Next Article in Journal
Involvement of Mast-Cell-Tryptase- and Protease-Activated Receptor 2—Mediated Signaling and Urothelial Barrier Dysfunction with Reduced Uroplakin II Expression in Bladder Hyperactivity Induced by Chronic Bladder Ischemia in the Rat
Next Article in Special Issue
The Effect of Ketamine on Endoplasmic Reticulum Stress in Rats with Neuropathic Pain
Previous Article in Journal
Specific Mutations near the Amyloid Precursor Protein Cleavage Site Increase γ-Secretase Sensitivity and Modulate Amyloid-β Production
Previous Article in Special Issue
Endoplasmic Reticulum Stress Causing Apoptosis in a Mouse Model of an Ischemic Spinal Cord Injury
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 24
Issue 4
10.3390/ijms24043987
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Oxidative-Stress-Mediated ER Stress Is Involved in Regulating Manoalide-Induced Antiproliferation in Oral Cancer Cells

by
Sheng-Yao Peng
1,
Jen-Yang Tang
2,3,
Ting-Hsun Lan
4,5,
Jun-Ping Shiau
6,
Kuan-Liang Chen
7,
Jiiang-Huei Jeng
4,5,8,
Ching-Yu Yen
7,9,* and
Hsueh-Wei Chang
1,10,11,*
1
Department of Biomedical Science and Environmental Biology, PhD Program in Life Sciences, College of Life Sciences, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
2
School of Post-Baccalaureate Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
3
Department of Radiation Oncology, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan
4
Division of Prosthodontics, Department of Dentistry, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan
5
School of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
6
Division of Breast Oncology and Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
7
Department of Oral and Maxillofacial Surgery, Chi-Mei Medical Center, Tainan 71004, Taiwan
8
Department of Dentistry, National Taiwan University Hospital, Taipei 100225, Taiwan
9
School of Dentistry, Taipei Medical University, Taipei 11031, Taiwan
10
Center for Cancer Research, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
11
Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan
 
add Show full affiliation list
 
remove Hide full affiliation list
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2023, 24(4), 3987; https://doi.org/10.3390/ijms24043987
Submission received: 10 January 2023 / Revised: 6 February 2023 / Accepted: 15 February 2023 / Published: 16 February 2023
(This article belongs to the Special Issue Endoplasmic Reticulum Stress-Related Biomedical Implications)
Browse Figures
Versions Notes

Abstract

:
Manoalide provides preferential antiproliferation of oral cancer but is non-cytotoxic to normal cells by modulating reactive oxygen species (ROS) and apoptosis. Although ROS interplays with endoplasmic reticulum (ER) stress and apoptosis, the influence of ER stress on manoalide-triggered apoptosis has not been reported. The role of ER stress in manoalide-induced preferential antiproliferation and apoptosis was assessed in this study. Manoalide induces a higher ER expansion and aggresome accumulation of oral cancer than normal cells. Generally, manoalide differentially influences higher mRNA and protein expressions of ER-stress-associated genes (PERK, IRE1α, ATF6, and BIP) in oral cancer cells than in normal cells. Subsequently, the contribution of ER stress on manoalide-treated oral cancer cells was further examined. ER stress inducer, thapsigargin, enhances the manoalide-induced antiproliferation, caspase 3/7 activation, and autophagy of oral cancer cells rather than normal cells. Moreover, N-acetylcysteine, an ROS inhibitor, reverses the responses of ER stress, aggresome formation, and the antiproliferation of oral cancer cells. Consequently, the preferential ER stress of manoalide-treated oral cancer cells is crucial for its antiproliferative effect.

1. Introduction

Oral cancer is a severe type of cancer that is of increasing global concern [1]. The main factors correlated with mouth cancer include dangerous exposure to substance use, such as higher drinking, smoking, and betel nut chewing behaviors [2]. Additionally, viruses, fungus infections, nutritional deficiencies, radiation exposure, poor oral hygiene, and other chronic physical and chemical stimulations may be co-factors. In addition, gender, age, ethnicity, geographical culture, and lifestyle habits also affect the incidence of oral cancer [3]. There is also increasing research investment in the correlation between genes and environmental factors and the occurrence and deterioration of oral cancer [4]. Clinically, chemoradiotherapy and radiotherapy are used for oral cancer therapy, aside from surgery, but they occasionally generate adverse effects. Anti-cancer drugs with non-cytotoxicity that improve oral cancer therapy warrant continuous development.
Marine natural products are a diverse resource for the discovery of anti-cancer drugs [5,6,7,8]. Marine sponges are rich in many bioactive compounds [9,10,11], particularly for anti-cancer treatment. Manoalide is a sesterterpenoid, first isolated from a marine sponge Luffariella variabilis in 1980, and exhibits antibiotic [12], analgesic, and anti-inflammatory effects [13]. In 1993 and 2018, the repurposed application of manoalide for anti-cancer impacts was reported in murine lymphoma LI210 [14], cholangiocarcinoma, leukemia, and cervical cancer cells [15]. However, these studies [14,15] focused on isolating chemical compounds in the marine sponge, and they only provided IC50 values for anti-cancer evidence without investigating the detailed mechanisms.
The endoplasmic reticulum (ER) is a dynamic organelle that regulates diverse cellular functions, such as protein synthesis/folding, lipid synthesis/metabolism, and calcium storage/release, in response to cell stress and drug treatments [16,17]. ER stress is a condition in which many misfolding proteins are accumulated, which exceed the tolerance of protein refolding of ER. In turn, ER stress initiates the unfolded protein response (UPR). Heat-shock protein family A member 5 (HSPA5; GRP-78; BIP) is a master protein for ER stress regulation that connects to three primary UPR proteins, including protein kinase RNA-like ER kinase (EIF2AK3; PERK), inositol-requiring enzyme 1 alpha (ERN1; IRE1α), and activating transcription factor 6 (ATF6) [18]. Cellular stress causes ER dysfunction, such as protein immaturation and misfolding, which is a characteristic of the UPR [16]. Dramatic ER stress response generally triggers apoptosis [19,20] and autophagy [21,22] of cancer cells.
ER stress interplays with reactive oxygen species (ROS) in regulating redox signaling [23]. Disturbance of ROS homeostasis causes cellular and mitochondrial ROS accumulation, triggering ER stress [24,25]. Drugs with modulating functions for ROS and ER stress show potential effects in promoting cancer cell death [26,27]. For example, pendulone, a Millettia dielsiana-derived isoflavone, promotes apoptosis of lung cancer cells by upregulating ROS-dependent ER stress [28]. Several drugs showing modulation of ER stress have preferential antiproliferative effects on cancer cells without exhibiting cytotoxicity to normal cells [26]. Our previous reports demonstrate the detailed mechanism of manoalide in preferentially inhibiting the proliferation of oral cancer cells (which is not cytotoxic to normal cells), while upregulating cellular and mitochondrial ROS, DNA damage, and apoptosis [29,30].
Because ROS can induce ER stress as mentioned above, the ER stress function and the downstream responses of oral cancer cells treated with manoalide warrants a detailed assessment. This study aims to assess ER stress responses and their impact on apoptosis and autophagy of oral cancer cells under manoalide treatment. The modulating effects of oxidative stress induced by manoalide on ER stress of oral cancer cells are also investigated.

