5-[4-(tert-Butyl)cyclohexylidene]-2-thioxothiazolidin-4-one

Round 1

Reviewer 1 Report

Molecules

Manuscript ID: molbank-1340149

Title: “5-(4-(tert-Butyl)cyclohexylidene)-2-thioxothiazolidin-4-one”

The short note involves the synthesis of 5-(4-(tert-butyl)cyclohexylidene)-2-thioxothiazolidin-4-one using a Knoevenagel reaction type and the investigation of their antimicrobial activity.

The preparation is interesting, mas needs improve some questions:

1. In the page 2, Schem 1, the authors could number structure 3 to better fellow the structural elucidation.
2. Is the compound 3 a new compound? The authors must be indicating.
3. The authors could show the 1H and 13C NMR chemical shifts to each atom in the compound 3.
4. In the Table 2 the author must put the result of positive control.
5. The MIC values must be obtained at least to M. luteus. By stating that 3 is promising.
6. At the end of the results, the authors suggest that the observed activity would be due to the destruction of the aminoglycan against M. luteus. For this suggestion the authors should present some experimental or theoretical evidence.

Author Response

Dear Reviewer,

We would like to thank you for the review of our article and extremely valuable and constructive comments that helped to improve this manuscript. We agree with your suggestions, and relevant corrections have been incorporated in revised version of the manuscript (please see through the text of revised manuscript marked with green).

1. Reviewer’ recommendation: In the page 2, Schem 1, the authors could number structure 3 to better fellow the structural elucidation.

We agree with recommendation and prepared the Figure of numbering of compound 3, but we would like to ask to put mentioned Figure in the Supplementary part as such information in this case is more technical than scientific data

2. Reviewer’ recommendation: Is the compound 3 a new compound? The authors must be indicating.

Compound 3 is new, early undescribed in the chemical literature and we have added appropriate remark in the text in the Abstract

3. Reviewer’ recommendation: The authors could show the 1H and 13C NMR chemical shifts to each atom in the compound 3.

We have added full description of 1H and 13C NMR data in the text in the chapter “2.1. Synthesis of the Title Compound 3”

4. Reviewer’ recommendation: In the Table 2 the author must put the result of positive control.

The answer: Done.

We added vancomycin as a positive control in Table 2. We had these data but decided not to put them in the manuscript due to the irrelevance of comparison of growth inhibition diameters of tested compound and antibiotics because the diameter of growth inhibition considerably depends on molecular weight and may be different for different classes.

5. Reviewer’ recommendation: The MIC values must be obtained at least to M. luteus. By stating that 3 is promising.

The answer: It is in the manuscript. Highlight it and transfer after Table 2 and added more explanation.

6. Reviewer’ recommendation: At the end of the results, the authors suggest that the observed activity would be due to the destruction of the aminoglycan against M. luteus. For this suggestion the authors should present some experimental or theoretical evidence.

The answer: Thank you for your comment. We added reference [16] and short evidence based on different patterns of M.luteus antibiotic resistance with suggestions for future docking analysis.

Yours faithfully

Roman Lesyk

Round 2

Reviewer 1 Report

The authors answered all the proposed questions. Thus, I consider that the article presents a great improvement and can, in my opinion, be published. 

Author Response

Dear Reviewer,

We would like to thank you for the review of our article and extremely valuable and constructive comments that helped to improve this manuscript.