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Article

Zinc Finger Protein 1 (ZFP1) Is Involved in Trichome Initiation in Arabidopsis thaliana

by
Aidong Zhang
1,2,
Yihua Liu
1,
Chunyan Yu
1,
Linli Huang
1,
Minjie Wu
1,
Junyu Wu
1 and
Yinbo Gan
1,*
1
Zhejiang Key Lab of Crop Germplasm, Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
2
Shanghai Key Laboratory of Protected Horticultural Technology, Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
*
Author to whom correspondence should be addressed.
Agriculture 2020, 10(12), 645; https://doi.org/10.3390/agriculture10120645
Submission received: 17 October 2020 / Revised: 11 December 2020 / Accepted: 16 December 2020 / Published: 18 December 2020
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Abstract

:
Arabidopsis trichome is specialized structure that develops from epidermal cells, and is an excellent model system for studying various aspects of plant cell development and cell differentiation. Our previous studies have shown that C2H2 zinc finger protein family genes, including GIS, GIS2, GIS3, ZFP5, ZFP6 and ZFP8, play an important role in controlling trichome initiation in Arabidopsis. Here, our novel results showed a C2H2 zinc finger protein, ZFP1, which also plays an important role in trichome initiation in Arabidopsis. ZFP1 over-expression lines display significantly increased trichome number on cauline leaves, lateral branches and main stems in comparison with wild type plants. ZFP1 RNAi lines and loss-of-function mutants showed the opposite phenotype. Furthermore, our study also found that ZFP1 mediates the regulation of trichome initiation by cytokinin signaling. The molecular and genetic analyses reveal that ZFP1 acts upstream of key trichome initiation factors, GL3 and TRY.

