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Article

Deletion of Diterpenoid Biosynthetic Genes CYP76M7 and CYP76M8 Induces Cell Death and Enhances Bacterial Blight Resistance in Indica Rice ‘9311’

by
Min Jiang
1,2,†,
Ning Yu
1,2,3,†,
Yingxin Zhang
1,2,
Lin Liu
1,2,
Zhi Li
1,2,
Chen Wang
1,2,
Shihua Cheng
1,2,
Liyong Cao
1,2,4,* and
Qunen Liu
1,2,*
1
State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 311400, China
2
Key Laboratory for Zhejiang Super Rice Research, China National Rice Research Institute, Hangzhou 311400, China
3
Guangdong Key Laboratory of New Technology in Rice Breeding, Rice Research Institute of Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
4
Northern Center for China National Rice Research Institute, China National Rice Research Institute, Hangzhou 311400, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2022, 23(13), 7234; https://doi.org/10.3390/ijms23137234
Submission received: 24 May 2022 / Revised: 23 June 2022 / Accepted: 27 June 2022 / Published: 29 June 2022
(This article belongs to the Special Issue Plant Disease Resistance 2.0)
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Abstract

:
Lesion mimic mutants (LMMs) are ideal materials for studying cell death and resistance mechanisms. Here, we identified and mapped a novel rice LMM, g380. The g380 exhibits a spontaneous hypersensitive response-like cell death phenotype accompanied by excessive accumulation of reactive oxygen species (ROS) and upregulated expression of pathogenesis-related genes, as well as enhanced resistance to Xanthomonas oryzae pv. oryzae (Xoo). Using a map-based cloning strategy, a 184,916 bp deletion on chromosome 2 that overlaps with the diterpenoid biosynthetic gene cluster was identified in g380. Accordingly, the content of diterpenoids decreased in g380. In addition, lignin, one of the physical lines of plant defense, was increased in g380. RNA-seq analysis showed 590 significantly differentially expressed genes (DEG) between the wild-type 9311 and g380, 585 of which were upregulated in g380. Upregulated genes in g380 were mainly enriched in the monolignol biosynthesis branches of the phenylpropanoid biosynthesis pathway, the plant–pathogen interaction pathway and the phytoalexin-specialized diterpenoid biosynthesis pathway. Taken together, our results indicate that the diterpenoid biosynthetic gene cluster on chromosome 2 is involved in immune reprogramming, which in turn regulates cell death in rice.

1. Introduction

Plants that spontaneously form necrotic spots on their leaves or stems are called lesion mimic mutants (LMMs) or spotted leaf mutants (SPLs). Due to the phenotype of necrotic spots and the generally concomitant changes in disease resistance, LMMs are ideal materials for deciphering the mechanisms of plant cell death and defense response from the perspective of forwarding genetics. Starting in the early 1980s, LMMs have been found in Arabidopsis, rice, maize, potato, and barley [1]. Studies of these LMMs indicate that the underlying mechanisms of cell death and defense response are complicated. Plant hormones, phytoalexins, and reactive oxygen species (ROS) have nonnegligible roles in plant cell death and defense response. In Arabidopsis mutant atg2-2, spontaneous cell death, early senescence, and disease resistance required the salicylic acid (SA) pathway [2]. In rice LMM lrd6-6, the content of antimicrobial metabolites in leaves, such as diterpenoid phytoalexins and lignin, was significantly higher than that of the wild type, and the resistance to rice blast was enhanced [3]. Defense responses and ROS are induced by the functional inactivation of OsUAP1 in the LMM spl29 [4]. At least 40 LMM genes have been isolated in rice so far.
Phytoalexins are small molecular compounds that can increase plant resistance and can be induced when plants are under external stress. Flavonoids and diterpenoids are the main phytoalexins in rice, and the primary diterpenoids include momilatones, phytocassanes, oryzalexins, and oryzalides. Phytocassanes are essential in resistance to rice blast and sheath blight disease [5]. The biosynthetic genes for phytocassanes are clustered on chromosome 2 (c2BGC) in rice [6]. This diterpenoid biosynthetic gene cluster contains one type II terpene synthase gene (OsCPS2), three type I terpene synthases genes (OsKSL5-7), and six CYP450 family genes (CYP76M5-8, CYP71Z6&7) [6]. OsCPS2 is a phytoalexin-specific ent-copalyl diphosphate (ent-CPP) synthase that converts the common substrate geranylgeranyl pyrophosphate (GGPP) into ent-CPP [7]. OsKSL7 encodes ent-cassa-12,15-diene synthase, which catalyzes the formation of ent-cassa-12,15-diene from ent-CPP, an intermediate product of phytocassane biosynthesis [8]. OsKSL5&6 in indica rice both encode ent-isokaurene synthase, an intermediate product of oryzalides biosynthesis [9]. CYP76M5-8 and CYP71Z6&7 encode hydroxylases that modify the intermediate products of diterpenoid phytoalexin biosynthesis [10]. Another major diterpenoid biosynthetic gene cluster on the rice genome is located on chromosome 4 (c4BGC) and mainly controls momilactone biosynthesis [11]; c4BGC contains OsCPS4, OsKSL4, CYP99A2, and CYP99A3 and a dehydrogenase gene OsMAS [11]. Simultaneous knockout of CYP76M7 and CYP76M8 on c2BGC resulted in a lesion mimic phenotype in rice [12]. Moreover, after knocking out the entire diterpenoid c2BGC, the lesion phenotype persisted; however, in the absence of both gene clusters (c2BGC and c4BGC), the lesion phenotype disappeared [13].
Lignin is a component of the plant secondary cell wall, enhancing mechanical strength and resistance to pathogens [14]. Lignin can be induced by biotic or abiotic stresses, such as wounding and pathogen infection [15]. The structure of lignin is very complex, and it is mainly composed of one or more of the three monolignols p-hydroxyphenyl lignin, guaiacyl lignin, and syringyl lignin [16]. The biosynthesis of lignin occurs through the phenylpropanoid biosynthesis pathway, which also includes flavonoid biosynthesis. Ten key enzymes are involved in biosynthesis from phenylalanine to monolignols: phenylalanine ammonia lyase (PAL), cinnamate 4-hydroxylase (C4H), 4-coumarate: CoA ligase (4CL), hydroxycinnamoyl CoA: shikimate hydroxycinnamoyl transferase (HCT), p-coumaroyl shikimate 3′-hydroxylase (C3′H), caffeoyl CoA O-methyltransferase (CCoAOMT), (hydroxy)cinnamoyl CoA reductase (CCR), ferulate (coniferaldehyde) 5-hydroxylase (F5H), caffeic acid (5-hydroxy-coniferaldehyde) O-methyltransferase (COMT), and (hydroxy)cinnamyl alcohol dehydrogenase (CAD) [15]. Usually, the genes encoding these enzymes have multiple homologs in rice.
In this study, we isolated a new LMM, g380, in the background of indica rice cultivar ‘9311′. Reddish-brown lesions appeared in lower leaves at around 40 days after sowing (DAS). Map-based cloning localized the g380 mutation to a 184,916 bp deletion on the long arm of chromosome 2. The deletion contains most genes of c2BGC. High-level ROS accumulation and the ROS scavenging enzyme system of g380 were disturbed. The defense response was activated prematurely and g380 was more resistant to bacterial blight. RNA-seq results suggested that the phenylpropanoid biosynthesis pathway was upregulated in g380. The upregulated genes in g380 enriched in this pathway are mainly distributed in the monolignol biosynthesis branches. Lignin content in the leaves of g380 was also higher than that of 9311. Pathogenesis-related genes were upregulated supporting spontaneous defense response activation in g380. Our results suggested that the deletion on chromosome 2 induces spontaneous cell death and enhances resistance to bacterial blight in rice.

