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Article

Preparation and Electrochemical Characterization of a Carbon Ceramic Electrode Modified with Ferrocenecarboxylic Acid

by
Tatiane Skeika
1,
Cristiane R. Zuconelli
1,
Sergio T. Fujiwara
2 and
Christiana A. Pessoa
1,*
1
Department of Chemistry, Universidade Estadual Ponta Grossa, Avenida Carlos Cavalcanti 4748, Uvaranas, Ponta Grossa, Brazil
2
Department of Chemistry, CEDETEG—Universidade Estadual do Centro-Oeste, Rua Presidente Zacarias 875, Guarapuava, Brazil
*
Author to whom correspondence should be addressed.
Sensors 2011, 11(2), 1361-1374; https://doi.org/10.3390/s110201361
Submission received: 3 December 2010 / Revised: 4 January 2011 / Accepted: 17 January 2011 / Published: 25 January 2011
(This article belongs to the Section Chemical Sensors)
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Abstract

: The present paper describes the characterization of a carbon ceramic electrode modified with ferrocenecarboxylic acid (designated as CCE/Fc) by electrochemical techniques and its detection ability for dopamine. From cyclic voltammetric experiments, it was observed that the CCE/Fc presented a redox pair at Epa = 405 mV and Epc = 335 mV (ΔE = 70 mV), related to the ferrocene/ferrocenium process. Studies showed a considerably increase in the redox currents at the same oxidation potential of ferrocene (Epa = 414 mV vs. Ag/AgCl) in the presence of dopamine (DA), differently from those observed when using only the unmodified CCE, in which the anodic peak increase was considerably lower. From SWV experiments, it was observed that the AA (ascorbic acid) oxidation at CCE/Fc occurred in a different potential than the DA oxidation (with a peak separation of approximately 200 mV). Moreover, CCE/Fc did not respond to different AA concentrations, indicating that it is possible to determine DA without the AA interference with this electrode.

Graphical Abstract

1. Introduction

The construction of electrodes using porous materials such as carbon ceramic electrodes (CCE), has seen a great development since the last decade [1–5]. The CCEs are a class of materials with high electrical conductivity which were firstly described by Lev et al. [6]. These electrodes are basically constructed by doping a silica matrix obtained by the sol-gel method with powdered carbon such as graphite, or other carbon materials (carbon nanotubes and glassy carbon) [7,8]. The advantage of using these materials compared to other carbon-based electrodes is the combination of the sol-gel process properties (such as high surface area) and conductivity of the carbon materials, thus enabling one to obtain a renewable surface electrode similar to a carbon paste electrode, but more robust and with higher stability [9–11].

An alternative to increase the application of carbon ceramic electrodes as electrochemical sensors is the modification of these materials using electron mediator species, such as organic or inorganic complexes and enzymes [12–14]. These mediators can provide new interesting features to these electrodes, such as the shift of peak potential of the analyte species to less positive potentials, thus increasing the sensitivity of the electrode [15–17]. Recently, a CCE modified with SnO2 and cobalt phthalocyanine was described. The obtained material resulted in a homogeneous material with good dispersion, which was applied to oxalic acid determination at 0.84 V (SCE) [18]. In this context, Salimi and Abdi constructed an amperometric sensor for hydrazine and hydroxylamine detection based on CCE modification with powdered nickel and subsequent deposition film of nickel hexacyanoferrate. The analytical parameters obtained for the modified CCE showed better results than those obtained for the unmodified CCE, justifying the advantage of electrode modification for determination of species of environmental interest [19]. Another interesting application for the carbon ceramic composite is related to the construction of biosensors. The detection of glucose was proposed by Tian et al., who developed a sensor consisting of polypyrrole (ppy) electrochemical deposition in the presence of glucose oxidase on the surface of a CCE with HRP incorporated during the sol-gel process. This biosensor has shown considerable sensibility to the analyte, with a high stability within the period of three weeks [20].

Ferrocene-derived compounds are examples of electrons mediators highly used in electrochemical systems and also in enzymatic reactions [21,22]. ascorbate determination [23]. dopamine [24]. pH and oxygen sensing [25]. Fernandez and Carrero reported a glassy carbon chemically modified using surfactant/clay films, containing ferrocenedicarboxylic acid. The results showed that low concentrations of ascorbic acid and uric acid are easily oxidized by the proposed electrode [26].

