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Agronomy
Volume 15
Issue 2
10.3390/agronomy15020294
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Sly-miR398 Participates in Heat Stress Tolerance in Tomato by Modulating ROS Accumulation and HSP Response

by
Baoyu Li
1,
Peiwen Wang
1,
Shuaijing Zhao
1,
Jiaqi Dong
1,
Shengming Mao
1,
Xuyongjie Zhu
1,
Tiantian Yuan
1,
Haiying Qiu
1,
Long Cao
1,
Yunmin Xu
1,2,3,
Yong He
1,2,3,
Zhujun Zhu
1,2,3 and
Guochao Yan
1,2,3,*
1
College of Horticulture Science, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China
2
Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable, Ministry of Agriculture and Rural Affairs, Hangzhou 311300, China
3
Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, Hangzhou 311300, China
*
Author to whom correspondence should be addressed.
Agronomy 2025, 15(2), 294; https://doi.org/10.3390/agronomy15020294
Submission received: 19 December 2024 / Revised: 21 January 2025 / Accepted: 21 January 2025 / Published: 24 January 2025
(This article belongs to the Section Horticultural and Floricultural Crops)
Versions Notes

Abstract

Heat stress is one of the most important environmental problems in agriculture, which severely restricts the growth and yield of plants. In plants, microRNA398 (miR398) negatively regulates the activity of superoxide dismutase (SOD) by modulating the expression of its coding genes (CSDs) post-transcriptionally, thereby regulating reactive oxygen species (ROS) homeostasis and stress resistance. In this study, the role of miR398 in heat stress tolerance in tomatoes was investigated. Under heat stress, the expression of miR398 was upregulated in tomatoes, while the expression of its target genes (CSD1 and CSD2) and SOD activity was downregulated. Furthermore, by comparing the heat stress response in wild type (WT) and a transgenic line overexpressing MIR398 (miR398-OE), the results showed that overexpression of miR398 promoted tomato growth and the expression of genes encoding heat shock factor (HSF, transcription factor) and heat shock protein (HSP) under heat stress. Meanwhile, downregulated activity of antioxidant enzymes, including SOD, catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX), and enhanced ROS accumulation was observed in miR398-OE compared with that in WT under heat stress. Further study using dimethylthiourea (DMTU, a ROS scavenger) indicated that the enhanced plant growth and expression of HSFs/HSPs was based on the promoted accumulation of ROS in miR398-OE. Overall, the results of this study revealed that the upregulated expression of miR398 in response to heat stress would modulate the antioxidant system and enhance ROS accumulation, thereby enhancing the expression of HSFs and HSPs and heat stress tolerance in tomatoes.
Keywords: microRNA; heat stress acclimation; antioxidant system; ROS balance; feedback loop microRNA; heat stress acclimation; antioxidant system; ROS balance; feedback loop

Share and Cite

MDPI and ACS Style

Li, B.; Wang, P.; Zhao, S.; Dong, J.; Mao, S.; Zhu, X.; Yuan, T.; Qiu, H.; Cao, L.; Xu, Y.; et al. Sly-miR398 Participates in Heat Stress Tolerance in Tomato by Modulating ROS Accumulation and HSP Response. Agronomy 2025, 15, 294. https://doi.org/10.3390/agronomy15020294

AMA Style

Li B, Wang P, Zhao S, Dong J, Mao S, Zhu X, Yuan T, Qiu H, Cao L, Xu Y, et al. Sly-miR398 Participates in Heat Stress Tolerance in Tomato by Modulating ROS Accumulation and HSP Response. Agronomy. 2025; 15(2):294. https://doi.org/10.3390/agronomy15020294

Chicago/Turabian Style

Li, Baoyu, Peiwen Wang, Shuaijing Zhao, Jiaqi Dong, Shengming Mao, Xuyongjie Zhu, Tiantian Yuan, Haiying Qiu, Long Cao, Yunmin Xu, and et al. 2025. "Sly-miR398 Participates in Heat Stress Tolerance in Tomato by Modulating ROS Accumulation and HSP Response" Agronomy 15, no. 2: 294. https://doi.org/10.3390/agronomy15020294

APA Style

Li, B., Wang, P., Zhao, S., Dong, J., Mao, S., Zhu, X., Yuan, T., Qiu, H., Cao, L., Xu, Y., He, Y., Zhu, Z., & Yan, G. (2025). Sly-miR398 Participates in Heat Stress Tolerance in Tomato by Modulating ROS Accumulation and HSP Response. Agronomy, 15(2), 294. https://doi.org/10.3390/agronomy15020294

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