2. Results

2.1. Characterization of the g380 Mutant

An LMM was isolated from a 60Co γ-ray treated rice mutant library (Oryza sativa L. ssp. Indica cv. ‘9311′) and named g380. There was no difference between g380 and wild-type 9311 plants before the tillering stage, but reddish-brown lesions began to appear on the lower leaves of g380 at the tillering stage (about 40 DAS). Over time the lesions became larger and more oval and spread to all leaves (Figure 1a–c). Compared with 9311, g380 plant height was significantly shorter, the spikelet fertility was lower, and the primary branch number per panicle was higher (Figure 1d–f). No significant differences were observed for the number of panicles per plant, panicle length, or thousand-grain weight of 9311 and g380.
Since leaf chlorophyll directly affects photosynthesis and, therefore, crop growth rate, we determined the chlorophyll content. Chlorophyll a, chlorophyll b, and total chlorophyll were significantly reduced in g380 compared to 9311 (Figure 1g–i). The results suggested that g380 plants have poorer photosynthesis and a lower crop growth rate.

2.2. Fine Mapping of the g380 Locus

To locate g380, we adopted a map-based cloning strategy. First, we found that g380 was located on the long arm of chromosome 2, linked to the markers RM341 and RM3850 through linkage marker screening. Next, we analyzed 104 phenotypic F2 plants and narrowed the range to 2470 kb between C2-9 and C2-10 (Figure 2a). Then, we designed new InDel primers between these two markers based on the polymorphisms of 9311 and Nipponbare genome sequences to continue to analyze 2799 plants with the lesion phenotype in F2 and narrowed the location range to 248 kb between L26 and L39 (Figure 2b,c).
Using chromosome walking, we found that the amplicon with primer L10 could not be formed from the recessive g380 phenotypic plants, which indicates a fragment deletion at the L10 site in g380 (Figure 2e). To confirm the deletion range, ten new primers surrounding L10 (5 kb upstream and 5 kb downstream) were used to amplify 9311 and g380. All ten primers failed to obtain amplicons from g380 but succeeded with 9311 (data not shown), suggesting a large fragment deletion in g380. Additional primers were used to expand the analysis range, leading to identification of the left end of the deletion site between L27 and L28 and the right end of the deletion site between L38 and L39 (Figure 2f). Finally, the deletion site was narrowed to the ranges between F13 and F4 (left), R11 and R10 (right) (Figure 2g). Then, we performed PCR with the forward primer F13 and reverse primer R10, and an amplicon of about 3 kb in size was obtained from g380. The amplicon was sequenced and aligned with the 9311 genome sequence (http://plants.ensembl.org/Oryza_indica/Location/Genome, accessed on 21 June 2022) (Figure 2h).
The result showed a 184,916 bp (location: 2:23,361,216–23,546,131) fragment deletion in g380 relative to the wild-type 9311 (Figure 2c,h). The deletion fragment was aligned to a 224 kb (location: 21,603,461–21,827,464) sequence of the Nipponbare reference genome containing eight annotated genes (Figure 2d). Six of the eight genes belong to the diterpenoid biosynthesis gene cluster on chromosome 2 (c2BGC), which is involved in the biosynthesis of various phytoalexins and functions directly in resistance to rice blast and sheath blight disease [5]. OsKSL7 encodes an ent-cassa-12,15-diene synthase; CYP76M5, CYP76M8, CYP76M7, CYP71Z6, and CYP71Z7 encode putative proteins in the CYP450 family. Of the two remaining genes, CPT1 encodes an NPH3 domain-containing protein, which is a key signal transduction component of higher plant phototropism [17], and LOC_Os02g36000 encodes a putative zinc finger protein. Furthermore, Ye et al. reported that co-knockdown of CYP76M7 and CYP76M8 resulted in a lesion-mimic phenotype in rice [12], and Li et al. found that CYP76M7 and CYP76M8 double knockout mutant cyp76m7/m8 and c2BGC deletion mutant Δc2bgc also have lesion-mimic phenotype, while single knockout mutants of CYP76M7 or CYP76M8 do not show lesion-mimic phenotype [13]. These findings revealed that lesion-mimic phenotype of the g380 mutant comes from the deletion of CYP76M7 and CYP76M8 on c2BGC.