This paper reports the development of a CCE modified with ferrocenecarboxylic acid (CCE/Fc). The electrode was characterized by voltammetric techniques and applied as an electrochemical sensor for dopamine, which is an important neurotransmitter and is related to several diseases such as schizophrenia and parkinsonism [27,28]. Therefore, the development of methods for the quantification of dopamine in blood and biological fluids is the subject of intense current investigation [29–31]. The importance of using a carboxylated ferrocene derivative as a mediator for DA detection is related to the fact that the carboxylic groups depending of the solution pH could facilitate the interaction between this analyte and the electrode surface. Furthermore, the combination of such mediator with carbon ceramic composite electrodes can provide interesting properties and therefore increase the number of technological applications of these materials, especially in the field of electrochemical sensors.

2. Experimental Section

2.1. Preparation of Carbon Ceramic Electrode

The CCE/Fc was prepared according to the procedure previously described by Lev and co-workers [6]. The hydrolyzed mixture (where groups of the alkoxide precursor are converted to silanol groups) consisted of 1.5 mL of methanol (Aldrich), 1.2 mL of precursor, MTMOS (methyltrimethoxysilane) (Aldrich) and 15 μL of 12 mol L−1 HCl (Synth). To this mixture, three different quantities of ferrocenocarboxilic acid (2.5, 5.0 and 10 mg) and 1.8 g of graphite (Fluka) were added. The non-modified CCE was prepared by using the same experimental procedure used for CCE/Fc, without the ferrocenocarboxylic acid mixing step. The resulting sol was introduced into a glass tube (exposed area of approximately 0.2 cm2). A nickel-chromium (Ni-Cr) wire was inserted for electrical contact. The electrodes were subjected to the drying process at room temperature during one week.

2.2. Electrochemical Studies

The electrochemical measurements were carried out in a PalmSens potentiostat, connected to a computer for data acquisition, with the conventional three electrode system: platinum counter electrode, reference electrode (Ag/AgCl), and working electrodes (CCE and CCE/Fc).

The electrochemical characterization was performed by cyclic voltammetry, in supporting electrolyte (NaCl 0.5 mol L−1, pH = 6.0), using a 10 mL capacity electrochemical cell varying the scan rate (in the range of 10–100 mVs−1). The electrode response in presence of dopamine (DA) was also studied by cyclic voltammetry and square wave voltammetry in 0.1 mol L−1 phosphate buffer (pH = 7.0), which was used as supporting electrolyte. The dopamine solution was daily prepared in a concentration of 10 mmol L−1. Square wave voltammetry experiments were also evaluated in presence of DA and AA in different concentrations in the range from 1.0 to 2.5 μmol L−1. Studies in different pH (pH = 1.0 to 7.0) were evaluated using NaCl 0.5 mol L−1 solution. The adjustment of pH was carried out by NaOH or HCl addition.

2.3. Scanning Electron Microscopy and EDS Mapping

Morphological characterization of CCE/Fc was carried out by the techniques of scanning electron microscopy and energy dispersive spectroscopy (SEM and EDS), using a Shimadzu SSX-550 instrument. The samples were fixed on the sample holder using double-faced conductive graphite tape, without the need of a pretreatment, since the carbon ceramic has conductive properties. The CCE/Fc micrographs were obtained with a magnification of 500 X and 1,500 X in the range of 20 and 100 μm.

3. Results and Discussion

3.1. Scanning Electron Microscopy and EDS Mapping

Figure 1 shows the energy dispersive scanning images of Fe in CCE/Fc within magnifications of 500 X and 1,500 X (Figure 1A,B) and the corresponding EDS analysis (Figure 1C). From, the EDS images, it can be observed that the components are homogeneously dispersed in the sample but with some agglomerations of segregated ferrocenecarboxylic molecules. In order to confirm that the bright regions of the obtained EDS images were related to the ferrocenecarboxylic particles, a quantitative microanalysis of the highlighted region (circle with a dotted line in Figure 1B) was realized. The EDS analysis indicated a 72.38% w/w of Fe in the sample (Figure 1C).

3.2. Electrochemical Studies of the CCE/Fc

3.2.1. Electrochemical Characterization of the CCE/Fc

The carbon ceramic electrode was modified with three different amounts of ferrocene (0.0025 g; 0.005 g and 0.010 g), and designated as CCE/Fc. From the cyclic voltammetric studies, it was verified that only the CCE modified with the highest quantity of ferrocene presented redox peaks, with Epa = 405 mV and Epc = 335 mV (ΔE = 70 mV), as shown in Figure 2. These peaks are probably related to the ferrocene/ferrocenium redox process, since the non modified CCE did not present any redox peaks [26].

Voltammetric studies at different scan rates resulted in a linear relation between the anodic peak current values and the scan rate (Figure 3), which is a characteristic behavior for confined species in the bulk of the electrode, indicating that the electroactive species are strongly entrapped in the electrode surface [32].