2. Results

2.1. ER Expansion Change by Manoalide: Oral Cancer vs. Normal Cells

The changes in ER expansion patterns at different concentrations and exposure times of manoalide-treated oral cancer cells were assessed by flow cytometry (Figure 1A,C). Manoalide increased the intensity of ER (+) (%), namely ER expansion, in oral cancer cells (CAL 27 and Ca9-22) (Figure 1B,D). In contrast, ER expansion did not appear in normal cells (SG) because their ER staining intensities were similar to that of the control after manoalide treatment. Consequently, manoalide preferentially triggers ER expansion in oral cancer cells compared to normal cells.
Moreover, the influence of oxidative stress triggered by manoalide in controlling ER expansion was evaluated by the pretreatment of N-acetylcysteine (NAC) (Figure 1C). Manoalide-induced ER expansion of oral cancer cells was downregulated by NAC (Figure 1D). Hence, the role of ROS in manoalide-triggered ER expansion in oral cancer cells was confirmed.

2.2. Aggresome Change by Manoalide: Oral Cancer vs. Normal Cells

When ER stress is triggered, misfolded or denatured proteins are accumulated to form aggresomes [31]. The changes in aggresome generation patterns at different concentrations and exposure times of manoalide treatment in oral cancer cells were assessed by flow cytometry (Figure 2A,C). Manoalide increased the intensity of aggresome (+) (%) in oral cancer cells (Figure 2B,D). Consequently, manoalide preferentially triggers aggresome formation in oral cancer cells compared to normal cells.
Moreover, the presence of NAC estimated the oxidative stress involvement in modulating aggresome generation (Figure 2C). The manoalide-triggered aggresome accumulation of oral cancer cells was downregulated by NAC (Figure 2D). Hence, the role of ROS in manoalide-triggered aggresome formation in oral cancer cells was confirmed.

2.3. Modulation of ER Stress Signaling by Manoalide: Oral Cancer vs. Normal Cells

The mRNA expressions for ER stress signaling [32], including BIP, PERK, IRE1α, and ATF6, were assessed for manoalide treatment for 24 h in oral cancer cells and normal cells. The relative mRNA expression of these ER-stress-associated genes (BIP, PERK, IRE1α, and ATF6) in oral cancer cells was generally higher than in the control, while it showed no change in normal cells (Figure 3A). BIP, IRE1α, and ATF6 genes were upregulated in oral cancer (CAL 27) cells at a high dose (10 μM). IRE1α and ATF6 genes were overexpressed in oral cancer (Ca9-22) cells at 5 and 10 μM of manoalide. In contrast, the mRNA expressions of these ER-stress-associated genes of normal cells (SG) did not change.
Similarly, protein expressions for ER stress signaling were upregulated by manoalide in different oral cancer cells (Figure 3B). In CAL 27 cells, BIP, PERK, and IRE1α were moderately upregulated, and ATF6 was slightly regulated. In Ca9-22 cells, only BIP and IRE1α were moderately upregulated. In comparison, the protein expressions of these ER stress genes of normal cells (SG) were similar to the control and were lower than oral cancer cells. Notably, the moderately upregulated ER stress genes of manoalide treatment in oral cancer cells were downregulated by NAC. Consequently, manoalide preferentially triggers ER stress signaling in oral cancer cells compared to normal cells. Moreover, the role of ROS in manoalide-triggered ER stress signaling in oral cancer cells was confirmed.

2.4. Antiproliferation by Manoalide and ER Stress Inducer: Oral Cancer vs. Normal Cells

To investigate the proliferation impact of manoalide-triggered ER stress, cells were co-treated with the ER stress inducer thapsigargin (TG), followed by manoalide treatment. Their modulating abilities on the proliferation of oral cancer cells and normal cells were examined by ATP analyses because ATP content is a common cell viability indicator [33,34]. Under ATP-content-based cell viability, manoalide showed a preferential antiproliferative effect on oral cancer cells, while it showed minor changes in normal cells (Figure 4A). TG alone dramatically decreased the cell proliferation of oral cancer cells and slightly reduced it in normal cells. When the combined treatment of TG/manoalide was performed, the antiproliferative effect was enhanced in oral cancer cells. Furthermore, the combined treatment of manoalide/TG remains similarly viable to TG alone in normal cells (SG).
To explore the role of ROS, NAC was pretreated prior to the post-treatments of manoalide and/or TG in oral cancer cells and normal cells. Both ATP (Figure 4B) and MTS (Figure 4C) assays demonstrated the upregulated viabilities of manoalide and/or TG treatments in oral cancer cells upon NAC.

2.5. Modulation of Apoptosis by Manoalide and ER Stress Inducer: Oral Cancer vs. Normal Cells

The impact of ER stress on manoalide-induced apoptosis was assessed by caspase 3/7 analyses. TG activated more caspase 3/7 activity in oral cancer cells than in the control. Moreover, TG/manoalide activated more caspase 3/7 activity than manoalide alone in oral cancer cells (CAL 27 and Ca9-22), particularly at a high dose (10 μM) (Figure 5). In contrast, the caspase 3/7 activity of manoalide treatment remained unchanged in normal cells (SG) with or without TG.

2.6. Modulation of Autophagy by Manoalide and ER Stress Inducer: Oral Cancer vs. Normal Cells

The impact of ER stress on manoalide-induced autophagy was assessed by DAPGreen (DAP) [35] and DALGreen (DAL) [36] analyses. Manoalide induced more DAP (+) and DAL (+) levels in oral cancer cells than in the control (Figure 6A,B). Moreover, TG/manoalide induced more DAP (+) and DAL (+) levels than manoalide alone in oral cancer cells (CAL 27 and Ca9-22) (Figure 6A,B). In contrast, the DAP (+) and DAL (+) levels of manoalide treatment remained unchanged in normal cells (SG) with or without TG.