2.3. ROS Accumulation in g380

Reactive oxygen species (ROS) play a vital role in signaling pathways that regulate defense responses in plants. The high accumulation of ROS can trigger redox homeostasis disturbance, which can lead to cell death and, consequently, to limiting biomass and yield production [18]. To ascertain whether the appearance of g380 lesions is related to ROS accumulation, we performed diaminobenzidine (DAB) staining and detected H2O2 content. There was an obvious accumulation of H2O2 around the lesions of g380 leaves (Figure 3a), and total H2O2 in g380 leaves was significantly higher than that of 9311 (Figure 3b).
To further investigate the biochemical mechanisms of ROS accumulation, we measured the activities of catalase (CAT), peroxidase (POD), and total superoxide dismutase (T-SOD). The activity of T-SOD was significantly lower in g380 than in 9311, while CAT activity was significantly increased in g380 compared to 9311, the POD activity had no significant difference between 9311 and g380 (Figure 3c–e). Malondialdehyde (MDA) is produced and accumulated in the cell due to membrane lipid peroxidation. To determine the extent of membrane damage, we measured the content of MDA. MDA content was also significantly increased in g380 (Figure 3f). These results suggested that high-level ROS accumulated in g380 and the ROS scavenging enzyme system of g380 was disturbed.
To explore the order of appearance between ROS accumulation and lesion formation, we performed DAB staining and measured the ROS indexes of g380 and 9311 of 30 DAS when the lesion did not appear in g380. The DAB staining result did not show obvious difference between 9311 and g380. However, CAT and POD activities andH2O2 content increased, T-SOD activity decreased in g380, while MDA content did not show significant difference (Supplementary Figure S1). ROS accumulation appears to precede lesion appearance in g380.

2.4. Activated Defense Response and Increased Resistance to Bacterial Blight in g380

The upregulated expression of some pathogenesis-related (PR) genes, such as OsPR1a, OsPR1b, and OsPR10, is a marker of the defense response activation and can be found in many LMMs [2,3,4]. We measured the transcript levels of OsPR1a, OsPR1b, and OsPR10 by qRT-PCR. The expression levels of these genes were all significantly increased in g380 (Figure 4a–c), suggesting that the defense response of g380 is activated.
To examine whether the activated defense response would lead to enhanced disease resistance, three strains of Xanthomonas oryzae pv. oryzae (Xoo) were tested (Figure 4d,e). The lesion lengths on 9311 leaves were significantly longer than g380 after inoculation, which indicated that g380 exhibited enhanced resistance to Xoo.

2.5. RNA-Seq Data Output and DEG Analysis

After RNA-seq, we obtained 331,699,466 and 312,292,012 raw reads in total from 9311 and g380 samples, respectively. Through screening, we obtained about 309,919,072 (93.43%) and 293,284,164 (93.91%) clean reads from each kind of sample (Supplementary Table S1). Among those clean reads, approximately 235,273,004 (75.91%) from 9311, and 225,019,538 (76.72%) from g380 were mapped to the reference genome. In 9311 and g380, there were 17,829 and 18,582 expressed genes with FPKM values greater than 1 in at least one replicate, respectively. A total of 17,485 genes were expressed in both 9311 and g380, 344 genes were expressed only in 9311, and 1097 genes were expressed only in g380 (Figure 5a).
By analyzing differentially expressed genes (DEGs) with edgeR, we obtained 591 genes that were significantly differentially expressed in 9311 and g380, of which five DEGs were downregulated in g380 relative to 9311. Three of these five downregulated genes were located in the deletion area. The remaining two were putative expressed proteins, and no more detailed annotations were available. So, the subsequent analyses were based on 585 upregulated DEGs.
We selected ten genes for qRT-PCR to validate the reliability of the DEGs. The RT-qPCR results were consistent with the obtained DEGs (Supplementary Figure S2), demonstrating that these DEGs are reliable and can be used for subsequent analysis.
PR genes and WRKY genes are essential for defense responses. Thus, we analyzed the PR genes and WRKY genes in DEGs. The 20 PR genes, which belong to different PR gene families, had higher transcript levels in g380 leaves compared with 9311 (Supplementary Table S2). The 7 WRKY genes showed higher expression levels in g380 (Supplementary Table S3). Among these WRKY genes, WRKY32 has the highest Log2FC value of 4.79.
To check the gene ontology to which the obtained DEGs belong, we performed GO enrichment analysis. A total of 378 DEGs were significantly enriched in molecular function and biological process. In the molecular function ontology, the top three terms were catalytic activity (GO:0003824), oxidoreductase activity (GO:0016491), and iron ion binding (GO:0005506), suggesting that upregulated genes mainly function in redox reactions and iron ion combinations. The top three terms for biological process were oxidation reduction (GO:0055114), carbohydrate metabolic process (GO:0005975), and amine metabolic process (GO:0009308), indicating that upregulated genes are mainly involved in redox reactions, carbohydrate metabolism, and amine metabolism (Figure 5b).
KEGG pathway enrichment analysis was performed to identify the pathways in which those DEGs participate. The result showed that most DEGs that had KEGG annotations belonged to the metabolic pathway and the biosynthesis of secondary metabolites. Furthermore, 109 DEGs were significantly enriched in five pathways: phenylpropanoid biosynthesis (dosa00940), plant–pathogen interaction (dosa04626), diterpenoid biosynthesis (dosa00904), glutathione metabolism (dosa00480), and MAPK signaling pathway (dosa04016) (Figure 5c). Most of the upregulated genes enriched in phenylpropanoid biosynthesis pathway are genes involved in lignin biosynthesis, which are essential in plant resistance (Supplementary Table S4). In the plant–pathogen interaction pathway, the upregulated genes are mainly PR genes (Supplementary Table S5). There are ten upregulated genes and four deleted genes enriched in diterpenoid biosynthesis. Among the ten genes enriched in glutathione metabolism, nine genes encode glutathione S-transferase and the remaining gene encodes ascorbate peroxidase. The MAPK signaling pathway enriched four PR genes, two chitinase genes, OsWRKY24, OsCATA, OsMPK3, and Osrboh7.