In order to verify the stability of the modified electrode, consecutive voltammograms were obtained in the presence only of the electrolyte solution at a fixed scan rate (ν = 50 mV s−1). The voltammetric profile was analyzed as a function of the anodic current peak values (Figure 4). After repeated cycles (approximately 100), the current response of the bulk-modified carbon ceramic electrode did not decrease substantially (with a 0.22% variation, shown in Figure 4), indicating that the modified CCE is quite stable and can be used as an electrochemical sensor. The reproducibility of the electrode response was also studied as a function of the days (in the electrolyte solution) for one month. In this case, the Ipa variation was of 1.42 % and for Epa 0.18%, showing that the electrode holds its efficiency within this period of time.

The pH influence in the electrochemical response of the CCE/Fc was also verified. It was observed that with the increase of pH from 2 to 7, the peak potentials negatively shift from 520 to approximately 420 mV (figure not shown). However in this pH range, the peak currents remained practically constant. This can be explained considering that the ferrocenecarboxylic acid redox reaction involves protons [33], with a pKa = 4.2. When the pH decreases, it becomes more difficult to oxidize the ferrocenecarboxylic and peak potentials are shifted to more positive values.

3.2.2. Electrochemical Studies of the CCE/Fc in Presence of Dopamine

In order to verify the possibility of using this modified electrode as an electrochemical sensor, studies in presence of dopamine (DA) were carried out. The voltammograms obtained in the absence of DA, presented only the redox peaks related to the redox process of ferrocenecarboxylic acid. However, after addition of DA in the electrolyte solution, a considerable increase in the redox currents was observed at approximately the same potential of the mediator species (Epa = 414 mV).

The electrocatalytic response was confirmed by performing a blank test, in the absence of the ferrocenecarboxylic acid, i.e., using CCE (instead of modified CCE) as electrode material. In this case, the increase in the anodic peak was considerably lower, even in the presence of 10 × 10−3 mol L−1 of DA and slightly dislocated to higher positive potential (Epa = 448 V). The plot of peak current against the square root of the scan rate in the range of 10–100 mV s−1 showed a linear relation (with r = 0.996), indicating a diffusion controlled process, what is expected for catalytic systems and it is advantageous for quantitative measurements. Moreover, in order to confirm that DA oxidation occurred by an electrocatalytical process when the CCE/Fc was employed, the dependence of the parameter Ipa/ν1/2 on scan rate was plotted, as shown in Figure 5. According to Nicholson and Shain [34] a non-linear relationship of the plot Ipa/ν1/2 vs. ν exhibit the characteristic shape of a typical EC (electrochemical-chemical) catalytic process, as observed for the CCE modified with ferrocenecarboxylic acid. These results are similar to those obtained for other electrocatalytical systems for DA oxidation [27].

The redox process of DA also involves protons, hence the pH of the electrolyte is an important parameter which influences its oxidation [35]. From Figure 6A, it can be observed that the current is not significantly influenced by the pH differences, however the peak potential is shifted considerably to high positive potentials as the pH decreases (Figure 6B). The pKa for DA molecule is 8.9, which means that at the studied pH range, the reduced form of dopamine (cationic form) is predominant [36]. Therefore, as the pH decreases, the DA oxidation reaction becomes more difficult, which causes the shift of Epa to more positive potentials values, as can be observed in Figure 6B.

The pH chosen for further measurements was 7.0, since that in this condition Epa is not dislocated much to higher positive potentials and it is close to the physiological pH, what favors the application of the modified electrode in living systems.

In order to obtain the analytical parameters for the modified CCE, square wave voltammetry (SWV) experiments were carried out due to its higher sensibility when compared to the cyclic voltammetry technique, at several DA concentrations in 0.1 molL−1 phosphate buffer (pH 7.0). Firstly, the SWV parameters were optimized to obtain the best conditions for DA determination. The effect of frequency changes on the peak current was studied between the range of 10–100 s−1 and the results showed that the frequencies up to 60 s−1 cause an increase in peak current. However, at higher frequencies the currents decrease considerably. Therefore, f = 60 s−1 was chosen as optimum frequency value. The other effect studied was pulse amplitude in the range of 10–100 mV (with 10 mV intervals). The results obtained showed that, increasing the pulse heights up to 60 mV, an increase in peak current will be caused. Pulse heights higher than 60 mV causes broadening of the CCE/Fc voltammogram and the decreasing of the analyte peak current intensity. The effect of scan increment, which determines the amount of potential changes between two data points in the experiment, was also investigated. Scan increment values in the range of 1.0–10 mV (with 1 mV intervals) were applied to the electrodes and the corresponding voltammograms were recorded. The resulting peak currents showed that, by increasing the scan increment up to 2.0 mV values, the voltammograms’ peak current will also increase steadily. Therefore, the best conditions encountered were: f = 60 s−1 a = 60 mV e ΔE = 2.0 mV. Besides the higher sensitivity of the technique SWV, it was observed a shift of oxidation peak potentials to more negative regions (approximately Ep = 237 mV). This shift is described by Souza et al. as a result of scan rate. An explanation for this behavior is that, when high scan rates are applied, the influence of the increment (ΔE) is increased and can cause displacement in values of peak potentials [37]. Using these optimized conditions, the proposed sensor has shown a linear response range from 1.0 to 2.5 μmol L−1 (Figure 7), which can be expressed according to the following equation: Ip (μA) = −6.31 + 1.47 × 107 [DA/mol L−1] (R = 0.997). A detection limit of 0.45 μmol L−1 was determined using a 3σ/slope ratio, where σ is the standard deviation calculated from the ten background current values (blank measurements), determined according to IUPAC recommendations [38].