1. Introduction

Trichome, covering on the surface of plants’ most aerial parts, could protect plants from many kinds of adverse environments [1,2,3,4,5]. The distribution of trichomes on abaxial and adaxial surface of rosette leaves can also be a phase marker for the vegetative growth phase. The first few rosette leaves only produce trichomes on the adaxial side. The later leaves produce trichomes on both the adaxial side and abaxial side. Based on this feature, a vegetative growth phase can be subdivided into a juvenile phase during which trichomes are only produced on the adaxial side, and an adult phase with trichomes on both sides [6].
Classic molecular and genetic analyses have identified the main regulators in Arabidopsis. They are divided into positive regulators whose mutants cover less trichomes, and negative regulators whose mutants display more or clustered trichomes [7]. In Arabidopsis, positive regulators mainly belong to three transcription factor groups, including the WD40 repeat protein [8], the R2R3 MYBs [9,10,11] and the basic helix-loop-helix (bHLH) factors [12,13]. They form a trimetric activator complex, which is called the MYB-bHLH-WD40 complex, and then combine with the promoter of GLABRA2 (GL2) to regulate the trichome initiation [14,15]. A group of single-repeat R3 MYBs, such as CAPRICE (CPC), TRIPTYCHON (TRY), ENHANCER OF TRY AND CPC 1(ETC1), ETC2, ETC3, TRICHOMELESS1(TCL1) and TCL2, act as the negative regulators [16,17,18,19,20,21,22,23]. They compete with R2R3 MYBs to bind to bHLH factors and form a repressor complex [15,24,25,26,27,28]. It is known that trichome development is a very complicated network regulation system. At present, two theoretical models were proposed: the activator–inhibitor model and activator–depletion model. According to activator–inhibitor model, the activators can activate their own inhibitors and up-regulate the expression of them [29,30]. This characteristic leads to the increase of both activators and inhibitors. The inhibitors can neutralize the activators’ increase of neighbor cells. However, because of the missing evidence about activators’ self-activation, this model is still in doubt. According to the activator–depletion model, TTG1 can move among young tissues and accumulate in cells. TTG1 binds to GL3 so that its movement will enhance the level of GL3. The cells with more GL3 and TTG1 will form trichomes [31].
Phytohormones play an important roles in the biological process of plant [32]. As has been reported, cytokinin stimulates the initiation of trichome in Arabidopsis [33]. With the external application of cytokinin, the trichome number in all rosette will significantly increase, but the length of these trichomes will be shorter. Cytokinin can also influence the endoreduplication of trichomes. The DNA content of plant, which is treated by cytokinin, is less than a plant that is grown under normal conditions [33].
microRNA (miRNA) consists of small endogenous, non-coding RNAs of only 20–22 nt in plants, animals and protozoa. Previous research has demonstrated that miRNA regulates trichome development by targeting SQUAMOSA PROMOTER BINDING PROTEIN LIKE (SPL) [34]. SPLs negatively regulate trichome initiation on stem and floral organ in Arabidopsis. SPL family has 17 members, and 10 of them are target genes of miRNA156 [35]. The expression level of miRNA156 is highest in young plants, and gradually decreases with the increase of plant age. In contrast, the expression level of SPLs is low in young plants, and increase with the increase of plant age. This expression mode is consistent with the phenotype of trichome in stem and floral organ. SPL9 decreases trichome initiation by targeting promoters of TCL1 and TRY [34]. UPL3/KAK mutation encoding HECT domain E3 ligase leads to an increase of trichome branches number and DNA content in nucleus. GL3 may be the target gene of UPL3 [36,37,38]. ETC2, an m6A reader, controls trichome branching by affecting mRNA stability in Arabidopsis [39].
C2H2 type zinc finger protein, which plays multiple functions, is one of the largest transcription factor families in Arabidopsis [40]. As shown in our previous research, GLABROUS INFLORESCENCE STEMS (GIS), GIS2, GIS3, ZINC FINGER PROTEIN 5 (ZFP5), ZFP6 and ZFP8, which all encode C2H2 zinc finger proteins, are involved in trichome initiation and root hair development, which display transcriptional hierarchy [5,41,42,43,44,45,46,47]. GIS3 integrates gibberellin (GA) and cytokinin signaling and GIS3 directly targets downstream genes, GIS and GIS2, to regulate trichome production [46]. ZFP6 also responds to gibberellin and cytokinin signaling and functions upstream of ZFP5 [45]. ZFP5 is involved in gibberellin signal transduction and acts upstream of ZFP8 [43,44]. All of them play key roles upstream of the trichome initiation complex MYB-bHLH-WD40. Hair (H), which encodes a C2H2 zinc finger protein, regulates multicellular trichome formation in tomato [48]. Recently, Han et al. found that AtZP1, which belongs to C2H2-type zinc finger protein family, negatively regulates root hair initiation and elongation. AtZP1 acts downstream of GL2 and regulates root hair initiation by targeting RHD6, RSL4 and RSL2 transcription [49]. Here, we cloned a C2H2 zinc finger protein ZFP1 (At1g80730), which is a homologous gene of ZFP5. Previous researchers have found that ZFP1 is a unique gene in the Arabidopsis genome [50] and is expressed at high levels in the shoot apex of seedling grown under a grow light [51]. It may participate in shoot development and acts downstream of photomorphogenic activation [51]. In our research, we found that ZFP1 also plays a role in controlling trichome initiation in Arabidopsis.

2. Materials and Methods

2.1. Plant Material and Growth Conditions

Ecotype Columbia (Col-0), the wild type Arabidopsis (Arabidopsis thaliana), was used as a control of experiments in this research. Plants were grown in a growth chamber with 20 °C to 22 °C, 95 to 118 μmol m−2 sec−1, 16 h light/8 h dark and 70% to 80% relative humidity. Arabidopsis seeds were sterilized with 5% (V/V) NaClO for 8 min and washed five times with sterile distilled water in laminar flow cabinet. The sterile seeds were transferred on Murashige and Skoog (MS) mediums and incubated at 4 °C for 3 days in darkness. Then, the MS mediums were placed in a growth chamber with growth conditions as described above. Trichome numbers were counted as previously described [41,42].

