ko males. Abbreviations: Col1a1, collagen, type 1, alpha 1; ns, not significant.
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Rubio, L.; Vargas, A.; Rivera, P.; López-Gambero, A.J.; Tovar, R.; Christians, J.K.; Martín-de-las-Heras, S.; Rodríguez de Fonseca, F.; Chowen, J.A.; Argente, J.; et al. Recombinant IGF-1 Induces Sex-Specific Changes in Bone Composition and Remodeling in Adult Mice with Pappa2 Deficiency. Int. J. Mol. Sci. 2021, 22, 4048. https://doi.org/10.3390/ijms22084048

AMA Style

Rubio L, Vargas A, Rivera P, López-Gambero AJ, Tovar R, Christians JK, Martín-de-las-Heras S, Rodríguez de Fonseca F, Chowen JA, Argente J, et al. Recombinant IGF-1 Induces Sex-Specific Changes in Bone Composition and Remodeling in Adult Mice with Pappa2 Deficiency. International Journal of Molecular Sciences. 2021; 22(8):4048. https://doi.org/10.3390/ijms22084048

Chicago/Turabian Style

Rubio, Leticia, Antonio Vargas, Patricia Rivera, Antonio J. López-Gambero, Rubén Tovar, Julian K. Christians, Stella Martín-de-las-Heras, Fernando Rodríguez de Fonseca, Julie A. Chowen, Jesús Argente, and et al. 2021. "Recombinant IGF-1 Induces Sex-Specific Changes in Bone Composition and Remodeling in Adult Mice with Pappa2 Deficiency" International Journal of Molecular Sciences 22, no. 8: 4048. https://doi.org/10.3390/ijms22084048

APA Style

Rubio, L., Vargas, A., Rivera, P., López-Gambero, A. J., Tovar, R., Christians, J. K., Martín-de-las-Heras, S., Rodríguez de Fonseca, F., Chowen, J. A., Argente, J., & Suárez, J. (2021). Recombinant IGF-1 Induces Sex-Specific Changes in Bone Composition and Remodeling in Adult Mice with Pappa2 Deficiency. International Journal of Molecular Sciences, 22(8), 4048. https://doi.org/10.3390/ijms22084048

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