When compared to the non-modified CCE, the CCE/Fc has showed a lower detection limit and higher sensitivity for DA determination, as can be seen in Figure 8. This behavior can probably be related to the affinity between the electrocatalyst (ferrocenecarboxylic acid) and the analyte (DA), which consequently enhances the efficiency of the electron transfer processes. Ferrocene derived compounds have been used as electron mediators in modification of carbon paste electrodes [39], glassy carbon [40] and also in the preparation of films on gold surfaces for determination of DA [41].

The influence of ascorbic acid (AA) in the electrochemical oxidation of DA was evaluated using the SWV technique. According to Figure 9, using this technique the CCE/Fc presented only one peak which appeared at 230 mV. After DA addition, a considerable current peak increase is observed at the same potential. In contrast, in the presence of AA, a peak appeared at 50 mV, probably due to the AA oxidation at the CCE/Fc. Therefore, different from described in the literature, the current peak did not increase with AA concentration until 1.0 mmol L−1 [42]. This behavior can be explained considering that as the pKa of ferrocenecarboxylic is 4.2, in the electrolyte solution at pH 7.0, the anionic form of AA is predominant and it is responsible for the electrochemical response. At these conditions the CCE/Fc probably repels the AA species, hindering their approximation to the electrode surface, and causing a decrease of the electrode sensitivity for these species compared to the DA molecules (which is in the cationic form at pH 7.0).

When DA and AA were added simultaneously in the electrochemical cell, two peaks were observed, one at 50 mV and another at 250 mV related to AA and DA, respectively. These results indicated that, using the CCE/Fc it is possible to detect DA without AA interference, since the AA oxidizes at a different potential from DA (with a peak separation of approximately 200 mV) and the AA potential does not increase linearly with concentration.

The analytical parameters obtained are comparable with results reported in the literature for determination of DA and AA at the surface different composite electrodes (Table 1), although its linear response range is considerably narrow and should be improved.

4. Conclusions

In this paper, we have presented a carbon ceramic electrode prepared by the sol–gel technique and modified with ferrocenecarboxylic acid. SEM images coupled to surface element mapping (EDS) indicated that the components are homogeneously dispersed in the sample but with some agglomeration of ferrocene species. The electrochemical results demonstrated that the CCE/Fc is capable of determining dopamine with good sensitivity and selectivity compared to other similar carbon-based electrodes. Moreover, square wave voltammetric experiments realized in the presence of both AA and DA showed two peaks one at 50 mV and another at 250 mV (related to AA and DA, respectively). These results indicated that using the CCE/Fc it is possible to detect DA without AA interference, since the AA oxidizes at a different potential of DA (with a peak separation of approximately 200 V) and the AA potential does not increase linearly with concentration. CCE/Fc is a robust and stable material which is likely to be used on the construction of electrochemical sensors, also coupled with enzymes, which can be easily incorporated in the matrix surface.

Acknowledgments

The authors are grateful to Adilson Luiz Chinelatto for SEM and EDS measurements and CNPQ and Fundação Araucária for the financial assistance.

