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Article

Incidence and Risk Factors for Development of Cardiac Toxicity in Adult Patients with Newly Diagnosed Acute Myeloid Leukemia

by
Blanca Boluda
1,2,*,
Antonio Solana-Altabella
2,3,
Isabel Cano
1,2,
David Martínez-Cuadrón
1,2,4,
Evelyn Acuña-Cruz
1,2,
Laura Torres-Miñana
1,2,
Rebeca Rodríguez-Veiga
1,2,
Irene Navarro-Vicente
1,2,
David Martínez-Campuzano
1,
Raquel García-Ruiz
1,
Pilar Lloret
1,
Pedro Asensi
1,
Ana Osa-Sáez
5,
Jaume Aguero
5,
María Rodríguez-Serrano
5,
Francisco Buendía-Fuentes
5,
Juan Eduardo Megías-Vericat
2,
Beatriz Martín-Herreros
1,2,
Eva Barragán
1,2,4,
Claudia Sargas
1,2,
Maribel Salas
6,7,
Margaret Wooddell
6,
Charles Dharmani
6,
Miguel A. Sanz
2,4,
Javier De la Rubia
1,2,4,8 and
Pau Montesinos
1,2,4add Show full author list remove Hide full author list
1
Hematology Department, Hospital Universitari i Politècnic La Fe, 46026 Valencia, Spain
2
Instituto de Investigación Sanitaria La Fe, 46026 Valencia, Spain
3
Pharmacy Department, Hospital Universitari i Politècnic La Fe, 46026 Valencia, Spain
4
CIBERONC, Instituto de Salud Carlos III, 28029 Madrid, Spain
5
Cardiology Department, Hospital Universitari i Politècnic La Fe, 46026 Valencia, Spain
6
Daiichi Sankyo, Inc., Basking Ridge, NJ 07920, USA
7
Center for Real-World Effectiveness and Safety of Therapeutics (CREST), University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA
8
Internal Medicine, School of Medicine and Dentistry, Catholic University of Valencia, 46001 Valencia, Spain
*
Author to whom correspondence should be addressed.
Cancers 2023, 15(8), 2267; https://doi.org/10.3390/cancers15082267
Submission received: 15 February 2023 / Revised: 3 April 2023 / Accepted: 6 April 2023 / Published: 12 April 2023
(This article belongs to the Collection Acute Myeloid Leukemia (AML))
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Abstract

:

Simple Summary

The incidence of cardiac morbimortality in acute myeloid leukemia (AML) is not well known. We aim to estimate the cumulative incidence (CI) of cardiac events in AML patients and to identify risk factors for their occurrence. We observed a high incidence of cardiac events (58.5%) among 525 treated patients, coupled with significant mortality due to cardiotoxicity (3.6%). The presence of relevant cardiac antecedents was the main risk factor for developing fatal cardiac events (hazard ratio (HR) = 1.9). Age ≥ 65 (HR = 2.2), relevant cardiac antecedents (HR = 1.4), and non-intensive chemotherapy (HR = 1.8) were associated with non-fatal cardiac events. We observed that, among 285 intensive therapy patients, median overall survival was decreased in those experiencing grade 3–4 cardiac events (p < 0.001). We identified prognostic factors that increase the risk of cardiac events, which may be useful in selecting high-risk patients for stringent cardiac monitoring and management.

Abstract

The incidence of cardiac morbimortality in acute myeloid leukemia (AML) is not well known. We aim to estimate the cumulative incidence (CI) of cardiac events in AML patients and to identify risk factors for their occurrence. Among 571 newly diagnosed AML patients, 26 (4.6%) developed fatal cardiac events, and among 525 treated patients, 19 (3.6%) experienced fatal cardiac events (CI: 2% at 6 months; 6.7% at 9 years). Prior heart disease was associated with the development of fatal cardiac events (hazard ratio (HR) = 6.9). The CI of non-fatal cardiac events was 43.7% at 6 months and 56.9% at 9 years. Age ≥ 65 (HR = 2.2), relevant cardiac antecedents (HR = 1.4), and non-intensive chemotherapy (HR = 1.8) were associated with non-fatal cardiac events. The 9-year CI of grade 1–2 QTcF prolongation was 11.2%, grade 3 was 2.7%, and no patient had grade 4–5 events. The 9-year CI of grade 1–2 cardiac failure was 1.3%, grade 3–4 was 15%, and grade 5 was 2.1%; of grade 1–2, arrhythmia was 1.9%, grade 3–4 was 9.1%, and grade 5 was 1%. Among 285 intensive therapy patients, median overall survival decreased in those experiencing grade 3–4 cardiac events (p < 0.001). We observed a high incidence of cardiac toxicity associated with significant mortality in AML.

1. Introduction

Despite the progress in modern chemotherapy achieved in recent decades, the prognosis of acute myeloid leukemia (AML) remains discouraging, with a considerable proportion of patients suffering induction failure or relapse after attaining complete remission (CR) [1,2]. AML patients receive potentially cardiotoxic drugs such as anthracyclines [3,4,5,6,7] and drugs with corrected QT interval (QTc) prolongations, such as FLT3 inhibitors [8,9,10,11] and azoles, among others [12,13]. Cardiotoxicity is known to occur among all cancer survivors; the United States (US) National Health and Nutrition Examination Survey, performed on 1807 cancer survivors and followed up for seven years, showed that up to 33% of the patients died of heart disease [14]. Nevertheless, there is still no ’standard‘ definition of cardiotoxicity [4,15], and the incidence of cardiac morbidity and mortality in the AML population is not well known. In addition to the potential toxicity of chemotherapy, cardiac events can emerge in patients with previous cardiac diseases, due to other concomitant medications, or as a complication of AML itself [3,5,16]. So far, no previous studies have provided a holistic view of cardiac toxicities in a real-world series of AML patients.
The aim of this retrospective chart review study is to provide insights into the overall incidence and risk factors for the occurrence of cardiac events in a large series of unselected adult AML patients in a single Spanish institution (Hospital Universitari I Politècnic La Fe).

2. Methods and Patients

2.1. Study Design and Population

This was a single-center, non-interventional, retrospective, systematic chart review study. The study population included adult patients diagnosed with AML during the study period (from 1 January 2011 to 30 June 2020) at the Hospital Universitari i Politècnic La Fe (HULaFe). Patients diagnosed with acute promyelocytic leukemia (APL) were excluded. AML patients who were at least 18 years old at diagnosis (i.e., index date) and had charts available for exhaustive review and data collection were included. Patients who participated in any interventional clinical trial from the index date were not excluded (i.e., with FLT3 inhibitors).
The protocol was approved by the local Clinical Research Ethics Committee of HULaFe, and patients provided informed consent in accordance with the principles of the Declaration of Helsinki.
The study population was made up of all adult patients with AML who were diagnosed at the HULaFe (overall cohort). The first-line (1L) cohort included treated patients who were part of the overall cohort, but they differed from the overall cohort in their follow-up period; overall cohort patients were followed until the last follow-up recorded or death, while 1L patients were followed until relapse/refractory (R/R) or death date.