2.6. Decreased Diterpenoid Biosynthesis in g380 Leaves

Diterpenoid phytoalexins, such as momilactones and phytocassanes, have a vital role in rice defense [5]. In this study, c2BGC that mainly controls phytocassanes biosynthesis is mostly missing (Figure 2d and Figure 6a) [19]. Moreover, KEGG pathway enrichment analysis suggested that upregulated genes in g380 were significantly enriched in diterpenoid biosynthesis (Figure 5c and Figure 6a) [19]. Since the g380 lesion phenotype was associated with ROS defense signaling molecules (Figure 3), and the diterpenoid phytoalexin was induced by defense responses, we could not determine whether the upregulation of diterpenoid biosynthesis genes is due to the lesion phenotype-related defense response induction.
Based on this, we used qRT-PCR to detect the expression levels of two and three genes (OsCPS2, CYP76M6, and OsCPS4, OsKSL4, CYP99A2) present on chromosomes 2 and 4, respectively, in 9311 and g380, before and after g380 lesion formation (Figure 6b–f). The expression levels of these genes in g380 were significantly upregulated before and after lesion formation. This finding suggested that the upregulation of diterpenoid biosynthesis genes before g380 lesion formation is due to the deletion of the majority of the diterpenoid c2BGC, rather than induced by the lesion phenotype. It is worth noting that the relative expression levels of these diterpenoid biosynthesis genes were significantly higher after the formation of g380 lesions than before. This indicates that the upregulation of diterpenoid biosynthesis genes after lesion formation was mainly caused by the lesion-related defense response.
As mentioned above, g380 has enhanced resistance to bacterial blight. We thus wondered whether the upregulated expression of the diterpenoid biosynthesis genes leads to consistent changes in diterpenoid content in g380 leaves, and thereby enhances g380 disease resistance. To answer this question, we measured the content of three major diterpenoids in 9311 and g380 leaves before and after the lesions formed in g380 (Figure 6g–i). The content of diterpenoid phytoalexins in g380 was less than that in 9311 before and after the formation of g380 lesions. In g380 leaves, phytocassanes cannot be detected before and after the formation of g380 lesions; momilactone A and oryzalexin A and C can be detected after the formation of g380 lesions, but they also cannot be detected before lesions formed. Although lesion formation induced diterpenoid phytoalexins production in g380 leaves, overall, g380 had a lower diterpenoid phytoalexins content than 9311, which would not account for the enhanced resistance after lesion formed in g380.

2.7. Increased Lignin Biosynthesis in g380

Lignin is part of the physical defense of plants against the invasion of pathogens [14]. Through KEGG pathway enrichment analysis, we found that genes in the lignin biosynthesis branch of the phenylpropanoid biosynthesis pathway were significantly up-regulated. This suggested that the lignin biosynthesis pathway has been activated in g380 plants at the transcriptome level. To further confirm this result, we measured the expression levels of some lignin biosynthesis genes, such as OsPAL1, OsPAL4, OsF5H1, OsROMT9, OsCCR20, and OsCAD2, in 9311 and g380 (Figure 7a–f). We also determined the lignin content in flag leaves of 9311 and g380 plants (Figure 7g). The results showed that in the leaves of g380 plants with the lesions, the expression of lignin biosynthesis genes and the content of lignin were significantly higher than those of 9311.