References and Notes

  1. Thenmozhi, K.; Narayanan, S.S. Amperometric hydrogen peroxide sensor based on a sol-gel-derived ceramic carbon composite electrode with toluidine blue covalently immobilized using 3-aminopropyltrimethoxysilane. Anal. Bioanal. Chem 2007, 387, 1075–1082. [Google Scholar]
  2. Rozniecka, E.; Shul, G.; Sirieix-Plenet, J.; Gaillon, L.; Opallo, M. Electroactive ceramic carbon electrode modified with ionic liquid. Electrochem. Commun 2005, 7, 299–304. [Google Scholar]
  3. Sheng, Q.; Yu, H.; Zheng, J. Solid state electrochemical of the erbium hexacyanoferrate-modified carbon ceramic electrode and its electrocatalytic oxidation of L-cysteine. J. Solid State Electrochem 2008, 12, 1077–1084. [Google Scholar]
  4. Salimi, A.; Miranzadeh, L.; Hallaj, R. Amperometric and voltammetric detection of hydrazine using glassy carbon electrodes modified with carbon nanotubes and catechol derivatives. Talanta 2007, 70, 823–832. [Google Scholar]
  5. Razmi, H.; Habibi, E. Nanomolar detection of hydrogen peroxide at a new polynuclear cluster of tin pentacyanonitrosylferrate nanoparticle-modified carbon ceramic electrode. Anal. Biochem 2009, 392, 126–132. [Google Scholar]
  6. Tsionsky, M.; Gun, G.; Giezer, V.; Lev, O. Sol-gel-derived ceramic-carbon composite electrodes—Introduction and scope of applications. Anal. Chem 1994, 66, 1747–1753. [Google Scholar]
  7. Lev, O.; Wu, Z.; Bharathi, S.; Glezer, V.; Modestov, A.; Gun, J.; Rabinovich, L.; Sampath, S. Sol-gel materials in electrochemistry. Chem. Mater 1997, 9, 2354–2375. [Google Scholar]
  8. Alfaya, A.S.; Kubota, L.T. Utilização de Materiais Obtidos pelo Processo de Sol-Gel na Construção de Biossensores. Quim. Nova 2002, 25, 835–841. [Google Scholar]
  9. Rabinovich, L.; Lev, O. Sol-gel derived composite ceramic carbon electrodes. Electroanalysis 2001, 4, 265–275. [Google Scholar]
  10. Zou, H.; Wu, S.S.; Shen, J. Polymer/silica nanocomposites: Preparation, characterization, properties, and applications. Chem. Rev 2008, 108, 3893–3957. [Google Scholar]
  11. Tsionsky, M.; Lev, O. Electrochemical Composite Carbon—Ceramic Gas Sensors: Introduction and Oxygen Sensing. Anal. Chem 1995, 67, 2409–2414. [Google Scholar]
  12. Sun, D.; Zhu, L.; Zhu, G. Glassy carbon ceramic composite electrodes. Anal. Chim. Acta 2007, 564, 243–247. [Google Scholar]
  13. Chen, H.; Dong, S. Direct electrochemistry and electrocatalysis of horseradish peroxidase immobilized in sol–gel-derived ceramic–carbon nanotube nanocomposite film. Biosens. Bioelectron 2007, 22, 1811–1815. [Google Scholar]
  14. Zheng, J.; Sheng, Q.; Li, L.; Shen, Y. Bismuth hexacyanoferrate-modified carbon ceramic electrodes prepared by electrochemical deposition and its electrocatalytic activity towards oxidation of hydrazine. J. Electroanal. Chem 2007, 611, 155–166. [Google Scholar]
  15. Sheng, Q.; Yu, H.; Zheng, J. Hydrogen peroxide determination by carbon ceramic electrodes modified with pyrocatechol violet. Electrochim. Acta 2007, 52, 7300–7306. [Google Scholar]
  16. Arguello, J.; Magosso, H.A.; Ramos, R.R.; Canevari, T.C.; Landers, R.; Pimentel, V.L.; Gushikem, Y. Structural and electrochemical characterization of a cobalt phthalocyanine bulk-modified SiO2/SnO2 carbon ceramic electrode. Electrochim. Acta 2009, 54, 1948–1953. [Google Scholar]
  17. Leia, C.; Huc, S.; Gaoa, N.; Shena, G.; Yua, R. An amperometric hydrogen peroxide biosensor based on immobilizing horseradish peroxidase to a nano-Au monolayer supported by sol–gel derived carbon ceramic electrode. Bioelectrochemistry 2004, 65, 33–39. [Google Scholar]
  18. Arguello, J.; Leidens, V.L.; Magosso, H.A.; Ramos, R.R.; Gushikem, Y. Simultaneous voltammetric determination of ascorbic acid, dopamine and uric acid by methylene blue adsorbed on a phosphorylated zirconia–silica composite electrode. Electrochim. Acta 2008, 54, 560–565. [Google Scholar]
  19. Salimi, A.; Abdi, K. Enhancement of the analytical properties and catalytic activity of a nickel hexacyanoferrate modified carbon ceramic electrode prepared by two-step sol–gel technique: application to amperometric detection of hydrazine and hydroxyl amine. Talanta 2007, 63, 475–483. [Google Scholar]
  20. Tian, F.; Zhu, G. Bienzymatic amperometric biosensor for glucose based on polypyrrole/ceramic carbon as electrode material. Anal. Chim. Acta 2002, 451, 251–258. [Google Scholar]
  21. Pessoa, C.A.; Gushikem, Y.; Kubota, L.T. Ferrocenecarboxylic acid adsorbed on Nb2O5 film grafted on a SiO2 surface: NADH oxidation study. Electrochim. Acta 2001, 46, 2499–2505. [Google Scholar]
  22. Merchant, S.A.; Tran, T.O.; Meredith, M.T.; Cline, T.C.; Glatzhofer, D.T.; Schmidtke, D.W. High-sensitivity amperometric biosensors based on ferrocene-modified linear poly(ethylenimine). Langmuir 2009, 25, 7736–7742. [Google Scholar]