3. Discussion

We previously found that manoalide provided preferential killing and the induction of oxidative stress, DNA damage, and apoptosis of oral cancer cells, while it showed minor changes to normal cells [29,30]. However, the interplay of ROS and apoptosis with ER stress [37] and the function of ER stress in the manoalide-caused antiproliferative effect of oral cancer were not investigated. The present study assessed the ER stress responses/signaling and apoptosis by modulating ROS or ER stress in the manoalide treatment of oral cancer cells and normal cells.
As detected by flow cytometry, manoalide at a high dose (10 μM) induced higher ER expansion and aggresome accumulation of oral cancer cells than normal cells (Figure 1 and Figure 2). These ER stress phenotypes were further examined by analyzing the mRNA and protein expression of ER stress signaling genes (Figure 3). CAL 27 and Ca9-22 cancer cells showed a higher mRNA expression of BIP, IRE1α, and ATF6 genes than the untreated control, particularly at 10 μM manoalide, but these were slightly changed in normal cells. In comparison, manoalide did not induce PERK mRNA expression in oral cancer cells.
Similarly, the protein expressions of BIP, PERK, IRE1α, and ATF6 genes of oral cancer cells and normal cells showed a similar tendency in their mRNA expressions. Notably, these ER-stress-associated proteins provided lower levels in normal cells than in oral cancer cells. This suggests that manoalide shows preferential induction of ER stress signaling in oral cancer, while it shows little changes in normal cells. However, the current study did not examine the impact of ER stress signaling on manoalide-triggered responses. It warrants a thoughtful assessment of knockdown BIP and/or IRE1α to see if the knockdown alters the outcomes of studies concerning viability, ER expansion, and apoptosis in the future.
Prolonged exposure to manoalide causes higher ER expansion and aggresome accumulation of oral cancer cells than normal cells, reversed by NAC. Similarly, some ER-stress-associated genes showing higher mRNA and protein expressions in oral cancer cells following manoalide treatment were reversed by NAC. This suggests that manoalide-triggered ER stress and its signaling are ROS-dependent. The role of ROS acting on apoptosis has been reported in our previous work [29,30], indicating that manoalide triggers apoptosis of oral cancer cells depending on ROS levels. Moreover, ER stress [21] and ROS [38] may trigger autophagy. This warrants a thorough investigation of the relationship between ROS, apoptosis, autophagy, and ER stress.
ER stress inhibits proliferation and triggers apoptosis of cancer cells [39,40,41,42]. TG, an ER stress inducer, suppresses the proliferation of breast cancer cells, and the combined treatment of TG with the engineered fusion protein (epidermal growth factor-proteolytic A subunit) enhances its antiproliferative effect [39]. TG also causes apoptosis of adrenocortical carcinoma [40] and prostate [41] cancer cells. Similarly, TG induces higher antiproliferation and apoptosis (caspase 3/7 activation) of manoalide-treated oral cancer cells than the control (Figure 4 and Figure 5). Like TG, manoalide triggers ER stress and apoptosis of oral cancer cells.
Furthermore, the impact of ER stress on manoalide-triggered antiproliferation, apoptosis, and autophagy was assessed in the present study. TG further enhances manoalide-induced antiproliferation, apoptosis (caspase 3/7 activation) (Figure 4 and Figure 5), and autophagy (Figure 6). In the example of hepatocytes, TG induces ER stress and antiproliferation, reversed by NAC [43], suggesting that TG triggers ROS induction to promote ER stress and antiproliferation. NAC can reverse the manoalide-induced antiproliferation [29] and ER stress responses (Figure 1, Figure 2 and Figure 3) of oral cancer cells. Similarly, NAC effects on the viability of TG/manoalide treatment of oral cancer cells were validated in the present study, demonstrating that TG/manoalide synergistically inhibited proliferation in an ROS-dependent manner (Figure 4B,C). Moreover, TG/manoalide synergistically triggered apoptosis (Figure 5), which may enhance antiproliferation. TG/manoalide also synergistically triggered autophagy (Figure 6); however, the role of autophagy in the antiproliferation of manoalide-treated oral cancer cells remains unclear. Consequently, manoalide exhibits the interplay of ER stress with ROS, apoptosis, and the preferential antiproliferation of oral cancer, but they are slightly changed in normal cells.
Besides apoptosis and autophagy, drug-induced ER stress may modulate and interact with several cellular functions, such as ferroptosis. For example, C2-ceramide triggers autophagy of liver cancer cells by upregulating oxidative stress and ER stress [44]. Notably, several anti-tumor drugs may exhibit the interplay between apoptosis and autophagy under ER stress [45,46,47]. Autophagy inhibitors promote apoptosis of prostate cancer cells triggered by overexpressing melanoma differentiation-associated gene 7 (mda-7) [48]. In contrast, downregulated apoptosis enhances autophagy after mda-7 overexpression. The relationship between ER-stress-modulated apoptosis and autophagy in manoalide-treated oral cancer cells may be explored by choosing their modulators. Moreover, ferroptosis triggers ER stress by interacting with apoptosis [49]. It warrants a thoughtful investigation for manoalide-induced other ER-stress-associated cell functions of oral cancer cells in the future.

4. Materials and Methods

4.1. Cell Culture and Chemicals

We chose the oral cancer cell lines (CAL 27 and Ca9-22) from the JCRB and ATCC for this study. As for normal oral cell line, we chose Smulow–Glickman (SG), a human normal gingival epithelial cell line that is well characterized [50,51] and commonly used to test drug safety for dental materials [52,53] and anti-oral-cancer drug development [54,55]. CAL 27/Ca9-22 and SG cells were cultured using mixtures of Dulbecco’s Modified Eagle Medium (DMEM) and F12 (Gibco, Grand Island, NY, USA) at ratios of 3:2 and 4:1 [29], respectively, with mixtures of 10% serum and common culture antibiotics.
Manoalide (CAYMAN CHEMICAL, Ann Arbor, MI, USA) and the ROS inhibitor NAC (Sigma-Aldrich, St. Louis, MO, USA) [56,57,58,59] were dissolved in dimethyl sulfoxide (DMSO) and 1X PBS. The DMSO concentration of all experiments (control and manoalide treatment) was the same for 0.1%. NAC at 10 mM was pretreated for 1 h before manoalide treatment. At 0.01 μM TG (Sigma-Aldrich), this ER stress inducer was co-treated by manoalide treatment.

4.2. ER Expansion

ER content was stable, but it increased (namely ER expansion) in response to ER stress. Using the Organelle-ID RGB® III Assay Kit (Enzo Life Sciences, Farmingdale, NY, USA) [60], the degree of ER expansion was monitored by flow cytometry. Briefly, cells were mixed with ER staining dye under incubation for 30 min at 4 °C. After washing with the medium twice, cells were stood for 30 min at 37 °C and analyzed using the Guava easyCyte flow cytometer (Luminex, Austin, TX, USA), plotting with FlowJo software (Becton-Dickinson, Franklin Lakes, NJ, USA).

4.3. Aggresome

Upon ER stress, aggresomes commonly increase. Aggresomes were stained using Proteostat® Aggresome Detection dye (Enzo Life Sciences) [61], and the degree of aggresome accumulation was determined by flow cytometry. Briefly, cells were prefixed with 4% paraformaldehyde for 30 min and permeabilized by 0.5% Triton X-100 for 30 min at 4 °C. Finally, cells were stained with aggresome staining dye (1:10,000) for 30 min, analyzed using a Guava easyCyte flow cytometer and plotted with FlowJo.

4.4. mRNA Expressions of ER-Stress-Associated Genes

RNA was extracted for cDNA conversion [62]. Quantitative RT-PCR (qRT-PCR) running with a touch-down PCR program was performed as described [62]. The mRNA expressions of four ER-stress-associated genes were analyzed [32], such as BIP, PERK, IRE1α, and ATF6. Their primer information is provided in Table 1. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was the control gene [63,64]. The fold activation of mRNA expressions was evaluated by 2-ΔΔCt calculations [65].

4.5. Western Blotting for ER-Stress-Associated Genes

The protein expressions of ER-stress-associated genes were detected by Western blotting by choosing their recognized antibodies, such as ATF6 (Abcam; 1:500), IRE1α, PERK, and BIP (Cell signaling, 1:1000), accompanied by the control antibody for β-actin (Sigma-Aldrich; 1:5000).

4.6. Cell Viability

Cell survival was readily detected by assessing intracellular ATP content (PerkinElmer Life Sciences, Boston, MA, USA) [66] and MTS assay (Promega Corporation, Madison, WI, USA) [67].

