Association Study of Genes Associated to Asthma in a Specific Environment, in an Asthma Familial Collection Located in a Rural Area Influenced by Different Industries
Abstract
:1. Introduction
2. Methods
2.1. Subjects
2.2. Gene Selection and Genotypes
All samples (n = 1,428) | Probands a (n = 254) | Family members (n = 1,174) | Exposed b | ||
---|---|---|---|---|---|
Children (na = 327) (np = 194) | Family members (na = 729)(np = 431) | ||||
General descriptive data | |||||
M:F ratio | 1:1.2 | 1:1.2 | 1:1.2 | 1:1.2 | 1:1.1 |
1:1.4 | 1:1.2 | ||||
Age, mean (range) c | 39 (2–93) | 18 (3–62) | 43 (2–93) | 22 (2–71) | 47 (3–93) |
25 (2–75) | 47 (3–88) | ||||
Age median | 39 | 16 | 43 | 17 | 45 |
19 | 45 | ||||
Smoking status, n (%) d | |||||
non smoker | 721 (51) | 208 (84) | 512 (44) | 239 (74) | 279 (39) |
139 (74) | 163 (39) | ||||
ex smoker | 405 (29) | 14 (6) | 391 (34) | 38(12) | 277 (39) |
22 (12) | 157 (37) | ||||
smoker | 283 (20) | 27 (11) | 256 (22) | 47 (14) | 162 (22) |
27 (14) | 103 (24) | ||||
Clinical descriptive data | |||||
FEV1, % pred. (SD) e | 94 (42) | 93 (34) | 95 (43) | 93 (18) | 96 (18) |
91(16) | 95 (18) | ||||
PC20, mg/mL (SD) f | 7.76 (5.21) | 2.50 (3.61) | 10.06 (5.04) | 6.40 (10.85) | 27.83 (26.4) |
7.60 (13.02) | 29.30 (26.98) | ||||
IgE, μg/L (SD) g | 135 (5) | 236 (5) | 118 (4) | 693 (1738) | 260 (517.1) |
891 (3063.9) | 394 (1975.6) | ||||
Asthma, n (%) h | 693 (49) | 253 (100) | 440 (38) | 327 (100) | 192 (27) |
194 (100) | 116 (27) |
2.3. Environment Characteristics and Air Pollution Data
Air pollutant | Years | Mean annual value (ppb) | Period (hour) a | Median (ppb) | Maximum (ppb) |
---|---|---|---|---|---|
Ozone | 1997 to 2000 | 27.7 | 1 | 28 | 76.2 |
8 | 27.8 | 72.2 | |||
Sulphur dioxide | 9.41 | 1 | 1.6 | 150 | |
24 | 3.7 | 82.4 |
2.4. Statistical Analysis
3. Results
Industry | Gene symbol/position | SNP/position a | Allele b | T | U | OR | CHISQ | p value a | p value with permutations | p value threshold (Meff) c |
---|---|---|---|---|---|---|---|---|---|---|
All samples | CAT/11p13 | rs11032703/intron 1 | T/C | 90 | 52 | 1.731 | 10.17 | 0.001428 | 0.0029 | 0.00474 |
rs2300181/intron 6 | T/C | 90 | 120 | 0.75 | 4.286 | 0.03843 | 0.04225 | (10.5501) | ||
rs511895/intron 10 | T/C | 170 | 129 | 1.318 | 5.622 | 0.0177 | 0.0227 | |||
NQO1/16q22.1 | rs1800566/exon 5 (Pro187Ser) | A/G | 86 | 118 | 0.7288 | 5.02 | 0.02506 | 0.03135 | 0.01686 | |
(2.9652) | ||||||||||
rs1437135/intron 5 | G/A | 86 | 118 | 0.7288 | 5.02 | 0.02506 | 0.03135 | |||
Aluminium | CAT/11p13 | rs11032703/intron 1 | T/C | 71 | 41 | 1.732 | 8.036 | 0.004586 | 0.0055 | 0.00442 |
(11.3167) | ||||||||||
NQO1/16q22.1 | rs1800566/exon 5 (Pro187Ser) | A/G | 63 | 90 | 0.7 | 4.765 | 0.02905 | 0.03595 | 0.01827 | |
(2.7369) | ||||||||||
rs1437135/intron 5 | G/A | 63 | 90 | 0.7 | 4.765 | 0.02905 | 0.03595 | |||