  23. Molina, A.; Soto, C.M.; Gonzalez, J. Electrocatalytic Responses at Mediator Modified Electrodes with Several Cyclic Step and Cyclic Sweep Potential Techniques. Application to the Oxidation of Ascorbate at a Ferrocene-Monolayer Modified Gold Electrode. Anal. Chem 2009, 81, 6830–6836. [Google Scholar]
  24. Ndamanisha, J.C.; Guo, L. Electrochemical determination of uric-acid at ordered mesoporous carbon functionalized with ferrocenecarboxylic acid-modified electrode. Biosens. Bioelectron 2008, 23, 1680–1685. [Google Scholar]
  25. Lafitte, V.G.H.; Wang, W.; Yashina, A.S.; Lawrence, N.S. Anthraquinone-ferrocene film electrodes: Utility in pH and oxygen sensing. Electrochem. Commun 2008, 10, 1831–1834. [Google Scholar]
  26. Fernandez, L.; Carrero, H. Electrochemical evaluation of ferrocene carboxylic acids confined on surfactant-clay modified glassy carbon electrodes: oxidation of ascorbic acid and uric acid. Electrochim. Acta 2005, 50, 1233–1240. [Google Scholar]
  27. Skeika, T.; Marcovicz, C.; Nakagaki, S.; Fujiwara, S.T.; Wohnrath, K.; Nagata, N.; Pessoa, C.A. Electrochemical studies of an iron porphyrin immobilized on Nb2O5/SiO2 and its application for simultaneous determination of dopamine and ascorbic acid using multivariate calibration methodology. Electroanalysis 2007, 19, 2543–2550. [Google Scholar]
  28. Ge, P.; Du, Y.; Xu, J.; Chen, H. Detection of dopamine based on the unique property of gold nanofilm. J. Electroanal. Chem 2009, 633, 182–186. [Google Scholar]
  29. Tashkhourian, J.; Nezhad, M.R.H.; Khodavesi, J.; Javadi, S. Silver nanoparticles modified carbon nanotube paste electrode for simultaneous determination of dopamine and ascorbic acid. J. Electroanal. Chem 2009, 633, 85–91. [Google Scholar]
  30. Salimi, A.; MamKhezri, H.; Hallaj, R. Simultaneous determination of ascorbic acid, uric acid and neurotransmitters with a carbon ceramic electrode prepared by sol–gel technique. Talanta 2006, 70, 823–832. [Google Scholar]
  31. Ensafi, A.A.; Taei, M.; Khayamian, T.A. differential pulse voltammetric method for simultaneous determination of ascorbic acid, dopamine, and uric acid using poly (3-(5-chloro-2-hydroxyphenylazo)-4,5-dihydroxynaphthalene-2,7-disulfonic acid) film modified glassy carbon electrode. J. Electroanal. Chem 2009, 633, 212–220. [Google Scholar]
  32. Bard, A.J.; Faulkner, L.R. Electrochemical Methods, Fundamentals and Applications; Wiley: New York, NY, USA, 1980. [Google Scholar]
  33. De Santis, G.; Fabbrizzi, L.; Licchelli, M.; Pallavicini, P. Controlling the acidity of the carboxílic group by a ferrocene based redox switch. Inorg. Chim. Acta 1994, 225, 239–244. [Google Scholar]
  34. Nicholson, R.S.; Shain, I. Theory of stationary electrode polarography: single scan and cyclic methods applied to reversible, irreversible, and kinetic systems. Anal. Chem 1964, 36, 706–723. [Google Scholar]
  35. Nasri, Z.; Shams, E. Application of silica gel as an effective modifier for the voltammetric determination of dopamine in the presence of ascorbic acid and uric acid. Electrochim. Acta 2009, 54, 7416–7421. [Google Scholar]
  36. Wang, H.; Wang, L.J.; Shi, Z.F.; Guo, Y.; Cao, X.P.; Zhang, H.L. Application of self-assembled “molecular wires” monolayers for electroanalysis of dopamine. Electrochem. Commun 2006, 8, 1779–1783. [Google Scholar]
  37. Analytical Methods Committee Recomendations for definition, estimation and use of the detection limit. Analyst 1987, 112, 199–204.
  38. Souza, D.; Galli, A.; Calegaro, M.L.; Machado, S.A.S.; Pires, R.C. Utilização da Múltipla Voltametria de Onda Quadrada na Determinação Eletroanalítica de Compostos Orgânicos e Inorgânicos. Quím. Nova 2007, 30, 458–463. [Google Scholar]
  39. Kamyabi, M.A.; Aghajanloo, F. Electrocatalytic Response of Dopamine at a Carbon Paste Electrode Modified with Ferrocene. Croat. Chem. Acta 2009, 82, 599–606. [Google Scholar]
  40. Jiao, S.; Li, M.; Wang, C.; Chen, D.; Fang, B. Fabrication of Fc-SWNTs modified glassy carbon electrode for selective and sensitive determination of dopamine in the presence of AA and UA. Electrochim. Acta 2007, 52, 5939–5944. [Google Scholar]
  41. Liu, A.H.; Anzai, J. Ferrocene-containing polyelectrolyte multilayer film-covered electrodes: Electrocatalytic determination of ascorbic acid and use of inner blocking layers to improve the upper detection limit of the electrodes. Electrochim. Acta 2005, 50, 1233–1240. [Google Scholar]
  42. Pournaghi-Azar, M.H.; Ojani, R. Catalytic oxidation of ascorbic acid by some ferrocene derivative mediators at the glassy carbon electrode. Application to the voltammetric resolution of ascorbic acid and dopamine in the same sample. Talanta 1995, 42, 1839–1848. [Google Scholar]