4.7. Caspase 3/7

Caspase 3/7 is the terminal caspase for apoptosis signaling. Caspase-Glo® 3/7 commercial product (Promega, Madison, WI, USA) was used to detect the degree of caspase 3/7 activation [66]. Briefly, caspase-3/7 tetrapeptide DEVD substrates were equally mixed with a reaction buffer for 30 min incubation at 37 °C in darkness. Finally, the luminescence generated by activated caspase 3/7 was detected by luminometers (Berthold Technologies GmbH & Co., Bad Wildbad, Germany). The caspase 3/7 activity for each treatment was calibrated by its cell viability [68].

4.8. Autophagy

According to user instructions, autophagy-detecting dyes (Dojindo, Kumamoto, Japan), such as 12.5 nM DAPGreen [35] and 125 nM DALGreen [36], were chosen to probe autophagosomes/autolysosomes and autolysosomes, respectively. After PBS washing, drug-treated cells were detected and quantified by flow cytometry.

4.9. Statistics

One-way ANOVA with Tukey’s HSD post-hoc test was performed by JMP®12 (SAS Institute Inc., Cary, NC, USA) to determine the significances between multiple comparisons. Treatments of non-overlapping notes showed significant results.

5. Conclusions

Manoalide, characterized originally as an antibiotic, was here repurposed for anti-cancer treatments and the preferential antiproliferation and apoptosis of oral cancer cells was monitored, while it showed little impact on normal cells [29,30]. However, ER stress commonly interplayed with apoptosis, but the involvement of ER stress with oral cancer cells following manoalide treatment has rarely been investigated.
The present study validates that manoalide triggers more ER stress in oral cancer cells than in normal cells. ER expansion and the aggresome accumulation and gene expressions of ER-stress-associated proteins were upregulated by manoalide in oral cancer cells, but they were slightly changed in normal cells. Moreover, the potential connections between ER stress, antiproliferation, apoptosis, and autophagy were explored. Utilizing ER stress inducer TG, the manoalide-triggered antiproliferation and apoptosis were further enhanced. This finding indicates that manoalide-triggered ER stress is vital in controlling antiproliferation, apoptosis, and autophagy of oral cancer cells, but they showed low changes in normal cells. Consequently, manoalide showed preferential ER stress induction and contributes to the preferential antiproliferation of oral cancer cells.

Author Contributions

Conceptualization, H.-W.C. and C.-Y.Y.; Data curation, S.-Y.P.; Formal analysis, S.-Y.P.; Methodology, J.-Y.T., K.-L.C., J.-P.S. and J.-H.J.; Supervision, H.-W.C. and C.-Y.Y.; Writing—original draft, S.-Y.P., T.-H.L. and H.-W.C.; Writing—review and editing, H.-W.C. and C.-Y.Y. All authors have read and agreed to the published version of the manuscript.

Funding

This study was partly supported by funds from the Ministry of Science and Technology (MOST 111-2320-B-037-015-MY3 and MOST 110-2314-B-037-074-MY3), the Kaohsiung Medical University (KMU-DK(A)112008), and the Kaohsiung Medical University Research Center (KMU-TC108A04).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Data are contained within the article.

Conflicts of Interest

The authors declare that there are no conflicts of interest among them.