Pulp and Paper | GSTP1/11q13 | rs1695/exon 5 (Ile105Val) | G/A | 46 | 70 | 0.6571 | 4.966 | 0.02586 | 0.0354 | 0.02072 |
(2.4134) |
Industry | Gene symbol/position | Locus | Haplotype | T | U | CHISQ | p value a | p value Threshold b(Meff) |
---|---|---|---|---|---|---|---|---|
All samples | CAT/11p13 | H1 | rs208679A rs7118388G rs7944397A rs208682C rs554518C | 162.5 | 123.8 | 5.223 | 0.0223 | 0.00474 (10.5509) |
H1 | rs208679A rs7118388A rs7944397A rs208682C rs554518C | 113.5 | 148.9 | 4.779 | 0.0288 | |||
H2 | rs1001179C rs480575A rs2284369A rs11032703T rs769218G rs2300181C rs7933285C rs511895T rs10488736C | 91 | 54.4 | 9.221 | 0.002406 | |||
EPHX1/1q42.1 | H3 | rs4653695A rs2740174A rs360063A rs1009668C | 150.8 | 114.9 | 4.842 | 0.02777 | 0.00352 (14.2206) | |
NQO1/16q22.1 | H1 | rs1800566A rs4986998G rs689452G rs1437135G rs689457C rs2917682T | 86 | 119 | 5.312 | 0.02118 | 0.01686 (2.9652) | |
Aluminium | CAT/11p13 | H2 | rs1001179C rs480575A rs2284369A rs11032703T rs769218G rs2300181C rs7933285C rs511895T rs10488736C | 71 | 42 | 7.442 | 0.00637 | 0.00442 (11.3167) |
NQO1/16q22.1 | H1 | rs1800566A rs4986998G rs689452G rs1437135G rs689457C rs2917682T | 63 | 91 | 5.091 | 0.02405 | 0.01827 (2.7369) | |
Pulp and Paper | EPHX1/1q42.1 | H4 | rs360063A rs1009668C | 62 | 41 | 4.282 | 0.03853 | 0.00311 |
GSTP1/ 11q13 | H1 | rs1695A rs1138272C | 69 | 45 | 5.053 | 0.02459 | 0.02072 (2.4134) |
4. Discussion
5. Conclusions
Acknowledgments
Conflict of Interest
References
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Morin, A.; Brook, J.R.; Duchaine, C.; Laprise, C. Association Study of Genes Associated to Asthma in a Specific Environment, in an Asthma Familial Collection Located in a Rural Area Influenced by Different Industries. Int. J. Environ. Res. Public Health 2012, 9, 2620-2635. https://doi.org/10.3390/ijerph9082620
Morin A, Brook JR, Duchaine C, Laprise C. Association Study of Genes Associated to Asthma in a Specific Environment, in an Asthma Familial Collection Located in a Rural Area Influenced by Different Industries. International Journal of Environmental Research and Public Health. 2012; 9(8):2620-2635. https://doi.org/10.3390/ijerph9082620
Chicago/Turabian StyleMorin, Andréanne, Jeffrey R. Brook, Caroline Duchaine, and Catherine Laprise. 2012. "Association Study of Genes Associated to Asthma in a Specific Environment, in an Asthma Familial Collection Located in a Rural Area Influenced by Different Industries" International Journal of Environmental Research and Public Health 9, no. 8: 2620-2635. https://doi.org/10.3390/ijerph9082620
APA StyleMorin, A., Brook, J. R., Duchaine, C., & Laprise, C. (2012). Association Study of Genes Associated to Asthma in a Specific Environment, in an Asthma Familial Collection Located in a Rural Area Influenced by Different Industries. International Journal of Environmental Research and Public Health, 9(8), 2620-2635. https://doi.org/10.3390/ijerph9082620