  43. Abbaspour, A.; Ghaffarinejad, A. Method for Preparation of a Sol-Gel-Derived Carbon Ceramic Electrode Using Microwave Irradiation. Anal. Chem 2009, 81, 3660–3664. [Google Scholar]
  44. Pandey, P.C.; Upadhyay, S.; Tiwari, I.; Singh, G.; Tripathi, V.S. A novel ferrocene encapsulated palladium-linked ormosil-based electrocatalytic dopamine biosensor. Sens. Actuat. B 2001, 75, 48–55. [Google Scholar]
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Figure 1. EDS images for Fe mapping (A) 500 X and (B) 1,500 X and the corresponding (C) EDS analysis.
Figure 1. EDS images for Fe mapping (A) 500 X and (B) 1,500 X and the corresponding (C) EDS analysis.
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Figure 2. Cyclic voltammograms of the CCE/Fc with different amounts of ferrocene: 0.0025 g; 0.005 g and 0.010 g (ν = 50 mV s−1 and [NaCl] = 0.5 mol L−1).
Figure 2. Cyclic voltammograms of the CCE/Fc with different amounts of ferrocene: 0.0025 g; 0.005 g and 0.010 g (ν = 50 mV s−1 and [NaCl] = 0.5 mol L−1).
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Figura 3. Cyclic voltammograms of the CCE/Fc at different scan rates (10 to 100 mV s−1). Inserted figure: Relationship between Ipa versus ν. Supporting electrolyte: [NaCl] = 0.5 mol L−1.
Figura 3. Cyclic voltammograms of the CCE/Fc at different scan rates (10 to 100 mV s−1). Inserted figure: Relationship between Ipa versus ν. Supporting electrolyte: [NaCl] = 0.5 mol L−1.
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Figure 4. CCE/Fc stability after repeated 100 cycles. Supporting electrolyte: [NaCl] = 0.5 mol L−1.
Figure 4. CCE/Fc stability after repeated 100 cycles. Supporting electrolyte: [NaCl] = 0.5 mol L−1.
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Figure 5. Plot of Ipa/ν1/2 against scan rate for CCE/ferrocene in presence of [DA] = 1.0 × 10−3 mol L−1. Supporting electrolyte: phosphate buffer (0.1 mol L−1) pH = 7.0.
Figure 5. Plot of Ipa/ν1/2 against scan rate for CCE/ferrocene in presence of [DA] = 1.0 × 10−3 mol L−1. Supporting electrolyte: phosphate buffer (0.1 mol L−1) pH = 7.0.
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Figure 6. Effect of pH of the supporting electrolyte NaCl 0.5 mol L−1 on the Ipa values (A), and Epa values (B) of the CCE/Fc in presence of [DA] = 1.0 mmol L−1.
Figure 6. Effect of pH of the supporting electrolyte NaCl 0.5 mol L−1 on the Ipa values (A), and Epa values (B) of the CCE/Fc in presence of [DA] = 1.0 mmol L−1.
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Figure 7. Square wave voltammograms of CCE/Fc at different DA concentrations in the range of 1.0 a 2.5 μmol L−1 in 0.1 mol L−1 phosphate buffer at pH 7.0. SWV Parameters: f = 60 s−1, a = 60 mV e ΔE = 2.0 mV.
Figure 7. Square wave voltammograms of CCE/Fc at different DA concentrations in the range of 1.0 a 2.5 μmol L−1 in 0.1 mol L−1 phosphate buffer at pH 7.0. SWV Parameters: f = 60 s−1, a = 60 mV e ΔE = 2.0 mV.
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Figure 8. (A) SWV of CCE/Fc and CCE in absence and presence [DA] = 2.5 μmol L−1 in 0.1 mol L−1 phosphate buffer at pH 7.0. (B) Analytical curves obtained with CCE/Fc and CCE at different DA concentrations in the range of 1.0 a 2.5 μmol L−1 in 0.1 mol L−1 phosphate buffer at pH 7.0. SWV Parameters: f = 60 s−1, a = 60 mV e ΔE = 2.0 mV.
Figure 8. (A) SWV of CCE/Fc and CCE in absence and presence [DA] = 2.5 μmol L−1 in 0.1 mol L−1 phosphate buffer at pH 7.0. (B) Analytical curves obtained with CCE/Fc and CCE at different DA concentrations in the range of 1.0 a 2.5 μmol L−1 in 0.1 mol L−1 phosphate buffer at pH 7.0. SWV Parameters: f = 60 s−1, a = 60 mV e ΔE = 2.0 mV.
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Figure 9. SWV of CCE/Fc in absence and presence of AA and DA, both in a concentration of 2.5 μmol L−1 in 0.1 mol L−1 phosphate buffer at pH 7.0. [AA] = [DA] simultaneous additions in same concentrations. SWV Parameters: f = 60 s−1, a = 60 mV e ΔE = 2.0 mV.
Figure 9. SWV of CCE/Fc in absence and presence of AA and DA, both in a concentration of 2.5 μmol L−1 in 0.1 mol L−1 phosphate buffer at pH 7.0. [AA] = [DA] simultaneous additions in same concentrations. SWV Parameters: f = 60 s−1, a = 60 mV e ΔE = 2.0 mV.
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Table
Table 1. Analytical parameters for carbon composite electrodes for detection of DA and AA.
Table 1. Analytical parameters for carbon composite electrodes for detection of DA and AA.
ElectrodeMethodAnalytesLinear range (μmol L−1)Limit of detection (μmol L−1)Sensitivity (μA/μmol L−1)Ref.
Carbon ceramic compositeSWVDA0.5–200.10.7530
AA0.10.754
Methylene blue- zirconia-silica mixed oxideDPVDA in presence of AA40–1604.00.4718
MWCCE *Cyclic voltammetricDA4–1,0001.50.1343
Ferrocene-Pd-ormosilAmperometryDA1,000–8,000500.1344
CCE/FerroceneSWVDA1–2.50.4514.7This work
AA——