References

  1. Siegel, R.L.; Miller, K.D.; Fuchs, H.E.; Jemal, A. Cancer statistics, 2022. CA Cancer J. Clin. 2022, 72, 7–33. [Google Scholar] [CrossRef] [PubMed]
  2. Chung, C.H.; Yang, Y.H.; Wang, T.Y.; Shieh, T.Y.; Warnakulasuriya, S. Oral precancerous disorders associated with areca quid chewing, smoking, and alcohol drinking in southern Taiwan. J. Oral Pathol. Med. 2005, 34, 460–466. [Google Scholar] [CrossRef] [PubMed]
  3. Awan, K.H.; Patil, S.; Habib, S.R.; Pejcic, A.; Zain, R.B. Evaluation of medicinal interventions for the management of oral submucous fibrosis: A systematic review of the literature. J. Contemp. Dent. Pract. 2014, 15, 812–817. [Google Scholar] [CrossRef] [PubMed]
  4. Lin, S.H.; Chen, M.K.; Chang, J.H.; Velmurugan, B.K.; Annamanedi, M.; Su, S.C.; Yeh, K.T.; Yang, S.F. Impact of polymorphisms in casein kinase 1 epsilon and environmental factors in oral cancer susceptibility. J. Cancer 2019, 10, 5065–5069. [Google Scholar] [CrossRef]
  5. Singh, R.; Sharma, M.; Joshi, P.; Rawat, D.S. Clinical status of anti-cancer agents derived from marine sources. Anticancer Agents Med. Chem. 2008, 8, 603–617. [Google Scholar] [CrossRef]
  6. Sithranga Boopathy, N.; Kathiresan, K. Anticancer drugs from marine flora: An overview. J. Oncol. 2010, 2010, 214186. [Google Scholar] [CrossRef] [Green Version]
  7. Farooqi, A.A.; Fayyaz, S.; Hou, M.F.; Li, K.T.; Tang, J.Y.; Chang, H.W. Reactive oxygen species and autophagy modulation in non-marine drugs and marine drugs. Mar. Drugs 2014, 12, 5408–5424. [Google Scholar] [CrossRef] [Green Version]
  8. Lee, M.G.; Liu, Y.C.; Lee, Y.L.; El-Shazly, M.; Lai, K.H.; Shih, S.P.; Ke, S.C.; Hong, M.C.; Du, Y.C.; Yang, J.C.; et al. Heteronemin, a marine sesterterpenoid-type metabolite, induces apoptosis in prostate LNcap cells via oxidative and ER stress combined with the inhibition of topoisomerase II and Hsp90. Mar. Drugs 2018, 16, 204. [Google Scholar] [CrossRef] [Green Version]
  9. Abdelaleem, E.R.; Samy, M.N.; Desoukey, S.Y.; Liu, M.; Quinn, R.J.; Abdelmohsen, U.R. Marine natural products from sponges (Porifera) of the order Dictyoceratida (2013 to 2019); a promising source for drug discovery. RSC Adv. 2020, 10, 34959–34976. [Google Scholar] [CrossRef]
  10. Elgoud Said, A.A.; Mahmoud, B.K.; Attia, E.Z.; Abdelmohsen, U.R.; Fouad, M.A. Bioactive natural products from marine sponges belonging to family Hymedesmiidae. RSC Adv. 2021, 11, 16179–16191. [Google Scholar] [CrossRef]
  11. Sakai, E.; Kato, H.; Rotinsulu, H.; Losung, F.; Mangindaan, R.E.; de Voogd, N.J.; Yokosawa, H.; Tsukamoto, S. Variabines A and B: New beta-carboline alkaloids from the marine sponge Luffariella variabilis. J. Nat. Med. 2014, 68, 215–219. [Google Scholar] [CrossRef]
  12. de Silva, E.D.; Scheuer, P.J. Manoalide, an antibiotic sesterterpenoid from the marine sponge Luffariella variabilis (polejaeff). Tetrahedron Lett. 1980, 21, 1611–1614. [Google Scholar] [CrossRef]
  13. Soriente, A.; De Rosa, M.M.; Scettri, A.; Sodano, G.; Terencio, M.C.; Paya, M.; Alcaraz, M.J. Manoalide. Curr. Med. Chem. 1999, 6, 415–431. [Google Scholar] [CrossRef]
  14. Kobayashi, J.; Zeng, C.M.; Ishibashi, M.; Sasaki, T. Luffariolides F and G, new manoalide derivatives from the Okinawan marine sponge Luffariella sp. J. Nat. Prod. 1993, 56, 436–439. [Google Scholar] [CrossRef]
  15. Kaweetripob, W.; Mahidol, C.; Tuntiwachwuttikul, P.; Ruchirawat, S.; Prawat, H. Cytotoxic sesterterpenes from Thai marine sponge Hyrtios erectus. Mar. Drugs 2018, 16, 474. [Google Scholar] [CrossRef] [Green Version]
  16. Fernandez, A.; Ordonez, R.; Reiter, R.J.; Gonzalez-Gallego, J.; Mauriz, J.L. Melatonin and endoplasmic reticulum stress: Relation to autophagy and apoptosis. J. Pineal Res. 2015, 59, 292–307. [Google Scholar] [CrossRef]
  17. Schwarz, D.S.; Blower, M.D. The endoplasmic reticulum: Structure, function and response to cellular signaling. Cell. Mol. Life Sci. 2016, 73, 79–94. [Google Scholar] [CrossRef] [Green Version]
  18. Ozcan, L.; Tabas, I. Role of endoplasmic reticulum stress in metabolic disease and other disorders. Annu. Rev. Med. 2012, 63, 317–328. [Google Scholar] [CrossRef] [Green Version]
  19. Limonta, P.; Moretti, R.M.; Marzagalli, M.; Fontana, F.; Raimondi, M.; Montagnani Marelli, M. Role of endoplasmic reticulum stress in the anticancer activity of natural compounds. Int. J. Mol. Sci. 2019, 20, 961. [Google Scholar] [CrossRef] [Green Version]
  20. Yadav, R.K.; Chae, S.W.; Kim, H.R.; Chae, H.J. Endoplasmic reticulum stress and cancer. J. Cancer Prev. 2014, 19, 75–88. [Google Scholar] [CrossRef]
  21. Yorimitsu, T.; Nair, U.; Yang, Z.; Klionsky, D.J. Endoplasmic reticulum stress triggers autophagy. J. Biol. Chem. 2006, 281, 30299–30304. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  22. Qin, L.; Wang, Z.; Tao, L.; Wang, Y. ER stress negatively regulates AKT/TSC/mTOR pathway to enhance autophagy. Autophagy 2010, 6, 239–247. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  23. Zeeshan, H.M.; Lee, G.H.; Kim, H.R.; Chae, H.J. Endoplasmic reticulum stress and associated ROS. Int. J. Mol. Sci. 2016, 17, 327. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  24. Burgos-Moron, E.; Abad-Jimenez, Z.; Maranon, A.M.; Iannantuoni, F.; Escribano-Lopez, I.; Lopez-Domenech, S.; Salom, C.; Jover, A.; Mora, V.; Roldan, I.; et al. Relationship between oxidative stress, ER stress, and inflammation in type 2 diabetes: The battle continues. J. Clin. Med. 2019, 8, 1385. [Google Scholar] [CrossRef] [Green Version]
  25. Kim, J.K.; Kang, K.A.; Ryu, Y.S.; Piao, M.J.; Han, X.; Oh, M.C.; Boo, S.J.; Jeong, S.U.; Jeong, Y.J.; Chae, S.; et al. Induction of endoplasmic reticulum stress via reactive oxygen species mediated by luteolin in melanoma cells. Anticancer Res. 2016, 36, 2281–2289. [Google Scholar]
  26. Tang, J.Y.; Ou-Yang, F.; Hou, M.F.; Huang, H.W.; Wang, H.R.; Li, K.T.; Fayyaz, S.; Shu, C.W.; Chang, H.W. Oxidative stress-modulating drugs have preferential anticancer effects—Involving the regulation of apoptosis, DNA damage, endoplasmic reticulum stress, autophagy, metabolism, and migration. Semin. Cancer Biol. 2019, 58, 109–117. [Google Scholar] [CrossRef]
  27. Lin, Y.; Jiang, M.; Chen, W.; Zhao, T.; Wei, Y. Cancer and ER stress: Mutual crosstalk between autophagy, oxidative stress and inflammatory response. Biomed. Pharmacother. 2019, 118, 109249. [Google Scholar] [CrossRef]
  28. Cheng, W.C.; Wen, Y.; Chiu, Y.S.; Chou, C.H.; Lim, C.J.; Lin, S.H.; Chang, J.M.; Lin, C.C. Pendulone induces apoptosis via the ROS-mediated ER-stress pathway in human non-small cell lung cancer cells. Toxicol. In Vitro 2022, 81, 105346. [Google Scholar] [CrossRef]
  29. Wang, H.R.; Tang, J.Y.; Wang, Y.Y.; Farooqi, A.A.; Yen, C.Y.; Yuan, S.F.; Huang, H.W.; Chang, H.W. Manoalide preferentially provides antiproliferation of oral cancer cells by oxidative stress-mediated apoptosis and DNA damage. Cancers 2019, 11, 1303. [Google Scholar] [CrossRef] [Green Version]
  30. Wang, H.R.; Chen, P.H.; Tang, J.Y.; Yen, C.Y.; Su, Y.C.; Huang, M.Y.; Chang, H.W. Manoalide shows mutual interaction between cellular and mitochondrial reactive species with apoptosis in oral cancer cells. Oxid. Med. Cell. Longev. 2021, 2021, 6667355. [Google Scholar] [CrossRef]
  31. Liu, X.D.; Ko, S.; Xu, Y.; Fattah, E.A.; Xiang, Q.; Jagannath, C.; Ishii, T.; Komatsu, M.; Eissa, N.T. Transient aggregation of ubiquitinated proteins is a cytosolic unfolded protein response to inflammation and endoplasmic reticulum stress. J. Biol. Chem. 2012, 287, 19687–19698. [Google Scholar] [CrossRef] [Green Version]
  32. Wang, X.; Shi, Q.; Xu, K.; Gao, C.; Chen, C.; Li, X.L.; Wang, G.R.; Tian, C.; Han, J.; Dong, X.P. Familial CJD associated PrP mutants within transmembrane region induced Ctm-PrP retention in ER and triggered apoptosis by ER stress in SH-SY5Y cells. PLoS ONE 2011, 6, e14602. [Google Scholar] [CrossRef]
  33. Petty, R.D.; Sutherland, L.A.; Hunter, E.M.; Cree, I.A. Comparison of MTT and ATP-based assays for the measurement of viable cell number. J. Biolumin. Chemilumin. 1995, 10, 29–34. [Google Scholar] [CrossRef]
  34. Maehara, Y.; Anai, H.; Tamada, R.; Sugimachi, K. The ATP assay is more sensitive than the succinate-dehydrogenase inhibition test for predicting cell viability. Eur. J. Cancer Clin. Oncol. 1987, 23, 273–276. [Google Scholar] [CrossRef]
