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Article

PS II Subunit P in Lilium pumilum (LpPsbP) Confers Saline-Alkali Resistance to the Plant by Scavenging ROS

by
Yibo Jing
1,2,†,
Yu Song
1,2,†,
Shangwei Ji
1,
Ling Zhang
1,
Zongying Wang
1,
Yi Dong
1,2,
Yang Xu
1 and
Shumei Jin
1,*
1
Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin 150000, China
2
Aulin College, Northeast Forestry University, Harbin 150000, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2023, 24(4), 3311; https://doi.org/10.3390/ijms24043311
Submission received: 8 January 2023 / Revised: 27 January 2023 / Accepted: 3 February 2023 / Published: 7 February 2023
(This article belongs to the Special Issue Molecular and Genetic Mechanisms of Plant Stress Response)
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Abstract

:
(1) Background: The growth of plants is impacted by salinity and alkali, Lilium pumilum (L. pumilum) is an ornamental plant with strong resistance to salinity and alkali, while the LpPsbP gene is helpful to fully understand the Saline-Alkali tolerance of L. pumilum. (2) Methods: Gene cloning, bioinformatics analysis, expression of fusion protein, determination of physiological indices of plant after Saline-Alkali stress, yeast two-hybrid screening, luciferase complementation assay, chromosome walking to obtain the promoter sequence, and then analyzed by PlantCARE. (3) Results: The LpPsbP gene was cloned and the fusion protein was purified. The transgenic plants had higher Saline-Alkali resistance than the wild type. A total of eighteen proteins interacting with LpPsbP were screened, and nine sites in the promoter sequence were analyzed. (4) Conclusion: Under Saline-Alkali or oxidative stress, L. pumilum will promote the expression of LpPsbP, which will then directly scavenge reactive oxygen species (ROS) in order to protect its photosystem II, reduce its damage, and thus improve the Saline-Alkali resistance of the plant. Moreover, according to some of the literature and the following experiments, two additional speculations are developed on the mechanisms of how two newly found objects, namely jasmonic acid (JA) and FoxO protein, could be involved in ROS scavenging processes were made.

1. Introduction

Saline-Alkali soil not only restricts plant growth, but also leaves potential hidden risks for maintaining ecosystem stability [1]. In China, the proportion of salinized and alkalized soil areas is relatively high, especially in the northeast. For example, a large proportion of the grassland in the Songnen Plain of northeast China has been salinized and alkalized mainly by sodium carbonate (Na2CO3) and sodium bicarbonate (NaHCO3) [2]. Therefore, it is of great importance to conduct studies on Saline-Alkali-tolerant plants.
Lilium pumilum (L. pumilum) is not only valuable for having the beautiful bright red ornamental flowers, but also for its strong resistance to disease, cold, drought and salinity, which makes it an unparalleled plant material in Saline-Alkali stress research [3,4,5]. Lilium pumilum in this study grows naturally in the heavily salinized and alkalized meadow of Daqing suburb of the Songnen Plain with soil pH as high as 9–10. Exploring the molecular interactions of important Saline-Alkali tolerance genes of Lilium pumilum can provide more theoretical bases for the Saline-Alkali tolerance traits of the plant.
Plants, algae and cyanobacteria all possess photosystem II (PS II) complexes, which have the unique ability to utilize light energy to oxidize water into molecular oxygen. The PS II complex is composed of more than 20 proteins, including intrinsic and extrinsic membrane subunits. On the thylakoid cavity side of PS II, there are three inorganic ion clusters responsible for catalyzing water oxidation, namely manganese (Mn), calcium (Ca) and chloride (Cl) ion clusters (together called Mn4–Ca–Clx cluster). The Mn4–Ca–Clx cluster in PS II is surrounded by several luminal subunits that stabilize these ionic components. Together with the loop regions of several intrinsic membrane subunits, such as D1, D2, CP43 and CP47, these exogenous membrane subunits support the proper assembly and stabilization of the Mn4–Ca–Clx cluster [6]. This complex is called the oxygen evolving complex (OEC), and the luminal subunits of PS II are called the OEC proteins.
The OEC of higher plants consists of three external proteins with apparent molecular weights of 33, 24 and 17 kDa, which are called PsbO (the PS II subunit O), PsbP (the PS II subunit P) and PsbQ (the PS II subunit Q), respectively [7]. These three extrinsic components interact with intrinsic membrane proteins to form a fully functional oxygen-evolving complex. A group of PsbP family proteins was found in the higher plants. This group includes two PsbP proteins (PsbP1 and PsbP2), two PsbP-like proteins (PPL1 and PPL2) and seven PsbP domain proteins (PPD1–PPD7) [8].
The in vivo function of PsbP was first characterized in the green alga Chlamydomonas reinhardtii. A very small amount of PsbP may support the substantial accumulation of PS II complexes, whereas higher concentrations of PsbP in the thylakoid lumen are required to maintain the activeness of manganese ion clusters in vivo [9]. In higher plants, the in vivo function of the PsbP protein was first characterized in transgenic tobacco. PsbP–RNAi plants exhibited growth retardation, light green leaf color and low PS II activity. These studies suggest that PsbP is essential for maintaining the activity of PS II in vivo [10]. Over the last 30 years, a significant amount of work has shown that the PsbP protein can increase the rate of oxygen release at physiological calcium and chloride concentrations in green algae and higher plants [11].
In recent years, there have been numerous studies about the relationship between PsbP protein and stresses. The expression level of PsbP was increased under chilling stress and the application of uniconazole, indicating that low-temperature stress promoted the expression of PsbP [12]. A high-temperature environment greatly inhibited the expression of essential genes involved in the photosynthetic pathway, including PsbP [13]. It was also found that the expression of PsbP was up-regulated under dehydration stress, suggesting that PsbP may play an important functional role in drought tolerance in chickpeas [14]. However, studies directly about the relationship between PsbP protein and Saline-Alkali resistance are still limited. The arbuscular mycorrhizal fungus (AMF) was inoculated into Vaccinium corymbosum, and experiments under alkaline stress were conducted, finding that the increase in PsbP expression in the experimental group promoted the ability of AMF-inoculated plants to resist alkali stress [15].
The principal site of reactive oxygen species (ROS) production in chloroplast thylakoids is the reaction center of PS II [16]. The control of production and removal of ROS in chloroplasts is of great importance in promoting the plants’ salinity tolerance. The PsbP protein plays an important role in maintaining the activity of PS II to release oxygen.
L. pumilum is a Saline-Alkali-tolerant and adaptable plant. Considering all the reviewed literature, information and real situations above, we made a hypothesis according to which the LpPsbP gene is related to the Saline-Alkali resistance and ROS removal of L. pumilum, so the LpPsbP protein may be involved in the regulatory pathway of the Saline-Alkali resistance of L. pumilum by scavenging ROS. In this study, the LpPsbP gene was cloned and its relationship with other species was analyzed using bioinformatics methods. LpPsbP was transferred into tobacco to observe the stresses tolerance, LpPsbP interacting proteins were screened by yeast two-hybrid and the promoter of LpPsbP was analyzed using PlantCARE. The results show that both the experimental conclusions drawn under Saline-Alkali stress conditions and the properties of the screened interacting proteins were related to ROS. Finally, the hypothesis according to which LpPsbP can confer Saline-Alkali resistance to the plants by scavenging ROS was proved. Moreover, according to some studies and the following experiments, two additional speculations on the mechanism of how two newly found two objects, namely jasmonic acid (JA) and FoxO protein, could be involved in ROS scavenging processes were made. This paper attempts to investigate the relationship between LpPsbP and Saline-Alkali stresses by means of molecular biology, genetics and plant physiology methods. It lays a foundation for revealing the molecular mechanism and functional analysis of the L. pumilum gene family in response to Saline-Alkali stress, and has certain scientific significance for making full use of Saline-Alkali soil resources.

2. Results

2.1. Cloning of Open Reading Frame of LpPsbP Gene

Using L. pumilum cDNA as a template and LpPsbP-F and LpPsbP-R as primers (shown in Table 1), a DNA band with the size of about 800 bp was obtained by PCR, and the result of sequencing from a biotechnology company was 795 bp.

2.2. Genetic Relationship between PsbP of L.pumilum and PsbP of Other Species

The amino acid sequence of LpPsbP was predicted by conserved domains using NCBI, finding that LpPsbP has a highly conserved DcrB domain at amino acid positions 1-792, which belongs to the DcrB superfamily (Figure 1A).
By BLAST analysis in the NCBI database, the amino acid sequence of PsbP in L. pumilum has a high similarity with that of other plants. DNAMAN software was used to compare the amino acid sequence of the PsbP cloned from L. pumilum with that of PsbP of other species, finding that the amino acid sequence of the PsbP protein of L. pumilum had a high homology with that of other plants (Figure 1B). Therefore, the gene cloned from L. pumilum cDNA was considered as PsbP and named as LpPsbP.
Based on the comparison of amino acid sequences and phylogenetic relationships, the phylogenetic tree was constructed using the MEGA_64 program, which shows that the PsbP protein is closely related to the PsbP of A. officinalis and F. agrestis (Figure 1C).

2.3. Expression Characteristics of LpPsbP Gene in the Leaves of L. pumilum after Stress Treatment

After different times of H2O2 stress, the expression of LpPsbP in the leaves of L. pumilum was up-regulated about 1.5 times at 6 h, decreased slightly at 12 h and then continued to rise, until the expression reached the peak value of 3.05 times at 48 h (Figure 2A).
After NaCl stress for different times, the expression of LpPsbP in the leaves of L. pumilum increased continuously at first, then decreased dramatically and then surged to the maximum before dropping to the lowest, but the expression was still slightly higher than that in the control group. The expression of the LpPsbP gene peaked at 3.93 times after 36 h of NaCl treatment (Figure 2B).
After different times of NaHCO3 stress, the expression of LpPsbP in the leaves of L. pumilum climbed first and then decreased with the increase in treatment time. The LpPsbP gene expression peaked at 4.38 times after 12 h of NaHCO3 stress (Figure 2C).
At different times of Na2CO3 stress, the expression of LpPsbP in the leaves of L. pumilum raised continuously with the increase in treatment time. The expression of LpPsbP reached a peak value of 4.33 times at 48 h after Na2CO₃ treatment (Figure 2D).

2.4. Optimization Conditions for Induction and Purification of Recombinant pGEX-LpPsbP

After pGEX-LpPsbP was transferred into BL21-competent cells, the activated bacterial solution was added with 0 mM, 0.5 mM, 1 mM, 1.5 mM and 2 mM of isopropyl-beta-D-thiogalactopyranoside (IPTG), and induced at 37℃ for 5 h. The LpPsbP protein first increased at 0.5 mM IPTG. After 1 mM IPTG, the expression of LpPsbP tended to be stable and did not increase (Figure 3A).
Another seven activated bacterial solutions were added with 1 mM IPTG and induced at 37℃ for 0 h, 0.5 h, 1 h, 2 h, 3 h, 4 h and 5 h. The LpPsbP began to express after 0.5 h of induction and the expression level reached the maximum value 1 h after induction (Figure 3B).
In order to obtain the high purity protein of pGEX-LpPsbP, the lysed protein was purified in a purification column with glutathione S-transferase (GST) purification resin. After 1 h of gentle shaking, the filtrate “pGEX-LpPsbP refolding protein” was obtained and eluted twice with protein cleaning buffer. Then, “pGEX-LpPsbP purified protein” was obtained by eluting the protein eluent with buffering solution twice. Finally, the result of SDS-PAGE showed that the target band was successfully purified (Figure 3C).

2.5. Resistance Analysis of Bacterial Solution Expressing LpPsbP Protein under Different Saline-Alkali Stresses

The overexpression of the LpPsbP protein promoted bacterial growth in the solution under different Saline-Alkali stresses. Under normal culture conditions, the optical density in 600 nm (OD600) values of bacterial solution after 5 h of induction using pGEX and LpPsbP were both 1.94 (Figure 3D(a)). In the presence of 0.2 mM NaHCO3, the OD600 values of bacterial solution after 5 h of induction using pGEX and LpPsbP were 1.10 and 1.95, respectively (Figure 3D(b)). Under the treatment of 0.1 mM Na2CO3, the OD600 values of bacterial solution after 5 h of induction using pGEX and LpPsbP were 0.96 and 1.47, respectively (Figure 3D(c)). In the presence of 0.8 mM NaCl, the OD600 values of bacterial solution after 5 h of induction with pGEX and LpPsbP were 1.03 and 1.65, respectively (Figure 3D(d)).

2.6. Gene Expression Analysis of pBI121-LpPsbP Overexpressing Tobaccos

The successfully constructed pBI121-LpPsbP plant expression vector was transformed into tobacco plants. The gene expression of LpPsbP transgenic tobacco was detected by qPCR. As shown in Figure 4A, the expression of LpPsbP in #1-#7 was higher than that in the wild type (WT), among which #1, #4 and #7 had higher expression levels, which were 23.21, 20.20 and 17.7 times those of the WT, respectively. Therefore, the #1, #4 and #7 plants were selected for subsequent experiments.

2.7. Phenotypic Analysis of Tobacco with pBI121-LpPsbP Overexpression under Saline-Alkali Stress

2.7.1. Effect of Salt Stress on Seed Germination

In order to investigate the effects of salt stress on the germination of WT seeds and LpPsbP transgenic seeds, wild-type and LpPsbP transgenic T3 generation tobacco seeds were taken and cultured in 1/2 MS medium and 1/2 MS medium supplemented with 4 mM H2O2, 125 mM NaCl, 8 mM NaHCO3 and 8 mM Na2CO3, respectively, for 7 days. The observation results are shown in Figure 4B. All seeds germinated in 1/2 MS medium, and the seedlings were green. There was no significant difference between wild-type and LpPsbP transgenes visually. Under salt stress of 4 mM H2O2, 125 mM NaCl, 8 mM NaHCO3 or 8 mM Na2CO3, the germination of wild-type seeds was significantly inhibited and most seeds did not germinate, while the young seedlings of germinated seeds were small and yellow in color. All the transgenic seeds germinated under salt stress, and the seedlings were green and grew larger, which was better than the wild type. These results indicated that LpPsbP transgenic seeds had greater tolerance to salt stress during germination.

2.7.2. Analysis of Seedling Resistance of Transgenic Plants under Salt Stress

In order to investigate the resistance analysis of transgenic plants under salt stress, wild-type tobacco seeds and T3 generation LpPsbP transgenic plants were sown separately in the control group, groups in 1/2 MS medium supplemented with 3 mM H2O2, 125 mM NaCl, 4 mM NaHCO3 and 4 mM Na2CO3. The results obtained after 14 days are shown in Figure 4C. In the control medium, wild-type and transgenic seedlings presented green leaves, with long roots and uniform leaves, and there was no significant difference in growth potential between wild-type and transgenic seedlings. In 3 mM H2O2 medium, wild-type seedlings grew less than transgenic seedlings, and all seedlings had shorter roots. In 125 mM NaCl medium, wild-type and transgenic seedlings had the same growth condition of leaves, and the root length was smaller than that of transgenic seedlings. In 4 mM NaHCO3 medium, both wild-type seedlings and transgenic seedlings were yellow, and the root length of the wild-type seedlings was still smaller than that of the wild-type seedlings. In 4 mM Na2CO3 medium, the leaves of the wild-type seedlings and transgenic seedlings developed less, and the leaves of wild-type leaves were completely yellow and transparent, while some parts of the LpPsbP transgenic leaves remained green. The results show that the LpPsbP gene affected seedlings under salt stress and enhanced their resistance to salt stress.

2.7.3. Resistance Analysis of Transgenic Plants under Salt Stress

In order to study the resistance analysis of transgenic plants under salt stress, WT and transgenic tobacco plants with the same growth size in the pot were selected and irrigated with 20 mL of 1 M H2O2, 1 M NaCl, 0.5 M NaHCO3 and 0.5 M Na2CO3 solutions for 7 days. The growth phenotype (Figure 4D) was observed. Under 1 M H2O2 treatment, wild-type plants appeared to bend, while transgenic plants remained upright. Under the treatment of 1 M NaCl and 0.5 M NaHCO3, the plants had no tendency to bend but compared with the transgenic plants, the first to the fourth leaves of WT plants were drooping. Under 0.5 M Na2CO3 treatment, the leaves of wild-type and transgenic plants were drooping from bottom to top, compared with that of wild transgenic plants, which also demonstrated stem bending. The results show that LpPsbP transgenic plants were more tolerant to salt stress than WT plants.

2.7.4. Stomatal Opening in WT and LpPsbP Transgenic Plants after Salt Stress

The stomatal opening and closing of WT and LpPsbP transgenic plants after salt stresses were observed under a microscope. There is no significant difference in stomatal size between WT plants and transgenic plants without salt stress treatment (Figure 4E). After treatment with 4 mM H2O2, 125 mM NaCl, 8 mM NaHCO3 and 8 mM Na2CO3, the WT plants exhibited visible stomatal closure, while the LpPsbP transgenic plants only exhibited slight stomata closure. These results indicate that the WT plants were more sensitive to stomatal switching after salt stress than transgenic plants.

2.7.5. Assessment of O2− and H2O2 Accumulation in Tobacco by NBT and DAB Staining

In order to further verify the content of O2− and H2O2 in tobacco plants, nitro-blue tetrazolium (NBT) and diaminobenzidine (DAB) staining methods were utilized to detect O2− and H2O2 in seedling leaves in situ, so as to understand the ability of LpPsbP to reduce ROS content in plants. The deeper the blue color is, the more O2− is accumulated. The darker the brown color is, the more H2O2 is accumulated (Figure 4F). The results illustrate that the staining results of NBT and DAB show no significant difference between wild-type and transgenic plants under the control condition. The staining deepened after salt stress treatment, and the staining of wild-type leaves was deeper than that of transgenic leaves, indicating that the overexpression of the LpPsbP gene can reduce the accumulation of ROS in plants under salt stress. The LpPsbP gene can enhance plant resistance under salt stress.

2.8. Analysis of Physiological Indices of Tobacco under Saline-Alkali Stress

2.8.1. Relationship between LpPsbP Gene and Gas Exchange Parameters in Tobacco Leaves

In order to explore an association between the LpPsbP gene and leaf gas exchange parameters in tobacco, a LI-6400 photosynthometer was used to measure the net photosynthetic rate (Pn), stomatal conductance (Gs), intercellular CO2 (Ci) and transpiration rate (Tr) of wild-type and LpPsbP transgenic tobacco under different salt treatments (1 M H2O2, 1 M NaCl, 0.5 M NaHCO3 and 0.5 M Na2CO3). There was no significant difference in gas exchange parameters between wild-type and transgenic lines under normal conditions. Under salt stress processing, wild-type lines and transgenic lines in tobacco leaf net photosynthetic rate, transpiration rate and stomatal conductance, as well as intercellular CO2, decreased, but these various parameters of transgenic lines are higher than those of the wild type (Figure 5A). Thus, the overexpression of the LpPsbP gene can effectively alleviate the reduction in gas exchange function in leaves induced by salt stress.

2.8.2. Measurement of Chlorophyll Content of Tobacco under Different Salt Stresses

The chlorophyll contents of tobacco under different salt stresses were measured by a SPAD chlorophyll meter (Figure 5B). The chlorophyll content of wild-type and transgenic tobacco plants in the blank control group was similar when the leaves of the same part were taken from top to bottom. The chlorophyll content of plants was significantly decreased after salt treatment, but the chlorophyll content of transgenic plants was higher than that of the wild-type plants under different stresses. LpPsbP-overexpressing plants had higher salinity tolerance than wild-type plants.

2.8.3. Measurement of O2− Content in Transgenic Plants

Salt stress can lead to the accumulation of O2− in plants and oxidative damage. The ability of LpPsbP to reduce ROS content in tobacco was estimated by measuring O2− content in transgenic plants. Under normal conditions, O2− content in normal tobacco and transgenic tobacco did not change significantly. After salt stress, O2− content in tobacco leaves began to differ. After salt stress, the O2− content in leaves increased significantly, while the O2− content in transgenic lines was lower than that of wild-type lines (Figure 5C), which was consistent with the results of NBT staining for superoxide anion (Figure 4F). These results indicate that the presence of the LpPsbP gene inhibit the accumulation of superoxide anion in tobacco leaves.

2.8.4. Measurement of H2O2 Content in Transgenic Plants

In order to determine the effect of the LpPsbP gene on the accumulation of reactive oxygen species under salt stress, the H2O2 content of wild-type and transgene plants under controlled and salt stress was measured using an H2O2 kit (Grace, Suzhou, China) (Figure 5D). The content of H2O2 in wild-type and transgenic lines is similar without salt stress, and they are both low. The content of H2O2 in the plants under salt stresses, and the content of H2O2 in the wild-type lines was higher than that in the transgenic lines, which was consistent with the result of DAB staining. These results indicate that the wild-type lines accumulated more H2O2 under salt stress and were more sensitive to salt stress. The transgenic plants accumulated less H2O2 and were resistant to salt stress.

2.8.5. Measurement of Malondialdehyde Content Produced by Different Tobacco Lines under Salt Stress

In order to determine the association between high ROS accumulation and cell damage, we examined the malondialdehyde (MDA) content produced by different tobacco lines under salt stress (Figure 5E). The wild-type lines with higher ROS levels accumulate more MDA, while transgenic trains with lower ROS content also had lower MDA expressions. The salt stresses can cause a large accumulation of reactive oxygen species in plants, and the presence of the LpPsbP gene can reduce the accumulation of reactive oxygen species and reduce plant damage.

2.9. Screening and Cotransformation Validation of cDNA Libraries

pGBKT7-LpPsbP was successfully constructed by using two restriction sites of BamH1 and Sal1. The transformed pGBKT7-LpPsbP was fused with the cDNA library of L. pumilum, and the DNA of 19 blue strains was extracted for PCR detection. The results show that only No. 5 had no evident band, and the remaining 18 samples were all given a single band, which was sequenced. The homology of these sequences was outputted in NCBI. The repeated sequences were removed by comparison, and five sequences related to Saline-Alkali resistance were obtained. The protein codes are listed in Table 2.
A total of five successfully compared plasmids related to salinity stress and pGBKT7-LpPsbP plasmid were cotransformed into Y2H and cultured on SD-Trp-Leu-His plus X-α-gal medium at 30℃. The plasmid 2 and plasmid 5-transformed yeasts grew as colonies on the medium and turned blue, while plasmids 1, 3 and 4 did not turn blue. It can be preliminarily concluded that No. 2 and No. 5 interact with pGBKT7-LpPsbP (Figure 6A). In this experiment, the No. 2 LpFoxO gene was selected for subsequent cloning and verification.
For further verification, pGADT7 and LpFoxO plasmids with added restriction site were digested with BamH1 and Xho1, and pGADT7-LpFoxO was successfully constructed. The strains cotransformed with pGBKT7-LpPsbP and pGADT7-LpFoxO could grow blue colonies on SD-Trp-Leu-His plus X-α-gal medium, while there were no blue colonies growth in the control group (Figure 6B). These results indicate that LpPsbP and LpFoxO proteins can interact with each other.

2.10. Luciferase Complementation Assay

After the control groups (functional fragment of N-terminus luciferase, NLuc and C-terminus luciferase, CLuc empty load, NLuc empty load and CLuc empty load) and the experimental group (pCAMBIA1300-NLuc-PsbP and pCAMBIA1300-CLuc-FoxO) were transferred into Agrobacterium tumefaciens GV1301, they were then successively transferred into four quadrants of the same leaf (I, II, III, IV), in which the experimental group was located in the fourth quadrant. As shown in Figure 6C, the experimental group emits visible fluorescence, indicating that PsbP interacts with FoxO.

2.11. Analysis of LpPsbP Promoter

Through three rounds of PCR amplification by the chromosome walking method, a 680 bp fragment was cloned. The cis-acting element of the LpPsbP promoter sequence was analyzed using PlantCARE (shown in Table 3). Then, the TBtools software (Guangzhou, China) was used to carry out a visual analysis of the obtained promoter sequences (Figure 7). The analysis of sequence indicated that this sequence contained multiple regulatory and responsive sites.

3. Discussion

There are many stress factors affecting plant growth and survival, among which Saline-Alkali stress is one of the main abiotic stresses. Saline-Alkali stress can lead to the accumulation of ROS, destroy enzymes in cells and cause oxidative damage to membranes [22]. The PS II subunit P (PsbP) protein is a major component of the oxygen evolution complex (OEC) of chloroplast PS II. The function of the PsbP protein was first proved in transgenic tobacco. RNAi-inhibited PsbP mutants exhibited a severe growth inhibitory phenotype in tobacco, suggesting that PsbP is essential for maintaining PS II activity in vivo [10]. At present, there are many studies on the structural and functional characteristics of plant PsbP, but there are a few studies on the molecular response of PsbP involved in plant stress. The LpPsbP gene was cloned from L. pumilum. The amino acid sequence alignment between L. pumilum and other species showed that it conserved between L. pumilum and other species up to 80%. The PsbP protein was closely related to plants such as F. agrestis by constructing an evolutionary tree.
There were many studies indicating that Saline-Alkali stress can increase the expression of PsbP. For instance, there was the accumulation in PsbP protein and stronger secondary metabolites biosynthesis ability in plants under high-pH stress [15]. IbPsbP was up-regulated 2.6- and 6-fold by 24 h NaCl treatment and H2O2 treatment, respectively [23].
The expression of the LpPsbP gene was significantly up-regulated in the transcriptome of leaves in L. pumilum treated with Saline-Alkali stress. In order to observe whether LpPsbP expression was changed with Saline-Alkali stress or not, L. pumilum was treated with different stresses (H2O2, NaCl, NaHCO3 and Na2CO3). The results show that the expression of the LpPsbP gene changed as treatments started. After being given 48 h of H2O2, 36 h of NaCl, 12 h of NaHCO3 and 48 h of Na2CO3 treatment, the expression levels of the LpPsbP gene reached the maximum. This showed that LpPsbP may play an important role in regulating the response to abiotic stress in L. pumilum.
The LpPsbP–GST fusion protein displayed higher tolerance than the GST protein in E. coli. The germination rate of the transgenic plant was significantly higher than that of the wild-type under Saline-Alkali stress, which indicates that the presence of LpPsbP alleviated the damage of stress. Under Saline-Alkali stress, the growth and seedling formation of transgenic seedlings are relatively less inhibited. The physiological parameters, such as net photosynthetic rate (Pn), stomatal conductance (Gs), intercellular CO2 (Ci), transpiration rate (Tr) and chlorophyll content were measured, finding that the tolerances of transgenic lines were higher than those of wild types.
The PsbP was found to be one of the proteins involved in the scavenging mechanisms of reactive oxygen species (ROS) in plants [24]. RXLR31154 was reported to reduce the accumulation of H2O2 by stabilizing PsbP [25]. PsbP Domain Protein 5 (PPD5) can regulate drought resistance by modulating the accumulation of H2O2 in guard cells [26]. In order to know whether the higher tolerance of the transgenic lines than that of the wild type was caused by the clearance of ROS, the contents of O2−, H2O2 and malondialdehyde (MDA) of wild-type and transgenic lines under salt stress were analyzed. The analysis of the results (NBT and DAB staining, O2−, H2O2 and MDA content in the leaves of LpPsbP-overexpressing tobaccos) showed that the accumulation of O2− and H2O2 in the leaves of LpPsbP-overexpressing plants was lesser than that of wild-type plants. Therefore, it is speculated that the overexpression of the LpPsbP gene would enhance the scavenging of reactive oxygen species in plants.
A total of 18 proteins interacting with LpPsbP were screened by the method of yeast two-hybrid. FoxO belongs to forkheadbox (Fox) protein family, a class of transcription factors with wing-like helix structure in the DNA binding region [24]. FoxO is an important regulator of various intracellular processes. Studies have shown that ROS can regulate the activation and gene expression of the O subgroup of the forkhead box transcription factor family at multiple levels [27]. In some cell types (for example, hematopoietic stem cells), ROS was cleared by upregulating the expression of antioxidant enzymes by the forkhead box O (FoxO) family of transcription factors [28]. Moreover, through the transcriptional activation of genes producing ROS-scavenging proteins such as MnSOD and catalase, FoxO proteins also control aging and cellular senescence [29]. In another study, it is found that KDM5 modulates ROS through the interaction with FoxO [30]. Based on this, we speculate that LpPsbP can interact with FoxO to regulate ROS content.
Additionally, the chromosomal walking method was used to clone the LpPsbP promoter gene. Following analysis, we discovered that the promoter sequence had cis-acting elements in response to abiotic stresses (such as anaerobic condition), light, hormones (such as abscisic acid), etc. Additionally, there were two sites involved in the response of methyl jasmonate (MeJA). Signaling pathways of jasmonic acid are related to ROS wave, and specifically, the role of JA mainly involved in suppressing the ROS wave during responses to high light stress or wounding [31]. Therefore, the promoter of the LpPsbP can be involved in a variety of biological metabolic pathways.

4. Materials and Methods

4.1. Cloning of LpPsbP Gene

Trizol method was used to extract total RNA from L. pumilum, and the full-length cDNA was obtained by Takara reverse transcription kit (Tokyo, Japan). According to the transcriptome sequencing results of L. pumilum bulbs, the open reading frame (ORF) of LpPsbP gene sequence was found and SnapGene software (Boston, MA, USA) was used to design the specific primers. All sequences of the primers used in this paper are shown in Table 1. The target gene was amplified by PCR using gene-specific primers (LpPsbP-F and LpPsbP-R, shown in Table 1) using cDNA as a template. PCR products were sent to Kumei Biotechnology Co., LTD (Changchun, China) for sequencing.

4.2. Bioinformatics Analysis

BLAST analysis of LpPsbP was performed on the NCBI website to find other PsbPs with high homology to LpPsbP in other species. The homologous amino acid sequences of proteins were aligned using DNAMAN software (San Ramon, CA, USA), and the phylogenetic tree was constructed using MEGA_64 software to observe the relationship between PsbP of L. pumilum and PsbPs of other species. NCBI was used to analyze the conserved domain of PsbP.

4.3. Investigating the Expression Characteristics of LpPsbP Gene in the Leaves of L. pumilum after Stress Treatment

The tissue-cultured L. pumilum was transferred to MS medium (pH 5.8) without any stress treatment (CK) and MS medium containing 200 mM NaCl, 20 mM NaHCO3, 11 mM H2O2 and 20 mM Na2CO3. RNA was extracted from leaves after 0 h, 6 h, 12 h, 24 h, 36 h and 48 h of different stresses; then, they were reversely transcribed into cDNA, and the expression of LpPsbP gene in L. pumilum leaves after stress treatment was analyzed by qRT-PCR using the designed primers (LpPsbP qPCR-F and LpPsbP qPCR-R, shown in Table 1).

4.4. Construction of LpPsbP Protein Fusion Expression Vector and Optimization of Induced Expression Conditions

Primers with BamH1 and Xho1 double restriction sites (LpPsbP-BamH1-F and LpPsbP-Xho1-R, shown in Table 1) were designed to construct pGEX-LpPsbP prokaryotic expression vector. The successfully constructed plasmid was transferred into protein expressing strain BL21. pGEX-LpPsbP fusion protein was induced by IPTG and the expression conditions were optimized; then, the fusion protein of pGEX-LpPsbP was purified.
E. coli strain expressing empty vector pGEX was used as a control to compare the growth trend of strains expressing pGEX-LpPsbP protein under different stress conditions. When the OD600 values of the bacterial solution reached about 0.5, different stresses (0.2 mM NaHCO3, 0.1 mM Na2CO3 and 0.8 mM NaCl) were added for different times, and the new OD600 values of the bacterial solution expressing pGEX and pGEX-LpPsbP were measured.

4.5. Resistance Analysis of LpPsbP Transgenic Tobacco under Saline Stress

The plant expression vector pBI121-LpPsbP was constructed using BamH1 and Xho1. The successfully constructed plasmid was transformed into A. tumefaciens-competent cells EH105 by electroshock method, and then pBI121-LpPsbP transgenic tobacco was transformed. The co-cultured leaves were transferred to the screening medium, and seedlings were generated. Finally, DNA of transgenic tobacco seedlings was extracted for PCR identification. After identification, RNA in the leaves of wild-type and transgenic tobacco plants were extracted and reverse-transcribed into cDNA. Finally, qPCR primers were used to detect the expression of transgenic plants.
The seeds of wild-type and T3 LpPsbP overexpressed tobacco were sown in the control medium supplemented with 125 mM NaCl, 8 mM Na2CO3, 8 mM NaHCO3 and 4 mM H2O2 at the same time and cultured for 7 days to observe the germination of seeds. Three groups of repeated biological experiments were performed.
The T3 seeds of wild-type and LpPsbP transgenic lines were simultaneously seeded in the control medium supplemented with 125 mM NaCl, 4 mM Na2CO3, 4 mM NaHCO3 and 3 mM H2O2, and the plates were placed vertically for the growth of the roots. Thus, the roots were allowed to grow vertically for 14 days to observe the growth phenotype of seedlings at this time. Three groups of repeated biological experiments were performed.
Wild-type and LpPsbP transgenic tobacco cultivated in soil with similar size and same growth conditions were selected, respectively. The growth phenotype was observed by watering with 20 mL of 1 M NaCl, 0.5 M Na2CO3, 0.5 M NaHCO3 and 1 M H2O2 solutions for 7 days. Three groups of repeated biological experiments were performed.

4.6. Different Indices of Tobacco with pBI121-LpPsbP Overexpression under Saline-Alkali Stress

The stomata of the same part of the tobacco leaves (WT and LpPsbP#1) under 125 mM NaCl, 8 mM Na2CO3, 8 mM NaHCO3 and 4 mM H2O2 were observed in microscope (Olympus). The leaves of the same part of the sample tested were selected, net photosynthetic rate (Pn), stomatal conductance (Gs), intercellular CO2 (Ci) and transpiration rate (Tr) were measured on the third leaf from top to bottom using LI-6400 photosynthetic apparatus. The light intensity of the instrument was set to 1000 μmol m−2s−1, the gas flow rate was set to 500 μmol s−1 and the CO2 concentration was set to 400 μmol mol−1. The chlorophyll content of tobacco was measured by SPAD chlorophyll meter (Tokyo, Japan). The method of O2− free radical scavenging ability determination refers to [32].
NBT and DAB staining kit was purchased from Tiangen Biotech co., LTD (Beijing, China). The protocol follows the instructions of the kit.
Hydrogen peroxide (H2O2) kit was purchased from Grace Biotechnology co., LTD (Suzhou, China). The UV absorption value was measured at 405 nm according to the specific experimental operation method, and the H2O2 content was calculated according to the formula in the operation instructions. The content of malondialdehyde (MDA) in leaves was determined using the thiobarbituric acid (TBA) method [33].

4.7. Screening and Validation of LpPsbP-Interacting Proteins

The recombinant plasmid pGBKT7-LpPsbP was obtained by connecting the LpPsbP gene and pGBKT7 vector with BamH1 and Sal1 restriction site. pGBKT7-LpPsbP plasmid was transferred into Y2H with the screening of cDNA library. Additionally, the blue colonies were selected from SD-Trp-Leu-His plus X-α-gal medium. DNA was used as the template, while T7 and 3′-AD as upstream and downstream primers for PCR identification and the product of PCR was sequenced.
pGBKT7-LpPsbP and interacting protein-pGAD17 were co-transformed into Y2H Gold to examine their interactions. The colonies were cultured on SD-Trp-Leu-His plus X-α-gal medium. pGBKT7-53 plus pGADT7-T was used as positive control, and pGBKT7-lam plus pGADT7-T was used as negative control.
LpFoxO gene was cloned by PCR using L. pumilum cDNA as template (LpFoxO-F and LpFoxO-R, shown in Table 1). The LpFoxO and pGAD17 vectors were ligated through BamH1 and Xho1 restriction site. pGBKT7-LpPsbP and pGADT7-LpFoxO were co-transformed into Y2HGold. Then, the co-transformed bacterial liquid was placed in SD-Trp-Leu-His plus X-α-gal medium. Co-transformed strains of pGBKT7 and pGADT7, co-transformed strains of pGADT7 and pGBKT7-LpPsbP and co-transformed strains of pGBKT7 and pGADT7-LpFoxO were used as control groups.
The LpPsbP gene was constructed into the pCAMBIA1300-NLuc vector (primers: LpPsbP-SacI-F and LpPsbP-SalI-R, shown in Table 1) and the FoxO gene was cloned into pCAMBIA1300-CLuc vector (primers: FoxO-BamHI-F and FoxO-SalI-R, shown in Table 1). The constructed vector plasmids were transformed into Agrobacterium GV1301, and then injected into tobacco leaves. The leaves are exposed to the dark for two days before being injected with D-fluorescein potassium salt and then left in the dark for five minutes. The photos were taken using the Tanon-5200 Chemiluminescent Imaging System (Shanghai, China).

4.8. Cloning of the LpPsbP Promoter

The sequence of LpPsbP promoter was cloned using Takara chromosome walking kit (Tokyo, Japan) and analyzed using PlantCARE (http://bioinformatics.psb.ugent.be/webtools/plantcare/html/ (accessed on 7 January 2023)). Promoter cloning primers are LpPsbP SP1, LpPsbP SP2, and LpPsbP SP3 (shown in Table 1).

5. Conclusions

In this study, the LpPsbP gene was cloned from the bulbs of L. pumilum, and the following conclusions were drawn: 1. The PsbP protein is closely related to the PsbP of A. officinalis, F. agrestis and other plants. LpPsbP is highly conserved in domain DcrB, which belongs to the DcrB superfamily. 2. The maximal expression of the protein is obtained under the induction with 1 mM IPTG for 1 h. The pGEX-LpPsbP purified protein was obtained. The tolerance of bacteria solution including LpPsbP-GST fusion protein is higher than that only including the GST protein. 3. Transgenic tobacco plants are subjected to NaCl, Na2CO3, NaHCO3 and H2O2 stress. Compared with wild-type plants, seed germination, seedling growth, phenotypes of mature tobacco under stress treatment, leaf stomatal size, O2− and H2O2 staining in leaves were observed. The physiological parameters such as net photosynthetic rate (Pn), stomatal conductance (Gs), intercellular CO2 (Ci) and transpiration rate (Tr), as well as chlorophyll content, superoxide anion (O2−) content, H2O2 content and malondialdehyde (MDA) content of wild-type and transgenic lines under salt stress were measured. The presence of the LpPsbP gene can alleviate the reduction in gas exchange function in leaves induced by salt stress, reduce the accumulation of reactive oxygen species (ROS) and alleviate plant damage. 4. A total of 18 interacting fragments are screened out. The interaction between pGBKT7-LpPsbP and pGADT7-LpFoxO is verified by yeast two-hybrid. Moreover, the results of luciferase complementation assay further showed that LpPsbP and FoxO can interact with each other. 5. There are many cis-acting elements involved in the response of abiotic stresses, light and hormones in the promoter of LpPsbP, which ensures that this promoter contributes significantly to many biological metabolic pathways.
Considering the above, we eventually conclude that LpPsbP may improve the Saline-Alkali resistance of L. pumilum mainly through the molecular pathway of scavenging ROS: L. pumilum produces ROS at sites including chloroplasts after Saline-Alkali stress or oxidative stress (such as NaCl, NaHCO3, Na2CO3 and H2O2), and the sub-site of ROS production in chloroplasts is mainly at the center of the oxygen releasing complex. When L. pumilum encounters Saline-Alkali stress or oxidative stress, the expression level of LpPsbP will be increased. Then, by directly scavenging ROS, LpPsbP will protect the PS II of L. pumilum, reduce its damage and thus improve the Saline-Alkali resistance of the plant. Moreover, we speculate that Saline-Alkali stress or oxidative stress will also promote the production of jasmonic acid (JA), leading to another promotion pathway of LpPsbP expression. Additionally, another speculation we have is that because LpPsbP can interact with FoxO and both of them can scavenge ROS alone, this interacting complex will probably promote the scavenging of ROS, leading to a better protection and resistance (Figure 8).

Author Contributions

Funding acquisition, Y.J. and S.J. (Shumei Jin); Investigation, Y.J., Y.S., S.J. (Shangwei Ji), L.Z., Z.W., Y.X. and S.J. (Shumei Jin); Methodology, Y.J., Y.X. and S.J. (Shumei Jin); Project administration, Y.J. and Y.S.; Resources, Y.J., Y.S., S.J. (Shangwei Ji), L.Z., Z.W., Y.X. and S.J. (Shumei Jin); Software, Y.J., Y.S., Y.D., Y.X. and S.J. (Shumei Jin); Supervision, Y.J., Y.S. and S.J. (Shumei Jin); Writing—original draft, Y.J., L.Z., Y.D., Y.X. and S.J. (Shumei Jin); Writing—review and editing, Y.J. and S.J. (Shumei Jin). All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by Undergraduate Training Programs for Innovations by NEFU, grant number 202210225106 and Undergraduate Training Programs for Innovations by NEFU, grant number S202210225103.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Acknowledgments

Thanks for the support of Aulin College and College of Life Sciences in Northeast Forestry University.

Conflicts of Interest

The authors declare no conflict of interest.

Abbreviations

ROSReactive oxygen species
JAJasmonic acid
PS IIPhotosystem II
PsbPPS II subunit P
IPTGIsopropyl-beta-D-thiogalactopyranoside
GSTGlutathione S-transferase
ODOptical density
NBTNitro-blue tetrazolium
DABDiaminobenzidine
MDAMalondialdehyde
TBAThiobarbituric acid

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Figure 1. Bioinformatics analysis of LpPsbP. (A): Conservative domain analysis of LpPsbP using NCBI. Result shows that there is a highly conserved DcrB domain in LpPsbP. (B): Amino acid sequence alignment of LpPsbP with other homologous proteins of different plant species. The amino acid sequence of this transcript was similar to that alignment of the PsbP amino acid sequence from other species: Fritillaria agrestis, FaPsbP, O49080.1, 89.77%; Nyssa sinensis, NsPsbP, KAA8524123.1, 75.46%; Ananas comosus, AcPsbP, XP_020096384.1, 75.09%; Cinnamomum micranthum F. Kanehirae, CmPsbP, RWR81876.1, 73.98%; Nelumbo nucifera, NnPsbP, XP_010247826.1, 73.64%; Tetracentron sinense, TsPsbP, KAF8408713.1, 75.46%; Elaeis guineensis, EgPsbP, XP_010906959.1, 75.00%; Musa balbisiana, MbPsbP, THU61210.1, 77.53%; Asparagus officinalis, AoPsbP, XP_020256197.1, 73.23%; Ipomoea triloba, LtPsbP, XP_031126809.1, 75.75%; Pistacia vera, PvPsbP, XP_031257256.1, 73.23%; Musa acuminata, MaPsbP, XP_009407699.1, 76.78%. (C): Phylogenetic tree analysis of LpPsbP and its homologous proteins. The scale bar represents that there is a 5% difference in this given length. The related species are shown in (B).
Figure 1. Bioinformatics analysis of LpPsbP. (A): Conservative domain analysis of LpPsbP using NCBI. Result shows that there is a highly conserved DcrB domain in LpPsbP. (B): Amino acid sequence alignment of LpPsbP with other homologous proteins of different plant species. The amino acid sequence of this transcript was similar to that alignment of the PsbP amino acid sequence from other species: Fritillaria agrestis, FaPsbP, O49080.1, 89.77%; Nyssa sinensis, NsPsbP, KAA8524123.1, 75.46%; Ananas comosus, AcPsbP, XP_020096384.1, 75.09%; Cinnamomum micranthum F. Kanehirae, CmPsbP, RWR81876.1, 73.98%; Nelumbo nucifera, NnPsbP, XP_010247826.1, 73.64%; Tetracentron sinense, TsPsbP, KAF8408713.1, 75.46%; Elaeis guineensis, EgPsbP, XP_010906959.1, 75.00%; Musa balbisiana, MbPsbP, THU61210.1, 77.53%; Asparagus officinalis, AoPsbP, XP_020256197.1, 73.23%; Ipomoea triloba, LtPsbP, XP_031126809.1, 75.75%; Pistacia vera, PvPsbP, XP_031257256.1, 73.23%; Musa acuminata, MaPsbP, XP_009407699.1, 76.78%. (C): Phylogenetic tree analysis of LpPsbP and its homologous proteins. The scale bar represents that there is a 5% difference in this given length. The related species are shown in (B).
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Figure 2. Expression characteristics of LpPsbP gene in the leaves of L. pumilum after stress treatment. The RNA in the leaves was extracted and then reverse-transcribed into cDNA and the levels of relative expression are gained through qRT-PCR. “*” indicates significant difference, Student’s t-test (P<0.05, ±SEM). “**” indicates extremely significant difference. (A): Expression of LpPsbP gene at 11 mM H2O2 treatment at different times. (B): Expression levels of LpPsbP gene at 200 mM NaCl treatment at different times. (C): Expression levels of LpPsbP gene at 20 mM NaHCO3 treatment at different times. (D): Expression levels of LpPsbP gene at 20 mM Na2CO3 treatment at different times.
Figure 2. Expression characteristics of LpPsbP gene in the leaves of L. pumilum after stress treatment. The RNA in the leaves was extracted and then reverse-transcribed into cDNA and the levels of relative expression are gained through qRT-PCR. “*” indicates significant difference, Student’s t-test (P<0.05, ±SEM). “**” indicates extremely significant difference. (A): Expression of LpPsbP gene at 11 mM H2O2 treatment at different times. (B): Expression levels of LpPsbP gene at 200 mM NaCl treatment at different times. (C): Expression levels of LpPsbP gene at 20 mM NaHCO3 treatment at different times. (D): Expression levels of LpPsbP gene at 20 mM Na2CO3 treatment at different times.
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Figure 3. Protein induction experiment. (A): pGEX-LpPsbP induction at different IPTG concentrations (M: protein marker; 1, 2, 3, 4, 5: the expression of LpPsbP protein was induced by 0, 0.5, 1, 1.5, 2 mM IPTG); (B): pGEX-LpPsbP induction at different times (M: protein marker; 1, 2, 3, 4, 5, 6, 7: the expression of LpPsbP protein was induced for 0, 0.5, 1, 2, 3, 4, 5 h, respectively). (C): pGEX-LpPsbP protein purification using purification column with GST purification resin. M: protein marker; 1: induced without IPTG; 2: induced with 1 mM IPTG for 5 h; 3: pGEX-LpPsbP renatured protein; 4: pGEX-LpPsbP purified protein. (D): Growth curves of Escherichia coli under different Saline-Alkali stresses. a: The OD600 values of pGEX and LpPsbP induced for 5 h under untreated conditions (CK). b: The OD600 value of bacterial solution after 5 h induction by pGEX and LpPsbP under the treatment of 0.2 mM NaHCO3. c: The OD600 value of bacterial liquid after 5 h induction by pGEX and LpPsbP under the treatment of 0.1 mM Na2CO3. d: The OD600 value of bacterial solution after 5 h induction by pGEX and LpPsbP under the treatment of 0.8 mM NaCl.
Figure 3. Protein induction experiment. (A): pGEX-LpPsbP induction at different IPTG concentrations (M: protein marker; 1, 2, 3, 4, 5: the expression of LpPsbP protein was induced by 0, 0.5, 1, 1.5, 2 mM IPTG); (B): pGEX-LpPsbP induction at different times (M: protein marker; 1, 2, 3, 4, 5, 6, 7: the expression of LpPsbP protein was induced for 0, 0.5, 1, 2, 3, 4, 5 h, respectively). (C): pGEX-LpPsbP protein purification using purification column with GST purification resin. M: protein marker; 1: induced without IPTG; 2: induced with 1 mM IPTG for 5 h; 3: pGEX-LpPsbP renatured protein; 4: pGEX-LpPsbP purified protein. (D): Growth curves of Escherichia coli under different Saline-Alkali stresses. a: The OD600 values of pGEX and LpPsbP induced for 5 h under untreated conditions (CK). b: The OD600 value of bacterial solution after 5 h induction by pGEX and LpPsbP under the treatment of 0.2 mM NaHCO3. c: The OD600 value of bacterial liquid after 5 h induction by pGEX and LpPsbP under the treatment of 0.1 mM Na2CO3. d: The OD600 value of bacterial solution after 5 h induction by pGEX and LpPsbP under the treatment of 0.8 mM NaCl.
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Figure 4. Phenotypic analysis of LpPsbP overexpressed tobaccos. (A): qPCR identification of transgenic tobacco overexpressing LpPsbP gene. cDNA was obtained from the leaves of wild-type and transgenic tobacco plants by reverse-transcribing from RNA. The expression levels were detected by qPCR. WT: wild line; #1-#7: LpPsbP transgenic line; “**” indicates extremely significant difference. (B): Germination phenotypic analysis of wild-type tobaccos and LpPsbP transgenic tobaccos under salt stress. Tobacco seeds from the T3 generation, both wild-type and LpPsbP transgenic, were taken and cultured for 7 days in 1/2 MS medium and 1/2 MS media supplemented with 4 mM H2O2, 125 mM NaCl, 8 mM NaHCO3 and 8 mM Na2CO3, respectively. (C): Seeding growth of LpPsbP transgenic plants under salt stress. Wild-type tobacco seeds and T3 generation LpPsbP transgenic plants were sown separately in control group and groups in 1/2 MS medium supplemented with 3 mM H2O2, 125 mM NaCl, 4 mM NaHCO3 and 4 mM Na2CO3. The results were recorded after 14 days. (D): Phenotypic analysis of LpPsbP transgenic plants under salt stress. Plants were irrigated with 20 mL of 1 M H2O2, 1 M NaCl, 0.5 M NaHCO3 and 0.5 M Na2CO3 solutions for 7 days. (E): Stomatal size of wildtype and LpPsbP transgenic tobacco under different salt stresses. It can be seen from this figure that after treatment with 4 mM H2O2, 125 mM NaCl, 8 mM NaHCO3 and 8 mM Na2CO3, stomatas in WT plants obviously closed, while LpPsbP transgenic plants only displayed slight stomata closure. (F): NBT and DAB staining of various LpPsbP-related genetic materials under salt stress. In situ NBT and DAB staining methods were used to detect the accumulation of O2− and H2O2 in seedling leaves, which reflected the ability of LpPsbP to reduce ROS content in plants. The deeper the blue color is, the more O2− accumulated. The darker the brown color is, the more H2O2 is accumulated.
Figure 4. Phenotypic analysis of LpPsbP overexpressed tobaccos. (A): qPCR identification of transgenic tobacco overexpressing LpPsbP gene. cDNA was obtained from the leaves of wild-type and transgenic tobacco plants by reverse-transcribing from RNA. The expression levels were detected by qPCR. WT: wild line; #1-#7: LpPsbP transgenic line; “**” indicates extremely significant difference. (B): Germination phenotypic analysis of wild-type tobaccos and LpPsbP transgenic tobaccos under salt stress. Tobacco seeds from the T3 generation, both wild-type and LpPsbP transgenic, were taken and cultured for 7 days in 1/2 MS medium and 1/2 MS media supplemented with 4 mM H2O2, 125 mM NaCl, 8 mM NaHCO3 and 8 mM Na2CO3, respectively. (C): Seeding growth of LpPsbP transgenic plants under salt stress. Wild-type tobacco seeds and T3 generation LpPsbP transgenic plants were sown separately in control group and groups in 1/2 MS medium supplemented with 3 mM H2O2, 125 mM NaCl, 4 mM NaHCO3 and 4 mM Na2CO3. The results were recorded after 14 days. (D): Phenotypic analysis of LpPsbP transgenic plants under salt stress. Plants were irrigated with 20 mL of 1 M H2O2, 1 M NaCl, 0.5 M NaHCO3 and 0.5 M Na2CO3 solutions for 7 days. (E): Stomatal size of wildtype and LpPsbP transgenic tobacco under different salt stresses. It can be seen from this figure that after treatment with 4 mM H2O2, 125 mM NaCl, 8 mM NaHCO3 and 8 mM Na2CO3, stomatas in WT plants obviously closed, while LpPsbP transgenic plants only displayed slight stomata closure. (F): NBT and DAB staining of various LpPsbP-related genetic materials under salt stress. In situ NBT and DAB staining methods were used to detect the accumulation of O2− and H2O2 in seedling leaves, which reflected the ability of LpPsbP to reduce ROS content in plants. The deeper the blue color is, the more O2− accumulated. The darker the brown color is, the more H2O2 is accumulated.