2.2. Study Objectives and Variables

The primary objective was to describe the overall incidence of fatal cardiac events in AML patients from the primary diagnosis date at the HULaFe until the last follow-up or death. The secondary objective was to describe the overall incidence of non-fatal cardiac events (including life-threatening ones) in AML patients from the primary diagnosis date until the last follow-up or death.
Other objectives included (1) establishing risk factors for the development of cardiac events in AML; (2) determining the overall incidence of cardiac events in all AML patients from the primary diagnosis date until the end of the follow-up or the first R/R date during the front-line period (1L cohort); (3) establishing risk factors for the development of cardiac events during the front-line period (1L cohort); and (4) describing response rates and long-term outcomes (overall survival (OS), event-free survival (EFS)) in patients with or without cardiac events from the index date until the last follow-up.
The following information was collected at baseline when available: (1) Patient and disease characteristics, including age, gender, cardiac and other co-morbidities; French–American–British (FAB) classification, World Health Organization (WHO) classification [17], Eastern Cooperative Oncology Group (ECOG) scale, de novo or secondary AML, bone marrow assessment (blasts percentage), extramedullary involvement, cytogenetic risk stratification, gene mutations (FLT3-ITD, FLT3-TKD, NPM1), peripheral blood parameters, date of initial diagnosis, baseline cardiologic medication, prior treatment with anthracyclines. (2) Therapy-related variables: treatment regimen for AML; hematopoietic stem cell transplantation (HSCT) and date if performed; type of HSCT; investigational therapy in the first-line; FLT3 inhibitors; response assessment (complete remission (CR), complete remission with incomplete hematologic recovery (Cri), partial remission (PR), morphologic leukemia-free state (MLFS), disease progression/relapse, non-evaluable); date of response assessment; date of death; relapse; date of relapse; date of last follow-up. The following variables related to cardiac events were collected: date of cardiac event, cardiac event term (according to Common Terminology Criteria for Adverse Events Version 5.0 (CTCAE V5)) [18], grade, admission for an event (inpatient/outpatient, domiciliary hospitalization), level of care during hospitalization (ICU, hematology or cardiology), outcome of cardiac events (resolved/unresolved), concomitant medications, and causes contributing to cardiac events.
The systematic review of the patients’ medical records included the revision of all electrocardiograms (ECGs) available in the electronic clinical records by trained hematologists, with cardiologist consultation if needed; a systematic review of all clinical notes from diagnosis; and vital signs, focusing on heart rate and blood pressure. Deaths after a cardiac event were attributed to the cardiac event or to another cause (i.e., sepsis or AML progression) according to the investigator’s clinical judgment.

2.3. Definitions

The diagnosis date refers to the date of AML diagnosis. Refractory AML was defined as a failure to achieve CR/CRi after 1 or 2 courses of intensive-induction chemotherapy treatment [19,20], excluding patients with death in aplasia or death due to indeterminate cause. Patients treated with non-intensive chemotherapy approaches were considered refractory when they showed progressive disease, no clinical benefit, or a change in the treatment line. Relapse was defined as AML subjects who achieved a CR/CRi with prior line treatment and had a hematologic relapse. Treatments were classified as intensive chemotherapy (i.e., idarubicine and cytarabine (3 + 7); fludarabine–cytarabine-based (FLAG-based) or high- or intermediate-dose cytarabine-containing regimens) or non-intensive approaches (i.e., hypomethylating agents, low-dose cytarabine-based regimens (LDAC), FLT3 inhibitors as monotherapy).
Regarding cardiac antecedents, any prior history of cardiac disease was recorded, including arterial hypertension. We defined a category of clinically relevant cardiac antecedents including conditions with worse prognosis and severity: symptomatic heart failure, myocardial infarction, or ejection fraction (EF) < 50%. Several categories of cardiac events were analyzed: (1) myocardial ischemic events included the following (according to CTCAE): cardiac troponin increased, chest pain (cardiac), and myocardial infarction; (2) heart failure and related events included ejection fraction decreased, heart failure, left ventricular systolic dysfunction, pulmonary edema, and right ventricular dysfunction; (3) arrhythmia events included atrial fibrillation, atrial flutter, complete atrioventricular (AV) block, first degree AV block, Mobitz type II AV block, Mobitz type I AV block, paroxysmal atrial tachycardia, sick sinus syndrome, supraventricular tachycardia, torsade de pointes, ventricular arrhythmia, ventricular fibrillation, ventricular tachycardia, sudden death, cardiac arrest, and cardiorespiratory arrest. An isolated QTc prolongation was not considered in the category of arrhythmia events. The QTc interval was calculated using the Fridericia formula (QTcF). Echocardiograms for the ejection fraction were performed at diagnosis in patients treated with intensive chemotherapy and for unfit patients when clinically indicated or required by clinical trial; during follow-up, an echocardiogram was performed previous to stem cell transplant (both autologous and allogeneic) or when clinically indicated.
Life-threatening events were defined as those requiring an intensive care unit (ICU) admission or intensive management in a hematology unit as per clinical judgment.

2.4. Statistical Analyses

Descriptive statistics were calculated for all outcomes. Means or medians were provided for continuous variables and incidences and proportions for categorical variables. The crude incidence (rate or percentage) of patients developing a fatal cardiac event (number of fatal cardiac events/total number of patients for each study cohort) was calculated. Categorical variables were summarized by the number and proportion in each category. Chi-square with Yates’ correction, Mann–Whitney U, and Student’s t-tests were used to analyze differences in the distribution of variables among patient subsets. The proportion of subjects with missing data for key variables collected in the study was described in the tables as a separate category. Data collection methods (single-center) implemented in this study ensured that missing data were minimized.
For non-fatal and overall cardiac events, as patients could develop such complications several times during their observation period, the crude incidence (rate or percentage) was calculated accounting for the first cardiac event for each patient. For the univariate risk factor analyses (using the Fine and Gray method), we considered a significant correlation for p-values < 0.05. The cumulative incidence was also calculated (using the cumulative incidence method, as described by Fine and Gray) [21] and the first cardiac event was counted as an event (according to the defined category, i.e., fatal, non-fatal). Patients presenting with several cardiac events were considered to have only one event for the purposes of calculating overall incidence. In patients with several cardiac events, we considered the most severe cardiac event as the first (i.e., in a case of 2 events, with the first being a grade 1–2 and then a different grade 3–4 event, the latter was considered the event for the purposes of calculations). Time-to-event analyses were calculated in months (from the first treatment after the index date) and were summarized with Kaplan–Meier (KM) curves. For OS, an event was defined as death by any cause, while for EFS, it was failure to achieve CR/CRi (counted on day 1 after starting R/R therapy), relapse after CR/CRi, or death by any cause, whichever occurred first. The impact of risk factors for cardiac events was assessed using multivariate Cox proportional hazard regression. Analyses were conducted in June 2022 using R.2.14 statistical software for all calculations.

3. Results

3.1. Patient Disposition and AML Characteristics

Overall, 571 adult consecutive patients with AML were diagnosed and/or treated at the HULaFe from January 2011 to June 2020. Overall, 46 patients received only supportive care, and 525 patients received various front-line regimens. In total, 285 patients received intensive chemotherapy: 218 (38%) received standard intensive chemotherapy, and 67 (12%) received intensive clinical trials. In total, 240 patients received non-intensive chemotherapy: 7 (1%) received hypomethylating agents, 68 (13%) received LDAC-based regimens, and 165 (31%) underwent non-intensive clinical trials (Table 1).
The median age of patients in the overall cohort (n = 571) was 65 years (range, 18–98 years) (Supplementary Figure S1); 331 (58%) were male; ECOG was >2 in 58 (10%); 350 (61%) were de novo AML and 221 (39%) were secondary or therapy-related AML; 77 (14.7%) had the FLT3-ITD mutation; and 38 (7.2%) received front-line FLT3 inhibitors. Up to 14% of patients had antecedent or ongoing relevant cardiac comorbidities at the time of AML diagnosis, and 38% had cardiac comorbidity (Supplementary Table S1). Patients with relevant cardiac comorbidities were more frequently older than 65 years (70% vs. 49%, p = 0.001) and male (73% vs. 55%, p = 0.004) and more frequently had an ECOG ≥ 2 (34% vs. 23%, p = 0.04) and increased serum creatinine levels (29% vs. 13%, p < 0.001) (Table 2). Intensive chemotherapy was less frequently administered to patients with cardiac comorbidities (23%) as compared with those without cardiac comorbidities (41%) (p = 0.043). Differences between patients receiving intensive chemotherapy versus other approaches are shown in Supplementary Table S2.