2.2. Isolation of zfp1 Loss-of-Function Mutant

zfp1 seeds with T-DNA insertion in the ZFP1 promoter region were ordered from Nottingham Arabidopsis Stock Centre (NASC; catalogue number N669417). Homozygous mutants were selected with kanamycin (50 mg L−1) by testing their progenies’ resistance. The presence of T-DNA insertion was confirmed by PCR with gene-specific primers (Table 1).

2.3. Construct Cloning

For overexpression and RNAi constructs, the fragments were amplified by PCR from cDNA of Col-0 using PrimeSTAR HS DNA polymerase (TaKaRa Bio Inc., Kusatsu, Japan) and inserted into a pENTR-1A vector (Invitrogen, Calsbad, CA, USA). Then, the fragments recombined into destination vectors with Gateway LR reaction (Invitrogen). The destination vectors were obtained from VIB (Flanders Interuniversity Institute for Biotechnology) as described before [43,45]. The constructs were finally transferred into Agrobacterium tumefaciens Stain GV3101 and transformed Arabidopsis by floral-dip method as described by Clough and Bent (1998) [52]. The primers used for cloning are list in Table 1.

2.4. Cytokinin Treatment and Gene Expression Analyses

For external cytokinin treatment, 6-benzylaminopurine (6BA, Sigma-Aldrich Corp., St. Louis, MO, USA) was used in this study as we described before [42,43,45]. Plants were sprayed with 100 μmol L−1 6BA and took samples after 2 h for RNA extraction when their inflorescence shoots reached 2 to 3 cm. For trichome phenotype analyses, plants were grown on soil until three to four leaves had emerged and sprayed twice a week with different concentrations of 6BA or mock solution until plants were ready for analyses as described previously [42,45].

2.5. RNA Extraction and Real-Time PCR

Total RNA was isolated with Trizol reagent (Invitrogen). Two μg of total RNA was reversely transcribed to cDNA with M-MLV transcriptase (Promega, Madison, WI, USA) as described in their instruction. The cDNA was diluted four times for real-time PCR. Real-time PCR was performed with SYBR Green PCR mix (Takara). The results of subtraction, which consisted of subtracting the Ct value of UBQ10 (At4g05320) from the Ct value of the target genes, are represented by sign ΔCt. The relative expression of the target genes was obtained by calculating 2−ΔCt as previously described [46]. The primers used in this study are listed in Table 1.

2.6. Bioinformatic Analyses

The coding sequence (CDS) and protein sequences were acquired from Tair (https://www.arabidopsis.org/). ClustalW (https://www.genome.jp/tools-bin/clustalw) was used for CDS and protein sequences alignment. Jalview software [53] was used for the alignments edit. The protein domain was predicted by SMART (https://smart.embl-heidelberg.de/).

2.7. Statistical Analyses

All data displaying in figures were analyzed by means of ANOVA. SPSS software, version 14.0 (SPSS Inc., Chicago, IL, USA), was used for the significance analysis. Student’s t-test was calculated at either 5% (*, p < 0.05) or 1% (**, p < 0.01) probability to evaluate the difference as described before [54].

3. Results

3.1. Loss of Function of ZFP1 Affects Trichome Initiation

As our previous results showed that ZFP5 plays a key role in regulating trichome initiation in Arabidopsis, we are very curious whether its homologous gene ZFP1 plays a similar role in trichome development. ZFP1, with 687 bp open reading frames encoding 228 amino acids, showed high homologous with other C2H2 zinc finger protein genes (Figure S1) [49]. We ordered zfp1 mutant (catalog No. N669417) from the Nottingham Arabidopsis Stock Centre (NASC). zfp1 carries a T-DNA insertion in the ZFP1 promoter region and was verified by genomic PCR. We also constructed an RNAi vector, which silenced the expression of ZFP1. We transferred this vector to Col-0 plants (wild type, WT) and selected ZFP1-Ri-6 line for phenotypic observation (Figure 1). To assess the function of ZFP1 in regulating trichome initiation, we counted the trichome numbers of WT, zfp1 and ZFP1-Ri-6 on their branches, cauline leaves, stem, and sepals. As shown in Figure 2A–C and Figure S2, the mutant and RNAi lines had significantly fewer trichomes than that of WT. These results indicate that ZFP1 may involve in trichome initiation in Arabidopsis.