3. Discussion

CYP76M7 and CYP76M8 are located on c2BGC and both encode CYP450 family proteins. It is reported that CYP76M7 protein function as ent-cassadiene C11α-hydroxylase, which play a role in phytocassane biosynthesis [6]. CYP76M8 protein is multifunctional, and redundant with CYP76M5–7 proteins [20]. Ye et al. reported that the simultaneous knockdown of CYP76M7 and CYP76M8 in the background of Nipponbare resulted in the formation of lesions on leaves of adult plants, and the accumulation of 3α-hydroxy-ent-cassadiene and 3α-hydroxy-ent-cassadien-2-one [12]. Therefore, they speculated that the substrates of CYP76M7 and CYP76M8 might be 3α-hydroxy-ent-cassadiene and 3α-hydroxy-ent-cassadien-2-one, and that the formation of lesions might be caused by cytotoxicity of these two phytocassane intermediates [12]. In that study, the formation of lesions was not caused by the pathogen invasion of the mutants, similar to our mutant g380, which can spontaneously form a lesion mimic phenotype. In addition, the deletion fragment of g380 on chromosome 2 contains CYP76M7 and CYP76M8. However, in our study, the type I terpene synthases OsKSL7, which is a key enzyme in the biosynthesis of phytocassane, is also missing. The biosynthesis of precursors of 3α-hydroxy-ent-cassadiene and 3α-hydroxy-ent-cassadien-2-one is blocked, yet the lesion phenotype still exists. It suggested that the formation of lesions is probably not caused by the accumulation of 3α-hydroxy-ent-cassadiene and 3α-hydroxy-ent-cassadien-2-one. This is supported by the research of Li et al. who found that CYP76M7 and CYP76M8 single knockout mutants, c4BGC knockout mutants, and c2BGC and c4BGC double knockout mutants did not show a lesion phenotype, while CYP76M7 and CYP76M8 double knockout mutants and c2BGC knockout mutants showed a lesion phenotype [13]. They speculated that the formation of lesions was related to the accumulation of c4BGC metabolites, which are the substrate of CYP76M7 and CYP76M8. The accumulation of these substrates leads to the appearance and gradual severity of cell death lesions. These results comprehensively identified that the genes responsible for g380 lesion phenotype are CYP76M7 and CYP76M8. Although CYP76M7 and CYP76M8 RNAi mutants, knockout mutants, and g380 all showed lesion phenotype, the first two were more similar, which have smaller and denser lesions than g380. The reason for the difference might be the background― the background of g380 is Indica rice 9311, RNAi mutant has the background of Nipponbare, and knockout mutant has the background of kitaake japonica rice. It also shows that the simultaneous deletion of CYP76M7 and CYP76M8 leads to the emergence of lesion phenotype in both japonica and indica rice. In addition, we found the interesting phenomenon that g380 cultured in greenhouses did not show a lesion phenotype. The biggest difference between our greenhouse and field culture conditions lies in the different spectral components. This shows that spectral components are vital for the generation of the lesions. Chloroplast is the main site of photosynthesis and one of the main sites of ROS production. The production of ROS in chloroplasts is closely related to light-dependent photosynthesis. In our study, ROS of g380 cultured in the field began to accumulate before the appearance of lesions (Supplementary Figure S1). We speculated that ROS accumulation may also be one of the causes of lesion formation. Apart from the aforementioned differences, the variation trends of oryzalexin content in c2BGC knockout mutants and g380 were also inconsistent. In the c2BGC knockout mutants, the total content of oryzalexin A–F was not significantly different from that of the wild-type [13]. In our study, the total amount of oryzalexin A and C showed significant reduction. The cause of this difference remains to be studied.
Most notably, both the diterpenoid c2BGC deletion mutant and g380 had enhanced bacterial blight resistance, upregulated expression of PR genes, and accumulated H2O2 in leaves after the formation of lesions [13]. It seems that the hypersensitive response (HR) was presented in the c2BGC deletion mutant and g380. The HR to a pathogen is one of the most efficient defense mechanisms in nature and leads to the induction of PR genes, ROS burst, and cell death [21]. PR proteins are conserved in higher plants and are also involved in signaling and stress response [22]. They are classified into 17 families, based on the similarity of protein sequence, serology relationship, and enzyme activity [23]. In our study, 20 PR genes belonging to nine families were up-regulated (Supplementary Table S2), and previous studies showed that many were induced after inoculation with Magnaporthe grisea and Xoo. Furthermore, overexpression was demonstrated to enhance the disease resistance for some genes [24,25,26,27]. In addition, our RNA-seq results showed that seven WRKY genes exhibited enhanced transcripts in g380 (Supplementary Table S3). Previous studies have also shown that WRKY genes are involved in the defense against attack from pathogenic bacteria and fungi, and responses to abiotic stress, such as wounding, drought, and cold [28]. Among them, the expression level of WRKY28 can be induced by infection with M. grisea, but it acts as a negative regulator of innate immunity in rice [29]. We also found that the expression levels of PAL1 and OsWRKY19 were increased (Figure 7, Supplementary Table S3). Studies have reported that OsRac1 is a key defensive response regulator, and OsPAL1 and OsWRKY19 are regulated by the downstream transcription factor OsRAI1 of OsRac1 [30].
Lignin is the second most abundant plant biopolymer after cellulose and is mostly deposited in the secondary cell walls of vascular plants [14]. Besides water transport and mechanical support, lignin also aids pathogen defense by accumulating throughout the HR region as a physical barrier against infection by pathogens [31]. Our study found that DEGs involved in the phenylpropanoid biosynthesis pathway mainly participate in the biosynthesis of hydroxyphenyl lignin, guaiacyl lignin, and syringyl lignin, which are the monolignols incorporated into lignin polymers. The lignin content in g380 flag leaves was significantly increased relative to 9311. Increased lignin content may be one of the reasons for the enhanced resistance of g380.
Phenylalanine ammonia lyase (PAL) is the first committed step in monolignol biosynthesis and the phenylpropanoid pathway [15]. Apart from cell wall construction and differentiation, plant peroxidases are also implicated in the defense against pathogens [32]. Studies in tobacco and rice have shown that the expression of PAL genes can affect the plant–pathogen interaction [33,34], and PAL-suppressed tobacco did not establish systemic acquired resistance [35]. Transcription of OsPAL1 and OsPAL4 is much higher in g380 than in 9311.
However, both blast and bacterial blight resistance of the diterpenoid c2BGC deletion mutant were attenuated before lesion formation [13]. Our study also showed that lignin content was lower in g380 before the formation of lesions (Supplementary Figure S3). However, ROS has accumulated before lesion formation. It is possible the defense response has been activated before the appearance of lesions, but not enough to resist the pathogens. At the same time, we also noted that g380 senescence was slower than that of the WT at the filling stage, although higher ROS accumulated in g380, indicating that ROS accumulation did not affect the aging process of g380. This means that as a signal molecule, the accumulation level of ROS is very important to induce rice senescence, or other factors additional to ROS are needed for this process. In addition, according to the “source-sink-transportation” theory, we inferred that the generation of g380 lesion leads to poor nutrient “transportation”, leading to the competition of leaf tissue as a “source” for more nutrients, which results in the greener leaves of g380, and the decrease in the seed setting rate of grains as a “sink”.
In conclusion, we speculated that the large fragment deletion of c2BGC will lead to the activation and runaway of defense response, which will lead to ROS accumulation, the up-regulation of PR gene, cell death and the formation of lesions, the up-regulation of other diterpenoid genes, the increase in lignin biosynthesis, and the enhanced resistance to bacterial blight.

4. Materials and Methods

4.1. Plant Materials and Growing Conditions

The g380 mutant was isolated from a 60Co γ-ray mutant library (Oryza sativa L. ssp. Indica cv. 9311). The newly screened plants with the lesion phenotype were backcrossed with wild-type 9311 and selfed for multiple generations, and the g380 plants with stable lesion phenotype and non-segregated progeny traits were selected as mutant parents. The wild-type 9311 was obtained from the seed bank of our laboratory. G380 was crossed with Nipponbare to obtain an F2 mapping population. All plants were grown in a paddy field under natural conditions at the China National Rice Research Institute, Fuyang, China.

4.2. Measurement of Agronomic Traits and Chlorophyll Content

Twenty individuals were chosen randomly from the wild-type 9311 and g380 and were used to measure agronomic traits. Plant height and panicle length were measured and panicle number per plant, the primary branch number per panicle, spikelet fertility, and thousand-grain weight were calculated manually. Hypothesis testing was performed using a Welch’s t-test with GraphPad Prism version 7.0.0 (for Windows, GraphPad Software, San Diego, CA, USA, www.graphpad.com, accessed on 21 June 2022).
The chlorophyll content was measured by slightly modifying the method of Arnon [36]. The wild-type 9311 and g380 leaf samples used for the determination of chlorophyll content were taken from the flag leaves at about 60 DAS; the g380 flag leaves had lesions at this time. Three biological replicates were sampled, with each biological replicate containing flag leaves from three different individuals. The leaf samples were cut into square pieces with a side length of about 0.2 cm, soaked in 80% acetone solution, protected from light for ten hours, centrifuged, and the supernatant was taken to measure the absorbance at wavelengths of 663 nm and 645 nm. Subsequent chlorophyll content calculations were based on Arnon’s method without modifications. Hypothesis testing was also performed using a Welch’s t-test.