3.2. Fatal Cardiac Events in the Overall Cohort

Overall, 26 fatal cardiac events were recorded among 571 patients (crude incidence of 4.6%). The crude incidence of patients experiencing a fatal cardiac event among 46 untreated patients was 15.2% (7 out of 46).
Among 525 treated patients, 19 experienced fatal cardiac events (crude incidence of 3.6%); there was a CI of 2% at 6 months and a CI of 6.7% at 9 years (Figure 1A). Among fatal cardiac events, one was due to an aortic valve rupture (previous endocarditis), one was a cardiac tamponade, eleven were due to heart failure (three of them occurred before starting treatment and three occurred in the follow-up phase), and six were categorized as arrhythmia, although no acute ECG abnormality was found (four cardiorespiratory arrests, one sudden death, and one asystole). In the univariate analysis, patients with prior cardiac antecedents had an increased incidence of fatal cardiac events compared with patients with no prior cardiac disease (CI at 9 years, 20.1% vs. 4.9%; p < 0.001) (Table 3). Multivariate analysis showed that the presence of relevant cardiac antecedents was associated with an increased incidence of fatal cardiac events (hazard ratio (HR), 6.9; confidence interval, 95% (CI 95%), 2.8–16.7; p < 0.001] (Supplementary Table S3).

3.3. Non-Fatal Cardiac Events in the Overall Cohort

The CI of non-fatal cardiac events was 43.7% at 6 months and 56.9% at 9 years (Figure 1B). The 9-year CI of grade 3–4 events was 42.6% and 20.6% for grades 1–2. In the univariate analysis, age ≥ 65 years old (64.4% vs. 49.9%, p < 0.001), relevant cardiac antecedents (73% vs. 54.6%, p = 0.004), and inclusion in a front-line clinical trial (65.2% vs. 50.8%, p < 0.001) were associated with an increased CI of non-fatal cardiac events. Age ≥ 65 (HR 2.2, CI 95% 1.5–3.3, p < 0.001), relevant cardiac antecedents (HR 1.4, CI 95% 1.4–2, p = 0.02), and non-intensive chemotherapy as a front-line treatment (HR 1.8, CI 95% 1.2–2.8, p = 0.004) were associated with an increased CI of non-fatal cardiac events in the multivariate analysis (Supplementary Table S4).
The CI of life-threatening cardiac events was 4.1% at 6 months and 6.4% at 9 years. They occurred more frequently among patients not included in clinical trials and those treated with intensive approaches (not statistically significant) (Table 4).

3.4. Timing of Cardiac Events

Overall, 488 cardiac events occurred in 307 patients during the observation period. Among the 307 patients with at least 1 cardiac event, 38 (12%) developed the first event before starting treatment, 132 (43%) during the first cycle, 31 (10%) during consolidation, 55 (18%) during non-intensive further cycles, 14 (5%) during HSCT, and 37 (12%) during follow-up (Figure 2, Supplementary Table S5; Supplementary Figure S2).

3.5. Cardiac Events in Intensive Versus Non-Intensive Front-Line Cohorts

Among 285 patients treated with intensive chemotherapy, the CI of fatal events was 1.9% at 6 months and 6.3% at 9 years, and the CI of non-fatal events was 42.5% at 6 months and 54.7% at 9 years (Supplementary Table S6). In the univariate analysis, patients with relevant cardiac antecedents and previous treatment with anthracyclines had an increased 9-year CI of fatal cardiac events. Patients aged ≥ 65 years, with relevant cardiac antecedents, and included in the front-line clinical trial had an increased 9-year CI of non-fatal cardiac events.
Among 240 patients treated with non-intensive approaches, the CI of fatal events was 2.2% at 6 months and 6.8% at 9 years; the CI of non-fatal events was 45.2% at 6 months and 59.6% at 9 years (Supplementary Table S7).

3.6. QTc Prolongation Events in the Overall Cohort

The 9-year CI of QTcF prolongations was 11.2% for grades 1–2 and 2.7% for grade 3, and no patient had grades 4 or 5. Among 73 patients who developed QT prolongation, 25 (34%) patients developed them during induction, and 20 (27%) developed them during further non-intensive cycles (Supplementary Figure S3). In the univariate analysis, patients aged ≥65 years, with relevant cardiac antecedents included in the front-line clinical trial or receiving upfront FLT3 inhibitors had an increased 9-year CI of grade 1–2 QTcF prolongations (Supplementary Table S8). No significant associations were found for grade 3 QTcF prolongations.

3.7. Arrhythmia Events in the Overall Cohort

The 9-year CI of grade 1–2 arrhythmia was 2.3%, grade 3–4 was 9.2%, and grade 5 was 1% (six patients died due to suspected or confirmed arrhythmia). Among 64 patients who developed arrhythmias, 29 (45%) did during induction, and 14 (22%) did during non-intensive further cycles (Supplementary Figure S4). In the univariate analysis, patients aged ≥ 65 years with ECOG > 1 had an increased 9-year CI of grade 3–4 arrhythmias (Supplementary Table S9).

3.8. Heart Failure and Myocardial Ischemic Events in the Overall Cohort

The 9-year CI of heart failure events was 1.4% for grades 1–2, 15.3% for grades 3–4, and 2.4% for grade 5 (11 patients died due to heart failure). Among 97 patients who developed cardiac failure events, 52 (54%) patients did during induction, 10 (11%) did during the pre-treatment period, and 13 (15%) did during the post-treatment follow-up period (Supplementary Figure S5). In the univariate analysis, patients with ECOG > 1 had an increased 9-year CI of grade 3–4 heart failure, and patients with relevant cardiac antecedents had an increased 9-year CI of grade 5 heart failure (Supplementary Table S10). Among these 97 patients, 56 (57.7%) had a concomitant infection (i.e., sepsis or pneumonia), and in 24 (24.7%), AML was not in remission.
The 9-year CI of myocardial ischemic events was 2.1% for grades 1–2 and 4.1% for grades 3–4, and no patient had grade 5 (Supplementary Table S11).

3.9. Clinical Outcomes after Intensive Approaches According to the Development of Cardiac Events

The median OS in the overall cohort was 11 months (10–13 months, CI 95%). The CR + CRi rate after intensive induction was not different between those developing or not developing cardiac events (Table 5). Among 285 patients treated with intensive approaches, the median OS was 22 months (16–38 months, CI 95%): 34 months (18 months–NA (not available), CI 95%) in patients without cardiac events (n = 125), 43 months (22 months–NA, CI 95%) with grade 1–2 (n = 52), 15 months (13–22 months, CI 95%) with grade 3–4 (n = 98), and 5 months (3 months–NA, CI 95%) with grade 5 (n = 10) (p < 0.001) (Figure 3A). The median EFS was 10 months (6–13 months, CI 95%): 12 months (10–13 months, CI 95%) in patients without cardiac events, 14 months (6–NA months, CI 95%) in patients with grade 1–2, 8 months (4–13 months, CI 95%) in patients with grade 3–4, and 4 months (3-NA months, CI 95%) in patients with grade 5 (p = 0.015) (Figure 3B).