3.2. Overexpression of ZFP1 Stimulates Trichome Initiation

To further confirm our hypothesis, we created 35S:ZFP1 transgenic lines and chose two representative overexpression lines (35S:ZFP1-6 and 35S:ZFP1-24) (Figure 1). Both lines displayed significantly higher trichomes density on second branches, second cauline leaves and stems than WT plants (Figure 2D). These results further confirm that ZFP1 positively controls trichome initiation in Arabidopsis.

3.3. ZFP1 Is Required for Cytokinin Signal to Control Trichome Initiation during Inflorescence Development

Our previous study showed that C2H2 ZINC FINGER PROTEIN could regulate trichome initiation through cytokinin signaling [42,45]. To investigate whether ZFP1 plays a similar role in regulating trichome initiation through cytokinin signaling, WT plants were sprayed with 6BA and then sampled after 2 h to test the expression level of ZFP1. As shown in Figure 3, the strong induction of a primary cytokinin response gene ARR5 after 6BA treatment indicates that the effectiveness of cytokinin application signaling. The relative expression of ZFP1 was also significantly up-regulated by 6BA treatment.
To further confirm the role of cytokinin, zfp1 mutant and wild type plants were treated with 10 μM and 100 μM 6BA. Then, we counted the trichome numbers. As shown in Figure 4, the trichome numbers increase in zfp1 plants with 6BA application, which shows a less sensitive phenotype than that of wild type plants on the second branches, second cauline leaves, and third cauline leaves. Taken together, these data indicate that ZFP1 is required for the cytokinin signal in controlling the trichome initiation in Arabidopsis.

3.4. ZFP1 Acts Upstream of Trichome Initiation Complex

Trichome initiation is mainly regulated by MYB-bHLH-WD40 complex. To verify if ZFP1 regulates trichome initiation by this pathway, we analyzed the relative expression level of GL3 and TRY. Our results showed that the expression level of GL3 in mutant and RNAi line was significantly decreased in comparison to the wild type. The expression level of negative regulator TRY was increased in mutant and RNAi line compared with wild type. In contrast, in the ZFP1 overexpression line, the expression level of GL3 was up-regulated and TRY was down-regulated compared to that of wild type (Figure 5). These results suggest indicate that ZFP1 acts upstream of the trichome initiation complex.
To further confirm this result, we over-expressed ZFP1 gene in gl3 mutant and detected if glabrous phenotype of gl3 could be restored by over-expression of ZFP1. As shown in Figure 6, 35S:ZFP1:gl3 did not rescue gl3 mutant’s phenotype. These data further support the hypothesis that ZFP1 acts upstream of trichome initiation complex.

3.5. The Expression Patterns of ZFP1

Sequence analysis displayed that ZFP1 contains a C2H2 conserved domain, which is found in all C2H2 zinc finger protein family (Figure S1). To analyze the expression pattern of ZFP1, we checked its expression level in different organs. Quantitative RT-PCR data showed that ZFP1 was mainly expressed in flower, main stem, silique and root but was expressed at lower levels in other organs (Figure 7). These results were similar with ZFP1 expression profiles extracted from Arabidopsis databases (eFP Brower, http://bar.utoronto.ca/efp_arabidopsis/cgi-bin/efpWeb.cgi) (Figure S3).