  35. Hu, L.; Zhang, T.; Liu, D.; Guan, G.; Huang, J.; Proksch, P.; Chen, X.; Lin, W. Notoamide-type alkaloid induced apoptosis and autophagy via a P38/JNK signaling pathway in hepatocellular carcinoma cells. RSC Adv. 2019, 9, 19855–19868. [Google Scholar] [CrossRef] [Green Version]
  36. Sasabe, E.; Tomomura, A.; Kitamura, N.; Yamamoto, T. Metal nanoparticles-induced activation of NLRP3 inflammasome in human oral keratinocytes is a possible mechanism of oral lichenoid lesions. Toxicol. In Vitro 2020, 62, 104663. [Google Scholar] [CrossRef]
  37. Bhattarai, K.R.; Riaz, T.A.; Kim, H.R.; Chae, H.J. The aftermath of the interplay between the endoplasmic reticulum stress response and redox signaling. Exp. Mol. Med. 2021, 53, 151–167. [Google Scholar] [CrossRef]
  38. Yuan, X.; Wang, B.; Yang, L.; Zhang, Y. The role of ROS-induced autophagy in hepatocellular carcinoma. Clin. Res. Hepatol. Gastroenterol. 2018, 42, 306–312. [Google Scholar] [CrossRef]
  39. Backer, J.M.; Krivoshein, A.V.; Hamby, C.V.; Pizzonia, J.; Gilbert, K.S.; Ray, Y.S.; Brand, H.; Paton, A.W.; Paton, J.C.; Backer, M.V. Chaperone-targeting cytotoxin and endoplasmic reticulum stress-inducing drug synergize to kill cancer cells. Neoplasia 2009, 11, 1165–1173. [Google Scholar] [CrossRef]
  40. Wu, L.; Huang, X.; Kuang, Y.; Xing, Z.; Deng, X.; Luo, Z. Thapsigargin induces apoptosis in adrenocortical carcinoma by activating endoplasmic reticulum stress and the JNK signaling pathway: An in vitro and in vivo study. Drug Des. Dev. Ther. 2019, 13, 2787–2798. [Google Scholar] [CrossRef] [Green Version]
  41. Huang, F.; Wang, P.; Wang, X. Thapsigargin induces apoptosis of prostate cancer through cofilin-1 and paxillin. Oncol. Lett. 2018, 16, 1975–1980. [Google Scholar] [CrossRef] [PubMed]
  42. Pan, M.Y.; Shen, Y.C.; Lu, C.H.; Yang, S.Y.; Ho, T.F.; Peng, Y.T.; Chang, C.C. Prodigiosin activates endoplasmic reticulum stress cell death pathway in human breast carcinoma cell lines. Toxicol. Appl. Pharmacol. 2012, 265, 325–334. [Google Scholar] [CrossRef] [PubMed]
  43. Sun, Y.; Pu, L.Y.; Lu, L.; Wang, X.H.; Zhang, F.; Rao, J.H. N-acetylcysteine attenuates reactive-oxygen-species-mediated endoplasmic reticulum stress during liver ischemia-reperfusion injury. World J. Gastroenterol. 2014, 20, 15289–15298. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  44. Chiu, C.C.; Chen, Y.C.; Bow, Y.D.; Chen, J.Y.; Liu, W.; Huang, J.L.; Shu, E.D.; Teng, Y.N.; Wu, C.Y.; Chang, W.T. diTFPP, a phenoxyphenol, sensitizes hepatocellular carcinoma cells to c2-ceramide-induced autophagic stress by increasing oxidative stress and ER stress accompanied by LAMP2 hypoglycosylation. Cancers 2022, 14, 2528. [Google Scholar] [CrossRef]
  45. Su, M.; Mei, Y.; Sinha, S. Role of the crosstalk between autophagy and apoptosis in cancer. J. Oncol. 2013, 2013, 102735. [Google Scholar] [CrossRef] [Green Version]
  46. Holczer, M.; Marton, M.; Kurucz, A.; Banhegyi, G.; Kapuy, O. A comprehensive systems biological study of autophagy-apoptosis crosstalk during endoplasmic reticulum stress. BioMed Res. Int. 2015, 2015, 319589. [Google Scholar] [CrossRef] [Green Version]
  47. Song, S.; Tan, J.; Miao, Y.; Li, M.; Zhang, Q. Crosstalk of autophagy and apoptosis: Involvement of the dual role of autophagy under ER stress. J. Cell. Physiol. 2017, 232, 2977–2984. [Google Scholar] [CrossRef]
  48. Bhutia, S.K.; Dash, R.; Das, S.K.; Azab, B.; Su, Z.Z.; Lee, S.G.; Grant, S.; Yacoub, A.; Dent, P.; Curiel, D.T.; et al. Mechanism of autophagy to apoptosis switch triggered in prostate cancer cells by antitumor cytokine melanoma differentiation-associated gene 7/interleukin-24. Cancer Res. 2010, 70, 3667–3676. [Google Scholar] [CrossRef] [Green Version]
  49. Lee, Y.S.; Lee, D.H.; Choudry, H.A.; Bartlett, D.L.; Lee, Y.J. Ferroptosis-induced endoplasmic reticulum stress: Cross-talk between ferroptosis and apoptosis. Mol. Cancer Res. 2018, 16, 1073–1076. [Google Scholar] [CrossRef] [Green Version]
  50. Kasten, F.H.; Pineda, L.F.; Schneider, P.E.; Rawls, H.R.; Foster, T.A. Biocompatibility testing of an experimental fluoride releasing resin using human gingival epithelial cells in vitro. In Vitro Cell. Dev. Biol. 1989, 25, 57–62. [Google Scholar] [CrossRef]
  51. Kasten, F.H.; Soileau, K.; Meffert, R.M. Quantitative evaluation of human gingival epithelial cell attachment to implant surfaces in vitro. Int. J. Periodontics Restor. Dent. 1990, 10, 68–79. [Google Scholar]
  52. Chang, H.H.; Guo, M.K.; Kasten, F.H.; Chang, M.C.; Huang, G.F.; Wang, Y.L.; Wang, R.S.; Jeng, J.H. Stimulation of glutathione depletion, ROS production and cell cycle arrest of dental pulp cells and gingival epithelial cells by HEMA. Biomaterials 2005, 26, 745–753. [Google Scholar] [CrossRef]
  53. Zieniewska, I.; Maciejczyk, M.; Zalewska, A. The effect of selected dental materials used in conservative dentistry, endodontics, surgery, and orthodontics as well as during the periodontal treatment on the redox balance in the oral cavity. Int. J. Mol. Sci. 2020, 21, 9684. [Google Scholar] [CrossRef]
  54. Hsieh, P.L.; Liao, Y.W.; Hsieh, C.W.; Chen, P.N.; Yu, C.C. Soy isoflavone genistein impedes cancer stemness and mesenchymal transition in head and neck cancer through activating miR-34a/RTCB axis. Nutrients 2020, 12, 1924. [Google Scholar] [CrossRef]
  55. Chen, S.Y.; Chang, Y.L.; Liu, S.T.; Chen, G.S.; Lee, S.P.; Huang, S.M. Differential cytotoxicity mechanisms of copper complexed with disulfiram in oral cancer cells. Int. J. Mol. Sci. 2021, 22, 3711. [Google Scholar] [CrossRef]
  56. Chan, W.H.; Shiao, N.H.; Lu, P.Z. CdSe quantum dots induce apoptosis in human neuroblastoma cells via mitochondrial-dependent pathways and inhibition of survival signals. Toxicol. Lett. 2006, 167, 191–200. [Google Scholar] [CrossRef]
  57. Hung, J.H.; Chen, C.Y.; Omar, H.A.; Huang, K.Y.; Tsao, C.C.; Chiu, C.C.; Chen, Y.L.; Chen, P.H.; Teng, Y.N. Reactive oxygen species mediate Terbufos-induced apoptosis in mouse testicular cell lines via the modulation of cell cycle and pro-apoptotic proteins. Environ. Toxicol. 2016, 31, 1888–1898. [Google Scholar] [CrossRef]
  58. Huang, C.H.; Yeh, J.M.; Chan, W.H. Hazardous impacts of silver nanoparticles on mouse oocyte maturation and fertilization and fetal development through induction of apoptotic processes. Environ. Toxicol. 2018, 33, 1039–1049. [Google Scholar] [CrossRef]
  59. Wang, T.S.; Lin, C.P.; Chen, Y.P.; Chao, M.R.; Li, C.C.; Liu, K.L. CYP450-mediated mitochondrial ROS production involved in arecoline N-oxide-induced oxidative damage in liver cell lines. Environ. Toxicol. 2018, 33, 1029–1038. [Google Scholar] [CrossRef]
  60. Mocan, T.; Matea, C.; Tabaran, F.; Iancu, C.; Orasan, R.; Mocan, L. In vitro administration of gold nanoparticles functionalized with MUC-1 protein fragment generates anticancer vaccine response via macrophage activation and polarization mechanism. J. Cancer 2015, 6, 583–592. [Google Scholar] [CrossRef]
  61. Hirata, Y.; Motoyama, M.; Kimura, S.; Takashima, M.; Ikawa, T.; Oh-Hashi, K.; Kamatari, Y.O. Artepillin C, a major component of Brazilian green propolis, inhibits endoplasmic reticulum stress and protein aggregation. Eur. J. Pharmacol. 2021, 912, 174572. [Google Scholar] [CrossRef] [PubMed]
  62. Chang, H.W.; Yen, C.Y.; Chen, C.H.; Tsai, J.H.; Tang, J.Y.; Chang, Y.T.; Kao, Y.H.; Wang, Y.Y.; Yuan, S.F.; Lee, S.Y. Evaluation of the mRNA expression levels of integrins alpha3, alpha5, beta1 and beta6 as tumor biomarkers of oral squamous cell carcinoma. Oncol. Lett. 2018, 16, 4773–4781. [Google Scholar] [PubMed] [Green Version]
  63. Fujii, Y.; Yoshihashi, K.; Suzuki, H.; Tsutsumi, S.; Mutoh, H.; Maeda, S.; Yamagata, Y.; Seto, Y.; Aburatani, H.; Hatakeyama, M. CDX1 confers intestinal phenotype on gastric epithelial cells via induction of stemness-associated reprogramming factors SALL4 and KLF5. Proc. Natl. Acad. Sci. USA 2012, 109, 20584–20589. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  64. Laddha, N.C.; Dwivedi, M.; Mansuri, M.S.; Singh, M.; Patel, H.H.; Agarwal, N.; Shah, A.M.; Begum, R. Association of neuropeptide Y (NPY), interleukin-1B (IL1B) genetic variants and correlation of IL1B transcript levels with vitiligo susceptibility. PLoS ONE 2014, 9, e107020. [Google Scholar] [CrossRef] [Green Version]
  65. Livak, K.J.; Schmittgen, T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 2001, 25, 402–408. [Google Scholar] [CrossRef]