3.10. Cardiac Events in the 1L Cohort

When censoring patients at first R/R episode (1L cohort), seven patients (1.3%) experienced fatal cardiac events during the observation period, with a CI of 1.2% at 6 months and 2.8% at 9 years (Figure 1C). In the 1L cohort, 222 (42.3%) patients experienced non-fatal cardiac events, with a CI of 37.8% at 6 months and 43.3% at 9 years (Figure 1D). In the univariate analysis, patients with relevant cardiac antecedents (CI at 9 years, 7.4% vs. 1.9%; p < 0.001) and prior exposure to anthracyclines (CI at 9 years, 7.9% vs. 2.4%; p = 0.005) had an increased CI of fatal cardiac events. Patients aged ≥ 65 years (49.4% vs. 37.2%, p = 0.001), with relevant cardiac antecedents (61% vs. 40.5%, p = 0.002), and included in front-line clinical trials (51.8% vs. 36.6%, p < 0.001) had an increased CI of non-fatal cardiac events at 9 years (Supplementary Table S12). Multivariate analysis showed that patients aged ≥ 65 years (HR 1.8, p = 0.007), with relevant cardiac antecedents (HR 1.9, p < 0.001), and undergoing non-intensive chemotherapy (HR 1.9, p = 0.005) were associated with an increased CI of non-fatal cardiac events (Supplementary Table S13).
The CI of grade 1–2 QTcF prolongations was 7.8% at 6 months and 9.2% at 9 years, grade 3 was 1.3% at 6 months and 2% at 9 years, and no patient had grades 4 or 5. In the univariate analysis, patients aged > 65 years (12% vs. 6.4%, p = 0.038), those with relevant cardiac antecedents (16.5% vs. 8.1%, p = 0.018), included in front-line clinical trials (15.3 vs. 4.8, p < 0.001), and treated with FLT3 inhibitors (18.4% vs. 8.4%, p = 0.027) had an increased 9-year CI of grade 1–2 QTcF prolongations.
The 9-year CI of grade 1–2 heart failure events was 1%, for grades 3–4 it was 11.1%, and it was 0.4% for grade 5 (two patients died due to heart failure). In the univariate analysis, patients with relevant cardiac antecedents (19.4% vs. 9.7%, p = 0.017) and with ECOG > 1 (20.6% vs. 8.6%, p < 0.001) had an increased 9-year CI of grade 3–4 heart failure.
The 9-year CI of grade 1–2 arrhythmia was 2%, grade 3–4 was 5.7%, and grade 5 was 0.6% (four patients died due to arrhythmia). In the univariate analysis, patients with relevant cardiac antecedents (12.3% vs. 4.7%, p = 0.009) and an ECOG > 1 (12.2% vs. 4.1%, p = 0.001) had an increased 9-year CI of grade 3–4 arrhythmias.

4. Discussion

Our study shows a high incidence of cardiac events (58.5%) in a real-world series of patients undergoing AML therapy, coupled with significant mortality due to cardiotoxicity. We also show decreased OS and EFS in fit patients developing grade 3–4 cardiotoxic events, while grade 1–2 cardiac events apparently did not impact survival. The presence of relevant cardiac antecedents was the main risk factor for developing fatal cardiac events.
So far, no previous studies have provided a holistic view of cardiac issues in a real-world series of newly diagnosed AML patients followed-up until death or the last patient visit. As a matter of fact, prior studies analyzing cardiac toxicity in AML patients were mainly focused on anthracycline-derived decreases in left ventricular ejection fractions (LVEFs) in adult [7,22] or pediatric [23] patients and/or APL patients [24]. In our study, we analyzed a large series of adult patients with AML, and we focused on all types of cardiac toxicities, not restricted to potential anthracycline-related LVEF decreases. We should highlight that, although this is a real-life study, all patients were treated in a reference institution where a sizable proportion of patients were enrolled in clinical trials in the front line (44%).
Our main objective was to assess the incidence of fatal cardiac events in the overall cohort, and we found an incidence of 4.6% for cardiac-related deaths, rising to 15% among the small percentage (8%) of patients receiving only supportive care. This reflects that some patients cannot start antileukemic therapy due to early death (for instance, due to cardiac events) but also due to comorbidities (such as cardiac comorbidities). When focusing on 525 patients receiving front-line therapy, we observed an estimated CI of 2% at 6 months, which increased to 6.7% at 9 years, reflecting that late fatal cardiac events are frequently occurring among AML patients (due to late toxicities or subsequent treatment lines for R/R episodes). Three out of six patients categorized as having fatal arrhythmias died late, unrelated to front-line therapy (two of them with active diseases), and were recorded as having cardiac arrests.
The estimated cumulative incidence of all types of non-fatal cardiac events was unexpectedly high (57% at 9 years), with a 44% incidence at 6 months, meaning that non-fatal cardiac events occurred early during the treatment phase, with a plateau after 2 years since AML diagnosis. Thus, once patients were successfully treated and off therapy, non-fatal cardiac events were rare, probably due to less exposure to toxic agents, but also reflecting less medical monitoring and reporting. As expected, most of the cardiac events occurred during induction therapy, but post-remission cycles and post-transplant periods were also accompanied by a significant number of events. Most patients in our study developed their first events during the induction or the first cycle, probably due to uncontrolled AML, fluid overload, and severe infections such as pneumonia and sepsis. In fact, we observed that more than half of patients developing a heart failure event had a concomitant relevant infection, in line with the results of a COG AAML0531 pediatric study [23]. Another relevant finding is that, according to CTCAE, most cardiac events were grade ≥ 3, and this was inherent to the CTCAE definitions [18]. To assess the true severity and seriousness of cardiac events, we analyzed the incidence of so-called life-threatening events, resulting in a substantial fatal plus life-threatening crude incidence of 8.7%.
Our study included a detailed analysis of the occurrence of QT prolongation, and as far as we know, this is the largest real-life AML study focusing on this issue. The frequency of all grades of QTcF prolongation in a front-line phase 3 clinical trial with FLT3-inhibitor quizartinib (QuANTUM-First) was 34% in the quizartinib arm and 18% in the control arm. In our analysis, 21% of patients included in front-line clinical trials (the vast majority without FLT3 inhibitors) developed any grade of QTcF prolongation, such as the control arm of the QuANTUM-First trial [25]. In our series, grade 3 QTcF was infrequent (around 2%) in patients either receiving or not receiving FLT3 inhibitors whether included or not in clinical trials, similar to the Quantum-First trial (2% in the quizartinib arm and 1% in the control arm). We can speculate that the higher incidence of QT prolongation among those of our patients who were included in trials was driven not only by the experimental agents’ toxicity but also, at least in part, by the tight ECG monitoring, as per protocol safety procedures. Importantly, in our series, no patient developed a death related to QT prolongation, as per physician judgment.
Heart failure grade 1–2 events were rare, while grade 3–4 events occurred in 12.2% at 6 months and 15.3% at 9 years. It should be noted that most heart failures occurred during induction—coinciding with exposure to anthracyclines and other cardiotoxic agents—with active AML and with the potential co-occurrence of infectious episodes that can trigger some cases into cardiac failure. Interestingly, a COG AAML0531 pediatric study including almost 900 AML patients analyzed the incidence of heart failure (defined as an LVEF decrease) using echocardiographic monitoring. In line with our results, they identified LVEF decreases in 12% of patients during follow-up, 70% of whom experienced onset during the on-protocol period, and a sizable proportion developed heart failure coinciding with sepsis [23]. As serial echocardiogram monitoring was not performed in our study, some low-LVEF events may have been missed, especially in asymptomatic patients. In this regard, a study by Rodríguez-Veiga et al. analyzed late cardiomyopathy via longitudinal assessment using magnetic resonance imaging in 82 patients with acute promyelocytic leukemia treated with anthracycline-containing regimens, showing 12% had developed subclinical cardiomyopathy [26].
In our study, the majority of cardiac events occurred during induction and active therapy phases, suggesting that the late cardiotoxic effects of anthracyclines might be relatively rare. Nevertheless, prior anthracycline exposure is a known factor for developing cardiac toxicity, and we found that this was a risk factor for fatal cardiac events in the univariate analysis when restricted to the first-line cohort. However, we observed increased cardiotoxicity among unfit patients treated without anthracyclines, especially in those with prior cardiac antecedents.
We show that the development of grade 3–4 cardiac events (among patients treated intensively) impacted the OS and EFS, as reported by the COG study [23]. However, we show that the development of isolated grade 1–2 cardiac toxicity had no impact on survival outcomes. Our data highlight that there might be some concern when patients develop cardiac toxicities limited to mild severity, as those seem to be irrelevant to patient outcomes.
There are several studies focused on cardio-protection after treatment with anthracyclines via the use of dexrazoxane, which has been recommended in children since the beginning of anthracycline treatment and in adults after reaching anthracycline doses of 300 mg/m2 [27,28]. Other strategies include the use of liposomal products, angiotensin-converting enzyme (ACE) inhibitors, spironolactone, and β-blockers. A recent systematic review focused mostly on adult patients receiving cardiotoxic drugs (the majority included anthracyclines) found that spironolactone followed by enalapril, nebivolol, and statins was associated with the greatest LVEF improvement, and patients treated with enalapril had a lower risk of developing clinical heart failure [29,30]. These strategies could be applied to patients at a higher risk of developing cardiac events, as found in our study.
We also analyzed cardiac events restricted to the first line (censoring at relapse or refractory status). Interestingly, the rate of all cardiac grade events in 1L patients undergoing intensive chemotherapy was 42.7%, similar to the 46% reported by investigators of a phase 2 206 study assessing the effects of the cardiac repolarization of CPX-351 on patients with acute leukemias [31]. It should be noted that cardiac events were seen in 20% of patients receiving venetoclax with hypomethylating agents for the 1L treatment of AML in 170 less-fit patients treated at the Mayo Clinic [32]. Most events occurred during treatment cycles 1 (41%) and 2 (26%), and 9 patients died due to a cardiac event (5.2%). We can speculate on several reasons explaining the higher incidence of cardiac events in our study: (1) cardiac events were assessed only while treatment was ongoing, so less follow-up was applied; (2) patients were treated outside the context of a clinical trial, and less tight monitoring, including systematic ECG, was probably performed.
Recently, cardiovascular disease has been associated with clonal hematopoiesis, especially TET2 mutations [33,34]. In our institution, routine NGS at diagnosis has been performed since 2017, so we were not able to analyze the association between cardiac events and different mutations in leukemic cells other than FLT3-ITD. On the other hand, it has been hypothesized that genetic variability in genes involved in the anthracycline metabolic pathway could be one of the causes of the differences in clinical outcomes and toxicities such as cardiotoxicity [35]. In a previous study performed on 225 adult de novo AML patients at our institution, we found that a combination of SLC22A16 rs714368 and ABCG2 rs2231142 polymorphisms was related to cardiac toxicity [36].
Although our study is limited by a single-institution retrospective design and baseline echocardiograms or ECGs were not obtained for all patients, we were focused on showing a real-world evidence study. Importantly, our data were not collected in the context of classical clinical trial monitoring, but data were captured and reviewed by experienced hematologists able to medically and homogeneously interpret data from medical records.