4. Discussion

Our previous results have shown that C2H2 type zinc finger protein family members, GIS, GIS2 and ZFP8, could control the trichome development in Arabidopsis, but their responses to hormonal signals are diverse [41,42,55]. GIS plays a main role in controlling the initiation on branches and GIS2 regulates the number of trichomes on flowers, while ZFP8 regulates trichomes development on cauline leaves. As shown in Figure 2, ZFP1 also plays a predominant role in branches’ trichome initiation.
Cytokinin plays a positive role in trichome initiation in Arabidopsis. The expression level of GL1, GL3, EGL3 and MYB23 is significantly induced after cytokinin application. In this study, 6BA significantly induced the expression of ZFP1 and zfp1 mutant plants were shown to be less sensitive to cytokinin than that of wild type (Figure 3 and Figure 4). At the same time, ZFP1 acts upstream of trichome initiation complex gene GL3 that is induced by cytokinin, which means ZFP1 may respond to cytokinin signaling by GL3. These results suggest that exogenous cytokinin induces the expression of ZFP1, which increases the expression of GL3 and eventually promotes trichome initiation in Arabidopsis (Figure 8). It is well-known that gibberellin participates in many growth and development processes, including seed germination, hypocotyl elongation, flowering and trichome development. GA biosynthesis mutant ga1-3 displays glabrous on leaves, and phenotypes are recovered by applying GA [56]. With the treatment of GA, the expression level of GL1, MYB23, GL3 and EGL2 is significantly increased, while the expression of TRY, ETC1 and ETC2 is significantly decreased [33]. In addition, ZFP5 responds to gibberellin and acts upstream of GIS, GIS2, ZFP8 and the trichome initiation complex [38]. Our previous study also demonstrated that ZFP6 and GIS3 could control trichome development by integrating gibberellin and cytokinin signal [45,46]. However, whether ZFP1 plays any role in control trichome development through GA signal is still unknown and needs to be further investigated.
Trichomes are specialized epidermal cells, protecting plants from abiotic and biotic stresses [43]. Our previous studies have found that ZINC FINGER PROTEIN 3 (ZFP3) participates in the responses of salt stress and osmotic stress by influencing the expression of stress-related genes and changing the proline accumulation and chlorophyll content in Arabidopsis [55]. Constitutive expression of ZFP3 enhances the tolerance of Arabidopsis to salt and osmatic stress. zfp3 loss-of-function mutants were shown to be more sensitive to salt and osmotic stress. With regards to ZFP3′s homologous gene, whether ZFP1 is also involved in abiotic stresses needs to be explored in the future.

5. Conclusions

Our results suggest that ZFP1 plays a positive role in regulating trichome initiation by acting upstream of key trichome initiation factors, GL3 and TRY, in Arabidopsis through the cytokinin pathway.

Supplementary Materials

The following are available online at https://www.mdpi.com/2077-0472/10/12/645/s1, Figure S1: Alignment of CDS sequences (A) and protein sequences (B) of ZFP1, ZFP2, ZFP3, ZFP4, ZFP5, and ZFP7 in Arabidopsis, Figure S2: Phenotype of zfp1 mutant, WT, and 35S:ZFP1. (A) Phenotype of zfp1 mutant, WT, and 35S:ZFP1 on sepal. (B) Phenotype of zfp1 mutant, WT, and 35S:ZFP1 on stem, Figure S3: Expression profiles of ZFP1 in database of public microarray data.

Author Contributions

Methodology, A.Z. and Y.G.; validation, A.Z., C.Y. and Y.L.; formal analysis, L.H. and M.W.; resources, Y.G.; data curation, A.Z. and J.W.; writing—original draft preparation, A.Z. and Y.G.; funding acquisition, Y.G. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by the National Natural Science Foundation of China (Grant number: 31570183; 31529001; 31661143004) and the National Key R & D Program of China (grand number: 2016YFD0100701).

Acknowledgments

We thank Ali Raza Khan from Zhejiang University for critical reading our manuscript and Z.Y. Qi from the Agricultural Experiment Station, Zhejiang University for his assistance during the experiment.