*sol-gel carbon ceramic electrode (CCE) prepared by microwave (MW) irradiation

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Skeika, T.; Zuconelli, C.R.; Fujiwara, S.T.; Pessoa, C.A. Preparation and Electrochemical Characterization of a Carbon Ceramic Electrode Modified with Ferrocenecarboxylic Acid. Sensors 2011, 11, 1361-1374. https://doi.org/10.3390/s110201361

AMA Style

Skeika T, Zuconelli CR, Fujiwara ST, Pessoa CA. Preparation and Electrochemical Characterization of a Carbon Ceramic Electrode Modified with Ferrocenecarboxylic Acid. Sensors. 2011; 11(2):1361-1374. https://doi.org/10.3390/s110201361

Chicago/Turabian Style

Skeika, Tatiane, Cristiane R. Zuconelli, Sergio T. Fujiwara, and Christiana A. Pessoa. 2011. "Preparation and Electrochemical Characterization of a Carbon Ceramic Electrode Modified with Ferrocenecarboxylic Acid" Sensors 11, no. 2: 1361-1374. https://doi.org/10.3390/s110201361

APA Style

Skeika, T., Zuconelli, C. R., Fujiwara, S. T., & Pessoa, C. A. (2011). Preparation and Electrochemical Characterization of a Carbon Ceramic Electrode Modified with Ferrocenecarboxylic Acid. Sensors, 11(2), 1361-1374. https://doi.org/10.3390/s110201361

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