5. Conclusions

In summary, our study shows a high incidence of cardiac events through patient journeys in a large, real-world series of 525 patients undergoing AML therapy, with significant mortality due to cardiotoxicity. We have identified prognostic factors that increase the risk of cardiac events, which may be useful in selecting high-risk patients for stringent cardiac monitoring and management. The inclusion of patients in clinical trials could lead to an increased observed incidence of non-fatal cardiac events, probably due to the use of novel cardiotoxic agents, but also due to the inherent tight monitoring of these patients leading to the documentation of cardiac events.

Supplementary Materials

The following supporting information can be downloaded at https://www.mdpi.com/article/10.3390/cancers15082267/s1: Table S1: Baseline cardiac characteristics in all patients; Table S2: Demographic, clinical, and biological characteristics of AML patients at diagnosis according to therapeutic approach (intensive vs. HMA vs. LDAC-based regimens vs. trial vs. BSC); Table S3: Overall cohort: Multivariate analyses of prognostic factors for the development of fatal cardiac events; Table S4: Overall cohort: Multivariate analyses of prognostic factors for the development of non-fatal cardiac events; Table S5: First-line study cohort (from index date to last follow-up): First-line intensive chemotherapy patients only. Crude incidence of first cardiac events (Grade 1–2 vs. grade 3–4 vs. grade 5 vs. no cardiac event) according to time of occurrence (before starting therapy, during induction cycle, consolidation, after stem cell transplant, off therapy/follow-up); Table S6: Intensive chemotherapy patients only: Crude and cumulative incidence of cardiac events (fatal vs. non-fatal vs. no cardiac event) according to demographic, clinical, and biological characteristics of intensive chemotherapy patients at diagnosis; Table S7: Non-intensive chemotherapy patients only: Crude and cumulative incidence of cardiac events (fatal vs. non-fatal vs. no cardiac event) according to demographic, clinical, and biological characteristics of intensive chemotherapy patients at diagnosis; Table S8: Overall study cohort: Crude and cumulative incidence of QTcF prolongation events (grade 0 vs. grade 1–2 vs. grade 3–4 vs. grade 5) according to demographic, clinical, and biological characteristics of patients at diagnosis; Table S9: Overall cohort: Crude and cumulative incidence of arrhythmia (grade 0 vs. grade 1–2 vs. grade 3–4 vs. grade 5) according to demographic, clinical, and biological characteristics of patients at diagnosis; Table S10: Overall study cohort: Crude and cumulative incidence of heart failure and related events (grade 0 vs. grade 1–2 vs. grade 3–4 vs. grade 5) according to demographic, clinical, and biological characteristics of patients at diagnosis; Table S11: Overall study cohort: Crude and cumulative incidence of heart failure and related events (grade 0 vs. grade 1–2 vs. grade 3–4 vs. grade 5) according to demographic, clinical, and biological characteristics of patients at diagnosis; Table S12: First-line cohort (from index date to first refractory/relapse): Crude and cumulative incidence of cardiac events (fatal vs. non-fatal vs. no cardiac event) according to demographic, clinical, and biological characteristics of patients at diagnosis; Table S13: First-line cohort (from index date to first refractory/relapse): Crude and cumulative incidence of cardiac events (fatal vs. non-fatal vs. no cardiac event) according to demographic, clinical, and biological characteristics of patients at diagnosis; Figure S1: Distribution of age at baseline AML diagnosis (diagnosed patients from HULaFe; n = 571); Figure S2: Timing of life-threatening (requiring intensive management) cardiac events in the overall cohort; Figure S3: Timing of QTcF prolongation events in the overall cohort; Figure S4: Timing of arrhythmia events in the overall cohort; Figure S5: Timing of heart failure events in the overall cohort.

Author Contributions

B.B., D.M.-C. (David Martínez-Cuadrón), and P.M. conceived the study. B.B., D.M.-C. (David Martínez-Cuadrón), and P.M. analyzed, interpreted the data, and wrote the paper; B.B., D.M.-C. (David Martínez-Cuadrón), A.S.-A., I.C., E.A.-C., L.T.-M., R.R.-V., I.N.-V., D.M.-C. (David Martínez-Campuzano), R.G.-R., P.L., P.A., A.O.-S., J.A., M.R.-S., F.B.-F., J.E.M.-V., B.M.-H., E.B., C.S., M.S., M.W., C.D., M.A.S., J.D.l.R., and P.M. included the data of patients treated in the institution, reviewed the manuscript, and contributed to the final draft. All authors have read and agreed to the published version of the manuscript.

Funding

Funding for this study was provided by Daiichi Sankyo Inc. in accordance with the Good Publication Practice (GPP3) guidelines. This study was partially supported by the Instituto de Investigación Sanitaria La Fe (2016/0158, 2019/052-1) and the Instituto de Salud Carlos III, 497 Spain: PI18/01340, PI19/00730, FI19/00059.