Conflicts of Interest

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

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Figure 1. The relative expression level of ZFP1 in zfp1, ZFP1-Ri-6 and 35S:ZFP1 lines. The values of UBQ10 were used as control to normalize the expression of target genes. Error bars indicate standard error. ** p < 0.01.
Figure 1. The relative expression level of ZFP1 in zfp1, ZFP1-Ri-6 and 35S:ZFP1 lines. The values of UBQ10 were used as control to normalize the expression of target genes. Error bars indicate standard error. ** p < 0.01.
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Figure 2. Trichome number on different organs in wild type, zfp1 mutant, ZFP1-Ri-6 and 35S:ZFP1 lines. Trichomes number on the second and third branches (A), the second and third cauline leaves (B), sepals and main stems, (C) in wild type, zfp1 mutant and ZFP1-Ri-6 line. (D) Trichomes number in wild type and 35S:ZFP1 lines. Trichomes number was the average number of counting at least 20 plants. Error bars indicate the standard error. * p < 0.05; and ** p < 0.01.
Figure 2. Trichome number on different organs in wild type, zfp1 mutant, ZFP1-Ri-6 and 35S:ZFP1 lines. Trichomes number on the second and third branches (A), the second and third cauline leaves (B), sepals and main stems, (C) in wild type, zfp1 mutant and ZFP1-Ri-6 line. (D) Trichomes number in wild type and 35S:ZFP1 lines. Trichomes number was the average number of counting at least 20 plants. Error bars indicate the standard error. * p < 0.05; and ** p < 0.01.
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Figure 3. ZFP1 expression level in wild type when treated with 100 μM 6BA. The values were normalized against the levels of UBQ10 as a control. Error bars indicate standard error. ** p < 0.01.
Figure 3. ZFP1 expression level in wild type when treated with 100 μM 6BA. The values were normalized against the levels of UBQ10 as a control. Error bars indicate standard error. ** p < 0.01.
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Figure 4. Wild type and zfp1 mutant. Response to 6BA treatment. Trichomes number on the second lateral branches (A), second (B) and third cauline leaves (C) in wild type and zfp1 mutant when treated with different concentrations of 6BA. The trichomes number was the average number of at least 20 plants. Error bars indicate the standard error.
Figure 4. Wild type and zfp1 mutant. Response to 6BA treatment. Trichomes number on the second lateral branches (A), second (B) and third cauline leaves (C) in wild type and zfp1 mutant when treated with different concentrations of 6BA. The trichomes number was the average number of at least 20 plants. Error bars indicate the standard error.
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Figure 5. Relative expression level of GL3 and TRY in wild type, zfp1 mutant and ZFP1 overexpression line. The values were normalized against the levels of UBQ10 as a control. Error bars indicate the standard error. * p < 0.05; and ** p < 0.01.
Figure 5. Relative expression level of GL3 and TRY in wild type, zfp1 mutant and ZFP1 overexpression line. The values were normalized against the levels of UBQ10 as a control. Error bars indicate the standard error. * p < 0.05; and ** p < 0.01.