4.3. Linkage Analysis and Chromosome Walking

The F2 mapping population derived from the cross between g380 and Nipponbare was used for linkage analysis. The 128 SSR (simple sequence repeats) and InDel (insertion-deletion) markers from our laboratory that are polymorphic in the 9311 and Nipponbare genomes were selected. Taking the F2 parents (g380 and Nipponbare) as a reference, linkage marker screening was performed on a mixed pool composed of equal concentrations of gDNA of eight individuals with the lesion phenotype in the F2 population. We then genotyped 104 lesion phenotypic individuals in the F2 population using screened linkage markers to complete the primary mapping. The 56 additional InDel markers were designed (and given IDs starting with “L”) in the primary mapping interval based on genome sequence polymorphisms between 9311 and Nipponbare. Seven polymorphic primers were screened and used to genotype 2799 lesion phenotypic individuals in the F2 population. Later, some of these designed markers were also used for chromosome walking to confirm the exact location of the deleted region in g380. Next, we designed PCR primers with an amplicon size of about 1 kb near the markers that we found at both ends of the deletion, which overlap each other to ensure that the endpoints of the deleted region are not missed. These primers were used to check whether any amplicons were generated from g380, to finally determine the deletion site. Finally, the deletion area of g380 was determined by sequencing. The primers used in the linkage analysis and chromosome walking are listed in Supplementary Table S6.

4.4. DAB Staining and ROS Related Physiological Indexes Measurement

DAB staining was performed to detect hydrogen peroxide accumulation [37]. The flag leaves from 9311 and g380 were collected around 60 DAS and immersed in 10% sodium dodecyl sulfate (SDS) solution for about 15 min to remove the wax on the surface of the leaves. Then the leaves were rinsed with water and immersed in 1 g/L DAB dye solution for 6–8 h. Afterward, the leaves were boiled in 95% ethanol until they were completely depigmented. Pictures were taken of the leaves with a scanning imager from Hangzhou Wseen Testing Technology Co., Ltd. (Hangzhou, China).
ROS-related physiological indexes, including H2O2 and MDA contents, and CAT, POD, and T-SOD activities were measured following the instructions of the Nanjing Jiancheng kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China. Code No. A045-2, A064-1, A007-1, A084-3, A001-3, and A003-1). Samples for measurement were collected from flag leaves of 9311 and g380 at about 60 DAS. Six replications (three biological replicates with two technical replicates) were performed for each sample of the five indexes. All data collected were analyzed using a Welch’s t-test in GraphPad Prism 7.0.0 for a difference significance test.

4.5. qRT-PCR Expression Analysis

In this study, most samples for total RNA extraction were collected from flag leaves of 9311 and g380 plants at about 60 DAS. To detect the expression of diterpenoid biosynthesis genes before g380 lesion formation, samples were instead taken of newer g380 and 9311 leaves at about 30 DAS (at this time, no lesion formed on any leaves of g380 plants).
cDNA was synthesized from total RNA using the ReverTra Ace qPCR RT Master Mix with gDNA Remover (Toyobo, Japan, Code No. FSQ-301) according to the manufacturer’s instructions. TB Green-based qRT-PCR reactions (TB Green® Premix Ex Taq™ II, Takara, Japan) were performed in a LightCycler 480 II (Roche, Sweden). Six replications (three biological replicates with two technical replicates) were performed for each sample of each gene. For relative quantification, gene expression was calculated by the 2−ΔΔCt method. The rice Actin gene (LOC_OS03g50885) was used as the internal control for the levels of cDNA used. The difference significance test of data was determined by Welch’s t-test in GraphPad Prism 7.0.0. The primers used for qRT-PCR are listed in Supplementary Table S7.

4.6. Disease Evaluation

Three Xanthomonas oryzae pv. Oryzae (Xoo) strains, Xoo-173, Xoo-339, and Xoo-347, were used to evaluate bacterial blight resistance. The 9311 and g380 plants were grown in the greenhouse at the China National Rice Research Institute, Fuyang, China, and flag leaves were used for inoculation based on the clipping method at around 60 DAS. The lesion length was measured 15 d after inoculation. More than six replications were performed for each sample of the three strains. Welch’s t-test in GraphPad Prism 7.0.0 was conducted on the lesion length data to test the significance of the difference.

4.7. RNA-Seq and Data Analysis

RNA extraction was undertaken on flag leaf tissues from 9311 and g380 plants. Total RNA was extracted and purified using Trizol reagent (Invitrogen, Carlsbad, CA, USA) and RNeasy Plant Mini Kit (Qiagen, Valencia, CA, USA), respectively. After extraction, we used the Nanodrop 2000c to quantify the RNA, and RNA quality was then examined using a Bioanalyzer 2100 (Aligent, Santa Clara, CA, USA). First-strand cDNA was generated using reverse transcriptase and random primers. After synthesis of second-strand cDNA and adaptor ligation, 200 bp cDNA fragments were isolated using gel electrophoresis and PCR amplified by 18 cycles. Library sequencing was undertaken with an Illumina HiSeq2000 instrument.
After obtaining the RNA-seq raw data, high-quality clean data were obtained by removing the adaptor sequences, reads with more than 10% unknown bases (N), and low-quality reads containing more than 50% of bases with Q < 30 and trimming low-quality bases (Q < 30) from the 5’ and 3’ ends. The clean reads were then mapped onto the Nipponbare reference genome (Rice Genome Annotation Project) using Tophat (v 2.0.5). The gene expression levels were quantified in terms of FPKM (fragments per kilobase of exon model per million mapped reads). The R Programming Language and package edgeR [38] were used to perform differential expression analysis with FDR < 0.05 and an estimated absolute log2(FC) > 1.

4.8. GO and KEGG Enrichment Analysis

We used the SEA method of agriGO v2.0 [39] to perform GO (Gene Ontology) enrichment analysis on the obtained up-regulated DEGs. The R Programming Language and clusterProfiler package [40] were used for KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway enrichment analysis. The relevant parameters were organism = ‘dosa’, pAdjustMethod = ‘BH’, qvalueCutoff = 0.05; the rest of the parameters were default. The KEGG pathway map was completed by submitting the data to the KEGG database and using KEGG Mapper.