Institutional Review Board Statement

This study was conducted in accordance with the Declaration of Helsinki, and approved by the Ethics Committee of the Insituto de Investigación Sanitaria La Fe (protocol code, CARDO-LAM, and date of approval, 09/SEP/2020).

Informed Consent Statement

Informed consent was obtained from all subjects involved in the study.

Data Availability Statement

The data presented in this study are available in this article and Supplementary Material.

Conflicts of Interest

P. Montesinos reports these potential conflicts of interest: AbbVie: advisory board, speakers’ bureau, research support; Astellas: research support, consultant, speakers’ bureau, advisory board; Agios: consultant; Tolero Pharmaceutical: consultant; Glycomimetics: consultant; Forma Therapeutics: consultant; Celgene: research support, consultant, speakers’ bureau, advisory board; Daiichi Sankyo: research support, consultant, speakers’ bureau, advisory board; Incyte: speakers’ bureau, advisory board; Janssen: research support, speakers’ bureau, advisory board; Karyopharm: research support, advisory board; Novartis: research support, speakers’ bureau, advisory board; Pfizer: research support, speakers’ bureau, advisory board; Teva: research support, speakers’ bureau, advisory board. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. Dharmani, Wooddell, and Salas are full-time employees of Daiichi Sankyo. All own restricted stock units of Daiichi Sankyo.