  66. Peng, S.Y.; Yen, C.Y.; Lan, T.H.; Jeng, J.H.; Tang, J.Y.; Chang, H.W. Combined treatment (ultraviolet-C/physapruin A) enhances antiproliferation and oxidative-stress-associated mechanism in oral cancer cells. Antioxidants 2022, 11, 2227. [Google Scholar] [CrossRef]
  67. Yeh, C.C.; Tseng, C.N.; Yang, J.I.; Huang, H.W.; Fang, Y.; Tang, J.Y.; Chang, F.R.; Chang, H.W. Antiproliferation and induction of apoptosis in Ca9-22 oral cancer cells by ethanolic extract of Gracilaria tenuistipitata. Molecules 2012, 17, 10916–10927. [Google Scholar] [CrossRef] [Green Version]
  68. Shiau, J.P.; Chuang, Y.T.; Tang, J.Y.; Chen, S.R.; Hou, M.F.; Jeng, J.H.; Cheng, Y.B.; Chang, H.W. Antiproliferation effects of marine-sponge-derived methanol extract of Theonella swinhoei in oral cancer cells in vitro. Antioxidants 2022, 11, 1982. [Google Scholar] [CrossRef]
Ijms 24 03987 g001 550
Figure 1. Manoalide causes ER expansion of oral cancer cells. (A,B) ER expansion analyses. Oral cancer (CAL 27 and Ca9-22) and normal oral (SG) cells received control, 5, and 10 μM manoalide treatments for 24 h. (+) was marked for ER expansion (+) (%). (C,D) NAC effects on time course changes of manoalide-induced ER expansion. Following NAC (10 mM for 1 h), cells received manoalide treatment (control and 10 μM) for 12 and 24 h, i.e., NAC/Mano. Data, means ± SDs (n = 3). Multiple comparisons were analyzed for the same cell lines. Treatments marked with different notes indicate a significant difference (p < 0.05 to 0.0001).
Figure 1. Manoalide causes ER expansion of oral cancer cells. (A,B) ER expansion analyses. Oral cancer (CAL 27 and Ca9-22) and normal oral (SG) cells received control, 5, and 10 μM manoalide treatments for 24 h. (+) was marked for ER expansion (+) (%). (C,D) NAC effects on time course changes of manoalide-induced ER expansion. Following NAC (10 mM for 1 h), cells received manoalide treatment (control and 10 μM) for 12 and 24 h, i.e., NAC/Mano. Data, means ± SDs (n = 3). Multiple comparisons were analyzed for the same cell lines. Treatments marked with different notes indicate a significant difference (p < 0.05 to 0.0001).
Ijms 24 03987 g001
Ijms 24 03987 g002 550
Figure 2. Manoalide causes the aggresome accumulation of oral cancer cells. (A,B) Aggresome analyses. Oral cancer and normal oral (SG) cells received control, 5, and 10 μM manoalide treatments for 24 h. (+) was marked for aggresome (+) (%). (C,D) NAC effects on time course changes of manoalide-induced aggresome. Following NAC (10 mM for 1 h), cells received manoalide treatment (control and 10 μM) for 12 and 24 h, i.e., NAC/Mano. Data, means ± SDs (n = 3). Multiple comparisons were analyzed for the same cell lines. Treatments marked with different notes indicate significant differences (p < 0.05 to 0.0001).
Figure 2. Manoalide causes the aggresome accumulation of oral cancer cells. (A,B) Aggresome analyses. Oral cancer and normal oral (SG) cells received control, 5, and 10 μM manoalide treatments for 24 h. (+) was marked for aggresome (+) (%). (C,D) NAC effects on time course changes of manoalide-induced aggresome. Following NAC (10 mM for 1 h), cells received manoalide treatment (control and 10 μM) for 12 and 24 h, i.e., NAC/Mano. Data, means ± SDs (n = 3). Multiple comparisons were analyzed for the same cell lines. Treatments marked with different notes indicate significant differences (p < 0.05 to 0.0001).
Ijms 24 03987 g002
Ijms 24 03987 g003 550
Figure 3. Manoalide causes ER stress gene expression of oral cancer cells. (A) Relative mRNA expressions (fold activation) of ER stress genes. Oral cancer and normal oral (SG) cells received control, 5, and 10 μM manoalide treatments for 24 h. Data, means ± SDs (n = 3). Multiple comparisons were analyzed for the same cell lines. For the same gene, treatments marked with different notes indicate a significant difference (p < 0.05 to 0.0001). (B) NAC effects on Western blotting for ER stress signaling. Following NAC (10 mM for 1 h), cells received manoalide treatment (control, 5, and 10 μM) for 24 h, i.e., NAC/Mano.
Figure 3. Manoalide causes ER stress gene expression of oral cancer cells. (A) Relative mRNA expressions (fold activation) of ER stress genes. Oral cancer and normal oral (SG) cells received control, 5, and 10 μM manoalide treatments for 24 h. Data, means ± SDs (n = 3). Multiple comparisons were analyzed for the same cell lines. For the same gene, treatments marked with different notes indicate a significant difference (p < 0.05 to 0.0001). (B) NAC effects on Western blotting for ER stress signaling. Following NAC (10 mM for 1 h), cells received manoalide treatment (control, 5, and 10 μM) for 24 h, i.e., NAC/Mano.
Ijms 24 03987 g003
Ijms 24 03987 g004 550
Figure 4. ER stress inducer (TG) enhances the antiproliferative effect of manoalide on oral cancer cells. (A) Cell viability of TG and/or manoalide was assessed by ATP assay. Cells were co-treated with TG (0.01 μM) and manoalide (0, 5, and 10 μM) for 24 h. (B,C) NAC effects on cell viability of TG and/or manoalide (Mano) were assessed by ATP and MTS assays. Following NAC (10 mM for 1 h), cells received TG and/or manoalide treatment (control, TG 0.01 μM, and/or Mano 10 μM) for 24 h. Data, means ± SDs (n = 3). Multiple comparisons were analyzed for the same cell lines. Treatments marked with different notes indicate a significant difference (p < 0.05 to 0.0001).
Figure 4. ER stress inducer (TG) enhances the antiproliferative effect of manoalide on oral cancer cells. (A) Cell viability of TG and/or manoalide was assessed by ATP assay. Cells were co-treated with TG (0.01 μM) and manoalide (0, 5, and 10 μM) for 24 h. (B,C) NAC effects on cell viability of TG and/or manoalide (Mano) were assessed by ATP and MTS assays. Following NAC (10 mM for 1 h), cells received TG and/or manoalide treatment (control, TG 0.01 μM, and/or Mano 10 μM) for 24 h. Data, means ± SDs (n = 3). Multiple comparisons were analyzed for the same cell lines. Treatments marked with different notes indicate a significant difference (p < 0.05 to 0.0001).
Ijms 24 03987 g004
Ijms 24 03987 g005 550
Figure 5. ER stress inducer (TG) enhances the caspase 3/7 activation of manoalide on oral cancer cells. Cells were co-treated with TG (0.01 μM) and manoalide (0, 5, and 10 μM) for 24 h. Data, means ± SDs (n = 3). Multiple comparisons were analyzed for the same cell lines. Treatments marked with different notes indicate a significant difference (p < 0.05 to 0.0001).
Figure 5. ER stress inducer (TG) enhances the caspase 3/7 activation of manoalide on oral cancer cells. Cells were co-treated with TG (0.01 μM) and manoalide (0, 5, and 10 μM) for 24 h. Data, means ± SDs (n = 3). Multiple comparisons were analyzed for the same cell lines. Treatments marked with different notes indicate a significant difference (p < 0.05 to 0.0001).
Ijms 24 03987 g005
Ijms 24 03987 g006 550
Figure 6. ER stress inducer (TG) enhances the autophagy of manoalide in oral cancer cells. (A,B) Autophagy change of TG and/or manoalide was assessed by DAPGreen and DALGreen assay. Cells were co-treated with TG (0.01 μM) or/and manoalide (10 μM) for 24 h. Data, means ± SDs (n = 3). Multiple comparisons were analyzed for the same cell lines. Treatments marked with different notes indicate a significant difference (p < 0.05 to 0.0001).
Figure 6. ER stress inducer (TG) enhances the autophagy of manoalide in oral cancer cells. (A,B) Autophagy change of TG and/or manoalide was assessed by DAPGreen and DALGreen assay. Cells were co-treated with TG (0.01 μM) or/and manoalide (10 μM) for 24 h. Data, means ± SDs (n = 3). Multiple comparisons were analyzed for the same cell lines. Treatments marked with different notes indicate a significant difference (p < 0.05 to 0.0001).
Ijms 24 03987 g006
Table
Table 1. Primers of ER-stress-related genes.
Table 1. Primers of ER-stress-related genes.
GenesForward Primers (5′→3′)Reverse primers (5′→3′)Accession Numbers
BIPTGATCAAGATACAGGTGACCTGGGTCTTTCACCTTCATAGACCTTGATNM_005347.5
PERKTCATCCTCACAGGCAAAGGAAGAGCCAATTCCCTATTGGGGANM_004836.7
IRE1αAATTGTGTACCGGGGCATGTTGCTCCACATACTCTTGCAGGNM_001433.5
ATF6ACAGAGTCTCTCAGGTTAAATCATGGAGTTCCTGCTGATACTACTAGTGGNM_007348.4
GAPDHCCTCAACTACATGGTTTACATGTTCC CAAATGAGCCCCAGCCTTCT NM_002046.7
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.