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Figure 5. Analysis of physiological indices of tobacco under Saline-Alkali stress. “*” indicates significant difference, Student’s t-test (P<0.05, ±SEM). “**” indicates extremely significant difference. (A): Gas exchange parameters of LpPsbP tobacco under salt stress. LI-6400 photosynthometer was used to measure the following indices. (a): Net photosynthetic rate (Pn), (b): Stomatal conductance (Gs), (c): Intercellular CO2 (Ci), (d): Transpiration rate (Tr) of wild-type and LpPsbP transgenic tobacco under different salt treatments (1 M H2O2, 1 M NaCl, 0.5 M NaHCO3 and 0.5 M Na2CO3). (B): Determination of chlorophyll content in LpPsbP tobacco under salt stress. SPAD chlorophyll meter was used to measure the chlorophyll contents of tobacco under different salt stresses (125 mM NaCl, 8 mM Na2CO3, 8 mM NaHCO3 and 4 mM H2O2). (C): Determination of O2− content in LpPsbP tobacco under salt stress (125 mM NaCl, 8 mM Na2CO3, 8 mM NaHCO3 and 4 mM H2O2). Oxidative damage and O2− accumulation in plants are both consequences of salt stress. By evaluating the O2− content in transgenic plants, it was possible to evaluate the capacity of LpPsbP to reduce ROS content in tobacco. (D): Determination of H2O2 content in LpPsbP tobacco under salt stress. An H2O2 kit (Grace, Suzhou, China) was used to measure the H2O2 content of wild-type and transgene plants under controlled and salt stress (125 mM NaCl, 8 mM Na2CO3, 8 mM NaHCO3 and 4 mM H2O2). (E): Determination of MDA content in LpPsbP tobacco under salt stress. The levels of malondialdehyde (MDA) produced by several tobacco lines when exposed to salt stress (125 mM NaCl, 8 mM Na2CO3, 8 mM NaHCO3 and 4 mM H2O2) were examined to evaluate the relationship between excessive ROS accumulation and cell damage.
Figure 5. Analysis of physiological indices of tobacco under Saline-Alkali stress. “*” indicates significant difference, Student’s t-test (P<0.05, ±SEM). “**” indicates extremely significant difference. (A): Gas exchange parameters of LpPsbP tobacco under salt stress. LI-6400 photosynthometer was used to measure the following indices. (a): Net photosynthetic rate (Pn), (b): Stomatal conductance (Gs), (c): Intercellular CO2 (Ci), (d): Transpiration rate (Tr) of wild-type and LpPsbP transgenic tobacco under different salt treatments (1 M H2O2, 1 M NaCl, 0.5 M NaHCO3 and 0.5 M Na2CO3). (B): Determination of chlorophyll content in LpPsbP tobacco under salt stress. SPAD chlorophyll meter was used to measure the chlorophyll contents of tobacco under different salt stresses (125 mM NaCl, 8 mM Na2CO3, 8 mM NaHCO3 and 4 mM H2O2). (C): Determination of O2− content in LpPsbP tobacco under salt stress (125 mM NaCl, 8 mM Na2CO3, 8 mM NaHCO3 and 4 mM H2O2). Oxidative damage and O2− accumulation in plants are both consequences of salt stress. By evaluating the O2− content in transgenic plants, it was possible to evaluate the capacity of LpPsbP to reduce ROS content in tobacco. (D): Determination of H2O2 content in LpPsbP tobacco under salt stress. An H2O2 kit (Grace, Suzhou, China) was used to measure the H2O2 content of wild-type and transgene plants under controlled and salt stress (125 mM NaCl, 8 mM Na2CO3, 8 mM NaHCO3 and 4 mM H2O2). (E): Determination of MDA content in LpPsbP tobacco under salt stress. The levels of malondialdehyde (MDA) produced by several tobacco lines when exposed to salt stress (125 mM NaCl, 8 mM Na2CO3, 8 mM NaHCO3 and 4 mM H2O2) were examined to evaluate the relationship between excessive ROS accumulation and cell damage.
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Figure 6. Validation of LpPsbP interacting proteins. (A): cDNA library screening of pGBKT7-LpPsbP. Y2H was cotransformed successfully with 5 plasmids related to salt stress and pGBKT7-LpPsbP. When the yeast was grown on SD-Trp-Leu-His plus X-gal media at 30 °C, the transformed yeasts with plasmids 2 and 5 grew as colonies and became blue, while the yeasts with plasmids 1, 3 and 4 did not. (B): pGBKT7-LpPsbP and pGADT7-LpFoxO co-conversion validation. On SD-Trp-Leu-His plus X-gal media, the strains cotransformed with pGBKT7-LpPsbP and pGADT7-LpFoxO were able to establish blue colonies, whereas the control group was unable to do that. (C): Luciferase complementation assay. The first quadrant: both NLuc and CLuc were unloaded, the second quadrant: NLuc was unloaded, the third quadrant: CLuc was unloaded and the fourth quadrant: pCAMBIA1300-NLuc-PsbP and pCAMBIA1300-CLuc-FoxO.
Figure 6. Validation of LpPsbP interacting proteins. (A): cDNA library screening of pGBKT7-LpPsbP. Y2H was cotransformed successfully with 5 plasmids related to salt stress and pGBKT7-LpPsbP. When the yeast was grown on SD-Trp-Leu-His plus X-gal media at 30 °C, the transformed yeasts with plasmids 2 and 5 grew as colonies and became blue, while the yeasts with plasmids 1, 3 and 4 did not. (B): pGBKT7-LpPsbP and pGADT7-LpFoxO co-conversion validation. On SD-Trp-Leu-His plus X-gal media, the strains cotransformed with pGBKT7-LpPsbP and pGADT7-LpFoxO were able to establish blue colonies, whereas the control group was unable to do that. (C): Luciferase complementation assay. The first quadrant: both NLuc and CLuc were unloaded, the second quadrant: NLuc was unloaded, the third quadrant: CLuc was unloaded and the fourth quadrant: pCAMBIA1300-NLuc-PsbP and pCAMBIA1300-CLuc-FoxO.
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Figure 7. Visual analysis of the LpPsbP promoter. The acquired promoter sequences underwent visual analysis using the TBtools software to present some cis-acting elements such as GT1-motif and P-box. Sequence analysis revealed that this sequence comprised numerous responsive and regulatory regions.
Figure 7. Visual analysis of the LpPsbP promoter. The acquired promoter sequences underwent visual analysis using the TBtools software to present some cis-acting elements such as GT1-motif and P-box. Sequence analysis revealed that this sequence comprised numerous responsive and regulatory regions.
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Figure 8. Concluded mechanism of how LpPsbP improves Saline-Alkali resistance in L. pumilum. After Saline-Alkaline stress and oxidative stress (caused by NaCl, NaHCO3, Na2CO3 and H2O2), L. pumilum creates ROS at a variety of locations, including chloroplasts. The sub-site of ROS generation in chloroplasts is mostly at the center of the oxygen-releasing complex. When encountering these stresses, the expression of LpPsbP is promoted. Then, LpPsbP will scavenge ROS, which means it will finally safeguard the PS II of L. pumilum, lessen damage to it, and therefore increase the ability of plants to tolerate stress. Moreover, we have made two additional speculations: 1. Following stress, L. pumilum generates more JA, which directly promotes the expression of the PsbP gene. 2. First comes with the fact that the FoxO gene will express itself more when ROS are created, which means that L. pumilum encounters these two kinds of stress. Then, because these two ROS scavenging proteins (LpPsbP and FoxO) can interact with each other, it will promote the scavenging of ROS, leading to a better protection and resistance. This figure is made using BioRender website (https://biorender.com/), dashed lines in this figure means these are speculations instead of definitive knowledge.
Figure 8. Concluded mechanism of how LpPsbP improves Saline-Alkali resistance in L. pumilum. After Saline-Alkaline stress and oxidative stress (caused by NaCl, NaHCO3, Na2CO3 and H2O2), L. pumilum creates ROS at a variety of locations, including chloroplasts. The sub-site of ROS generation in chloroplasts is mostly at the center of the oxygen-releasing complex. When encountering these stresses, the expression of LpPsbP is promoted. Then, LpPsbP will scavenge ROS, which means it will finally safeguard the PS II of L. pumilum, lessen damage to it, and therefore increase the ability of plants to tolerate stress. Moreover, we have made two additional speculations: 1. Following stress, L. pumilum generates more JA, which directly promotes the expression of the PsbP gene. 2. First comes with the fact that the FoxO gene will express itself more when ROS are created, which means that L. pumilum encounters these two kinds of stress. Then, because these two ROS scavenging proteins (LpPsbP and FoxO) can interact with each other, it will promote the scavenging of ROS, leading to a better protection and resistance. This figure is made using BioRender website (https://biorender.com/), dashed lines in this figure means these are speculations instead of definitive knowledge.
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Table
Table 1. Primers used in this study.
Table 1. Primers used in this study.
Primer NamePrimer Sequence
LpPsbP-FATGGCCTCATCTGCATGC
LpPsbP-RTCATGCAACATTGAAGGA
LpPsbP qPCR-FCACCAACCATCAAGCCCTCT
LpPsbP qPCR-RCCTGACCTGGGAACTCAACC
LpPsbP-BamH1-FGGATCCATGGCCTCATCTGCAT
LpPsbP-Xho1-RCTCGAGTCATGCAACATTGAAGGA
LpPsbP-Sal1-RGTCGACTCATGCAACATTGAAGGA
LpFoxO-FATGTCGATTAGCCGGGCA
LpFoxO-RTCATCTCCCAGCACAGTT
pGEX-LpPsbP-BamH1-FGGATCCATGGCCTCATCTGCAT
LpPsbP SP1ATGGCAGCAGAGCCAATGAGGA
LpPsbP SP2TTCTGAGCCCGACAGATGAGCT
LpPsbP SP3ACATGGTGGAGGAGGAAGCATG
T7TAATACGACTCACTATAGGGC
3′-ADAGATGGTCACGATGCACAG
LpPsbP-SacI-FGAGCTCATGGCATCGACAGC
LpPsbP-SalI-RGTCGACAGCAACATTGAAGG
FoxO-BamHI-FGGATCCATGTCGATTAGCCG
FoxO-SalI-RGTCGACTCATCTCCCAGCAC
Table
Table 2. The proteins coded by 5 sequences related to Saline-Alkali resistance.
Table 2. The proteins coded by 5 sequences related to Saline-Alkali resistance.
Protein NameFunctionsReference
Winged-helix DNA-binding transcription factor family proteinModulate DNA damage response through the regulation of SOG1 and ATR transcription level.[17]
Pollen Ole e 1 allergen and extensin family proteinResponse to salt stress by maintain the steady state of endoplasmic reticulum.[18]
Phosphatidyl glycerol phosphate synthase 2Stabilizing photosynthetic membrane by producing phosphatidyl glycerol phosphate in order to maintain the stability of plant under Saline-Alkali stress.
[19]
Putative lysine decarboxylase family proteinPlay an important role in direct activation pathway of cytokinesis.[20]
ARF-GAP domain 13Release dormancy of plant stem by affecting the transport of vesicle.[21]
Table
Table 3. Analysis of cis-acting elements in the promoter sequence of LpPsbP gene.
Table 3. Analysis of cis-acting elements in the promoter sequence of LpPsbP gene.
Cis-ElementMotif (5′-3′)FunctionFrequency
ABREACGTGCis-acting element
involved in the abscisic
acid responsiveness.		1
AREAAACCACis-acting regulatory
element essential for the anaerobic induction.		2
CGTCA-motifCGTCACis-acting regulatory element involved in the MeJA-responsiveness.1
GATA-motifGATAGGGPart of a light responsive element.2
GC-motifCCCCCGEnhancer-like element involved in anoxic specific inducibility.2
GT1-motifGGTTAATLight responsive element.1
P-boxCCTTTTGGibberellin-responsive
element.		1
TGACG-motifTGACGCis-acting regulatory element involved in the MeJA-responsiveness.1
CircadianCAAAGATATCCis-acting regulatory element involved in circadian control.1
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MDPI and ACS Style