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Cancers 15 02267 g001 550
Figure 1. Cumulative incidence of cardiac events. (A) CI of fatal cardiac events in the overall cohort; (B) CI of non-fatal cardiac events in the overall cohort; (C) CI of fatal cardiac events in the 1L cohort; (D) CI of non-fatal cardiac events in the 1L cohort.
Figure 1. Cumulative incidence of cardiac events. (A) CI of fatal cardiac events in the overall cohort; (B) CI of non-fatal cardiac events in the overall cohort; (C) CI of fatal cardiac events in the 1L cohort; (D) CI of non-fatal cardiac events in the 1L cohort.
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Figure 2. Timing of cardiac events in the overall cohort.
Figure 2. Timing of cardiac events in the overall cohort.
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Figure 3. (A) Overall survival in the overall cohort (intensive chemotherapy only) according to the grade of the cardiac event; (B) event-free survival in the overall cohort according to the grade of the cardiac event.
Figure 3. (A) Overall survival in the overall cohort (intensive chemotherapy only) according to the grade of the cardiac event; (B) event-free survival in the overall cohort according to the grade of the cardiac event.
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Table
Table 1. Front-line therapeutic approach in AML patients (intensive vs. non-intensive vs. trial vs. best supportive care).
Table 1. Front-line therapeutic approach in AML patients (intensive vs. non-intensive vs. trial vs. best supportive care).
Therapeutic ApproachScheduleNumber of Patients
n = 571 (%)
Intensive chemotherapy 218 (38)
IDA 1 + Ara-C 2 (3 + 7)195 (34)
IDA + Ara-C (2 + 5)3 (1)
FLAG-IDA (fludarabine + Ara-C + IDA)10 (2)
IDA + Ara-C (3 + 7) + midostaurin4 (1)
Allogeneic transplant4 (1)
Other intensive chemotherapy2 (0.3)
Non-intensive therapy 75 (13)
Azacitidine6 (1)
Decitabine1 (0.2)
FLUGA (fludarabine + LD-Ara-C)68 (12)
Clinical Trial 232 (41)
Intensive 67 (12)
Intensive + FLT3 inhibitor/
placebo		31 (5)
Intensive without FLT3 inhibitor36 (6)
Non-intensive 165 (29)
Non-intensive + FLT3 inhibitor1 (0.2)
Non-intensive without FLT3 inhibitor164 (29)
Supportive care only 46 (8)
1 IDA: idarubicina; 2 Ara-C: cytarabine.
Table
Table 2. Demographic, clinical, and biological characteristics of patients at diagnosis according to previous cardiac comorbidities.
Table 2. Demographic, clinical, and biological characteristics of patients at diagnosis according to previous cardiac comorbidities.
CharacteristicOverallRelevant Cardiac ComorbiditiesNo Cardiac Comorbiditiesp
Median
(Range)		n (%)Median
(Range)		n (%)Median (Range)n (%)
N 571 (100) 82 (14) 489 (86)
Age, years65 (18–98) 72 (37–98) 64 (18–92) <0.001 *
<65 272 (48) 25 (30) 247 (51)0.001
≥65 299 (52) 57 (70) 242 (49)
Gender 571 82 489
Male 331 (58) 60 (73) 271 (55)0.004
Female 240 (42) 22 (27) 218 (45)
ECOG1 (0–4)5711 (0–4)821 (0–4)4890.001 *
0–1 432 (76) 54 (66) 378 (77)0.04
≥2 139 (24) 28 (34) 111 (23)
Comorbidities 571 82 489
Yes 379 (66) 75 (91) 304 (62)<0.001
No 192 (34) 7 (9) 185 (38)
Type of AML 571 82 489
De novo 350 (61) 45 (55) 305 (62)0.298
Therapy-related 91 (16) 13 (16) 78 (16)
Previous MDS/MPN 130 (23) 24 (29) 106 (22)
WHO classification 571 82 489
AML-RGA 154 (27) 20 (24) 134 (27)0.819
AML-NOS 68 (12) 10 (12) 58 (12)
AML-MRC 240 (42) 41 (50) 199 (41)
t-AML 52 (9) 5 (6) 47 (10)
Myeloid sarcoma 2 (0.4) 0 2 (0.4)
Ambiguous lineage 8 (1) 1 (1) 7 (1)
BPDCN 2 (0.4) 0 2 (0.4)
Not available 45 (8) 5 (6) 40 (8)
Extramedullary disease 569 82 487
Yes 100 (18) 14 (17) 86 (18)0.978
No 469 (82) 68 (83) 401 (82)
WBC, ×109/L8 (0.3–434.3)5718.2 (0.3–300.2)828 (0.3–434.3)4890.640 *
≤5 241 (42) 35 (43) 206 (42)0.705
5–10 68 (12) 11 (13) 57 (12)
10–50 153 (27) 24 (29) 129 (26)
> 50 109 (19) 12 (15) 97 (20)
Hemoglobin, g/dL8.8 (2.9–15.5)5718.4 (5–12.9)828.8 (2.9–15.5)4890.078 *
≤10 434 (76) 70 (85) 364 (74)0.044
>10 137 (24) 12 (15) 125 (26)
Platelet count, ×109/L53 (1–1442)57047 (8–816)8154 (1–1442)4890.275 *
≤20 109 (19) 16 (20) 93 (19)0.997
> 20 461 (81) 65 (80) 396 (81)
PB blasts, % 563 82 481
≤50 417 (74) 64 (78) 353 (73)0.451
> 50 146 (26) 18 (22) 128 (27)
BM blasts, %47 (0–100)55943 (14–100)8148 (0–100)4780.239 *
≤30 158 (28) 28 (35) 130 (27)0.339
>30≤70 234 (42) 29 (36) 205 (43)
>70 167 (30) 24 (30) 143 (30)
Creatinine, mg/dL0.9 (0.1–7.4)5701 (0.2–5.4)820.8 (0.1–7.4)488<0.001 *
≤1.3 482 (85) 58 (71) 424 (87)<0.001
> 1.3 88 (15) 24 (29) 64 (13)
Urea, mg/dL36 (6–174)55445 (18–158)8135 (6–174)473<0.001 *
≤50 429 (77) 46 (57) 383 (81)<0.001
>50 125 (23) 35 (43) 90 (19)
Uric acid, mg/dL5 (1–29)4895.8 (1.4–17.1)684.9 (1–29)4210.002 *
≤7 398 (81) 48 (71) 350 (83)0.022
>7 91 (19) 20 (29) 71 (17)
Bilirubin, mg/dL0.6 (0.1–6.2)5530.7 (0.1–2.3)770.6 (0.1–6.2)4720.313 *
≤1.2 484 (88) 68 (86) 416 (88)0.813
>1.2 69 (12) 11 (14) 58 (12)
AST, U/L22 (6–1085)54922 (7–185)7722 (6–1085)4720.061 *
≤50 482 (88) 69 (90) 413 (88)0.821
>50 66 (12) 8 (10) 58 (12)
ALT, U/L18 (3–714)56616 (3–125)8019 (3–714)4860.68 *
≤50 504 (89) 75 (94) 429 (88)0.207
>50 62 (11) 5 (6) 57 (12)
Albumin, g/dL3.7 (1.6–5.2)5183.7 (2.2–4.8)693.7 (1.6–5.2)4490.148 *
≤3.5 204 (39) 31 (45) 173 (39)0.379
>3.5 314 (61) 38 (55) 276 (61)
LDH, U/L521 (101–42630)558627 (149–11800) 509 (101–42630) 0.984 *
≤600 307 (55) 38 (48) 269 (56)0.181
>600 251 (45) 42 (53) 209 (44)
Fibrinogen487 (34–1150)548493 (162–1002)79487 (34–1150)4690.947 *
≤170 6 (1) 1 (1) 5 (1)0.67
>170 542 (99) 78 (99) 464 (99)
Prothrombin time 501 70 431
Prolonged 175 (35) 33 (47) 142 (33)0.03
Normal 326 (65) 37 (53) 289 (67)
APTT 541 76 465
Prolonged 73 (13) 10 (13) 63 (14)0.917
Normal 468 (87) 66 (87) 401 (86)
Cytogenetics 571 82 489
Normal 211 (37) 34 (41) 177 (36)0.8
Abnormal 293 (51) 38 (46) 255 (52)
No metaphases 47 (8) 7 (9) 40 (8)
Not available 20 (4) 3 (4) 17 (3)
MRC Cytogenetic risk 517 74 443
Favorable 27 (5) 1 (1) 26 (6)0.185
Intermediate 307 (59) 49 (66) 258 (58)
Adverse 183 (35) 24 (32) 159 (36)
FLT3-ITD 517 77 440
Positive 79 (15) 9 (12) 70 (16)0.437
Negative 438 (85) 68 (88) 370 (84)
FLT3-ITD ratio 517 77 440
<0.05 452 (87) 70 (91) 382 (87)0.752
0.05–0.5 24 (5) 2 (3) 22 (5)
0.5–0.8 23 (4) 3 (4) 20 (5)
≥0.8 18 (3) 2 (3) 16 (4)
FLT3-TKD 505 75 430
Positive 26 (13) 6 (8) 20 (5)0.217
Negative 479 (93) 69 (92) 410 (93)
NPM1 514 77 437
Positive 119 (23) 18 (23) 101 (23)0.924
Negative 395 (77) 59 (77) 336 (77)
CEBPA 571 82 489
Positive 14 (2) 2 (2) 12 (2)0.427
Negative 317 (56) 47 (57) 270 (55)
Not available 240 (42) 33 (40) 207 (42)
IDH 571 82 489
IDH1 positive 23 (4) 3 (4) 20 (4)0.67
IDH2 positive 54 (9) 6 (7) 48 (10)
Negative 266 (47) 43 (52) 223 (46)
Not available 228 (40) 30 (37) 198 (40)
Therapeutic approach 571 82 489
Intensive 218 (38) 19 (23) 199 (41)0.043
HMA 7 (1) 2 (2) 5 (1)
LDAC-based 68 (12) 11 (13) 57 (12)
Clinical trial, intensive 67 (12) 9 (11) 58 (12)
Clinical trial, non-intensive 165 (29) 32 (39) 133 (27)
BSC 46 (8) 9 (11) 37 (8)
* p compares continuous variables.
Table
Table 3. Overall cohort: Crude and cumulative incidence of cardiac events (fatal vs. non-fatal vs. no cardiac event) according to demographic, clinical, and biological characteristics of patients at diagnosis.
Table 3. Overall cohort: Crude and cumulative incidence of cardiac events (fatal vs. non-fatal vs. no cardiac event) according to demographic, clinical, and biological characteristics of patients at diagnosis.
No Cardiac eventp-Value *Fatal Cardiac EventNon-Fatal Cardiac Event
CharacteristicN (%) Crude incidence
N (%)		Cumulative IncidenceCrude incidence
N (%)		Cumulative Incidence
At 6 months, %At Last FU, %pAt 6 months, %At Last FU, %p
N218 (42) 19 * (3.6)26.7 288 (54.9)43.756.9
Age, N218 19 288
<65 years128 (48.5)0.0059 (3.4)1.65.90.25127 (48.1)37.849.9<0.001
≥65 years90 (34.5) 10 (3.8)2.56.8 161 (61.7)49.764.4
Relevant cardiologic antecedents218 19 288
No202 (44.7)<0.00110 (2.2)1.24.9<0.001240 (53.1)41.854.60.004
Yes16 (21.9) 9 (12.3)7.220.1 48 (65.8)56.473
All cardiologic antecedents218 19 288
No163 (49.7)<0.0016 (1.8)0.74.10.0004159 (48.5)37.649.7<0.001
Yes55 (27.9) 13 (6.6)4.410.7 129 (65.5)54.270.6
Previous anthracycline treatment218 19 288
No207 (42.1)0.5117 (3.5)1.76.50.22268 (54.5)43.356.30.49
Yes11 (33.3) 2 (6.1)6.76.7 20 (60.6)50.560.7
ECOG at diagnosis218 19 288
<2173 (41.4)0.9915 (3.6)26.70.56230 (55)43.357.20.63
≥245 (42.1) 4 (3.7)1.95.5 58 (54.2)45.556.1
FLT3-ITD status197 19 269
Negative161 (39.5)0.4417 (4.2)2.37.80.53230 (56.4)44.258.80.52
Positive36 (46.8) 2 (2.6)1.43.1 39 (50.7) 43.151.3
Treatment approach218 19 288
Intensive125 (43.9)0.510 (3.5)1.96.30.37150 (52.6)42.554.70.17
Non-intensive93 (38.8) 9 (3.8)2.26.8 138 (57.5)45.259.6
Inclusion in clinical trial218 19 288
No140 (47.8)0.0048 (2.7)1.15.40.06145 (49.5)37.450.8<0.001
Yes78 (33.6) 11 (4.7)3.26.5 143 (61.6)51.865.2
Use of FLT3 inhibitors218 19 288
No204 (41.9)0.7518 (3.7)2.24.80.76265 (54.4)42.856.30.28
Yes14 (36.8) 1 (2.6)03.1 23 (60.5)55.360.7
* This p-value compares the crude incidence between the three groups. FU: follow-up
Table
Table 4. Overall cohort: Crude and cumulative incidence of cardiac events (fatal vs. life-threatening vs. no cardiac event) according to demographic, clinical, and biological characteristics of patients at diagnosis.
Table 4. Overall cohort: Crude and cumulative incidence of cardiac events (fatal vs. life-threatening vs. no cardiac event) according to demographic, clinical, and biological characteristics of patients at diagnosis.
No Cardiac Eventp-Value *Non-Life-ThreateningLife-ThreateningFatal
CharacteristicN (%) Crude Incidence
N (%)		Cumulative IncidenceCrude Incidence
N (%)		Cumulative IncidenceCrude Incidence
N (%)		Cumulative Incidence
At 6 months, %At Last FU, %pAt 6 months, %At Last FU, %pAt 6 months, %At Last FU, %p
N218 (41.5) 261 (49.7)4053.1 27 (5.1)4.16.4 19 (3.6)26.7
Age218 261 27 19
<65 years128 (48.5)<0.001108 (40.9)32.943.8<0.00119 (7.2)4.78.20.119 (3.4)1.65.90.25
≥65 years90 (34.5) 153 (58.6)47.262.9 8 (3.1)3.53.5 10 (3.8)2.56.8
Relevant cardiologic antecedents218 261 27 19
No202 (44.7)<0.001215 (47.6)37.950.40.00125 (5.5)4.46.60.4510 (2.2)1.24.9<0.001
Yes16 (21.9) 46 (63)53.471.7 2 (2.7)1.94 9 (12.3)7.220.1
Previous anthracycline treatment218 261 27 19
No207 (42.1)0.6242 (49.2)39.552.30.3326 (5.3)4.16.50.717 (3.5)1.76.50.22
Yes11 (33.3) 19 (57.6)47.558.8 1 (3)44 2 (6.1)6.76.7
ECOG at diagnosis218 261 27 19
<2173 (41.4)0.99209 (50)39.653.30.621 (5)3.86.10.5615 (3.6)26.70.56
≥245 (42.1) 52 (48.6)41.352.6 6 (5.6)5.57.5 4 (3.7)1.95.5
FLT3-ITD status197 243 26 19
Negative161 (39.5)0.55209 (51.2)40.355.30.3421 (5.2)4.26.10.6317 (4.2)2.37.80.53
Positive36 (46.8) 34 (44.2)38.245.5 5 (6.5)5.67.3 2 (2.6)1.43.1
Treatment chemotherapy218 261 27 19
Intensive125 (43.9)0.07130 (45.6)37.648.80.02520 (7)4.88.30.08810 (3.5)1.96.30.37
Non-intensive93 (38.8) 131 (54.6)42.758.1 7 (2.9)3.23.2 9 (3.8)2.26.8
Inclusion in clinical trial218 261 27 19
No140 (47.8)<0.001125 (42.7)32.845.4<0.00120 (6.8)580.0968 (2.7)1.15.40.06
Yes78 (33.6) 136 (58.6)49.263.5 7 (3)2.93.5 11 (4.7)3.26.5
Use of FLT3 inhibitors2180.71261 27 19
No204 (41.9) 239 (49.1)38.952.30.1926 (5.3)4.26.70.4318 (3.7)2.24.80.76
Yes14 (36.8) 22 (57.9)5358.8 1 (2.6)2.72.7 1 (2.6)03.1
* This p-value compares the crude incidence between the four groups. FU: follow-up.
Table
Table 5. Main clinical outcomes (CR/CRi/OS/EFS) in intensive chemotherapy AML patients according to the occurrence of their first cardiac events (grade 0 vs. 1–2 vs. 3–4 vs. 5) from index date to last follow-up.
Table 5. Main clinical outcomes (CR/CRi/OS/EFS) in intensive chemotherapy AML patients according to the occurrence of their first cardiac events (grade 0 vs. 1–2 vs. 3–4 vs. 5) from index date to last follow-up.
All PatientsCardiac EventNo Cardiac Eventp
Grades 1–2Grades 3–4Grade 5
N (%)N (%)N (%)N (%)N (%)
Response, n (%)285 (100)52 (100)98 (100)10 (100)125 (100)
ORR (CR + CRi)193 (67.7) 39 (75)62 (63.3)8 (80)84 (67.2)0.426
CR184 (64.6)35 (67.3)58 (59.2)7 (70)84 (67.2)
CRi9 (3.1)4 (7.7)4 (4.1)1 (10)0 (0)
PR12 (4.2)1 (1.9)4 (4.1)0 (0)7 (5.6)
Resistance50 (17.5)10 (19.2)19 (19.4)1 (10)20 (16)
Induction death30 (10.5)2 (3.8)13 (13.3)1 (10)14 (11.2)
OS, n (%)
Median (CI95), months21.6 (15.5–37.5)43.2 (21.6-NA)14.8 (13–21.5)5.2 (2.8-NA)34.2 (18-NA)<0.001
1 year (CI95), %63 (57–68)73 (62–86)59 (50–70)30 (12–77)64 (56–73)
2 years (CI95), %48 (42–54)59 (47–74)38 (29–49)20 (6–69)64 (46–64)
3 years (CI95), %44 (39–50)57 (44–72)34 (25–45)20 (6–69)50 (42–60)
5 years (CI95), %39 (34–46)48 (35–65)30 (22–42)10 (2–64)47 (39–57)
EFS, n (%)
Median (CI95), months10.2 (6.4–13)13.9 (5.5-NA)7.5 (4.1–12.6)3.5 (2.8-NA)12.3 (5.7–48)0.015
1 year (CI95), %46 (41–52)50 (38–66)42 (33–53)20 (6–69)50 (42–60)
2 years (CI95), %36 (31–42)48 (36–64)24 (17–35)20 (6–69)42 (35–52)
3 years (CI95), %34 (29–40)43 (32–60)23 (16–33)10 (2–64)41 (34–51)
5 years (CI95), %32 (26–38)40 (29–57)23 (16–33)10 (2–64)38 (30–48)
ORR: overall response rate; OS: overall survival; EFS: event-free survival.
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MDPI and ACS Style