4.9. Lignin and Diterpenoid Content Measurement

Lignin content was determined using a Lignin Content Assay Kit (Catalog Number AKSU010M) purchased from Beijing Boxbio Science & Technology Co., Ltd. (Beijing, China). The samples used for lignin content determination were taken from 9311 and g380 flag leaves at about 60 DAS. Three biological replicates were taken from 9311 and g380. More than three individual plants were mixed for each biological replicate, and each individual could not be selected repeatedly. Sample treatment and content calculation were carried out according to the instructions of the kit. The data were submitted to Welch’s t-test in GraphPad Prism 7.0.0 for significant difference analysis.
The samples for determination of diterpenoid content were selected from 9311 and g380 plants planted in the same field in two stages with a sowing date interval of about 30 days. Four groups of samples were selected: the newest leaves of g380 without lesions at about 30 DAS and the newest leaves of 9311 in the same period; the flag leaves of g380 with lesions at about 60 DAS and the flag leaves of 9311 in the same period. Three biological replicates were taken for each group of samples, no less than six individual plants were collected for each biological replicate, and each individual plant was selected without repetition. The leaf samples were quickly frozen in liquid nitrogen after being cut off and then lyophilized with a lyophilizer. The lyophilized leaves were ground with a Mixer Mill MM 400 (Retsch). Subsequent sample extraction and LC-ESI-QQQ-MS/MS system analysis were performed according to the method of Chen et al. [41]. The obtained relative contents of the same diterpenoids were accumulated, and Welch’s t-test in GraphPad Prism 7.0.0 was used to verify whether the difference was significant.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/ijms23137234/s1.

Author Contributions

Q.L., Y.Z., L.C. and S.C. conceived the idea and designed the research. M.J., N.Y., Q.L., Y.Z., L.L., Z.L. and C.W. conducted the experiments and analyzed the data. M.J. and N.Y developed the first full draft of the manuscript. Q.L., M.J., L.C. and S.C. revised and critically reviewed the manuscript. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by the National Natural Science Foundation of China, grant numbers 31961143016, 31801726, and 31871236; the Agricultural Science and Technology Innovation Program of the Chinese Academy of the Agricultural Sciences, grant number CAAS-ASTIP-2013-CNRRI; the Fundamental Research Funds of Central Public Welfare Research Institutions, grant number 2017RG001-1; and the Open Project Program of State Key Laboratory of Rice Biology, grant number 20210203.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Acknowledgments

We would like to sincerely thank Dianxing Wu from Zhejiang University for providing valuable materials of rice lesion mimic mutant g380. We are also very grateful to Jianli Wu from the China National Rice Research Institute for providing the Xoo strains. Thanks to Jie Luo and Chuansong Zhan of Hainan University for completing the determination of diterpenoid metabolites. Thanks to Aaron Lorheed Chan for the language revision.

Conflicts of Interest

The authors declare no conflict of interest.