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Figure 6. Genetic interaction between ZFP1 and GL3 gene. (A) Trichome initiation on the rosette leaf of 35S:ZFP1:gl3 and gl3. (B) Trichome initiation on stem of 35S:ZFP1:gl3, gl3, and wild type. Bars = 2 mm.
Figure 6. Genetic interaction between ZFP1 and GL3 gene. (A) Trichome initiation on the rosette leaf of 35S:ZFP1:gl3 and gl3. (B) Trichome initiation on stem of 35S:ZFP1:gl3, gl3, and wild type. Bars = 2 mm.
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Figure 7. Analysis of ZFP1 expression in different tissues of wild type. Ros., rosette leaf; Caul., cauline leaf; FL, flower; Mat., main stem; Sil., silique; Root, Root. The values were normalized against the levels of UBQ10 as a control.
Figure 7. Analysis of ZFP1 expression in different tissues of wild type. Ros., rosette leaf; Caul., cauline leaf; FL, flower; Mat., main stem; Sil., silique; Root, Root. The values were normalized against the levels of UBQ10 as a control.
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Figure 8. Model of the ZFP1 in regulating trichome initiation in response to cytokinin in Arabidopsis.
Figure 8. Model of the ZFP1 in regulating trichome initiation in response to cytokinin in Arabidopsis.
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Table
Table 1. Primers used for construct cloning, mutant identification and quantitative real-time PCR.
Table 1. Primers used for construct cloning, mutant identification and quantitative real-time PCR.
CodeSequenceUseReference
ZFP1-FP5′-GCCGTCGACCAATTCTTTTACTTTCATTCTCTTT-3′ZFP1 cloning
ZFP1-RP5′-AATGCGGCCGCAGTACAACTTGATCATTAGTGAAAC-3′
ZFP1-Ri-LP5′-GTCGACCCTCCTTGCCTTCTTCA-3′RNAi
ZFP1-Ri-RP5′-GCGGCCGCCTATGGTCGGTTTCTGGT-3′
zfp1-LP5′-TTTATGAGCGTTTTGGTGACC-3′mutant specific primers
zfp1-RP5′-TCAATGGTGCATATATGATTTGG-3′
LBb1.35′-ATTTTGCCGATTTCGGAAC-3′
UBQ10-RT-LP5′-GGTTCGTACCTTTGTCCAAGCA-3′qRT-PCR[40]
UBQ10-RT-RP5′-CCTTCGTTAAACCAAGCTCAGTATC-3′
ZFP1-RT-LP5′-GCGGTCACCAAAACGCTCATA-3′qRT-PCR
ZFP1-RT-RP5′-GAATCTGCTGACTGAACCGTGG-3′
GL3-RT-LP5′-GAATCTGCTGACTGAACCGTGG-3′qRT-PCR[40]
GL3-RT-RP5′-CAAGAACGTTGTCGATGTGATAATC-3′
TRY-RT-LP5′-TTGTCGGTGATAGGTGGGATTT-3′qRT-PCR[2]
TRY-RT-RP5′-ACGGTGAGGCTTGGTATGTTTG-3′
ARR5-RT-LP5′-TTGCGTCCCGAGATGTTAGAT-3′qRT-PCR[40]
ARR5-RT-RP5′-TGAGTAACCGCTCGATGAACTTC-3′
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Zhang, A.; Liu, Y.; Yu, C.; Huang, L.; Wu, M.; Wu, J.; Gan, Y. Zinc Finger Protein 1 (ZFP1) Is Involved in Trichome Initiation in Arabidopsis thaliana. Agriculture 2020, 10, 645. https://doi.org/10.3390/agriculture10120645

AMA Style

Zhang A, Liu Y, Yu C, Huang L, Wu M, Wu J, Gan Y. Zinc Finger Protein 1 (ZFP1) Is Involved in Trichome Initiation in Arabidopsis thaliana. Agriculture. 2020; 10(12):645. https://doi.org/10.3390/agriculture10120645

Chicago/Turabian Style

Zhang, Aidong, Yihua Liu, Chunyan Yu, Linli Huang, Minjie Wu, Junyu Wu, and Yinbo Gan. 2020. "Zinc Finger Protein 1 (ZFP1) Is Involved in Trichome Initiation in Arabidopsis thaliana" Agriculture 10, no. 12: 645. https://doi.org/10.3390/agriculture10120645

APA Style

Zhang, A., Liu, Y., Yu, C., Huang, L., Wu, M., Wu, J., & Gan, Y. (2020). Zinc Finger Protein 1 (ZFP1) Is Involved in Trichome Initiation in Arabidopsis thaliana. Agriculture, 10(12), 645. https://doi.org/10.3390/agriculture10120645

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