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Microorganisms
Volume 13
Issue 2
10.3390/microorganisms13020300
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Article

Taxane-Producing Fungi Isolated from Taxus globosa Tree Bark

by
Jocelyn Guadalupe Guevara-Sánchez
1,
María Guadalupe Aguilar-Uscanga
1,
Carlos Augusto Ledesma-Escobar
2,
Claudia Castro-Martínez
3,
Renaud Condé
4,
Bernardo Sachman-Ruíz
5,* and
Sandra del Moral
1,*
1
Laboratorio de Bioingeniería-UNIDA, Tecnológico Nacional de México, Instituto Tecnológico de Veracruz, Veracruz 91897, Veracruz, Mexico
2
Departamento de Química Analítica, Campus Rabanales, Universidad de Córdoba, 14071 Cordoba, Spain
3
Departamento de Biotecnología Agrícola, Instituto Politécnico Nacional, Centro Interdisciplinario de Investigación Para el Desarrollo Integral Regional, Unidad Sinaloa, Guasave 81100, Sinaloa, Mexico
4
Centro de Investigaciones Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca 62100, Morelos, Mexico
5
Laboratorio de Artropodología del INIFAP CENID Salud Animal e Inocuidad, Jiutepec 62550, Morelos, Mexico
*
Authors to whom correspondence should be addressed.
Microorganisms 2025, 13(2), 300; https://doi.org/10.3390/microorganisms13020300
Submission received: 30 November 2024 / Revised: 14 December 2024 / Accepted: 14 December 2024 / Published: 29 January 2025
(This article belongs to the Special Issue Fungal Metabolites: Powering Pharmacological and Agricultural Frontiers)
Versions Notes

Abstract

The taxane molecules extracted from the bark of trees from the Taxus genus demonstrate anticancer activity and are the main components of the drug paclitaxel. Even though a great deal of research has been carried out to produce them synthetically, this manufacturing is still dependent on Taxus cell culture. Furthermore, these processes are not suitable for steady taxane production. Therefore, the search for alternative sources of taxane production has generated growing interest amongst the scientific community. The use of endophytic fungi for the obtention of taxane constitutes an attractive alternative. Here, we present an analysis of the taxane production of several microorganisms through LC–QTOF MS/MS. We isolated 21 endophytic fungus strains, identified through sequencing of their internal transcribed spacer region (ITS). The phylogenetic analysis showed the presence of 11 different Fungus genera, namely Aspergillus, Trichoderma, Neurospora, Penicillium, Curvularia, Arcopilus, Biscogniauxia, Hypoxylon, Sordaria, Xylariales, and Gelasinospora. These fungi have been previously described to synthetize distinct metabolites of medical interest, hence supporting the study of their potential taxane production. Here, we report the production of taxadiene by some of these fungi, namely Aspergillus sp. (1.35 μg/L), TgO (0.7 μg/L), Trichoderma harzianum (0.13 μg/L), and Hypoxylon sp. (0.3 μg/L). Furthermore, we also detected the production of baccatin III, a crucial precursor component of the drug paclitaxel. This is the first report of taxane production by fungi phylogenetically related to the Arcopilus and Endoxyla genera.
Keywords: taxane; endophytic fungi; Taxus globose; baccatin III; paclitaxel taxane; endophytic fungi; Taxus globose; baccatin III; paclitaxel

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MDPI and ACS Style

Guevara-Sánchez, J.G.; Aguilar-Uscanga, M.G.; Ledesma-Escobar, C.A.; Castro-Martínez, C.; Condé, R.; Sachman-Ruíz, B.; del Moral, S. Taxane-Producing Fungi Isolated from Taxus globosa Tree Bark. Microorganisms 2025, 13, 300. https://doi.org/10.3390/microorganisms13020300

AMA Style

Guevara-Sánchez JG, Aguilar-Uscanga MG, Ledesma-Escobar CA, Castro-Martínez C, Condé R, Sachman-Ruíz B, del Moral S. Taxane-Producing Fungi Isolated from Taxus globosa Tree Bark. Microorganisms. 2025; 13(2):300. https://doi.org/10.3390/microorganisms13020300

Chicago/Turabian Style

Guevara-Sánchez, Jocelyn Guadalupe, María Guadalupe Aguilar-Uscanga, Carlos Augusto Ledesma-Escobar, Claudia Castro-Martínez, Renaud Condé, Bernardo Sachman-Ruíz, and Sandra del Moral. 2025. "Taxane-Producing Fungi Isolated from Taxus globosa Tree Bark" Microorganisms 13, no. 2: 300. https://doi.org/10.3390/microorganisms13020300

APA Style

Guevara-Sánchez, J. G., Aguilar-Uscanga, M. G., Ledesma-Escobar, C. A., Castro-Martínez, C., Condé, R., Sachman-Ruíz, B., & del Moral, S. (2025). Taxane-Producing Fungi Isolated from Taxus globosa Tree Bark. Microorganisms, 13(2), 300. https://doi.org/10.3390/microorganisms13020300

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