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Figure 1. Characterization of the g380 mutant. (a) Plants of 9311 and g380 at the tillering stage. (b) Plants of 9311 and g380 at the maturity stage. (c) Leaves of 9311 and g380. (d–f) Agronomic traits of 9311 and g380 plants. PH, plant height; SF, spikelet fertility; PBNP, primary branch number per panicle. (g–i) The chlorophyll content of 9311 and g380 leaves. Bar = 10 cm in (a) and (b); red arrows point out lesions on g380 leaves in (a); bar = 1 cm in (c). ** represent p < 0.01 by Welch’s t-test.
Figure 1. Characterization of the g380 mutant. (a) Plants of 9311 and g380 at the tillering stage. (b) Plants of 9311 and g380 at the maturity stage. (c) Leaves of 9311 and g380. (d–f) Agronomic traits of 9311 and g380 plants. PH, plant height; SF, spikelet fertility; PBNP, primary branch number per panicle. (g–i) The chlorophyll content of 9311 and g380 leaves. Bar = 10 cm in (a) and (b); red arrows point out lesions on g380 leaves in (a); bar = 1 cm in (c). ** represent p < 0.01 by Welch’s t-test.
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Figure 2. Fine mapping of g380. (a,b) Preliminary mapping interval. (c) Fine mapping interval; red area represents the deletion fragment. (d) Reference genes in the deletion area. (e–g) Process to determine the deletion area in g380; general view of the process is on the left, and the results of agarose gel electrophoresis are on the right. (h) Results of 9311 and g380 amplified with F13 forward primer and R10 reverse primer; the start and end sequences of the deleted fragments were obtained by sequencing. Red boxes in (e–g) represent the deletion area or primers in the deletion area.
Figure 2. Fine mapping of g380. (a,b) Preliminary mapping interval. (c) Fine mapping interval; red area represents the deletion fragment. (d) Reference genes in the deletion area. (e–g) Process to determine the deletion area in g380; general view of the process is on the left, and the results of agarose gel electrophoresis are on the right. (h) Results of 9311 and g380 amplified with F13 forward primer and R10 reverse primer; the start and end sequences of the deleted fragments were obtained by sequencing. Red boxes in (e–g) represent the deletion area or primers in the deletion area.
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Figure 3. ROS accumulation in g380 after the lesion formation. (a) DAB staining of 9311 and g380 leaves. (b–f) The ROS related physiological–biochemical indexes of 9311 and g380 leaves. Bar = 1 cm in (a). Values are mean ± SD (n = 6). ** indicates significant differences at p < 0.01 level by Welch’s t-test.
Figure 3. ROS accumulation in g380 after the lesion formation. (a) DAB staining of 9311 and g380 leaves. (b–f) The ROS related physiological–biochemical indexes of 9311 and g380 leaves. Bar = 1 cm in (a). Values are mean ± SD (n = 6). ** indicates significant differences at p < 0.01 level by Welch’s t-test.
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Figure 4. The expression levels of PR genes and the result of bacterial blight inoculation. (a–c) The expression levels of PR genes; values are mean ± SD (n ≥ 6). ** indicates significant differences at p < 0.01 level by Welch’s t-test. (d) Phenotypes of representative leaves from the 9311 and g380 plants at 15 d after infection with Xoo-173, Xoo-339, and Xoo-347; bar = 1 cm. (e) Comparison of the lesion lengths on leaves from 9311 and g380 plants at 15 d after infection with Xoo-173, Xoo-339, and Xoo-347 (n ≥ 6); ** means p < 0.01 level by Welch’s t-test.
Figure 4. The expression levels of PR genes and the result of bacterial blight inoculation. (a–c) The expression levels of PR genes; values are mean ± SD (n ≥ 6). ** indicates significant differences at p < 0.01 level by Welch’s t-test. (d) Phenotypes of representative leaves from the 9311 and g380 plants at 15 d after infection with Xoo-173, Xoo-339, and Xoo-347; bar = 1 cm. (e) Comparison of the lesion lengths on leaves from 9311 and g380 plants at 15 d after infection with Xoo-173, Xoo-339, and Xoo-347 (n ≥ 6); ** means p < 0.01 level by Welch’s t-test.
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Figure 5. Expressed gene statistics and GO and KEGG pathway enrichment analysis of DEGs. (a) Venn diagram of 9311 and g380 expressed genes. (b) GO enrichment analysis of upregulated DEGs; the top ten terms enriched in BP and MF. BP: biological process; MF: molecular function. (c) KEGG pathway enrichment analysis of up-regulated DEGs.
Figure 5. Expressed gene statistics and GO and KEGG pathway enrichment analysis of DEGs. (a) Venn diagram of 9311 and g380 expressed genes. (b) GO enrichment analysis of upregulated DEGs; the top ten terms enriched in BP and MF. BP: biological process; MF: molecular function. (c) KEGG pathway enrichment analysis of up-regulated DEGs.
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Figure 6. Decreased diterpenoid biosynthesis in g380 leaves. (a) Biosynthesis process of major diterpenoid phytoalexins in rice; substances in black bordered text boxes are metabolites; genes indicated in blue are deleted in g380, genes indicated in red are DEGs upregulated in g380, genes indicated in black have no significant difference in expression level between 9311 and g380. The solid arrows represent the direct response, and the “solid + dashed” arrows represent that the process has not been explained sufficiently clearly. (b–f) Expression of non-deleted diterpenoid biosynthetic genes in 9311 and g380 before and after the formation of g380 lesions; (b,c) display genes located on chromosome 2; (d–f) display genes located on chromosome 4; error bar represents ± SD; ** indicates p < 0.01 by Welch’s t-test. (g–i) Diterpenoid phytoalexin content in 9311 and g380 leaves before and after g380 lesion formation; error bar represents ± SE; * indicates p < 0.05, ** indicates p < 0.01 by Welch’s t-test.
Figure 6. Decreased diterpenoid biosynthesis in g380 leaves. (a) Biosynthesis process of major diterpenoid phytoalexins in rice; substances in black bordered text boxes are metabolites; genes indicated in blue are deleted in g380, genes indicated in red are DEGs upregulated in g380, genes indicated in black have no significant difference in expression level between 9311 and g380. The solid arrows represent the direct response, and the “solid + dashed” arrows represent that the process has not been explained sufficiently clearly. (b–f) Expression of non-deleted diterpenoid biosynthetic genes in 9311 and g380 before and after the formation of g380 lesions; (b,c) display genes located on chromosome 2; (d–f) display genes located on chromosome 4; error bar represents ± SD; ** indicates p < 0.01 by Welch’s t-test. (g–i) Diterpenoid phytoalexin content in 9311 and g380 leaves before and after g380 lesion formation; error bar represents ± SE; * indicates p < 0.05, ** indicates p < 0.01 by Welch’s t-test.
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Figure 7. Increased lignin biosynthesis in g380. (a–f) The expression levels of lignin biosynthetic genes in 9311 and g380 leaves. (g) Lignin content in 9311 and g380 flag leaves. All data presented as mean value ± SD; hypothesis testing was performed with Welch’s t-test, n = 6, ** means p < 0.01.
Figure 7. Increased lignin biosynthesis in g380. (a–f) The expression levels of lignin biosynthetic genes in 9311 and g380 leaves. (g) Lignin content in 9311 and g380 flag leaves. All data presented as mean value ± SD; hypothesis testing was performed with Welch’s t-test, n = 6, ** means p < 0.01.
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Jiang, M.; Yu, N.; Zhang, Y.; Liu, L.; Li, Z.; Wang, C.; Cheng, S.; Cao, L.; Liu, Q. Deletion of Diterpenoid Biosynthetic Genes CYP76M7 and CYP76M8 Induces Cell Death and Enhances Bacterial Blight Resistance in Indica Rice ‘9311’. Int. J. Mol. Sci. 2022, 23, 7234. https://doi.org/10.3390/ijms23137234

AMA Style

Jiang M, Yu N, Zhang Y, Liu L, Li Z, Wang C, Cheng S, Cao L, Liu Q. Deletion of Diterpenoid Biosynthetic Genes CYP76M7 and CYP76M8 Induces Cell Death and Enhances Bacterial Blight Resistance in Indica Rice ‘9311’. International Journal of Molecular Sciences. 2022; 23(13):7234. https://doi.org/10.3390/ijms23137234

Chicago/Turabian Style

Jiang, Min, Ning Yu, Yingxin Zhang, Lin Liu, Zhi Li, Chen Wang, Shihua Cheng, Liyong Cao, and Qunen Liu. 2022. "Deletion of Diterpenoid Biosynthetic Genes CYP76M7 and CYP76M8 Induces Cell Death and Enhances Bacterial Blight Resistance in Indica Rice ‘9311’" International Journal of Molecular Sciences 23, no. 13: 7234. https://doi.org/10.3390/ijms23137234

APA Style

Jiang, M., Yu, N., Zhang, Y., Liu, L., Li, Z., Wang, C., Cheng, S., Cao, L., & Liu, Q. (2022). Deletion of Diterpenoid Biosynthetic Genes CYP76M7 and CYP76M8 Induces Cell Death and Enhances Bacterial Blight Resistance in Indica Rice ‘9311’. International Journal of Molecular Sciences, 23(13), 7234. https://doi.org/10.3390/ijms23137234

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