Jing, Y.; Song, Y.; Ji, S.; Zhang, L.; Wang, Z.; Dong, Y.; Xu, Y.; Jin, S. PS II Subunit P in Lilium pumilum (LpPsbP) Confers Saline-Alkali Resistance to the Plant by Scavenging ROS. Int. J. Mol. Sci. 2023, 24, 3311. https://doi.org/10.3390/ijms24043311

AMA Style

Jing Y, Song Y, Ji S, Zhang L, Wang Z, Dong Y, Xu Y, Jin S. PS II Subunit P in Lilium pumilum (LpPsbP) Confers Saline-Alkali Resistance to the Plant by Scavenging ROS. International Journal of Molecular Sciences. 2023; 24(4):3311. https://doi.org/10.3390/ijms24043311

Chicago/Turabian Style

Jing, Yibo, Yu Song, Shangwei Ji, Ling Zhang, Zongying Wang, Yi Dong, Yang Xu, and Shumei Jin. 2023. "PS II Subunit P in Lilium pumilum (LpPsbP) Confers Saline-Alkali Resistance to the Plant by Scavenging ROS" International Journal of Molecular Sciences 24, no. 4: 3311. https://doi.org/10.3390/ijms24043311

APA Style

Jing, Y., Song, Y., Ji, S., Zhang, L., Wang, Z., Dong, Y., Xu, Y., & Jin, S. (2023). PS II Subunit P in Lilium pumilum (LpPsbP) Confers Saline-Alkali Resistance to the Plant by Scavenging ROS. International Journal of Molecular Sciences, 24(4), 3311. https://doi.org/10.3390/ijms24043311

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