Distribution of Xanthomonas oryzae pv. oryzae Pathotypes in Basmati-Rice-Growing Areas of Jammu and Kashmir, India

Round 1

Reviewer 1 Report

Manuscript entitled “Distribution of Xanthomonas oryzae pv. oryzae Pathotypes in Basmati Growing Areas of Jammu and Kashmir, India” submitted buy Tabasia et al, is of significance in the specific field of research. Work has been performed well and presented in accurate way.  

Introduction section has been written in perfect way all the information required to supplement the study has been mentioned with proper references, authors should check this section for grammar improvements.

Methodology

All the information required is available in this section; also scientific style of methodology section is fine. I have comments regarding typing and grammatical check of this section.

Results and discussion

Presentation of finding in the result section and their interpretation in the discussion has been done very well with proper supporting references.

References are fine these include new to old references,

I have suggestion if authors add few more new references in the supporting evidenced of results.

 

Overall manuscript can be accepted after minor grammatical and typing improvements

Author Response

Dear Sir,

Thanks for highlighting the corrections. All the suggestions are incorporated in the revised manuscript. 

Author Response File: Author Response.pdf

Reviewer 2 Report

This is a nice study that need a few minor edits.

Line 26: add 's' to pathogen

Line 26: change 'was' to were

Line 28: in 'a' before short

Line 30: add 'a' after showed

Line 33: change proved to confirmed

Line 33: insert 'the' before leaf

Line 34: remove 's' from postulate

Line 34: replace were with 'was'

Line 44: insert 'the' before highest

Line 55: replace 'in' with the

Line 55: remove 'it'

Line 98: replace 'are' with is

Line 98: remove 's' from evidence

Line 109: replace 'have' with has

Line 156: superscript 'o' after 4

Line 187: superscript 'o' after 37

Line 192: insert a space between 100 and ng

Line 194: insert a space between 20 and ul

2.3.3 Pathogenicity assay section: another way to confirm Xoo is via the use of the - Lang JM, Hamilton JP, Diaz MGQ, Van Sluys MA, Burgos MRG, Vera Cruz CM, Buell CR, Tisserat NA, Leach JE. Genomics-Based Diagnostic Marker Development for Xanthomonas oryzae pv. oryzae and X. oryzae pv. oryzicola. Plant Dis. 2010 Mar;94(3):311-319. doi: 10.1094/PDIS-94-3-0311. PMID: 30754246.

Line 208: replace 'proving' with confirming

Line 212: insert a space between 14 and cm

Line 215: superscript 7 an 8

Were the bacteria enumerated for this part of the work

Line 217: were the scissors sterile? if they are state that

Line 227: insert 'a' before pure

Line 228: replace 'prove' with confirm

Line 243: add 's' to culture

Line 244: superscript 7 and 8

Line 281: remove 'medium'

Line 283-284: this sentence needs rewriting it is not clear what you are trying to do

Line 294: remove 'medium'

Line 295: remove 'agar'

Line 300: remove 's' from gram

Line 300: add 'the' after retained

Line 308: insert 'the' before leaf

Line 308: add 'and' after leaf and remove 'of rice'

For the identification of Xoo using 16S you need to add an outgroup such as Xanthomonas oryzae pv oryzicola. Include an outgroup and reanalyse and generate a new figure please.

Line 322: remove 'of the' and replace with 'with'

Line 323-333: insert a space between the number and 'cm'

Table 4: need to say that the measurement is lesion length and that its cm

Line 349: insert 'the' after of

Section 3.4: can these results be included as a table as this really hard to read

Table 5: This table needs to be self explanatory, it is really hard to understand

Section 3.5: can this data be presented as table

Line 473-474: Can you please rewrite this sentence it is not clear what you are trying to say

Line 522-528: 16S sequencing cannot be used to infer evolutionary history, it can be used for identification of isolates, but its not even great at doing that. Either remove the evolutionary history context or do analysis which will allow for evolutionary evaluation

Line 542: insert 'a' before new

Line 564: insert 'the' before majority

 

  

 

Author Response

Dear Sir,

All the suggestions are incorporated in the revised MS.

S.no.	Referee comments	Response
1	For the identification of Xoo using 16S you need to add an outgroup such as Xanthomonas oryzae pv oryzicola. Include an outgroup and reanalyse and generate a new figure please.	Modify & Incorporated
2	Table 4: need to say that the measurement is lesion length and that its cm	Done
3	Section 3.4: can these results be included as a table as this really hard to read	Modify
4	Table 5: This table needs to be self-explanatory, it is really hard to understand
5	Section 3.5: can this data be presented as table	Table added
6	16S sequencing cannot be used to infer evolutionary history, it can be used for identification of isolates, but its not even great at doing that. Either remove the evolutionary history context or do analysis which will allow for evolutionary evaluation	Removed

regards

Author Response File: Author Response.docx

Reviewer 3 Report

This manuscript deals with distribution of Xanthomonas oryzae pv. oryzae pathotypes in basmati growing areas of Jammu and Kashmir, India. Some basic weaknesses are detected in the manuscript as below: 

Comment #1: We advise that you should add pictures of BLB of rice, Xoo isolated strains colony and inoculated diseased leaves in the manuscript.

Comment #2: The Latin scientific names of strains on phylogenetic trees need to be italicized.

Comment #3: We advise that you should add another gene sequence to conduct molecular identification of Xoo and construct phylogenetic tree.

Comment #4: Present the final conclusion and practical suggestions in the end of abstract part. 

Comment #5: Research innovation is not explicitly stated.

Comment #6: In the end of abstract and conclusion parts you must highlight the practical suggestions of your study. 

Author Response

Dear Sir,

Add the BLB pics, modify the phylogenetic tree, and modify the abstract and conclusion.

 

regards