Doxycycline Attenuates Cancer Cell Growth by Suppressing NLRP3-Mediated Inflammation
Abstract
:1. Introduction
2. Results
2.1. Doxycycline Attenuates NLRP3 Inflammasome Activation
2.2. Doxycycline Decreased Cell Proliferation and Viability
2.3. The Effect of Doxycycline on Cytokine Secretion
3. Discussion
4. Materials and Methods
4.1. Cell Lines and Maintenance
4.2. Reagents
4.3. Real Time-qPCR
4.4. Western Blot
4.5. Enzyme-Linked Immunosorbent Assay (ELISA)
4.6. Real-Time Cell Proliferation Assay
4.7. Annexin V Assay
4.8. Cytokine Assay
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Alsaadi, M.; Tezcan, G.; Garanina, E.E.; Hamza, S.; McIntyre, A.; Rizvanov, A.A.; Khaiboullina, S.F. Doxycycline Attenuates Cancer Cell Growth by Suppressing NLRP3-Mediated Inflammation. Pharmaceuticals 2021, 14, 852. https://doi.org/10.3390/ph14090852
Alsaadi M, Tezcan G, Garanina EE, Hamza S, McIntyre A, Rizvanov AA, Khaiboullina SF. Doxycycline Attenuates Cancer Cell Growth by Suppressing NLRP3-Mediated Inflammation. Pharmaceuticals. 2021; 14(9):852. https://doi.org/10.3390/ph14090852
Chicago/Turabian StyleAlsaadi, Mohammad, Gulcin Tezcan, Ekaterina E. Garanina, Shaimaa Hamza, Alan McIntyre, Albert A. Rizvanov, and Svetlana F. Khaiboullina. 2021. "Doxycycline Attenuates Cancer Cell Growth by Suppressing NLRP3-Mediated Inflammation" Pharmaceuticals 14, no. 9: 852. https://doi.org/10.3390/ph14090852
APA StyleAlsaadi, M., Tezcan, G., Garanina, E. E., Hamza, S., McIntyre, A., Rizvanov, A. A., & Khaiboullina, S. F. (2021). Doxycycline Attenuates Cancer Cell Growth by Suppressing NLRP3-Mediated Inflammation. Pharmaceuticals, 14(9), 852. https://doi.org/10.3390/ph14090852