Boluda, B.; Solana-Altabella, A.; Cano, I.; Martínez-Cuadrón, D.; Acuña-Cruz, E.; Torres-Miñana, L.; Rodríguez-Veiga, R.; Navarro-Vicente, I.; Martínez-Campuzano, D.; García-Ruiz, R.; et al. Incidence and Risk Factors for Development of Cardiac Toxicity in Adult Patients with Newly Diagnosed Acute Myeloid Leukemia. Cancers 2023, 15, 2267. https://doi.org/10.3390/cancers15082267

AMA Style

Boluda B, Solana-Altabella A, Cano I, Martínez-Cuadrón D, Acuña-Cruz E, Torres-Miñana L, Rodríguez-Veiga R, Navarro-Vicente I, Martínez-Campuzano D, García-Ruiz R, et al. Incidence and Risk Factors for Development of Cardiac Toxicity in Adult Patients with Newly Diagnosed Acute Myeloid Leukemia. Cancers. 2023; 15(8):2267. https://doi.org/10.3390/cancers15082267

Chicago/Turabian Style

Boluda, Blanca, Antonio Solana-Altabella, Isabel Cano, David Martínez-Cuadrón, Evelyn Acuña-Cruz, Laura Torres-Miñana, Rebeca Rodríguez-Veiga, Irene Navarro-Vicente, David Martínez-Campuzano, Raquel García-Ruiz, and et al. 2023. "Incidence and Risk Factors for Development of Cardiac Toxicity in Adult Patients with Newly Diagnosed Acute Myeloid Leukemia" Cancers 15, no. 8: 2267. https://doi.org/10.3390/cancers15082267

APA Style

Boluda, B., Solana-Altabella, A., Cano, I., Martínez-Cuadrón, D., Acuña-Cruz, E., Torres-Miñana, L., Rodríguez-Veiga, R., Navarro-Vicente, I., Martínez-Campuzano, D., García-Ruiz, R., Lloret, P., Asensi, P., Osa-Sáez, A., Aguero, J., Rodríguez-Serrano, M., Buendía-Fuentes, F., Megías-Vericat, J. E., Martín-Herreros, B., Barragán, E., ... Montesinos, P. (2023). Incidence and Risk Factors for Development of Cardiac Toxicity in Adult Patients with Newly Diagnosed Acute Myeloid Leukemia. Cancers, 15(8), 2267. https://doi.org/10.3390/cancers15082267

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