Share and Cite

MDPI and ACS Style

Peng, S.-Y.; Tang, J.-Y.; Lan, T.-H.; Shiau, J.-P.; Chen, K.-L.; Jeng, J.-H.; Yen, C.-Y.; Chang, H.-W. Oxidative-Stress-Mediated ER Stress Is Involved in Regulating Manoalide-Induced Antiproliferation in Oral Cancer Cells. Int. J. Mol. Sci. 2023, 24, 3987. https://doi.org/10.3390/ijms24043987

AMA Style

Peng S-Y, Tang J-Y, Lan T-H, Shiau J-P, Chen K-L, Jeng J-H, Yen C-Y, Chang H-W. Oxidative-Stress-Mediated ER Stress Is Involved in Regulating Manoalide-Induced Antiproliferation in Oral Cancer Cells. International Journal of Molecular Sciences. 2023; 24(4):3987. https://doi.org/10.3390/ijms24043987

Chicago/Turabian Style

Peng, Sheng-Yao, Jen-Yang Tang, Ting-Hsun Lan, Jun-Ping Shiau, Kuan-Liang Chen, Jiiang-Huei Jeng, Ching-Yu Yen, and Hsueh-Wei Chang. 2023. "Oxidative-Stress-Mediated ER Stress Is Involved in Regulating Manoalide-Induced Antiproliferation in Oral Cancer Cells" International Journal of Molecular Sciences 24, no. 4: 3987. https://doi.org/10.3390/ijms24043987

APA Style

Peng, S. -Y., Tang, J. -Y., Lan, T. -H., Shiau, J. -P., Chen, K. -L., Jeng, J. -H., Yen, C. -Y., & Chang, H. -W. (2023). Oxidative-Stress-Mediated ER Stress Is Involved in Regulating Manoalide-Induced Antiproliferation in Oral Cancer Cells. International Journal of Molecular Sciences, 24(4), 3987. https://doi.org/10.3390/ijms24043987

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop